Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,876,760 C→T 89.3% G246G (GGG→GGA)  casA ← CRISP RNA (crRNA) containing Cascade antiviral complex protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,876,7600CT89.3% 826.1 / 72.9 271G246G (GGG→GGA) casACRISP RNA (crRNA) containing Cascade antiviral complex protein
Reads supporting (aligned to +/- strand):  ref base C (12/17);  new base T (122/120);  total (134/137)
Fisher's exact test for biased strand distribution p-value = 4.33e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

Only 100 of 273 total aligned reads displayed.

TCCTCAACCTCCCCTTTCTTGACTGTTACCAGACAAGGGGAATGCGGATGGGGCCATAGCCCATTAACTGTAAAGGTAAATTTTTCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATCCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACGGACAAACCCAATTGACGAAGCAGGTATAGACTCATTGGACTTGATAGGTTTAATCCAGGTAGGTTGGTTTTCCGTATGTGATTCATTAGGAAATT  >  CP009273/2876614‑2876905
                                                                                                                                                  |                                                                                                                                                 
  cTCAACCTCCCCTTTCTTGACTGTTACCAGACAAGGGGAATGCGGATGGGGCCATAGCCCATTAACTGTAAAGGTAAATTTTTCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCaa                                                                                                                                               >  1:3600361/1‑149 (MQ=255)
     aaCCTCCCCTTTCTTGACTGTTACCAGACAAGGGGAATGCGGATGGGGCCATAGCCCATTAACTGTAAAGGTAAATTTTTCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATCCCAATgg                                                                                                                                            >  1:3086756/1‑149 (MQ=255)
       ccTCCCCTTTCTTGACTGTTACCAGACAAGGGGAATGCGGATGGGGCCATAGCCCATTAACTGTAAAGGTAAATTTTTCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGa                                                                                                                                          >  1:597651/1‑149 (MQ=255)
        cTCCCCTTTCTTGACTGTTACCAGACAAGGGGAATGCGGATGGGGCCATAGCCCATTAACTGTAAAGGTAAATTTTTCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATCCCAATGGGAt                                                                                                                                         <  2:4565707/149‑1 (MQ=255)
        cTCCCCTTTCTTGACTGTTACCAGACAAGGGGAATGCGGATGGGGCCATAGCCCATTAACTGGAAAGGTAAATTTTTCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGAt                                                                                                                                         <  1:4596713/149‑1 (MQ=255)
         tCCCCTTTCTTGACTGTTACCAGACAAGGGGAATGCGGATGGGGCCATAGCCCATTAACTGTAAAGGTAAATTTTTCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATc                                                                                                                                        <  2:4027250/149‑1 (MQ=255)
         tCCCCTTTCTTGACTGTTACCAGACAAGGGGAATGCGGATGGGGCCATAGCCCATTAACTGTAAAGGTAAATTTTTCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATc                                                                                                                                        >  1:3467392/1‑149 (MQ=255)
             cTTTCTTGACTGTTACCAGACAAGGGGAATGCGGATGGGGCCATAGCCCATTAACTGTAAAGGTAAATTTTTCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCAt                                                                                                                                    <  2:72912/149‑1 (MQ=255)
               ttCTTGACTGTTACCAGACAAGGGGAATGCGGATGGGGCCATAGCCCATTAACTGTAAAGGTAAATTTTTCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATCCCAATGGGATCGCATaa                                                                                                                                  <  2:3681155/149‑1 (MQ=255)
                tCTTGACTGTTACCAGACAAGGGGAATGCGGATGGGGCCATAGCCCATTAACTGTAAAGGTAAATTTTTCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAAt                                                                                                                                 >  2:1178870/1‑149 (MQ=255)
                    gACTGTTACCAGACAAGGGGAATGCGGATGGGGCCATAGCCCATTAACTGTAAAGGTAAATTTTTCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCaa                                                                                                                             <  2:1915902/149‑1 (MQ=255)
                       tGTTACCAGACAAGGGGAATGCGGATGGGGCCATAGCCCATTAACTGTAAAGGTAAATTTTTCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAatat                                                                                                                          <  2:582694/149‑1 (MQ=255)
                       tGTTACCAGACAAGGGGAATGCGGATGGGGCCATAGCCCATTAACTGTAAAGGTAAATTTTTCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAatat                                                                                                                          <  2:3289458/149‑1 (MQ=255)
                       tGTTACCAGACAAGGGGAATGCGGATGGGGCCATAGCCCATTAACTGTAAAGGTAAATTTTTCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAatat                                                                                                                          <  1:1122809/149‑1 (MQ=255)
                        gTTACCAGACAAGGGGAATGCGGATGGGGCCATAGCCCATTAACTGTAAAGGTAAATTTTTCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATg                                                                                                                         >  1:2428575/1‑149 (MQ=255)
                        gTTACCAGACAAGGGGAATGCGGATGGGGCCATAGCCCATTAACTGTAAAGGTAAATTTTTCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATg                                                                                                                         <  2:2432698/149‑1 (MQ=255)
