BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	1,784,952	A→G	53.7%	F184L (TTT→CTT)	ydiU ←	UPF0061 family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	1,784,952	0	A	G	53.7%	110.9 / inf	311	F184L (TTT→CTT)	ydiU	UPF0061 family protein
Reads supporting (aligned to +/- strand): ref base A (67/77); new base G (95/72); total (162/149)
Fisher's exact test for biased strand distribution p-value = 7.01e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 314 total aligned reads displayed.

TTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATCATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAAATGTTCGAAATGACCAAAGCGCAGATGACTTGGTGCCACACGCATCAGCATCGCGCCTGGCTCCGCCGTTTCCCGATACACTGGCGAATCGCTGGTGACGATACTTAACGCGCGGGTCGTCGGAATGCCCAGATAATGCATCGCCTCA  >  CP009273/1784806‑1785100

ttGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATCATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAAAt                                                                                                                                                    <  2:1397839/149‑1 (MQ=255)

 tGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATCATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAAATg                                                                                                                                                   <  1:4267378/149‑1 (MQ=255)

  gggCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATCATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTACAAATGt                                                                                                                                                  >  2:1705087/1‑149 (MQ=255)

  gggCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATCATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAAATGt                                                                                                                                                  <  2:1020510/149‑1 (MQ=255)

   ggCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATCATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAGATGtt                                                                                                                                                 <  1:2002839/149‑1 (MQ=255)

     cAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATCATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAAATGTTCg                                                                                                                                               <  2:2199890/149‑1 (MQ=255)

       aTTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATCATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAAATGTTCGaa                                                                                                                                             >  2:3259706/1‑149 (MQ=255)

         tAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATCATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAGATGTTCGAAAt                                                                                                                                           <  1:216396/149‑1 (MQ=255)

          aaCGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATCATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAGATGTTCGAAATg                                                                                                                                          >  2:1372139/1‑149 (MQ=255)

           acgacgCGGTACGTGCGACTACATCGCTAAACCAGAGACGGTATTTGTCCTCATCATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAAATGTTCGAAATGa                                                                                                                                         <  2:3394497/149‑1 (MQ=255)

           acgacgCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATCATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAAATGTTCGAAATGa                                                                                                                                         <  2:1921450/149‑1 (MQ=255)

            cgacgCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATCATCTGCAAGCTGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAGATGTTCGAAATGAc                                                                                                                                        <  2:396141/149‑1 (MQ=255)

                gcgGTACGTGCGACAACATCGCTAAACCAGAGACGGTAATTGTCCTCATCATCTACAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAAATGTTCGAAATGACCaaa                                                                                                                                    <  1:762026/149‑1 (MQ=255)

                  ggTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATCATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAAATGTTCGAAATGACCAAAgc                                                                                                                                  <  1:19266/149‑1 (MQ=255)

                       gTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATCATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAAATGTTCGAAATGACCAAAGCGCAGa                                                                                                                             <  2:822969/149‑1 (MQ=255)

                           gACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATCATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAAATGTTCGAAATGACCAAAGCGCAGATGAc                                                                                                                         >  1:1626359/1‑149 (MQ=255)

                             caacaTCGCTAAACCAGAGACGGTATTTGTCCTCATCATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAGATGTTCGAAATGACCAAAGCGCAGATGACtt                                                                                                                       >  2:4639931/1‑149 (MQ=255)

                             caacaTCGCTAAACCAGAGACGGTATTTGTCCTCATCATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAAATGTTCGAAATGACCAAAGCGCAGATGACtt                                                                                                                       <  1:2245183/149‑1 (MQ=255)

                              aacaTCGCTAAACCAGAGACGGTATTTGTCCTCATCATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAGATGTTCGAAATGACCAAAGCGCAGATGACTTg                                                                                                                      >  1:2316218/1‑149 (MQ=255)

                               acaTCGCTAAACCAGAGACGGTATTTGTCCTCATCATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAGATGTTCGAAATGACCAAAGCGCAGATGACTTgg                                                                                                                     >  1:4000377/1‑149 (MQ=255)

