Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,080,759 T→G 8.9% V232G (GTT→GGT)  metK → S‑adenosylmethionine synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,080,7590TG 8.9% 569.3 / 7.9 233V232G (GTT→GGT) metKS‑adenosylmethionine synthetase
Reads supporting (aligned to +/- strand):  ref base T (84/122);  new base G (25/2);  total (109/124)
Fisher's exact test for biased strand distribution p-value = 1.51e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 244 total aligned reads displayed.

CTGTCGTGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCGGTGCATTCTCTGGTAAAGATCCATCAAAAGTGGACCGTTCCGCAGCCTACGCAGCACGTTAT  >  CP009273/3080612‑3080907
                                                                                                                                                   |                                                                                                                                                    
 tGTCGAGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTa                                                                                                                                                    <  1:989782/149‑1 (MQ=255)
  gTCGAGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTAt                                                                                                                                                   >  1:2799279/1‑149 (MQ=255)
  gTCGAGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTAt                                                                                                                                                   <  2:2197171/149‑1 (MQ=255)
  gTCGAGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTGTGGTTAt                                                                                                                                                   >  1:1930658/1‑149 (MQ=255)
   tCGAGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATc                                                                                                                                                  <  1:4414874/149‑1 (MQ=255)
      agCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTGTCGGTATAggg                                                                                                                                               >  1:506347/2‑148 (MQ=255)
           tCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGccc                                                                                                                                          <  1:912656/149‑1 (MQ=255)
           tCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGccc                                                                                                                                          <  1:913432/149‑1 (MQ=255)
              aCTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAAt                                                                                                                                       <  2:670355/149‑1 (MQ=255)
               cTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATg                                                                                                                                      <  1:1559674/149‑1 (MQ=255)
                tCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACACGACCGGTCGTTTCGTTATCGGTGGCCCAAttg                                                                                                                                     <  1:1456424/149‑3 (MQ=255)
                 catcACTCTGAAGATATCTACCATAAATCGCTGCAAGAATATTTAATTGAAGAGATCATCAATCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATggg                                                                                                                                    <  1:2871362/146‑1 (MQ=255)
                 cAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCCATggg                                                                                                                                    >  1:1668027/1‑149 (MQ=255)
                  aGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTGTCGTTATAGGTGGCCCAAAGGGt                                                                                                                                   >  1:3233515/1‑149 (MQ=255)
                  aGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTGTCGGTATCGGTGGGCCAATGGGt                                                                                                                                   >  1:3711111/1‑149 (MQ=255)
                   gCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTGTCGTTATCGGTGGCCCAAAGGGTg                                                                                                                                  >  2:648166/1‑149 (MQ=255)
                         tGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGCCTGCg                                                                                                                            >  2:3747702/1‑149 (MQ=255)
                           aaGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGt                                                                                                                          <  2:819368/149‑1 (MQ=255)
                              agaTCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTg                                                                                                                       <  2:4062732/149‑1 (MQ=255)
                                aTCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTgtc                                                                                                                     >  2:2004709/1‑147 (MQ=255)
                                aTCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTGTGGGTATCGGGGGCCCCAAGGGTGTCTGCGGTCTGAc                                                                                                                     >  1:1005240/1‑149 (MQ=255)
                                  cGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGACTg                                                                                                                   <  1:305566/149‑1 (MQ=255)
                                       aGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGTGTGGCTGGTCGt                                                                                                              >  2:4591116/1‑149 (MQ=255)
                                        gAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGTCGTa                                                                                                             >  2:4476268/1‑149 (MQ=255)
                                        gAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGTCGTa                                                                                                             <  1:700138/149‑1 (MQ=255)
