BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	2,988,774	C→A	27.7%	pseudogene (346/447 nt)	ygeN ←	pseudogene

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	2,988,774	0	C	A	27.7%	374.4 / 147.7	181	pseudogene (346/447 nt)	ygeN	pseudogene
Reads supporting (aligned to +/- strand): ref base C (69/61); new base A (28/22); total (98/83)
Fisher's exact test for biased strand distribution p-value = 7.42e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.40e-01

Only 100 of 184 total aligned reads displayed.

ATATTACAATTAGATTATATTCAAATCATTAAACTTGAGCCAGGGAGCTATAAGTCCTCAGGGCTTGCCAAAACAGAATTATTCATATCCAAAGAATGTGTTTCAAACATTTTTTCAACTAAATAATGTCTGGCTTGTTCGCAGATTTCGTTAACCTTGTCAGGGGAGAAACAATGATTATTAATTAAATATTGCTCACAATTATCCACAAATTCCTGCGGACTAAATTCAGCAATAAATTGATCGGTAAAAAACACAAAACGCTTATCATCATGAAATGTAATATGGACATTCCAT  >  CP009273/2988626‑2988922

  atTACAATTAGATTATATTCAAATCATTAAACTTGAGCCAGGGAGCTATAAGTCCTCAGGGCTTGCCAAAACAGAATTATTCATATCCAAAGACTGTGTTTCAAACATTTTTTCAACTAAATAATGTCTGGCTTGTTCGCAGATTTCGt                                                                                                                                                    <  1:4894630/149‑1 (MQ=255)

   ttACAATTAGATTATATTCAAATCATTAAACTTGAGCCAGGGAGCTATAAGTCCTCAGGGCTTGCCAAAACAGAATTATTCATATCCAAAGAATGTGTTTCAAACATTTTTTCAACTAAATAATGTCTGGCTTGTTCGCAGATTTCGtt                                                                                                                                                   >  2:1068166/1‑149 (MQ=255)

       aaTTAGATTATATTCAAATCATTAAACTTGAGCCAGGGAGCTATAAGTCCTCAGGGCTTGCCAAAACAGAATTATTCATATCCAAAGAATGTGTTTCAAACATTTTTTCAACTAAATAATGTCTGGCTTGTTCGCAGATTTCGTTAAcc                                                                                                                                               >  2:600439/1‑149 (MQ=255)

       aaTTAGATTATATTCAAATCATTAAACTTGAGCCAGGGAGCTATAAGTCCTCAGGGCTTGCCAAAACAGAATTATTCATATCCAAAGAATGTGTTTCAAACATTTTTTCAACTAAATAATGTCTGGCTTGTTCGCAGATTTCGTTAAcc                                                                                                                                               >  2:4387552/1‑149 (MQ=255)

        aTTAGATTATATTCAAATCATTAAACTTGAGCCAGGGAGCTATAAGTCCTCAGGGCTTGCCAAAACAGAATTATTCATATCCAAAGAATGTGTTTCAAACATTTTTTCAACTAAATAATGTCTGGCTTGTTCGCAGATTTCGTTAACCt                                                                                                                                              <  2:3205478/149‑1 (MQ=255)

        aTTAGATTATATTCAAATCATTAAACTTGAGCCAGGGAGCTATAAGTCCTCAGGGCTTGCCAAAACAGAATTATTCATATCCAAAGAATGTGTTTCAAACATTTTTTCAACTAAATAATGTCTGGCTTGTTCGCAGATTTCGTTAACCt                                                                                                                                              >  1:4882086/1‑149 (MQ=255)

          tAGATTATATTCAAATCATTAAACTTGAGCCAGGGAGCTATAAGTCCTCAGGGCTTGCCAAAACAGAATTATTCATATCCAAAGAATGTGTTTCAAACATTTTTTCAACTAAATAATGTCTGGCTTGTTCGCAGATTTCGTTAACCTTg                                                                                                                                            <  2:162141/149‑1 (MQ=255)

           aGATTATATTCAAATCATTAAACTTGAGCCAGGGAGCTATAAGTCCTCAGGGCTTGCCAAAACAGAATTATTCATATCCAAAGAATGTGTTTCAAACATTTTTTCAACTAAATAATGTCTGGCTTGTTCGCAGATTTCGTTAACCTTGt                                                                                                                                           >  2:5206463/1‑149 (MQ=255)

             aTTATATTCAAATCATTAAACTTGAGCCAGGGAGCTATAAGTCCTCAGGGCTTGCCAAAACAGAATTATTCATATCCAAAGAATGTGTTTCAAACATTTTTTCAACTAAATAATGTCTGGCTTGTTCGCAGATTTCGTTAACCTTGTCa                                                                                                                                         <  2:5016924/149‑1 (MQ=255)

             aTTATATTCAAATCATTAAACTTGAGCCAGGGAGCTATAAGTCCTCAGGGCTTGCCAAAACAGAATTATTCATATCCAAAGAATGTGTTTCAAACATTTTTTCAACTAAATAATGTCTGGCTTGTTCGCAGATTTAGTTAACCTTGTCa                                                                                                                                         <  2:1354234/149‑1 (MQ=255)

