Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,352,573 C→A 88.9% P353P (CCG→CCT)  rhmT ← putative L‑rhamnonate transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,352,5730CA88.9% 852.3 / 100.1 289P353P (CCG→CCT) rhmTputative L‑rhamnonate transporter
Reads supporting (aligned to +/- strand):  ref base C (18/14);  new base A (132/125);  total (150/139)
Fisher's exact test for biased strand distribution p-value = 7.08e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.83e-01

Only 100 of 290 total aligned reads displayed.

GCCAGCGTCAGTAATCCCGCCGCATCGCTGGCAAATAACGTTTCTGCTTTCACGCGCAGGATCGGGGCAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTCGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCATTACTGGAGAAAGCAGCCCGGACAGACCAATACCAATGCCCGCCGCCAGTAATGTCAGCGCTGCGACATTACGCCGTT  >  CP009273/2352427‑2352721
                                                                                                                                                  |                                                                                                                                                    
gCCAGCGTCAGTAATCCCGCCGCATCGCTGGCAAATAACGTTTCTGCTTTCACGCGCAGGATCGGGGCAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAgg                                                                                                                                                    <  1:1670817/149‑1 (MQ=255)
    gCGTCAGTAATCCCGCCGCATCGCTGGCAAATAACGTTTCTGCTTTCACGCGCAGGATCGGGGCAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTCGGCATc                                                                                                                                                >  2:2034775/1‑149 (MQ=255)
    gCGTCAGTAATCCCGCCGCATCGCTGGCAAATAACGTTTCTGCTTTCACGCGCAGGATCGGGGCAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATc                                                                                                                                                >  1:2171068/1‑149 (MQ=255)
    gCGTCAGTAATCCCGCCGCATCGCTGGCAAATAACGTTTCTGCTTTCACGCGCAGGATCGGGGCAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATc                                                                                                                                                <  2:3958552/149‑1 (MQ=255)
     cGTCAGTAATCCCGCCGCATCGCTGGCAAATAACGTTTCTGCTTTCACGCGCAGGATCGGGGCAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCg                                                                                                                                               <  1:3419544/149‑1 (MQ=255)
      gTCAGTAATCCCGCCGCATCGCTGGCAAATAACGTTTCTGCTTTCACGCGCAGGATCGGGGCAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGt                                                                                                                                              >  1:5082737/1‑149 (MQ=255)
       tCAGTAATCCCGCCGCATCGCTGGCAAATAACGTTTCTGCTTTCACGCGCAGGATCGGGGCAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTc                                                                                                                                             >  1:3605833/1‑149 (MQ=255)
        cAGTAATCCCGCCGCATCGCTGGCAAATAACGTTTCTGCTTTCACGCGCAGGATCGGGGCAATAAACCCGCCCACTGCACCAAACAGGTTTACAACACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTcc                                                                                                                                            <  2:3679185/149‑1 (MQ=255)
        cAGTAATCCCGCCGCATCGCTGGCAAATAACGTTTCTGCTTTCACGCGCAGGATCGGGGCAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTcc                                                                                                                                            >  2:1227680/1‑149 (MQ=255)
        cAGTAATCCCGCCGCATCGCTGGCAAATAACGTTTCTGCTTTCACGCGCAGGATCGGGGCAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTcc                                                                                                                                            <  2:3789704/149‑1 (MQ=255)
         aGTACTCCCGCCGCATCGCTGGCAAATAACGTTTCTGCTTTCACGCGCAGGATCGGGGCAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCa                                                                                                                                           <  2:1632153/149‑1 (MQ=255)
         aGTAATCCCGCCGCATCGCTGGCAAATAACGTTTCTGCTTTCACGCGCAGGATCGGGGCAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCa                                                                                                                                           <  2:775954/149‑1 (MQ=255)
          gTAATCCCGCCGCATCGCTGGCAAATAACGTTTCTGCTTTCACGCGCAGGATCGGGGCAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAg                                                                                                                                          >  2:3572605/1‑149 (MQ=255)
           tAATCCCGCCGCATCGCTGGCAAATAACGTTTCTGCTTTCACGCGCAGGATCGGGGCAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGa                                                                                                                                         <  2:4596621/149‑1 (MQ=255)
            aaTCCCGCCGCATCGCTGGCAAATAACGTTTCTGCTTTCACGCGCAGGATCGGGGCAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGaa                                                                                                                                        >  2:387362/1‑149 (MQ=255)
             aTCCCGCCGCATCGCTGGCAAATAACGTTTCTGCTTTCACGCGCAGGATCGGGGCAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAac                                                                                                                                       <  1:707526/149‑1 (MQ=255)
               cccGCCGCATCGCTGGCAAATAACGTTTCTGCTTTCACGCGCAGGATCGGGGCAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAacac                                                                                                                                     <  1:4117460/149‑1 (MQ=255)
                  gccgcATCGCTGGCAAATAACGTTTCTGCTTTCACGCGCAGGATCGGGGCAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTgg                                                                                                                                  >  2:1365666/1‑149 (MQ=255)
                     gcATCGCTGGCAAATAACGTTTCTGCTTTCACGCGCAGGATCGGGGCAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTg                                                                                                                               >  1:3833943/1‑149 (MQ=255)
                     gcATCGCTGGCAAATAACGTTTCTGCTTTCACGCGCAGGATCGGGGCAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTg                                                                                                                               >  2:1211665/1‑149 (MQ=255)
                     gcATCGCTGGCAAATAACGTTTCTGCTTTCACGCGCAGGATCGGGGCAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTg                                                                                                                               >  2:4675553/1‑149 (MQ=255)
                       aTCGCTGGCAAATAACGTTTCTGCTTTCACGCGCAGGATCGGGGCAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCa                                                                                                                             <  2:4495657/149‑1 (MQ=255)
                       aTCGCTGGCAAATAACGTTTCTGCTTTCACGCGCAGGATCGGGGCAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCa                                                                                                                             <  1:3128290/149‑1 (MQ=255)
                        tCGCTGGCAAATAACGTTTCTGCTTTCACGCGCAGGATCGGGGCAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCAc                                                                                                                            >  2:4790090/1‑149 (MQ=255)
                            tGGCAAATAACGTTTCTGCTTTCACGCGCAGGATCGGGGCAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCa                                                                                                                        <  1:4575599/149‑1 (MQ=255)
                            tGGCAAATAACGTTTCTGCTTTCACGCGCAGGATCGGGGCAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCa                                                                                                                        <  1:4328560/149‑1 (MQ=255)
                             ggCAAATAACGTTTCTGCTTTCACGCGCAGGATCGGGGCAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTCGGCATCGTCCAGAACACTGGCTGCACGGCaa                                                                                                                       <  2:372930/149‑1 (MQ=255)
                             ggCAAATAACGTTTCTGCTTTCACGCGCAGGATCGGGGCAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTCGGCATCGTCCAGAACACTGGCTGCACGGCaa                                                                                                                       <  2:2115966/149‑1 (MQ=255)
                              gCAAATAACGTTTCTGCTTTCACGCGCAGGATCGGGGCAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAAt                                                                                                                      <  1:1311684/149‑1 (MQ=255)
                                aaaTAACGTTTCTGCTTTCACGCGCAGGATCGGGGCAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATaa                                                                                                                    <  1:2210506/149‑1 (MQ=255)
                                aaaTAACGTTTCTGCTTTCACGCGCAGGATCGGGGCAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATaa                                                                                                                    <  1:4826731/149‑1 (MQ=255)
                                 aaTAACGTTTCTGCTTTCACGCGCAGGATCGGGGCAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATaaa                                                                                                                   <  2:1556329/149‑1 (MQ=255)
                                    aaCGTTTCTGCTTTCACGCGCAGGATCGGGGCAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAAccc                                                                                                                <  1:1157253/149‑1 (MQ=255)
                                       gTTTCTGCTTTCACGCGCAGGATCGGGGCAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGAt                                                                                                             >  1:374777/1‑149 (MQ=255)
                                        tttCTGCTTTCACGCGCAGGATCGGGGCAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATa                                                                                                            >  1:1873561/1‑149 (MQ=255)
                                          tCTGCTTTCACGCGCAGGATCGGGGCAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGc                                                                                                          >  1:1097611/1‑149 (MQ=255)
                                          tCTGCTTTCACGCGCAGGATCGGGGCAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGc                                                                                                          <  2:2145218/149‑1 (MQ=255)
                                           cTGCTTTCACGCGCAGGATCGGGGCAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCACTAAACCCGATAGCt                                                                                                         >  1:2445594/1‑149 (MQ=255)
                                           cTGCTTTCACGCGCAGGATCGGGGCAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCt                                                                                                         >  1:3229017/1‑149 (MQ=255)
                                           cTGCTTTCACGCGCAGGATCGGGGCAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCt                                                                                                         <  2:216734/149‑1 (MQ=255)
                                           cTGCTTTCACGCGCAGGATCGGGGCAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCt                                                                                                         <  2:2841260/149‑1 (MQ=255)
                                            tGCTTTCACGCGCAGGATCGGGGCAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTg                                                                                                        >  1:4435293/1‑149 (MQ=255)
                                            tGCTTTCACGCGCAGGATCGGGGCAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTg                                                                                                        >  1:2350696/1‑149 (MQ=255)
                                            tGCTTTCACGCGCAGGATCGGGGCAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTg                                                                                                        >  2:4742032/1‑149 (MQ=255)
                                             gCTTTCACGCGCAGGATCGGGGCAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGc                                                                                                       <  1:774369/149‑1 (MQ=255)
                                             gCTTTCACGCGCAGGATCGGGGCAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGc                                                                                                       >  1:4764008/1‑149 (MQ=255)
                                               tttCACGCGCAGGATCGGGGCAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCaa                                                                                                     <  1:1470117/149‑1 (MQ=255)
                                                ttCACGCGCAGGATCGGGGCAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAac                                                                                                    <  1:3937335/149‑1 (MQ=255)
                                                 tCACGCGCAGGATCGGGGCAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAaca                                                                                                   >  2:2550364/1‑149 (MQ=255)
                                                 tCACGCGCAGGATCGGGGCAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAaca                                                                                                   >  1:818591/1‑149 (MQ=255)
                                                 tCACGCGCAGGATCGGGGCAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAaca                                                                                                   <  1:1640974/149‑1 (MQ=255)
                                                 tCACGCGCAGGATCGGGGCAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAaca                                                                                                   >  2:3251973/1‑149 (MQ=255)
                                                  cACGCGCAGGATCGGGGCAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAacac                                                                                                  >  1:4202917/1‑149 (MQ=255)
                                                         aGGATCGGGGCAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAgcgc                                                                                           <  2:5063145/149‑1 (MQ=255)
                                                          ggATCGGGGCAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCt                                                                                          <  2:919974/149‑1 (MQ=255)
                                                           gATCGGGGCAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTa                                                                                         >  1:1952881/1‑149 (MQ=255)
                                                            aTCGGGGCAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTCGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTAc                                                                                        >  1:3980309/1‑149 (MQ=255)
                                                               ggggCAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGAt                                                                                     >  2:3609215/1‑149 (MQ=255)
                                                                gggCAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATc                                                                                    >  1:853984/1‑149 (MQ=255)
                                                                        aaCCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCATTAc                                                                            >  1:3525608/1‑149 (MQ=255)
                                                                        aaCCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCATTAc                                                                            >  2:843330/1‑149 (MQ=255)
                                                                          cccGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCATTACTg                                                                          <  1:2777542/149‑1 (MQ=255)
                                                                           ccGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCATTACTgg                                                                         >  2:4165231/1‑149 (MQ=255)
                                                                              cccACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCATTACTGgaga                                                                      <  1:493155/149‑1 (MQ=255)
                                                                               ccACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCATTACTGGAGaa                                                                     >  1:2188653/1‑149 (MQ=255)
                                                                               ccACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCATTACTGGAGaa                                                                     >  2:4460143/1‑149 (MQ=255)
                                                                                cACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCCTTACTGGAGaaa                                                                    >  2:5132528/1‑149 (MQ=255)
                                                                                cACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCATTACTGGAGaaa                                                                    >  1:104191/1‑149 (MQ=255)
                                                                                cACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCATTACTGGAGaaa                                                                    <  1:461733/149‑1 (MQ=255)
                                                                                cACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGAAGTTGTTAAGGCATCGTCCAGAAAACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCAATTACTGGAGaaa                                                                    >  2:5131716/1‑149 (MQ=255)
                                                                                 aCTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCATTACTGGAGAAag                                                                   >  1:1269926/1‑149 (MQ=255)
                                                                                  cTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCATTACTGGAGAAagc                                                                  <  2:1926354/149‑1 (MQ=255)
                                                                                      aCCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCATTACTGGAGAAAGCAGcc                                                              >  1:525694/1‑149 (MQ=255)
                                                                                       ccAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCATTACTGGAGAAAGCAGccc                                                             >  1:2623144/1‑149 (MQ=255)
                                                                                            cAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCATTACTGGAGAAAGCAGCCCGGACa                                                        >  2:1713938/1‑149 (MQ=255)
                                                                                                   aCAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCATTACTGGAGAAAGCAGCCCGGACAGaccaat                                                 <  1:1898876/149‑1 (MQ=255)
                                                                                                     aaaaCCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCATTACTGGAGAAAGCAGCCCGGACAGaccaatac                                               <  1:4675553/149‑1 (MQ=255)
