BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	2,899,859	G→T	6.2%	G264C (GGT→TGT)	ygcG →	uncharacterized protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	2,899,859	0	G	T	6.2%	1297.1 / 45.0	340	G264C (GGT→TGT)	ygcG	uncharacterized protein
Reads supporting (aligned to +/- strand): ref base G (179/140); new base T (13/8); total (192/148)
Fisher's exact test for biased strand distribution p-value = 6.56e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.29e-01

Only 100 of 344 total aligned reads displayed.

TCTTTTAGATTTATTCCTGTTCTCCGATAAAATTTTTTCCATTGCTGTATTTTCCTTCTTTTTTACTTTCACCATATTTATGGTCTTTACCTGTTTATGTGTACTTCAGAAAAGAGCATCTGGTAGAAGTTATCATTCAGACAACAGCGGTTCCGCTGGAGGCTCAGATAGCGGCGGATTTAGCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGC  >  CP009273/2899711‑2900007

   tttAGATTTATTCCTGTTCTCCGATAAAAATTTTAACATTGATGTATTTTCCTTCTTTTATACTTTCACCATATTTATGGTCTATACCTGTTTATCTGTACTTCAGAAAAGAGCATCTGGAAGAAGTTATCAATAAGACAACAGCGGtt                                                                                                                                                   >  1:1079541/1‑149 (MQ=255)

     tAGATTTATTCCTGTTCTCCGATAAAATTTTTTCCATTGCTGTATTTTCCTTCTTTTTTACTTTCACCATATTTATGGTCTTTACCTGTTTATGTGTACTTCAGAAAAGAGCATCTGGTAGAAGTTATCATTCAGACAACAGCGGTTcc                                                                                                                                                 >  2:1057417/1‑149 (MQ=255)

     tAGATTTATTCCTGTTCTCCGATAAAATTTTTTCCATTGCTGTATTTTCCTTCTTTTTTACTTTCACCATATTTATGGTCTTTACCTGTTTATGTGTACTTCAGAAAAGAGCATCTGGTAGAAGTTATCATTCAGACAACAGCGGTTcc                                                                                                                                                 <  1:2342621/149‑1 (MQ=255)

        aTTTATTCCTGTTCTCCGATAAAATTTTTTCCATTGCTGTATTTTCCTTCTTTTTTACTTTCACCATATTTATGGTCTTTACCTGTTTATGTGTACTTCAGAAAAGAGCATCTGGTAGAAGTTATCATTCAGACAACAGCGGTTCCGCt                                                                                                                                              >  2:4826998/1‑149 (MQ=255)

         tttATTCCTGTTCTCCGATAAAATTTTTTCCATTGCTGTATTTTCCTTCTTTTTTACTTTCACCATATTTATGGTCTTTACCTGTTTATGTGTACTTCAGAAAAGAGCATCTGGTAGAAGTTATCATTCAGACAACAGCGGTTCCGCTg                                                                                                                                             >  2:4062995/1‑149 (MQ=255)

              tCCTGTTCTCCGATAAAATTTTTTCCATTGCTGTATTTTCCTTCTTTTTTACTTTCACCATATTTATGGTCTTTACCTGTTTATGTGTACTTCAGAAAAGAGCATCTGGTAGAAGTTATCATTCAGACAACAGCGGTTCCGCTGGAGGc                                                                                                                                        >  1:1249720/1‑149 (MQ=255)

               ccTGTTCTCCGATAAAATTTTTTCCATTGCTGTATTTTCCTTCTTTTTTACTTTCACCATATTTATGGTCTTTACCTGTTTATGTGTACTTCAGAAAAGAGCATCTGGTAGAAGTTATCATTCAGACAACAGCGGTTCCGCTGGAGGCt                                                                                                                                       >  2:640880/1‑149 (MQ=255)

                  gTTCTCCGATAAAATTTTTTCCATTGCTGTATTTTCCTTCTTTTTTACTTTCACCATATTTATGGTCTTTACCTGTTTATGTGTACTTCAGAAAAGAGCATCTGGTAGAAGTTATCATTCAGACAACAGCGGTTCCGCTGGAGGCTCAg                                                                                                                                    >  2:232169/1‑149 (MQ=255)

