Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,513,778 G→T 11.1% T165T (ACG→ACT)  curA → curcumin/dihydrocurcumin reductase, NADPH‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,513,7780GT11.1% 976.0 / 61.5 268T165T (ACG→ACT) curAcurcumin/dihydrocurcumin reductase, NADPH‑dependent
Reads supporting (aligned to +/- strand):  ref base G (115/122);  new base T (20/11);  total (135/133)
Fisher's exact test for biased strand distribution p-value = 1.26e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 275 total aligned reads displayed.

TTGGCGATCATCCGCAAAATCCATCGTGGTCGCTGGGTGTGCTAGGGATGCCAGGCTTTACCGCTTATATGGGCCTACTGGATATCGGTCAGCCTAAAGAGGGCGAAACGTTGGTGGTAGCTGCGGCGACAGGACCAGTGGGGGCGACGGTGGGGCAAATCGGCAAACTTAAAGGTTGCAGAGTGGTGGGGGTAGCCGGTGGCGCGGAAAAATGCCGCCATGCTACCGAGGTGTTAGGCTTCGATGTTTGTCTTGATCACCACGCGGATGATTTTGCCGAACAACTGGCGAAAGCGT  >  CP009273/1513630‑1513926
                                                                                                                                                    |                                                                                                                                                    
ttGGCGATCATCCGCAAAATCCATCGTGGTCGCTGGGTGTGCTAGGGATGCCAGGCTTTACCGCTTATATGGGCCTACTGGATATCGGTCAGCCTAAAGAGGGCGAAACGTTGGTGGTAGCTGCGGCGACAGGACCAGTGGGGGCGACg                                                                                                                                                      <  1:2135977/149‑1 (MQ=255)
 tGGCGATCATCCGCAAAATCCATCGTGGTCGCTGGGTGTGCTAGGGATGCCAGGCTTTACCGCTTATATGGGCCTACTGGATATCGGTCAGCCTAAAGAGGGCGAAACGTTGGTGGTAGCTGCGGCGACAGGACCAGTGGGGGCGACgg                                                                                                                                                     >  1:2876323/1‑149 (MQ=255)
 tGGCGATCATCCGCAAAATCCATCGTGGTCGCTGGGTGTGCTAGGGATGCCAGGCTTTACCGCTTATATGGGCCTACTGGATATCGGTCAGCCTAAAGAGGGCGAAACGTTGGTGGTAGCTGCGGCGACAGGACCAGTGGGGGCGACgg                                                                                                                                                     <  1:3916315/149‑1 (MQ=255)
       tcatcCGCAAAATCCATCGTGGTCGCTGGGTGTGCTAGGGATGCCAGGCTTTACCGCTTATATGGGCCTACTGGATATCGGTCAGCCTAAAGAGGGCGAAACGTTGGTGGTAGCTGCGGCGACAGGACCAGTGGGGGCGACTGTGGGGc                                                                                                                                               >  1:26493/1‑149 (MQ=255)
        catcCGCAAAATCCATCGTGGTCGCTGGGTGTGCTAGGGATGCCAGGCTTTACCGCTTATATGGGCCTACTGGATATCGGTCAGCCTAAAGAGGGCGAAACGTTGGTGGTAGCTGCGGCGACAGGACCAGTGGGGGCGACGGTGGGGCa                                                                                                                                              <  1:3250006/149‑1 (MQ=255)
            cGCAAAATCCATCGTGGTCGCTGGGTGTGCTAGGGATGCCAGGCTTTACCGCTTATATGGGCCTACTGGATATCGGTCAGCCTAAAGAGGGCGAAACGTTGGTGGTAGCTGCGGCGACAGGACCAGTGGGGGCGACGGTGGGGCAAATc                                                                                                                                          >  2:1658784/1‑149 (MQ=255)
            cGCAAAATCCATCGTGGTCGCTGGGTGTGCTAGGGATGCCAGGCTTTACCGCTTATATGGGCCTACTGGATATCGGTCAGCCTAAAGAGGGCGAAACGTTGGTGGTAGCTGCGGCGACAGGACCAGTGGGGGCGACGGTGGGGCAAATc                                                                                                                                          <  1:4339213/149‑1 (MQ=255)
               aaaaTCCATCGTGGTCGCTGGGTGTGCTAGGGATGCCAGGCTTTACCGCTTATATGGGCCTACTGGATATCGGTCAGCCTAAAGAGGGCGAAACGTTGGTGGTAGCTGCGGCGACAGGACCAGTGGGGGCGACGGTGGGGCAAATCGGc                                                                                                                                       <  1:2250853/149‑1 (MQ=255)
               aaaaTCCATCGTGGTCGCTGGGTGTGCTAGGGATGCCAGGCTTTACCGCTTATATGGGCCTACTGGATATCGGTCAGCCTAAAGAGGGCGAAACGTTGGTGGTAGCTGCGGCGACAGGACCAGTGGGGGCGACGGTGGGGCAAATCGGc                                                                                                                                       <  2:2696323/149‑1 (MQ=255)
