Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,834,252 A→T 5.0% E457V (GAG→GTG)  ascF → fused cellobiose/arbutin/salicin‑specific PTS enzymes: IIB component/IC component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,834,2520AT 5.0% 1093.4 / 2.9 350E457V (GAG→GTG) ascFfused cellobiose/arbutin/salicin‑specific PTS enzymes: IIB component/IC component
Reads supporting (aligned to +/- strand):  ref base A (127/200);  new base T (18/3);  total (147/203)
Fisher's exact test for biased strand distribution p-value = 3.31e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 351 total aligned reads displayed.

TTGCCAGCCACTCAATGGCAGCGCCGGGGCTATTTACCAGCGTGCAGTTCTTCGATCCGGCGAATCCAATGAGCATCGTCTGGGTGTTCGCGGTCATGGCGCTGGCGGTGGTGCTGTCGTTTATCCTCACACTGTTGCTCGGCTTTGAGGATATTCCTGTTGAGGAAGCGGCTGCCCAGGCGCGAAAGTATCAGAGCGTACAACCGACCGTCGCCAAAGAAGTAAGTCTTAATTGAGGATGAAAATGTCAGTATTTCCAGAAAGTTTTTTATGGGGCGGCGCGCTTGCCGCCAACC  >  CP009273/2834105‑2834400
                                                                                                                                                   |                                                                                                                                                    
ttGCCAGCCACTCAATGGCAGCGCCGGGGCTATTTACCAGCGTGCAGTTCTTCGATCCGGCGAATCCAATGAGCATCGTCTGGGTGTTCGCGGTCATGGCGCTGGCGGTGGTGCTGTCGTTTATCCTCACACAGTTGCTCGGCGTTGAg                                                                                                                                                     >  1:4163371/1‑149 (MQ=255)
   ccagccaCTCAATGGCAGCGCCGGGGCTATTTACCAGCGTGCAGTTCTTCGATCCGGCGAATCCAATGAGCATCGTCTGGGTGTTCGCGGTCATGGCGCTGGCGGTGGTGCTGTCGTTTATCCTCACACTGTTGCTCGGCTTTGAGGat                                                                                                                                                  <  2:528378/149‑1 (MQ=255)
   ccagccaCTCAATGGCAGCGCCGGGGCTATTTACCAGCGTGCAGTTCTTCGATCCGGCGAATCCAATGAGCATCGTCTGGGTGTTCGCGGTCATGGCGCTGGCGGTGGTGCTGTCGTTTATCCTCACACAGTTGCTCGGCTTTGAGGat                                                                                                                                                  >  1:429413/1‑149 (MQ=255)
     agccaCTCAATGGCAGCGCCGGGGCTATTTACCAGCGTGCAGTTCTTCGATCCGGCGAATCCAATGAGCATCGTCTGGGTGTTCGCGGTCATGGCGCTGGCGGTGGTGCTGTCGTTTATCCTCACACTGTTGCTCGGCTTTGAGGatat                                                                                                                                                <  2:612242/149‑1 (MQ=255)
      gccaCTCAATGGCAGCGCCGGGGCTATTTACCAGCGTGCAGTTCTTCGATCCGGCGAATCCAATGAGCATCGTCTGGGTGTTCGCGGTCATGGCGCTGGCGGTGGTGCTGTCGTTTATCCTCACACTGTTGCTCGGCTTTGAGGATAtt                                                                                                                                               >  2:1929527/1‑149 (MQ=255)
      gccaCTCAATGGCAGCGCCGGGGCTATTTACCAGCGTGCAGTTCTTCGATCCGGCGAATCCAATGAGCATCGTCTGGGTGTTCGCGGTCATGGCGCTGGCGGTGGTGCTGTCGTTTATCCTCACACTGTTGCTCGGCTTTGAGGATAtt                                                                                                                                               <  2:2322813/149‑1 (MQ=255)
       ccaCTCAATGGCAGCGCCGGGGCTATTTACCAGCGTGCAGTTCTTCGATCCGGCGAATCCAATGAGCATCGTCTGGGTGTTCGCGGTCATGGCGCTGGCGGTGGTGCTGTCGTTTATCCTCACACTGTTGCTCGGCTTTGAGGATATTc                                                                                                                                              <  1:1234886/149‑1 (MQ=255)
        caCTCAATGGCAGCGCCGGGGCTATTTACCAGCGTGCAGTTCTTCGATCCGGCGAATCCAATGAGCATCGTCTGGGTGTTCGCGGTCATGGCGCTGGCGGTGGTGCTGTCGTTTATCCTCCCACTGTTGCTCGGCTTTGAGGATATTcc                                                                                                                                             >  2:2483010/1‑149 (MQ=255)
         aCTCAATGGCAGCGCCGGGGCTATTTACCAGCGTGCAGTTCTTCGATCCGGCGAATCCAATGAGCATCGTCTGGGTGTTCGCGGTCATGGCGCTGGCGGTGGTGCTGTCGTTTATCCTCACACTGTTGCTCGGCTTTGAGGATATTCCt                                                                                                                                            <  2:3184499/149‑1 (MQ=255)