              ttATATTCAAATCATTAAACTTGAGCCAGGGAGCTATAAGTCCTCAGGGCTTGCCAAAACAGAATTATTCATATCCAAAGAATGTGTTTCAAACATTTTTTCAACTAAATAATGTCTGGCTTGTTCGCAGATTTCGTTAACCTTGTCAg                                                                                                                                        <  1:4513741/149‑1 (MQ=255)

               tataTTCAAATCATTAAACTTGAGCCAGGGAGCTATAAGTCCTCAGGGCTTGCCAAAACAGAATTATTCATATCCAAAGAATGTGTTTCAAACATTTTTTCAACTAAATAATGTCTGGCTTGTTCGCAGATTTCGTTAACCTTGTCAgg                                                                                                                                       <  1:843927/149‑1 (MQ=255)

                atatTCAAATCATTAAACTTGAGCCAGGGAGCTATAAGTCCTCAGGGCTTGCCAAAACAGAATTATTCATATCCAAAGAATGTGTTTCAAACATTTTTTCAACTAAATAATGTCTGGCTTGTTCGCAGATTTAGTTAACCTTGTCAggg                                                                                                                                      >  1:4244180/1‑149 (MQ=255)

                 tatTCAAATCATTAAACTTGAGCCAGGGAGCTATAAGTCCTCAGGGCTTGCCAAAACAGAATTATTCATATCCAAAGAATGTGTTTCAAACATTTTTTCAACTAAATAATGTCTGGCTTGTTCGCAGATTTCGTTAACCTTGTCAgggg                                                                                                                                     <  2:3372418/149‑1 (MQ=255)

                     cAAATCATTAAACTTGAGCCAGGGAGCTATAAGTCCTCAGGGCTTGCCAAAACAGAATTATTCATATCCAAAGAATGTGTTTCAAACATTTTTTCAACTAAATAATGTCTGGCTTGTTCGCAGATTTCGTTAACCTTGTCAGGGGAGaa                                                                                                                                 >  1:4932143/1‑149 (MQ=255)

                       aaTCATTAAACTTGAGCCAGGGAGCTATAAGTCCTCAGGGCTTGCCAAAACAGAATTATTCATATCCAAAGAATGTGTTTCAAACATTTTTTCAACTAAATAATGTCTGGCTTGTTCGCAGATTTCGTTAACCTTGTCAGGGGAGAAAc                                                                                                                               <  1:4912335/149‑1 (MQ=255)

                       aaTCATTAAACTTGAGCCAGGGAGCTATAAGTCCTCAGGGCTTGCCAAAACAGAATTATTCATATCCAAAGAATGTGTTTCAAACATTTTTTCAACTAAATAATGTCTGGCTTGTTCGCAGATTTAGTTAACCTTGTCAGGGGAGAAAc                                                                                                                               >  1:1881794/1‑149 (MQ=255)

                        aTCATTAAACTTGAGCCAGGGAGCTATAAGTCCTCAGGGCTTGCCAAAACAGAATTATTCATATCCAAAGAATGTGTTTCAAACATTTTTTCAACTAAATAATGTCTGGCTTGTTCGCAGATTTAGTTAACCTTGTCAGGGGAGAAACa                                                                                                                              <  1:3939159/149‑1 (MQ=255)

                        aTCATTAAACTTGAGCCAGGGAGCTATAAGTCCTCAGGGCTTGCCAAAACAGAATTATTCATATCCAAAGAATGTGTTTCAAACATTTTTTCAACTAAATAATGTCTGGCTTGTTCGCAGATTTAGTTAACCTTGTCAGGGGAGAAACa                                                                                                                              <  1:2389944/149‑1 (MQ=255)

                          cATTAAACTTGAGCCAGGGAGCTATAAGTCCTCAGGGCTTGCCAAAACAGAATTATTCATATCCAAAGAATGTGTTTCAAACATTTTTTCAACTAAATAATGTCTGGCTTGTTCGCAGATTTCGTTAACCTTGTCAGGGGAGAAACAAt                                                                                                                            <  2:3728175/149‑1 (MQ=255)

                            ttAAACTTGAGCCAGGGAGCTATAAGTCCTCAGGGCTTGCCAAAACAGAATTATTCATATCCAAAGAATGTGTTTCAAACATTTTTTCAACTAAATAATGTCTGGCTTGTTCGCAGATTTAGTTAACCTTGTCAGGGGAGAAACAATGa                                                                                                                          >  1:4529815/1‑149 (MQ=255)

                                aCTTGAGCCAGGGAGCTATAAGTCCTCAGGGCTTGCCAAAACAGAATTATTCATATCCAAAGAATGTGTTTCAAACATTTTTTCAACTAAATAATGTCTGGCTTGTTCGCAGATTTCGTTAACCTTGTCAGGGGAGAAACAATGattat                                                                                                                      <  2:3053968/149‑1 (MQ=255)

                                 cTTGAGCCAGGGAGCTATAAGTCCTCAGGGCTTGCCAAAACAGAATTATTCATATCCAAAGAATGTGTTTCAAACATTTTTTCAACTAAATAATGTCTGGCTTGTTCGCAGATTTCGTTAACCTTGTCAGGGGAGAAACAATGattatt                                                                                                                     >  1:2028844/1‑149 (MQ=255)