                         ttACCAGACAAGGGGAATGCGGATGGGGCCATAGCCCATTAACTGTAAAGGTAAATTTTTCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATgc                                                                                                                        >  1:3507933/1‑149 (MQ=255)
                         ttACCAGACAAGGGGAATGCGGATGGGGCCATAGCCCATTAACTGTAAAGGTAAATTTTTCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATgc                                                                                                                        <  2:1844138/149‑1 (MQ=255)
                           aCCAGACAAGGGGAATGCGGATGGGGCCATAGCCCATTAACTGTAAAGGTAAATTTTTCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATCCCAATGGGATCGCATAATTCAATATgcgc                                                                                                                      <  1:1720401/149‑1 (MQ=255)
                              aGACAAGGGGAATGCGGATGGGGCCATAGCCCATTAACTGTAAAGGTAAATTTTTCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTgg                                                                                                                   <  2:380965/149‑1 (MQ=255)
                               gACAAGGGGAATGCGGATGGGGCCATAGCCCATTAACTGTAAAGGTAAATTTTTCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGt                                                                                                                  >  1:3693942/1‑149 (MQ=255)
                               gACAAGGGGAATGCGGATGGGGCCATAGCCCATTAACTGTAAAGGTAAATTTTTCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGt                                                                                                                  >  2:2708150/1‑149 (MQ=255)
                                aCAAGGGGAATGCGGATGGGGCCATAGCCCATTAACTGTAAAGGTAAATTTTTCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGtt                                                                                                                 <  1:4306892/149‑1 (MQ=255)
                                 cAAGGGGAATGCGGATGGGGCCATAGCCCATTAACTGTAAAGGTAAATTTTTCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTg                                                                                                                >  1:2754944/1‑149 (MQ=255)
                                  aaGGGGAATGCGGATGGGGCCATAGCCCATTAACTGTAAAGGTAAATTTTTCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGc                                                                                                               <  1:1327203/149‑1 (MQ=255)
                                  aaGGGGAATGCGGATGGGGCCATAGCACATTAACTGTAAAGGTAAATTTTTCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGc                                                                                                               <  1:1791465/149‑1 (MQ=255)
                                   aGGGGAATGCGGATGGGGCCATAGCCCATTAACTGTAAAGGTAAATTTTTCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGcc                                                                                                              >  1:1965387/1‑149 (MQ=255)
                                   aGGGGAATGCGGATGGGGCCATAGCCCATTAACTGTAAAGGTAAATTTTTCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATCCCAATGGGATCGCATAATTCAATATGCGCTGGTTGcc                                                                                                              >  2:371469/1‑149 (MQ=255)
                                    ggggAATGCGGATGGGGCCATAGCCCATTAACTGTAAAGGTAAATTTTTCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCa                                                                                                             >  1:1551234/1‑149 (MQ=255)
                                     gggAATGCGGATGGGGCCAGAGCCCATTAACTGTAAAGGTAAATTTTTCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATCCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAg                                                                                                            <  2:4603225/149‑1 (MQ=255)
                                         aTGCGGATGGGGCCATAGCCCATTAACTGTAAAGGTAAATTTTTCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATa                                                                                                        <  2:1069475/149‑1 (MQ=255)
                                          tGCGGATGGGGCCATAGCCCATTAACTGTAAAGGTAAATTTTTCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAg                                                                                                       <  1:4418048/149‑1 (MQ=255)
                                              gATGGGGCCATAGCCCATTAACTGTAAAGGTAAATTTTTCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCa                                                                                                   >  1:1432007/1‑149 (MQ=255)
                                                tGGGGCCATAGCCCATTAACTGTAAAGGTAAATTTTTCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACg                                                                                                 <  1:3704222/149‑1 (MQ=255)
                                                tGGGGCCATAGCCCATTAACTGTAAAGGTAAATTTTTCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACg                                                                                                 <  1:690021/149‑1 (MQ=255)
                                                  gggCCATAGCCCATTAACTGTAAAGGTAAATTTGTCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACGGa                                                                                               <  2:5016266/149‑1 (MQ=255)
                                                   ggCCATAGCCCATTAACTGTAAAGGTAAATTTTTCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACGGAc                                                                                              <  1:375851/149‑1 (MQ=255)
                                                      cATAGCCCATTAACTGTAAAGGTAAATTTTTCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACGGACaaa                                                                                           <  1:2876444/149‑1 (MQ=255)
