Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,931,717 C→A 88.4% N307K (AAC→AAA)  fucK → L‑fuculokinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,931,7170CA88.4% 905.5 / 114.0 316N307K (AAC→AAA) fucKL‑fuculokinase
Reads supporting (aligned to +/- strand):  ref base C (24/13);  new base A (156/122);  total (180/136)
Fisher's exact test for biased strand distribution p-value = 3.78e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.79e-01

Only 100 of 318 total aligned reads displayed.

GGCGCTGGTGCTGAACAAAATGAACCCGTGCTCTCTTCCGGTACATGGGAAATTTTAATGGTTCGCAGCGCCCAGGTTGATACTTCGCTGTTAAGTCAGTACGCCGGTTCCACCTGCGAACTGGATAGCCAGGCAGGGTTGTATAACCCAGGTATGCAATGGCTGGCATCCGGCGTGCTGGAATGGGTGAGAAAACTGTTCTGGACGGCTGAAACACCCTGGCAAATGTTGATTGAAGAAGCTCGTCTGATCGCGCCTGGCGCGGATGGCGTAAAAATGCAGTGTGATTTA  >  CP009273/2931571‑2931861
                                                                                                                                                  |                                                                                                                                                
 gcgcTGGTGCTGAACAAAATGAACCCGTGCTCTCTTCCGGTACATGGGAAATTTTAATGGTTCGCAGCGCCCAGGTTGATACTTCGCTGTTAAGTCAGTACGCCGGTTCCACCTGCGAACTGGATAGCCAGGCAGGGTTGTATAAACCa                                                                                                                                               <  1:253242/149‑1 (MQ=255)
  cgcTGGTGCTGAACAAAATGAACCCGTGCTCTCTTCCGGTACATGGGAAATTTTAATGGTTCGCAGCGCCCAGGTTGATACTTCGCTGTTAAGTCAGTACGCCGGTTCCACCTGCGAACTGGATAGCCAGGCAGGGTTGTATAACCCAg                                                                                                                                              <  1:2201674/149‑1 (MQ=255)
   gcTGGTGCTGAACAAAATGAACCCGTGCTCTCTTCCGGTACATGGGAAATTTTAATGGTTCGCAGCGCCCAGGTTGATACTTCGCTGTTAAGTCAGTACGCCGGTTCCACCTGCGAACTGGATAGCCAGGCAGGGTTGTATAAACCAgg                                                                                                                                             <  1:4221312/149‑1 (MQ=255)
   gcTGGTGCTGAACAAAATGAACCCGTGCTCTCTTCCGGTACATGGGAAATTTTAATGGTTCGCAGCGCCCAGGTTGATACTTCGCTGTTAAGTCAGTACGCCGGTTCCACCTGCGAACTGGATAGCCAGGCAGGGTTGTATAAACCAgg                                                                                                                                             <  1:4580538/149‑1 (MQ=255)
    cTGGTGCTGAACAAAATGAACCCGTGCTCTCTTCCGGTACATGGGAAATTTTAATGGTTCGCAGCGCCCAGGTTGATACTTCGCTGTTAAGTCAGTACGCCGGTTCCACCTGCGAACTGGATAGCCAGGCAGGGTTGTATAAACCAGGt                                                                                                                                            <  1:2404844/149‑1 (MQ=255)
        tGCTGAACAAAATGAACCCGTGCTCTCTTCCGGTACATGGGAAATTTTAATGGTTCGCAGCGCCCAGGTTGATACTTCGCTGTTAAGTCAGTACGCCGGTTCCACCTGCGAACTGGATAGCCAGGCAGGGTTGTATAAACCAGGTATGc                                                                                                                                        <  1:402428/149‑1 (MQ=255)
          cTGAACAAAATGAACCCGTGCTCTCTTCCGGTACATGGGAAATTTTAATGGTTCGCAGCGCCCAGGTTGATACTTCGCTGTTAAGTCAGTACGCCGGTTCCACCTGCAAACTGGATAGCCAGGCAGGGTTGTATAAACCAGGTATGCaa                                                                                                                                      <  1:2130248/149‑1 (MQ=255)
           tGAACAAAATGAACCCGTGCTCTCTTCCGGTACATGGGAAATTTTAATGGTTCGCAGCGCCCAGGTTGATACTTCGCTGTTAAGTCAGTACGCCGGTTCCACCTGCGAACTGGATAGCCAGGCAGGGTTGTATAAACCAGGTATGCAAt                                                                                                                                     <  1:3678352/149‑1 (MQ=255)
             aaCAAAATGAACCCGTGCTCTCTTCCGGTACATGGGAAATTTTAATGGTTCGCAGCGCCCAGGTTGATACTTCGCTGTTAAGTCAGTACGCCGGTTCCACCTGCGAACTGGATAGCCAGGCAGGGTTGTATAAACCAGGTATGCAAtgg                                                                                                                                   <  1:4359932/149‑1 (MQ=255)
              aCAAAATGAACCCGTGCTCTCTTCCGGTACATGGGAAATTTTAATGGTTCGCAGCGCCCAGGTTGATACTTCGCTGTTAAGTCAGTACGCCGGTTCCACCTGCGAACTGGATAGCCAGGCAGGGTTGTATAAACCAGGTATGCAAtggc                                                                                                                                  >  2:3592532/1‑149 (MQ=255)