                               acaTCGCTAAACCAGAGACGGTATTTGTCCTCATCATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAGATGTTCGAAATGACCAAAGCGCAGATGACTTgg                                                                                                                     >  2:2710519/1‑149 (MQ=255)

                               acaTCGCTAAACCAGAGACGGTATTTGTCCTCATCATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAAATGTTCGAAATGACCAAAGCGCAGATGACTTgg                                                                                                                     >  1:1450348/1‑149 (MQ=255)

                               acaTCGCTAAACCAGAGACGGTATTTGTCCTCATCATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAAATGTTCGAAATGACCAAAGCGCAGATGACTTgg                                                                                                                     >  1:1118137/1‑149 (MQ=255)

                                caTCGCTAAACCAGAGACGGTATTTGTCCTCATCATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAAATGTTCGAAATGACCAAAGCGCAGATGACTTGGt                                                                                                                    >  1:3793383/1‑149 (MQ=255)

                                  tCGCTAAACCAGAGACGGTATTTGTCCTCATCATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAGATGTTCGAAATGACCAAAGCGCAGATGACTTGGTGc                                                                                                                  <  1:5104459/149‑1 (MQ=255)

                                  tCGCTAAACCAGAGACGGTATTTGTCCTCATCATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAAATGTTCGAAATGACCAAAGCGCAGATGACTTGGTGc                                                                                                                  <  1:3559673/149‑1 (MQ=255)

                                     cTAAACCAGAGACGGTATTTGTCCTCATCATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAGATGTTCGAAATGACCAAAGCGCAGATGACTTGGTGCcac                                                                                                               <  2:4765668/149‑1 (MQ=255)

                                       aaaCCAGAGACGGTATTTGTCCTCATCATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAAATGTTCGAAATGACCAAAGCGCAGATGACTTGGTGCCacac                                                                                                             >  1:2962431/1‑149 (MQ=255)

                                       aaaCCAGAGACGGTATTTGTCCTCATCATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAAATGTTCGAAATGACCAAAGCGCAGATGACTTGGTGCCacac                                                                                                             <  1:4059561/149‑1 (MQ=255)

                                         aCCAGAGACGGTATTTGTCCTCATCATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAGATGTTCGAAATGACCAAAGCGCAGATGACTTGGTGCCACACGc                                                                                                           >  1:1185634/1‑149 (MQ=255)

                                           cAGAGACGGTATTTGTCCTCATCATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAGATGTTCGAAATGACCAAAGCGCAGATGACTTGGTGCCACACGCAt                                                                                                         >  2:855633/1‑149 (MQ=255)

                                           cAGAGACGGTATTTGTCCTCATCATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAAATGTTCGAAATGACCAAAGCGCAGATGACTTGGTGCCACACGCAt                                                                                                         >  1:278907/1‑149 (MQ=255)

                                                    tATTTGTCCTCATCATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTACAGATGTTCGAAATGACCAAAGCGCAGATGACTTGGTGCCACACGCATCAGCATcgc                                                                                                >  2:4267165/1‑149 (MQ=255)

                                                    tATTTGTCCTCATCATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAGATGTTCGAAATGACCAAAGCGCAGATGACTTGGTGCCACACGCATCAGCATcgc                                                                                                <  1:3315519/149‑1 (MQ=255)

                                                    tATTTGTCCTCATCATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAGATGTTCGAAATGACCAAAGCGCAGATGACTTGGTGCCACACGCATCAGCATcgc                                                                                                >  2:649119/1‑149 (MQ=255)

                                                       ttGTCCTCATCATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAGATGTTCGAAATGACCAAAGCGCAGATGACTTGGTGCCACACGCATCAGCATCGCGcc                                                                                             <  1:358525/149‑1 (MQ=255)

                                                       ttGTCCTCATCATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAGATGTTCGAAATGACCAAAGCGCAGATGACTTGGTGCCACACGCATCAGCATCGCGcc                                                                                             >  1:374184/1‑149 (MQ=255)

