Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,989,295 C→A 93.1% pseudogene (85/261 nt) ygeN ← pseudogene

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,989,2950CA93.1% 616.8 / 34.8 189pseudogene (85/261 nt)ygeNpseudogene
Reads supporting (aligned to +/- strand):  ref base C (7/6);  new base A (92/84);  total (99/90)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.10e-01

Only 100 of 195 total aligned reads displayed.

GGGTATAAAATTATCCATCTCATCAATAATGCCTTTTGTATAGCCATCATAATAACCTTGAATAAATTTTTCTTCTCTCTTTTCCTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTCTTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCATCTCTATTTTTTTTCGCATAAAATGTAATTCTCTCTAAAAGTGTTGATGCAAGTGGTAACGGCGATTGTATGTGGTCAACA  >  CP009273/2989149‑2989441
                                                                                                                                                  |                                                                                                                                                  
 ggTATAAAATTATCCATCTCATCAATAATGCCTTTTGTATAGCCATCATAATAACCTTGAATAAATTTTTCTTCTCTCTTTTCCTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTatttattt                                                                                                                                                 <  2:3928153/149‑1 (MQ=255)
 ggTATAAAATTATCCATCTCATCAATAATGCCTTTTGTATAGCCATCATAATAACCTTGAATAAATTTTTCTTCTCTCTTTTCCTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTatttattt                                                                                                                                                 >  1:1082714/1‑149 (MQ=255)
     taAAATTATCCATCTCATCAATAATGCCTTTTGTATAGCCATCATAATAACCTTGAATAAATTTTTCTTCTCTCTTTTCCTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTCTTTCAAt                                                                                                                                             <  1:4528114/149‑1 (MQ=255)
      aaaaTTATCCATCTCATCAATAATGCCTTTTGTATAGCCATCATAATAACCTTGAATAAATTTTTCTTCTCTCTTTTCCTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTATTTCAATc                                                                                                                                            >  2:480285/1‑149 (MQ=255)
      aaaaTTATCCATCTCATCAATAATGCCTTTTGTATAGCCATCATAATAACCTTGAATAAATTTTTCTTCTCTCTTTTCCTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTATTTCAATc                                                                                                                                            >  2:478841/1‑149 (MQ=255)
      aaaaTTATCCATCTCATCAATAATGCCTTTTGTATAGCCATCATAATAACCTTGAATAAATTTTTCTTCTCTCTTTTCCTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTATTTCAATc                                                                                                                                            <  1:2099711/149‑1 (MQ=255)
         aTTATCCATATCTTCAATAATGCCTTTTGTATAGCCATCATAATAACCTTGAATAAATTTTTCTTCTCTCTTTTACTCAATACTCTTAATTTTTGATGTGGTTTCAAGAAGGATTTTTTATTTAAGAGAAACTATTTATTTAAAtaaat                                                                                                                                         >  2:3006881/1‑145 (MQ=255)
          ttATCCATCTCATCAATAATGCCTTTTGTATAGCCATCATAATAACCTTGAATAAATTTTTCTTCTCTCTTTTCCTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTCTTTCAATCCATc                                                                                                                                        >  2:951214/1‑149 (MQ=255)
           tATCCATCTCATCAATAATGCCTTTTGTATAGCCATCATAATAACCTTGAATAAATTTTTCTTCTCTCTTTTCCTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTATTTCAATCCATCa                                                                                                                                       >  1:4827894/1‑149 (MQ=255)
            aTCCATCTCATCAATAATGCCTTTTGTATAGCCATCATAATAACCTTGAATAAATTTTTCTTCTCTCTTTTCCTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTATTTCAATCCATCAg                                                                                                                                      >  1:485901/1‑149 (MQ=255)
              ccATCTCATCAATAATGCCTTTTGTATAGCCATCATAATAACCTTGAATAAATTTTTCTTCTCTCTTTTCCTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTATTTCAATCCATCAGaa                                                                                                                                    <  2:4105458/149‑1 (MQ=255)
               cATCTCATCAATAATGCCTTTTGTATAGCCATCATAATAACCTTGAATAAATTTTTCTTCTCTCTTTTCCTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTCTTTCAATCCATCAGaaa                                                                                                                                   <  1:4172646/149‑1 (MQ=255)
               cATCTCATCAATAATGCCTTTTGTATAGCCATCATAATAACCTTGAATAAATTTTTCTTCTCTCTTTTCCTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTATTTCAATCCATCAGaaa                                                                                                                                   <  1:5064162/149‑1 (MQ=255)
                 tctcATCAATAATGCCTTTTGTATAGCCATCATAATAACCTTGAATAAATTTTTCTTCTCTCTTTTCCTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTATTTCAATCCATCAGAAAtt                                                                                                                                 <  1:57342/149‑1 (MQ=255)