                   ttCTCCGATAAAATTTTTTCCATTGCTGTATTTTCCTTCTTTTTTACTTTCACCATATTTATGGTCTTTACCTGTTTATGTGTACTTCAGAAAAGAGCATCTGGTAGAAGTTATCATTCAGACAACAGCGGTTCCGCTGGAGGCTCAGa                                                                                                                                   <  1:142943/149‑1 (MQ=255)

                       ccGATAAAATTTTTTCCATAGCTGTATTTTCCTTCTTTTTTACTTTCACCATATTTATGGTCTTTACCTTTTTATGTGTACATCAGAAAATAGCATCTGGTAGAAGTTATCATTCAGACAACAGCGGTTACGCTGGAGGCTCAGATAgc                                                                                                                               >  1:1974494/1‑149 (MQ=255)

                          aTAAAATTTTTTCCATTGCTGTATTTTCCTTCTTTTTTACTTTCACCATATTTATGGTCTTTACCTGTTTATGTGTACTTCAGAAAAGAGCATCTGGTAGAAGTTATCATTCAGACAACAGCGGTTCCGCTGGAGGCTCAGATAgcggc                                                                                                                            >  2:4417273/1‑149 (MQ=255)

                          aTAAAATTTTTTCCATTGCTGTATTTTCCTTCTTTTTTACTTTCACCATATTTATGGTCTTTACCTGTTTATGTGTACTTCAGAAAAGAGCATCTGGTAGAAGTTATCATTCAGACAACAGCGGTTCCGCTGGAGGCTCAGATAgcggc                                                                                                                            >  2:2510303/1‑149 (MQ=255)

                           tAAAATTTTTTACATTGCTGTATTTTACTTCTTTTTTACTTTAAAAATATTTATGGTCTTTACATGTTTATGTTTACTTCATAAAATAGCATCTGGTATAAGTTATCATTAAGACAACAGCGGTTCCGCTGTAGGCTAAGATAgcggcg                                                                                                                           >  1:5247325/1‑149 (MQ=255)

                            aaaaTTTTTTCCATTGCTGTATTTTCCTTCTTTTTTACTTTCACCATATTTATGGTCTTTACCTGTTTATGTGTACTTCAGAAAAGAGCATCTGGTAGAAGTTATCATTCAGACAACAGCGGTTCCGCTGGAGGCTCAGATAGcggcgg                                                                                                                          >  2:4115573/1‑149 (MQ=255)

                               aTTTTTTCCATTGCTGTATTTTCCTTCTTTTTTACTTTCACCATATTTATGGTCTTTACCTGTTTATGTGTACTTCAGAAAAGAGCATCTGGTAGAAGTTATCATTCAGACAACAGCGGTTCCGCTGGAGGCTCAGATAGCGGCGGAtt                                                                                                                       <  1:3899539/149‑1 (MQ=255)

                                   tttCCATTGCTGTATTTTCCTTCTTTTTTACTTTCACCATATTTATGGTCTTTACCTGTTTATGTGTACTTCAGAAAAGAGCATCTGGTAGAAGTTATCATTCAGACAACAGCGGTTCCGCTGGAGGCTCAGATAGCGGCGGATTTAgc                                                                                                                   <  2:4538665/149‑1 (MQ=255)

                                     tCCATTGCTGTATTTTCCTTCTTTTTTACTTTCACCATATTTATGGTCTTTACCTGTTTATGTGTACTTCAGAAAAGAGCATCTGGTAGAAGTTATCATTCAGACAACAGCGGTTCCGCTGGAGGCTCAGATAGCGGCGGATTTAgcgg                                                                                                                 >  1:911549/1‑149 (MQ=255)

                                        aTTGCTGTATTTTCCTTCTTTTTTACTTTCACCATATTTATGGTCTTTACCTGTTTATGTGTACTTCAGAAAAGAGCATCTGGTAGAAGTTATCATTCAGACAACAGCGGTTCCGCTGGAGGCTCAGATAGCGGCGGATTTAGcggcgg                                                                                                              >  2:1105723/1‑149 (MQ=255)