                                                       ttGTCCTCATCATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAGATGTTCGAAATGACCAAAGCGCAGATGACTTGGTGCCACACGCATCAGCATCGCGcc                                                                                             >  1:1385144/1‑149 (MQ=255)

                                                           ccTCATCATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAGATGTTCGAAATGACCAAAGCGCAGATGACTTGGTGCCACACGCATCAGCATCGCGCCTGGc                                                                                         >  2:1230149/1‑149 (MQ=255)

                                                            cTCATCATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAGATGTTCGAAATGACCAAAGCGCAGATGACTTGGTGCCACACGCATCAGCATCGCGCCTGGCt                                                                                        <  1:4375970/149‑1 (MQ=255)

                                                             tcatcaTCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAGATGTTCGAAATGACCAAAGCGCAGATGACTTGGTGCCACACGCATCAGCATCGCGCCTGGCTc                                                                                       <  2:3548835/149‑1 (MQ=255)

                                                             tcatcaTCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAAATGTTCGAAATGACCAAAGCGCAGATGACTTGGTGCCACACGCATCAGCATCGCGCCTGGCTc                                                                                       <  2:410620/149‑1 (MQ=255)

                                                             tcatcaTCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAAATGTTCGAAATGACCAAAGCGCAGATGACTTGGTGCCACACGCATCAGCATCGCGCCTGGCTc                                                                                       <  1:1527251/149‑1 (MQ=255)

                                                              catcatCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAGATGTTCGAAATGACCAAAGCGCAGATGACTTGGTGCCACACGCATCAGCATCGCGCCTGGCTcc                                                                                      >  1:2902383/1‑149 (MQ=255)

                                                               atcatcTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAAATGTTCGAAATGACCAAAGCGCAGATGACTTGGTGCCACACGCATCAGCATCGCGCCTGGCTccg                                                                                     >  1:622577/1‑149 (MQ=255)

                                                                tcatcTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAAATGTTCGAAATGACCAAAGCGCAGTTGACTTGGTGCCACACGCATCAGCATCGCGCCTGGCTccgc                                                                                    <  2:3244970/149‑1 (MQ=255)

                                                                      gCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAAATGTTCGAAATGACCAAAGCGCAGATGACTTGGTGCCACACGCATCAGCATCGCGCCTGGCTCCGCCGTTTc                                                                              <  2:545033/149‑1 (MQ=255)

                                                                       cAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAAATGTTCGAAATGACCAAAGCGCAGATGACTTGGTGCCACACGCATCAGCATCGCGCCTGGCTCCGCCGTTTcc                                                                             >  2:3311363/1‑149 (MQ=255)

                                                                       cAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAAATGTTCGAAATGACCAAAGCGCAGATGACTTGGTGCCACACGCATCAGCATCGCGCCTGGCTCCGCCGTTTcc                                                                             <  1:321584/149‑1 (MQ=255)

                                                                          gATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAGATGTTCGAAATGACCAAAGCGCAGATGACTTGGTGCCACACGCATCAGCATCGCGCCTGGCTCCGCCGTTTCCCGa                                                                          >  2:4480333/1‑149 (MQ=255)

                                                                             gtgACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAGATGTTCGAAATGACCAAAGCGCAGATGACTTGGTGCCACACGCATCAGCATCGCGCCTGGCTCCGCCGTTTCCCGATac                                                                       >  2:1506932/1‑149 (MQ=255)

                                                                              tgACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAGATGTTCGAAATGACCAAAGCGCAGATGACTTGGTGCCACACGCATCAGCATCGCGCCTGGCTCCGCCGTTTCCCGATaca                                                                      <  2:3911955/149‑1 (MQ=255)

                                                                              tgACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAGATGTTCGAAATGACCAAAGCGCAGATGACTTGGTGCCACACGCATCAGCATCGCGCCTGGCTCCGCCGTTTCCCGATaca                                                                      <  1:2214236/149‑1 (MQ=255)