          cTCAATGGCAGCGCCGGGGCTATTTACCAGCGTGCAGTTCTTCGATCCGGCGAATCCAATGAGCATCGTCTGGGTGTTCGCGGTCATGGCGCTGGCGGTGGTGCTGTCGTTTATCCTCACACTGTTGCTCGGCTTTGAGGATATTCCTg                                                                                                                                           <  1:645845/149‑1 (MQ=255)
          cTCAATGGCAGCGCCGGGGCTATTTACCAGCGTGCAGTTCTTCGATCCGGCGAATCCAATGAGCATCGTCTGGGTGTTCGCGGTCATGGCGCTGGCGGTGGTGCTGTCGTTTATCCTCACACTGTTGCTCGGCTTTGAGGATATTCCTg                                                                                                                                           <  1:2225805/149‑1 (MQ=255)
              aTGGCAGCGCCGGGGCTATTTACCAGCGTGCAGTTCTTCGATCCGGCGAATCCAATGAGCATCGTCTGGGTGTTCGCGGTCATGGCGCTGGCGGTGGTGCTGTCGTTTATCCTCACACTGTTGCTCGGCTTTGAGGATATTCCTGTTGa                                                                                                                                       <  2:3872199/149‑1 (MQ=255)
               tGGCAGCGCCGGGGCTATTTACCAGCGTGCAGTTCTTCGATCCGGCGAATCCAATGAGCATCGTCTGGGTGTTCGCGGTCATGGCGCTGGCGGTGGTGCTGTCGTTTATCCTCACACAGTTGCTCGGCTTTGAGGATATTCCTGTTGAg                                                                                                                                      >  2:4479034/1‑149 (MQ=255)
                ggCAGCGCCGGGGCTATTTACCAGCGTGCAGTTCTTCGATCCGGCGAATCCAATGAGCATCGTCTGGGTGTTCGCGGTCATGGCGCTGGCGGTGGTGCTGTCGTTTTTACACACACAGTTGCTCGGCGTTGTGGTTATTAAAGTTGAgg                                                                                                                                     >  1:4460623/1‑149 (MQ=255)
                  cAGCGCCGGGGCTATTTACCAGCGTGCAGTTCTTCGATCCGGCGAATCCAATGAGCATCGTCTGGGTGTTCGCGGTCATGGCGCTGGCGGTGGTGCTGTCGTTTATCCTCACACTGTTGCTCGGCTTTGAGGATATTCCTGTTGAGGaa                                                                                                                                   <  2:420693/149‑1 (MQ=255)
                   aGCGCCGGGGCTATTTACCAGCGTGCAGTTCTTCGATCCGGCGAATCCAATGAGCATCGTCTGGGTGTTCGCGGTCATGGCGCTGGCGGTGGTGCTGTCGTTTATCCTCACACTGTTGCTCGGCTTTGAGGATATTCCTGTTGAGGAAg                                                                                                                                  <  1:1245654/149‑1 (MQ=255)
                   aGCGCCGGGGCTATTTACCAGCGTGCAGTTCTTCGATCCGGCGAATCCAATGAGCATCGTCTGGGTGTTCGCGGTCATGGCGCTGGCGGTGGTGCTGTCGTTTATCCTCACACTGTTGCTCGGCTTTGAGGATATTCCTGTTGAGGAAg                                                                                                                                  <  1:3147499/149‑1 (MQ=255)
                     cgcCGGGGCTATTTACCAGCGTGCAGTTCTTCGATCCGGCGAATCCAATGAGCATCGTCTGGGTGTTCGCGGTCATGGCGCTGGCGGTGGTGCTGTCGTTTATCCTCACACTGTTGCTCGGCTTTGAGGATATTCCTGTTGAGGAAGCg                                                                                                                                <  2:4534336/149‑1 (MQ=255)
                        cGGGGCTATTTACCAGCGTGCAGTTCTTCGATCCGGCGAATCCAATGAGCATCGTCTGGGTGTTCGCGGTCATGGCGCTGGCGGTGGTGCTGTCGTTTATCCTCACACTGTTGCTCGGCTTTGAGGATATTCCTGTTGAGGAAGCGGCt                                                                                                                             <  2:1377312/149‑1 (MQ=255)
                         ggggCTATTTACCAGCGTGCAGTTCTTCGATCCGGCGAATCCAATGAGCATCGTCTGGGTGTTCGCGGTCATGGCGCTGGCGGTGGTGCTGTCGTTTATCCTCACACTGTTGCTCGGCTTTGAGGATATTCCTGTTGAGGAAGCGGCTg                                                                                                                            >  2:2547116/1‑149 (MQ=255)
                         ggggCTATTTACCAGCGTGCAGTTCTTCGATCCGGCGAATCCAATGAGCATCGTCTGGGTGTTCGCGGTCATGGCGCTGGCGGTGGTGCTGTCGTTTATCCTCACACTGTTGCTCGGCTTTGAGGATATTCCTGTTGAGGAAGCGGCTg                                                                                                                            >  2:3294073/1‑149 (MQ=255)
                           ggCTATTTACCAGCGTGCAGTTCTTCGATCCGGCGAATCCAATGAGCATCGTCTGGGTGTTCGCGGTCATGGCGCTGGCGGTGGTGCTGTCGTTTATCCTCACACAGTTTCTCGGCTTTTTGGATATTACTGTTGAGGAAGCGGCTGcc                                                                                                                          >  1:152824/1‑149 (MQ=255)
