Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,993,211 C→A 91.7% V14L (GTG→TTG)  ygeR ← LysM domain‑containing M23 family putative peptidase; septation lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,993,2110CA91.7% 956.7 / 72.5 301V14L (GTG→TTG) ygeRLysM domain‑containing M23 family putative peptidase; septation lipoprotein
Reads supporting (aligned to +/- strand):  ref base C (13/12);  new base A (124/152);  total (137/164)
Fisher's exact test for biased strand distribution p-value = 5.34e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.63e-01

Only 100 of 301 total aligned reads displayed.

TTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCACGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGGAAAAACGTGGTAATAGAGAAAGAGGTTAATTTC  >  CP009273/2993065‑2993358
                                                                                                                                                  |                                                                                                                                                   
ttttACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGa                                                                                                                                                   <  1:1470841/149‑1 (MQ=255)
ttttACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGa                                                                                                                                                   >  1:5116535/1‑149 (MQ=255)
    aCGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATAcc                                                                                                                                               >  1:2148579/1‑149 (MQ=255)
      gCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCa                                                                                                                                             <  2:3984050/149‑1 (MQ=255)
        tGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCaga                                                                                                                                           <  2:2698773/149‑1 (MQ=255)
        tGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCaga                                                                                                                                           >  1:195467/1‑149 (MQ=255)
         ggTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCagag                                                                                                                                          >  1:1007394/1‑149 (MQ=255)
         ggTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCagag                                                                                                                                          >  2:749459/1‑149 (MQ=255)
          gTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAgaga                                                                                                                                         <  2:4801623/149‑1 (MQ=255)
             cccGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGAttt                                                                                                                                      <  1:58007/149‑1 (MQ=255)
               cGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGAttttt                                                                                                                                    >  1:1687839/1‑149 (MQ=255)
               cGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGAttttt                                                                                                                                    >  2:1497731/1‑149 (MQ=255)
                gtggtgCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGAtttttt                                                                                                                                   <  1:3454804/149‑1 (MQ=255)
                 tggtgCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTa                                                                                                                                  <  1:5235493/149‑1 (MQ=255)
                    tgCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCACGATACCCAGAGATTTTTTATTc                                                                                                                               >  2:3643312/1‑149 (MQ=255)
                     gcgcGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCa                                                                                                                              <  1:4002958/149‑1 (MQ=255)
                       gcgAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAgg                                                                                                                            <  2:2107144/149‑1 (MQ=255)
                        cgAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGc                                                                                                                           <  1:2070556/149‑1 (MQ=255)
                         gAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCg                                                                                                                          >  2:865001/1‑149 (MQ=255)
                          aaaTACGATATAGCGTATCCCCCCGTTTCACGGTGTAGACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACGTCAAGATACCCAGAGATTTTTTATTCAGGCGt                                                                                                                         <  2:3820638/149‑1 (MQ=255)
                          aaaTACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGt                                                                                                                         <  2:258061/149‑1 (MQ=255)
                            aTACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTcc                                                                                                                       <  2:1086186/149‑1 (MQ=255)
                             tACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTccc                                                                                                                      >  1:3715741/1‑149 (MQ=255)
                             tACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTccc                                                                                                                      >  2:135976/1‑149 (MQ=255)
                              aCGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCg                                                                                                                     >  1:1550359/1‑149 (MQ=255)
                                  tataGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACt                                                                                                                 >  1:807723/1‑149 (MQ=255)
                                       cGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCaaaa                                                                                                            <  1:1527348/149‑1 (MQ=255)
                                        gTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAAt                                                                                                           >  1:3059773/1‑149 (MQ=255)
                                        gTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAAt                                                                                                           <  1:2692279/149‑1 (MQ=255)
                                         tATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATc                                                                                                          >  1:249941/1‑149 (MQ=255)
                                             cccccGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTc                                                                                                      >  1:4896204/1‑149 (MQ=255)
