BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	1,579,818	A→T	5.7%	L309* (TTG→TAG)	ydeP ←	putative oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	1,579,818	0	A	T	5.7%	1240.5 / 48.2	342	L309* (TTG→TAG)	ydeP	putative oxidoreductase
Reads supporting (aligned to +/- strand): ref base A (180/142); new base T (12/8); total (192/150)
Fisher's exact test for biased strand distribution p-value = 8.19e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 350 total aligned reads displayed.

GGCAGCGGCATATGCGTCAGCCAGTTCGGCGATTTGTGTCTGACTTAGTCCAGAAATACGTTCGATATCTTTCCACTCGGAATTGAGAACGTCACGGCGTAGCTCGTCAAAGCCGACGGTATGCGTTTGAATAAACTCGTCATCAAGCAATGAAGGCCGACCTGCGGCGCTTGCAGCATCATCGCGCTCAATTAACAGGCGCATCATCCCCTTGAGCAACGCCATATCGCCACCAATGCGCACGTTATAGTAGGCACTGGCCAACTGAGTCTCAGAGTTCGTCAGCATTTCAAACGG  >  CP009273/1579670‑1579966

ggCAGCGGCATATGCGTCAGCCAGTTCGGCGATTTGTGTCTGACTTAGTCCAGAAATACGTTCGATATCTTTCCACTCGGAATTGAGAACGTCACGGCGTAGCTCGTCAAAGCCGACGGTATGCGTTTGAATAAACTCGTCATCAAGCa                                                                                                                                                      >  1:1896796/1‑149 (MQ=255)

    gCGGCATATGCGTCAGCCAGTTCGGCGATTTGTGTCTGACTTAGTCCAGAAATACGTTCGATATCTTTCCACTCGGAATTGAGAACGTCACGGCGTAGCTCGTCAAAGCCGACGGTATGCGTTTGAATAAACTCGTCATCAAGCAATGa                                                                                                                                                  >  2:491131/1‑149 (MQ=255)

       gCATATGCGTCAGCCAGTTCGGCGATTTGTGTCTGACTTAGTCCAGAAATACGTTCGATATCTTTCCACTCGGAATTGAGAACGTCACGGCGTAGCTCGTCAAAGCCGACGGTATGCGTTTGAATAAACTCGTCATCAAGCAATGAAgg                                                                                                                                               <  2:456119/149‑1 (MQ=255)

        cATATGCGTCAGCCAGTTCGGCGATTTGTGTCTGACTTAGTCCAGAAATACGTTCGATATCTTTCCACTCGGAATTGAGAACGTCACGGCGTAGCTCGTCAAAGCCGACGGTATGCGTTTGAATAAACTCGTCATCAAGCAATGAAGGc                                                                                                                                              >  1:125391/1‑149 (MQ=255)

         atatGCGTCAGCCAGTTCGGCGATTTGTGTCTGACTTAGTCCAGAAATACGTTCGATATCTTTCCACTCGGAATTGAGAACGTCACGGCGTAGCTCGTCAAAGCCGACGGTATGCGTTTGAATAAACTCGTCATCAAGCAATGAAGGcc                                                                                                                                             >  2:2889551/1‑149 (MQ=255)

         atatGCGTCAGCCAGTTCGGCGATTTGTGTCTGACTTAGTCCAGAAATACGTTCGATATCTTTCCACTCGGAATTGAGAACGTCACGGCGTAGCTCGTCAAAGCCGACGGTATGCGGTTGAATAAACTCGTCATCAAGCAATGAAGGcc                                                                                                                                             >  2:3737240/1‑149 (MQ=255)

          tatGCGTCAGCCAGTTCGGCGATTTGTGTCTGACTTAGTCCAGAAATACGTTCGATATCTTTCCACTCGGAATTGAGAACGTCACGGCGTAGCTCGTCAAAGCCGACGGTATGCGTTTGAATAAACTCGTCATCAAGCAATGAAGGcag                                                                                                                                            >  1:1426744/1‑147 (MQ=255)

           atGCGTCAGCCAGTTCGGCGATTTGTGTCTGACTTAGTCCAGAAATACGTTCGATATCTTTCCACTCGGAATTGAGAACGTCACGGCGTAGCTCGTCAAAGCCGACGGTATGCGTTTGAATAAACTCGTCATCAAGCAATGAAGGCCGa                                                                                                                                           <  1:2347317/149‑1 (MQ=255)

            tGCGTCAGCCAGTTCGGCGATTTGTGTCTGACTTAGTCCAGAAATACGTTCGATATCTTTCCACTCGGAATTGAGAACGTCACGGCGTAGCTCGTCAAAGCCGACGGTATGCGTTTGAATAAACTCGTCATCAAGCAATGAAGGCCGAc                                                                                                                                          >  1:1565854/1‑149 (MQ=255)

                tCAGCCAGTTCGGCGATTTGTGTCTGACTTAGTCCAGAAATACGTTCGATATCTTTCCACTCGGAATTGAGAACGTCACGGCGTAGCTCGTCAAAGCCGACGGTATGCGTTTGAATAAACTCGTCATCAAGCAATGAAGGCCGACCTgc                                                                                                                                      <  1:1799063/149‑1 (MQ=255)