                 aaTCCATCGTGGTCGCTGGGTGTGCTAGGGATGCCAGGCTTTACCGCTTATATGGGCCTACTGGATATCGGTCAGCCTAAAGAGGGCGAAACGTTGGTGGTAGCTGCGGCGACAGGACCAGTGGGGGCGACGGTGGGGCAAATCGGCaa                                                                                                                                     >  1:2203611/1‑149 (MQ=255)
                  aTCCATCGTGGTCGCTGGGTGTGCTAGGGATGCCAGGCTTTACCGCTTATATGGGCCTACTGGATATCGGTCAGCCTAAAGAGGGCGAAACGTTGGTGGTAGCTGCGGCGACAGGACCAGTGGGGGCGACGGTGGGGCAAATCGGCaaa                                                                                                                                    >  2:1700475/1‑149 (MQ=255)
                   tCCATCGTGGTCGCTGGGTGTGCTAGGGATGCCAGGCTTTACCGCTTATATGGGCCTACTGGATATCGGTCAGCCTAAAGAGGGCGAAACGTTGGTGGTAGCTGCGGCGACAGGACCAGTGGGGGCGACGGTGGGGCAAATCGGCAAAc                                                                                                                                   <  2:878876/149‑1 (MQ=255)
                    ccATCGTGGTCGCTGGGTGTGCTAGGGATGCCAGGCTTTACCGCTTATATGGGCCTACTGGATATCGGTCAGCCTAAAGAGGGCGAAACGTTGGTGGTAGCTGCGGCGACAGGACCAGTGGGGGCGACGGTGGGGCAAATCGGCAAACt                                                                                                                                  <  1:994138/149‑1 (MQ=255)
                     cATCGTGGTCGCTGGGTGTGCTAGGGATGCCAGGCTTTACCGCTTATATGGGCCTACTGGATATCGGTCAGCCTAAAGAGGGCGAAACGTTGGTGGTAGCTGCGGCGACAGGACCAGTGGGGGCGACGGTGGGGCAAATCGGCAAACtt                                                                                                                                 <  1:1161646/149‑1 (MQ=255)
                       tCGTGGTCGCTGGGTGTGCTAGGGATGCCAGGCTTTACCGCTTATATGGGCCTACTGGATATCGGTCAGCCTAAAGAGGGCGAAACGTTGGTGGTAGCTGCGGCTACAGGACCAGTGGGGGCGACGGTGGGGCAAATCGGCAAACTTaa                                                                                                                               >  2:224395/1‑149 (MQ=255)
                          tGGTCGCTGGGTGTGCTAGGGATGCCAGGCTTTACCGCTTATATGGGCCTACTGGATATCGGTCAGCCTAAAGAGGGCGAAACGTTGGTGGTAGCTGCGGCGACAGGACCAGTGGGGGCGACGGTGGGGCAAATCGGCAAACTTAAAgg                                                                                                                            <  1:2783353/149‑1 (MQ=255)
                           ggTCGCTGGGTGTGCTAGGGATGCCAGGCTTTACCGCTTATATGGGCCTACTGGATATCGGTCAGCCTAAAGAGGGCGAAACGTTGGTGGTAGCTGCGGCGACAGGACCAGTGGGGGCGACGGTGGGGCAAATCGGCAAACTTAAAGGt                                                                                                                           <  1:108802/149‑1 (MQ=255)
                            gTCGCTGGGTGTGCTAGGGATGCCAGGCTTTACCGCTTAAATGGGCCTACTGGATATCGGTCAGCCTAAAGAGGGCGAAACGTTGGTGGTAGCTGCGGCGACAGGACCAGTGGGGGCGACGGTGGGGCAAATCGGCAAACTTAAAGGtt                                                                                                                          <  2:3487943/149‑1 (MQ=255)
                             tCGCTGGGTGTGCTAGGGATGCCAGGCTTTACCTCTTATATGGGCCTACTGGATATCGGTCAGCCTAAAGAGGGCGAAAAGTTGGTGGTAGCTGCGGCGACAGGACCACTGGGGGCGACGGTGGGGCAAATCGGCAAACATAAAGGTTg                                                                                                                         >  2:3255152/1‑149 (MQ=255)
                              cGCTGGGTGTGCTAGGGATGCCAGGCTTTACCGCTTATATGGGCCTACTGGATATCGGTCAGCCTAAAGAGGGCGAAACGTTGGTGGTAGCTGCGGCGACAGGACCAGTGGGGGCGACGGTGGGGCAAATCGGCAAACTTAAAGGTTGc                                                                                                                        >  1:2509986/1‑149 (MQ=255)
                              cGCTGGGTGTGCTAGGGATGCCAGGCTTTACCGCTTATATGGGCCTACTGGATATCGGTCAGCCTAAAGAGGGCGAAACGTTGGTGGTAGCTGCGGCGACAGGACCAGTGGGGGCGACGGTGGGGCAAATCGGCAAACTTAAAGGTTGc                                                                                                                        <  2:3906396/149‑1 (MQ=255)
                                 tGGGTGTGCTAGGGATGCCAGGCTTTACCGCTTATATGGGCCTACTGGATATCGGTCAGCCTAAAGAGGGCGAAACGTTGGTGGTAGCTGCGGCGACAGGACCAGTGGGGGCGACGGTGGGGCAAATCGGCAAACTTAAAGGTTGCaga                                                                                                                     <  2:2931581/149‑1 (MQ=255)
                                   ggTGTGCTAGGGATGCCAGGCTTTACCGCTTATATGGGCCTACTGGATATCGGTCAGCCTAAAGAGGGCGAAACGTTGGTGGTAGCTGCGGCGACAGGACCAGTGGGGGCGACGGTGGGGCAAATCGGCAAACTTAAAGGTTGCAGAgt                                                                                                                   <  1:304029/149‑1 (MQ=255)