               cAAAATGAACCCGTGCTCTCTTCCGGTACATGGGAAATTTTAATGGTTCGCAGCGCCCAGGTTGATACTTCGCTGTTAAGTCAGTACGCCGGTTCCACCTGCGAACTGGATAGCCAGGCAGGGTTGTATAAACCAGGTATGCAAtggct                                                                                                                                 >  1:3899743/1‑149 (MQ=255)
               cAAAATGAACCCGTGCTCTCTTCCGGTACATGGGAAATTTTAATGGTTCGCAGCGCCCAGGTTGATACTTCGCTGTTAAGTCAGTACGCCGGTTCCACCTGCGAACTGGATAGCCAGGCAGGGTTGTATAAACCAGGTATGCAAtggct                                                                                                                                 >  1:824010/1‑149 (MQ=255)
                 aaaTGAACCCGTGCTCTCTTCCGGTACATGGGAAATTTTAATGGTTCGCAGCGCCCAGGTTGATACTTCGCTGTTAAGTCAGTACGCCGGTTCCACCTGCGAACTGGATAGCCAGGCAGGGTTGTATAAACCAGGTATGCAAtggctgg                                                                                                                               <  1:4143153/149‑1 (MQ=255)
                 aaaTGAACCCGTGCTCTCTTCCGGTACATGGGAAATTTTAATGGTTCGCAGCGCCCAGGTTGATACTTCGCTGTTAAGTCAGTACGCCGGTTCCACCTGCGAACTGGATAGCCAGGCAGGGTTGTATAAACCAGGTATGCAAtggctgg                                                                                                                               >  2:4499342/1‑149 (MQ=255)
                 aaaTGAACCCGTGCTCTCTTCCGGTACATGGGAAATTTTAATGGTTCGCAGCGCCCAGGTTGATACTTCGCTGTTAAGTCAGTACGCCGGTTCCACCTGCGAACTGGATAGCCAGGCAGGGTTGTATAAACCAGGTATGCAAtggctga                                                                                                                               >  1:1208071/1‑148 (MQ=255)
                  aaTGAACCCGTGCTCTCTTCCGGTACATGGGAAATTTTAATGGTTCGCAGCGCCCAGGTTGATACTTCGCTGTTAAGTCAGTACGCCGGTTCCACCTGCGAACTGGATAGCCAGGCAGGGTTGTATAACCCAGGTATGCAAtggctggc                                                                                                                              >  1:1484190/1‑149 (MQ=255)
                  aaTGAACCCGTGCTCTCTTCCGGTACATGGGAAATTTTAATGGTTCGCAGCGCCCAGGTTGATACTTCGCTGTTAAGTCAGTACGCCGGTTCCACCTGCGAACTGGATAGCCAGGCAGGGTTGTATAAACCAGGTATGCAAtggctggc                                                                                                                              <  1:2836805/149‑1 (MQ=255)
                     gAACCCGTGCTCTCTTCCGGTACATGGGAAATTTTAATGGTTCGCAGCGCCCAGGTTGATACTTCGCTGTTAAGTCAGTACGCCGGTTCCACCTGCGAACTGGATAGCCAGGCAGGGTTGTATAAACCAGGTATGCAATGGCTGGCATc                                                                                                                           <  1:1396999/149‑1 (MQ=255)
                      aaCCCGTGCTCTCTTCCGGTACATGGGAAATTTTAATGGTTCGCAGCGCCCAGGTTGATACTTCGCTGTTAAGTCAGTACGCCGGTTCCACCTGCGAACTGGATAGCCAGGCAGGGTTGTATAACCCAGGTATGCAATGGCTGGAATcc                                                                                                                          >  1:3399660/1‑149 (MQ=255)
                      aaCCCGTGCTCTCTTCCGGTACATGGGAAATTTTAATGGTTCGCAGCGCCCAGGTTGATACTTCGCTGTTAAGTCAGTACGCCGGTTCCACCTGCGAACTGGATAGCCAGGCAGGGTTGTATAAACCAGGTATGCAATGGCTGGCATcc                                                                                                                          <  2:1577032/149‑1 (MQ=255)
                           gTGCTCTCTTCCGGTACATGGGAAATTTTAATGGTTCGCAGCGCCCAGGTTGATACTTCGCTGTTAAGTCAGTACGCCGGTTCCACCTGCGAACTGGATAGCCAGGCAGGGTTGTATAAACCAGGTATGCAATGGCTGGCATCCGGCGt                                                                                                                     <  2:1253451/149‑1 (MQ=255)
                           gTGCTCTCTTCCGGTACATGGGAAATTTTAATGGTTCGCAGCGCCCAGGTTGATACTTCGCTGTTAAGTCAGTACGCCGGTTCCACCTGCGAACTGGATAGCCAGGCAGGGTTGTATAAACCAGGTATGCAATGGCTGGCATCCGGCGt                                                                                                                     >  1:3203500/1‑149 (MQ=255)
                           gTGCTCTCTTCCGGTACATGGGAAATTTTAATGGTTCGCAGCGCCCAGGTTGATACTTCGCTGTTAAGTCAGTACGCCGGTTCCACCTGCGAACTGGATAGCCAGGCAGGGTTGTATAAACCAGGTATGCAATGGCTGGCATCCGGCGt                                                                                                                     >  1:1006339/1‑149 (MQ=255)