                                           gCTGTATTTTCCTTCTTTTTTACTTTCACCATATTTATGGTCTTTACCTGTTTATGTGTACTTCAGAAAAGAGCATCTGGTAGAAGTTATCATTCAGACAACAGCGGTTCCGCTGGAGGCTCAGATAGCGGCGGATTTAGCGGCggggg                                                                                                           <  2:17093/149‑1 (MQ=255)

                                           gCTGTATTTTCCTTCTTTTTTACTTTCACCATATTTATGGTCTTTACCTGTTTATGTGTACTTCAGAAAAGAGCATCTGGTAGAAGTTATCATTCAGACAACAGCGGTTCCGCTGGAGGCTCAGATAGCGGCGGATTTAGCGGCggggg                                                                                                           <  1:3228859/149‑1 (MQ=255)

                                            cTGTATTTTCCTTCTTTTTTACTTTCACCATATTTATGGTCTTTACCTGTTTATGTGTACTTCAGAAAAGAGCATCTGGTAGAAGTTATCATTCAGACAACAGCGGTTCCGCTGGAGGCTCAGATAGCGGCGGATTTAGCGGCGGGGGc                                                                                                          >  1:1307157/1‑149 (MQ=255)

                                             tGTATTTTCCTTCTTTTTTACTTTCACCATATTTATGGTCTTTACCTGTTTATGTGTACTTCAGAATAGAGCATCTGGTAGAAGTTATCATTCAGACAACAGCGGTTCCGCTGGAGGCTCAGATAGCGGCGGATTTAGCGGCGGGGGCg                                                                                                         >  2:1317744/1‑149 (MQ=255)

                                              gTATTTTCCTTCTTTTTTACTTTCACCATATTTATGGTCTTTACCTGTTTATGTGTACTTCAGAAAAGAGCATCTGGTAGAAGTTATCATTCAGACAACAGCGGTTCCGCTGGAGGCTCAGATAGCGGCGGATTTAGCGGCGGGGGCgg                                                                                                        >  2:1558372/1‑149 (MQ=255)

                                              gTATTTTCCTTCTTTTTTACTTTCACCATATTTATGGTCTTTACCTGTTTATGTGTACTTCAGAAAAGAGCATCTGGTAGAAGTTATCATTCAGACAACAGCGGTTCCGCTGGAGGCTCAGATAGCGGCGGATTTAGCGGCGGGGGCgg                                                                                                        >  2:326129/1‑149 (MQ=255)

                                                  tttCCTTCTTTTTTACTTTCACCATATTTATGGTCTTTACCTGTTTATGTGTACTTCAGAAAAGAGCATCTGGTAGAAGTTATCATTCAGACAACAGCGGTTCCGCTGGAGGCTCAGATAGCGGCGGATTTAGCGGCGGGGGCGGttct                                                                                                    >  1:4890948/1‑149 (MQ=255)

                                                  tttCCTTCTTTTTTACTTTCACCATATTTATGGTCTTTACCTGTTTATGTGTACTTCAGAAAAGAGCATCTGGTAGAAGTTATCATTCAGACAACAGCGGTTCCGCTGGAGGCTCAGATAGCGGCGGATTTAGCGGCGGGGGCGGttct                                                                                                    >  1:3529412/1‑149 (MQ=255)

                                                  tttCCTTCTTTTTTACTTTCACCATATTTATGGTCTTTACCTGTTTATGTGTACTTCAGAAAAGAGCATCTGGTAGAAGTTATCATTCAGACAACAGCGGTTCCGCTGGAGGCTCAGATAGCGGCGGATTTAGCGGCGGGGGCGGttct                                                                                                    >  1:875306/1‑149 (MQ=255)

                                                    tCCTTCTTTTTTACTTTCACCATATTTATGGTCTTTACCTGTTTATGTGTACTTCAGAAAAGAGCATCTGGTAGAAGTTATCATTCAGACAACAGCGGTTCCGCTGGAGGCTCAGATAGCGGCGGATTTAGCGGCGGGGGCGGttcttc                                                                                                  >  1:4745959/1‑149 (MQ=255)

