Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,823,835 A→C 6.2% W53G (TGG→GGG)  astD ← succinylglutamic semialdehyde dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,823,8350AC 6.2% 894.8 / 3.6 362W53G (TGG→GGG) astDsuccinylglutamic semialdehyde dehydrogenase
Reads supporting (aligned to +/- strand):  ref base A (213/111);  new base C (3/30);  total (216/146)
Fisher's exact test for biased strand distribution p-value = 1.63e-10
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 369 total aligned reads displayed.

ATCATCGCCGTCACTTCGGTTGCCGCTTCCCAGCGCGGCTTACCCGTTTCTCTGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATGCGCCCTGGCCCGTTATCCAGTCACCGTTAATCCAT  >  CP009273/1823687‑1823983
                                                                                                                                                    |                                                                                                                                                    
atcatcGCCGTCACTTCGGTTGCCGCTTCCCAGCGCGGCTTACCCGTTTCTCTGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCa                                                                                                                                                      >  2:4302470/1‑149 (MQ=255)
  catcGCCGTCACTTCGGTTGCCGCTTCCCAGCGCGGCTTACCCGTTTCTCTGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAgc                                                                                                                                                    <  1:305237/149‑1 (MQ=255)
       ccGTCACTTCGGTTGCCGCTTCCCAGCGCGGCTTACCCGTTTCTCTGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGa                                                                                                                                               >  2:1923885/1‑149 (MQ=255)
       ccGTCACTTCGGTTGCCGCTTCCCAGCGCGGCTTACCCGTTTCTCTGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGa                                                                                                                                               >  2:2126437/1‑149 (MQ=255)
       ccGTCACTTCGGTTGCCGCTTCCCAGCGCGGCTTACCCGTTTCTCTGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGa                                                                                                                                               >  1:5201532/1‑149 (MQ=255)
         gTCACTTCGGTTGCCGCTTCCCAGCGCGGCTTACCCGTTTCTCTGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGaaa                                                                                                                                             <  2:4740201/149‑1 (MQ=255)
         gTCACTTCGGTTGCCGCTTCCCAGCGCGGCTTACCCGTTTCTCTGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGaaa                                                                                                                                             >  1:5143257/1‑149 (MQ=255)
          tCACTTCGGTTGCCGCTTCCCAGCGCGGCTTACCCGTTTCTCTGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAGGCCGCGCCCCGCGCGGAAAc                                                                                                                                            >  1:4624320/1‑149 (MQ=255)
          tCACTTCGGTTGCCGCTTCCCAGCGCGGCTTACCCGTTTCTCTGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAAc                                                                                                                                            >  2:4177560/1‑149 (MQ=255)
             cTTCGGTTGCCGCTTCCCAGCGCGGCTTACCCGTTTCTCTGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAAcgcc                                                                                                                                         >  1:307848/1‑149 (MQ=255)
                 ggTTGCCGCTTCCCAGCGCGGCTTACCCGTTTCTCTGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCAc                                                                                                                                     >  2:72317/1‑149 (MQ=255)
                  gTTGCCGCTTCCCAGCGCGGCTTACCCGTTTCTCTGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACg                                                                                                                                    >  1:84416/1‑149 (MQ=255)
                   ttGCCGCTTCCCAGCGCGGCTTACCCGTTTCTCTGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACgg                                                                                                                                   >  1:823161/1‑149 (MQ=255)
                    tGCCGCTTCCCAGCGCGGCTTACCCGTTTCTCTGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACggg                                                                                                                                  >  1:2618249/1‑149 (MQ=255)
                     gCCGCTTCCCCGCGCGGCTTACCCGTTTCTCGGGAAATAATGGCGGTTAATGCGGATTTATTGATTTCCAGCTGTGCGGAAACGGGTTCGACACCGGCATGACGTTCAGAAAATGAAAGCCGCGCCCAGCGGGGAAACGCCGCACGGGc                                                                                                                                 <  2:493763/149‑1 (MQ=255)
                     gCCGCTCCCCCGCGCGGCTTACCCTTTTCTCTGGAAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGAAAATTAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGc                                                                                                                                 <  2:3210941/149‑1 (MQ=255)
                     gCCGATTCCCAGCGCGGATTACCCGTTTCTCTGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGc                                                                                                                                 <  2:1748179/149‑1 (MQ=255)
                       cGCTTCCCAGCGCGGCTTACCCGTTTCTCTGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTg                                                                                                                               >  1:652350/1‑149 (MQ=255)
                        gattCCCAGCGCGGCTTACCCGTTTCTCTGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGc                                                                                                                              >  1:1074863/3‑149 (MQ=255)
                         cTTCCCAGCGCGGTTTACCGTTTTCTGGGGAAATAATCGGGGTTATTTGGGCTTTTTTGCTTTCCCGCTGTGCGGAAACGCTTTCGACAACGGCTTGAGTTTCAGAAAATCAAAGCCGCGCCCAGCGCGTAAACGCCGCACGGGCTGcc                                                                                                                             <  1:4341616/149‑1 (MQ=255)
                         cTTCCCAGCGCGGCTTACCCGTTTCTCTGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGcc                                                                                                                             >  1:4913783/1‑149 (MQ=255)