                                     tgtgCTAGGGATGCCAGGCTTTACCGCTTATATGGGCCTACTGGATATCGGTCAGCCTAAAGAGGGCGAAACGTTGGTGGTAGCTGCGGCGACAGGACCAGTGGGGGCGACGGTGGGGCAAATCGGCAAACTTAAAGGTTGCAGAgtgg                                                                                                                 >  1:2111517/1‑149 (MQ=255)
                                     tgtgCTAGGGATGCCAGGCTTTACCGCTTATATGGGCCTACTGGATATCGGTCAGCCTAAAGAGGGCGAAACGTTGGTGGTAGCTGCGGCGACAGGACCAGTGGGGGCGACGGTGGGGCAAATCGGCAAACTTAAAGGTTGCAGAgtgg                                                                                                                 <  2:526626/149‑1 (MQ=255)
                                       tgCTAGGGATGCCAGGCTTTACCGCTTATATGGGCCTACTGGATATCGGTCAGCCTAAAGAGGGCGAAACGTTGGTGGTAGCTGCGGCGACAGGACCAGTGGGGGCGACGGTGGGGCAAATCGGCAAACTTAAAGGTTGCAGAgtggtg                                                                                                               <  1:4765362/149‑1 (MQ=255)
                                       tgCTAGGGATGCCAGGCTTTACCGCTTATATGGGCCTACTGGATATCGGTCAGCCTAAAGAGGGCGAAACGTTGGTGGTAGCTGCGGCGACAGGACCAGTGGGGGCGACGGTGGGGCAAATCGGCAAACTTAAAGGTTGCAGAgtggtg                                                                                                               <  1:2151603/149‑1 (MQ=255)
                                       tgCTAGGGATGCCAGGCTTTACCGCTTATATGGGCCTACTGGATATCGGTCAGCCTAAAGAGGGCGAAACGTTGGTGGTAGCTGCGGCGACAGGACCAGTGGGGGCGACGGTGGGGCAAATCGGCAAACTTAAAGGTTGCAGAgtggtg                                                                                                               <  2:2203314/149‑1 (MQ=255)
                                        gCTAGGGATGCCAGGCTTTACCGCTTATATGGGCCTACTGGATATCGGTCAGCCTAAAGAGGGCGAAACGTTGGTGGTAGCTGCGGCGACAGGACCAGTGGGGGCGACGGTGGGGCAAATCGGCAAACTTAAAGGTTGCAGAGtggtgg                                                                                                              <  1:3966425/149‑1 (MQ=255)
                                        gCTAGGGATGCCAGGCTTTACCGCTTATATGGGCCTACTGGATATCGGTCAGCCTAAAGAGGGCGAAACGTTGGTGGTAGCTGCGGCGACAGGACCAGTGGGGGCGACGGTGGGGCAAATCGGCAAACTTAAAGGTTGCAGAGtggtgg                                                                                                              <  2:330644/149‑1 (MQ=255)
                                        gCTAGGGATGCCAGGCTTTACCGCTTATATGGGCCTACTGGATATCGGTCAGCCTAAAGAGGGCGAAACGTTGGTGGTAGCTGCGGCGACAGGACCAGTGGGGGCGACGGTGGGGCAAATCGGCAAACTTAAAGGTTGCAGAGtggtgg                                                                                                              <  2:4825656/149‑1 (MQ=255)
                                         cTAGGGATGCCAGGCTTTACCGCTTATATGGGCCTACTGGATATCGGTCAGCCTAAAGAGGGCGAAACGTTGGTGGTAGCTGCGGCGACAGGACCAGTGGGGGCGACGGTGGGGCAAATCGGCAAACTTAAAGGTTGCAGAGTGGTggg                                                                                                             >  2:4256737/1‑149 (MQ=255)
                                           aGGGATGCCAGGCTTTACCGCTTATATGGGCCTACTGGATATCGGTCAGCCTAAAGAGGGCGAAACGTTGGTGGTAGCTGCGGCGACAGGACCAGTGGGGGCGACTGTGGGGCAAATCGGCAAACTTAAAGGTTGCAGAGTGGTggggg                                                                                                           >  2:1307099/1‑149 (MQ=255)
                                            gggATGCCAGGCTTTACCGCTTATATGGGCCTACTGGATATCGGTCAGCCTACAGAGGGCGACACGTTGGTGGTAGCTGCGGCGACAGGACCAGTGGGGGCGACGGTGGGGCAAATCGGCAAACTTAAAGGTTGCAGAGTGGTGGGGGt                                                                                                          <  1:3099976/149‑1 (MQ=255)
                                            gggATGCCAGGCTTTACCGCTTATATGGGCCTACTGGATATCGGTCAGCCTAAAGAGGGCGAAACGTTGGTGGTAGCTGCGGCGACAGGACCAGTGGGGGCGACGGTGGGGCAAATCGGCAAACTTAAAGGTTGCAGAGTGGTGGGGGt                                                                                                          >  1:2477292/1‑149 (MQ=255)
                                             ggttGCCAGGCTTTACCGCTTATATGGGCCTACTGGATATCGGTCAGCCTAATGAGGGCGAAACGTTGGTGGTAGCTGCGGCGACAGGACCAGTGGGGGCGACGGTGGGGCAAATCGGCAAACTTAAAGGTTGCAGAGTGGTGGGGGTa                                                                                                         >  1:2014458/4‑149 (MQ=255)