                                                    tCCTTCTTTTTTACTTTCACCATATTTATGGTCTTTACCTGTTTATGTGTACTTCAGAAAAGAGCATCTGGTAGAAGTTATCATTCAGACAACAGCGGTTCCGCTGGAGGCTCAGATAGCGGCGGATTTAGCGGCGGGGGCGGttcttc                                                                                                  >  2:5028354/1‑149 (MQ=255)

                                                      cttcttTTTTACTTTCACCATATTTATGGTCTTTACCTGTTTATGTGTACTTCAGAAAAGAGCATCTGGTAGAAGTTATCATTCAGACAACAGCGGTTCCGCTGGAGGCTCAGATAGCGGCGGATTTAGCGGCGGGGGCGGTTCTTCTg                                                                                                >  2:4453946/1‑149 (MQ=255)

                                                      cttcttTTTTACTTTCACCATATTTATGGTCTTTACCTGTTTATGTGTACTTCAGAAAAGAGCATCTGGTAGAAGTTATCATTCAGACAACAGCGGTTCCGCTGGAGGCTCAGATAGCGGCGGATTTAGCGGCGGGGGCGGTTCTTCTg                                                                                                >  2:2782404/1‑149 (MQ=255)

                                                                  tttCACCATATTTATGGTCTTTACCTGTTTATGTGTACTTCAGAAAAGAGCATCTGGTAGAAGTTATCATTCAGACAACAGCGGTTCCGCTGGAGGCTCAGATAGCGGCGGATTTAGCGGCGGGGGCGGTTCTTCTGGAGGTGgcggcg                                                                                    >  1:3775217/1‑149 (MQ=255)

                                                                   ttCACCATATTTATGGTCTTTACCTGTTTATGTGTACTTCAGAAAAGAGCATCTGGTAGAAGTTATCATTCAGACAACAGCGGTTCCGCTGGAGGCTCAGATAGCGGCGGATTTAGCGGCGGGGGCGGTTCTTCTGGAGGTGGCGgcgc                                                                                   <  1:4456946/149‑1 (MQ=255)

                                                                    tCACCATATTTATGGTCTTTACCTGTTTATGTGTACTTCAGAAAAGAGCATCTGGTAGAAGTTATCATTCAGACAACAGCGGTTCCGCTGGAGGCTCAGATAGCGGCGGATTTAGCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCa                                                                                  >  1:3639468/1‑149 (MQ=255)

                                                                    tCACCATATTTATGGTCTTTACCTGTTTATGTGTACTTCAGAAAAGAGCATCTGGTAGAAGTTATCATTCAGACAACAGCGGTTCCGCTGGAGGCTCAGATAGCGGCGGATTTAGCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCa                                                                                  >  2:157186/1‑149 (MQ=255)

                                                                      aCCATATTTATGGTCTTTACCTGTTTATGTGTACTTCAGAAAAGAGCATCTGGTAGAAGTTATCATTCAGACAACAGCGGTTCCGCTGGAGGCTCAGATAGCGGCGGATTTAGCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATc                                                                                >  1:2563243/1‑149 (MQ=255)

                                                                       ccATATTTATGGTCTTTACCTGTTTATGTGTACTTCAGAAAAGAGCATCTGGTAGAAGTTATCATTCAGACAACAGCGGTTCCGCTGGAGGCTCAGATAGCGGCGGATTTAGCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATcc                                                                               >  1:2416555/1‑149 (MQ=255)

                                                                        cATATTTATGGTCTTTACCTGTTTATGTGTACTTCAGAAAAGAGCATCTGGTAGAAGTTATCATTCAGACAACAGCGGTTCCGCTGGAGGCTCAGATAGCGGCGGATTTAGCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCg                                                                              >  2:2821473/1‑149 (MQ=255)

                                                                         atatTTATGGTCTTTACCTGTTTATGTGTACTTCAGAAAAGAGCATCTGGTAGAAGTTATCATTCAGACAACAGCGGTTCCGCTGGAGGCTCAGATAGCGGCGGATTTAGCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCgg                                                                             >  1:2253924/1‑149 (MQ=255)