                 cAGCCAGTTCGGCGATTTGTGTCTGACTTAGTCCAGAAATACGTTCGATATCTTTCCACTCGGAATTGAGAACGTCACGGCGTAGCTCGTCAAAGCCGACGGTATGCGTTTGAATAAACTCGTCATCAAGCAATGAAGGCCGACCTgcg                                                                                                                                     >  2:679868/1‑149 (MQ=255)

                   gCCAGTTCGGCGTTTTTTGTCTGACTTAGTCCAGAAATACGTTCGATATCTTTCCACTCTGAATTGAGAACGTCACGGCGTAGCTAGTCAAAGCCGACTGTATGCGTTTGAATAAACTCGTCATCAAGCAATGAAGGCCGACCTgcggc                                                                                                                                   <  2:2011297/149‑1 (MQ=255)

                   gCCAGTTCGGCGATTTGTGTCTGACTTAGTCCAGAAATACGTTCGATATCTTTCCACTCGGAATTGAGAACGTCACGGCGTAGCTCGTCAAAGCCGACGGTATGCGTTTGAATAAACTCGTCATCAAGCAATGAAGGCCGACCTgcggc                                                                                                                                   >  1:3572524/1‑149 (MQ=255)

                       gTTCGGCGATTTGTGTCTGACTTAGTCCAGAAATACGTTCGATATCTTTCCACTCGGAATTGAGAACGTCACGGCGTAGCTCGTCAAAGCCGACGGTATGCGTTTGAATAAACTCGTCATCAAGCAATGAAGGCCGACCTGCGGCGCtt                                                                                                                               <  2:2632131/149‑1 (MQ=255)

                          cGGCGATTTGTGTCTGACTTAGTCCAGAAATACGTTCGATATCTTTCCACTCGGAATTGAGAACGTCACGGCGTAGCTCGTCAAAGCCGACGGTATGCGTTTGAATAAACTCGTCATCAAGCAATGAAGGCCGACCTGCGGCGCTTgca                                                                                                                            <  2:600206/149‑1 (MQ=255)

                          cGGCGATTTGTGTCTGACTTAGTCCAGAAATACGTTCGATATCTTTCCACTCGGAATTGAGAACGTCACGGCGTAGCTCGTCAAAGCCGACGGTATGCGTTTGAATAAACTCGTCATCAAGCAATGAAGGCCGACCTGCGGCGCTTgca                                                                                                                            <  2:1812828/149‑1 (MQ=255)

                                tttGTGTCTGTCTTAGTCCAGAAATACGTTCGATATCTTTCCACTCGGAATTGAGAACGTCACGGCGTAGCTCGTCAAAGCCGACGGTATGCGTTTGAATAAACTCGTCATCAAGCAATGAAGGCCGACCTGCGGCGCTTGCAGcatca                                                                                                                      <  2:433488/149‑1 (MQ=255)

                                tttGTGTCTGACTTAGTCCAGAAATACGTTCGATATCTTTCCACTCGGAATTGAGAACGTCACGGCGTAGCTCGTCAAAGCCGACGGTATGCGTTTGAATAAACTCGTCATCAAGCAATGAAGGCCGACCTGCGGCGCTTGCAGcatca                                                                                                                      >  1:2421335/1‑149 (MQ=255)

                                  tgtgTCTGACTTAGTCCAGAAATACGTTCGATATCTTTCCACTCGGAATTGAGAACGTCACGGCGTAGCTCGTCAAAGCCGACGGTATGCGTTTGAATAAACTCGTCATCAAGCAATGAAGGCCGACCTGCGGCGCTTGCAGCatcatc                                                                                                                    >  1:1504657/1‑149 (MQ=255)

                                     gtCTGACTTAGTCCAGAAATACGTTCGATATCTTTCCACTCGGAATTGAGAACGTCACGGCGTAGCTCGTCAAAGCCGACGGTATGCGTTTGAATAAACTCGTCATCAAGCAATGAAGGCCGACCTGCGGCGCTTGCAGCATCATcgcg                                                                                                                 >  2:3054991/1‑149 (MQ=255)

                                      tCTGACTTAGTCCAGAAATACGTTCGATATCTTTCCACTCGGAATTGAGAACGTCACGGCGTAGCTCGTCAAAGCCGACGGTATGCGTTTGAATAAACTCGTCATCAAGCAATGAAGGCCGACCTGCGGCGCTTGCAGCATCATCgcgc                                                                                                                >  1:2713040/1‑149 (MQ=255)

                                         gACTTAGTCCAGAAATACGTTCGATATCTTTCCACTCGGAATTGAGAACGTCACGGCGTAGCTCGTCAAAGCCGACGGTATGCGTTTGAATAAACTCGTCATCAAGCAATGAAGGCCGACCTGCGGCGCTTGCAGCATCATCGCGCTCa                                                                                                             >  2:2072311/1‑149 (MQ=255)

                                          aCTTAGTCCAGAAATACGTTCGATATCTTTCCACTCGGAATTGAGAACGTCACGGCGTAGCTCGTCAAAGCCGACGGTATGCGTTTGAATAAACTCGTCATCAAGCAATGAAGGCCGACCTGCGGCGCTTGCAGCATCATCGCGCTCaa                                                                                                            >  1:4444905/1‑149 (MQ=255)