                 tctcATCAATAATGCCTTTTGTATAGCCATCATAATAACCTTGAATAAATTTTTCTTCTCTCTTTTCCTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTATTTCAATCCATCAGAAAtt                                                                                                                                 >  1:1726772/1‑149 (MQ=255)
                    catcaATAATGCCTTTTGTATAGCCATCATAATAACCTTGAATAAATTTTTCTTCTCTCTTTTCCTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTATTTCAATCCATCAGAAAttttt                                                                                                                              <  1:1193867/149‑1 (MQ=255)
                     atcaATAATGCCTTTTGTATAGCCATCATAATAACCTTGAATAAATTTTTCTTCTCTCTTTTCCTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTCTTTCAATCCATCAGAAATTTTTc                                                                                                                             >  2:2321798/1‑149 (MQ=255)
                      tcaATAATGCCTTTTGTATAGCCATCATAATAACCTTGAATAAATTTTTCTTCTCTCTTTTCCTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTATTTCAATCCATCAGAAATTTTTCt                                                                                                                            <  1:737254/149‑1 (MQ=255)
                      taaataatTCCTTTTTTATAGCCATCATAATAACCTTTAATAAATTTTTCTTCTCTCTTTTCCTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGTATTTTTTCTTTAAGAGAAACTATTTATTTCAATCCATCAGAAATTTTTCt                                                                                                                            <  1:2922390/147‑1 (MQ=255)
                       caATAATGCCTTTTGTATAGCCATCATAATAACCTTGAATAAATTTTTCTTCTCTCTTTTCCTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTCTTTCAATCCATCAGAAATTTTTCtt                                                                                                                           <  2:805054/149‑1 (MQ=255)
                        aataatGCCTTTTTTATAGCCATCATAATAACCTTGAATAAATTTTTCTTCTCTCTTTTCCTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTATTTCAATCCATCAGAAATTTTTCttt                                                                                                                          <  1:4918968/149‑1 (MQ=255)
                        aataatGCCTTTTGTATAGCCATCATAATAACCTTGAATAAATTTTTCTTCTCTCTTTTCCTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTATTTCAATCCATCAGAAATTTTTCttt                                                                                                                          <  1:622542/149‑1 (MQ=255)
                         ataatGCCTTTTGTATAGCCATCATAATAACCTTGAATAAATTTTTCTTCTCTCTTTTCCTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTATTTCAATCCATCAGAAATTTTTCtttt                                                                                                                         <  2:4542913/149‑1 (MQ=255)
                           aatGCCTTTTGTATAGCCATCATAATAACCTTGAATAAATTTTTCTTCTCTCTTTTCCTCAATACTCTGGATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTATTTCAATCCATCAGAAATTTTTCTTTTaa                                                                                                                       >  2:2570100/1‑149 (MQ=255)
                            atGCCTTTTGTATAGCCATCATAATAACCTTGAATAAATTTTTCTTCTCTCTTTTCCTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTATTTCAATCCATCAGAAATTTTTCTTTTAAt                                                                                                                      >  2:2407459/1‑149 (MQ=255)
                             tGCCTTTTGTATAGCCATCATAATAACCTTGAATAAATTTTTCTTCTCTCTTTTCCTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTATTTCAATCCATCAGAAATTTTTCTTTTAATc                                                                                                                     >  2:2921046/1‑149 (MQ=255)
                             tGCCTTTTGTATAGCCATCATAATAACCTTGAATAAATTTTTCTTCTCTCTTTTCCTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTATTTCAATCCATCAGAAATTTTTCTTTTAATc                                                                                                                     >  2:7755/1‑149 (MQ=255)
                               ccTTTTGTATAGCCATCATAATAACCTTGAATAAATTTTTCTTCTCTCTTTTCCTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTATTTCAATCCATCAGAAATTTTTCTTTTAATcac                                                                                                                   >  2:774326/1‑149 (MQ=255)
                                cTTTTGTATAGCCATCATAATAACCTTGAATAAATTTTTCTTCTCTCTTTTCCTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTATTTCAATCCATCAGAAATTTTTCTTTTAATcaca                                                                                                                  >  1:2369039/1‑149 (MQ=255)
                                       ataGCCATCATAATAACCTTGAATAAATTTTTCTTCTCTCTTTTCCTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTCTTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCAt                                                                                                           >  1:1092256/1‑149 (MQ=255)
                                       ataGCCATCATAATAACCTTGAATAAATTTTTCTTCTCTCTTTTCCTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTATTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCAt                                                                                                           >  1:2983790/1‑149 (MQ=255)