                                  ttGAGCCAGGGAGCTATAAGTCCTCAGGGCTTGCCAAAACAGAATTATTCATATCCAAAGAATGTGTTTCAAACATTTTTTCAACTAAATAATGTCTGGCTTGTTCGCAGATTTCGTTAACCTTGTCAGGGGAGAAACAATGAttatta                                                                                                                    <  2:1826479/149‑1 (MQ=255)

                                    gAGCCAGGGAGCTATAAGTCCTCAGGGCTTGCCAAAACAGAATTATTCATATCCAAAGAATGTGTTTCAAACATTTTTTCAACTAAATAATGTCTGGCTTGTTCGCAGATTTAGTTAACCTTGTCAGGGGAGAAACAATGATTattaat                                                                                                                  <  2:3648647/149‑1 (MQ=255)

                                      gCCAGGGAGCTATAAGTCCTCAGGGCTTGCCAAAACAGAATTATTCATATCCAAAGAATGTGTTTCAAACATTTTTTCAACTAAATAATGTCTGGCTTGTTCGCAGATTTCGTTAACCTTGTCAGGGGAGAAACAATGATTattaatta                                                                                                                >  2:4364227/1‑149 (MQ=255)

                                       ccAGGGAGCTATAAGTCCTCAGGGCTTGCCAAAACAGAATTATTCATATCCAAAGAATGTGTTTCAAACATTTTTTCAACTAAATAATGTCTGGCTTGTTCGCAGATTTCGTTAACATTGTCAGTGGAGAAACAATGATAAttaattaa                                                                                                               >  1:1211634/1‑149 (MQ=255)

                                         aGGGAGCTATAAGTCCTCAGGGCTTGCCAAAACAGAATTATTCATATCCAAAGAATGTGTTTCAAACATTTTTTCAACTAAATAATGTCTGGCTTGTTCGCAGATTTCGTTAACCTTGTCAGGGGAGAAACAATGATTATTAATTAAat                                                                                                             >  2:2199819/1‑149 (MQ=255)

                                         aGGGAGCTATAAGTCCTCAGGGCTTGCCAAAACAGAATTATTCATATCCAAAGAATGTGTTTCAAACATTTTTTCAACTAAATAATGTCTGGCTTGTTCGCAGATTTAGTTAACCTTGTCAGGGGAGAAACAATGATTATTAATTAAat                                                                                                             >  2:1585304/1‑149 (MQ=255)

                                          gggAGCTATAAGTCCTCAGGGCTTGCCAAAACAGAATTATTCATATCCAAAGAATGTGTTTCAAACATTTTTTCAACTAAATAATGTCTGGCTTGTTCGCAGATTTCGTTAACCTTGTCAGGGGAGAAACAATGATTATTAATTAAata                                                                                                            >  1:129477/1‑149 (MQ=255)

                                          gggAGCTATAAGTCCTCAGGGCTTGCCAAAACAGAATTATTCATATCCAAAGAATGTGTTTCAAACATTTTTTCAACTAAATAATGTCTGGCTTGTTCGCAGATTTAGTTAACCTTGTCAGGGGAGAAACAATGATTATTAATTAAata                                                                                                            >  2:4530578/1‑149 (MQ=255)

                                            gAGCTATAAGTCCTCAGGGCTTGCCAAAACAGAATTATTCATATCCAAAGAATGTGTTTCAAACATTTTTTCAACTAAATAATGTCTGGCTTGTTCGCAGATTTCGTTAACCTTGTCAGGGGAGAAACAATGATTATTAATTAAATAtt                                                                                                          >  1:3447095/1‑149 (MQ=255)

                                              gCTATAAGTCCTCAGGGCTTGCCAAAACAGAATTATTCATATCCAAAGAATGTGTTTCAAACATTTTTTCAACTAAATAATGTCTGGCTTGTTCGCAGATTTAGTTAACCTTGTCAGGGGAGAAACAATGATTATTAATTAAATATTGc                                                                                                        >  2:3200798/1‑149 (MQ=255)

                                                    aGTCCTCAGGGCTTGCCAAAACAGAATTATTCATATCCAAAGAATGTGTTTCAAACATTTTTTCAACTAAATAATGTCTGGCTTGTTCGCAGATTTCGTTAACCTTGTCAGGGGAGAAACAATGATTATTAATTAAATATTGCTCACaa                                                                                                  >  2:794802/1‑149 (MQ=255)

                                                    aGTCCTCAGGGCTTGCCAAAACAGAATTATTCATATCCAAAGAATGTGTTTCAAACATTTTTTCAACTAAATAATGTCTGGCTTGTTCGCAGATTTCGTTAACCTTGTCAGGGGAGAAACAATGATTATTAATTAAATATTGCTCACaa                                                                                                  <  2:5050078/149‑1 (MQ=255)

                                                         tCAGGGCTTGCCAAAACAGAATTATTCATATCCAAAGAATGTGTTTCAAACATTTTTTCAACTAAATAATGTCTGGCTTGTTCGCAGATTTCGTTAACCTTGTCAGGGGAGAAACAATGATTATTAATTAAATATTGCTCACAATTATc                                                                                             <  2:3511783/149‑1 (MQ=255)