                                               aTGCCAGGCTTTACCGCTTATATGGGCCTACTGGATATCGGTCAGCCTAAAGAGGGCGAAACGTTGGTGGTAGCTGCGGCGACTGGAACTGTGTGGGCGACTGTTGGGCAAATCGGCAAACTTATAGGTTGCAGTGTGGTGGGGGTAGc                                                                                                       <  2:113691/149‑1 (MQ=255)
                                               aTGCCAGGCTTTACCGCTTATATGGGCCTACTGGATATCGGTCAGCCTAAAGAGGGCGAAACGTTGGTGGTAGCTGCGGCGACAGGACCAGTGGGGGCGACGGTGGGGCAAATCGGCAAACTTAAAGGTTGCAGAGTGGTGGGGGTAGc                                                                                                       <  1:4713385/149‑1 (MQ=255)
                                                tGCCAGGCTTTACCGCTTATATGGGCCTACTGGATATCGGTCAGCCTAAAGAGGGCGAAACGTTGGTGGTAGCTGCGGCGACAGGACCAGTGGGGGCGACGGTGGGGCAAATCGGCAAACTTAAAGGTTGCAGAGTGGTGGGGGTAGcc                                                                                                      <  1:561032/149‑1 (MQ=255)
                                                    aGGCTTTACCGCTTATATGGGCCTACTGGATATCGGTCAGCCTAAAGAGGGCGAAACGTTGGTGGTAGCTGCGGCGACAGGACCAGTGGGGGCGACGGTGGGGCAAATCGGCAAACTTAAAGGTTGCAGAGTGGTGGGGGTAGCCGGTg                                                                                                  >  2:456876/1‑149 (MQ=255)
                                                     ggCTTTACCGCTTATATGGGCCTACTGGATATCGGTCAGCCTAAAGAGGGCGAAACGTTGGTGGTAGCTGCGGCGACAGGACCAGTGGGGGCGACGGTGGGGCAAATCGGCAAACTTAAAGGTTGCAGAGTGGTGGGGGTAGCCGGTgg                                                                                                 >  2:4736114/1‑149 (MQ=255)
                                                      gCTTTACCGCTTATATGGGCCTACTGGATATCGGTCAGCCTAAAGAGGGCGAAACGTTGGTGGTAGCTGCGGCGACAGGACCAGTGGGGGCGACGGTGGGGCAAATCGGCAAACTTAAAGGTTGCAGAGTGGTGGGGGTAGCCGGTGgc                                                                                                >  2:26231/1‑149 (MQ=255)
                                                        tttACCGCTTATATGGGCCTACTGGATATCGGTCAGCCTAAAGAGGGCGAAACGTTGGTGGTAGCTGCGGCGACAGGACCAGTGGGGGCGACGGTGGGGCAAATCGGCAAACTTAAAGGTTGCAGAGTGGTGGGGGTAGCCGGTGgcgc                                                                                              >  1:1389322/1‑149 (MQ=255)
                                                          tACCGCTTATATGGGCCTACTGGATATCGGTCAGCCTAAAGAGGGCGAAACGTTGGTGGTAGCTGCGGCGACAGGACCAGTGGGGGCGACGGTGGGGCAAATCGGCAAACTTAAAGGTTGCAGAGTGGTGGGGGTAGCCGGTGGCGCgg                                                                                            <  1:730346/149‑1 (MQ=255)
                                                            ccGCTTATATGGGCCTACTGGATATCGGTCAGCCTAAAGAGGGCGAAACGTTGGTGGTAGCTGCGGCGACAGGACCAGTGGGGGCGACGGTGGGGCAAATCGGCAAACTTAAAGGTTGCAGAGTGGTGGGGGTAGCCGGTGGCGCGGaa                                                                                          >  2:3920014/1‑149 (MQ=255)
                                                             cGCTTATATGGGCCTACTGGATATCGGTCAGCCTAAAGAGGGCGAAACGTTGGTGGTAGCTGCGGCGACAGGACCAGTGGGGGCGACGGTGGGGCAAATCGGCAAACTTAAAGGTTGCAGAGTGGTGGGGGTAGCCGGTGGCGCGGaaa                                                                                         >  2:3194140/1‑149 (MQ=255)
                                                               cTTATATGGGCCTACTGGATATCGGTCAGCCTAAAGAGGGCGAAACGTTGGTGGTAGCTGCGGCGACAGGACCAGTGGGGGCGACGGTGGGGCAAATCGGCAAACTTAAAGGTTGCAGAGTGGTGGGGGTAGCCGGTGGCGCGGaaaaa                                                                                       >  2:4041084/1‑149 (MQ=255)
                                                               cTTATATGGGCCTACTGGATATCGGTCAGCCTAAAGAGGGCGAAACGTTGGTGGTAGCTGCGGCGACAGGACCAGTGGGGGCGACGGTGGGGCAAATCGGCAAACTTAAAGGTTGCAGAGTGGTGGGGGTAGCCGGTGGCGCGGaaaaa                                                                                       >  1:3585377/1‑149 (MQ=255)
                                                                   tatGGGCCTACTGGATATCGGTCAGCCTAAAGAGGGCGAAACGTTGGTGGTAGCTGCGGCGACAGGACCAGTGGGGGCGACGGTGGGGCAAATCGGCAAACTTAAAGGTTGCAGAGTGGTGGGGGTAGCCGGTGGCGCGGAAAAATgcc                                                                                   >  1:796414/1‑149 (MQ=255)