                                                                               gACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAGATGTTCGAAATGACCAAAGCGCAGATGACTTGGTGCCACACGCATCAGCATCGCGCCTGGCTCCGCCGTTTCCCGATacac                                                                     >  1:4571383/1‑149 (MQ=255)

                                                                                aCCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAGATGTTCGAAATGACCAAAGCGCAGATGACTTGGTGCCACACGCATCAGCATCGCGCCTGGCTCCGCCGTTTCCCGATACACt                                                                    >  2:4654843/1‑149 (MQ=255)

                                                                                 ccAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAAATGTTCGAAATGACCAAAGCGCAGATGACTTGGTGCCACACGCATCAGCATCGCGCCTGGCTCCGCCGTTTCCCGATACACTg                                                                   >  1:3837651/1‑149 (MQ=255)

                                                                                   aGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAAATGTTCGAAATGACCAAAGCGCAGATGACTTGGTGCCACACGCATCAGCATCGCGCCTGGCTCCGCCGTTTCCCGATACACTGGc                                                                 >  1:2492455/1‑149 (MQ=255)

                                                                                      aaTGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAGATGTTCGAAATGACCAAAGCGCAGATGACTTGGTGCCACACGCATCAGCATCGCGCCTGGCTCCGCCGTTTCCCGATACACTGGCGaa                                                              >  1:1744414/1‑149 (MQ=255)

                                                                                       aTGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAGATGTTCGAAATGACCAAAGCGCAGATGACTTGGTGCCACACGCATCAGCATCGCGCCTGGCTCCGCCGTTTCCCGATACACTGGCGAAt                                                             >  2:264600/1‑149 (MQ=255)

                                                                                       aTGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAGATGTTCGAAATGACCAAAGCGCAGATGACTTGGTGCCACACGCATCAGCATCGCGCCTGGCTCCGCCGTTTCCCGATACACTGGCGAAt                                                             <  1:2393062/149‑1 (MQ=255)

                                                                                       aTGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAGATGTTCGAAATGACCAAAGCGCAGATGACTTGGTGCCACACGCATCAGCATCGCGCCTGGCTCCGCCGTTTCCCGATACACTGGCGAAt                                                             >  2:2348216/1‑149 (MQ=255)

                                                                                       aTGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAAATGTTCGAAATGACCAAAGCGCAGATGACTTGGTGCCACACGCATCAGCATCGCGCCTGGCTCCGCCGTTTCCCGATACACTGGCGAAt                                                             <  1:1352508/149‑1 (MQ=255)

                                                                                       aTGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAAATGTTCGAAATGACCAAAGCGCAGATGACTTGGTGCCACACGCATCAGCATCGCGCCTGGCTCCGCCGTTTCCCGATACACTGGCGAAt                                                             <  1:3970460/149‑1 (MQ=255)

                                                                                          aCGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAAATGTTCGAAATGACCAAAGCGCAGATGACTTGGTGCCACACGCATCAGCATCGCGCCTGGCTCCGCCGTTTCCCGATACACTGGCGAATCGc                                                          >  2:617145/1‑149 (MQ=255)

                                                                                          aCGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAAATGTTCGAAATGACCAAAGCGCAGATGACTTGGTGCCACACGCATCAGCATCGCGCCTGGCTCCGCCGTTTCCCGATACACTGGCGAATCGc                                                          <  2:4698922/149‑1 (MQ=255)

                                                                                           cGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAGATGTTCGAAATGACCAAAGCGCAGATGACTTGGTGCCACACGCATCAGCATCGCGCCTGGCTCCGCCGTTTCCCGATACACTGGCGAATCGCt                                                         <  1:1565940/149‑1 (MQ=255)

                                                                                           cGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAGATGTTCGAAATGACCAAAGCGCAGATGACTTGGTGCCACACGCATCAGCATCGCGCCTGGCTCCGCCGTTTCCCGATACACTGGCGAATCGCt                                                         <  1:4710998/149‑1 (MQ=255)

                                                                                           cGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAAATGTTCGAAATGACCAAAGCGCAGATGACTTGGTGCCACACGCATCAGCATCGCGCCTGGCTCCGCCGTTTCCCGATACACTGGCGAATCGCt                                                         >  1:3608673/1‑149 (MQ=255)