                           tCCCAGCGCGGCTTACCCGTTTCTCTGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCg                                                                                                                           >  2:137330/1‑149 (MQ=255)
                           tCCCAGCGCGGCTTACCCGTTTCTCTGGCAATAATCGCGGTTAATTCGGCGTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCg                                                                                                                           >  1:2017742/1‑149 (MQ=255)
                               aGCGCGGCTTACCCGTTTCTCTGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACaa                                                                                                                       >  1:3495001/1‑149 (MQ=255)
                                   gggCTAACCCGTTTCTCTGGAAATATTCGGGGTTATTTGGGCTTTTTGGCTTTCCAGCCGTGCGGCAAAGCGTTCGACACCGGAATGACGTGCAGAAAATGAAAGCCGCGCCCCGCGCGGACACGCCGCACGGGCTGCCCGACAAGCCt                                                                                                                   <  1:3457419/148‑1 (MQ=255)
                                    ggCTTACCCGTTTCTCTGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCACATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTg                                                                                                                  >  2:1647061/1‑149 (MQ=255)
                                     gCTTACCCGTTTCTCTGGAAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCCGCTGTGCGGCAACGCGTTCGACAACGGCTTGACGTTCAGCAAATGAAAGCCGCGCCCCGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGc                                                                                                                 <  2:3537573/149‑1 (MQ=255)
                                       ttACCCGTTTCTCTGGCAATAATCGCGGTTAATTCGGCTTTTTGGCTTTCCAGCAGTGCGGAAACGCGTTCGACACCGGCATGACGTTCCGCAAATGAAAGCCGCGCCCCGCGCGGACACGCCGCACGGGCTGCCCGACAAGCCTGCTc                                                                                                               <  2:1013076/149‑1 (MQ=255)
                                         aCCCGTTTCTCTGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGa                                                                                                             <  2:3764201/149‑1 (MQ=255)
                                          cccGTTTCTCTGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGAc                                                                                                            >  1:5028965/1‑149 (MQ=255)
                                            cGTTTCTCTGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCt                                                                                                          >  1:3307954/1‑149 (MQ=255)
                                            cGTTTCTCGGGCAATAATCGCGGTAAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCt                                                                                                          <  2:3382936/149‑1 (MQ=255)
                                              tttCTCTGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGa                                                                                                        >  2:2425543/1‑149 (MQ=255)
                                                tctcTGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGc                                                                                                      >  2:2714737/1‑149 (MQ=255)
                                                 ctctGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGcg                                                                                                     >  2:3960593/1‑149 (MQ=255)
                                                  tgggcaaataatCGCGTTTATTTGGGATTTATTGCTTTCCCCCTGTGCGGCAAAGCGTTCGACACCGGCTTGACGTACAGCAAATGAACGCCGCGCCCAGCGCGCAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGcgg                                                                                                    <  1:2278175/143‑1 (MQ=255)
                                                   ctGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGcggc                                                                                                   <  2:262971/149‑1 (MQ=255)
                                                    tttaaataatCGCTTTTAATTCGGATTTATTGATTACCAGCTGTGCGGAAAAGCTTTCTAAAACGCAATGACTTACACAAAATCAAAGCCGCGCCAAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGcggca                                                                                                  <  1:5055000/145‑1 (MQ=255)
                                                    tGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGcggca                                                                                                  >  1:887524/1‑149 (MQ=255)
                                                    gggCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTAAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGcggca                                                                                                  <  2:5021017/148‑1 (MQ=255)
                                                      gCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGcggcatc                                                                                                <  2:1272114/149‑1 (MQ=255)
                                                      gCAATAAGCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAACGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGcggcatc                                                                                                <  2:3634599/149‑1 (MQ=255)
                                                       cAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGcggcatcg                                                                                               >  2:921224/1‑149 (MQ=255)
                                                           aatCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGcggcatcggcat                                                                                           >  2:1810904/1‑149 (MQ=255)
                                                            atcggttttAATTGGGCTTTTTTGCTTGCCCGCGGTGGGGCAAAGCGTTCGACAACGGCTTGACTTACACAAAATGAACGCCGCGCCCCGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCggcatcggcatc                                                                                          <  1:3717774/142‑1 (MQ=255)
                                                             gcggtgTTATTTCGGTTTTTTTGCTTTCCAGCTGGGCGGAAACGGGTTCGACACCGGCTTTCCGTTCAGAAAATCAAAGCCGCGCCCCGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGcatca                                                                                         <  1:197492/144‑1 (MQ=255)
                                                              cgggtttATTTCGGCTTTTTTGCTTTCCCGCTGTGCGGAAAAGGTTTCCACAACGGAATCACTTTAACCAAATTAACGCCGCCCCCAGCGGGCAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGcatcat                                                                                        <  1:4111652/144‑1 (MQ=255)