                                                                          tatTTATGGTCTTTACCTGTTTATGTGTACTTCAGAAAAGAGCATCTGGTAGAAGTTATCATTCAGACAACAGCGGTTCCGCTGGAGGCTCAGATAGCGGCGGATTTAGCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGc                                                                            >  2:872765/1‑149 (MQ=255)

                                                                           atTTATGGTCTTTACCTGTTTATGTGTACTTCAGAAAAGAGCATCTGGTAGAAGTTATCATTCAGACAACAGCGGTTCCGCTGGAGGCTCAGATAGCGGCGGATTTAGCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGcc                                                                           >  2:2854001/1‑149 (MQ=255)

                                                                             ttATGGTCTTTACCTGTTTATGTGTACTTCAGAAAAGAGCATCTGGTAGAAGTTATCATTCAGACAACAGCGGTTCCGCTGGAGGCTCAGATAGCGGCGGATTTAGCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGc                                                                         >  2:2616349/1‑149 (MQ=255)

                                                                               aTGGTCTTTACCTGTTTATGTGTACTTCAGAAAAGAGCATCTGGTAGAAGTTATCATTCAGACAACAGCGGTTCCGCTGGAGGCTCAGATAGCGGCGGATTTAGCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTg                                                                       <  2:1037425/149‑1 (MQ=255)

                                                                               aTGGTCTTAACCTGTTTATGAGTACTTCATAAAAGAGCATCTTGTAGAAGATATCAGTCAGACAACAGCGGTTCCGCTGGAGGCTCAGATAGCTGCGGATTTAGCGGCGGGGTCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTg                                                                       >  2:1745083/1‑149 (MQ=255)

                                                                                tGGTCTTTACCTGTTTATGTGTACTTCAGAAAAGAGCATCTGGTAGAAGTTATCATTCAGACAACAGCTGTTCCGCTGGAGGCTCAGATAGCGGCGGATTTAGCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTgg                                                                      <  2:2195324/149‑1 (MQ=255)

                                                                                 ggTCTTTACCTGTTTATGTGTACTTCAGAAAAGAGCATCTGGTAGAAGTTATCATTCAGACAACAGCGGTTCCGCTGGAGGCTCAGATAGCGGCGGATTTAGCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGt                                                                     <  1:697772/149‑1 (MQ=255)

                                                                                 ggTCTTTACCTGTTTATGTGTACTTCAGAAAAGAGCATCTGGTAGAAGTTATCATTCAGACAACAGCGGTTCCGCTGGAGGCTCAGATAGCGGCGGATTTAGCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGt                                                                     <  2:3529047/149‑1 (MQ=255)

                                                                                   tCTTTACCTGTTTATGTGTACTTCAGAAAAGAGCATCTGGTAGAAGTTATCATTCAGACAACAGCGGTTCCGCTGGAGGCTCAGATAGCGGCGGATTTAGCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTaa                                                                   >  2:4984216/1‑149 (MQ=255)

                                                                                   tCTTTACCTGTTTATGTGTACTTCAGAAAAGAGCATCTGGTAGAAGTTATCATTCAGACAACAGCGGTTCCGCTGGAGGCTCAGATAGCGGCGGATTTAGCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTaa                                                                   <  2:4233071/149‑1 (MQ=255)

                                                                                           tGTTTATGTGTACTTCAGAAAAGAGCATCTGGTAGAAGTTATCATTCAGACAACAGCGGTTCCGCTGGAGGCTCAGATAGCGGCGGATTTAGCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCa                                                           <  1:4826088/149‑1 (MQ=255)

                                                                                           tGTTTATGTGTACTTCAGAAAAGAGCATCTGGTAGAAGTTATCATTCAGACAACAGCGGTTCCGCTGGAGGCTCAGATAGCGGCGGATTTAGCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCa                                                           >  1:1579870/1‑149 (MQ=255)

                                                                                            gTTTATGTGTACTTCAGAAAAGAGCATCTGGTAGAAGTTATCATTCAGACAACAGCGGTTCCGCTGGAGGCTCAGATAGCGGCGGATTTAGCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCAt                                                          <  1:3454726/149‑1 (MQ=255)