                            tGCTCTCTTCCGGTACATGGGAAATTTTAATGGTTCGCAGCGCCCAGGTTGATACTTCGCTGTTAAGTCAGTACGCCGGTTCCACCTGCGAACTGGATAGCCAGGCAGGGTTGTATAAACCAGGTATGCAATGGCTGGCATCCGGCGTg                                                                                                                    >  1:1101586/1‑149 (MQ=255)
                              ctctctTCCGGTACATGGGAAATTTTAATGGTTCGCAGCGCCCAGGTTGATACTTCGCTGTTAAGTCAGTACGCCGGTTCCACCTGCGAACTGGATAGCCAGGCAGGGTTGTATAACCCAGGTATGCAATGGCTGGCATCCGGCGTGCt                                                                                                                  >  1:1357194/1‑149 (MQ=255)
                                ctctTCCGGTACATGGGAAATTTTAATGGTTCGCAGCGCCCAGGTTGATACTTCGCTGTTAAGTCAGTACGCCGGTTCCACCTGCGAACTGGATAGCCAGGCAGGGTTGTATAAACCAGGTATGCAATGGCTGGCATCCGGCGTGCTgg                                                                                                                <  2:2064488/149‑1 (MQ=255)
                                  ctTCCGGTACATGGGAAATTTTAATGGTTCGCAGCGCCCAGGTTGATACTTCGCTGTTAAGTCAGTACGCCGGTTCCACCTGCGAACTGGATAGCCAGGCAGGGTTGTATAAACCAGGTATGCAATGGCTGGCATCCGGCGTGCTGGaa                                                                                                              <  1:449265/149‑1 (MQ=255)
                                       ggTACATGGGAAATTTTAATGGTTCGCAGCGCCCAGGTTGATACTTCGCTGTTAAGTCAGTACGCCGGTTCCACCTGCGAACTGGATAGCCAGGCAGGGTTGTATAAACCAGGTATGCAATGGCTGGCATCCGGCGTGCTGGAATGGGt                                                                                                         >  2:1163659/1‑149 (MQ=255)
                                       ggTACATGGGAAATTTTAATGGTTCGCAGCGCCCAGGTTGATACTTCGCTGTTAAGTCAGTACGCCGGTTCCACCTGCGAACTGGATAGCCAGGCAGGGTTGTATAAACCAGGTATGCAATGGCTGGCATCCGGCGTGCTGGAATGGGt                                                                                                         >  2:3266677/1‑149 (MQ=255)
                                       ggTACATGGGAAATTTTAATGGTTCGCAGCGCCCAGGTTGATACTTCGCTGTTAAGTCAGTACGCCGGTTCCACCTGCGAACTGGATAGCCAGGCAGGGTTGTATAAACCAGGTATGCAATGGCTGGCATCCGGCGTGCTGGAATGGGt                                                                                                         >  2:3784065/1‑149 (MQ=255)
                                       ggTACATGGGAAATTTTAATGGTTCGCAGCGCCCAGGTTGATACTTCGCTGTTAAGTCAGTACGCCGGTTCCACCTGCGAACTGGATAGCCAGGCAGGGTTGTATAAACCAGGTATGCAATGGCTGGCATCCGGCGTGCTGGAATGGGt                                                                                                         >  2:4726985/1‑149 (MQ=255)
                                         tACATGGGAAATTTTAATGGTTCGCAGCGCCCAGGTTGATACTTCGCTGTTAAGTCAGTACGCCGGTTCCACCTGCGAACTGGATAGCCAGGCAGGGTTGTATAAACCAGGTATGCAATGGCTGGCATCCGGCGTGCTGGACTGGGTga                                                                                                       >  2:3751169/1‑149 (MQ=255)
                                          aCATGGGAAATTTTAATGGTTCGCAGCGCCCAGGTTGATACTTCGCTGTTAAGTCAGTACGCCGGTTCCACCTGCGAACTGGATAGCCAGGCAGGGTTGTATAAACCAGGTATGCAATGGCTGGCATCCGGCGTGCTGGAATGGGTgag                                                                                                      >  1:355689/1‑149 (MQ=255)
                                            aTGGGAAATTTTAATGGTTCGCAGCGCCCAGGTTGATACTTCGCTGTTAAGTCAGTACGCCGGTTCCACCTGCGAACTGGATAGCCAGGCAGGGTTGTATAAACCAGGTATGCAATGGCTGGCATCCGGCGTGCTGGCATGGGTgagac                                                                                                    >  2:1686996/1‑148 (MQ=255)
                                             tGGGAAATTTTAATGGTTCGCAGCGCCCAGGTTGATACTTCGCTGTTAAGTCAGTACGCCGGTTCCACCTGCGAACTGGATGGCCAGGCAGGGTTGTATAAACCAGGTATGCAATGGCTGGCATCCGGCGTGCTGGAATGGGTGAGaaa                                                                                                   >  2:3713238/1‑149 (MQ=255)
                                             tGGGAAATTTTAATGGTTCGCAGCGCCCAGGTTGATACTTCGCTGTTAAGTCAGTACGCCGGTTCCACCTGCGAACTGGATAGCCAGGCAGGGTTGTATACACCAGGTATGCAATGGCTGGCATCCGGCGTGCTGGAATGGGTGAGaaa                                                                                                   >  2:5028704/1‑149 (MQ=255)