                                                        tAGCCCATTAACTGTAAAGGTAAATTTTTCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACGGACAAAcc                                                                                         >  2:4134613/1‑149 (MQ=255)
                                                         aGCCCATTAACTGTAAAGGTAAATTTTTCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACGGACAAAccc                                                                                        <  1:3833962/149‑1 (MQ=255)
                                                         aGCCCATTAACTGTAAAGGTAAATTTTTCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACGGACAAAccc                                                                                        <  1:4962470/149‑1 (MQ=255)
                                                           cccATTAACTGTAAAGGTAAATTTTTCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACCGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACGGACAAACCCaa                                                                                      <  2:2979466/149‑1 (MQ=255)
                                                            ccATTAACTGTAAAGGTAAATTTTTCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACGGACAAACCCAAt                                                                                     <  2:4271602/149‑1 (MQ=255)
                                                            ccATTAACTGTAAAGGTAAATTTTTCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACGGACAAACCCAAt                                                                                     <  1:5200058/149‑1 (MQ=255)
                                                             cATTAACTGTAAAGGTAAATTTTTCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACGGACAAACCCAAtt                                                                                    <  1:1777240/149‑1 (MQ=255)
                                                              aTTAACTGTAAAGGTAAATTTTTCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACGGACAAACCCAATTg                                                                                   >  2:123018/1‑149 (MQ=255)
                                                               ttAACTGTAAAGGTAAATTTTTCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACGGACAAACCCAATTGa                                                                                  <  2:2147767/149‑1 (MQ=255)
                                                                    tGTAAAGGTAAATTTTTCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACGGACAAACCCAATTGACGAAg                                                                             >  1:4684950/1‑149 (MQ=255)
                                                                    tGTAAAGGTAAATTTTTCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACGGACAAACCCAATTGACGAAg                                                                             >  2:529304/1‑149 (MQ=255)
                                                                    tGTAAAGGTAAATTTTTCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACGGACAAACCCAATTGACGAAg                                                                             >  2:2085595/1‑149 (MQ=255)
                                                                    tGTAAAGGTAAATTTTTCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATCCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACGGACAAACCCAATTGACGAAg                                                                             >  1:1880996/1‑149 (MQ=255)
                                                                      tAAAGGTAAATTTTTCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATCCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACGGACAAACCCAATTGACGAAGCa                                                                           >  2:2743202/1‑149 (MQ=255)
                                                                      tAAAGGTAAATTTTTCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATCCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACGGACAAACCCAATTGACGAAGCa                                                                           <  2:2664313/149‑1 (MQ=255)
                                                                       aaaGGTAAATTTTTCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACGGACAAACCCAATTGACGAAGCAg                                                                          >  2:472721/1‑149 (MQ=255)
                                                                       aaaGGTAAATTTTTCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACGGACAAACCCAATTGACGAAGCAg                                                                          >  2:2269204/1‑149 (MQ=255)
                                                                        aaGGTAAATTTTTCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACGGACAAACCCAATTGACGAAGCAgg                                                                         >  2:312646/1‑149 (MQ=255)
                                                                         aGGTAAATTTTTCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACGGACAAACCCAATTGACGAAGCAGGt                                                                        >  2:3147616/1‑149 (MQ=255)
                                                                              aaTTTTTCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACGGACAAACCCAATTGACGAAGCAGGTATAGa                                                                   <  2:531037/149‑1 (MQ=255)
                                                                              aaTTTTTCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACGGACAAACCCAATTGACGAAGCAGGTATAGa                                                                   >  2:1928071/1‑149 (MQ=255)
                                                                               aTTTTTCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACGGACAAACCCAATTGACGAAGCAGGTATAGAc                                                                  >  1:3294580/1‑149 (MQ=255)