                                                               ggggTTAATTGGTTTTTTTTGTTTTCCTGCTGTGGGGAAATGCGTTCGAAATCGGATTTAGTTTCATAAATTGAAAGCCGCGCCCAGCGCGCAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCAtt                                                                                       <  1:4530086/147‑1 (MQ=255)
                                                               gcgGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCAtt                                                                                       >  1:4078942/1‑149 (MQ=255)
                                                                 ggTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCAttgc                                                                                     >  1:2418401/1‑149 (MQ=255)
                                                                   ttAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAATGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCAttgcct                                                                                   <  2:3338303/149‑1 (MQ=255)
                                                                      aTTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCAttgccttgc                                                                                >  1:1458466/1‑149 (MQ=255)
                                                                      aTTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCAttgccttgc                                                                                >  2:4515949/1‑149 (MQ=255)
                                                                      aTTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCAttgccttgc                                                                                >  2:4157788/1‑149 (MQ=255)
                                                                       ttCGGATTTATTGCTTTCCAGCTGTGCGGAAAAGCGTTCGAAAACGGCATGACTTACAGCAAATCAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCAttgccttgcc                                                                               <  1:323229/149‑1 (MQ=255)
                                                                        tCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGCCACCGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCa                                                                              <  2:2103467/149‑1 (MQ=255)
                                                                            cTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAac                                                                          >  1:1839904/1‑149 (MQ=255)
                                                                            cTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAac                                                                          <  2:3055462/149‑1 (MQ=255)
                                                                              ttATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAacac                                                                        <  2:2902555/149‑1 (MQ=255)
                                                                               ttttGCTTCCCCGCTGTGCGGCAAAGCGTTCGACACCGGCATGACGTCCAGCAAATGAAAGCCGCGCCCCGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACAcc                                                                       <  1:2126437/147‑1 (MQ=255)
                                                                                aTTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCt                                                                      >  1:4806449/1‑149 (MQ=255)
                                                                                aTTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCt                                                                      >  1:2856376/1‑149 (MQ=255)
                                                                                  tGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCg                                                                    >  2:315297/1‑149 (MQ=255)
                                                                                       tCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGa                                                               >  2:441582/1‑149 (MQ=255)
                                                                                         cagcagTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATa                                                             >  1:577720/1‑149 (MQ=255)
                                                                                          agcagTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATAc                                                            >  1:1636193/1‑149 (MQ=255)
                                                                                             agTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCgg                                                         >  2:171441/1‑149 (MQ=255)
                                                                                             agTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCgg                                                         >  2:5105051/1‑149 (MQ=255)
                                                                                              gTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCCGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGa                                                        >  2:1112076/1‑149 (MQ=255)
                                                                                               tGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGTAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGAt                                                       <  1:439751/149‑1 (MQ=255)
                                                                                                 cgtaaaaGCTTTCGACAACGGATTGACGTTCAGAAAATTAAAGCCGCGCCCAGCGCGCAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTa                                                     <  1:2379002/145‑1 (MQ=255)
                                                                                                 cGGCACAGCTTTCGACAACGGAATGACGTACAGAAACTGAAAGCCGCGCCCTGCGCGCAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTa                                                     <  1:3140152/149‑1 (MQ=255)
                                                                                                        gCGTTCGACACCGGAATTACTTACAGAAAATTAACGCCGCGCCCCGCGCGCAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTca                                              <  1:959070/149‑1 (MQ=255)