                            gCTATTTACCAGCGTGCAGTTCTTCGATCCGGCGAATCCAATGAGCATCGTCTGGGTGTTCGCGGTCATGGCGCTGGCGGTGGTGCTGTCGTTTATACTCACACTGTTGCTCGGCTTTGAGGATATTCCTGTTGAGGAAGCGGCTGccc                                                                                                                         >  2:1704166/1‑149 (MQ=255)
                            gCTATTTACCAGCGTGCAGTTCTTCGATCCGGCGAATCCAATGAGCATCGTCTGGGTGTTCGCGGTCATGGCGCTGGCGGTGGTGCTGTCGTTTATACTCACACAGTTGCTCGGCGTTGAGGATATTACTGTTGTGGAAGCGGCTGccc                                                                                                                         >  1:4799260/1‑149 (MQ=255)
                             cTATTTACCAGCGTGCAGTTCTTCGATCCGGCGAATCCAATGAGCATCGTCTGGGTGTTCGCGGTCATGGCGCTGGCGGTGGTGCTGTCGTTTATCCTCACACTGTTGCTCGGCTTTGAGGATATTCCTGTTGAGGAAGCGGCTGCCCa                                                                                                                        <  1:3587169/149‑1 (MQ=255)
                                      aGCGTGCAGTTCTTCGATCCGGCGAATCCAATGAGCATCGTCTGGGTGTTCGCGGTCAGGGCGCTGGCGGTGGTGCTGTCGTTTATCCTCACACTGTTGCTCGGCTTTGAGGATATTCCTGTTGAGGAAGCGGCTGCCCAGGCGCGaaa                                                                                                               <  1:9380/149‑1 (MQ=255)
                                       gCGTGCAGTTCTTCGATCCGGCGAATCCAATGAGCATCGTCTGGGTGTTCGCGGTCATGGCGCTGGCGGTGGTGCTGTCGTTTATCCTCACACTGTTGCTCGGCTTTGAGGATATTCCTGTTGAGGAAGCGGCTGCCCAGGCGCGAAAg                                                                                                              >  2:5126250/1‑149 (MQ=255)
                                       gCGTGCAGTTCTTCGATCCGGCGAATCCAATGAGCATCGTCTGGGTGTTCGCGGTCATGGCGCTGGCGGTGGTGCTGTCGTTTATCCTCACACAGTTGCTCGGCGTTGAGGATATTACTGTTGAGGAAGCGGCTGCCCCGGCGCGAAAg                                                                                                              >  1:1609571/1‑149 (MQ=255)
                                         gTGCAGTTCTTCGATCCGGCGAATCCAATGAGCATCGTCTGGGTGTTCGCGGTCATGGCGCTGGCGGTGGTGCTGTCGTTTATCCTCACACTGTTGCTCGGCTTTGAGGATATTCCTGTTGAGGAAGCGGCTGCCCAGGCGCGAAAGTa                                                                                                            >  2:4252100/1‑149 (MQ=255)
                                         gTGCAGTTCTTCGATCCGGCGAATCCAATGAGCATCGTCTGGGTGTTCGCGGTCATGGCGCTGGCGGTGGTGCTGTCGTTTATCCTCACACTGTTGCTCGGCTTTGAGGATATTCCTGTTGAGGAAGCGGCTGCCCAGGCGCGAAAGTa                                                                                                            <  2:5198256/149‑1 (MQ=255)
                                         gTGCAGTTCTTCGATCCGGCGAATCCAATGAGCATCGTCTGGGTGTTCGCGGTCATGGCGCTGGCGGTGGTGCTGTCGTTTATCCTCACACTGTTGCTCGGCTTTGAGGATATTACTGTTGAGGAAGCGGCTGCCCAGGCGCGAAAGTa                                                                                                            >  2:4028208/1‑149 (MQ=255)
                                            cAGTTCTTCGATCCGGCGAATCCAATGAGCATCGTCTGGGTGTTCGCGGTCATGGCGCTGGCGGTGGTGCTGTCGTTTATCCTCACACTGTTGCTCGGCTTTGAGGATATTCCTGTTGAGGAAGCGGCTGCCCAGGCGCGAAAGTATCa                                                                                                         <  1:1088213/149‑1 (MQ=255)
                                            cAGTTCTTCGATCCGGCGAATCCAATGAGCATCGTCTGGGTGTTCGCGGTCATGGCGCTGGCGGTGGTGCTGTCGTTTATACTCACAATGTTGCTCGGCGTTGAGGATATTCCTGTTGAGGAAGCGGCTGCCCAGGCGCGAAAGTATCa                                                                                                         >  2:4934736/1‑149 (MQ=255)
                                              gTTCTTCGATCCGGCGAATCCAATGAGCATCGTCTGGGTGTTCGCGGTCATGGCGCTGGCGGTGGTGCTGTCGTTTATCCTCACACTGTTGCTCGGCTTTGAGGATATTCCTGTTGAGGAAGCGGCTGCCCAGGCGCGAAAGTATCaga                                                                                                       >  2:4734356/1‑149 (MQ=255)
                                               ttcttcGATCCGGCGAATCCAATGAGCATCGTCTGGGTGTTCGCGGTCATGGCGCTGGCGGTGGTGCTGTCGTTTATCCTCACACTGTTGCTCGGCTTTGAGGATATTCCTGTTGAGGAAGCGGCTGCCCAGGCGCGAAAGTATCagag                                                                                                      <  2:2413826/149‑1 (MQ=255)
                                               ttcttcGATCCGGCGAATCCAATGAGCATCGTCTGGGTGTTCGCGGTCATGGCGCTGGCGGTGGTGCTGTCGTTTATCCTCACACTGTTGCTCGGCTTTGAGGATATTCCTGTTGAGGAAGCGGCTGCCCAGGCGCGAAAGTATCagag                                                                                                      <  2:2920660/149‑1 (MQ=255)