                                                                   tatGGGCCTACTGGATATCGGTCAGCCTAAAGAGGGCGAAACGTTGGTGGTAGCTGCGGCGACAGGACCAGTGGGGGCGACGGTGGGGCAAATCGGCAAACTTAAAGGTTGCAGAGTGGTGGGGGTAGCCGGTGGCGCGGAAAAATgcc                                                                                   >  2:5224604/1‑149 (MQ=255)
                                                                     tGGGCCTACTGGATATCGGTCAGCCTAAAGAGGGCGAAACGTTGGTGGTAGCTGCGGCGACAGGACCAGTGGGGGCGACGGTGGGGCAAATCGGCAAACTTAAAGGTTGCAGAGTGGTGGGGGTAGCCGGTGGCGCGGAAAAATgccgc                                                                                 <  1:5120797/149‑1 (MQ=255)
                                                                        gCCTACTGGATATCGGTCAGCCTAAAGAGGGCGAAACGTTGGTGGTAGCTGCGGCGACAGGACCAGTGGGGGCGACGGTGGGGCAAATCGGCAAACTTAAAGGTTGCAGAGTGGTGGGGGTAGCCGGTGGCGCGGAAAAATGCCGCCAt                                                                              <  2:4744375/149‑1 (MQ=255)
                                                                        gCCTACTGGATATCGGTCAGCCTAAAGAGGGCGAAACGTTGGTGGTAGCTGCGGCGACAGGACCAGTGGGGGCGACGGTGGGGCAAATCGGCAAACTTAAAGGTTGCAGAGTGGTGGGGGTAGCCGGTGGCGCGGAAAAATGCCGCCAt                                                                              <  2:2569652/149‑1 (MQ=255)
                                                                          cTACTGGATATCGGTCAGCCTAAAGAGGGCGAAACGTTGGTGGTAGCTGCGGCGACAGGACCAGTGGGGGCGACGGTGGGGCAAATCGGCAAACTTAAAGGTTGCAGAGTGGTGGGGGTAGCCGGTGGCGCGGAAAAATGCCGCCATGc                                                                            <  1:1717797/149‑1 (MQ=255)
                                                                          cTACTGGATATCGGTCAGCCTAAAGAGGGCGAAACGTTGGTGGTAGCTGCGGCGACAGGACCAGTGGGGGCGACGGTGGGGCAAATCGGCAAACTTAAAGGTTGCAGAGTGGTGGGGGTAGCCGGTGGCGCGGAAAAATGCCGCCATGc                                                                            >  1:3382971/1‑149 (MQ=255)
                                                                             cTGTATATCGGTCAGCCTAAAGAGGGCGAAACGTTGGTGGTAGCTGCGGCGACAGGACCAGTGGGGGCGACGGTGGGGCAAATCGGCAAACTTAAAGGTTGCAGAGTGGTGGGGGTAGCCGGTGGCGCGGAAAAATGCCGCCATGCTAc                                                                         <  1:3300229/149‑1 (MQ=255)
                                                                             cTGGATATCGGTCAGCCTAAAGAGGGCGAAACGTTGGTGGTAGCTGCGGCGACAGGACCAGTGGGGGCGACTGTGGGGCAAATCGGCAAACTTAAAGGTTGCAGAGTGGTGGGGGTAGCCGGTGGCGCGGAAAAATGCCGCCATGCTAc                                                                         >  1:4041263/1‑149 (MQ=255)
                                                                             cTGGATATCGGTCAGCCTAAAGAGGGCGAAACGTTGGTGGTAGCTGCGGCGACAGGACCAGTGGGGGCGACGGTGGGGCAAATCGGCAAACTTAAAGGTTGCAGAGTGGTGGGGGTAGCCGGTGGCGCGGAAAAATGCCGCCATGCTAc                                                                         <  1:1899952/149‑1 (MQ=255)
                                                                             cTGGATATCGGTCAGCCTAAAGAGGGCGAAACGTTGGTGGTAGCTGCGGCGACAGGACCAGTGGGGGCGACGGTGGGGCAAATCGGCAAACTTAAAGGTTGCAGAGTGGTGGGGGTAGCCGGTGGCGCGGAAAAATGCCGCCATGCTAc                                                                         <  1:1896309/149‑1 (MQ=255)
                                                                                gATATCGGTCAGCCTAAAGAGGGCGAAACGTTGGTGGTAGCTGCGGCGACAGGACCAGTGGGGGCGACGGTGGGGCAAATCGGCAAACTTAAAGGTTGCAGAGTGGTGGGGGTAGCCGGTGGCGCGGAAAAATGCCGCCATGCTACCGa                                                                      <  2:1957956/149‑1 (MQ=255)
                                                                                gATATCGGTCAGCCTAAAGAGGGCGAAACGTTGGTGGTAGCTGCGGCGACAGGACCAGTGGGGGCGACGGTGGGGCAAATCGGCAAACTTAAAGGTTGCAGAGTGGTGGGGGTAGCCGGTGGCGCGGAAAAATGCCGCCATGCTACCGa                                                                      <  2:619201/149‑1 (MQ=255)
                                                                                 atatCGGTCAGCCTAAAGAGGGCGAAACGTTGGTGGTAGCTGCGGCGACAGGACCAGTGGGGGCGACGGTGGGGCAAATCGGCAAACTTAAAGGTTGCAGAGTGGTGGGGGTAGCCGGTGGCGCGGAAAAATGCCGCCATGCTACCGAg                                                                     >  2:379097/1‑149 (MQ=255)
