Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,701,282 A→C 6.2% Q36P (CAG→CCG)  rsxC → SoxR iron‑sulfur cluster reduction factor component; putative membrane‑associated NADH oxidoreductase of electron transport complex

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,701,2820AC 6.2% 760.3 / 5.1 297Q36P (CAG→CCG) rsxCSoxR iron‑sulfur cluster reduction factor component; putative membrane‑associated NADH oxidoreductase of electron transport complex
Reads supporting (aligned to +/- strand):  ref base A (145/124);  new base C (25/2);  total (170/127)
Fisher's exact test for biased strand distribution p-value = 5.32e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 300 total aligned reads displayed.

ATCTGAACACCATTCCCGTGCGTATCATTCCCGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGCCAGCCGCTTACCCGTGGTCGCGGCAAAATGCTGCCTGTTCACGCGCCCACC  >  CP009273/1701134‑1701430
                                                                                                                                                    |                                                                                                                                                    
   tGAACACCATTCCCGTGCGTATCATTCCCGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGt                                                                                                                                                   >  2:5228046/1‑149 (MQ=255)
    gAACACCATTCCCGTGCGTATCATTCCCGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTa                                                                                                                                                  >  2:1713433/1‑149 (MQ=255)
    gAACACCATTCCCGTGCGTATCATTCCCGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCAGCGCCCGGTa                                                                                                                                                  >  1:4791358/1‑149 (MQ=255)
       cacCATTCCCGTGCGTATCATTCCCGTGGAACACCATGCTGAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCCTCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTcccc                                                                                                                                               >  2:4079251/1‑149 (MQ=255)
        acCATTCCCGTGCGTATCATTCCCGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTAcccc                                                                                                                                              <  2:4635297/149‑1 (MQ=255)
        acCATTCCCGTGCGTATCATTCCCGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTAcccc                                                                                                                                              <  1:2392050/149‑1 (MQ=255)
         ccATTCCCGTGCGTATCATTCCCGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCt                                                                                                                                             <  2:1373648/149‑1 (MQ=255)
         ccATTCCCGTGCGTATCATTCCCGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCt                                                                                                                                             >  2:2125344/1‑149 (MQ=255)
         ccATTCCCGTGCGTATCATTCCCGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCt                                                                                                                                             <  2:271239/149‑1 (MQ=255)
           aTTCCCGTGCGTATCATTCCCGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCCGGTACCCCTgg                                                                                                                                           >  2:883347/1‑149 (MQ=255)
            ttCCCGTGCGTATCATTCCCGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCCGGTACCCCTGgc                                                                                                                                          >  1:3252131/1‑149 (MQ=255)
                 gTGCGTATCATTCCCGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGc                                                                                                                                     >  2:2200718/1‑149 (MQ=255)
                      tATCATTCCCGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCCGCGgttt                                                                                                                                >  1:4671302/1‑149 (MQ=255)
                           ttCCCGTGTAACACCATGCTTAAGTTATTCTCTGCATTCATAAAAAATAAAATCTGGGATTTCAACGGCGGCATCAATCCACCGGAGATTAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTAt                                                                                                                           <  1:1563864/149‑1 (MQ=255)
                           tgcccGTGTAACACCTTGCTTAAGTTATTCTCTGAATACATAAAAAATAAATTCTGGTATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCGGGCGCAGCGTTTTGTTAt                                                                                                                           <  1:4071170/147‑1 (MQ=255)
                                  ggAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTg                                                                                                                    >  1:2986956/1‑149 (MQ=255)
                                     acacCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCCGGTACCCCAGGCGCCGCGTTTTGTTATTCCACTGaaa                                                                                                                 >  1:2103074/1‑149 (MQ=255)
                                     acacCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTACACTGaaa                                                                                                                 >  1:4253853/1‑149 (MQ=255)
                                       acCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCACCGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACa                                                                                                               <  2:4346261/149‑1 (MQ=255)
                                       acCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACa                                                                                                               >  2:120797/1‑149 (MQ=255)
                                       acCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTACACTGAAACa                                                                                                               >  2:1734684/1‑149 (MQ=255)
                                        ccATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAg                                                                                                              >  2:2707790/1‑149 (MQ=255)