                                               ttcttcGATCCGGCGAATCCAATGAGCATCGTCTGGGTGTTCGCGGTCATGGCGCTGGCGGTGGTGCTGTCGTTTATCCTCACACTGTTGCTCGGCTTTGAGGATATTCCTGTTGAGGAAGCGGCTGCCCAGGCGCGAAAGTATCagag                                                                                                      <  1:1647704/149‑1 (MQ=255)
                                                   tcGATCCGGCGAATCCAATGAGCATCGTCTGGGTGTTCGCGGTCATGGCGCTGGCGGTGGTGCTGTCGTTTATCCTCACACTGTTGCTCGGCTTTGAGGATATTCCTGTTGAGGAAGCGGCTGCCCAGGCGCGAAAGTATCAGAGCGTa                                                                                                  <  2:3523976/149‑1 (MQ=255)
                                                      aTCCGGCGAATCCAATGAGCATCGTCTGGGTGTTCGCGGTCATGGCGCTGGCGGTGGTGCTGTCGTTTATCCTCACACTGTTGCTCGGCTTTGAGGATATTCCTGTTGAGGAAGCGGCTGCCCAGGCGCGAAAGTATCAGAGCGTACaa                                                                                               >  2:1178937/1‑149 (MQ=255)
                                                      aTCCGGCGAATCCAATGAGCATCGTCTGGGTGTTCGCGGTCATGGCGCTGGCGGTGGTGCTGTCGTTTATCCACACACAGTTGCTCGGCGTTGAGGATATTCCAGTTGTGGGATCGGCTGCCCAGGCGCGAAAGTATCAGAGCGTACaa                                                                                               >  1:3855758/1‑149 (MQ=255)
                                                      aTCCGGCGAATCCAATGAGCATCGTCTGGGTGTTCGCGGTCATGGCGCTGGCGGTGGTGCTGTCGTGTTTACTCACACTGTTGCTCGGCGTTGTGGTTAATCCGGTTTATGAAGCGGCTGCCCCGGCGCGAAAGAATAAGAGCGTACaa                                                                                               >  1:2367859/1‑149 (MQ=255)
                                                        ccGGCGAATCCAAGGAGCATCGTCTGGGTGTTCGCGGTCATGGCGCTGGCGGTGGTGCTGTCGTTTATCCTCACACTGTTGCTCGGCTTTGAGGATATTCCTGTTGAGGAAGCGGCTGCCCAGGCGCGAAAGTATCAGAGCGTACAacc                                                                                             <  2:2742272/149‑1 (MQ=255)
                                                         cGGCGAATCCAATGAGCATCGTCTGGGTGTTCGCGGTCATGGCGCTGGCGGTGGTGCTGTCGTTTATCCTCACACTGTTGCTCGGCTTTGAGGATATTCCTGTTGAGGAAGCGGCTGCCCAGGCGCGAAAGTATCAGAGCGTACAaccg                                                                                            >  2:4571044/1‑149 (MQ=255)
                                                              aaTCCAATGAGCATCGTCTGGGTGTTCGCGGTCATGGCGCTGGCGGTGGTGCTGTCGTTTATCCTCACACTGTTGCTCGGCTTTGAGGATATTCCTGTTGAGGAAGCGGCTGCCCAGGCGCGAAAGTATCAGAGCGGACAACCGACCGt                                                                                       >  2:3731484/1‑149 (MQ=255)
                                                              aaTCCAATGAGCATCGTCTGGGTGTTCGCGGTCATGGCGCTGGCGGTGGTGCTGTCGTTTATCCTCACACTGTTGCTCGGCTTTGAGGATATTCCGGTTGAGGAAGCGGCTGCCCACGCGCGCAAGTAACAGAGCGTACAACCGACCGt                                                                                       >  1:4250341/1‑149 (MQ=255)
                                                              aaTCCAATGAGCATCGTCTGGGTGTTCGCGGTCATGGCGCTGGCGGTGGTGCTGTCGTGTATCCGCACCCTGGTGCTCGGCGTTGAGGATATTCCGGGTGAGGAAGCGGCAGCCCACGCGCGAAAGTATCAGAGCCTACCaccgcccct                                                                                       >  2:780248/1‑147 (MQ=255)
                                                                  cAATGAGCATCGTCTGGGTGTTCGCGGTCATGGCGCTGGCGGTGGTGCTGTCGTTTATACTCACACTGTTGCTCGGCTTTGAGGATATTCCTGTTGAGGAAGCGGCTGCCCAGGCGCGAAAGTATCAGAGCGTACAACCGACCGTCGcc                                                                                   >  2:2862865/1‑149 (MQ=255)
                                                                   aaTGAGCATCGTCTGGGTGTTCGCGGTCATGGCGCTGGCGGTGGTGCTGTCGTTTATACACACACAGTTTCTCGGCTTTTTTGATATTAATGTTTTGGTAGCGGCTGCCCAGGCGCCAAAGTATAAGAGCGTACAAACGACCCTCGCCa                                                                                  >  1:1555249/1‑149 (MQ=255)
                                                                      gAGCATCGTCTGGGTGTTCGCGGTCATGGCGCTGGCGGTGGTGCTGTCGTTTATCCTCACACTGTTGCTCGGCTTTGAGGATATTCCTGTTGAGGAAGCGGCTGCCCAGGCGCGAAAGTATCAGAGCGTACAACCGACCGTCGCCAaag                                                                               <  2:478932/149‑1 (MQ=255)