                                              ccccGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTcc                                                                                                     >  2:1054372/1‑149 (MQ=255)
                                               cccGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCt                                                                                                    >  2:407578/1‑149 (MQ=255)
                                                ccGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTc                                                                                                   <  1:4513761/149‑1 (MQ=255)
                                                  gTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGc                                                                                                 >  2:2460985/1‑149 (MQ=255)
                                                          gtgtAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTaaaaaa                                                                                         >  2:691140/1‑149 (MQ=255)
                                                             tAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACaa                                                                                      >  1:5074034/1‑149 (MQ=255)
                                                               atcGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGa                                                                                    <  2:3318908/147‑1 (MQ=255)
                                                               aaCGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGa                                                                                    >  1:4976767/1‑149 (MQ=255)
                                                                   gAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGcc                                                                                >  1:2894980/1‑149 (MQ=255)
                                                                    aGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCt                                                                               <  2:4329876/149‑1 (MQ=255)
                                                                      ccGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTa                                                                             <  2:3165362/149‑1 (MQ=255)
                                                                       cGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTAt                                                                            <  1:2618229/149‑1 (MQ=255)
                                                                       cGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTC‑AAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATc                                                                           <  1:2452978/149‑1 (MQ=255)
                                                                         gAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCACGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCa                                                                          <  1:2325459/149‑1 (MQ=255)
                                                                         gAATACGGTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCa                                                                          <  2:585856/149‑1 (MQ=255)
                                                                         gAAGACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCa                                                                          <  2:2491486/149‑1 (MQ=255)
                                                                          aaTACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCACGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCAt                                                                         <  1:4173249/149‑1 (MQ=255)
                                                                              cGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATagca                                                                     <  1:3585998/149‑1 (MQ=255)
                                                                                ttCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATagcagc                                                                   >  2:290835/1‑149 (MQ=255)
                                                                                     gTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCagccagcc                                                              <  1:4205706/149‑1 (MQ=255)
                                                                                      tATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGACAGCct                                                             >  1:3046429/1‑149 (MQ=255)
                                                                                       aTCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCctc                                                            <  2:3721036/149‑1 (MQ=255)
                                                                                        tCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCctct                                                           <  1:3217227/149‑1 (MQ=255)
                                                                                         cGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCtt                                                          <  2:1405185/149‑1 (MQ=255)
                                                                                           gatgatTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGa                                                        <  2:1776625/149‑1 (MQ=255)
                                                                                            atgatTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGAt                                                       <  1:4661579/149‑1 (MQ=255)
                                                                                            atgatTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGAt                                                       <  1:954171/149‑1 (MQ=255)
                                                                                             tgatTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATa                                                      <  1:4413865/149‑1 (MQ=255)
                                                                                                 ttGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTCAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCa                                                  >  1:3676603/1‑149 (MQ=255)
                                                                                                  tGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCaa                                                 <  2:2208213/149‑1 (MQ=255)
                                                                                                  tGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCaa                                                 <  1:3612064/149‑1 (MQ=255)
                                                                                                      aCCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAAtctc                                             <  1:2996919/149‑1 (MQ=255)
                                                                                                       cccGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATctct                                            <  2:5116535/149‑1 (MQ=255)
                                                                                                        ccGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCtt                                           <  2:2273346/149‑1 (MQ=255)
                                                                                                          gAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGc                                         >  1:3166760/1‑149 (MQ=255)
                                                                                                          gAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGc                                         >  1:2449000/1‑149 (MQ=255)