                                            ttAGTCCAGAAATACGTTCGATATCTTTCCACTCGGAATTGAGAACGTCACGGCGTAGCTCGTCAAAGCCGACGGTATGCGTTTGAATAAACTCGTCATCAAGCAATGAAGGCCGACCTGCGGCGCTTGCAGCATCATCGCGCTCAAtt                                                                                                          >  1:3648505/1‑149 (MQ=255)

                                             tAGTCCAGAAATACGTTCGATATCTTTCCACTCGGAATTGAGAACGTCACGGCGTAGCTCGTCAAAGCCGACGGTATGCGTTTGAATAAACTCGTCATCAAGCAATGAAGGCCGACCTGCGGCGCTTGCAGCATCATCGCGCTCAATTa                                                                                                         >  2:4443490/1‑149 (MQ=255)

                                               gTCCAGAAATACGTTCGATATCTTTCCACTCGGAATTGAGAACGTCACGGCGTAGCTCGTCAAAGCCGACGGTATGCGTTTGAATAAACTCGTCATCAAGCAATGAAGGCCGACCTGCGGCGCTTGCAGCATCATCGCGCTCAATTAAc                                                                                                       >  2:2199950/1‑149 (MQ=255)

                                                tCCAGAAATACGTTCGATATCTTTCCACTCGGAATTGAGAACGTCACGGCGTAGCTCGTCAAAGCCGACGGTATGCGTTTGAATAAACTCGTCATCAAGCAATGAAGGCCGACCTGCGGCGCTTGCAGCATCATCGCGCTCAATTAACa                                                                                                      <  2:1565854/149‑1 (MQ=255)

                                                 ccAGAAATACGTTCGATATCTTTCCACTCGGAATTGAGAACGTCACGGCGTAGCTCGTCACAGCCGACGGTATGCGTTTGAATAAACTCGTCATCAAGCAATGAAGGCCGACCTGCGGCGCTTGCAGCATCATCGCGCTCAATTAACAg                                                                                                     >  2:3597684/1‑149 (MQ=255)

                                                  cAGAAATACGTTCGATATCTTTCCACTCGGAATTGAGAACGTCACGGCGTAGCTCGTCAAAGCCGACGGTATGCGTTTGAATAAACTCGTCATCAAGCAATGAAGGCCGACCTGCGGCGCTTGCAGCATCATCGCGCTCAATTAACAgg                                                                                                    <  1:3849180/149‑1 (MQ=255)

                                                   aGAAATACGTTCGATATCTTTCCACTCGGAATTGAGAACGTCACGGCGTAGCTCGTCAAAGCCGACGGTATGCGTTTGAATAAACTCGTCATCAAGCAATGAAGGCCGACCTGCGGCGCTTGCAGCATCATCGCGCTCAATTAACAGgc                                                                                                   >  2:2692171/1‑149 (MQ=255)

                                                    gAAATACGTTCGATATCTTTCCACTCGGAATTGAGAACGTCACGGCGTAGCTCGTCAAAGCCGACGGTATGCGTTTGAATAAACTCGTCATCAAGCAATGAAGGCCGACCTGCGGCGCTTGCAGCATCATCGCGCTCAATTAACAGgcg                                                                                                  >  1:631190/1‑149 (MQ=255)

                                                        tACGTTCGATATCTTTCCACTCGGAATTGAGAACGTCACGGCGTAGCTCGTCAAAGCCGACGGTATGCGTTTGAATAAACTCGTCATCAAGCAATGAAGGCCGACCTGCGGCGCTTGCAGCATCATCGCGCTCAATTAACAGGCGcatc                                                                                              >  1:45931/1‑149 (MQ=255)

                                                        tACGTTCGATATCTTTCCACTCGGAATTGAGAACGTCACGGCGTAGCTCGTCAAAGCCGACGGTATGCGTTTGAATAAACTCGTCATCAAGCAATGAAGGCCGACCTGCGGCGCTTGCAGCATCATCGCGCTCAATTAACAGGCGcatc                                                                                              >  2:493886/1‑149 (MQ=255)

                                                        tACGTTCGATATCTTTCCACTCGGAATTGAGAACGTCACGGCGTAGCTCGTCAAAGCCGACGGTATGCGTTTGAATAAACTCGTCATCAAGCAATGAAGGCCGACCTGCGGCGCTTGCAGCATCATCGCGCTCAATTAACAGGCGcatc                                                                                              >  1:73778/1‑149 (MQ=255)

                                                            ttCGATATCTTTCCACTCGGAATTGAGAACGTCACGGCGTAGCTCGTCAAAGCCGACGGTATGCGTTTGAATAAACTCGTCATCAAGCAATGAAGGCCGACCTGCGGCGCTTGCAGCATCATCGCGCTCAATTAACAGGCGCATCATcc                                                                                          >  1:2141944/1‑149 (MQ=255)

                                                            ttCGATATCTTTCCACTCGGAATTGAGAACGTCACGGCGTAGCTCGTCAAAGCCGACGGTATGCGTTTGAATAAACTCGTCATCAAGCAATGAAGGCCGACCTGCGGCGCTTGCAGCATCATCGCGCTCAATTAACAGGCGCATCATcc                                                                                          >  2:3271534/1‑149 (MQ=255)