                                                                                tttttCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACGGACAAACCCAATTGACGAAGCAGGTATAGACt                                                                 <  2:2529786/149‑1 (MQ=255)
                                                                                  tttCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACGGACAAACCCAATTGACGAAGCAGGTATAGACTCa                                                               <  1:4509994/149‑1 (MQ=255)
                                                                                    tCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACGGACAAACCCAATTGACGAAGCAGGTATAGACTCAtt                                                             <  1:2711030/149‑1 (MQ=255)
                                                                                       ttAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACGGACAAACCCAATTGACGAAGCAGGTATAGACTCATTGGa                                                          <  2:4164538/149‑1 (MQ=255)
                                                                                        tAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACGGACAAACCCAATTGACGAAGCAGGTATAGACTCATTGGAc                                                         <  2:2823379/149‑1 (MQ=255)
                                                                                         aaGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACGGACAAACCCAATTGACGAAGCAGGTATAGACTCATTGGACt                                                        >  1:3295297/1‑149 (MQ=255)
                                                                                           gAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACGGACAAACCCAATTGACGAAGCAGGTATAGACTCATTGGACTTg                                                      >  2:505678/1‑149 (MQ=255)
                                                                                                   gTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACGGACAAACCCAATTGACGAAGCAGGTATAGACTCATTGGACTTGATAGGttt                                              >  1:72844/1‑149 (MQ=255)
                                                                                                     ataACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACGGACAAACCCAATTGACGAAGCAGGTATAGACTCATTGGACTTGATAGGTTTaa                                            >  2:5112533/1‑149 (MQ=255)
                                                                                                     ataACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACGGACAAACCCAATTGACGAAGCAGGTATAGACTCATTGGACTTGATAGGTTTaa                                            >  2:1552796/1‑149 (MQ=255)
                                                                                                     ataACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACGGACAAACCCAATTGACGAAGCAGGTATAGACTCATTGGACTTGATAGGTTTaa                                            >  1:1050686/1‑149 (MQ=255)
                                                                                                       aaCGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACGGACAAACCCAATTGACGAAGCAGGTATAGACTCATTGGACTTGATAGGTTTAATc                                          >  1:1616578/1‑149 (MQ=255)
                                                                                                         cGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACGGACAAACCCAATTGACGAAGCAGGTATAGACTCATTGGACTTGATAGGTTTAATCCa                                        <  1:3367806/149‑1 (MQ=255)
                                                                                                         cGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACGGACAAACCCAATTGACGAAGCAGGTATAGACTCATTGGACTTGATAGGTTTAATCCa                                        <  1:2603461/149‑1 (MQ=255)
                                                                                                            aaaTTGCTTTCCTGTCCACAGCAAGAACATTTACCAATCCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACGGACAAACCCAATTGACGAAGCAGGTATAGACTCATTGGACTTGATAGGTTTAATCCaggt                                     >  1:3403743/1‑149 (MQ=255)
                                                                                                              aTTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACGGACAAACCCAATTGACGAAGCAGGTATAGACTCATTGGACTTGATAGGTTTAATCCaggtag                                   <  2:1312685/149‑1 (MQ=255)
                                                                                                              aTTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACGGACAAACCCAATTGACGAAGCAGGTATAGACTCATTGGACTTGATAGGTTTAATCCaggtag                                   >  1:4108154/1‑149 (MQ=255)
                                                                                                                     tCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACGGACAAACCCAATTGACGAAGCAGGTATAGACTCATTGGACTTGATAGGTTTAATCCAGGTAggttggtt                            >  1:419239/1‑149 (MQ=255)
                                                                                                                      ccTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACGGACAAACCCAATTGACGAAGCAGGTATAGACTCATTGGACTTGATAGGTTTAATCCAGGTAGGTTGGttt                           <  2:3149078/149‑1 (MQ=255)
                                                                                                                      ccTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACGGACAAACCCAATTGACGAAGCAGGTATAGACTCATTGGACTTGATAGGTTTAATCCAGGTAGGTTGGttt                           >  1:511117/1‑149 (MQ=255)
                                                                                                                      ccTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACGGACAAACCCAATTGACGAAGCAGGTATAGACTCATTGGACTTGATAGGTTTAATCCAGGTAGGTTGGttt                           <  1:2059632/149‑1 (MQ=255)
                                                                                                                       cTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACGGACAAACCCAATTGACGAAGCAGGTATAGACTCATTGGACTTGATAGGTTTAATCCAGGTAGGTTGGtttt                          <  1:5041221/149‑1 (MQ=255)