                                                                                            gAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAAATGTTCGAAATGACCAAAGCGCAGATGACTTGGTGCCACACGCATCAGCATCGCGCCTGGCTCCGCCGTTTCCCGATACACTGGCGAATCGCTg                                                        >  2:2379383/1‑149 (MQ=255)

                                                                                             aaTAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAAATGTTCGAAATGACCAAAGCGCAGATGACTTGGTGCCACACGCATCAGCATCGCGCCTGGCTCCGCCGTTTCCCGATACACTGGCGACTCGCTgg                                                       >  2:4585551/1‑149 (MQ=255)

                                                                                               tAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAGATGTTCGAAATGACCAAAGCGCAGATGACTTGGTGCCACACGCATCAGCATCGCGCCTGGCTCCGCCGTTTCCCGATACACTGGCGAATCGCTGGTg                                                     >  2:2742797/1‑149 (MQ=255)

                                                                                                aGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAAATGTTCGAAATGACCAAAGCGCAGATGACTTGGTGCCACACGCATCAGCATCGCGCCTGGCTCCGCCGTTTCCCGATACACTGGCGAATCGCTGGTGa                                                    <  1:4526035/149‑1 (MQ=255)

                                                                                                   aaaGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAGATGTTCGAAATGACCAAAGCGCAGATGACTTGGTGCCACACGCATCAGCATCGCGCCTGGCTCCGCCGTTTCCCGATACACTGGCGAATCGCTGGTGACGa                                                 <  1:2580750/149‑1 (MQ=255)

                                                                                                   aaaGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAAATGTTCGAAATGACCAAAGCGCAGATGACTTGGTGCCACACGCATCAGCATCGCGCCTGGCTCCGCCGTTTCCCGATACACTGGCGAATCGCTGGTGACGa                                                 >  1:4971307/1‑149 (MQ=255)

                                                                                                     aGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAAATGTTCGAAATGACCAAAGCGCAGATGACTTGGTGCCACACGCATCAGCATCGCGCCTGGCTCCGCCGTTTCCCGATACACTGGCGAATCGCTGGTGACGATa                                               >  2:2502505/1‑149 (MQ=255)

                                                                                                         aGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAAATGTTCGAAATGACCAAAGCGCAGATGACTTGGTGCCACACGCATCAGCATCGCGCCTGGCTCCGCCGTTTCCCGATACACTGGCGAATCGCTGGTGACGATACTTa                                           >  1:501390/1‑149 (MQ=255)

                                                                                                          gCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAGATGTTCGAAATGACCAAAGCGCAGATGACTTGGTGCCACACGCATCAGCATCGCGCCTGGCTCCGCCGTTTCCCGATACACTGGCGAATCGCTGGTGACGATACTTaa                                          >  2:4026493/1‑149 (MQ=255)

                                                                                                          gCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAAATGTTCGAAATGACCAAAGCGCAGATGACTTGGTGCCACACGCATCAGCATCGCGCCTGGCTCCGCCGTTTCCCGATACACTGGCGAATCGCTGGTGACGATACTTaa                                          >  2:2898349/1‑149 (MQ=255)

                                                                                                           cTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAAATGTTCGAAATGACCAAAGCGCAGATGACTTGGTGCCACACGCATCAGCATCGCGCCTGGCTCCGCCGTTTCCCGATACACTGGCGAATCGCTGGTGACGATACTTAAc                                         <  2:2092388/149‑1 (MQ=255)

                                                                                                             aaCTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAAATGTTCGAAATGACCAAAGCGCAGATGACTTGGTGCCACACGCATCAGCATCGCGCCTGGCTCCGCCGTTTCCCGATACACTGGCGAATCGCTGGTGACGATACTTAAcgc                                       >  2:3044135/1‑149 (MQ=255)

                                                                                                              aCTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAGATGTTCGAAATGACCAAAGCGCAGATGACTTGGTGCCACACGCATCAGCATCGCGCCTGGCTCCGCCGTTTCCCGATACACTGGCGAATCGCTGGTGACGATACTTAAcgcg                                      >  2:2616576/1‑149 (MQ=255)