                                                                                                           aaCAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCt                                        >  2:660159/1‑149 (MQ=255)
                                                                                                           aaCAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCt                                        <  2:4356126/149‑1 (MQ=255)
                                                                                                            aCAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCACTCTCGTGCtt                                       >  2:2792053/1‑149 (MQ=255)
                                                                                                             cAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTc                                      >  2:2142009/1‑149 (MQ=255)
                                                                                                               gCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTg                                    >  2:4670653/1‑149 (MQ=255)
                                                                                                               gCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTg                                    >  2:617927/1‑149 (MQ=255)
                                                                                                               gCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTg                                    >  2:3766432/1‑149 (MQ=255)
                                                                                                                 ccGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGGa                                  >  2:3025449/1‑149 (MQ=255)
                                                                                                                     cAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGGaaaaa                              <  2:4597936/149‑1 (MQ=255)
                                                                                                                     cAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGGaaaaa                              >  1:4061097/1‑149 (MQ=255)
                                                                                                                       aaaGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGGAAAAACg                            <  2:4002889/149‑1 (MQ=255)
                                                                                                                        aaGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGGAAAAACGt                           <  1:1994145/149‑1 (MQ=255)
                                                                                                                         aGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGGAAAAACGTg                          <  2:5185859/149‑1 (MQ=255)
                                                                                                                         aGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGGAAAAACGTg                          <  2:3607040/149‑1 (MQ=255)
                                                                                                                                cAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGGAAAAACGTGGTAATag                   <  2:4722736/149‑1 (MQ=255)
                                                                                                                                cAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGGAAAAACGTGGTAATag                   <  2:409188/149‑1 (MQ=255)
                                                                                                                                 aaCCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGGAAAAACGTGGTAATaga                  <  2:2034389/149‑1 (MQ=255)
                                                                                                                                   ccGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGGAAAAACGTGGTAATAgaga                <  2:1889139/149‑1 (MQ=255)
                                                                                                                                    cGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGGAAAAACGTGGTAATAGAGaa               <  1:1881438/149‑1 (MQ=255)
                                                                                                                                    cGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGGAAAAACGTGGTAATAGAGaa               <  2:3261375/149‑1 (MQ=255)
                                                                                                                                    cGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGGAAAAACGTGGTAATAGAGaa               <  1:3882766/149‑1 (MQ=255)
                                                                                                                                     gATAACAACATCACGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGGAAAAACGTGGTAATAGAGaaa              >  1:274964/1‑149 (MQ=255)
                                                                                                                                     gATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGGAAAAACGTGGTAATAGAGaaa              <  2:3123749/149‑1 (MQ=255)
                                                                                                                                      aTAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGGAAAAACGTGGTAATAGAGAAag             <  1:482278/149‑1 (MQ=255)
                                                                                                                                      aTAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGGAAAAACGTGGTAATAGAGAAag             >  2:1300536/1‑149 (MQ=255)
                                                                                                                                       tAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGGAAAAACGTGGTAATAGAGAAaga            <  1:1155861/149‑1 (MQ=255)
                                                                                                                                        aacaacATCACGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGGAAAAACGTGGTAATAGAGAAagag           >  1:3324076/1‑149 (MQ=255)
                                                                                                                                         acaacaTCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGGAAAAACGTGGTAATAGAGAAAGAgg          >  1:1788333/1‑149 (MQ=255)
                                                                                                                                         acaacaTCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGGAAAAACGTGGTAATAGAGAAAGAgg          <  1:1247672/149‑1 (MQ=255)
                                                                                                                                          caacaTCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGGAAAAACGTGGTAATAGAGAAAGAGGt         <  2:3470357/149‑1 (MQ=255)
                                                                                                                                           aacaTCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGGAAAAACGTGGTAATAGAGAAAGAGGtt        <  2:195467/149‑1 (MQ=255)
                                                                                                                                            acaTCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGGAAAAACGTGGTAATAGAGAAAGAGGTTa       <  2:114470/149‑1 (MQ=255)
                                                                                                                                                 aCGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGGAAAAACGTGGTAATAGAGAAAGAGGTTAATTTc  <  1:4131138/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
TTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCACGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGGAAAAACGTGGTAATAGAGAAAGAGGTTAATTTC  >  CP009273/2993065‑2993358

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