                                            catcatAATAACCTTGAATAAATTTTTCTTCTCTCTTTTCCTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTATTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCa                                                                                                      >  1:20195/1‑149 (MQ=255)
                                                 taataaCCTTGAATAAATTTTTCTTCTCTCTTTTCCTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTATTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGgaga                                                                                                 <  2:4711600/149‑1 (MQ=255)
                                                  aataaCCTTGAATAAATTTTTCTTCTCTCTTTTCCTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTATTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGAt                                                                                                <  1:957072/149‑1 (MQ=255)
                                                   ataaCCTTGAATAAATTTTTCTTCTCTCTTTTCCTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTATTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGAtt                                                                                               >  1:1825336/1‑149 (MQ=255)
                                                    taaCCTTGAATAAATTTTTCTTCTCTCTTTTCCTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTATTTCAATCCATCAGACATTTTTCTTTTAATCACAACACCAGTTGCAGGAGAttt                                                                                              >  1:2779806/1‑149 (MQ=255)
                                                    taaCATTGAATTAATTTTTCTTCTCTCTTTTCCTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTATTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGAttt                                                                                              <  2:4865624/149‑1 (MQ=255)
                                                     aaCCTTGAATAAATTTTTCTTCTCTCTTTTCCTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTATTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGTAGAtttt                                                                                             >  2:710449/1‑149 (MQ=255)
                                                     aaCCTTGAATAAATTTTTCTTCTCTCTTTTCCTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTATTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGAtttt                                                                                             <  1:614707/149‑1 (MQ=255)
                                                        cTTGAATAAATTTTTCTTCTCTCTTTTCCTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTATTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATc                                                                                          >  1:3759057/1‑149 (MQ=255)
                                                              tAAATTTTTCTTCTCTCTTTTCCTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTATTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTc                                                                                    >  2:1262662/1‑149 (MQ=255)
                                                                aaTTTTTCTTCTCTCTTTTCCTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTATTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCAt                                                                                  <  1:4563605/149‑1 (MQ=255)
                                                                  tttttCTTCTCTCTTTTCCTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTATTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCAtct                                                                                <  2:3122899/149‑1 (MQ=255)
                                                                   ttttCTTCTCTCTTTTCCTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTATTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCAtctc                                                                               <  1:1946499/149‑1 (MQ=255)
                                                                   ttttCTTCTCTCTTTTCCTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTATTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCAtctc                                                                               >  1:308881/1‑149 (MQ=255)
                                                                    tttCTTCTCTCTTTTCCTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTATTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAATCTCATctct                                                                              >  1:4094842/1‑149 (MQ=255)
                                                                     ttcttcTCTCTTTTCCTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTATTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCATCTCTa                                                                             >  2:4249152/1‑149 (MQ=255)
                                                                     ttcttcTCTCTTTTCCTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTATTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTAATAAAGCTCATCTCTa                                                                             >  2:4567444/1‑149 (MQ=255)
                                                                     ttcttcTCTATTTTCCTAAATACTCTGAATTTTTGCGGTGTTTTCAAGAAGGATTTTTTCTTTAAGAGAAAATATTTATTTCAATCCATCAGAAATTTTTCTTTTAATCACAAAAACATATTCAGTAGATTTTATAAAGATCATCtata                                                                             >  2:1632807/1‑149 (MQ=255)
                                                                      tcttctCTCTTTTCCTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTATTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCATCTCTAt                                                                            >  1:4796298/1‑149 (MQ=255)
                                                                      tcttctCTCTTTTCCTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTCTCTTTAAGAGAAACTATTTATTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCATCTCTAt                                                                            <  1:5036320/149‑1 (MQ=255)
                                                                       cttctCTCTTGTCCTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTATTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCATCTCTAtt                                                                           <  1:4640966/149‑1 (MQ=255)
                                                                          ctctctTTTCCTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTATTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCATCTCTAttttt                                                                        <  1:841163/149‑1 (MQ=255)
                                                                             tctTTTCCTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTATTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCATCTCTAtttttttt                                                                     >  1:4013158/1‑149 (MQ=255)
                                                                              ctTTTCCTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTATTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCATCTCTATTTTTTTTc                                                                    >  2:4171361/1‑149 (MQ=255)
                                                                               ttttCCTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTATTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCATCTCTATTTTTTTTCg                                                                   >  2:1993643/1‑149 (MQ=255)
                                                                                 ttCCTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTATTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCATCTCTAttttttttatca                                                                 >  1:3395818/1‑145 (MQ=255)
                                                                                  tCCTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTATTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCATCTCTATTTTTTTTCGCAt                                                                >  1:5218335/1‑149 (MQ=255)
                                                                                  tCCTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATATTTTATTTAAGAGAAACTATTTATTTCAATCCATCATAAATTTTTATTTTAATCACAAAAACATTTGCAGGAGATTTTATCAAGATCATCTCTATTTTTTTTAGCAt                                                                >  1:1527930/1‑149 (MQ=255)
                                                                                     tCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTATTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCATCTCTATTTTTTTTCGCATaaa                                                             <  2:2560806/149‑1 (MQ=255)
                                                                                     tCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTATTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCATCTCTA‑TTTTTTTCGCATaaaa                                                            >  2:1919131/1‑149 (MQ=255)
                                                                                          aCTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTATTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCATCTCTATTTTTTTTCGCATAAAATGTa                                                        >  1:3204303/1‑149 (MQ=255)
                                                                                            tctGAATTTTTGCGGTGGTTTCAAGAAGGATTTTCTCTTTAAGAGAAACTATTTATTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCATCTCTATTTTTTTTCGCATAAAATGTAAt                                                      <  1:5187548/149‑1 (MQ=255)
                                                                                              tGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTCTTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCATCTCTATTTTTTTTCGCATAAAATGTAATtc                                                    <  2:1183118/149‑1 (MQ=255)
                                                                                                aaTTTTTGCGGTGGTTTCAAGAAGTATTTTTTCTTTAAGAGAAACTATTTATTTCAATCCATCAGAAATTTTACATTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCATCTCTATTTTTTTTCGCAAAAAATGAAATtctc                                                  >  1:4059453/1‑149 (MQ=255)
                                                                                                aaTTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTATTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCATCTCTATTTTTTTTCGCATAAAATGTAATtctc                                                  <  1:2262043/149‑1 (MQ=255)
                                                                                                aaTTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTATTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCATCTCTATTTTTTTTCGCATAAAATGTAATtctc                                                  <  2:3398416/149‑1 (MQ=255)