                                             tGGGAAATTTTAATGGTTCGCAGCGCCCAGGTTGATACTTCGCTGTTAAGTCAGTACGCCGGTTCCACCTGCGAACTGGATAGCCAGGCAGGGTTGTATAAACCAGGTATGCAATGGCTGGCATCCGGCGTGCTGGAATGGGTGAGaaa                                                                                                   >  2:220505/1‑149 (MQ=255)
                                              gggAAATTTTAATGGTTCGCAGCGCCCAGGTTGATACTTCGCTGTTAAGTCAGTACGCCGGTTCCACCTGCGAACTGGATAGCCAGGCAGGGTTGTATAAACCAGGTATGCAATGGCTGGCATCCGGCGTGCTGGAATGGGTGAGaaaa                                                                                                  >  2:914004/1‑149 (MQ=255)
                                                  aaTTTTAATGGTTCGCAGCGCCCAGGTTGATACTTCGCTGTTAAGTCAGTACGCCGGTTCCACCTGCGAACTGGATAGCCAGGCAGGGTTGTATAAACCAGGTATGCAATGGCTGGCATCCGGCGTGCTGGAATGGGTGAGAAAACTGt                                                                                              >  2:524989/1‑149 (MQ=255)
                                                  aaTTTTAATGGTTCGCAGCGCCCAGGTTGATACTTCGCTGTTAAGTCAGTACGCCGGTTCCACCTGCGAACTGGATAGCCAGGCAGGGTTGTATAAACCAGGTATGCAATGGCTGGCATCCGGCGTGCTGGAATGGGTGAGAAAACTGt                                                                                              <  2:834152/149‑1 (MQ=255)
                                                   aTTTTAATGGTTCGCAGCGCCCAGGTTTATACTTCGCTGTTAAGTCAGTACGCCGGTTCCACCTGCGAACTGGATAGCCAGGCAGGGTTGTATAATCCAGGTTTGCAATGGCTGGCATCGTGCGTTCTGGAATGGGTGAGAAAACTGtt                                                                                             <  1:4939064/149‑1 (MQ=255)
                                                   aTTTTAATGGTTCGCAGCGCCCAGGTTGATACTTCGCTGTTAAGTCAGTACGCCGGTTCCACCTGCGAACTGGATAGCCAGGCAGGGTTGTATAAACCAGGTATGCAATGGCTGGCATCCGGCGTGCTGGAATGGGTGAGAAAACTGtt                                                                                             >  1:4667570/1‑149 (MQ=255)
                                                    ttttAATGGTTCGCAGCGCCCAGGTTGATACTTCGCTGTTAAGTCAGTACGCCGGTTCCACCTGCGAACTGGATAGCCAGGCAGGGTTGTATAAACCAGGTATGCAATGGCTGGCATCCGGCGTGCTGGAATGGGTGAGAAAACTGTTc                                                                                            <  1:4219240/149‑1 (MQ=255)
                                                    ttttAATGGTTCGCAGCGCCCAGGTTGATACTTCGCTGTTAAGTCAGTACGCCGGTTCCACCTGCGAACTGGATAGCCAGGCAGGGTTGTATAAACCAGGTATGCAATGGCTGGCATCCGGCGTGCTGGAATGGGTGAGAAAACTGTTc                                                                                            >  1:1154719/1‑149 (MQ=255)
                                                    ttttAATGGTTCGCAGCGCCCAGGTTGATACTTCGCTGTTAAGTCAGTACGCCGGTTCCACCTGCGAACTGGATAGCCAGGCAGGGTTGTATAAACCAGGTATGCAATGGCTGGCATCCGGCGTGCTGGAATGGGTGAGAAAACTGTTc                                                                                            <  1:5144927/149‑1 (MQ=255)
                                                    ttttAATGGTTCGCAGCGCCCAGGTTGATACTTCGCTGTTAAGTCAGTACGCCGGTTCCACCTGCGAACTGGATAGCCAGGCAGGGTTGTATAAACCAGGTATGCAATGGCTGGCATCCGGCGTGCTGGAATGGGTGAGAAAACTGTTc                                                                                            <  2:4228535/149‑1 (MQ=255)
                                                      ttAATGGTTCGCAGCGCCCAGGTTGATACTTCGCTGTTAAGTCAGTACGCCGGTTCCACCTGCGAACTGGATAGCCAGGCAGGGTTGTATAAACCAGGTATGCAATGGCTGGCATCCGGCGTGCTGGAATGGGTGAGAAAACTGTTCTg                                                                                          >  2:865733/1‑149 (MQ=255)
                                                        aaTGGTTCGCAGCGCCCAGGTTGATACTTCGCTGTTAAGTCAGTACGCCGGTTCCACCTGCGAACTGGATAGCCAGGCAGGGTTGTATAAACCAGGTATGCAATGGCTGGCATCCGGCGTGCTGGAATGGGTGAGAAAACTGTTCTGGa                                                                                        >  1:279580/1‑149 (MQ=255)
                                                         aTGGTTCGCAGCGCCCAGGTTGATACTTCGCTGTTAAGTCAGTACGCCGGTTCCACCTGCGAACTGGATAGCCAGGCAGGGTTGTATAAACCAGGTATGCAATGGCTGGCATCCGGCGTGCTGGAATGGGTGAGAAAACTGTTCTGGAc                                                                                       >  2:3166053/1‑149 (MQ=255)