                                        gAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGTCGTa                                                                                                             <  1:2538417/149‑1 (MQ=255)
                                         aaaTCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATAGGTGGCCCCAAGGGTGACTGCGGTCTGACTGGTGGTaa                                                                                                            >  1:317963/1‑149 (MQ=255)
                                            tCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAAt                                                                                                         <  2:3258751/149‑1 (MQ=255)
                                             cGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAAtt                                                                                                        <  1:4015914/149‑1 (MQ=255)
                                               cTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTAt                                                                                                      <  1:3347305/149‑1 (MQ=255)
                                                tGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTGTCGTTATAGGTGGCCCAATTGGTGACTGCGGTCTGGCTGGTCGTAAAATTAta                                                                                                     >  1:293871/1‑148 (MQ=255)
                                                  cAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGt                                                                                                   <  1:4167322/149‑1 (MQ=255)
                                                    agaagCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTg                                                                                                 <  1:713932/149‑1 (MQ=255)
                                                    agaagCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTGTCGGTATCGGGGGCCCCAAGGGTGTCTGCGGTCTGACGGGGCGTAAAATTATCGTTg                                                                                                 >  2:2833/1‑149 (MQ=255)
                                                     gaagCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGGTTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGa                                                                                                <  2:3406502/149‑1 (MQ=255)
                                                       agCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATa                                                                                              <  2:3831467/149‑1 (MQ=255)
                                                           gTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTGTGGTTATCGGGGGCCCAAAGGGTGACTGCGGGCTGACTGGTCGTAAAATTATAGTTGATAccaa                                                                                          >  1:5163317/1‑147 (MQ=255)
                                                            tAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTAc                                                                                         <  1:1234026/149‑1 (MQ=255)
                                                            tAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTAc                                                                                         >  2:4502600/1‑149 (MQ=255)
                                                            tAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTAc                                                                                         <  1:543677/149‑1 (MQ=255)
                                                            tAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTAc                                                                                         <  1:543620/149‑1 (MQ=255)
                                                            tAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTAc                                                                                         <  2:4932901/149‑1 (MQ=255)
                                                              aTGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGGTTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTAcgg                                                                                       <  2:1456785/149‑1 (MQ=255)
                                                                  aaGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACggcggc                                                                                   <  1:75971/149‑1 (MQ=255)
                                                                  aaGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACggcggc                                                                                   <  2:308037/149‑1 (MQ=255)
                                                                   agagATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCTAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCa                                                                                  <  2:303514/149‑1 (MQ=255)
                                                                          atcaAGCCGATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTGTCGGTATCGGTGGCCCCATGGGTGACTGCGGTCTGACTGGTCGGAAAATTATAGTTGATACCAACGGCGGCATGGcgcg                                                                           >  2:5120334/1‑149 (MQ=255)
                                                                          atcaAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGGTTCGTTATCGGTGGCCCAAAGGGTGACTGCGGTCGGACTGGTCGGAAAATTATAGTTGATACCTACGGCGGCATGGcgcg                                                                           >  1:3455945/1‑149 (MQ=255)
                                                                           tcaAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTGTCGGTATAGGTGGCCCCAAGGGTGACGGCGGGCTGGCGGGGCGTAAAATTATAGTTGATAACAACGGCGGCATGGCGCGt                                                                          >  1:3930881/1‑149 (MQ=255)
                                                                               gCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTGTGGTTATAGGGGGGCCAAAGGGTGTCTGCGGGGTGACAGGTAGTAAAATTATAGTTGTTAACAAAGGCGGCCAGGCGCGTCACg                                                                      >  1:1142795/1‑149 (MQ=255)
                                                                                ccAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACgg                                                                     <  2:3938887/149‑1 (MQ=255)