                                                                                   atCGGTCAGCCTAAAGAGGGCGAAACGTTGGTGGTAGCTGCGGCGACAGGACCAGTGGGGGCGACGGTGGGGCAAATCGGCAAACTTAAAGGTTGCAGAGTGGTGGGGGTAGCCGGTGGCGCGGAAAAATGCCGCCATGCTACCGAGgt                                                                   >  1:1594497/1‑149 (MQ=255)
                                                                                   atCGGTCAGCCTAAAGAGGGCGAAACGTTGGTGGTAGCTGCGGCGACAGGACCAGTGGGGGCGACGGTGGGGCAAATCGGCAAACTTAAAGGTTGCAGAGTGGTGGGGGTAGCCGGTGGCGCGGAAAAATGCCGCCATGCTACCGAGgt                                                                   >  2:4411601/1‑149 (MQ=255)
                                                                                    tCGGTCAGCCTAAAGAGGGTGAAACGTTGGTGGTAGCTGCGGCGACAGGACCAGTGGGGGCGACGGTGGGGCAAATCGGCAAACTTAAAGGTTGCAGAGTGGTGGGGGTAGCCGGTGGCGCGGAAAAATGCCGCCATGCTACCGAGgtg                                                                  >  2:5141598/1‑149 (MQ=255)
                                                                                     cGGTCAGCCTAAAGAGGGCGAAACGTTGGTGGTAGCTGCGGCGACAGGACCAGTGGGGGCGACTGTGGGGCAAATCGGCAAACTTAAAGGTTGCAGAGTGGTGGGGGTAGCCGGTGGCGCGGAAAAATGCCGCCATGCTACCGAGgtgt                                                                 <  1:1257624/149‑1 (MQ=255)
                                                                                     cGGTCAGCCTAAAGAGGGCGAAACGTTGGTGGTAGCTGCGGCGACAGGACCAGTGGGGGCGACGGTGGGGCAAATCGGCAAACTTAAAGGTTGCAGAGTGGTGGGGGTAGCCGGTGGCGCGGAAAAATGCCGCCATGCTACCGAGgtgt                                                                 >  1:1503237/1‑149 (MQ=255)
                                                                                      ggTCAGCCTAAAGAGGGCGAAACGTTGGTGGTAGCTGCGGCGACAGGACCAGTGGGGGCGACGGTGGGGCAAATCGGCAAACTTAAAGGTTGCAGAGTGGTGGGGGTAGCCGGTGGCGCGGAAAAATGCCGCCATGCTACCGAGGTGtt                                                                <  1:4060315/149‑1 (MQ=255)
                                                                                       gTCAGCCTAAAGAGGGCGAAACGTTGGTGGTAGCTGCGGCGACAGGACCAGTGGGGGCGACGGTGGGGCAAATCGGCAAACTTAAAGGTTGCAGAGTGGTGGGGGTAGCCGGTGGCGCGGAAAAATGCCGCCATGCTACCGAGGTGTTa                                                               <  2:2604890/149‑1 (MQ=255)
                                                                                       gTCAGCCTAAAGAGGGCGAAACGTTGGTGGTAGCTGCGGCGACAGGACCAGTGGGGGCGACGGTGGGGCAAATCGGCAAACTTAAAGGTTGCAGAGTGGTGGGGGTAGCCGGTGGCGCGGAAAAATGCCGCCATGCTACCGAGGTGTTa                                                               <  2:2016466/149‑1 (MQ=255)
                                                                                       gTCAGCCTAAAGAGGGCGAAACGTTGGTGGTAGCTGCGGCGACAGGACCAGTGGGGGCGACGGTGGGGCAAATCGGCAAACTTAAAGGTTGCAGAGTGGTGGGGGTAGCCGGTGGCGCGGAAAAATGCCGCCATGCTACCGAGGTGTTa                                                               >  2:1873384/1‑149 (MQ=255)
                                                                                        tCAGCCTAAAGAGGGCGAAACGTTGGTGGTAGCTGCGGCGACAGGACCAGTGGGGGCGACGGTGGGGCAAATCGGCAAACTTAAAGGTTGCAGAGTGGTGGGGGTAGCCGGTGGCGCGGAAAAATGCCGCCATGCTACCGAGGTGTTAg                                                              <  2:1888183/149‑1 (MQ=255)
                                                                                          aGCCTAAAGAGGGCGAAACGTTGGTGGTAGCTGCGGCGACAGGACCAGTGGGGGCGACGGTGGGGCAAATCGGCAAACTTAAAGGTTGCAGAGTGGTGGGGGTAGCCGGTGGCGCGGAAAAATGCCGCCATGCTACCGAGGTGTTAGGc                                                            <  1:3478898/149‑1 (MQ=255)
                                                                                           gCCTAAAGAGGGCGAAACGTTGGTGGTAGCTGCGGCGACAGGACCAGTGGGGGCGACGGTGGGGCAAATCGGCAAACTTAAAGGTTGCAGAGTGGTGGGGGTAGCCGGTGGCGCGGAAAAATGCCGCCATGCTACCGAGGTGTTAGGCt                                                           >  1:1029864/1‑149 (MQ=255)
                                                                                            ccTAAAGAGGGCGAAACGTTGGTGGTAGCTGCGGCGACAGGACCAGTGGGGGCGACGGTGGGGCAAATCGGCAAACTTAAAGGTTGCAGAGTGGTGGGGGTAGCCGGTGGCGCGGAAAAATGCCGCCATGCTACCGAGGTGTTAGGCtt                                                          <  1:236821/149‑1 (MQ=255)