                                                          cAGGGCTTGCCAAAACAGAATTATTCATATCCAAAGAATGTGTTTCAAACATTTTTTCAACTAAATAATGTCTGGCTTGTTCGCAGATTTCGTTAACCTTGTCAGGGGAGAAACAATGATTATTAATTAAATATTGCTCACAATTATcc                                                                                            <  1:3108884/149‑1 (MQ=255)

                                                          cAGGGCTTGCCAAAACAGAATTATTCATATCCAAAGAATGTGTTTCAAACATTTTTTCAACTAAATAATGTCTGGCTTGTTCGCAGATTTCGTTAACCTTGTCAGGGGAGAAACAATGATTATTAATTAAATATTGATCACAATTATcc                                                                                            >  2:2677078/1‑149 (MQ=255)

                                                           aGGGCTTGCCAAAACAGAATTATTCATATCCAAAGAATGTGTTTCAAACATTTTTTCAACTAAATAATGTCTGGCTTGTTCGCAGATTTCGTTAACCTTGTCAGGGGAGAAACAATGATTATTAATTAAATATTGCTCACAATTATCca                                                                                           <  1:1573023/149‑1 (MQ=255)

                                                             ggCTTGCCAAAACAGAATTATTCATATCCAAAGAATGTGTTTCAAACATTTTTTCAACTAAATAATGTCTGGCTTGTTCGCAGATTTCGTTAACCTTGTCAGGGGAGAAACAATGATTATTAATTAAATATTGCTCACAATTATCcaca                                                                                         <  2:5242014/149‑1 (MQ=255)

                                                                ttGCCAAAACAGAATTATTCATATCCAAAGAATGTGTTTCAAACATTTTTTCAACTAAATAATGTCTGGCTTGTTCGCAGATTTCGTTAACCTTGTCAGGGGAGAAACAATGATTATTAATTAAATATTGCTCACAATTATCCACAAAt                                                                                      <  2:4882086/149‑1 (MQ=255)

                                                                    cAAAACAGAATTATTCATATCCAAAGAATGTGTTTCAAACATTTTTTCAACTAAATAATGTCTGGCTTGTTCGCAGATTTCGTTAACCTTGTCAGGGGAGAAACAATGATTATTAATTAAATATTGCTCACAATTATCCACAAATTCCt                                                                                  >  1:1129465/1‑149 (MQ=255)

                                                                     aaaaCAGAATTATTCATATCCAAAGAATGTGTTTCAAACATTTTTTCAACTAAATAATGTCTGGCTTGTTCGCAGATTTCGTTAACCTTGTCAGGGGAGAAACAATGATTATTAATTAAATATTGCTCACAATTATCCACAAATTCCTg                                                                                 <  1:1563062/149‑1 (MQ=255)

                                                                        aCAGAATTATTCATATCCAAAGAATGTGTTTCAAACATTTTTTCAACTAAATAATGTCTGGCTTGTTCGCAGATTTCGTTAACCTTGTCAGGGGAGAAACAATGATTATTAATTAAATATTGCTCACAATTATCCACAAATTCCTGCgg                                                                              >  1:855935/1‑149 (MQ=255)

                                                                         cAGAATTATTCATATCCAAAGAATGTGTTTCAAACATTTTTTCAACTAAATAATGTCTGGCTTGTTCGCAGATTTCGTTAACCTTGTCAGGGGAGAAACAATGATTATTAATTAAATATTGCTCACAATTATCCACAAATTCCTGCGGa                                                                             >  1:4106529/1‑149 (MQ=255)

                                                                         cAGAATTATTCATATCCAAAGAATGTGTTTCAAACATTTTTTCAACTAAATAATGTCTGGCTTGTTCGCAGATTTCGTTAACCTTGTCAGGGGAGAAACAATGATTATTAATTAAATATTGCTCACAATTATCCACAAATTCCTGCGGa                                                                             >  1:3642068/1‑149 (MQ=255)

                                                                          aGAATTATTCATATCCAAAGAATGTGTTTCAAACATTTTTTCAACTAAATAATGTCTGGCTTGTTCGCAGATTTCGTTAACCTTGTCAGGGGAGAAACAATGATTATTAATTAAATATTGCTCACAATTATCCACAAATTCCTGCGGAc                                                                            >  1:1159342/1‑149 (MQ=255)

                                                                          aGAATTATTCATATCCAAAGAATGTGTTTCAAACATTTTTTCAACTAAATAATGTCTGGCTTGTTCGCAGATTTCGTTAACCTTGTCAGGGGAGAAACAATGATTATTAATTAAATATTGCTCACAATTATCCACAAATTCCTGCGGAc                                                                            <  1:547013/149‑1 (MQ=255)

                                                                              ttattCATATCCAAAGAATGTGTTTCAAACATTTTTTCAACTAAATAATGTCTGGCTTGTTCGCAGATTTCGTTAACCTTGTCAGGGGAGAAACAATGATTATTAATTAAATATTGCTCACAATTATCCACAAATTCCTGCGGACTaaa                                                                        >  2:171944/1‑149 (MQ=255)