                                        ccATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAg                                                                                                              >  2:572899/1‑149 (MQ=255)
                                          ctGCTTAAGTTATTCTCTGCATTCCGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCa                                                                                                            <  2:3821169/148‑1 (MQ=255)
                                          aTGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCa                                                                                                            <  2:1647685/149‑1 (MQ=255)
                                          aTGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCa                                                                                                            >  2:1483524/1‑149 (MQ=255)
                                             cTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCatat                                                                                                         <  2:2020967/149‑1 (MQ=255)
                                             cTTAAGTTAGTCTCGGCATTCAGAAAAAATAAAATCTGGGATTTCACCGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCGGCGCCAGGTACCCCTGGCGCAGCGTATTGTTATTCCACTGAAACAGCatat                                                                                                         <  2:4448070/149‑1 (MQ=255)
                                              ttAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCACCGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATAtt                                                                                                        <  1:2793804/149‑1 (MQ=255)
                                              ttAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATAtt                                                                                                        <  2:708462/149‑1 (MQ=255)
                                              ttAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGGTTTGTTATTCCACTGAAACAGCCTAtt                                                                                                        >  1:2742056/1‑149 (MQ=255)
                                              ttAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCGGGCGCAGCGGTTTGTTATTACACAGTAACAGCATAtt                                                                                                        >  1:578518/1‑149 (MQ=255)
                                              ttAAGTTATTCTCTGAATTCATAAAAAATAAAATCGGGTATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATAtt                                                                                                        <  1:615984/149‑1 (MQ=255)
                                                       tctcTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCCGGTACCCCTGGCGCAGCGTGTTGTTATTCCACTGAAACAGCATATTGGCGCTGaa                                                                                               >  2:2675966/1‑149 (MQ=255)
                                                       tctcTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGaa                                                                                               >  2:47668/1‑149 (MQ=255)
                                                         tctGCAGTCAGAAAAAATAAAATCGGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAgg                                                                                             <  2:4959553/149‑1 (MQ=255)
                                                          ctGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGt                                                                                            <  2:4142705/149‑1 (MQ=255)
                                                           tgatttCATAAAAAATAAAATGTGGGATTTCAACGGCGGCATCCATCCACCGTAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTg                                                                                           <  1:2313536/145‑1 (MQ=255)
                                                            gCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGa                                                                                          <  1:3898162/149‑1 (MQ=255)
                                                             cATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAg                                                                                         <  2:2828932/149‑1 (MQ=255)
                                                             cATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAg                                                                                         <  2:3138802/149‑1 (MQ=255)
                                                              aTTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCCGCGTTTTTTTTTTAAACAGAAACATCAAATTTTCGCTTAAGGTGAGt                                                                                        >  1:2244559/1‑149 (MQ=255)
                                                                tCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTtg                                                                                      <  1:365203/149‑1 (MQ=255)
                                                                  aGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCAGGCGCCGCGGGTTGTTATTACACTGAAACAGCCTATTGGCGCTGAAGGTGAGTtgtg                                                                                    >  1:2834272/1‑149 (MQ=255)
                                                                   gAAAAAATAAACTCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGc                                                                                   <  2:4828333/149‑1 (MQ=255)
                                                                   gAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGc                                                                                   <  1:3716645/149‑1 (MQ=255)
                                                                    aaaaaaTAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCg                                                                                  <  2:1949946/149‑1 (MQ=255)
                                                                     aaaaaTAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGt                                                                                 <  1:1970352/149‑1 (MQ=255)
                                                                      aaaataaaatCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGtt                                                                                <  1:4267146/149‑1 (MQ=255)
                                                                      aaaataaaatCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGtt                                                                                >  1:1725812/1‑149 (MQ=255)