                                                                                              tAAAGAGGGCGAAACGTTGGTGGTAGCTGCGGCGACAGGACCAGTGGGGGCGACGGTGGGGCAAATCGGCAAACTTAAAGGTTGCAGAGTGGTGGGGGTAGCCGGTGGCGCGGAAAAATGCCGCCATGCTACCGAGGTGTTAGGCTTCg                                                        >  1:3727652/1‑149 (MQ=255)
                                                                                                aaGAGGGCGAAACGTTGGTGGTAGCTGCGGCGACAGGACCAGTGGGGGCGACGGTGGGGCAAATCGGCAAACTTAAAGGTTGCAGAGTGGTGGGGGTAGCCGGTGGCGCGGAAAAATGCCGCCATGCTACCGAGGTGTTAGGCTTCGAt                                                      <  1:4454212/149‑1 (MQ=255)
                                                                                                aaGAGGGCGAAACGTTGGTGGTAGCTGCGGCGACAGGACCAGTGGGGGCGACGGTGGGGCAAATCGGCAAACTTAAAGGTTGCAGAGTGGTGGGGGTAGCCGGTGGCGCGGAAAAATGCCGCCATGCTACCGAGGTGTTAGGCTTCGAt                                                      <  1:4513787/149‑1 (MQ=255)
                                                                                                aaGAGGGCGAAACGTTGGTGGTAGCTGCGGCGACAGGACCAGTGGGGGCGACGGTGGGGCAAATCGGCAAACTTAAAGGTTGCAGAGTGGTGGGGGTAGCCGGTGGCGCGGAAAAATGCCGCCATGCTACCGAGGTGTTAGGCTTCGAt                                                      >  2:24751/1‑149 (MQ=255)
                                                                                                 agagGGCGAAACGTTGGTGGTAGCTGCGGCGACAGGACCAGTGGGGGCGACTGTGGGGCAAATCGGCAAACTTAAAGGTTGCAGAGTGGTGGGGGTAGCCGGTGGCGCGGAAAAATGCCGCCATGCTACCGAGGTGTTAGGCTTCGATg                                                     >  1:496142/1‑149 (MQ=255)
                                                                                                  gagGGCGAAACGTTGGTGGTAGCTGCGGCGACAGGACCAGTGGGGGCGACGGTGGGGCAAATCGGCAAACTTAAAGGTTGCAGAGTGGTGGGGGTAGCCGGTGGCGCGGAAAAATGCCGCCATGCTACCGAGGTGTTAGGCTTCGATGt                                                    <  1:930210/149‑1 (MQ=255)
                                                                                                          aaCGTTGGTGGTAGCTGCGGCGACAGGACCAGTGGGGGCGACGGTGGGGCAAATCGGCAAACTTAAAGGTTGCAGAGTGGTGGGGGTAGCCGGTGGCGCGGAAAAATGCCGCCATGCTACCGAGGTGTTAGGCTTCGATGTTTGTCTTg                                            >  2:3947817/1‑149 (MQ=255)
                                                                                                            cGTTGGTGGTAGCTGCGGCGACAGGACCAGTGGGGGCGACGGTGGGGCAAATCGGCAAACTTAAAGGTTGCAGAGTGGTGGGGGTAGCCGGTGGCGCGGAAAAATGCCGCCATGCTACCGAGGTGTTAGGCTTCGATGTTTGTCTTGAt                                          <  2:1928678/149‑1 (MQ=255)
                                                                                                             gTTGGTGGTAGCTGCGGCTACAGGACCAGTGGGGGCGACGGTGGGGCAAATCGGCAAACTTAAAGGTTGCAGAGTGGTGGGGGTAGCCGGTGGCGCGGAAAAATGCCGCCATGCTACCGAGGTGTTAGGCTTCGATGTTTGTCTTGATc                                         <  1:2739252/149‑1 (MQ=255)
                                                                                                             gTTGGTGGTAGCTGCGGCGACAGGACCAGTGGGGGCGACGGTGGGGCAAATCGGCAAACTTAAAGGTTGCAGAGTGGTGGGGGTAGCCGGTGGCGCGGAAAAATGCCGCCATGCTACCGAGGTGTTAGGCTTCGATGTTTGTCTTGATc                                         >  2:2294402/1‑149 (MQ=255)
                                                                                                                 gtggtAGCTGCGGCGACAGGACCAGTGGGGGCGACTGTGGGGCAAATCGGCAAACTTAAAGGTTGCAGAGTGGTGGGGGTAGCCGGTGGCGCGGAAAAATGCCGCCATGCTACCGAGGTGTTAGGCTTCGATGTTTGTCTTGATcacca                                     <  1:5249386/149‑1 (MQ=255)
                                                                                                                  tggtAGCTGCGGCGACAGGACCAGTGGGGGCGACGGTGGGGCAAATCGGCAAACTTAAAGGTTGCAGAGTGGTGGGGGTAGCCGGTGGCGCGGAAAAATACCGCCATGCTACCGAGGTGTTAGGCTTCGATGTTTGTCTTGATcaccac                                    >  1:2068652/1‑149 (MQ=255)
                                                                                                                   ggtAGCTGCGGCGACAGGACCAGTGGGGGCGACGGTGGGGCAAATCGGCAAACTTAAAGGTTGCAGAGTGGTGGGGGTAGCCGGTGGCGCGGAAAAATGCCGCCATGCTACCGAGGTGTTAGGCTTCGATGTTTGTCTTGATCACCAcg                                   >  2:3601270/1‑149 (MQ=255)