                                                         aTGGTTCGCAGCGCCCAGGTTGATACTTCGCTGTTAAGTCAGTACGCCGGTTCCACCTGCGAACTGGATAGCCAGGCAGGGTTGTATAAACCAGGTATGCAATGGCTGGCATCCGGCGTGCTGGAATGGGTGAGAAAACTGTTCTGGAc                                                                                       >  2:543029/1‑149 (MQ=255)
                                                            gTTCGCAGCGCCCAGGTTGATACTTCGCTGTTAAGTCAGTACGCCGGTTCCACCTGCGAACTGGATAGCCAGGCAGGGTTGTATAACCCAGGTATGCAATGGCTGGCATCCGGCGTGCTGGAATGGGTGAGAAAACTGTTCTGGACGGc                                                                                    <  2:4809241/149‑1 (MQ=255)
                                                            gTTCGCAGCGCCCAGGTTGATACTTCGCTGTTAAGTCAGTACGCCGGTTCCACCTGCGAACTGGATAGCCAGGCAGGGTTGTATAAACCAGGTATGCAATGGCTGGCATCCGGCGTGCTGGAATGGGTGAGAAAACTGTTCTGGACGGc                                                                                    >  1:1930815/1‑149 (MQ=255)
                                                                gCAGCGCCCAGGTTGATACTTCGCTGTTAAGTCAGTACGCCGGTTCCACCTGCGAACTGGATAGCCAGGCAGGGTTGTATAAACCAGGTATGCAATGGCTGGCATCCGGCGTGCTGGAATGGGTGAGAAAACTGTTCTGGACGGCTGaa                                                                                >  1:2891501/1‑149 (MQ=255)
                                                                   gcgcCCAGGTTGATACTTCGCTGTTAAGTCAGTACGCCGGTTCCACCTGCGAACTGGATAGCCAGGCAGGGTTGTATAAACCAGGTATGCAATGGCTGGCATCCGGCGTGCTGGAATGGGTGAGAAAACTGTTCTGGACGGCTGAAaca                                                                             >  1:977750/1‑149 (MQ=255)
                                                                       ccAGGTTGATACTTCGCTGTTAAGTCAGTACGCCGGTTCCACCTGCGAACTGGATAGCCAGGCAGGGTTGTATAAACCAGGTATGCAATGGCTGGCATCCGGCGTGCTGGAATGGGTGAGAAAACTGTTCTGGACGGCTGAAACACCCt                                                                         >  1:5215873/1‑149 (MQ=255)
                                                                        cAGGTTGATACTTCGCTGTTAAGTCAGTACGCCGGTTCCACCTGCGAACTGGATAGCCAGGCAGGGTTGTATAAACCAGGTATGCAATGGCTGGCATCCGGCGTGCTGGAATGGGTGAGAAAACTGTTCTGGACGGCTGAAACACCCTg                                                                        >  1:2027849/1‑149 (MQ=255)
                                                                        cAGGTTGATACTTCGCTGTTAAGTCAGTACGCCGGTTCCACCTGCGAACTGGATAGCCAGGCAGGGTTGTATAAACCAGGTATGCAATGGCTGGCATCCGGCGTGCTGGAATGGGTGAGAAAACTGTTCTGGACGGCTGAAACACCCTg                                                                        >  2:3047081/1‑149 (MQ=255)
                                                                        cAGGTTGATACTTCGCTGTTAAGTCAGTACGCCGGTTCCACCTGCGAACTGGATAGCCAGGCAGGGTTGTATAAACCAGGTATGCAATGGCTGGCATCCGGCGTGCTGGAATGGGTGAGAAAACTGTTCTGGACGGCTGAAACACCCTg                                                                        <  2:2965020/149‑1 (MQ=255)
                                                                        cAGGTTGATACTTCGCTGTTAAGTCAGTACGCCGGTTCCACCTGCGAACTGGATAGCCAGGCAGGGTTGTATAAACCAGGTATGCAATGGCTGGCATCCGGCGTGCTGGAATGGGTGAGAAAACTGTTCTGGACGGCTGAAACACCCTg                                                                        <  2:668155/149‑1 (MQ=255)
                                                                          ggTTGATACTTCGCTGTTAAGTCAGTACGCCGGTTCCACCTGCGAACTGGATAGCCAGGCAGGGTTGTATAAACCAGGTATGCAATGGCTGGCATCCGGCGTGCTGGAATGGGTGAGAAAACTGTTCTGGACGGCTGAAACACCCTGGc                                                                      >  1:3252282/1‑149 (MQ=255)
                                                                          ggTTGATACTTCGCTGTTAAGTCAGTACGCCGGTTCCACCTGCGAACTGGATAGCCAGGCAGGGTTGTATAAACCAGGTATGCAATGGCTGGCATCCGGCGTGCTGGAATGGGTGAGAAAACTGTTCTGGACGGCTGAAACACCCTGGc                                                                      <  2:4047862/149‑1 (MQ=255)
                                                                          ggTTGATACTTCGCTGTTAAGTCAGTACGCCGGTTCCACCTGCGAACTGGATAGCCAGGCAGGGTTGTATAAACCAGGTATGCAATGGCTGGCATCCGGCGTGCTGGAATGGGTGAGAAAACTGTTCTGGACGGCTGAAACACCCTGGc                                                                      <  2:2297331/149‑1 (MQ=255)