                                                                       aGCATCGTCTGGGTGTTCGCGGTCATGGCGCTGGCGGTGGTGCTGTCGTTTATCCTCACACTGTTGCTCGGCTTTGAGGATATTCCTGTTGAGGAAGCGGCTGCCCAGGCGCGAAAGTATCAGAGCGTACAACCGACCGTCGCCAaaga                                                                              <  2:2082695/149‑1 (MQ=255)
                                                                        gCATCGTCTGGGTGTTCGCGGTCATGGCGCTGGCGGTGGTGCTGTCGTTTATCCTCACACTGTTGCTCGGCTTTGAGGATATTCCTGTTGAGGAAGCGGCTGCCCAGGCGCGAAAGTATCAGAGCGTACAACCGACCGTCGCCAaagaa                                                                             >  2:428267/1‑149 (MQ=255)
                                                                         cATCGTCTGGGTGTTCGCGGTCATGGCGGTGGCGGTGGTGCTGTCGTTTATCCTCACACTGTTGCTCGGCTTTGAGGATATTCCTGTTGAGGAAGCGGCTGCCCAGGCGCGAAAGTATCAGAGCGTACAACCGACCGTCGCCAaagaag                                                                            <  1:3574481/149‑1 (MQ=255)
                                                                         cATCGTCTGGGTGTTCGCGGTCATGGCGCTGGCGGTGGTGCTGTCGTTTATCCTCACACTGTTGCTCGGCTTTGAGGATATTCCTGTTGAGGAAGCGGCTGCCCAGGCGCGAAAGTATCAGAGCGTACAACCGACCGTCGCCAaagaag                                                                            <  1:1292127/149‑1 (MQ=255)
                                                                           tCGTCTGGGTGTTCGCGGTCATGGCGCTGGCGGTGGTGCTGTCGTTTATACACACACAGTTTCTAGGCGTTGTGGATAATGCTGTTGAGGAAGCGGCTGCCCAGGCGCGAAAGTATAAGAGCGTACAAACCACCCTCGCCAAAGaaata                                                                          >  1:1143818/1‑149 (MQ=255)
                                                                            cGTCTGGGTGTTCGCGGTCATGGCGCTGGCGGTGGTGCTGTCGTTTATCCTCACACTGTTGCTCGGCTTTGAGGATATTCCTGTTGAGGAAGCGGCTGCCCAGGCGCGAAAGTATCAGAGCGTACAACCGACCGTCGCCAAAGaagtaa                                                                         <  1:3359653/149‑1 (MQ=255)
                                                                                 gggTGTTCGCGGTCATGGCGCTGGCGGTGGTGCTGTCGTTTATCCTCACACTGTTGCTCGGCTTTGAGGATATTCCTGTTGAGGAAGCGGCTGCCCAGGCGCGAAAGTATCAGAGCGTACAACCGACCGTCGCCAAAGAAGTAAGTCtt                                                                    <  2:4389670/149‑1 (MQ=255)
                                                                                 gggTGTTCGCGGTCATGGCGCTGGCGGTGGTGCTGTCGTTTATCCTCACACTGTTGCTCGGCTTTGAGGATATTCCTGTTGAGGAAGCGGCTGCCCAGGCGCGAAAGTATCAGAGCGTACAACCGACCGTCGCCAAAGAAGTAAGTCtt                                                                    <  1:1827875/149‑1 (MQ=255)
                                                                                  ggTGTTCGCGGTCATGGCGCTGGCGGTGGTGCTGTCGTTTATCCTCACACTGTTGCTCGGCTTTGAGGATATTCCTGTTGAGGAAGCGGCTGCCCAGGCGCGAAAGTATCAGAGCGTACAACCGACCGTCGCCAAAGAAGTAAGTCTTa                                                                   <  2:941810/149‑1 (MQ=255)
                                                                                  ggTGTTCGCGGTCATGGCGCTGGCGGTGGTGCTGTCGTTTATACTCACACAGTTGCTCGGCTTTGAGGATAATACGGTTGAGGAAGCGGCTGCCCAGGCGCGAAATTATCAGAGCGGACAACCGACCGTCGCCCAAGAAGTAAGTCTTa                                                                   >  1:347841/1‑149 (MQ=255)
                                                                                    tgtTCGCGGTCATGGCGCTGGCGGTGGTGCTGTCGTTTATCCTCACACTGTTGCTCGGCTTTGAGGATATTCCTGTTGAGGAAGCGGCTGCCCAGGCGCGAAAGTATCAGAGCGTACAACCGACCGTCGCCAAAGAAGTAAGTCTTAAt                                                                 <  1:1306529/149‑1 (MQ=255)
                                                                                    tgtTCGCGGTCATGGCGCTGGCGGTGGTGCTGTCGTTTATCCTCACACTGTTGCTCGGCTTTGAGGATATTCCTGTTGAGGAAGCGGCTGCCCAGGCGCGAAAGTATCAGAGCGTACAACCGACCGTCGCCAAAGAAGTAAGTCTTAAt                                                                 <  1:3914145/149‑1 (MQ=255)
                                                                                     gtTCGCGGTCATGGCGCTGGCGGTGGTGCTGTCGTTTATCCTCACACTGTTGCTCGGCTTTGAGGATATTCCTGTTGAGGAAGCGGCTGCCCCGGCGCGAAAGTATCAGAGCGTACAACCGACCGTCGCCAAAGAAGTAAGTCTTAAtt                                                                >  2:1185783/1‑149 (MQ=255)
