| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 1,673,380 | T→A | 7.7% | S233T (TCA→ACA) | ydgH → | DUF1471 family periplasmic protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 1,673,380 | 0 | T | A | 7.7% | 706.0 / 4.6 | 246 | S233T (TCA→ACA) | ydgH | DUF1471 family periplasmic protein |
| Reads supporting (aligned to +/- strand): ref base T (106/116); new base A (8/15); total (114/132) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.77e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 267 total aligned reads displayed.
GAGCCCGGATGTGATCCCGGCAGATTCCGAAGCAGGACGTGCAGCTCTGGCTGCCGGTGGCGAAGCCGCGAAGAAAGTTGAGATCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTGAAACCCAGTCATCAAAAGGCGGGCGTTACACCGTCACGCTCCCGGATGGCACTAAAGTCGAAGAACTGAACAAAGCGACCGCAGCGATGATGGTCCCGTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTATCAGGTTGC > CP009273/1673232‑1673528 | gagCCCGGATGTGATCCCGGCAGATTCCGAAGCAGGACGTGCAGCTCTGGCTGCCGGTGGCGAAGCCGCGAAGAAAGTTGAGATCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTGAAACCCAGtcat > 1:298193/1‑149 (MQ=255) cGGATGTGATCCCGGCAGATTCCGAAGCAGGACGTGCAGCTCTGGCTGCCGGTGGCGAAGCCGCGAAGAAAGTTGAGATCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTGAAACCCAGTCATCaaaa < 1:1375640/149‑1 (MQ=255) ggATGTGATCCCGGCAGATTCCGAAGCAGGACGTGCAGCTCTGGCTGCCGGTGGCGAAGCCGCGAAGAAAGTTGAGATCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTGAAACCCAGTCATCAAAAg < 1:523616/149‑1 (MQ=255) gATGTGATCCCGGCAGATTCCGAAGCAGGACGTGCAGCTCTGGCTGCCGGTGGCGAAGCCGCGAAGAAAGTTGAGATCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTGAAACCCAGTCATCAAAAgg > 1:1237667/1‑149 (MQ=255) aTGTGATCCCGGCAGATTCCGAAGCAGGACGTGCAGCTCTGGCTGCCGGTGGCGAAGCCGCGAAGAAAGTTGAGATCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTGAAACCCAGTCATCAAAAggc < 1:2521574/149‑1 (MQ=255) gtgATCCCGGCAGATTCCGAAGCAGGACGTGCAGCTCTGGCTGCCGGTGGCGAAGCCGCGAAGAAAGTTGAGATCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTGAAACCCAGTCATCAAAAggcgg > 2:3219038/1‑149 (MQ=255) tgATCCCGGCAGATTCCGAAGCAGGACGTGCAGCTCTGGCTGCCGGTGGCGAAGCCGCGAAGAAAGTTGAGATCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTGAAACCCAGTCATCAAAAggcggg < 1:4771455/149‑1 (MQ=255) tCCCGGCAGATTCCGAAGCAGGACGTGCAGCTCGGGCTGCCGGTGGCGAAGCCGCGAAGAAAGTGGAGATCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGGCGGTCGCTTCTTTGAAACCCAGTCATCAAAAGGCGGGCGt < 2:1833838/149‑1 (MQ=255) ccGGCAGATTCCGAAGCAGGACGTGCAGCTCTGGCTGCCGGTGGCGAAGCCGCGAAGAAAGTTGAGATCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTGAAACCCAGTCATCAAAAGGCGGGCGTTa > 2:2253554/1‑149 (MQ=255) ggCAGATTCCGAAGCAGGACGTGCAGCTCTGGCTGCCGGTGGCGAAGCCGCGAAGAAAGTTGAGATCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTGAAACCCAGTCATCAAAAGGCGGGCGTTaca < 2:4992747/149‑1 (MQ=255) ggCAGATTCCGAAGCAGGACGTGCAGCTCTGGCTGCCGGTGGCGAAGCCGCGAAGAAAGTTGAGATCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTGAAACCCAGTCATCAAAAGGCGGGCGTTaca < 1:3089948/149‑1 (MQ=255) ggCAGATTCCGAAGCAGGACGTGCAGCTCTGGCTGCCGGTGGCGAAGCCGCGAAGAAAGTTGAGATCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTGAAACCCAGTCATCAAAAGGCGGGCGTTaca < 1:85247/149‑1 (MQ=255) gCAGATTCCGAAGCAGGACGTGCAGCTCTGGCTGCCGGTGGCGAAGCCGCGAAGAAAGTTGAGATCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTGAAACCCAGTCATCAAAAGGCGGGCGTTacac < 1:1292972/149‑1 (MQ=255) gATTCCGAAGCAGGACGTGCAGCTCTGGCTGCCGGTGGCGAAGCCGCGAAGAAAGTTGAGATCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTGTAACCCAGTCATCAAAAGGCGGGCGTTACACCGt > 1:336/1‑149 (MQ=255) gATTCCGAAGCAGGACGTGCAGCTCTGGCTGCCGGTGGCGAAGCCGCGAAGAAAGTTGAGATCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTGAAACCCAGTCATCAAAAGGCGGGCGTTACACCGt > 1:1633836/1‑149 (MQ=255) gATTCCGAAGCAGGACGTGCAGCTCTGGCTGCCGGTGGCGAAGCCGCGAAGAAAGTTGAGATCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTGAAACCCAGTCATCAAAAGGCGGGCGTTACACCGt > 1:4250702/1‑149 (MQ=255) gATTCCGAAGCAGGACGTGCAGCTCTGGCTGCCGGTGGCGAAGCCGCGAAGAAAGTTGAGATCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTATTTGAAACCCAGTCAACAAAAGGCGGGCGTTACACCGt > 1:299746/1‑149 (MQ=255) aTTCCGAAGCAGGACGTGCAGCTCTGGCTGCCGGTGGCGAAGCCGCGAAGAAAGTTGAGATCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTGAAACCCAGTCATCAAAAGGCGGGCGTTACACCGTc > 2:2686679/1‑149 (MQ=255) tCCGAAGCAGGACGTGCAGCTCTGGCTGCCGGTGGCGAAGCCGCGAAGAAAGTTGAGATCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTGAAACCCAGTCATCAAAAGGCGGGCGTTACACCGTCAc < 2:2605372/149‑1 (MQ=255) gAAGCAGGACGTGCAGCTCTGGCTGCCGGTGGCGAAGCCGCGAAGAAAGTTGAGATCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTGAAACCCAGTCATCAAAAGGCGGGCGTTACACCGTCACGCt < 2:3620333/149‑1 (MQ=255) aGCAGGACGTGCAGCTCTGGCTGCCGGTGGCGAAGCCGCGAAGAAAGTTGAGATCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTGAAACCCAGTCATCAAAAGGCGGGCGTTACACCGTCACGCTcc < 2:405926/149‑1 (MQ=255) gCAGGACGTGCAGCTCTGGCTGCCGGTGGCGAAGCCGCGAAGAAAGTTGAGATCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTGAAACCCAGTCATCAAAAGGCGGGCGTTACACCGTCACGCTccc > 2:4931754/1‑149 (MQ=255) gCAGGACGTGCAGCTCTGGCTGCCGGTGGCGAAGCCGCGAAGAAAGTTGAGATCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTGAAACCCAGTCATCAAAAGGCGGGCGTTACACCGTCACGCTccc < 1:4219902/149‑1 (MQ=255) aGGACGTGCAGCTCTGGCTGCCGGTGGCGAAGCCGCGAAGAAAGTTGAGATCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTGAAACCCAGTCATCAAAAGGCGGGCGTTACACCGTCACGCTCCCgg < 1:1510293/149‑1 (MQ=255) ggACGTGCAGCTCGGGCTGCCGGTGGCGAAGCCGCGAAGAAAGTTGAGATCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTGAAACCCAGTCATCAAAAGGCGGGCGTTACACCGTCACGCTCCCGGa < 2:3549521/149‑1 (MQ=255) aCGTGCAGCTCTGGCTGCCGGTGGCGAAGCCGCGAAGAAAGTTGAGATCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTGAAACCCAGTCATCAAAAGGCGGGCGTTACACCGTCACGCTCCCGGATg < 1:3815296/149‑1 (MQ=255) gTGCAGCTCTGGCTGCCGGTGGCGAAGCCGCGAAGAAAGTTGAGATCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTGAAACCCAGTCAACAAAAGGCGGGCGTTACACCGGCACGCGCCCGGATGGc > 1:4006401/1‑149 (MQ=255) tGCAGCTCTGGCTGCCGGTGGCGAAGCCGCGAAGAAAGTTGAGATCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTGAAACCCAGTCATCAAAAGGCGGGCGTTACACCGTCACGCTCCCGGATGGCa < 2:90964/149‑1 (MQ=255) tGCAGCTCTGGCTGCCGGTGGCGAAGCCGCGAAGAAAGTTGAGATCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTGAAACCCAGTCATCAAAAGGCGGGCGTTACACCGTCACGCTCCCGGATGGCa < 2:2718635/149‑1 (MQ=255) tGCAGCTCTGGCTGCCGGTGGCGAAGCCGCGAAGAAAGTTGAGATCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTGAAACCCAGTCATCAAAAGGCGGGCGTTACACCGTCACGCTCCCGGATGGCa < 1:3269171/149‑1 (MQ=255) gCAGCTCTGGCTGCCGGTGGCGAAGCCGCGAAGAAAGTTGAGATCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTGAAACCCAGTCATCAAAAGGCGGGCGTTACACCGTCACGCTCCCGGATGGCAc < 2:4079062/149‑1 (MQ=255) gCAGCTCTGGCTGCCGGTGGCGAAGCCGCGAAGAAAGTTGAGATCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTGAAACCCAGTCATCAAAAGGCGGGCGTTACACCGTCACGCTCCCGGATGGCAc < 1:4699443/149‑1 (MQ=255) gCTCTGGCTGCCGGTGGCGAAGCCGCGAAGAAAGTTGAGATCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCGTCTTTGTAACCCAGTCATCAAAAGGCGGGCGTTACACCGTCACGCTCCCGGATGGCACTaa > 1:2196174/1‑149 (MQ=255) tctGGCTGCCGGTGGCGAAGCCGCGAAGAAAGTTGAGATCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTGAAACCCAGTCATCAAAAGGCGGGCGTTACACCGTCACGCTCCCGGATGGCACTAAAg < 1:1056051/149‑1 (MQ=255) ctGGCTGCCGGTGGCGAAGCCGCGAAGAAAGTTGAGATCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTGAAACCCAGTCATCAAAAGGCGGGCGTTACACCGTCACGCTCCCGGATGGCACTAAAGt < 2:1437534/149‑1 (MQ=255) ctGGCTGCCGGTGGCGAAGCCGCGAAGAAAGTTGAGATCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTGAAACCCAGTCATCAAAAGGCGGGCGTTACACCGTCACGCTCCCGGATGGCACTAAAGt < 2:1437427/149‑1 (MQ=255) tGGCTGCCGGTGGCGAAGCCGCGAAGAAAGTTGAGATCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTGAAACCCAGTCATCAAAAGGCGGGCGTTACACCGTCACGCTCCCGGATGGCACTAAAGTc < 2:4678371/149‑1 (MQ=255) ggCTGCCGGTGGCGAAGCCGCGAAGAAAGTTGAGATCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTGAAACCCAGTCATCAAAAGGCGGGCGTTACACCGTCACGCTCCCGGATGGCACTAAAGTCg > 2:3674566/1‑149 (MQ=255) tGCCGGTGGCGAAGCCGCGAAGAAAGTTGAGATCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTGAAACCCAGTCATCAAAAGGCGGGCGTTACACCGTCACGCTCCCGGATGGCACTAAAGTCgaag < 2:4491678/149‑1 (MQ=255) gCCGGTGGCGAAGCCGCGAAGAAAGTTGAGATCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTGAAACCCAGTCATCAAAAGGCGGGCGTTACACCGTCACGCTCCCGGATGGCACTAAAGTCgaaga < 2:4196620/149‑1 (MQ=255) cGGTGGCGAAGCCGCGAAGAAAGTTGAGATCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTGAAACCCAGTCAACAAAAGGCGGGCGTTACACCGTCACGCTCCCGGATGGCACTAAAGTCGAAGAAc > 1:1844625/1‑149 (MQ=255) tGGCGAAGCCGCGAAGAAAGTTGAGATCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTGAAACCCAGTCATCAAAAGGCGGGCGTTACACCGTCACGCTCCCGGATGGCACTAAAGTCGAAGAACTGa < 2:5228225/149‑1 (MQ=255) ggCGAAGCCGCGAAGAAAGTTGAGATCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTGAAACCCAGTCATCAAAAGGCGGGCGTTACACCGTCACGCTCCCGGATGGCACTAAAGTCGAAGAACTGaa < 2:4627844/149‑1 (MQ=255) aaGCCGCGAAGAAAGTTGAGATCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTGAAACCCAGTCATCAAAAGGCGGGCGTTACACCGTCACGCTCCCGGATGGCACTAAAGTCGAAGAACTGAACaaa > 2:1235491/1‑149 (MQ=255) aaGCCGCGAAGAAAGTTGAGATCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTGAAACCCAGTCATCAAAAGGCGGGCGTTACACCGTCACGCTCCCGGATGGCACTAAAGTCGAAGAACTGAACaaa < 2:4753946/149‑1 (MQ=255) aaGCCGCGAAGAAAGTTGAGATCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTGAAACCCAGTCATCAAAAGGCGGGCGTTACACCGTCACGCTCCCGGATGGCACTAAAGTCGAAGAACTGAACaaa < 2:699789/149‑1 (MQ=255) gCCGCGAAGAAAGTTGAGATCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTGAAACCCAGTCATCAAAAGGCGGGCGTTACACCGTCACGCTCCCGGATGGCACTAAAGTCGAAGAACTGAACAAAGc > 2:2502162/1‑149 (MQ=255) gCCGCGAAGAAAGTTGAGATCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTGAAACCCAGTCATCAAAAGGCGGGCGTTACACCGTCACGCTCCCGGATGGCACTAAAGTCGAAGAACTGAACAAAGc > 2:868532/1‑149 (MQ=255) cgcgAAGAAAGTTGAGATCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTGAAACCCAGTCATCAAAAGGCGGGCGTTACACCGTCACGCTCCCGGATGGCACTAAAGTCGAAGAACTGAACAAAGCGa < 2:20880/149‑1 (MQ=255) cgcgAAGAAAGTTGAGATCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTGAAACCCAGTCATCAAAAGGCGGGCGTTACACCGTCACGCTCCCGGATGGCACTAAAGTCGAAGAACTGAACAAAGCGa < 2:3452932/149‑1 (MQ=255) gcgAAGAAAGTTGAGATCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTGAAACCCAGTCATCAAAAGGCGGGCGTTACACCGTCACGCTCCCGGATGGCACTAAAGTCGAAGAACTGAACAAAGCGAc < 2:1307343/149‑1 (MQ=255) gTTGAGATCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTGAAACCCAGTCATCAAAAGGCGGGCGTTACACCGTCACGCTCCCGGATGGCACTAAAGTCGAAGAACTGAACAAAGCGACCGCAGCgat > 2:423670/1‑149 (MQ=255) ttGAGATCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTGAAACCCAGTCATCAAAAGGCGGGCGTTACACCGTCACGCTCCCGGATGGCACTAAAGTCGAAGAACTGAACAAAGCGACCGCAGCgatg > 2:4560176/1‑149 (MQ=255) tGAGATCCCGGGTGTTGCTACTACCGAATCACAAAGTTCTGAAGTCGGTCGCTTATTTTAAACCCAGTAAAAAAAAGGCGGGCTTTACACCGTCACGCTCCCGGATGGAAATAAAGTCGAATAACTGAACAAAGCGACCGCAGCgatga < 2:2985723/149‑1 (MQ=255) gaTCCGGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTGAAACCCAGTCATCAAATGGCGGGCGTTACACCGTCACGCTCCCGGATGGCACTAAAGTCGAAGAACTGAACAAAGCGACCGCAGCGATGATgg < 2:3876765/149‑1 (MQ=255) gaTCCCGGGTGTTGCGCCTACCGCATCACCAAGTTCTGAAGTCGGTCGCGTCGTGGAACCCCGGTCCTCCACAGGCGGGCGGTTCCCCGTCACGCGCCCGGGTGGCACGACGGTCGGCGACCTGCACCACGCGACCGCGGCGCTGATgg > 2:2019434/1‑149 (MQ=255) gaTCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGACGTCGGTCGCTTCTTTGAAACCCCGTCATCAAAAGGCGGGCGTTACACCGTCACGCTCCCGGATGGCACTAAAGTCGAATAACTGAACAAAGCGACCGCAGCGATGATgg < 2:3409110/149‑1 (MQ=255) tCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTGAAACCCAGTCATCAAAAGGCGGGCGTTACACCGTCACGCTCCCGGATGGCACTAAAGTCGAAGAACTGAACAAAGCGACCGCAGCGATGATGGTc > 2:2335041/1‑149 (MQ=255) tCCCGGGGGTGGCGACTACCGCATCACCAAGGTCTGAAGTCGGTCGCTTCTTTGAAACCCACTCATCAAAAGGCGGGCGTTACACCGTCACGCGCCCGGATGGCACGAAAGTCGAAGAACTGAACAAACCGACCGCAGCCATGAAGGTc > 2:3247900/1‑149 (MQ=255) ccGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTGAAACCCAGTCATAAAAAGGCGGGCGTTACACCGTCACGCTCCCGGATGGCACTAAAGTCGAAGAACTGAACAAAGCGACCGCAGCGATGATGGTccc > 2:3300152/1‑149 (MQ=255) gtgtTGCTCCAACCGCATCACCAAGTTCTGACGTGGGTGGCTTCTTTTAAACCCCGACATCAAAGGGCGGGCGTTACACTGTCACCCTCCGGGTTGGCACTAAAGACGACCAACTGAACAAAGCGACCGCAGCGATGATGGTCCCGTTc < 2:4859103/149‑1 (MQ=255) gtgtTGCGACTACCGCATCACCAAGTTCTGACGTCGGTCGCTTCTTTGAAACCCAGTCATCAAAAGGCGGGCGTTACACCGTCACGCTCCCGGATGGCACTAAAGTCGAAGAACTGAACAAAGCGACCGCAGCGATGATGGTCCCGTTc < 2:5194512/149‑1 (MQ=255) gtgtTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTTAAACCCCGTCATCAAAATGCGGGCGTTTCACCGTCACGCTCCCGGATGGCACTAAAGTCGAAGAACTGAACAAAGCGACCGCAGCGATGATGGTCCCGTTc > 1:977648/1‑149 (MQ=255) ttGCGACTACCCAAACACAATGTTCTGCAGTCGGTCGCTTCTTTTAAACCCAGAAAACAAATGGGGGGCTTAACCCCGCCACGCTCCCGGTTGGCACTACCGTCGAATAACTTAACAAAGCGACCGCAGCGATGATGGTCCCGTTCGAc < 1:3785561/149‑1 (MQ=255) ggCGACTCCCGCATCACCAATTTCTGAAGTCGGTCGCTTCTTTGAAACCCCGGCAACAAATGGCGGGCGTAACACCGTCACGCTCCGGGATGGAACAAACGTCGAACAACTTAACAACGCGACCGCAGCGATGATGGTCCCGTTCGACa < 2:1810447/148‑1 (MQ=255) tACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTGAAACCCAGTCATCAAAATGCGGGCGTTACACCGTCACGCTCCCGGATGGCACTAAAGTCGAAGAACTGAACAAAGCGACCGCAGCGATGATGGTCCCGTTCGACAGCATCa > 2:43942/1‑149 (MQ=255) ccGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTGAAACCCAGTCATCAAATGGCGGGCGTTACACCGTCACGCTCCCGGATGGCACTAAAGTCGACGAACTGAACAACGCGACCGCAGCGATGATGGTCCCGTTCGACAGCATCaaa < 2:393177/149‑1 (MQ=255) aTCACCAAGTTCTGAAGTCGGTCGCTTATTTTAAACCCAGTCATAAAAAGGCGGGCGTTACAACGTCACGCACCCGGAAGGCACTAAATTCGAATAACTGAACAAAGCGACCGCACCGATGATGGTACCGTTAGACAGCAACAAAtaat > 2:2335181/1‑146 (MQ=255) tCACAATGTTCTGATGTCGGGCGCTTATTTGAAACCCAGTCATCAAAAGGCGGGCGTTACACCGTCACGCTCCCGGTTGGAACTAACGTCGAAGAACTGAACAAAGCGACCGCAGCGATGATGGTCCCGTTCGACAGCATCAAATtctc < 1:4707587/149‑1 (MQ=255) cacaAAGTTCTGAAGTCGGTCGCTTATTTTAAACCCAGTCATAAAATGGCGGGCGTTACACCGTCACGCTCCGGGTTGGAACTAATGTCGAAGAACTTAACAAAGCGACCGCAGCGATGATGGTCCCGTTCGACAGCATCAAATTctct < 1:799752/149‑1 (MQ=255) cACCATGTTCTGAAGTCGGTCGCTTCTTTGAAACCCAGTCATCAAAAGGCGGGCGTTACACCGTCACGCTCCCGGATGGCAATAAAGTCGAAGAACTGAACAACGCGACCGCAGCGATGATGGTCCCGTTCGACAGCATCAAATTctct < 2:1694832/149‑1 (MQ=255) cACCAAGTTCTGAAGTCGGTCGCTTCTTTGAAACCCAGACATCAAACGGCGGGCGTTACACCGTCACGCTCCCGGATGGCACTAACGTCGAACAACTGAACAAAGCGACCGCAGCGATGATGGTCCCGTTCGACAGCATCAAATTctct < 1:276007/149‑1 (MQ=255) ccAAGTTCTGAAGTCGGTCGCTTCTTTGAACCCCAGTAATCAAATGGCGGGCGTTACACCGTCACGCTCCGGGTTGGCACTAAAGTCGAATAACTGAACAAAGCGACCGCAGCGATGATGGTCCCGTTCGACAGCATCAAATTCTCTgg < 2:3604333/149‑1 (MQ=255) cAAGTTCTGAAGTCGGTCGCTTCTTTGAAACCCAGTCATCAAAAGGCGGGCGTTACACCGTCACGCTCCCGGATGGCACTAAAGTCGAAGAACTGAACAAAGCGACCGCAGCGATGATGGTCCCGTTCGACAGCATCAAATTCTCTGGc > 2:1085858/1‑149 (MQ=255) aaGTTCTGAAGTCGGTCGCTTCTTTTAAAACCACTCATAAAAAGGCGGGCGTTGAACCGTCACCCACCCCGATTGCACTAAACTCGAAGAACAGAACAAAAAGACCCCAGCCATTTTGTTGCCGGTCGACACCAACAAAATCTCAGGCa > 1:3564061/1‑149 (MQ=255) gTTCTGAAGTGGGTCGCTTCTTTGAACCCCAGACAACAAAAGGCGGGCTTTACACCGTCACGCTCCCGGATGGCACTAAAGTCCAACAACTGAACAAAGCGACCGCAGCGATGATGGTCCCGTTCGACAGCATCAAATTCTCTGGCAAc < 1:4625334/149‑1 (MQ=255) cTGAAGTCGGTCTCTTATTTGAAACCCAGTCATAAAACGGCGGGCGTTACACCGTCACGCTCCCGGTTGGCACAAAAGTCGAAGAACTTAACAAAGCGACCGCAGCGATGATGGTCCCGTTCGACAGCATCAAATTCTCTGGCAACTAc < 1:1663719/149‑1 (MQ=255) gAAGTCGGTCGCTTCTTTGAAACCCAGTCATCAAAAGGCGGGCGTTACACCGTCACGCTCCCGGATGGCACTAAAGTCGAAGAACTGAACAAAGCGACCGCAGCGATGATGGTCCCGTTCGACAGCATCAAATTATATGGCAACAACgg > 2:5039577/1‑149 (MQ=255) gggtcgATTCTTTGAAACCCAGTCAACAAAAGGGGGGCGTTACACCGTCACGCTCCCGGATGGCACTAACGGCGAAGAACTGAACAACGCGACCGCAGCGATGATGGTCCCGTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACa < 2:1983094/148‑1 (MQ=255) cggtcgCTAATTTCAAACCCAGCCATCAAACGGCGGGCGTCACACCGTCCCGCTCCCGGCTGCCACTAACGACCAACAACTCACCAAAGCGACCGCAGCGATGATGGTCCCGTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACa < 1:2830521/149‑1 (MQ=255) gtgttcttTTAAACCCAGTCAAAAAACGGGGGGTGTTACACCGTCACGCGCCGGTTTGCCACTAAAGTCCACGAACTTAACAACGCGACCGCAGCGATGATGGTCCCGTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGAc < 1:46668/146‑1 (MQ=255) ggttttttttAAACCCTTTAATCAAAGGGGGGGGTTTACACGGTCACGCGCCGGGTTGGAAATAACGTCTAAGAACTTAAAAAAGCGACCGCAGCGATGATGGTCCCGTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGAc < 1:2675608/143‑1 (MQ=255) tcttTGAAACCCAGTCATCAAAAGGCGGGCGTTACACCGTCACGCTCCCGGATGGCACTAAAGTCGAAGAACTGAACAAAGCGACCGCAGCGATGATGGTCCCGTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGaa > 1:2540685/1‑149 (MQ=255) ttGAAACCCAGTCATCAAAAGGCGGGCTTTACACCGTCACGCTCCCGGATGGCACTAAAGTCGAAGAACTGAACAAAGCGACCGCAGCGATGATGGTCCCGTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGtc < 2:4455015/149‑1 (MQ=255) tGAAACCCAGTCATCAAAAGGCGGGCGTTACACCGTCACGCTCCCGGATGGCACTAAAGTCGAAGAACTGAACAAAGCGACCGCAGCGATGATGGTCCCGTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGtct > 1:1306799/1‑149 (MQ=255) aCCCAGTCATAAAAAGGGGGGCGTAACACCGTCACGCTCCCGGAGGGCACTAAAGTCCAATAACTGAAAAACGCGACCGCAGCGATGATGGTCCCGTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTa < 1:3246139/149‑1 (MQ=255) ccAGTCATCAAAAGGCGGGCGTTACACCGTCACGCTCCCGGATGGCACTAAAGTCGAATAACTGAACAAAGCGACCGCAGCGATGATGGTCCCGTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTATc < 2:577548/149‑1 (MQ=255) ccAGTCATCAAAAGGCGGGCGTTACACCGTCACGCTCCCGGATGGCACTAAAGTCGAAGAACTGAACAAAGCGACCGCAGCGATGATGGTCCCGTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTATc > 1:5132772/1‑149 (MQ=255) ccAGTCATCAAAAGGCGGGCGTTACACCGTCACGCTCCCGGATGGCACTAAAGTCGAAGAACTGAACAAAGCGACCGCAGCGATGATGGTCCCGTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTATc > 1:1831361/1‑149 (MQ=255) ccAGTCATAAAATGGGGGGGGTTACACGGTCACGCGCCGGGATGCCACTAATGTCTAAGAACTTAACAACGCGACCGCAGCGATGATGGTCCCGTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTATc < 1:695040/149‑1 (MQ=255) aGTCATCAAAAGGCGGGCGTTACACCGTCACGCTCCCGGATGGCACTAAAGTCGAAGAACTGAACAAAGCGACCGCAGCGATGATGGTCCCGTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTATCAg > 1:1064526/1‑149 (MQ=255) aGTCATCAAAAGGCGGGCGATACACCGTCACGCTCCCGGATGGCACTAAAGTCGAAGAACTGAACAAAGCGACCGCAGCGATGATGGTCCCGTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTATCAg > 2:1788821/1‑149 (MQ=255) gTCATCAAAAGGCGGGCGTTACACCGTCACGCTCCCGGATGGCACTAAAGTCGAAGAACTGAACAAAGCGACCGCAGCGATGATGGTCCCGTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTATCAgg > 2:215939/1‑149 (MQ=255) gTCATCAAAAGGCGGGCGTTACACCGTCACGCTCCCGGATGGCACTAAAGTCGAAGAACTGAACAAAGCGACCGCAGCGATGATGGTCCCGTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTATCAgg > 2:2182488/1‑149 (MQ=255) tcatcaAAAGGCGGGCGTTACACCGTCACGCTCCCGGATGGCACTAAAGTCGAAGAACTGAACAAAGCGACCGCAGCGATGATGGTCCCGTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTATCAGGt > 1:4535282/1‑149 (MQ=255) tcatcaAAAGGCGGGCGTTACACCGTCACGCTCCCGGATGGCACTAAAGTCGAAGAACTGAACAAAGCGACCGCAGCGATGATGGTCCCGTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTATCAGGt > 2:3983510/1‑149 (MQ=255) tcatcaAAAGGCGGGCGTTACACCGTCACGCTCCCGGATGGCACTAAAGTCGAAGAACTGAACAAAGCGACCGCAGCGATGATGGTCCCGTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTATCAGGt < 2:2894074/149‑1 (MQ=255) catcaAAAGGCGGGCGTTACACCGTCACGCTCCCGGATGGCACTAAAGTCGAAGAACTGAACAAAGCGACCGCAGCGATGATGGTCCCGTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTATCAGGtt < 2:3019309/149‑1 (MQ=255) tcaAAAGGCGGGCGTTACACCGTCACGCTCCCGGATGGCACTAAAGTCGAAGAACTGAACAAAGCGACCGCAGCGATGATGGTCCCGTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTATCAGGTTGc > 1:3422151/1‑149 (MQ=255) tcaAAAGGCGGGCGTTACACCGTCACGCTCCCGGATGGCACTAAAGTCGAAGAACTGAACAAAGCGACCGCAGCGATGATGGTCCCGTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTATCAGGTTGc < 2:4239399/149‑1 (MQ=255) | GAGCCCGGATGTGATCCCGGCAGATTCCGAAGCAGGACGTGCAGCTCTGGCTGCCGGTGGCGAAGCCGCGAAGAAAGTTGAGATCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTGAAACCCAGTCATCAAAAGGCGGGCGTTACACCGTCACGCTCCCGGATGGCACTAAAGTCGAAGAACTGAACAAAGCGACCGCAGCGATGATGGTCCCGTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTATCAGGTTGC > CP009273/1673232‑1673528 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |