Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,195,449 A→T 91.6% E564D (GAA→GAT)  yehI → DUF4132 domain‑containing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,195,4490AT91.6% 967.8 / 59.0 274E564D (GAA→GAT) yehIDUF4132 domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base A (18/5);  new base T (125/126);  total (143/131)
Fisher's exact test for biased strand distribution p-value = 9.12e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01

Only 100 of 280 total aligned reads displayed.

GCTGCACGCAATTCACTCTTTGTTCAGTGGCATTACTACCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGAAAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCATCTGTTCGCGGGTGAGGACTATTTTCTCA  >  CP009273/2195303‑2195594
                                                                                                                                                  |                                                                                                                                                 
gctgcACGCAATTCACTCTTTGTTCAGTGGCATTACTACCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGataa                                                                                                                                                 >  1:3991152/1‑149 (MQ=255)
gctgcACGCAATTCACTCTTTGTTCAGTGGCATTACTACCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGataa                                                                                                                                                 >  1:2970085/1‑149 (MQ=255)
 ctgcACGCAATTCACTCTTTGTTCAGTGGCATTACTACCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGataag                                                                                                                                                <  2:2727258/149‑2 (MQ=255)
    cACGCAATTCACTCTTTGTTCAGTGGCATTACTACCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGAc                                                                                                                                             <  1:1872401/149‑1 (MQ=255)
      cGCAATTCACTCTTTGTTCAGTGGCATTACTACCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACaa                                                                                                                                           >  1:287954/1‑149 (MQ=255)
       gCAATTCACTCTTTGTTCAGTGGCATTACTACCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAac                                                                                                                                          >  2:3092234/1‑149 (MQ=255)
        cAATTCACTCTTTGTTCAGTGGCATTACTACCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAact                                                                                                                                         <  2:758079/149‑1 (MQ=255)
        cAATTCACTCTTTGTTCAGTGGCATTACTACCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAact                                                                                                                                         <  2:467404/149‑1 (MQ=255)
          aTTCACTCTTTGTTCAGTGGCATTACTACCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAactac                                                                                                                                       >  1:4673565/1‑149 (MQ=255)
           ttCACTCTTTGTTCAGTGGCATTACTACCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGAAAATGACAactact                                                                                                                                      <  2:1251304/149‑1 (MQ=255)
            tCACTCTTTGTTCAGTGGCATTACTACCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTc                                                                                                                                     <  2:2079677/149‑1 (MQ=255)
               ctctTTGTTCAGTGGCATTACTACCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTcaca                                                                                                                                  >  2:2203073/1‑149 (MQ=255)
               ctctTTGTTCAGTGGCATTACTACCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTcaca                                                                                                                                  >  2:350688/1‑149 (MQ=255)
               ctctTTGTTCAGTGGCATTACTACCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTcaca                                                                                                                                  <  1:1470496/149‑1 (MQ=255)
                tctTTGTTCAGTGGCATTACTACCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACAt                                                                                                                                 <  2:4354285/149‑1 (MQ=255)
                tctTTGTTCAGTCGCATTACTACCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACAt                                                                                                                                 >  2:402394/1‑149 (MQ=255)
                 ctTTGTTCAGTGGCATTACTACCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACAtt                                                                                                                                <  1:3647677/149‑1 (MQ=255)
                 ctTTGTTCAGTGGCATTACTACCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACAtt                                                                                                                                >  1:1017082/1‑149 (MQ=255)
                 ctTTGTTCAGTGGCATTACTACCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACAtt                                                                                                                                >  2:2514888/1‑149 (MQ=255)
                  tttGTTCAGTGGCATTACTACCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTa                                                                                                                               <  2:2332990/149‑1 (MQ=255)
                      ttCAGTGGCATTACTACCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGt                                                                                                                           >  1:632364/1‑149 (MQ=255)
                       tCAGTGGCATTACTACCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTc                                                                                                                          >  2:703706/1‑149 (MQ=255)
                        cAGTGGCATTACTACCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTcc                                                                                                                         >  2:4545141/1‑149 (MQ=255)
                           tGGCATTACTACCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGa                                                                                                                      >  2:2900179/1‑149 (MQ=255)
                            ggCATTACTACCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGaa                                                                                                                     >  1:2702111/1‑149 (MQ=255)
                              cATTACTACCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGaaaa                                                                                                                   <  2:3927654/149‑1 (MQ=255)
                              cATTACTACCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGaaaa                                                                                                                   >  2:4110063/1‑149 (MQ=255)
                              cATTACTACCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGaaaa                                                                                                                   >  2:90855/1‑149 (MQ=255)
                               aTTACTACCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAag                                                                                                                  >  2:2314310/1‑149 (MQ=255)
                                ttACTACCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAaga                                                                                                                 >  2:1159518/1‑149 (MQ=255)
                                   ctacCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAgtg                                                                                                              <  1:83692/149‑1 (MQ=255)
                                   ctacCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAgtg                                                                                                              >  2:331614/1‑149 (MQ=255)
                                    tacCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAgtgt                                                                                                             <  2:5156884/149‑1 (MQ=255)
                                      ccAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCt                                                                                                           <  2:296199/149‑1 (MQ=255)
                                       cAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTg                                                                                                          >  1:2279096/1‑149 (MQ=255)
                                       cAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATAAACATATGGGATAAAGACAACTACACAAAATATCGTCCGGAAAAGAGTGTCTg                                                                                                          >  1:2167942/1‑149 (MQ=255)
                                            gCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATTACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAAcc                                                                                                     <  1:1931603/149‑1 (MQ=255)
                                                 ttGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGtat                                                                                                <  2:1707119/149‑1 (MQ=255)
                                                 ttGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGtat                                                                                                <  2:4323392/149‑1 (MQ=255)
                                                   gAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGtattt                                                                                              >  1:2827240/1‑149 (MQ=255)
                                                     agagATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACAGATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGtatttat                                                                                            <  1:3477144/149‑1 (MQ=255)
                                                      gagaTCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGtatttatt                                                                                           >  2:891016/1‑149 (MQ=255)
                                                       agaTCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTg                                                                                          <  2:1264357/149‑1 (MQ=255)
                                                        gaTCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTgg                                                                                         <  2:3571677/149‑1 (MQ=255)
                                                         aTCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGc                                                                                        <  1:1078146/149‑1 (MQ=255)
                                                          tCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGAAAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGca                                                                                       >  1:3358326/1‑149 (MQ=255)
                                                              gAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGt                                                                                   >  1:2067627/1‑149 (MQ=255)
                                                              gAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGt                                                                                   <  1:321088/149‑1 (MQ=255)
                                                               aaTGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGtt                                                                                  <  2:3446725/149‑1 (MQ=255)
                                                               aaTGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGtt                                                                                  <  2:2779733/149‑1 (MQ=255)
                                                               aaTGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGtt                                                                                  >  1:2515751/1‑149 (MQ=255)
                                                                aTGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTg                                                                                 >  1:4253193/1‑149 (MQ=255)
                                                                 tGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGAAAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGc                                                                                <  1:553724/149‑1 (MQ=255)
                                                                    cTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCcgc                                                                             <  2:3320815/149‑1 (MQ=255)
                                                                    cTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACAGATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCcgc                                                                             <  1:4621756/149‑1 (MQ=255)
                                                                     tGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGAAAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCcgcg                                                                            >  2:338424/1‑149 (MQ=255)
                                                                       gAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTg                                                                          <  2:1341749/149‑1 (MQ=255)
                                                                         aCTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTgag                                                                        >  2:2388508/1‑149 (MQ=255)
                                                                          cTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTgaga                                                                       <  2:302474/149‑1 (MQ=255)
                                                                           tCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGAt                                                                      >  2:2494821/1‑149 (MQ=255)
                                                                             cccGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATgg                                                                    <  2:309821/149‑1 (MQ=255)
                                                                               cGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCt                                                                  <  2:3150169/149‑1 (MQ=255)
                                                                                        aaTTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTc                                                         <  1:898247/149‑1 (MQ=255)
                                                                                         aTTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTTACCTTCt                                                        <  1:3025672/149‑1 (MQ=255)
                                                                                         aTTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCt                                                        <  1:402394/149‑1 (MQ=255)
                                                                                             gCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCt                                                    <  2:1048119/149‑1 (MQ=255)
                                                                                               aGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGc                                                  >  1:4930431/1‑149 (MQ=255)
                                                                                               aGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGc                                                  <  1:3129397/149‑1 (MQ=255)
                                                                                                   cAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTAt                                              <  2:4840715/149‑1 (MQ=255)
                                                                                                       cgcTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAAt                                          <  1:1634913/149‑1 (MQ=255)
                                                                                                       cgcTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAAt                                          <  2:1922044/149‑1 (MQ=255)
                                                                                                        gcTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATg                                         >  2:559139/1‑149 (MQ=255)
                                                                                                                nnngTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAg                                 >  2:4169762/4‑149 (MQ=255)
                                                                                                                  ggTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCa                               <  1:958375/149‑1 (MQ=255)
                                                                                                                       caTAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCATCTGt                          >  2:1457094/1‑149 (MQ=255)
                                                                                                                       caTAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCATCTGt                          <  1:1518197/149‑1 (MQ=255)
                                                                                                                            aaGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCATCTGTTcgcg                     >  1:4593488/1‑149 (MQ=255)
                                                                                                                             aGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCATCTGTTCGCgg                    >  2:2143261/1‑149 (MQ=255)
                                                                                                                             aGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCATCTGTTCGCgg                    <  1:5154266/149‑1 (MQ=255)
                                                                                                                             aGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCATCTGTTCGCgg                    <  1:5034824/149‑1 (MQ=255)
                                                                                                                             aGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCATCTGTTCGCgg                    <  1:4771089/149‑1 (MQ=255)
                                                                                                                                gTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCATCTGTTCGCGGGTg                 >  1:1267364/1‑149 (MQ=255)
                                                                                                                                 ttaattaaCATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCATCTGTTCGCGGGTGa                >  2:4954341/1‑149 (MQ=255)
                                                                                                                                  taattaaCATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCATCTGTTCGCGGGTGAg               <  1:2316307/149‑1 (MQ=255)
                                                                                                                                   aattaaCATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCATCTGTTCGCGGGTGAgg              >  1:1227159/1‑149 (MQ=255)
                                                                                                                                   aattaaCATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCATCTGTTCGCGGGTGAgg              >  1:3845868/1‑149 (MQ=255)
                                                                                                                                   aattaaCATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCATCTGTTCGCGGGTGAgg              <  1:713494/149‑1 (MQ=255)
                                                                                                                                    attaaCATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCATCTGTTCGCGGGTGAGGa             >  1:882556/1‑149 (MQ=255)
                                                                                                                                    attaaCATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCATCTGTTCGCGGGTGAGGa             >  1:2559035/1‑149 (MQ=255)
                                                                                                                                        aCATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCATCTGTTCGCGGGTGAGGACTAt         <  2:1852521/149‑1 (MQ=255)
                                                                                                                                        aCATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCATCTGTTCGCGGGTGAGGACTAt         >  2:153582/1‑149 (MQ=255)
                                                                                                                                         cATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCATCTGTTCGCGGGTGAGGACTAtt        >  1:1715537/1‑149 (MQ=255)
                                                                                                                                          atatGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCATCTGTTCGCGGGTGAGGACTAttt       >  1:2251526/1‑149 (MQ=255)
                                                                                                                                           tatGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCATCTGTTCGCGGGTGAGGACTAtttt      >  2:1577142/1‑149 (MQ=255)
                                                                                                                                            atGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCATCTGTTCGCGGGTGAGGACTATTTtc     <  2:4481921/149‑1 (MQ=255)
                                                                                                                                            atGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCATCTGTTCGCGGGTGAGGACTATTTtc     <  1:2388508/149‑1 (MQ=255)
                                                                                                                                             tGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCATCTGTTCGCGGGTGAGGACTATTTtct    <  1:4406077/149‑1 (MQ=255)
                                                                                                                                              gggATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCATCTGTTCGCGGGTGAGGACTATTTtctc   >  2:4049699/1‑149 (MQ=255)
                                                                                                                                               ggATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCATTGAGAAAAAGCATCTGTTCGCGGGTGAGGACTATTTTCTCa  <  1:2900179/149‑1 (MQ=255)
                                                                                                                                               ggATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCATCTGTTCGCGGGTGAGGACTATTTTCTCa  >  2:3832850/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
GCTGCACGCAATTCACTCTTTGTTCAGTGGCATTACTACCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGAAAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCATCTGTTCGCGGGTGAGGACTATTTTCTCA  >  CP009273/2195303‑2195594

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