                                                                                     gtTCGCGGTCATGGCGCTGGCGGTGGTGCTGTCGTTTATCCTCACACTGTTGCTCGGCTTTGAGGATATTCCTGTTGAGGAAGCGGCTGCCCAGGCGCGAAAGTATCAGAGCGTACAACCGACCGTCGCCAAAGAAGTAAGTCTTAAtt                                                                <  1:2484463/149‑1 (MQ=255)
                                                                                     gtTCGCGGTCATGGCGCTGGCGGTGGTGCTGTCGTTTATCCTCACACTGTTGCTCGGCGTTGAGGATAATCCTGTTGAGGAAGCGGCTGCCCAGGCGCGAAAGTATCAGATCGTACAACCGACCCTCGCCAAAGAAGTAATTCATAAtt                                                                >  2:3804664/1‑149 (MQ=255)
                                                                                      ttCGCGGTCATGGCGCTGGCGGTGGTGCTGTCGTTTATACTCACACGGTTTCTCGGCGTTGAGGATATTACTGTTGAGGAAGCGGCTGGCCAGGCGCGAAAGTATCAGAGCGTACAACCCACCGTCGCCAAAGAAGTAAGTCTGAAGTg                                                               >  1:4357807/1‑149 (MQ=255)
                                                                                      ttCGCGGTCATGGCGCTGGCGGTGGTGCTGTCGTGTATACACACACAGTTGGTCGGGGTTGTGGATATTGCTGTTGAGGGAGCGGCTGCCCCGGCGCGAAATTATAAGCGCGGACAACCGCCCGTCGCCAAAGAAGTAAGTGGTAAttt                                                               >  2:2218381/1‑148 (MQ=255)
                                                                                         gcgGTCATGGCGCTGGCGGTGGTGCTGTCGTTTATCCTCACACTGTTGCTCGGCTTTGAGGATATTCCTGTTGAGGAAGCGGCTGCCCAGGCGCGAAAGTATCAGAGCGTACAACCGACCGTCGCCAAAGAAGTAAGTCTTAATTGTgg                                                            >  2:370796/1‑149 (MQ=255)
                                                                                          cgGTCATGGCGCTGGCGGTGGTGCTGTCGTTTATCCTCACACTGTTGCTCGGCTTTGAGGATATTCCTGTTGAGGAAGCGGCTGCCCAGGCGCGAAAGTATCAGAGCGTACAACCGACCGTCGCCAAAGAAGTAAGTCTTAATTGAGGa                                                           <  2:1987117/149‑1 (MQ=255)
                                                                                           ggTCATGGCGCTGGCGGTGGTGCTGTCGTTTATCCTCACACTGTTGCTCGGCTTTGAGGATATTCCTGTTGAGGAAGCGGCTGCCCAGGCGCGAAAGTATCAGAGCGTACAACCGACCGTCGCCAAAGAAGTAAGTCTTAATTGAGGAt                                                          <  1:228481/149‑1 (MQ=255)
                                                                                                  gcgcTGGCGGTGGTGCTGTCGTTTATCCTCACACTGTTGCTCGGCTTTGAGGATATTCCTGTTGAGGAAGCGGCTGCCCAGGCGCGAAAGTATCAGAGCGTACAACCGACCGTCGCCAAAGAAGTAAGTCTTAATTGAGGATGAAAATg                                                   <  2:1080339/149‑1 (MQ=255)
                                                                                                    gcTGGCGGTGGTGCTGTCGTTTATCCTCACACTGTTGCTCGGCTTTGAGGATATTCCTGTTGAGGAAGCGGCTGCCCAGGCGCGAAAGTATCAGAGCGTACAACCGACCGTCGCCAAAGAAGTAAGTCTTAATTGAGGATGAAAATGTc                                                 <  2:3593163/149‑1 (MQ=255)
                                                                                                       ggCTGTGGTGCTGTCGTTTATCCTCACTCTGTTGCTCGGCTTTGTGGTTATTCCTGTTGAGTAAGCGGCTGCCCAGGCGCTAAAGTATCTGAGCTTACAACCGACCGTCGCCAAAGAAGTAAGTGTTAATTGAGGTTGAAAATGTCAGt                                              <  1:2756158/149‑1 (MQ=255)
                                                                                                       ggCGGTGGTGCTGTCGTTTATCCTCACACTGTTGCTCGGCTTTGAGGATATTCCTGTTGAGGAAGCGGCTGCCCAGGCGCGAAAGTATCAGAGCGTACAACCGACCGTCGCCAAAGAAGTAAGTCTTAATTGAGGATGAAAATGTCAGt                                              <  2:3634751/149‑1 (MQ=255)
                                                                                                       ggCGGTGGTGCTGTCGTTTATCCTCACACTGTTGCTCGGCTTTGAGGATATTCCTGTTGAGGAAGCGGCTGCCCAGGCGCGAAAGTATCAGAGCGTACAACCGACCGTCGCCAAAGAAGTAAGTCTTAATTGAGGATGAAAATGTCAGt                                              <  1:3692608/149‑1 (MQ=255)
                                                                                                         cGGTGGTGCTGTCGTTTATCCTCACACTGTTGCTCGGCTTTGAGGATATTCCTGTTGAGGAAGCGGCTGCCCAGGCGCGAAAGTATCAGAGCGTACAACCGACCGTCGCCAAAGAAGTAAGTCTTAATTGAGGATGAAAATGTCAGTAt                                            <  1:3975407/149‑1 (MQ=255)