                                                            ttCGATATCTTTCCACTCGGAATTGAGAACGTCACGGCGTAGCTCGTCAAAGCCGACGGTATGCGTTTGAATAAACTCGTCATCAAGCAATGAAGGCCGACCTGCGGCGCTTGCAGCATCATCGCGCTCAATTAACAGGCGCATCATcc                                                                                          >  1:3121871/1‑149 (MQ=255)

                                                                  atCTTTCCACTCGGAATTGAGAACGTCACGGCGTAGCTCGTCAAAGCCGACGGTATGCGTTTGAATAAACTCGTCATCAAGCAATGAAGGCCGACCTGCGGCGCTTGCAGCATCATCGCGCTCAATTAACAGGCGCATCATCCCCTTGa                                                                                    >  2:2089960/1‑149 (MQ=255)

                                                                      ttCCACTCGGAATTGAGAACGTCACGGCGTAGCTCGTCAAAGCCGACGGTATGCGTTTGAATAAACTCGTCATCAAGCAATGAAGGCCGACCTGCGGCGCTTGCAGCATCATCGCGCTCAATTAACAGGCGCATCATCCCCTTGAGCaa                                                                                >  1:546630/1‑149 (MQ=255)

                                                                      ttCCACTCGGAATTGAGAACGTCACGGCGTAGCTCGTCAAAGCCGACGGTATGCGTTTGAATAAACTCGTCATCAAGCAATGAAGGCCGACCTGCGGCGCTTGCAGCATCATCGCGCTCAATTAACAGGCGCATCATCCCCTTGAGCaa                                                                                <  2:3965471/149‑1 (MQ=255)

                                                                         cACTCGGAATTGAGAACGTCACGGCGTAGCTCGTCAAAGCCGACGGTATGCGTTTGAATAAACTCGTCATCAAGCAATGAAGGCCGACCTGCGGCGCTTGCAGCATCATCGCGCTCAATTAACAGGCGCATCATCCCCTTGAGCAACGc                                                                             <  2:803441/149‑1 (MQ=255)

                                                                         cACTCGGAATTGAGAACGTCACGGCGTAGCTCGTCAAAGCCGACGGTATGCGTTTGAATAAACTCGTCATCAAGCAATGAAGGCCGACCTGCGGCGCTTGCAGCATCATCGCGCTCAATTAACAGGCGCATCATCCCCTTGAGCAACGc                                                                             <  1:18388/149‑1 (MQ=255)

                                                                          aCTCGGAATTGAGAACGTCACGGCGTAGCTCGTCAAAGCCGACGGTATGCGTTTGAATAAACTCGTCATCAAGCTATGAAGGCCGACCTGCGGCGCTTGCAGCATCATCGCGCTCAATTAACAGGCGCATCATCCCCTTGAGCAACGcc                                                                            >  1:2901085/1‑149 (MQ=255)

                                                                           cTCGGAATTGAGAACGTCACGGCGTAGCTCGTCAAAGCCGACGGTATGCGTTTGAATAAACTCGTCATCAAGCAATGAAGGCCGACCTGCGGCGCTTGCAGCATCATCGCGCTCAATTAACAGGCGCATCATCCCCTTGAGCAACGCCa                                                                           <  2:2336311/149‑1 (MQ=255)

                                                                             cGGAATTGAGAACGTCACGGCGTAGCTCGTCAAAGCCGACGGTATGCGTTTGAATAAACTCGTCATCAAGCAATGAAGGCCGACCTGCGGCGCTTGCAGCATCATCGCGCTCAATTAACAGGCGCATCATCCCCTTGAGCAACGCCata                                                                         <  2:1668467/149‑1 (MQ=255)

                                                                             cGGAATTGAGAACGTCACGGCGTAGCTCGTCAAAGCCGACGGTATGCGTTTGAATAAACTCGTCATCAAGCAATGAAGGCCGACCTGCGGCGCTTGCAGCATCATCGCGCTCAATTAACAGGCGCATCATCCCCTTGAGCAACGCCata                                                                         <  2:1920406/149‑1 (MQ=255)

                                                                              ggAATTGAGAACGTCACGGCGTAGCTCGTCAAAGCCGACGGTATGCGTTTGAATAAACTCGTCATCAAGCAATGAAGGCCGACCTGCGGCGCTTGCAGCATCATCGCGCTCAATTAACAGGCGCATCATCCCCTTGAGCAACGCCatat                                                                        <  2:2561370/149‑1 (MQ=255)

                                                                                 aTTGAGAACGTCACGGCGTAGCTCGTCAAAGCCGACGGTATGCGTTTGAATAAACTCGTCATCAAGCAATGAAGGCCGACCTGCGGCGCTTGCAGCATCATCGCGCTCAATTAACAGGCGCATCATCCCCTTGAGCAACGCCATATCGc                                                                     >  1:4795436/1‑149 (MQ=255)

                                                                                 aTTGAGAACGTCACGGCGTAGCTCGTCAAAGCCGACGGTATGCGTTTGAATAAACTCGTCATCAAGCAATGAAGGCCGACCTGCGGCGCTTGCAGCATCATCGCGCTCAATTAACAGGCGCATCATCCCCTTGAGCAACGCCATATCGc                                                                     >  2:2285099/1‑149 (MQ=255)