                                                                                                        gCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTca                                              >  2:1549735/1‑149 (MQ=255)
                                                                                                          gTTCGACACCGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTcaca                                            <  2:3744724/149‑1 (MQ=255)
                                                                                                          gTTCGAAAACGGCTTGACGTTCAGAAATTTAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTcaca                                            <  1:3823690/149‑1 (MQ=255)
                                                                                                           ttCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCacac                                           >  1:393060/1‑149 (MQ=255)
                                                                                                                 aaCGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATg                                     >  2:5230033/1‑149 (MQ=255)
                                                                                                                  aCGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATgc                                    <  2:2601329/149‑1 (MQ=255)
                                                                                                                  aCGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATgc                                    >  2:540933/1‑149 (MQ=255)
                                                                                                                     gCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATGCGcc                                 >  2:693552/1‑149 (MQ=255)
                                                                                                                      cATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATGCGccc                                <  2:780572/149‑1 (MQ=255)
                                                                                                                      cATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATGCGccc                                <  2:84416/149‑1 (MQ=255)
                                                                                                                        tGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATGCGCCCTg                              >  2:3479848/1‑149 (MQ=255)
                                                                                                                               cAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATGCGCCCTGGCCCGtt                       >  2:2515457/1‑149 (MQ=255)
                                                                                                                                aGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATGCGCCCTGGCCCGTTa                      >  1:2486626/1‑149 (MQ=255)
                                                                                                                                 gCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATGCGCCCTGGCCCGTTAt                     >  2:1611138/1‑149 (MQ=255)
                                                                                                                                   aaaTGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGAGTACGCTTCACACGCGATGCGCCCTGGCCCGTTATcc                   >  2:621235/1‑149 (MQ=255)
                                                                                                                                    aaTGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATGCGCCCTGGCCCGTTATCCa                  >  1:2928839/1‑149 (MQ=255)
                                                                                                                                       caaaGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATGCGCCCTGGCCCGTTATCCAGTc               <  1:1144971/148‑1 (MQ=255)
                                                                                                                                          aGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATGCGCCCTGGCCCGTTATCCAGTCAcc            >  1:3992571/1‑149 (MQ=255)
                                                                                                                                           gCCGCGCCCAGCGCGGACACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATGCGCCCTGGCCCGTTATCCAGTCACCg           <  1:1657452/149‑1 (MQ=255)
                                                                                                                                           gCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATGCGCCCTGGCCCGTTATCCAGTCACCg           >  1:3261201/1‑149 (MQ=255)
                                                                                                                                            ccGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATGCGCCCTGGCCCGTTATCCAGTCACCGt          <  1:1347772/149‑1 (MQ=255)
                                                                                                                                             cgcgCCCAGCGCGTAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATGCGCCCTGGCCCGTTATCCAGTCACCGtt         <  1:4258742/149‑1 (MQ=255)
                                                                                                                                             cgcgCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATGCGCCCTGGCCCGTTATCCAGTCACCGtt         >  1:1284208/1‑149 (MQ=255)
                                                                                                                                                gcCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATGCGCCCTGGCCCGTTATCCAGTCACCGTTAAt      <  2:2165400/149‑1 (MQ=255)
                                                                                                                                                gcCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATGCGCCCTGGCCCGTTATCCAGTCACCGTTAAt      >  1:3660481/1‑149 (MQ=255)
                                                                                                                                                 cccAGCGCGGACACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATGCGCCCTGGCCCGTTATCCAGTCACCGTTAATc     <  1:2089570/149‑1 (MQ=255)
                                                                                                                                                  ccAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATGCGCCCTGGCCCGTTATCCAGTCACCGTTAATcc    >  1:3884317/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
ATCATCGCCGTCACTTCGGTTGCCGCTTCCCAGCGCGGCTTACCCGTTTCTCTGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATGCGCCCTGGCCCGTTATCCAGTCACCGTTAATCCAT  >  CP009273/1823687‑1823983

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