                                                                               tattCATATCCAAAGAATGTGTTTCAAACATTTTTTCAACTAAATAATGTCTGGCTTGTTCGCAGATTTCGTTAACCTTGTCAGGGGAGAAACAATGATTATTAATTAAATATTGCTCACAATTATCCACAAATTCCTGCGGACTAAAt                                                                       <  2:2321975/149‑1 (MQ=255)

                                                                               tattCATATCCAAAGAATGTGTTTCAAACATTTTTTCAACTAAATAATGTCTGGCTTGTTCGCAGATTTAGTTAACCTTGTCAGGGGAGAAACAATGATTATTAATTAAATATTGCTCACAATTATCCACAAATTCCTGCGGACTAAAt                                                                       >  2:1192923/1‑149 (MQ=255)

                                                                                   cATATCCAAAGAATGTGTTTCAAACATTTTTTCAACTAAATAATGTCTGGCTTGTTCGCAGATTTCGTTAACCTTGTCAGGGGAGAAACAATGATTATTAATTAAATATTGCTCACAATTATCCACAAATTCCTGCGGACTAAATTCAg                                                                   <  1:3819602/149‑1 (MQ=255)

                                                                                   cATATCCAAAGAATGTGTTTCAAACATTTTTTCAACTAAATAATGTCTGGCTTGTTCGCAGATTTCGTTAACCTTGTCAGGGGAGAAACAATGATTATTAATTAAATATTGCTCACAATTATCCACAAATTCCTGCGGACTAAATTCAg                                                                   <  2:2173678/149‑1 (MQ=255)

                                                                                       tCCAAAGAATGTGTTTCAAACATTTTTTCAACTAAATAATGTCTGGCTTGTTCGCAGATTTCGTTAACCTTGTCAGGGGAGAAACAATGATTATTAATTAAATATTGCTCACAATTATCCACAAATTCCTGCGGACTAAATTCAGCAAt                                                               <  2:2028844/149‑1 (MQ=255)

                                                                                       tCCAAAGAATGTGTTTCAAACATTTTTTCAACTAAATAATGTCTGGCTTGTTCGCAGATTTAGTTAACCTTGTCAGGGGAGAAACAATGATTATTAATTAAATATTGCTCACAATTATCCACAAATTCCTGCGGACTAAATTCAGCAAt                                                               >  1:2804009/1‑149 (MQ=255)

                                                                                        ccAAAGAATGTGTTTCAAACATTTTTTCAACTAAATAATGTCTGGCTTGTTCGCAGATTTCGTTAACCTTGTCAGGGGAGAAACAATGATTATTAATTAAATATTGCTCACAATTATCCACAAATTCCTGCGGACTAAATTCAGCAATa                                                              >  1:5160867/1‑149 (MQ=255)

                                                                                          aaaGAATGTGTTTCAAACATTTTTTCAACTAAATAATGTCTGGCTTGTTCGCAGATTTCGTTAACCTTGTCAGGGGAGAAACAATGATTATTAATTAAATATTGCTCACAATTATCCACAAATTCCTGCGGACTAAATTCAGCAATaaa                                                            <  2:3905486/149‑1 (MQ=255)

                                                                                          aaaGAATGTGTTTCAAACATTTTTTCAACTAAATAATGTCTGGCTTGTTCGCAGATTTAGTTAACCTTGTCAGGGGAGAAACAATGATTATTAATTAAATATTGCTCACAATTATCCACAAATTCCTGCGGACTAAATTCAGCAATaaa                                                            >  1:4998664/1‑149 (MQ=255)

                                                                                          aaaGAATGTGTTTCAAACATTTTTTCAACTAAATAATGTCTGGCTTGTTCGCAGATTTAGTTAACCTTGTCAGGGGAGAAACAATGATTATTAATTAAATATTGCTCACAATTATCCACAAATTCCTGCGGACTAAATTCAGCAATaaa                                                            <  1:318688/149‑1 (MQ=255)

                                                                                             gAATGTGTTTCAAACATTTTTTCAACTAAATAATGTCTGGCTTGTTCGCAGATTTCGTTAACCTTGTCAGGGGAGAAACAATGATTATTAATTAAATATTGCTCACAATTATCCACAAATTCCTGCGGACTAAATTCAGCAATAAATTg                                                         >  2:1408394/1‑149 (MQ=255)

                                                                                             gAATGTGTTTCAAACATTTTTTCAACTAAATAATGTCTGGCTTGTTCGCAGATTTCGTTAACCTTGTCAGGGGAGAAACAATGATTATTAATTAAATATTGCTCACAATTATCCACAAATTCCTGCGGACTAAATTCAGCAATAAATTg                                                         >  1:1281404/1‑149 (MQ=255)

                                                                                              aaTGTGTTTCAAACATTTTTTCAACTAAATAATGTCTGGCTTGTTCGCAGATTTAGTTAACCTTGTCAGGGGAGAAACAATGATTATTAATTAAATATTGCTCACAATTATCCACAAATTCCTGCGGACTAAATTCAGCAATAAATTGa                                                        >  1:3418358/1‑149 (MQ=255)