                                                                                  ttGAGAACGTCACGGCGTAGCTCGTCAAAGCCGACGGTATGCGTTTGAATAAACTCGTCATCAAGCAATGAAGGCCGACCTGCGGCGCTTGCAGCATCATCGCGCTCAATTAACAGGCGCATCATCCCCTTGAGCAACGCCATATCGcc                                                                    <  1:5146901/149‑1 (MQ=255)

                                                                                     agaACGTCACGGCGTAGCTCGTCAAAGCCGACGGTATGCGTTTGAATAAACTCGTCATCAAGCAATGAAGGCCGACCTGCGGCGCTTGCAGCATCATCGCGCTCAATTAACAGGCGCATCATCCCCTTGAGCAACGCCATATCGccacc                                                                 >  1:1970864/1‑149 (MQ=255)

                                                                                     agaACGTCACGGCGTAGCTCGTCAAAGCCGACGGTATGCGTTTGAATAAACTCGTCATCAAGCAATGAAGGCCGACCTGCGGCGCTTGCAGCATCATCGCGCTCAATTAACAGGCGCATCATCCCCTTGAGCAACGCCATATCGccacc                                                                 >  1:145151/1‑149 (MQ=255)

                                                                                      gaACGTCACGGCGTAGCTCGTCAAAGCCGACGGTATGCGTTTGAATAAACTCGTCATCAAGCAATGAAGGCCGACCTGCGGCGCTTGCAGCATCATCGCGCTCAATTAACAGGCGCATCATCCCCTTGAGCAACGCCATATCGccacca                                                                >  1:2047138/1‑149 (MQ=255)

                                                                                       aaCGTCACGGCGTAGCTCGTCAAAGCCGACGGTATGCGTTTGAATAAACTCGTCATCAAGCAATGAAGGCCGACCTGCGGCGCTTGCAGCATCATCGCGCTCAATTAACAGGCGCATCATCCCCTTGAGCAACGCCATATCGCCACCaa                                                               <  1:4969259/149‑1 (MQ=255)

                                                                                       aaCGTCACGGCGTAGCTCGTCAAAGCCGACGGTATGCGTTTGAATAAACTCGTCATCAAGCAATGAAGGCCGACCTGCGGCGCTTGCAGCATCATCGCGCTCAATTAACAGGCGCATCATCCCCTTGAGCAACGCCATATCGCCACCaa                                                               >  1:2692620/1‑149 (MQ=255)

                                                                                               ggCGTAGCTCGTCAAAGCCGACGGTATGCGTTTGAATAAACTCGTCATCAAGCAATGAAGGCCGACCTGCGGCGCTTGCAGCATCATCGCGCTCAATTAACAGGCGCATCATCCCCTTGAGCAACGCCATATCGCCACCAATGCGCACg                                                       <  1:4259453/149‑1 (MQ=255)

                                                                                                gCGTAGCTCGTCAAAGCCGACGGTATGCGTTTGAATAAACTCGTCATCAAGCAATGAAGGCCGACCTGCGGCGCTTGCAGCATCATCGCGCGCAATTAACAGGCGCATCATCCCCTTGAGCAACGACATATCGCCACCAATGCGCACGt                                                      >  1:3167970/1‑149 (MQ=255)

                                                                                                 cGTAGCTCGTCAAAGCCGACGGTATGCGTTTGAATAAACTCGTCATCAAGCAATGAAGGCCGACCTGCGGCGCTTGCAGCATCATCGCGCTCAATTAACAGGCGCATCATCCCCTTGAGCAACGCCATATCGCCACCAATGCGCACGtt                                                     <  2:3447027/149‑1 (MQ=255)

                                                                                                  gTAGCTCGTCAAAGCCGACGGTATGCGTTTGAATAAACTCGTCATCAAGCAATGAAGGCCGACCTGCGGCGCTTGCAGCATCATCGCGCTCAATTAACAGGCGCATCATCCCCTTGAGCAACGCCATATCGCCACCAATGCGCACGTta                                                    <  1:2470043/149‑1 (MQ=255)

                                                                                                   tAGCTCGTCAAAGCCGACGGTATGCGTTTGAATAAACTCGTCATCAAGCAATGAAGGCCGACCTGCGGCGCTTGCAGCATCATCGCGCTCAATTAACAGGCGCATCATCCCCTTGAGCAACGCCATATCGCCACCAATGCGCACGTtat                                                   >  1:4048055/1‑149 (MQ=255)

                                                                                                    aGCTCGTCAAAGCCGACGGTATGCGTTTGAATAAACTCGTCATCAAGCAATGAAGGCCGACCTGCGGCGCTTGCAGCATCATCGCGCTCAATTAACAGGCGCATCATCCCCTTGAGCAACGCCATATCGCCACCAATGCGCACGTtata                                                  >  1:3559430/1‑149 (MQ=255)

                                                                                                        cGTCAAAGCCGACGGTATGCGTTTGAATAAACTCGTCATCAAGCTATGAAGGCCGACCTGCGGCGCTTGCAGCATCATCGCGCTCAATTAACAGGCGCATCATCCCCTTGAGCAACGCCATATCGCCACCAATGCGCACGTTAtagtag                                              <  2:1468891/149‑1 (MQ=255)