                                                                                ccAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACgg                                                                     <  2:1227285/149‑1 (MQ=255)
                                                                                    ttCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGc                                                                 <  2:3039420/149‑1 (MQ=255)
                                                                                    ttCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGGTTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGc                                                                 <  2:4217896/149‑1 (MQ=255)
                                                                                    gtCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGc                                                                 <  2:3551315/148‑1 (MQ=255)
                                                                                     tCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCg                                                                <  2:1075665/149‑1 (MQ=255)
                                                                                     tCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAAATATAGTTGATAACTACGGCGGCATGGCGCGTCACGGTGGCg                                                                >  2:5185954/1‑149 (MQ=255)
                                                                                      cTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCgg                                                               <  1:1816915/149‑1 (MQ=255)
                                                                                      cTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCgg                                                               <  1:429475/149‑1 (MQ=255)
                                                                                       tGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTGTGGGTATGGGTGGGCCCAAGGGTGTCTGCGGTCTGAATTGTGGTAAAAATATATTTGATAACAAAAGCGGCAATGCGCGTCACCGGGGCGGt                                                              >  1:1780229/1‑149 (MQ=255)
                                                                                       tGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTGTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGACGGGTCGTAAAATTATCGGTGATACCTACGGCGGCATGGCGCGGCACGGTGGCggg                                                              >  2:4100755/1‑148 (MQ=255)
                                                                                        gCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCGGTg                                                             <  1:2110818/149‑1 (MQ=255)
                                                                                         cccGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCGGTGc                                                            <  2:1045888/149‑1 (MQ=255)
                                                                                          ccGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCGGTGCa                                                           >  2:796487/1‑149 (MQ=255)
                                                                                          ccGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCGGTGCa                                                           <  1:2412625/149‑1 (MQ=255)
                                                                                            gCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCGGTGCAtt                                                         <  2:4408711/149‑1 (MQ=255)
                                                                                             cTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGTCTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCGGTGCATtc                                                        >  2:1646842/1‑149 (MQ=255)
                                                                                             cTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCGGTGCATtc                                                        <  1:2665898/149‑1 (MQ=255)
                                                                                               gAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCGGTGCATtctc                                                      <  1:4502600/149‑1 (MQ=255)
                                                                                               gAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCGGTGCATtctc                                                      <  2:3030191/149‑1 (MQ=255)
                                                                                               gAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCGGTGCATtctc                                                      <  2:1349666/149‑1 (MQ=255)
                                                                                               gAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTGTCGTTATCGGTGGCCCCATGGGTGACGGCGGGCGGACTGGTGGTAAAATTATCGTTGATAACTACGGCGGCATGGCGCGTCACCGGGGCGGTGGATtctc                                                      >  2:5029880/1‑149 (MQ=255)
                                                                                                aaTGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCGGTGCATTctct                                                     <  1:1027826/149‑1 (MQ=255)
                                                                                                  tGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCGGTGCATTCTCTgg                                                   <  1:2756794/149‑1 (MQ=255)
                                                                                                    gCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTGGGTATCGGTGGCCCAAAGGGGGTCAGCGGTCTGACTGGTCGTAAAAATATAGTTGATAACAACGGCGGCCAGGCGCGTCACCGTGGCGGTGCATTCTCTgggc                                                 >  1:1210302/1‑147 (MQ=255)
                                                                                                    gCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTGTCGGTATCGGTGGCCCCAAGGGTGACGGCGGGCGGACGGGGCCTAAAAATATCGGTGATACCAAACGCCGCCAGGCGCGGCACCGTGGCGGTGCCTTCGCTGGtc                                                 >  1:3329537/1‑148 (MQ=255)
                                                                                                      tGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCGGTGCATTCTCTGGTaaa                                               <  1:1355338/149‑1 (MQ=255)
                                                                                                       gACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGTAGTAAAAATATAGTTGATACCAACGGCGGCATGGCGCGTCACGGTGGCGGTGCATTCTCTGGTaaat                                              >  1:4761223/1‑148 (MQ=255)