                                                                                                      aaaCCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTCGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCATTACTGGAGAAAGCAGCCCGGACAGaccaatacc                                              >  1:2974506/1‑149 (MQ=255)
                                                                                                        aCCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTCGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCATTACTGGAGAAAGCAGCCCGGACAGaccaataccaa                                            <  2:3383872/149‑1 (MQ=255)
                                                                                                        aCCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCATTACTGGAGAAAGCAGCCCGGACAGaccaataccaa                                            <  1:2298927/149‑1 (MQ=255)
                                                                                                         ccAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTCGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCATTACTGGAGAAAGCAGCCCGGACAGaccaataccaat                                           <  2:1847742/149‑1 (MQ=255)
                                                                                                            aTTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCATTACTGGAGAAAGCAGCCCGGACAGACCAATACCAATGcc                                        >  2:4791946/1‑149 (MQ=255)
                                                                                                                     gccagcGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCATTACTGGAGAAAGCAGCCCGGACAGACCAATACCAATGCCCGCCGCCAg                               >  2:563538/1‑149 (MQ=255)
                                                                                                                     gccagcGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCATTACTGGAGAAAGCAGCCCGGACAGACCAATACCAATGCCCGCCGCCAg                               >  2:3187468/1‑149 (MQ=255)
                                                                                                                     gccagcGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCATTACTGGAGAAAGCAGCCCGGACAGACCAATACCAATGCCCGCCGCCAg                               <  1:2908633/149‑1 (MQ=255)
                                                                                                                     gccagcGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCATTACTGGAGAAAGCAGCCCGGACAGACCAATACCAATGCCCGCCGCCAg                               >  1:4470359/1‑149 (MQ=255)
                                                                                                                     gccagcGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCATTACTGGAGAAAGCAGCCCGGACAGACCAATACCAATGCCCGCCGCCAg                               >  1:54354/1‑149 (MQ=255)
                                                                                                                     gccagcGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCAGCAACACACAGCGCTACGATCGCCATTACTGGATAAAGCATCCCGGACAGACCAATACAAATGCCCGCCGCCAg                               >  1:3063596/1‑149 (MQ=255)
                                                                                                                         gcgcCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCATTACTGGAGAAAGCAGCCCGGACAGACCAATACCAATGCCCGCCGCCAGTAAt                           >  2:305218/1‑149 (MQ=255)
                                                                                                                         gcgcCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCATTACTGGAGAAAGCAGCCCGGACAGACCAATACCAATGCCCGCCGCCAGTAAt                           >  2:2930427/1‑149 (MQ=255)
                                                                                                                         gcgcCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCATTACTGGAGAAAGCAGCCCGGACAGACCAATACCAATGCCCGCCGCCAGTAAt                           <  2:635763/149‑1 (MQ=255)
                                                                                                                           gcCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCATTACTGGAGAAAGCAGCCCGGACAGACCAATACCAATGCCCGCCGCCAGTAATGt                         <  2:4956500/149‑1 (MQ=255)
                                                                                                                            ccGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCATTACTGGAGAAAGCAGCCCGGACAGACCAATACCAATGCCCGCCGCCAGTAATGTc                        >  2:4886986/1‑149 (MQ=255)
                                                                                                                            ccGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCATTACTGGAGAAAGCAGCCCGGACAGACCAATACCAATGCCCGCCGCCAGTAATGTc                        >  2:2269908/1‑149 (MQ=255)
                                                                                                                                   ggAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCATTACTGGAGAAAGCAGCCCGGACAGACCAATACCAATGCCCGCCGCCAGTAATGTCAGCGCTg                 >  1:5224462/1‑149 (MQ=255)
                                                                                                                                    gAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCATTACTGGAGAAAGCAGCCCGGACAGACCAATACCAATGCCCGCCGCCAGTAATGTCAGCGCTGc                >  1:1499644/1‑149 (MQ=255)
                                                                                                                                      aaGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCATTACTGGAGAAAGCAGCCCGGACAGACCAATACCAATGCCCGCCGCCAGTAATGTCAGCGCTGCGa              >  1:2863648/1‑149 (MQ=255)
                                                                                                                                      aaGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCATTACTGGAGAAAGCAGCCCGGACAGACCAATACCAATGCCCGCCGCCAGTAATGTCAGCGCTGCGa              >  2:2203070/1‑149 (MQ=255)
                                                                                                                                       aGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCATTACTGGAGAAAGCAGCCCGGACAGACCAATACCAATGCCCGCCGCCAGTAATGTCAGCGCTGCGAc             <  1:1751201/149‑1 (MQ=255)
                                                                                                                                            ttGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCATTACTGGAGAAAGCAGCCCGGACAGACCAATACCAATGCCCGCCGCCAGTAATGTCAGCGCTGCGACATTAc        <  2:238171/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                    
GCCAGCGTCAGTAATCCCGCCGCATCGCTGGCAAATAACGTTTCTGCTTTCACGCGCAGGATCGGGGCAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTCGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCATTACTGGAGAAAGCAGCCCGGACAGACCAATACCAATGCCCGCCGCCAGTAATGTCAGCGCTGCGACATTACGCCGTT  >  CP009273/2352427‑2352721

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