                                                                                                        cGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTATTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCATCTCTATTTTTTTTCGCATAAAATGTAATTCTCTCTAAAAg                                          <  2:3900403/149‑1 (MQ=255)
                                                                                                         ggtggtTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTATTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCATCTCTATTTTTTTTCGCATAAAATGTAATTCTCTCTAAACgt                                         >  2:1696203/1‑149 (MQ=255)
                                                                                                           tggtTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTATTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCATCTCTATTTTTTTTCGCATAAAATGTAATTCTCTCTAAAAgtgt                                       <  2:2200107/149‑1 (MQ=255)
                                                                                                           tggtTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTATTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCATCTCTATTTTTTTTCGCATAAAATGTAATTCTCTCTAAAAgtgt                                       <  2:2426687/149‑1 (MQ=255)
                                                                                                             gtTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTATTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCATCTCTATTTTTTTTCGCATAAAATGTAATTCTCTCTAAAAGTGTTg                                     >  2:1754241/1‑149 (MQ=255)
                                                                                                             gtTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTATTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCATCTCTATTTTTTTTAGCATAAAATTTAATTCTCTCTAAAAGTGTTg                                     >  2:1550062/1‑149 (MQ=255)
                                                                                                               ttCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTCTTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCATCTCTATTTTTTTTCGCATAAAATGTAATTCTCTCTAAAAGTGTTGAt                                   >  1:2333523/1‑149 (MQ=255)
                                                                                                               ttCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTATTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCATCTCTATTTTTTTTCGCATAAAATGTAATTCTCTCTAAAAGTGTTGAt                                   <  2:1475544/149‑1 (MQ=255)
                                                                                                               ttCAAGAAGGATTTTTTATTTAAGAGAAACTATTTATTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCATCTCTATTTTTTTTCGCATAAAATGTAATTCTCTCTAAAAGTGTTGAt                                   <  2:3579818/149‑1 (MQ=255)
                                                                                                                  aagaagGATTTTTTCTTTAAGAGAAACTATTTATTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCATCTCTATTTTTTTTCGCATAAAATGTAATTCTCTCTAAAAGTGTTGATGCa                                <  1:4711175/149‑1 (MQ=255)
                                                                                                                  aagaagGATTTTTTCTTTAAGAGAAACTATTTATTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCATCTCTATTTTTTTTCGCATAAAATGTAATTCTCTCTAAAAGTGTTGATGCa                                <  1:2608069/149‑1 (MQ=255)
                                                                                                                   agaagGATTTTTTCTTTAAGAGAAACTATTTATTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCATCTCTATTTTTTTTCGCATAAAATGTAATTCTCTCTAAAAGTGTTGATGCaa                               >  2:568945/1‑149 (MQ=255)
                                                                                                                   agaagGATTTTTTCTTTAAGAGAAACTATTTATTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCATCTCTATTTTTTTTCGCATAAAATGTAATTCTCTCTAAAAGTGTTGATGCaa                               >  1:2968541/1‑149 (MQ=255)
                                                                                                                    taatgATTTTTTATTTAAGAGAAACTATTTATTTCAATAAATCAGAAATTTTTCTTTTAATCACAACAACATTTGCATGATATTTTATCAATCTCATCTCTATTTTTTTTAGCATAAAATGTAATTCTCTCTAAAAGTGTTGATGCAAg                              <  2:3680644/145‑1 (MQ=255)
                                                                                                                     aagGATTTTTTCTTTAAGAGAAACTATTTATTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCATCTCTATTTTTTTTCGCATAAAATGTAATTCTCTCTAAAAGTGTTGATGCAAGt                             >  1:4404527/1‑149 (MQ=255)
                                                                                                                     aagGATTTTTTCTTTAAGAGAAACTATTTATTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCATCTCTATTTTTTTTCGCATAAAATGTAATTCTCTCTAAAAGTGTTGATGCAAGt                             >  1:3654192/1‑149 (MQ=255)
                                                                                                                     aagGATTTTTTCTTTAAGAGAAACTATTTATTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCATCTCTATTTTTTTTCGCATAAAATGTAATTCTCTCTAAAAGTGTTGATGCAAGt                             <  2:1082714/149‑1 (MQ=255)
                                                                                                                       ggATTTTTTCTTTAAGAGAAACTATTTATTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCATCTCTATTTTTTTTCGCATAAAATGTAATTCTCTCTAAAAGTGTTGATGCAAGTgg                           >  1:2866862/1‑149 (MQ=255)
                                                                                                                          ttttttCTTTAAGAGAAACTATTTATTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCATCTCTATTTTTTTTCGCATAAAATGTAATTCTCTCTAAAAGTGTTGATGCAAGTGGTaa                        >  1:1334354/1‑149 (MQ=255)
                                                                                                                          ttttttCTTTAAGAGAAACTATTTATTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCATCTCTATTTTTTTTCGCATAAAATGTAATTCTCTCTAAAAGTGTTGATGCAAGTGGTaa                        >  1:1882832/1‑149 (MQ=255)
                                                                                                                          ttttttCTTTAAGAGAAACTATTTATTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCATCTCTATTTTTTTTCGCATAAAATGTAATTCTCTCTAAAAGTGTTGATGCAAGTGGTaa                        <  2:1320961/149‑1 (MQ=255)
                                                                                                                               tCTTTAAGAGAAACTATTTATTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCATCTCTATTTTTTTTCGCATAAAATGTAATTCTCTCTAAAAGTGTTGATGCAAGTGGTAACGGCg                   <  1:570057/149‑1 (MQ=255)
                                                                                                                                  ttAAGAGAAACTATTTCTTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCATCTCTATTTTTTTTCGCATAAAATGTAATTCTCTCTAAAAGTGTTGATGCAAGTGGTAACGGCGAtt                <  1:2536698/149‑1 (MQ=255)
                                                                                                                                    aaGAGAAACTATTTATTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCATCTCTATTTTTTTTCGCATAAAATGTAATTCTCTCTAAAAGTGTTGATGCAAGTGGTAACGGCGATTGt              <  2:3654192/149‑1 (MQ=255)
                                                                                                                                    aaGAGAAACTATTTATTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCATCTCTATTTTTTTTCGCATAAAATGTAATTCTCTCTAAAAGTGTTGATGCAAGTGGTAACGGCGATTGt              >  1:2239008/1‑149 (MQ=255)
                                                                                                                                        gaAACTATTTATTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCATCTCTATTTTTTTTCGCATAAAATGTAATTCTCTCTAAAAGTGTTGATGCAAGTGGTAACGGCGATTGTAtgt          >  2:3216658/1‑149 (MQ=255)
                                                                                                                                        gaAACTATTTATTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCATCTCTATTTTTTTTCGCATAAAATGTAATTCTCTCTAAAAGTGTTGATGCAAGTGGTAACGGCGATTGTAtgt          <  1:1262662/149‑1 (MQ=255)
                                                                                                                                        gaAACTATTTATTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCATCTCTATTTTTTTTCGCATAAAATGTAATTCTCTCTAAAAGTGTTGATGCAAGTGGTAACGGCGATTGTAtgt          >  2:2808669/1‑149 (MQ=255)
                                                                                                                                         aaaCTATTTATTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAATCTCATCTCTATTTTTTTTCGCATAAAATGTAATTCTCCCTAAAAGTGTTGATGCAAGTGGTAACGGCGATTGTAtgtg         <  2:2349010/149‑1 (MQ=255)
                                                                                                                                           aCTATTTCTTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCATCTCTATTTTTTTTCGCATAAAATGTAATTCTCTCTAAAAGTGTTGATGCAAGTGGTAACGGCGATTGTATGTGGt       >  1:5227012/1‑149 (MQ=255)
                                                                                                                                           aCTATTTATTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCATCTCTATTTTTTTTCGCATAAAATGTAATTCTCTCTAAAAGTGTTGATGCAAGTGGTAACGGCGATTGTATGTGGt       <  2:5006230/149‑1 (MQ=255)
                                                                                                                                               tttatttcAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCATCTCTATTTTTTTTCGCATAAAATGTAATTCTCTCTAAAAGTGTTGATGCAAGTGGTAACGGCGATTGTATGTGGTcaac   <  2:1726772/149‑1 (MQ=255)
                                                                                                                                                ttctttcAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCATCTCTATTTTTTTTCGCATAAAATGTAATTCTCTCTAAAAGTGTTGATGCAAGTGGTAACGGCGATTGTATGTGGTcaaca  >  2:3350287/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
GGGTATAAAATTATCCATCTCATCAATAATGCCTTTTGTATAGCCATCATAATAACCTTGAATAAATTTTTCTTCTCTCTTTTCCTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTCTTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCATCTCTATTTTTTTTCGCATAAAATGTAATTCTCTCTAAAAGTGTTGATGCAAGTGGTAACGGCGATTGTATGTGGTCAACA  >  CP009273/2989149‑2989441

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