                                                                                                            cTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCGGTGCATTCTCTGGTAAAGATCCa                                         <  2:1569904/149‑1 (MQ=255)
                                                                                                              gCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATAGGTGGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCGGTGCATTCTCTGGTAAAGATCCATc                                       >  2:4676642/1‑149 (MQ=255)
                                                                                                                caccaAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCGGTGCATTCTCTGGTAAAGATCCATCaa                                     <  1:2966907/149‑1 (MQ=255)
                                                                                                                 accaAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCGGTGCATTCTCTGGTAAAGATCCATCaaa                                    <  2:1541080/149‑1 (MQ=255)
                                                                                                                 accaAATTCTTCATCAACCCGACCGGTCGTGTCGTTATAGGTGGCCCAAATGGTGTCTGCGGTCTGACAGGTGGTAAAAATATAGTTGATACCAAAGGCGGCATGGCGCGTCACGGTGGCGGTGCATTGTCTGGGAAAGATACAACaaa                                    >  1:2085724/1‑149 (MQ=255)
                                                                                                                   caAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCGGTGCATTCTCTGGTAAAGATCCATCAAAAg                                  <  2:28883/149‑1 (MQ=255)
                                                                                                                     aaTTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAAATATCGTTGATACCTACGGCGGCAAGGCGCGTCACGGTGGCGGTGCAATCTCTGGTAAAGATCCATCAAAAGTg                                >  2:4143037/1‑149 (MQ=255)
                                                                                                                     aaTTCTTCATCAACCCGACCGGTCGTTTCGTTATAGGTGGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTTATAACTACGGCGGCATGGCGCGTCACGGTGGCGGTGCATTCTCTGGTAAATATCAATAAAAAGtt                                >  2:1984761/1‑148 (MQ=255)
                                                                                                                        tcttcATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCGGTGCATTCTCTGGTAAAGATCCATCAAAAGTGGAc                             <  1:4382722/149‑1 (MQ=255)
                                                                                                                        tcttcATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCGGTGCATTCTCTGGTAAAGATCCATCAAAAGTGGAc                             <  1:4566835/149‑1 (MQ=255)
                                                                                                                         cttcATCAACCCGACCGGTCGTTTCGGTATCGGTGGCCCAAAGGGTGACTGCGGGATGACGTGGAGTAAAATTATCGTTTATAACAAAGGCGGCAATGCGCGTCAACGGGGCGGTGCAAACTCTGGTAAAGATACAACAAAAGTGGaac                            >  2:2282639/1‑147 (MQ=255)
                                                                                                                            catcaACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCGGTGCATTCTCTGGTAAAGATCCATCAAAAGTGGACCGtt                         <  1:2694749/149‑1 (MQ=255)
                                                                                                                            catcaACCCGACCGGTCGTTTCGTTATAGGTGGCCCCATGGGTGACTGCGGTCTGACGGGTCGTAAAATTATAGTTGATACCTACGGCGGCATGGCGCGGCACGGTGGCGGTGCATTATCTGGGAAAGATCCAACCAAAGTGGACCGtt                         >  2:4451767/1‑149 (MQ=255)
                                                                                                                              tcaACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCGGTGCATTCTCTGGTAAAGATCCATCAAAAGTGGACCGTTcc                       <  2:1111753/149‑1 (MQ=255)
                                                                                                                               caACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGGTGATACCTACGGCGGCATGGCGCGTCACGGTGGCGGTGCATTCTCTGGTAAAGATCCAACAAAAGTGGACCGTTCCg                      >  2:331584/1‑149 (MQ=255)
                                                                                                                                aaCCCGACCGGTCGTTTCGGTATAGGTGGCCCAAAGGGTGACTGCGGTCTGACTGGTCGTAAAATTATAGTTGATAACTACCGCGGCAAGGCGCGTCACGGTGGCGGTGCATTATATGGTAAAGATCCATCCAAAGTGGACCGTTACGc                     >  2:2686312/1‑149 (MQ=255)
                                                                                                                                 aCCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCGGTGCATTCTCTGGTAAAGATCCATCAAAAGTGGACCGTTCCGCa                    <  2:2555706/149‑1 (MQ=255)
                                                                                                                                  cccGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGTCTGGTCGTAAAATTATCGTTGATACCAACGGCGGCATTGCGCGTCACGGTGGCGGTGCATTCTATGGTAAAGATCCATCAAAAGTGGACCGTTCCGCAg                   >  2:1238800/1‑149 (MQ=255)
                                                                                                                                   ccGACCGGTCGTGTCGTTATCGGTGGCCCAAAGGGTGACGGCGGTCTGACTGGTCGTGAAAATATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCGGTGCATTCTCTGGTAAAGATCCATCAAAAGTGGACCGTTCCGCAGc                  >  2:5198988/1‑149 (MQ=255)
                                                                                                                                    cGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCGGTGCATTCTCTGGTAAAGATCCATCAAAAGTGGACCGTTCCGCAGcc                 <  2:1907541/149‑1 (MQ=255)
                                                                                                                                    cGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGTAGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCGGTGCATTCTCTGGTAAAGATCCATCAAAAGTGGACCGTTACGCAGcc                 >  2:3463980/1‑149 (MQ=255)
                                                                                                                                          gTCGTTTCGGTATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCGGTGCATTCTCGGGTAAAGATCCATCAAAAGTGGACCGTTCCGCAGCCTACgca           <  1:1571863/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
CTGTCGTGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCGGTGCATTCTCTGGTAAAGATCCATCAAAAGTGGACCGTTCCGCAGCCTACGCAGCACGTTAT  >  CP009273/3080612‑3080907

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