                                                                         ataaaatCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCCGGTACCCCAGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGc                                                                             >  1:5034741/1‑149 (MQ=255)
                                                                          taaaatCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGGTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCg                                                                            >  1:4332525/1‑149 (MQ=255)
                                                                           aaaatCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCCGGTACCCCAGGCGCCGCGGTTTGTTATTACACGGAAAAACCAAAATTGCGCTGAAGGTGAGTTGTGGGTTAGCGt                                                                           >  1:4857027/1‑149 (MQ=255)
                                                                            aaatCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGGTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTc                                                                          >  2:4071342/1‑149 (MQ=255)
                                                                              atCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCgg                                                                        >  2:4690626/1‑149 (MQ=255)
                                                                               tCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGc                                                                       >  2:3007397/1‑149 (MQ=255)
                                                                               tCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGGGAGTTGTGCGTTAGCGTCGGc                                                                       >  2:2256585/1‑149 (MQ=255)
                                                                                 tGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCCGGTACCCCAGGCGCAGCGTTTTGTTATTGCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCgc                                                                     >  1:5008787/1‑148 (MQ=255)
                                                                                 tGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGa                                                                     <  1:741112/149‑1 (MQ=255)
                                                                                      tttCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAg                                                                >  2:3832006/1‑149 (MQ=255)
                                                                                      tttCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCGGGCGCAGCGGTTTGTTATTGCACTGAAACAGCATATTGGCGCTGAAGGTGGGTTGGGCGTTAGCGTCGGCGATAAAg                                                                >  1:180544/1‑149 (MQ=255)
                                                                                        tCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTa                                                              >  2:877759/1‑149 (MQ=255)
                                                                                         cAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCCGGTACCCCGGGCGCAGCGTTTTGTTATTCCACTGAAACAGCCGATTGGCGCGGAAGGTGAGGTGTGCGGTAGCGGCGGCGCTAAATTAt                                                             >  1:4990895/1‑149 (MQ=255)
                                                                                          aaCGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTAtt                                                            >  2:2180003/1‑149 (MQ=255)
                                                                                           aCGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTg                                                           >  2:5161496/1‑149 (MQ=255)
                                                                                           aCGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGGACCCCGGGCGCAGCGTGTTGTTATTCCACGGAAACAGCAGATTGGCGCGGAAGGTGAGGTGTGCGTTAGCGTCGGCGCTCAAGTATTg                                                           >  2:637133/1‑149 (MQ=255)
                                                                                            cggcggCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTgc                                                          <  2:1339781/149‑1 (MQ=255)
                                                                                            cggcggCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTgc                                                          <  2:3957741/149‑1 (MQ=255)
                                                                                              gcggcATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCCGGCGCCGCGTTTTGTTATTACAAGGAAAAAGCCTATTGGCGCAGAAGGTGTGTTTTGCGTTAGCGGCGGCGAGAAATTATTtcgc                                                        >  1:4504896/1‑149 (MQ=255)
                                                                                                 gcATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCCGGTACCCCTGGCGGAGCGTTTTGTTATTCCCCAGAAACAGCATATTGGCGCAGAAGGTGTGTTGTGGGTTAGCCGCGGCGATAAAGTAATGCGCGGc                                                     >  1:1111563/1‑149 (MQ=255)
                                                                                                    tccatccaCCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGCCAg                                                  >  2:4521242/1‑149 (MQ=255)
                                                                                                    tccatccaCCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGCCAg                                                  >  2:3744878/1‑149 (MQ=255)
                                                                                                    tccatccaCCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCAGGCGCAGCGTTTTGTTATTACACAGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTTCGCGGCCAg                                                  >  1:2490038/1‑149 (MQ=255)
                                                                                                      catccaCCGGAGATGAACACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGCCAGcc                                                <  2:3623013/149‑1 (MQ=255)
                                                                                                      catccaCCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTAACCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCCGAGTGGCGCTGCAGGTGAGTTGTGCGGTAGCGGCGGCGATAAAGTATTGCGCGGCCAGcc                                                >  1:3784900/1‑149 (MQ=255)
                                                                                                        tccaCCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCCGGTTCCCCGGGCGCAGCGTTTTGTTATTGCACGGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGGGTTTGCGGCGGCGCTAAAGTATTTCGCGGCCACCCGc                                              >  1:1468820/1‑149 (MQ=255)