                                                                           gTTAATACTTCGCTGTTAAGTCAGTACGCCGGTTCCACCTGCGAACTGGATAGCCAGGCAGGGTTGTATAAACCAGGTATGCAATGGCTGGCATCCGGCGTGCTGGAATGGGTGAGAAAACTGTTCTGGACGGCTGAAACACCCTGGCa                                                                     >  1:785152/1‑149 (MQ=255)
                                                                              gATACTTCGCTGTTAAGTCAGTACGCCGGTTCCACCTGCGAACTGGATAGCCAGGCAGGGTTGTATAAACCAGGTATGCAATGGCTGGCATCCGGCGTGCTGGAATGGGTGAGAAAACTGTTCTGGACGGCTGAAACACCCTGGCAAAt                                                                  >  2:2178038/1‑149 (MQ=255)
                                                                                tACTTCGCTGTTAAGTCAGTACGCCGGTTCCACCTGCGAACTGGATAGCCAGGCAGGGTTGTATAAACCAGGTATGCAATGGCTGGCATCCGGCGTGCTGGAATGGGTGAGAAAACTGTTCTGGACGGCTGAAACACCCTGGCAAATGt                                                                <  2:3482430/149‑1 (MQ=255)
                                                                                   ttCGCTGTTAAGTCAGTACGCCGGTTCCACCTGCGAACTGGATAGCCAGGCAGGGTTGTATAACCCAGGTATGCAATGGCTGGCATCCGGCGTGCTGGAATGGGTGAGAAAACTGTTCTGGACGGCTGAAACACCCTGGCAAATGttga                                                             >  2:1965131/1‑149 (MQ=255)
                                                                                   ttCGCTGTTAAGTCAGTACGCCGGTTCCACCTGCGAACTGGATAGCCAGGCAGGGTTGTATAAACCAGGTATGCAATGGCTGGCATCCGGCGTGCTGGAATGGGTGAGAAAACTGTTCTGGACGGCTGAAACACCCTGGCAAATGttga                                                             >  1:2703151/1‑149 (MQ=255)
                                                                                   ttCGCTGTTAAGTCAGTACGCCGGTTCCACCTGCGAACTGGATAGCCAGGCAGGGTTGTATAAACCAGGTATGCAATGGCTGGCATCCGGCGTGCTGGAATGGGTGAGAAAACTGTTCTGGACGGCTGAAACACCCTGGCAAATGttga                                                             >  2:1384822/1‑149 (MQ=255)
                                                                                    tCGCTGTTAAGTCAGTACGCCGGTTCCACCTGCGAACTGGATAGCCAGGCAGGGTTGTATAAACCAGGTATGCAATGGCTGGCATCCGGCGTGCTGGAATGGGTGAGAAAACTGTTCTGGACGGCTGAAACACCCTGGCAAATGttgat                                                            >  2:2145176/1‑149 (MQ=255)
                                                                                       cTGTTAAGTCAGTACGCCGGTTCCACCTGCGAACTGGATAGCCAGGCAGGGTTGTATAAACCAGGTATGCAATGGCTGGCATCCGGCGTGCTGGAATGGGTGAGAAAACTGTTCTGGACGGCTGAAACACCCTGGCAAATGttgattga                                                         <  1:2982257/149‑1 (MQ=255)
                                                                                          ttAAGTCAGTACGCCGGTTCCACCTGCGAACTGGATAGCCAGGCAGGGTTGTATAAACCAGGTATGCAATGGCTGGCATCCGGCGTGCTGGAATGGGTGAGAAAACTGTTCTGGACGGCTGAAACACCCTGGCAAATGTTGATTgaaga                                                      <  2:2848182/149‑1 (MQ=255)
                                                                                               tCAGTACGCCGGTTCCACCTGCGAACTGGATAGCCAGGCAGGGTTGTATAAACCAGGTATGCAATGGCTGGCATCCGGCGTGCTGGAATGGGTGAGAAAACTGTTCTGGACGGCTGAAACACCCTGGCAAATGTTGATTGAAGAAGCTc                                                 <  2:4942181/149‑1 (MQ=255)
                                                                                                 aGTACGCCGGTTCCACCTGCGAACTGGATAGCCAGGCAGGGTTGTATAAACCAGGTATGCAATGGCTGGCATCCGGCGTGCTGGAATGGGTGAGAAAACTGTTCTGGACGGCTGAAACACCCTGGCAAATGTTGATTGAAGAAGCTCGt                                               <  1:1115947/149‑1 (MQ=255)
                                                                                                 aGTACGCCGGTTCCACCTGCGAACTGGATAGCCAGGCAGGGTTGTATAAACCAGGTATGCAATGGCTGGCATCCGGCGTGCTGGAATGGGTGAGAAAACTGTTCTGGACGGCTGAAACACCCTGGCAAATGTTGATTGAAGAAGCTCGt                                               <  1:3008547/149‑1 (MQ=255)
                                                                                                   tACGCCGGTTCCACCTGCGAACTGGATAGCCAGGCAGGGTTGTATAAACCAGGTATGCAATGGCTGGCATCCGGCGTGCTGGAATGGGTGAGAAAACTGTTCTGGACGGCTGAAACACCCTGGCAAATGTTGATTGAAGAAGCTCGTCt                                             >  1:1598168/1‑149 (MQ=255)