                                                                                                         gTCAAAGCCGACGGTATGCGTTTGAATAAACTCGTCATCAAGCAATGAAGGCCGACCTGCGGCGCTTGCAGCATCATCGCGCTCAATTAACAGGCGCATCATCCCCTTGAGCAACGCCATATCGCCACCAATGCGCACGTTATAGTAgg                                             >  1:2272385/1‑149 (MQ=255)

                                                                                                            aaaGCCGACGGTATGCGTTTGAATAAACTCGTCATCAAGCAATGAAGGCCGACCTGCGGCGCTTGCAGCATCATCGCGCTCAATTAACAGGCGCATCATCCCCTTGAGCAACGCCATATCGCCACCAATGCGCACGTTATAGTAGGCAc                                          >  1:4564259/1‑149 (MQ=255)

                                                                                                             aaGCCGACGGTATGCGTTTGAATAAACTCGTCATCAAGCAATGAAGGCCGACCTGCGGCGCTTGCAGCATCATCGCGCTCAATTAACAGGCGCATCATCCCCTTGAGCAACGCCATATCGCCACCAATGCGCACGTTATAGTAGGCACt                                         >  1:1095139/1‑149 (MQ=255)

                                                                                                             aaGCCGACGGTATGCGTTTGAATAAACTCGTCATCAAGCAATGAAGGCCGACCTGCGGCGCTTGCAGCATCATCGCGCTCAATTAACAGGCGCATCATCCCCTTGAGCAACGCCATATCGCCACCAATGCGCACGTTATAGTAGGCACt                                         >  1:2740406/1‑149 (MQ=255)

                                                                                                             aaGCCGACGGTATGCGTTTGAATAAACTCGTCATCAAGCAATGAAGGCCGACCTGCGGCGCTTGCAGCATCATCGCGCTCAATTAACAGGCGCATCATCCCCTTGAGCAACGCCATATCGCCACCAATGCGCACGTTATAGTAGGCACt                                         >  1:3021673/1‑149 (MQ=255)

                                                                                                              aGCCGACGGTATGCGTTTGAATAAACTCGTCATCAAGCAATGAAGGCCGACCTGCGGCGCTTGCAGCATCATCGCGCTCAATTAACAGGCGCATCATCCCCTTGAGCAACGCCATATCGCCACCAATGCGCACGTTATAGTAGGCACTg                                        <  2:2215727/149‑1 (MQ=255)

                                                                                                                 cGACGGTATGCGTTTGAATAAACTCGTCATCAAGCAATGAAGGCCGACCTGCGGCGCTTGCAGCATCATCGCGCTCAATTAACAGGCGCATCATCCCCTTGAGCAACGCCATATCGCCACCAATGCGCACGTTATAGTAGGCACTGGcc                                     <  1:3973522/149‑1 (MQ=255)

                                                                                                                 cGACGGTATGCGTTTGAATAAACTCGTCATCAAGCAATGAAGGCCGACCTGCGGCGCTTGCAGCATCATCGCGCTCAATTAACAGGCGCATCATCCCCTTGAGCAACGCCATATCGCCACCAATGCGCACGTTATAGTAGGCACTGGcc                                     <  2:2013205/149‑1 (MQ=255)

                                                                                                                  gACGGTATGCGTTTGAATAAACTCGTCATCAAGCAATGAAGGCCGACCTGCGGCGCTTGCAGCATCATCGCGCTCAATTAACAGGCGCATCATCCCCTTGAGCAACGCCATATCGCCACCAATGCGCACGTTATAGTAGGCACTGGCCa                                    >  2:1373427/1‑149 (MQ=255)

                                                                                                                     ggTATGCGTTTGAATAAACTCGTCATCAAGCAATGAAGGCCGACCTGCGGCGCTTGCAGCATCATCGCGCTCAATTAACAGGCGCATCATCCCCATGAGCAACGCCATATCGCCACCAATGCGCACGTTATAGTAGGCACTGGCCAACt                                 >  2:1076605/1‑149 (MQ=255)

                                                                                                                      gTATGCGTTTGAATAAACTCGTCATCAAGCAATGAAGGCCGACCTGCGGCGCTTGCAGCATCATCGCGCTCAATTAACAGGCGCATCATCCCCTTGAGCAACGCCATATCGCCACCAATGCGCACGTTATAGTAGGCACTGGCCAACTg                                <  1:1010399/149‑1 (MQ=255)

                                                                                                                       tATGCGTTTGAATAAACTCGTCATCAAGCAATGAAGGCCGACCTGCGGCGCTTGCAGCATCATCGCGCTCAATTAACAGGCGCATCATCCCCTTGAGCAACGCCATATCGCCACCAATGCGCACGTTATAGTAGGCACTGGCCAACTGa                               >  1:4821358/1‑149 (MQ=255)

                                                                                                                       tATGCGTTTGAATAAACTCGTCATCAAGCAATGAAGGCCGACCTGCGGCGCTTGCAGCATCATCGCGCTCAATTAACAGGCGCATCATCCCCTTGAGCAACGCCATATCGCCACCAATGCGCACGTTATAGTAGGCACTCGCCAACTGa                               >  2:1243847/1‑149 (MQ=255)

                                                                                                                         tGCGTTTGAATAAAATCGACATCAAGCAATGAAGGCCGACATGCGGCGCTTGCAGCATCATCGCGCTCAATTAACAGGCGCATCATCCACTTGAGCAACCCCAAATCGCCACCAATGCGCACGTTATAGTAGGCACTGGCCAACTGAGt                             >  2:50476/1‑149 (MQ=255)

                                                                                                                          gCGTTTGAATAAACTCGTCATCAAGCTATGAAGGCCGACCTGCGGCGCTTGCAGCATCATCGCGCTCAATTAACAGGCGCATCATCCCCTTGAGCAACGCCATATCGCCACCAATGCGCACGTTATAGTAGGCACTGGCCAACTGAGtc                            <  2:2810282/149‑1 (MQ=255)

                                                                                                                           cGTTTGAATAAACTCGTCATCAAGCAATGAAGGCCGACCTGCGGCGCTTGCAGCATCATCGCGCTCAATTAACAGGCGCATCATCCCCTTGAGCAACGCCATATCGCCACCAATGCGCACGTTATAGTAGGCACTGGCCAACTGAGtct                           <  1:4455000/149‑1 (MQ=255)

                                                                                                                            gTTTGAATAAACTCGTCATCAAGCAATGAAGGCCGACCTGCGGCGCTTGCAGCATCATCGCGCTCAATTAACAGGCGCATCATCCCCTTGAGCAACGCCATATCGCCACCAATGCGCACGTTATAGTAGGCACTGGCCAACTGAGtctc                          <  1:1712479/149‑1 (MQ=255)

                                                                                                                             tttGAATAAACTCGTCATCAAGCAATGAAGGCCGACCTGCGGCGCTTGCAGCATCATCGCGCTCAATTAACAGGCGCATCATCCCCTTGAGCAACGCCATATCGCCACCAATGCGCACGTTATAGTAGGCACTGGCCAACTGAGTCTCa                         >  1:3936533/1‑149 (MQ=255)

                                                                                                                               tGAATAAACTCGTCATCAAGCAATGAAGGCCGACCTGCGGCGCTTGCAGCATCATCGCGCTCAATTAACAGGCGCATCATCCCCTTGAGCAACGCCATATCGCCACCAATGCGCACGTTATAGTAGGCACTGGCCAACTGAGTCTCaga                       <  1:5015386/149‑1 (MQ=255)

                                                                                                                                gAATAAACTCGTCATCAAGCAATGAAGGCCGACCTGCGGCGCTTGCAGCATCATCGCGCTCAATTAACAGGCGCATCATCCCCTTGAGCAACGCCATATCGCCACCAATGCGCACGTTATAGTAGGCACTGGCCAACTGAGTCTCagag                      >  2:4665721/1‑149 (MQ=255)

                                                                                                                                   tAAACTCGTCATCAAGCAATGAAGGCCGACCTGCGGCGCTTGCAGCATCATCGCGCTCAATTAACAGGCGCATCATCCCCTTGAGCAACGCCATATCGCCACCAATGCGCACGTTATAGTAGGCACTGGCCAACTGAGTCTCAGAGTTc                   <  2:4143346/149‑1 (MQ=255)

                                                                                                                                     aaCTCGTCATCAAGCAATGAAGGCCGACCTGCGGCGCTTGCAGCATCATCGCGCTCAATTAACAGGCGCATCATCCCCTTGAGCAACGCCATATCGCCACCAATGCGCACGTTATAGTAGGCACTGGCCAACTGAGTCTCAGAGTTCGt                 <  1:1686133/149‑1 (MQ=255)

                                                                                                                                     aaCTCGTCATCAAGCAATGAAGGCCGACCTGCGGCGCTTGCAGCATCATCGCGCTCAATTAACAGGCGCATCATCCCCTTGAGCAACGCCATATCGCCACCAATGCGCACGTTATAGTAGGCACTGGCCAACTGAGTCTCAGAGTTCGt                 >  2:4157255/1‑149 (MQ=255)

                                                                                                                                       cTCGTCATCAAGCAATGAAGGCCGACCTGCGGCGCTTGCAGCATCATCGCGCTCAATTAACAGGCGCATCATCCCCTTGAGCAACGCCATATCGCCACCAATGCGCACGTTATAGTAGGCACTGGCCAACTGAGTCTCAGAGTTCGTCa               <  2:1629973/149‑1 (MQ=255)

                                                                                                                                       cTCGTCATCAAGCAATGAAGGCCGACCTGCGGCGCTTGCAGCATCATCGCGCTCAATTAACAGGCGCATCATCCCCTTGAGCAACGCCATATCGCCACCAATGCGCACGTTATAGTAGGCACTGGCCAACTGAGTCTCAGAGTTCGTCa               <  2:4540105/149‑1 (MQ=255)

                                                                                                                                        tCGTCATCAAGCTATGAAGGCCGACCTGCGGCGCTTGCAGCATCATCGCGCTCAATTAACAGGCGCATCATCCCCTTGAGCAACGCCATATCGCCACCAATGCGCACGTTATAGTAGGCACTGGCCAACTGAGTCTCAGAGTTCGTCAg              >  1:2816457/1‑149 (MQ=255)

                                                                                                                                         cGTCATCAAGCAATGAAGGCCGACCTGCGGCGCTTGCAGCATCATCGCGCTCAATTAACAGGCGCATCATCCCCTTGAGCAACGCCATATCGCCACCAATGCGCACGTTATAGTAGGCACTGGCCAACTGAGTCTCAGAGTTCGTCAGc             <  2:3168030/149‑1 (MQ=255)