                                                                                               aTGTGTTTCAAACATTTTTTCAACTAAATAATGTCTGGCTTGTTCGCAGATTTAGTTAACCTTGTCAGGGGAGAAACAATGATTATTAATTAAATATTGCTCACAATTATCCACAAATTCCTGCGGACTAAATTCAGCAATAAATTGAt                                                       <  2:1880972/149‑1 (MQ=255)

                                                                                                tgtgTTTCAAACATTTTTTCAACTAAATAATGTCTGGCTTGTTCGCAGATTTCGTTAACCTTGTCAGGGGAGAAACAATGATTATTAATTAAATATTGCTCACAATTATCCACAAATTCCTGCGGACTAAATTCAGCAATAAATTGATc                                                      <  1:4235363/149‑1 (MQ=255)

                                                                                                tgtgTTTCAAACATTTTTTCAACTAAATAATGTCTGGCTTGTTCGCAGATTTCGTTAACCTTGTCAGGGGAGAAACAATGATTATTAATTAAATATTGCTCACAATTATCCACAAATTCCTGCGGACTAAATTCAGCAATAAATTGATc                                                      <  2:3299262/149‑1 (MQ=255)

                                                                                                   gtTTCAAACATTTTTTCAACTAAATAATGTCTGGCTTGTTCGCAGATTTCGTTAACCTTGTCAGGGGAGAAACAATGATTATTAATTAAATATTGCTCACAATTATCCACAAATTCCTGCGGACTAAATTCAGCAATAAATTGATCgtt                                                   <  2:4815967/149‑3 (MQ=255)

                                                                                                   gtTTCAAACATTTTTTCAACTAAATAATGTCTGGCTTGTTCGCAGATTTCGTTAACCTTGTCAGGGGAGAAACAATGATTATTAATTAAATATTGCTCACAATTATCCACAAATTCCTGCGGACTAAATTCAGCAATAAATTGATCGGt                                                   <  2:4275883/149‑1 (MQ=255)

                                                                                                   gtTTCAAACATTTTTTCAACTAAATAATGTCTGGCTTGTTCGCAGATTTCGTTAACCTTGTCAGGGGAGAAACAATGATTATTAATTAAATATTGCTCACAATTATCCACAAATTCCTGCGGACTAAATTCAGCAATAAATTGATCGGt                                                   >  1:881591/1‑149 (MQ=255)

                                                                                                      tCAAACATTTTTTCAACTAAATAATGTCTGGCTTGTTCGCAGATTTCGTTAACCTTGTCAGGGGAGAAACAATGATTATTAATTAAATATTGCTCACAATTATCCACAAATTCCTGCGGACTAAATTCAGCAATAAATTGATCGGTaaa                                                >  1:4005074/1‑149 (MQ=255)

                                                                                                      tCAAACATTTTTTCAACTAAATAATGTCTGGCTTGTTCGCAGATTTCGTTAACCTTGTCAGGGGAGAAACAATGATTATTAATTAAATATTGCTCACAATTATCCACAAATTCCTGCGGACTAAATTCAGCAATAAATTGATCGGTaaa                                                >  1:4004990/1‑149 (MQ=255)

                                                                                                          aCATTTTTTCAACTAAATAATGTCTGGCTTGTTCGCAGATTTAGTTAACCTTGTCAGGGGAGAAACAATGATTATTAATTAAATATTGCTCACAATTATCCACAAATTCCTGCGGACTAAATTCAGCAATAAATTGATCGGTAAAAAac                                            <  2:4244146/149‑1 (MQ=255)

                                                                                                            aTTTTTTCAACTAAATAATGTCTGGCTTGTTCGCAGATTTCGTTAACCTTGTCAGGGGAGAAACAATGATTATTAATTAAATATTGCTCACAATTATCCACAAATTCCTGCGGACTAAATTCAGCAATAAATTGATCGGTAAAAAacac                                          >  1:2100858/1‑149 (MQ=255)

                                                                                                                 ttCAACTAAATAATGTCTGGCTTGTTCGCAGATTTCGTTAACCTTGTCAGGGGAGAAACAATGATTATTAATTAAATATTGCTCACAATTATCCACAAATTCCTGCGGACTAAATTCAGCAATAAATTGATCGGTAAAAAACACAAAAc                                     <  1:611928/149‑1 (MQ=255)

                                                                                                                  tCAACTAAATAATGTCTGGCTTGTTCGCAGATTTAGTTAACCTTGTCAGGGGAGAAACAATGATTATTAATTAAATATTGCTCACAATTATCCACAAATTCCTGCGGACTAAATTCAGCAATAAATTGATCGGTAAAAAACACAAAACg                                    >  2:4213310/1‑149 (MQ=255)

                                                                                                                    aaCTAAATAATGTCTGGCTTGTTCGCAGATTTCGTTAACCTTGTCAGGGGAGAAACAATGATTATTAATTAAATATTGCTCACAATTATCCACAAATTCCTGCGGACTAAATTCAGCAATAAATTGATCGGTAAAAAACACAAAACGCt                                  >  2:3807897/1‑149 (MQ=255)