                                                                                                                       cTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACGGACAAACCCAATTGACGAAGCAGGTATAGACTCATTGGACTTGATAGGTTTAATCCAGGTAGGTTGGtttt                          >  2:3631777/1‑149 (MQ=255)
                                                                                                                       cTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACGGACAAACCCAATTGACGAAGCAGGTATAGACTCATTGGACTTGATAGGTTTAATCCAGGTAGGTTGGtttt                          <  1:5040527/149‑1 (MQ=255)
                                                                                                                              caGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACGGACAAACCCAATTGACGAAGCAGGTATAGACTCATTGGACTTGATAGGTTTAATCCAGGTAGGTTGGTTTTCCGTAtg                   >  1:488320/1‑149 (MQ=255)
                                                                                                                              caGCAAGAACATTTACCAATCCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACGGACAAACCCAATTGACGAAGCAGGTATAGACTCATTGGACTTGATAGGTTTAATCCAGGTAGGTTGGTTTTCCGTAtg                   >  1:797594/1‑149 (MQ=255)
                                                                                                                               aGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACGGACAAACCCAATTGACGAAGCAGGTATAGACTCATTGGACTTGATAGGTTTAATCCAGGTAGGTTGGTTTTCCGTAtgt                  <  2:4919595/149‑1 (MQ=255)
                                                                                                                                gCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACGGACAAACCCAATTGACGAAGCAGGTATAGACTCATTGGACTTGATAGGTTTAATCCAGGTAGGTTGGTTTTCCGTAtgtg                 >  2:3009127/1‑149 (MQ=255)
                                                                                                                                gCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACGGACAAACCCAATTGACGAAGCAGGTATAGACTCATTGGACTTGATAGGTTTAATCCAGGTAGGTTGGTTTTCCGTAtgtg                 >  2:2364398/1‑149 (MQ=255)
                                                                                                                                   aGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACGGACAAACCCAATTGACGAAGCAGGTATAGACTCATTGGACTTGATAGGTTTAATCCAGGTAGGTTGGTTTTCCGTATGTGAtt              <  2:3693329/149‑1 (MQ=255)
                                                                                                                                   aGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACGGACAAACCCAATTGACGAAGCAGGTATAGACTCATTGGACTTGATAGGTTTAATCCAGGTAGGTTGGTTTTCCGTATGTGAtt              >  1:752085/1‑149 (MQ=255)
                                                                                                                                    gAACATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACGGACAAACCCAATTGACGAAGCAGGTATAGACTCATTGGACTTGATAGGTTTAATCCAGGTAGGTTGGTTTTCCGTATGTGATTc             >  2:1413093/1‑149 (MQ=255)
                                                                                                                                      aCATTTACCAATCCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACGGACAAACCCAATTGACGAAGCAGGTATAGACTCATTGGACTTGATAGGTTTAATCCAGGTAGGTTGGTTTTCCGTATGTGATTCAt           <  2:818851/149‑1 (MQ=255)
                                                                                                                                       cATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACGGACAAACCCAATTGACGAAGCAGGTATAGACTCATTGGACTTGATAGGTTTAATCCAGGTAGGTTGGTTTTCCGTATGTGATTCAtt          >  2:4865415/1‑149 (MQ=255)
                                                                                                                                       cATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACGGACAAACCCAATTGACGAAGCAGGTATAGACTCATTGGACTTGATAGGTTTAATCCAGGTAGGTTGGTTTTCCGTATGTGATTCAtt          >  1:4906919/1‑149 (MQ=255)
                                                                                                                                        aTTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACGGACAAACCCAATTGACGAAGCAGGTATAGACTCATTGGACTTGATAGGTTTAATCCAGGTAGGTTGGTTTTCCGTATGTGATTCATTa         >  2:187529/1‑149 (MQ=255)
                                                                                                                                          ttACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACGGACAAACCCAATTGACGAAGCAGGTATAGACTCATTGGACTTGATAGGTTTAATCCAGGTAGGTTGGTTTTCCGTATGTGATTCATTAgg       <  2:4178302/149‑1 (MQ=255)
                                                                                                                                          ttACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACGGACAAACCCAATTGACGAAGCAGGTATAGACTCATTGGACTTGATAGGTTTAATCCAGGTAGGTTGGTTTTCCGTATGTGATTCATTAgg       <  2:3313491/149‑1 (MQ=255)
                                                                                                                                            aCCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACGGACAAACCCAATTGACGAAGCAGGTATAGACTCATTGGACTTGATAGGTTTAATCCAGGTAGGTTGGTTTTCCGTATGTGATTCATTAGGaa     >  2:2896164/1‑149 (MQ=255)
                                                                                                                                               aaTTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACGGACAAACCCAATTGACGAAGCAGGTATAGACTCATTGGACTTGATAGGTTTAATCCAGGTAGGTTGGTTTTCCGTATGTGATTCATTAGGAAAtt  <  2:4247/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
TCCTCAACCTCCCCTTTCTTGACTGTTACCAGACAAGGGGAATGCGGATGGGGCCATAGCCCATTAACTGTAAAGGTAAATTTTTCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATCCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACGGACAAACCCAATTGACGAAGCAGGTATAGACTCATTGGACTTGATAGGTTTAATCCAGGTAGGTTGGTTTTCCGTATGTGATTCATTAGGAAATT  >  CP009273/2876614‑2876905

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