                                                                                                      gCCGGTTCCACCTGCGAACTGGATAGCCAGGCAGGGTTGTATAAACCAGGTATGCAATGGCTGGCATCCGGCGTGCTGGAATGGGTGAGAAAACTGTTCTGGACGGCTGAAACACCCTGGCAAATGTTGATTGAAGAAGCTCGTCTGAt                                          <  2:2579299/149‑1 (MQ=255)
                                                                                                           ttCCACCTGCGAACTGGATAGCCAGGCAGGGTTGTATAAACCAGGTATGCAATGGCTGGCATCCGGCGTGCTGGAATGGGTGAGAAAACTGTTCTGGACGGCTGAAACACCCTGGCAAATGTTGATTGAAGAAGCTCGTCTGATCgcgc                                     >  1:1268127/1‑149 (MQ=255)
                                                                                                              cACCTGCGAACTGGATAGCCAGGCAGGGTTGTATAAACCAGGTATGCAATGGCTGGCATCCGGCGTGCTGGAATGGGTGAGAAAACTGTTCTGGACGGCTGAAACACCCTGGCACATGTTGATTGAAGAAGCTCGTCTGATCGCGCCTg                                  >  1:1606868/1‑149 (MQ=255)
                                                                                                              cACCTGCGAACGGGATAGCCAGGCAGGGTTGTATAAACCAGGTATGCAATGGCTGGCATCCGGCGTGCTGGAATGGGTGAGAAAACTGTTCTGGACGGCTGAAACACCCTGGCAAATGTTGATTGAAGAAGCTCGTCTGATCGCGCCTg                                  <  2:4553039/149‑1 (MQ=255)
                                                                                                               aCCTGCGAACTGGATAGCCAGGCAGGGTTGTATAAACCAGGTATGCAATGGCTGGCATCCGGCGTGCTGGAATGGGTGAGAAAACTGTTCTGGACGGCTGAAACACCCTGGCAAATGTTGATTGAAGAAGCTCGTCTGATCGCGCCTgg                                 >  1:3501207/1‑149 (MQ=255)
                                                                                                                 cTGCGAACTGGATAGCCAGGCAGGGTTGTATAAACCAGGTATGCAATGGCTGGCATCCGGCGTGCTGGAATGGGTGAGAAAACTGTTCTGGACGGCTGAAACACCCTGGCAAATGTTGAGTGAAGAAGCTCGTCTGTTCGCGCCTGgcg                               >  2:1849124/1‑149 (MQ=255)
                                                                                                                   gCGAACTGGATAGCCAGGCAGGGTTGTATAAACCAGGTATGCAATGGCTGGCATCCGGCGTGCTGGAATGGGTGAGAAAACTGTTCTGGACGGCTGAAACACCCTGGCAAATGTTGATTGAAGAAGCTCGTCTGATCGCGCCTGGcgcg                             >  2:366498/1‑149 (MQ=255)
                                                                                                                        cTGGATAGCCAGGCAGGGTTGTATAAACCAGGTATGCAATGGCTGGCATCCGGCGTGCTGGAATGGGTGAGAAAACTGTTCTGGACGGCTGAAACACCCTGGCAAATGTTGATTGAAGAAGCTCGTCTGATCGCGCCTGGCGCGGATgg                        <  1:2651296/149‑1 (MQ=255)
                                                                                                                        cTGGATAGCCAGGCAGGGTTGTATAAACCAGGTATGCAATGGCTGGCATCCGGCGTGCTGGAATGGGTGAGAAAACTGTTCTGGACGGCTGAAACACCCTGGCAAATGTTGATTGAAGAAGCTCGTCTGATCGCGCCTGGCGCGGATgg                        >  1:3796749/1‑149 (MQ=255)
                                                                                                                           gATAGCCAGGCAGGGTTGTATAAACCAGGTATGCAATGGCTGGCATCCGGCGTGCTGGAATGGGTGAGAAAACTGTTCTGGACGGCTGAAACACCCTGGCAAATGTTGATTGAAGAAGCTCGTCTGATCGCGCCTGGCGCGGATGGCGt                     <  1:582754/149‑1 (MQ=255)
                                                                                                                           gATAGCCAGGCAGGGTTGTATAAACCAGGTATGCAATGGCTGGCATCCGGCGTGCTGGAATGGGTGAGAAAACTGTTCTGGACGGCTGAAACACCCTGGCAAATGTTGATTGAAGAAGCTCGTCTGATCGCGCCTGGCGCGGATGGCGt                     <  2:2219204/149‑1 (MQ=255)
                                                                                                                              aGCCAGGCAGGGTTGTATAAACCAGGTATGCACTGGCTGGCATCCGGCGTGCTGGAATGGGTGAGAAAACTGTTCTGGACGGCTGAAACACCCTGGCAAATGTTGATTGAAGAAGCTCGTCTGATCGCGCCTGGCGCGGATGGCGTaaa                  >  2:4087423/1‑149 (MQ=255)
                                                                                                                              aGCCAGGCAGGGTTGTATAAACCAGGTATGCAATGGCTGGCATCCGGCGTGCTGGAATGGGTGAGAAAACTGTTCTGGACGGCTGAAACACCCTGGCAAATGTTGATTGAAGAAGCTCGTCTGATCGCGCCTGGCGCGGATGGCGTaaa                  >  1:2320053/1‑149 (MQ=255)