                                                                                                                       tAAATAATGTCTGGCTTGTTCGCAGATTTAGTTAACCTTGTCAGGGGAGAAACAATGATTATTAATTAAATATTGCTCACAATTATCCACAAATTCCTGCGGACTAAATTCAGCAATAAATTGATCGGTAAAAAACACAAAACGCTTat                               >  1:2496492/1‑149 (MQ=255)

                                                                                                                        aaaTAATGTCTGGCTTGTTCGCAGATTTAGTTAACCTTGTCAGGGGAGAAACAATGATTATTAATTAAATATTGCTCACCATTATCCACAAATTCCTGCGGACTAAATTCAGCAATAAATTGATCGGTAAAAAACACAAAACGCTTatc                              >  2:53134/1‑149 (MQ=255)

                                                                                                                         aataatGTCTGGCTTGTTCGCAGATTTCGTTAACCTTGTCAGGGGAGAAACAATGATTATTAATTAAATATTGCTCACAATTATCCACAAATTCCTGCGGACTAAATTCAGCAATAAATTGATCGGTAAAAAACACAAAACGCTTatca                             >  2:1992268/1‑149 (MQ=255)

                                                                                                                           taatGTCTGGCTTGTTCGCAGATTTCGTTAACCTTGTCAGGGGAGAAACAATGATTATTAATTAAATATTGCTCACAATTATCCACAAATTCCTGCGGACTAAATTCAGCAATAAATTGATCGGTAAAAAACACAAAACGCTTatcatc                           >  2:1105437/1‑149 (MQ=255)

                                                                                                                           taatGTCTGGCTTGTTCGCAGATTTCGTTAACCTTGTCAGGGGAGAAACAATGATTATTAATTAAATATTGCTCACAATTATCCACAAATTCCTGCGGACTAAATTCAGCAATAAATTGATCGGTAAAAAACACAAAACGCTTatcatc                           >  2:2467765/1‑149 (MQ=255)

                                                                                                                           taatGTCTGGCTTGTTCGCAGATTTCGTTAACCTTGTCAGGGGAGAAACAATGATTATTAATTAAATATTGCTCACAATTATCCACAAATTCCTGCGGACTAAATTAAGCAATAAATTGATCGGTAAAAAACACAAAACGCTTatcatc                           >  2:720792/1‑149 (MQ=255)

                                                                                                                            aatGTCTGGCTTGTTCGCAGATTTCGTTAACCTTGTCAGGGGAGAAACAATGATTATTAATTAAATATTGCTCACAATTATCCACAAATTCCTGCGGACTAAATTCAGCAATAAATTGATCGGTAAAAAACACAAAACGCTTAtcatca                          <  1:1305579/149‑1 (MQ=255)

                                                                                                                             atGTCTGGCTTGTTCGCAGATTTAGTTAACCTTGTCAGGGGAGAAACAATGATTATTAATTAAATATTGCTCACAATTATCCACAAATTCCTGCGGACTAAATTCAGCAATAAATTGATCGGTAAAAAACACAAAACGCTTATcatcat                         <  1:4530578/149‑1 (MQ=255)

                                                                                                                              tGTCTGGCTTGTTCGCAGATTTCGTTAACCTTGTCAGGGGAGAAACAATGATTATTAATTAAATATTGCTCACAATTATCCACAAATTCCTGCGGACTAAATTCAGCAATAAATTGATCGGTAAAAAACACAAAACGCTTATCATCATg                        >  2:1356000/1‑149 (MQ=255)

                                                                                                                                tCTGGCTTGTTCGCAGATTTCGTTAACCTTGTCAGGGGAGAAACAATGATTATTAATTAAATATTGCTCACAATTATCCACAAATTCCTGCGGACTAAATTCAGCAATAAATTGATCGGTAAAAAACACAAAACGCTTATCATCATGaa                      >  2:2126263/1‑149 (MQ=255)

                                                                                                                                tCTGGCTTGTTCGCAGATTTCGTTAACCTTGTCAGGGGAGAAACAATGATTATTAATTAAATATTGCTCACAATTATCCACAAATTCCTGCGGACTAAATTCAGCAATAAATTGATCGGTAAAAAACACAAAACGCTTATCATCATGaa                      >  2:2126249/1‑149 (MQ=255)

                                                                                                                                tCTGGCTTGTTCGCAGATTTCGTTAACCTTGTCAGGGGAGAAACAATGATTATTAATTAAATATTGCTCACAATTATCCACAAATTCCTGCGGACTAAATTCAGCAATAAATTGATCGGTAAAAAACACAAAACGCTTATCATCATGaa                      <  2:5103350/149‑1 (MQ=255)

                                                                                                                                  tGGCTTGTTCGCAGATTTCGTTAACCTTGTCAGGGGAGAAACAATGATTATTAATTAAATATTGCTCACAATTATCCACAAATTCCTGCGGACTAAATTCAGCAATAAATTGATCGGTAAAAAACACAAAACGCTTATCATCATGAAAt                    >  2:2837726/1‑149 (MQ=255)