                                                                                                                tGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAAATGTTCGAAATGACCAAAGCGCAGATGACTTGGTGCCACACGCATCAGCATCGCGCCTGGCTCCGCCGTTTCCCGATACACTGGCGAATCGCTGGTGACGATACTTAAcgcgcg                                    >  2:2766157/1‑149 (MQ=255)

                                                                                                                 gACGAACCTTTTCCGACTCGCGGCGGTAGTAAAAATGTTCGAAATGACCAAAGCGCAGATGACTTGGTGCCACACGCATCAGCATCGCGCCTGGCTCCGCCGTTTCCCGATACACTGGCGAATCGCTGGTGACGATACTTAACGCGCgg                                   >  2:5036469/1‑149 (MQ=255)

                                                                                                                    gAACCTTTTCCGACTCGCGGCGGTAGTAAAGATGTTCGAAATGACCAAAGCGCAGATGACTTGGTGCCACACGCATCAGCATCGCGCCTGGCTCCGCCGTTTCCCGATACACTGGCGAATCGCTGGTGACGATACTTAACGCGCGGgtc                                >  1:1086303/1‑149 (MQ=255)

                                                                                                                        cTTTTCCGACTCGCGGCGGTAGTAAAAATGTTCGAAATGACCAAAGCGCAGATGACTTGGTGCCACACGCATCAGCATCGCGCCTGGCTCCGCCGTTTCCCGATACACTGGCGAATCGCTGGTGACGATACTTAACGCGCGGGtcgtcg                            >  2:509696/1‑149 (MQ=255)

                                                                                                                         ttttCCGACTCGCGGCGGTAGTAAAGATGTTCGAAATGACCAAAGCGCAGATGACTTGGTGCCACACGCATCAGCATCGCGCCTGGCTCCGCCGTTTCCCGATACACTGGCGAATCGCTGGTGACGATACTTAACGCGCGGGTCGTCgg                           >  1:1230181/1‑149 (MQ=255)

                                                                                                                          tttCCGACTCGCGGCGGTAGTAAAGATGTTCGAAATGACCAAAGCGCAGATGACTTGGTGCCACACGCATCAGCATCGCGCCTGGCTCCGCCGTTTCCCGATACACTGGCGAATCGCTGGTGACGATACTTAACGCGCGGGTCGTCGGa                          >  2:1663940/1‑149 (MQ=255)

                                                                                                                            tCCGACTCGCGGCGGTAGTAAAGATGTTCGAAATGACCAAAGCGCAGATGACTTGGTGCCACACGCATCAGCATCGCGCCTGGCTCCGCCGTTTCCCGATACACTGGCGAATCGCTGGTGACGATACTTAACGCGCGGGTCGTCGGAAt                        <  1:4840847/149‑1 (MQ=255)

                                                                                                                            tCCGACTCGCGGCGGTAGTAAAGATGTTCGAAATGACCAAAGCGCAGATGACTTGGTGCCACACGCATCAGCATCGCGCCTGGCTCCGCCGTTTCCCGATACACTGGCGAATCGCTGGTGACGATACTTAACGCGCGGGTCGTCGGAAt                        <  1:2222745/149‑1 (MQ=255)

                                                                                                                            tCCGACTCGCGGCGGTAGTAAAAATGTTCGAAATGACCAAAGCGCAGATGACTTGGTGCCACACGCATCAGCATCGCGCCTGGCTCCGCCGTTTCCCGATACACTGGCGAATCGCTGGTGACGATACTTAACGCGCGGGTCGTCGGAAt                        >  2:2287644/1‑149 (MQ=255)

                                                                                                                              cGACTCGCGGCGGTAGTAAAGATGTTCGAAATGACCAAAGCGCAGATGACTTGGTGCCACACGCATCAGCATCGCGCCTGGCTCCGCCGTTTCCCGATACACTGGCGAATCGCTGGTGACGATACTTAACGCGCGGGTCGTCGGAATGc                      <  2:4008760/149‑1 (MQ=255)