                                                                                                                     tAGCTGCGGCGACAGGACCAGTGGGGGCGACGGTGGGGCAAATCGGCAAACTTAAAGGTTGCAGAGTGGTGGGGGTAGCCGGTGGCGCGGAAAAATGCCGCCATGCTACCGAGGTGTTAGGCTTCGATGTTTGTCTTGATCACCAcgcg                                 <  2:4919241/149‑1 (MQ=255)
                                                                                                                         tGCGGCGACAGGACCAGTGGGGGCGACGGTGGGGCAAATCGGCAAACTTAAAGGTTGCAGAGTGGTGGGGGTAGCCGGTGGCGCGGAAAAATGCCGCCATGCTACCGAGGTGTTAGGCTTCGATGTTTGTCTTGATCACCACGCGgatg                             <  2:4418941/149‑1 (MQ=255)
                                                                                                                           cggcgACAGGACCAGTGGGGGCGACGGTGGGGCAAATCGGCAAACTTAAAGGTTGCAGAGTGGTGGGGGTAGCCGGTGGCGCGGAAAAATGCCGCCATGCTACCGAGGTGTTAGGCTTCGATGTTTGTCTTGATCACCACGCGgatgat                           <  1:3699588/149‑1 (MQ=255)
                                                                                                                             gctaCAGGACCAGTGGGGGCGACGGTGGGGCAAATCGGCAAACTTAAAGGTTGCAGAGTGGTGGGGGTAGCCGGTGGCGCGGAAAAATGCCGCCATGCTACCGAGGTGTTAGGCTTCGATGTTTGTCTTGATCACCACGCGGATGAttt                         <  2:3029540/146‑1 (MQ=255)
                                                                                                                              cgACAGGACCAGTGGGGGCGACGGTGGGGCAAATCGGCAAACTTAAAGGTTGCAGAGTGGTGGGGGTAGCCGGTGGCGCGGAAAAATGCCGCCATGCTACCGAGGTGTTAGGCTTCGATGTTTGTCTTGATCACCACGCGGATGAtttt                        <  2:2735212/149‑1 (MQ=255)
                                                                                                                              cgACAGGACCAGTGGGGGCGACGGTGGGGCAAATCGGCAAACTTAAAGGTTGCAGAGTGGTGGGGGTAGCCGGTGGCGCGGAAAAATGCCGCCATGCTACCGAGGTGTTAGGCTTCGATGTTTGTCTTGATCACCACGCGGATGAtttt                        <  2:2735024/149‑1 (MQ=255)
                                                                                                                                aCAGGACCAGTGGGGGCGACTGTGGGGCAAATCGGCAAACTTAAAGGTTGCAGAGTGGTGGGGGTAGCCGGTGGCGCGGAAAAATGCCGCCATGCTACCGAGGTGTTAGGCTTCGATGTTTGTCTTGATCACCACGCGGATGATTTTGc                      >  1:3838663/1‑149 (MQ=255)
                                                                                                                                  aGGACCAGTGGGGGCGACGGTGGGGCAAATCGGCAAACTTAAAGGTTGCAGAGTGGTGGGGGTAGCCGGTGGCGCGGAAAAATGCCGCCATGCTACCGAGGTGTTAGGCTTCGATGTTTGTCTTGATCACCACGCGGATGATTTTGCCg                    <  2:1588160/149‑1 (MQ=255)
                                                                                                                                   ggACCAGTGGGGGCGACTGTGGGGCAAATCGGCAAACTTAAAGGTTGCAGAGTGGTGGGGGTAGCCGGTGGCGCGGAAAAATGCCGCCATGCTACCGAGGTGTTAGGCTTCGATGTTTGTCTTGATCACCACGCGGATGATTTTGCCGa                   >  2:1616624/1‑149 (MQ=255)
                                                                                                                                                  aCGGTGGGGCAAATCGGCAAACTTAAAGGTTGCAGAGTGGTGGGGGTAGCCGGTGGCGCGGAAAAATGCCGCCATGCTACCGAGGTGTTAGGCTTCGATGTTTGTCTTGATCACCACGCGGATGATTTTGCCGAACAACTGGCGAAAGc    <  2:2848252/149‑1 (MQ=255)
                                                                                                                                                   cGGTGGGGCAAATCGGCAAACTTAAAGGTTGCAGAGTGGTGGGGGTAGCCGGTGGCGCGGAAAAATGCCGCCATGCTACCGAGGTGTTAGGCTTCGATGTTTGTCTTGATCACCACGCGGATGATTTTGCCGAACAACTGGCGAAAGCg   <  1:419935/149‑1 (MQ=255)
                                                                                                                                                    ggTGGGGCAAATCGGCAAACTTAAAGGTTGCAGAGTGGTGGGGGTAGCCGGTGGCGCGGAAAAATGCCGCCATGCTACCGAGGTGTTAGGCTTCGATGTTTGTCTTGATCACCACGCGGATGATTTTGCCGAACAACTGGCGAAAGCGt  <  2:1182991/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
TTGGCGATCATCCGCAAAATCCATCGTGGTCGCTGGGTGTGCTAGGGATGCCAGGCTTTACCGCTTATATGGGCCTACTGGATATCGGTCAGCCTAAAGAGGGCGAAACGTTGGTGGTAGCTGCGGCGACAGGACCAGTGGGGGCGACGGTGGGGCAAATCGGCAAACTTAAAGGTTGCAGAGTGGTGGGGGTAGCCGGTGGCGCGGAAAAATGCCGCCATGCTACCGAGGTGTTAGGCTTCGATGTTTGTCTTGATCACCACGCGGATGATTTTGCCGAACAACTGGCGAAAGCGT  >  CP009273/1513630‑1513926

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