                                                                                                                              aGCCAGGCAGGGTTGTATAAACCAGGTATGCAATGGCTGGCATCCGGCGTGCTGGAATGGGTGAGAAAACTGTTCTGGACGGCTGAAACACCCTGGCAAATGTTGATTGAAGAAGCTCGTCTGATCGCGCCTGGCGCGGATGGCGTaaa                  >  2:1457652/1‑149 (MQ=255)
                                                                                                                               gCCAGGCAGGGTTGTATAAACCAGGTATGCAATGGCTGGCATCCGGCGTGCTGGAATGGGTGAGAAAACTTTTCTGGACGGCTGAAACACCCTGGCAAATGTTGATTGAAGAAGCTCGTCTGATCGCGCCTGGCGCGGATGGCGTaaaa                 >  2:686019/1‑149 (MQ=255)
                                                                                                                                 caggcaggGTTGTATAACCCAGGTATGCAATGGCTGGCATCCGGCGTGCTGGAATGGGTGAGAAAACTGTTCTGGACGGCTGAAACACCCTGGCAAATGTTGATTGAAGAAGCTCGTCTGATCGCGCCTGGCGCGGATGGCGTAAAAAt               >  1:1026109/1‑149 (MQ=255)
                                                                                                                                 caggcaggGTTGTATAAACCAGGTATGCAATGGCTGGCATCCGGCGTGCTGGAATGGGTGAGAAAACTGTTCTGGACGGCTGAAACACCCTGGCAAATGTTGATTGAAGAAGCTCGTCTGATCGCGCCTGGCGCGGATGGCGTAAAAAt               <  1:4759420/149‑1 (MQ=255)
                                                                                                                                  aggcaggGTTGTATAAACCAGGTATGCAATGGCTGGCATCCGGCGTGCTGGAATGGGTGAGAAAACTGTTCTGGACGGCTGAAACACCCTGGCAAATGTTGATTGAAGAAGCTCGTCTGATCGCGCCTGGCGCGGATGGCGTAAAAATg              <  1:524989/149‑1 (MQ=255)
                                                                                                                                   ggcaggGTTGTATAAACCAGGTATGCAATGGCTGGCATCCGGCGTGCTGGAATGGGTGAGAAAACTGTTCTGGACGGCTGAAACACCCTGGCAAATGTTGATTGAAGAAGCTCGTCTGATCGCGCCTGGCGCGGATGGCGTAAAAATGc             >  2:3471186/1‑149 (MQ=255)
                                                                                                                                   ggcaggGTTGTATAAACCAGGTATGCAATGGCTGGCATCCGGCGTGCTGGAATGGGTGAGAAAACTGTTCTGGACGGCTGAAACACCCTGGCAAATGTTGATTGAAGAAGCTCGTCTGATCGCGCCTGGCGCGGATGGCGTAAAAATGc             >  2:3336100/1‑149 (MQ=255)
                                                                                                                                   ggcaggGTTGTATAAACCAGGTATGCAATGGCTGGCATCCGGCGTGCTGGAATGGGTGAGAAAACTGTTCTGGACGGCTGAAACACCCTGGCAAATGTTGATTGAAGAAGCTCGTCTGAACGCGCCTGGCTCGTATGGCGTAAAAATGc             <  2:385365/149‑1 (MQ=255)
                                                                                                                                       gggTTGTATAAACCAGGTATGCAATGGCTGGCATCCGGCGTGCTGGAATGGGTGAGAAAACTGTTCTGGACGGCTGAAACACCCTGGCAAATGTTGATTGAAGAAGCTCGTCTGATCGCGCCTGGCGCGGATGGCGTAAAAATGCAgtg         >  1:732996/1‑149 (MQ=255)
                                                                                                                                        ggTTGTATAAACCAGGTATGCAATGGCTGGCATCCGGCGTGCTGGAATGGGTGAGAAAACTGTTCTGGACGGCTGAAACACCCTGGCAAATGTTGATTGAAGAAGCTCGTCTGATCGCGCCTGGCGCGGATGGCGTAAAAATGCAgtgt        >  2:2424286/1‑149 (MQ=255)
                                                                                                                                         gTTGTATAAACCAGGTATGCAATGGCTGGCATCCGGCGTGCTGGAATGGGTGAGAAAACTGTTCTGGACGGCTGAAACACCCTGGCAAATGTTGATTGAAGAAGCTCGTCTGATCGCGCCTGGCGCGGATGGCGTAAAAATGCAGtgtg       <  1:487323/149‑1 (MQ=255)
                                                                                                                                              ataAACCAGGTATGCAATGGCTGGCATCCGGCGTGCTGGAATGGGTGAGAAAACTGTTCTGGACGGCTGAAACACCCTGGCAAATGTTGATTGAAGAAGCTCGTCTGATCGCGCCTGGCGCGGATGGCGTAAAAATGCAGTGTGATTTa  <  1:5038577/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                
GGCGCTGGTGCTGAACAAAATGAACCCGTGCTCTCTTCCGGTACATGGGAAATTTTAATGGTTCGCAGCGCCCAGGTTGATACTTCGCTGTTAAGTCAGTACGCCGGTTCCACCTGCGAACTGGATAGCCAGGCAGGGTTGTATAACCCAGGTATGCAATGGCTGGCATCCGGCGTGCTGGAATGGGTGAGAAAACTGTTCTGGACGGCTGAAACACCCTGGCAAATGTTGATTGAAGAAGCTCGTCTGATCGCGCCTGGCGCGGATGGCGTAAAAATGCAGTGTGATTTA  >  CP009273/2931571‑2931861

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