                                                                                                                                   ggCTTGTTCGCAGATTTCGTTAACCTTGTCAGGGGAGAAACAATGATTATTAATTAAATATTGCTCACAATTATCCACAAATTCCTGCGGACTAAATTCAGCAATAAATTGATCGGTAAAAAACACAAAACGCTTATCATCATGAAATg                   >  2:1841189/1‑149 (MQ=255)

                                                                                                                                   ggCTTGTTCGCAGATTTCGTTAACCTTGTCAGGGGAGAAACAATGATTATTAATTAAATATTGCTCACAATTATCCACAAATTCCTGCGGACTAAATTCAGCAATAAATTGATCGGTAAAAAACACAAAACGCTTATCATCATGAAATg                   >  1:4720120/1‑149 (MQ=255)

                                                                                                                                    gCTTGTTCGCAGATTTCGTTAACCTTGTCAGGGGAGAAACAATGATTATTAATTAAATATTGCTCACAATTATCCACAAATTCCTGCGGACTAAATTCAGCAATAAATTGATCGGTAAAAAACACAAAACGCTTATCATCATGAAATGt                  <  1:2619581/149‑1 (MQ=255)

                                                                                                                                      ttGTTCGCAGATTTAGTTAACCTTGTCAGGGGAGAAACAATGATTATTAATTAAATATTGCTCACAATTATCCACAAATTCCTGCGGACTAAATTCAGCAATAAATTGATCGGTAAAAAACACAAAACGCTTATCATCATGAAATGTaa                >  2:1926440/1‑149 (MQ=255)

                                                                                                                                       tGTTCGCAGATTTCGTTAACCTTGTCAGGGGAGAAACAATGATTATTAATTAAATATTGCTCACAATTATCCACAAATTCCTGCGGACTAAATTCAGCAATAAATTGATCGGTAAAAAACACAAAACGCTTATCATCATGAAATGTAat               <  2:2058604/149‑1 (MQ=255)

                                                                                                                                          tCGCAGATTTCGTTAACCTTGTCAGGGGAGAAACAATGATTATTAATTAAATATTGCTCACAATTATCCACAAATTCCTGCGGACTAAATTCAGCAATAAATTGATCGGTAAAAAACACAAAACGCTTATCATCATGAAATGTAATATg            <  1:4598749/149‑1 (MQ=255)

                                                                                                                                           cGCAGATTTCGTTAACCTTGTCAGGGGAGAAACAATGATTATTAATTAAATATTGCTCACAATTATCCACAAATTCCTGCGGACTAAATTCAGCAATAAATTGATCGGTAAAAAACACAAAACGCTTATCATCATGAAATGTAATATgg           >  1:234450/1‑149 (MQ=255)

                                                                                                                                             cAGATTTCGTTAACCTTGTCAGGGGAGAAACAATGATTATTAATTAAATATTGCTCACAATTATCCACAAATTCCTGCGGACTAAATTCAGCAATAAATTGATCGGTAAAAAACACAAAACGCTTATCATCATGAAATGTAATATGGAc         <  2:5117617/149‑1 (MQ=255)

                                                                                                                                              aGATTTCGTTAACCTTGTCAGGGGAGAAACAATGATTATTAATTAAATATTGCTCACAATTATCCACAAATTCCTGCGGACTAAATTCAGCAATAAATTGATCGGTAAAAAACACAAAACGCTTATCATCATGAAATGTAATATGGACa        >  2:3823023/1‑149 (MQ=255)

                                                                                                                                               gATTTAGTTAACCTTGTCAGGGGAGAAACAATGATTATTAATTAAATATTGCTCACAATTATCCACAAATTCCTGCGGACTAAATTCAGCAATAAATTGATCGGTAAAAAACACAAAACGCTTATCATCATGAAATGTAATATGGACAt       >  1:3300646/1‑149 (MQ=255)

                                                                                                                                                aTTTCGTTAACCTTGTCAGGGGAGAAACAATGATTATTAATTAAATATTGCTCACAATTATCCACAAATTCCTGCGGACTAAATTCAGCAATAAATTGATCGGTAAAAAACACAAAACGCTTATCATCATGAAGTGTAATATGGACAtt      >  2:880975/1‑149 (MQ=255)

                                                                                                                                                    cGTTAACCTTGTCAGGGGAGAAACAATGATTATTAATTAAATATTGCTCACAATTATCCACAAATTCCTGCGGACTAAATTCAGCAATAAATTGATCGGTAAAAAACACAAAACGCTTATCATCATGAAATGTAATATGGACATTCCat  >  1:3722895/1‑149 (MQ=255)

ATATTACAATTAGATTATATTCAAATCATTAAACTTGAGCCAGGGAGCTATAAGTCCTCAGGGCTTGCCAAAACAGAATTATTCATATCCAAAGAATGTGTTTCAAACATTTTTTCAACTAAATAATGTCTGGCTTGTTCGCAGATTTCGTTAACCTTGTCAGGGGAGAAACAATGATTATTAATTAAATATTGCTCACAATTATCCACAAATTCCTGCGGACTAAATTCAGCAATAAATTGATCGGTAAAAAACACAAAACGCTTATCATCATGAAATGTAATATGGACATTCCAT  >  CP009273/2988626‑2988922

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`