                                                                                            gTTTATGTGTACTTCAGAAAAGAGCATCTGGTAGAAGTTATCATTCAGACAACAGCGGTTCCGCTGGAGGCTCAGATAGCGGCGGATTTAGCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCAt                                                          >  2:1542835/1‑149 (MQ=255)

                                                                                            gTTTATGTGTACTTCAGAAAAGAGCATCTGGTAGAAGTTATCATTCAGACAACAGCGGTTCCGCTGGAGGCTCAGATAGCGGCGGATTTAGCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCAt                                                          <  2:1982118/149‑1 (MQ=255)

                                                                                            gTTTATGTGTACTTCAGAAAAGAGCATCTGGTAGAAGTTATCATTCAGACAACAGCGGTTCCGCTGGAGGCTCAGATAGCGGCGGATTTAGCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCAt                                                          <  1:5021112/149‑1 (MQ=255)

                                                                                             tttATGTGTACTTCAGAAAAGAGCATCTGGTAGAAGTTATCATTCAGACAACAGCGGTTCCGCTGGAGGCTCAGATAGCGGCGGATTTAGCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATa                                                         >  1:2823595/1‑149 (MQ=255)

                                                                                                aTGTGTACTTCAGAAAAGAGCATCTGGTAGAAGTTATCATTCAGACAACAGCGGTTCCGCTGGAGGCTCAGATAGCGGCGGATTTAGCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATaaaa                                                      >  2:437419/1‑149 (MQ=255)

                                                                                                   tgtACTTCAGAAAAGAGCATCTGGTAGAAGTTATCATTCAGACAACAGCTGTTCCGCTGGAGGCTCAGATAGCGGCGGATTTAGCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAAt                                                   >  1:4627009/1‑149 (MQ=255)

                                                                                                       cTTCAGAAAAGAGCATCTGGTAGAAGTTATCATTCAGACAACAGCGGTTCCGCTGGAGGCTCAGATAGCGGCGGATTTAGCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCa                                               <  2:3009886/149‑1 (MQ=255)

                                                                                                       cTTCAGAAAAGAGCATCTGGTAGAAGTTATCATTCAGACAACAGCGGTTCCGCTGGAGGCTCAGATAGCGGCGGATTTAGCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCa                                               <  1:769662/149‑1 (MQ=255)

                                                                                                        ttCAGAAAAGAGCATCTGGTAGAAGTTATCATTCAGACAACAGCGGTTCCGCTGGAGGCTCAGATAGCGGCGGATTTAGCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAg                                              <  2:5207002/149‑1 (MQ=255)

                                                                                                           aGAAAAGAGCATCTGGTAGAAGTTATCATTCAGACAACAGCGGTTCCGCTGGAGGCTCAGATAGCGGCGGATTTAGCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGccc                                           >  1:1613930/1‑149 (MQ=255)

                                                                                                            gAAAAGAGCATCTGGTAGAAGTTATCATTCAGACAACAGCGGTTCCGCTGGAGGCTCAGATAGCGGCGGATTTAGCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCCg                                          >  2:2082652/1‑149 (MQ=255)

                                                                                                              aaaGAGCATCTGGTAGAAGTTATCATTCAGACAACAGCGGTTCCGCTGGAGGCTCAGATAGCGGCGGATTTAGCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTACCTCAACCATAAAAAATGCCAGCCCGGa                                        >  2:4275350/1‑149 (MQ=255)

                                                                                                              aaaGAGCATCTGGTAGAAGTTATCATTCAGACAACAGCGGTTCCGCTGGAGGCTCAGATAGCGGCGGATTTAGCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCCGGa                                        <  1:3867783/149‑1 (MQ=255)

                                                                                                               aaGAGCATCTGGTAGAAGTTATCATTCAGACAACAGCGGTTCCGCTGGAGGCTCAGATAGCGGCGGATTTAGCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCCGGAg                                       <  1:4257815/149‑1 (MQ=255)

                                                                                                                agagCATCTGGTAGAAGTTATCATTCAGACAACAGCGGTTCCGCTGGAGGCTCAGATAGCGGCGGATTTAGCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAAATCAAACATAAAAAATACCAcccccgcgg                                      >  1:3088032/1‑144 (MQ=255)