                                                                                                                                          gTCATCAAGCAATGAAGGCCGACCTGCGGCGCTTGCAGCATCATCGCGCTCAATTAACAGGCGCATCATCCCCTTGAGCAACGCCATATCGCCACCAATGCGCACGTTATAGTAGGCACTGGCCAACTGAGTCTCAGAGTTCGTCAGCa            <  2:5135599/149‑1 (MQ=255)

                                                                                                                                           tcatcaAGCAATGAAGGCCGACCTGCGGCGCTTGCAGCATCATCGCGCTCAATTAACAGGCGCATCATCCCCTTGAGCAACGCCATATCGCCACCAATGCGCACGTTATAGTAGGCACTGGCCAACTGAGTCTCAGAGTTCGTCAGCAt           <  2:90931/149‑1 (MQ=255)

                                                                                                                                            catcaAGCAATGAAGGCCGACCTGCGGCGCTTGCAGCATCATCGCGCTCAATTAACAGGCGCATCATCCCCTTGAGCAACGCCATATCGCCACCAATGCGCACGTTATAGTAGGCACTGGCCAACTGAGTCTCAGAGTTCGTCAGCAtt          <  1:3271534/149‑1 (MQ=255)

                                                                                                                                             atcaAGCAATGAAGGCCGACCTGCGGCGCTTGCAGCATCATCGCGCTCAATTAACAGGCGCATCATCCCCTTGAGCAACGCCATATCGCCACCAATGCGCACGTTATAGTAGGCACTGGCCAACTGAGTCTCAGAGTTCGTCAGCAttt         >  2:18623/1‑149 (MQ=255)

                                                                                                                                             atcaAGCAATGAAGGCCGACCTGCGGCGCTTGCAGCATCATCGCGCTCAATTAACAGGCGCATCATCCCCTTGAGCAACGCCATATCGCCACCAATGCGCACGTTATAGTAGGCACTGGCCAACTGAGTCTCAGAGTTCGTCAGCAttt         <  2:3535824/149‑1 (MQ=255)

                                                                                                                                              tcaAGCAATGAAGGCCGACCTGCGGCGCTTGCAGCATCATCGCGCTCAATTAACAGGCGCATCATCCCCTTGAGCAACGCCATATCGCCACCAATGCGCACGTTATAGTAGGCACTGGCCAACTGAGTCTCAGAGTTCGTCAGCATTTc        >  2:4547768/1‑149 (MQ=255)

                                                                                                                                              tcaAGCAATGAAGGCCGACCTGCGGCGCTTGCAGCATCATCGCGCTCAATTAACAGGCGCATCATCCCCTTGAGCAACGCCATATCGCCACCAATGCGCACGTTATAGTAGGCACTGGCCAACTGAGTCTCAGAGTTCGTCAGCATTTc        <  1:3427698/149‑1 (MQ=255)

                                                                                                                                              tcaAGCAATGAAGGCCGACCTGCGGCGCTTGCAGCATCATCGCGCTCAATTAACAGGCGCATCATCCCCTTGAGCAACGCCATATCGCCACCAATGCGCACGTTATAGTAGGCACTGGCCAACTGAGTCTCAGAGTTCGTCAGCATTTc        >  1:1213730/1‑149 (MQ=255)

                                                                                                                                                aaGCAATGAAGGCCGACCTGCGGCGCTTGCAGCATCATCGCGCTCAATTAACAGGCGCATCATCCCCTTGAGCAACGCCATATCGCCACCAATGCGCACGTTATAGTAGGCACTGGCCAACTGAGTCTCAGAGTTCGTCAGCATTTCaa      >  1:4153805/1‑149 (MQ=255)

                                                                                                                                                aaGCAATGAAGGCCGACCTGCGGCGCTTGCAGCATCATCGCGCTCAATTAACAGGCGCATCATCCCCTTGAGCAACGCCATATCGCCACCAATGCGCACGTTATAGTAGGCACTGGCCAACTGAGTCTCAGAGTTCGTCAGCATTTCaa      >  2:2438113/1‑149 (MQ=255)

                                                                                                                                                  gCAATGAAGGCCGACCTGCGGCGCTTGCAGCATCATCGCGCTCAATTAACAGGCGCATCATCCCCTTGAGCAACGCCATATCGCCACCAATGCGCACGTTATAGTAGGCACTGGCCAACTGAGTCTCAGAGTTCGTCAGCATTTCAAAc    >  1:3319750/1‑149 (MQ=255)

GGCAGCGGCATATGCGTCAGCCAGTTCGGCGATTTGTGTCTGACTTAGTCCAGAAATACGTTCGATATCTTTCCACTCGGAATTGAGAACGTCACGGCGTAGCTCGTCAAAGCCGACGGTATGCGTTTGAATAAACTCGTCATCAAGCAATGAAGGCCGACCTGCGGCGCTTGCAGCATCATCGCGCTCAATTAACAGGCGCATCATCCCCTTGAGCAACGCCATATCGCCACCAATGCGCACGTTATAGTAGGCACTGGCCAACTGAGTCTCAGAGTTCGTCAGCATTTCAAACGG  >  CP009273/1579670‑1579966

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`