                                                                                                                              cGACTCGCGGCGGTAGTAAAGATGTTCGAAATGACCAAAGCGCAGATGACTTGGTGCCACACGCATCAGCATCGCGCCTGGCTCCGCCGTTTCCCGATACACTGGCGAATCGCTGGTGACGATACTTAACGCGCGGGTCGTCGGAATGc                      >  1:2620043/1‑149 (MQ=255)

                                                                                                                                  tCGCGGCGGTAGTAAAGATGTTCGAAATGACCAAAGCGCAGATGACTTGGTGCCACACGCATCAGCATCGCGCCTGGCTCCGCCGTTTCCCGATACACTGGCGAATCGCTGGTGACGATACTTAACGCGCGGGTCGTCGGAATGCCCAg                  >  2:2476148/1‑149 (MQ=255)

                                                                                                                                   cgcgGCGGTAGTAAAAATGTTCGAAATGACCAAAGCGCAGATGACTTGGTGCCACACGCATCAGCATCGCGCCTGGCTCCGCCGTTTCCCGATACACTGGCGAATCGCTGGTGACGATACTTAACGCGCGGGTCGTCGGAATGCCCAGa                 <  1:839631/149‑1 (MQ=255)

                                                                                                                                   cgcgGCGGTAGTAAAAATGTTCGAAATGACCAAAGCGCAGATGACTTGGTGCCACACGCATCAGCATCGCGCCTGGCTCCGCCGTTTCCCGATACACTGGCGAATCGCTGGTGACGATACTTAACGCGCGGGTCGTCGGAATGCCCAGa                 <  1:1690029/149‑1 (MQ=255)

                                                                                                                                      ggcggTAGTAAAGATGTTCGAAATGACCAAAGCGCAGATGACTTGGTGCCACACGCATCAGCATCGCGCCTGGCTCCGCCGTTTCCCGATACACTGGCGAATCGCTGGTGACGATACTTAACGCGCGGGTCGTCGGAATGCCCAGATaa              <  2:2029240/149‑1 (MQ=255)

                                                                                                                                      ggcggTAGTAAAGATGTTCGAAATGACCAAAGCGCAGATGACTTGGTGCCACACGCATCAGCATCGCGCCTGGCTCCGCCGTTTCCCGATACACTGGCGAATCGCTGGTGACGATACTTAACGCGCGGGTCGTCGGAATGCCCAGATaa              <  1:1872832/149‑1 (MQ=255)

                                                                                                                                            agtaAAGATGTTCGAAATGACCAAAGCGCAGATGACTTGGTGCCACACGCATCAGCATCGCGCCTGGCTCCGCCGTTTCCCGATACACTGGCGAATCGCTGGTGACGATACTTAACGCGCGGGTCGTCGGAATGCCCAGATAATGCATc        >  1:1002681/1‑149 (MQ=255)

                                                                                                                                                 aaaTGTTCGAAATGACCAAAGCGCAGATGACTTGGTGCCACACGCATCAGCATCGCGCCTGGCTCCGCCGTTTCCCGATACACTGGCGAATCGCTGGTGACGATACTTAACGCGCGGGTCGTCGGAATGCCCAGATAATGCATCGCCTc   >  2:908218/1‑149 (MQ=255)

                                                                                                                                                  aaTGTTCGAAATGACCAAAGCGCAGATGACTTGGTGCCACACGCATCAGCATCGCGCCTGGCTCCGCCGTTTCCCGATACACTGGCGAATCGCTGGTGACGATACTTAACGCGCGGGTCGTCGGAATGCCCAGATAATGCATCGCCTCa  >  2:4379730/1‑149 (MQ=255)

TTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATCATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAAATGTTCGAAATGACCAAAGCGCAGATGACTTGGTGCCACACGCATCAGCATCGCGCCTGGCTCCGCCGTTTCCCGATACACTGGCGAATCGCTGGTGACGATACTTAACGCGCGGGTCGTCGGAATGCCCAGATAATGCATCGCCTCA  >  CP009273/1784806‑1785100

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`