                                                                                                             ggtgCTGTCGTTTATCCTCACACTGTTGCTCGGCTTTGAGGATATTCCTGTTGAGGAAGCGGCTGCCCAGGCGCGAAAGTATCAGAGCGTACAACCGACCGTCGCCAAAGAAGTAAGTCTTAATTGAGGATGAAAATGTCAGTATTTcc                                        <  2:4701602/149‑1 (MQ=255)
                                                                                                               tgCTGTCGTTTATCCTCACACTGTTGCTCGGCTTTGAGGATATTCCTGTTGAGGAAGCGGCTGCCCAGGCGCGAAAGTATCAGAGCGTACAACCGACCGTCGCCAAAGAAGTAAGTCTTAATTGAGGATGAAAATGTCAGTATTTCCAg                                      <  2:1291424/149‑1 (MQ=255)
                                                                                                               tgCTGTCGTTTATCCTCACACTGTTGCTCGGCTTTGAGGATATTCCTGTTGAGGAAGCGGCTGCCCAGGCGCGAAAGTATCAGAGCGTACAACCGACCGTCGCCAAAGAAGTAAGTCTTAATTGAGGATGAAAATGTCAGTATTTCCAg                                      <  2:699442/149‑1 (MQ=255)
                                                                                                                gCTGTCGTTTATCCTCACACTGTTGCTCGGCTTTGAGGATATTCCTGTTGAGGAAGCGGCTGCCCAGGCGCGAAAGTATCAGAGCGTACAACCGACCGTCGCCAAAGAAGTAAGTCTTAATTGAGGATGAAAATGTCAGTATTTCCAGa                                     <  2:1734083/149‑1 (MQ=255)
                                                                                                                gCTGTCGTTTATCCTCACACTGTTGCTCGGCTTTGAGGATATTCCTGTTGAGGAAGCGGCTGCCCAGGCGCGAAAGTATCAGAGCGTACAACCGACCGTCGCCAAAGAAGTAAGTCTTAATTGAGGATGAAAATGTCAGTATTTCCAGa                                     <  2:154331/149‑1 (MQ=255)
                                                                                                                   gTCGTTTATCCTCACACTGTTGCTCGGCTTTGAGGATATTCCTGTTGAGGAAGCGGCTGCCCAGGCGCGAAAGTATCAGAGCGTACAACCGACCGTCGCCAAAGAAGTAAGTCTTAATTGAGGATGAAAATGTCAGTATTTCCAGAAAg                                  <  2:877005/149‑1 (MQ=255)
                                                                                                                   gTCGTTTATCCTCACACTGTTGCTCGGCTTTGAGGATATTCCTGTTGAGGAAGCGGCTGCCCAGGCGCGAAAGTATCAGAGCGTACAACCGACCGTCGCCAAAGAAGTAAGTCTTAATTGAGGATGAAAATGTCAGTATTTCCAGAAAg                                  <  2:1458669/149‑1 (MQ=255)
                                                                                                                   gTCGTTTATCCTCACACTGTTGCTCGGCTTTGAGGATATTCCTGTTGAGGAAGCGGCTGCCCAGGCGCGAAAGTATCAGAGCGTACAACCGACCGTCGCCAAAGAAGTAAGTCTTAATTGAGGATGAAAATGTCAGTATTTCCAGAAAg                                  >  2:547110/1‑149 (MQ=255)
                                                                                                                    tCGTTTATCCTCCCACTGTTGCTCGGCTTTGAGGATATTCCTGTTGAGGAAGCGGCTGCCCAGGCGCGAAAGTATCAGAGCGTACAACCGACCGTCGCCAAAGAAGTAAGTCTTAATTGAGGATGAAAATGTCAGTATTTCCAGAAAGt                                 <  2:1623278/149‑1 (MQ=255)
                                                                                                                    tCGTTTATCCTCACACTGTTGCTCGGCTTTGAGGATATTCCTGTTGAGGAAGCGGCTGCCCAGGCGCGAAAGTATCAGAGCGTACAACCGACCGTCGCCAAAGAAGTAAGTCTTAATTGAGGATGAAAATGTCAGTATTTCCAGAAAGt                                 <  2:3970336/149‑1 (MQ=255)
                                                                                                                    tCGTTTATCCTCACACTGTTGCTCGGCTTTGAGGATATTCCTGTTGAGGAAGCGGCTGCCCAGGCGCGAAAGTATCAGAGCGTACAACCGACCGTCGCCAAAGAAGTAAGTCTTAATTGAGGATGAAAATGTCAGTATTTCCAGAAAGt                                 <  2:4173247/149‑1 (MQ=255)
                                                                                                                     cGTTTATCCTCACACTGTTGCTCGGCTTTGAGGATATTCCTGTTGAGGAAGCGGCTGCCCAGGCGCGAAAGTATCAGAGCGTACAACCGACCGTCGCCAAAGAAGTAAGTCTTAATTGAGGATGAAAATGTCAGTATTTCCAGAAAGtt                                <  1:895688/149‑1 (MQ=255)
                                                                                                                         tATCCTCACACTGTTGCTCGGCTTTGAGGATATTCCTGTTGAGGAAGCGGCTGCCCAGGCGCGAAAGTATCAGAGCGTACAACCGACCGTCGCCAAAGAAGTAAGTCTTAATTGAGGATGAAAATGTCAGTATTTCCAGAAAGtttttt                            >  1:322112/1‑149 (MQ=255)