                                                                                                                  agCATCTGGTAGAAGTTATCATTCAGACAACAGCGGTTCCGCTGGAGGCTCAGATAGCGGCGGATTTAGCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCt                                    <  2:3445186/149‑1 (MQ=255)

                                                                                                                   gCATCTGGTAGAAGTTATCATTCAGACAACAGCGGTTCCGCTGGAGGCTCAGATAGCGGCGGATTTAGCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTg                                   <  2:1972529/149‑1 (MQ=255)

                                                                                                                     aTCTGGTAGAAGTTATCATTCAGACAACAGCGGTTCCGCTGGAGGCTCAGATAGCGGCGGATTTAGCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGGc                                 >  2:1421707/1‑149 (MQ=255)

                                                                                                                     aTCTGGTAGAAGTTATCATTCAGACAACAGCGGTTCCGCTGGAGGCTCAGATAGCGGCGGATTTAGCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGGc                                 >  2:1224582/1‑149 (MQ=255)

                                                                                                                      tCTGGTAGAAGTTATCATTCAGACAACAGCGGTTCCGCTGGAGGCTCAGATAGCGGCGGATTTAGCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCa                                <  2:4745959/149‑1 (MQ=255)

                                                                                                                      tCTGGTAGAAGTTATCAGTCAGACAACAGCGGTTCCGCTGGAGGCTCAGATAGCGGCGGATTTAGCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCa                                <  2:5483/149‑1 (MQ=255)

                                                                                                                       cTGGTAGAAGTTATCATTCAGACAACAGCGGTTCCGCTGGAGGCTCAGATAGCGGCGGATTTAGCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCAt                               <  1:2529186/149‑1 (MQ=255)

                                                                                                                       cTGGTAGAAGTTATCATTCAGACAACAGCGGTTCCGCTGGAGGCTCAGATAGCGGCGGATTTAGCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCAt                               <  2:52274/149‑1 (MQ=255)

                                                                                                                        tGGTAGAAGTTATCATTCAGACAACAGCTGTTCCGCTGGAGGCTCAGATAGCGGCGGATTTAGCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCAtt                              <  1:1204807/149‑1 (MQ=255)

                                                                                                                        tGGTAGAAGTTATCATTCAGACAACAGCGGTTCCGCTGGAGGCTCAGATAGCGGCGGATTTAGCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCAtt                              <  1:2967043/149‑1 (MQ=255)

                                                                                                                         ggTAGAAGTTATCATTCAGACAACAGCGGTTCCGCTGGAGGCTCAGATAGCGGCGGATTTAGCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCAttt                             <  2:2626117/149‑1 (MQ=255)

                                                                                                                          gTAGAAGTTATCATTCAGACAACAGCGGTTCCGCTGGAGGCTCAGATAGCGGCGGATTTAGCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCAtttt                            <  1:3979470/149‑1 (MQ=255)

                                                                                                                           tAGAAGTTATCATTCAGACAACAGCGGTTCCGCTGGAGGCTCAGATAGCGGCGGATTTAGCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCAttttt                           >  2:4552059/1‑149 (MQ=255)

                                                                                                                                gTTATCATTCAGACAACAGCGGTTCCGCTGGAGGCTCAGATAGCGGCGGATTTAGCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCATTTTTAAATc                      >  2:3334826/1‑149 (MQ=255)

                                                                                                                                gTTATCATTCAGACAACAGCGGTTCCGCTGGAGGCTCAGATAGCGGCGGATTTAGCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCATTTTTAAATc                      >  2:3726892/1‑149 (MQ=255)

                                                                                                                                 ttATCATTCAGACAACAGCGGTTCCGCTGGAGGCTCAGATAGCGGCGGATTTAGCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCATTTTTAAATCa                     >  2:1500351/1‑149 (MQ=255)

                                                                                                                                 ttATCATTCAGACAACAGCGGTTCCGCTGGAGGCTCAGATAGCGGCGGATTTAGCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCATTTTTAAATCa                     >  1:3311297/1‑149 (MQ=255)