                                                                                                         ccaCCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCGGGCGCCCCGGTTTGTTATTACACAGAAACACCATATTGGCGCTGTAGGTGTGTTGTGGGTTTTGGTCGGCGATAAAGTATTTCGCGGGCAGccccc                                             >  1:961717/1‑146 (MQ=255)
                                                                                                          caCCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGCCAGCCGCtt                                            <  2:2350480/149‑1 (MQ=255)
                                                                                                          caCCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGGTAGCGTCGGCGATAAAGTATTGCGCGGCCAGCCGCtt                                            >  2:4246917/1‑149 (MQ=255)
                                                                                                          caCCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGGTTTGTTATTCCACAGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGCTAAAGTATTGCGCGGCCAGCCGcgt                                            >  2:2095345/1‑147 (MQ=255)
                                                                                                                agaTGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGCCAGCCGCTTACCCGt                                      >  2:2434424/1‑149 (MQ=255)
                                                                                                                 gaTGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGCCAGCCGCTTACCCGTg                                     >  2:3501364/1‑149 (MQ=255)
                                                                                                                 gaTGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGCCAGCCGCTTACCCGTg                                     >  2:2974955/1‑149 (MQ=255)
                                                                                                                   tGAACACCCAGTCCAACGGTACCCCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGCCAGCCGCTTACCCGTGGt                                   <  1:3283828/149‑1 (MQ=255)
                                                                                                                   tGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGCCAGCCGCTTACCCGTGGt                                   <  2:511860/149‑1 (MQ=255)
                                                                                                                   tGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGCCAGCCGCTTACCCGTGGt                                   <  1:515761/149‑1 (MQ=255)
                                                                                                                       aaCCCAGTCCAACGGTACACCCCTGCGCCCGGTACCCCAGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTTGCGCTGAAGGTGAGTTTTGCGTTAGCGTCGGCGATAAAGTATTTCGCGGCCAGCCGCTTACCCCGGGTcgcg                               >  1:4167252/1‑149 (MQ=255)
                                                                                                                       aaCCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCAGATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGAGCGGCCAGCCGCTTACCCGTGGTcgcg                               >  2:1563782/1‑149 (MQ=255)
                                                                                                                             gTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGCCAGc                                                 <  1:700515/125‑1 (MQ=255)
                                                                                                                             gTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGCCAGc                                                 >  2:700515/1‑125 (MQ=255)
                                                                                                                             gTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGCCAGCCGCTTACCCGTGGTCGCGGCaaaa                         <  2:2097686/149‑1 (MQ=255)
                                                                                                                             gTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGCCAGCCGCTTACCCGTGGTCGCGGCaaaa                         <  1:2712052/149‑1 (MQ=255)
                                                                                                                                cAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGCCAGCCGCTTACCCGTGGTCGCGGCAAAAtgc                      <  1:2605135/149‑1 (MQ=255)
                                                                                                                                       acacCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGCCAGCCGCTTACCCGTGGTCGCGGCAAAATGCTGCCTGt               <  1:34547/149‑1 (MQ=255)
                                                                                                                                         acCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGCCAGCCGCTTACCCGTGGTCGCGGCACAATGCTGCCTGTTc             >  2:3380749/1‑149 (MQ=255)
                                                                                                                                          ccccTGCGCCAGGGACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGCCAGCCGCTTACCCGTGGTCGCGGCAAAATGCTGCCTGTTCa            <  1:3071728/149‑1 (MQ=255)
                                                                                                                                            catGCGCCTTGTACCACTGGCGCAGCGTTTTGTTATTCCACTGAATCAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATATAGTATTGCGCGGCCAGCCGCTTACCCGTGGTCGCGGCAAAATGCTGCCTGTTCAcg          <  1:4001401/147‑1 (MQ=255)
                                                                                                                                             cTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGCCAGCCGCTTACCCGTGGTCGCGGCAAAATGCTGCCTGTTCAcgc         <  2:587184/149‑1 (MQ=255)
                                                                                                                                             cTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGCCAGCCGCTTACCCGTGGTCGCGGCAAAATGCTGCCTGTTCAcgc         >  1:1280660/1‑149 (MQ=255)
                                                                                                                                                    aGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGCCAGCCGCTTACCCGTGGTCGCGGCAAAATGCTGCCTGTTCACGCGCCCAcc  <  1:553116/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
ATCTGAACACCATTCCCGTGCGTATCATTCCCGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGCCAGCCGCTTACCCGTGGTCGCGGCAAAATGCTGCCTGTTCACGCGCCCACC  >  CP009273/1701134‑1701430

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