                                                                                                                          aTCCTCACACTGTTGCTCGGCTTTGAGGATATTCCTGTTGAGGAAGCGGCTGCCCAGGCGCGAAAGTATCAGAGCGTACAACCGACCGTCGCCAAAGAAGTAAGTCTTAATTGAGGATGAAAATGTCAGTATTTCCAGAAAGTTTTTTa                           <  1:3450836/149‑1 (MQ=255)
                                                                                                                           tCCTCACACTGTTGCTCGGCTTTGAGGATATTCCTGTTGAGGAAGCGGCTGCCCAGGCGCGAAAGTATCAGAGCGTACAACCGACCGTCGCCAAAGAAGTAAGTCTTAATTGAGGATGAAAATGTCAGTATTTCCAGAAAGTTTTTTAt                          >  1:269007/1‑149 (MQ=255)
                                                                                                                             cTCACACTGTTGCTCGGCTTTGAGGATATTCCTGTTGAGGAAGCGGCTGCCCAGGCGCGAAAGTATCAGAGCGTACAACCGACCGTCGCCAAAGAAGTAAGTCTTAATTGAGGATGAAAATGTCAGTATTTCCAGAAAGTTTTTTATgg                        >  2:12672/1‑149 (MQ=255)
                                                                                                                              tCACACTGTTGCTCGGCTTTGAGGATATTCCTGTTGAGGAAGCGGCTGCCCAGGCGCGAAAGTATCAGAGCGTACAACCGACCGTCGCCAAAGAAGTAAGTCTTAATTGAGGATGAAAATGTCAGTATTTCCAGAAAGTTTTTTATggg                       >  1:3345503/1‑149 (MQ=255)
                                                                                                                              tCACACTGTTGCTCGGCTTTGAGGATATTCCTGTTGAGGAAGCGGCTGCCCAGGCGCGAAAGTATCAGAGAGTACAACCGACCGTCGCAAAAGAAGTAAGTCTTAATTGATGATGAAAATGTCAGTATTTCCAGAAAGTTTTTTATggg                       <  1:3654047/149‑1 (MQ=255)
                                                                                                                                 cacTGTTGCTCGGCTTTGAGGATATTCCTGTTGAGGAAGCGGCTGCCCAGGCGCGAAAGTATCAGAGCGTACAACCGACCGTCGCCAAAGAAGTAAGACTTAATTGAGGATGAAAATGTCAGTATTTCCAGAAAGTTTTTTATGGggct                    >  1:469527/1‑148 (MQ=255)
                                                                                                                                    tGTTGCTCGGCTTTGAGGATATTCCTGTTGAGGAAGCGGCTGCCCAGGCGCGAAAGTATCAGAGCGTACAACCGACCGTCGCCAAAGAAGTAAGTCTTAATTGAGGATGAAAATGTCAGTATTTCCAGAAAGTTTTTTATGGGgcggcg                 >  1:2614697/1‑149 (MQ=255)
                                                                                                                                        gCTCGGCTTTGAGGATATTCCTGTTGAGGAAGCGGCTGCCCAGGCGCGAAAGTATCAGAGCGTACAACCGACCGTCGCCAAAGAAGTAAGTCTTAATTGAGGATGAAAATGTCAGTATTTCCAGAAAGTTTTTTATGGGGCGGCGCGCt             <  1:50580/149‑1 (MQ=255)
                                                                                                                                            ggCTTTGAGGATATTCCTGTTGAGGAAGCGGCTGCCCAGGCGCGAAAGTATCAGAGCGTACAACCGACCGTCGCCAAAGAAGTAAGTCTTAATTGAGGATGAAAATGTCAGTATTTCCAGAAAGTTTTTTATGGGGCGGCGCGCTTgcc         >  1:4964315/1‑149 (MQ=255)
                                                                                                                                             gCTTTGAGGATATTCCTGTTGAGGAAGCGGCTGCCCAGGCGCGAAAGTATCAGAGCGTACAACCGACCGTCGCCAAAGAAGTAAGTCTTAATTGAGGATGAAAATGTCAGTATTTCCAGAAAGTTTTTTATGGGGCGGCGCGCTTgccg        >  1:201398/1‑149 (MQ=255)
                                                                                                                                              cTTTGAGGATATTCCTGTTGAGGAAGCGGCTGCCCAGGCGCGAAAGTATCAGAGCGTACAACCGACCGTCGCCAAAGAAGTAAGTCTTAATTGAGGATGAAAATGTCAGTATTTCCAGAAAGTTTTTTATGGGGCGGCGCGCTTgccgc       >  1:700499/1‑149 (MQ=255)
                                                                                                                                               tttGAGGATATTCCTGTTGAGGAAGCGGCTGCCCAGGCGCGAAAGTATCAGAGCGTACAACCGACCGTCGCCAAAGAAGTAAGTCTTAATTGAGGATGAAAATGTCAGTATTTCCAGAAAGTTTTTTATGGGGCGGCGCGCTTgccgcc      >  1:2872558/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
TTGCCAGCCACTCAATGGCAGCGCCGGGGCTATTTACCAGCGTGCAGTTCTTCGATCCGGCGAATCCAATGAGCATCGTCTGGGTGTTCGCGGTCATGGCGCTGGCGGTGGTGCTGTCGTTTATCCTCACACTGTTGCTCGGCTTTGAGGATATTCCTGTTGAGGAAGCGGCTGCCCAGGCGCGAAAGTATCAGAGCGTACAACCGACCGTCGCCAAAGAAGTAAGTCTTAATTGAGGATGAAAATGTCAGTATTTCCAGAAAGTTTTTTATGGGGCGGCGCGCTTGCCGCCAACC  >  CP009273/2834105‑2834400

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