                                                                                                                                   aTCATTCAGACAACAGCTGTTCCGCTGGAGGCTCAGATAGCGGCGGATTTAGCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCATTTTTAAATCAGa                   <  1:2748390/149‑1 (MQ=255)

                                                                                                                                    tCATTCAGACAACAGCGGTTCCGCTGGAGGCTCAGATAGCGGCGGATTTAGCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCATTTTTAAATCAGAt                  >  1:4318652/1‑149 (MQ=255)

                                                                                                                                    tCATTCAGACAACAGCGGTTCCGCTGGAGGCTCAGATAGCGGCGGATTTAGCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCATTTTTAAATCAGAt                  >  2:1475799/1‑149 (MQ=255)

                                                                                                                                     cATTCAGACAACAGCGGTTCCGCTGGAGGCTCAGATAGCGGCGGATTTAGCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCATTTTTAAATCAGATa                 <  2:911549/149‑1 (MQ=255)

                                                                                                                                     cATTCAGACAACAGCGGTTCCGCTGGAGGCTCAGATAGCGGCGGATTTAGCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCATTTTTAAATCAGATa                 >  1:1509146/1‑149 (MQ=255)

                                                                                                                                      aTTCAGACAACAGCGGTTCCGCTGGAGGCTCAGATAGCGGCGGATTTAGCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCATTTTTAAATCAGATaa                >  1:2601156/1‑149 (MQ=255)

                                                                                                                                       ttCAGACAACAGCGGTTCCGCTGGAGGCTCAGATAGCGGCGGATTTAGCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCATTTTTAAATCAGATaaa               >  1:636186/1‑149 (MQ=255)

                                                                                                                                         cAGACAACCGCGGGTCCGCTGGAGGCTGAGATAGCGGCGGATTTAGCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCATTTTTAAATCAGATAAagt             <  2:2450533/149‑1 (MQ=255)

                                                                                                                                         cAGACAACAGCGGTTCCGCTGGAGGCTCAGATAGCGGCGGATTTAGCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCATTTTTAAATCAGATAAagt             >  1:2491080/1‑149 (MQ=255)

                                                                                                                                          aGACAACAGCGGTTCCGCTGGAGGCTCAGATAGCGGCGGATTTAGCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCATTTTTAAATCAGATAAagtc            >  2:4360947/1‑149 (MQ=255)

                                                                                                                                          aGACAACAGCGGTTCCGCTGGAGGCTCAGATAGCGGCGGATTTAGCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCATTTTTAAATCAGATAAagtc            >  2:5097175/1‑149 (MQ=255)

                                                                                                                                          aGACAACAGCGGTTCCGCTGGAGGCTCAGATAGCGGCGGATTTAGCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCATTTTTAAATCAGATAAagtc            <  1:1174784/149‑1 (MQ=255)

                                                                                                                                           gACAACAGCGGTTCCGCTGGAGGCTCAGATAGCGGCGGATTTAGCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATTCCAGCCCGGAGGCTGGCATTTTTAAATCAGATAAagtca           >  1:2029556/1‑149 (MQ=255)

                                                                                                                                              aacaGCGGTTCCGCTGGAGGCTCAGATAGCGGCGGATTTAGCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCATTTTTAAATCAGATAAagtcagtc        <  1:1336228/149‑1 (MQ=255)

                                                                                                                                                 aGCTGTTCCGCTGGAGGCTCAGATAGCGGCGGATTTAGCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTa     >  2:4032107/1‑149 (MQ=255)

                                                                                                                                                    ggTTCCGCTGGAGGCTCAGATAGCGGCGGATTTAGCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGc  <  1:4935708/149‑1 (MQ=255)

TCTTTTAGATTTATTCCTGTTCTCCGATAAAATTTTTTCCATTGCTGTATTTTCCTTCTTTTTTACTTTCACCATATTTATGGTCTTTACCTGTTTATGTGTACTTCAGAAAAGAGCATCTGGTAGAAGTTATCATTCAGACAACAGCGGTTCCGCTGGAGGCTCAGATAGCGGCGGATTTAGCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGC  >  CP009273/2899711‑2900007

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`