Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,202,506 C→A 5.7% S375* (TCG→TAG)  ttdT → L‑tartrate/succinate antiporte

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,202,5060CA 5.7% 1253.1 / 35.7 328S375* (TCG→TAG) ttdTL‑tartrate/succinate antiporte
Reads supporting (aligned to +/- strand):  ref base C (188/120);  new base A (14/6);  total (202/126)
Fisher's exact test for biased strand distribution p-value = 4.85e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 331 total aligned reads displayed.

ATCAGTTGGGACGACATTGTCAGTAATAAAGCGGCGTGGAACGTTTTCTTCTGGCTGGCCTCGCTTATCACCCTCGCCACCGGACTCAACAACACCGGTTTTATTAGCTGGTTTGGCAAACTGTTAGCAGGCAGCTTAAGCGGTTATTCGCCAACGATGGTGATGGTGGCGTTGATTGTGGTGTTTTATCTACTGCGCTACTTTTTCGCCAGCGCCACGGCGTATACCTCCGCTCTCGCACCGATGATGATTGCTGCTGCGCTGGCGATGCCGGAAATCCCGCTGCCGGTGTTCT  >  CP009273/3202358‑3202652
                                                                                                                                                    |                                                                                                                                                  
     ttGGGACGACATTGTCAGTAATAAAGCGGCGTGGAACGTTTTCTTCTGGCTGGCCTCGCTTATCACCCTCGCCACCGGACTCAACAACACCGGTTTTATTAGCTGGTTTGGCAAACTGTTAGCAGGCAGCTTAAGCGGTTATTCGCCaa                                                                                                                                               >  2:5075824/1‑149 (MQ=255)
       gggACGACATTGTCAGTAATAAAGCGGCGTGGAACGTTTTCTTCTGGCTGGCCTCGCTTATCACCCTCGCCACCGGACTCAACAACACCGGGTTTATTAGCTGGTTTGGCAAACTGTTAGCAGGCAGCGTACGCGGTTATTCGCCAACg                                                                                                                                             >  2:4840380/1‑149 (MQ=255)
       gggACGACATTGTCAGTAATAAAGCGGCGTGGAACGTTTTCTTCAGGCTGGCCTCGCTTATCACCCTCGCCACCGGACTCAACAACACCGGATTTATTAGCTGGTTTGGCAAACTGTTAGCAGGCAGCTTAAGCGGTTATTCGCCAACg                                                                                                                                             >  1:5130686/1‑149 (MQ=255)
        ggACGACATTGTCAGTAATAAAGCGGCGTGGAACGTTTTCTTCTGGCTGGCCTCGCTTATCACCCTCGCCACCGGACTCAACAACACCGGTTTTATTAGCTGGTTTGGCAAACTGTTAGCAGGCAGCTTAAGCGGTTATTAGCCAACga                                                                                                                                            <  1:2056058/149‑1 (MQ=255)
         gacgacATTGTCAGTAATAAAGCGGCGTGGAACGTTTTCTTCTGGCTGGCCTCGCTTATCACCCTCGCCACCGGACTCAACAACACCGGTTTTATTAGCTGGTTTGGCAAACTGTTAGCAGGCAGCTTAAGCGGTTATTCGCCAACgat                                                                                                                                           <  1:3543978/149‑1 (MQ=255)
          acgacATTGTCAGTAATAAAGCGGCGTGGAACGTTTTCTTCTGGCTGGCCTCGCTTATCACCCTCGCCACCGGACTCAACAACACCGGTTTTATTAGCTGGTTTGGCAAACTGTTAGCAGGCAGCTTAAGCGGTTATTCGCCAACgatg                                                                                                                                          >  2:1451570/1‑149 (MQ=255)
           cgacATTGTCAGTAATAAAGCGGCGTGGAACGTTTTCTTCTGGCTGGCCTCGCTTATCACCCTCGCCACCGGACTCAACAACACCGGTTTTATTAGCTGGTTTGGCAAACTGTTAGCAGGCAGCTTAAGCGGTTATTCGCCAACgatgg                                                                                                                                         <  2:4025756/149‑1 (MQ=255)
            gacATTGTCAGTAATAAAGCGGCGTGGAACGTTTTCTTCTGGCTGGCCTCGCTTATCACCCTCGCCACCGGACTCAACAACACCGGTTTTATTAGCTGGTTTGGCAAACTGTTAGCAGGCAGCTTAAGCGGTTATTCGCCAACgatggt                                                                                                                                        >  1:3876123/1‑149 (MQ=255)
             acATTGTCAGTAATAAAGCGGCGTGGAACGTTTTCTTCTGGCTGGCCTCGCTTATCACCCTCGCCACCGGACTCAACAACACCGGTTTTATTAGCTGGTTTGGCAAACTGTTAGCAGGCAGCTTAAGCGGTTATTCGCCAACgatggtg                                                                                                                                       >  1:1148865/1‑149 (MQ=255)
             acATTGTCAGTAATAAAGCGGCGTGGAACGTTTTCTTCTGGCTGGCCTCGCTTATCACCCTCGCCACCGGACTCAACAACACCGGTTTTATTAGCTGGTTTGGCAAACTGTTAGCAGGCAGCTTAAGCGGTTATTCGCCAACgatggtg                                                                                                                                       >  1:4570379/1‑149 (MQ=255)
              cATTGTCAGTAATAAAGCGGCGTGGAACGTTTTCTTCTGGCTGGCCTCGCTTATCACCCTCGCCACCGGACTCAACAACACCGGTTTTATTAGCTGGTTTGGCAAACTGTTAGCAGGCAGCTTAAGCGGTTATTCGCCAACgatggtga                                                                                                                                      >  1:1803400/1‑149 (MQ=255)
               aTTGTCAGTAATAAAGCGGCGTGGAACGTTTTCTTCTGGCTGGCCTCGCTTATCACCCTCGCCACCGGACTCAACAACACCGGTTTTATTAGCTGGTTTGGCAAACTGTTAGCAGGCAGCTTAAGCGGTTATTCGCCAACgatggtgat                                                                                                                                     >  1:4947099/1‑149 (MQ=255)
                   tCAGTAATAAAGCGGCGTGGAACGTTTTCTTCTGGCTGGCCTCGCTTATCACCCTCGCCACCGGACTCAACAACACCGGTTTTATTAGCTGGTTTGGCAAACTGTTAGCAGGCAGCTTAAGCGGTTATTCGCCAACGatggtgatggtg                                                                                                                                 >  2:100586/1‑149 (MQ=255)
                   tCAGTAATAAAGCGGCGTGGAACGTTTTCTTCTGGCTGGCCTCGCTTATCACCCTCGCCACCGGACTCAACAACACCGGTTTTATTAGCTGGTTTGGCAAACTGTTAGCAGGCAGCTTAAGCGGTTATTCGCCAACGagggtgatggtg                                                                                                                                 >  2:2656014/1‑149 (MQ=255)
                   tCAGTAATAAAGCGGCGTGGAACGTTTTCTTCTGGCTGGCCTCGCTGATCACCCTCGCCACCGGACTCAACAACACCGGTTTTATTAGCTGGTTTGGCAAACTGTTAGCAGGCAGCTTAAGCGGTTATTCGCCAACGatggtgatggtg                                                                                                                                 <  2:3903781/149‑1 (MQ=255)
                     aGTAATAAAGCGGCGTGGAACGTTTTCTTCTGGCTGGCCTCGCTTATCACCCTCGCCACCGGACTCAACAACACCGGTTTTATTAGCTGGTTTGGCAAACTGTTAGCAGGCAGCTTAAGCGGTTATTCGCCAACGATGGTGATGGTGGc                                                                                                                               >  2:249258/1‑149 (MQ=255)
                       taataaAGCGGCGTGGAACGTTTTCTTCTGGCTGGCCTCGCTTATCACCCTCGCCACCGGACTCAACAACACCGGTTTTATTAGCTGGTTTGGCAAACTGTTAGCAGGCAGCTTAAGCGGTTATTCGCCAACGATGGTGATGGTGGCGt                                                                                                                             <  1:2464384/149‑1 (MQ=255)
                       taataaAGCGGCGTGGAACGTTTTCTTCTGGCTGGCCTCGCTTATCACCCTCGCCACCGGACTCAACAACACCGGTTTTATTAGCTGGTTTGGCAAACTGTTAGCAGGCAGCTTAAGCGGTTATTCGCCAACGATGGTGATGGTGGCGt                                                                                                                             >  1:2768293/1‑149 (MQ=255)
                        aataaAGCGGCGTGGAACGTTTTCTTCTGGCTGGCCTCGCTTATCACCCTCGCCACCGGACTCAACAACACCGGTTTTATTAGCTGGTTTGGCAAACTGTTAGCAGGCAGCTTAAGCGGTTATTCGCCAACGATGGTGATGGTGGCGtt                                                                                                                            <  1:5036394/149‑1 (MQ=255)
                        aataaAGCGGCGTGGAACGTTTTCTTCTGGCTGGCCTCGCTTATCACCCTCGCCACCGGACTCAACAACACCGGTTTTATTAGCTGGTTTGGCAAACTGTTAGCAGGCAGCTTAAGCGGTTATTCGCCAACGATGGTGATGGTGGCGtt                                                                                                                            <  2:2829037/149‑1 (MQ=255)
                         ataaAGCGGCGTGGAACGTTTTCTTCTGGCTGGCCTCGCTTATCACCCTCGCCACCGGACTCAACAACACCGGTTTTATTAGCTGGTTTGGCAAACTGTTAGCAGGCAGCTTAAGCGGTTATTCGCCAACGATGGTGATGGTGGCGTTg                                                                                                                           <  1:1331855/149‑1 (MQ=255)
                         ataaAGCGGCGTGGAACGTTTTCTTCTGGCTGGCCTCGCTTATCACCCTCGCCACCGGACTCAACAACACCGGTTTTATTAGCTGGTTTGGCAAACTGTTAGCAGGCAGCTTAAGCGGTTATTCGCCAACGATGGTGATGGTGGCGTTg                                                                                                                           >  1:4784541/1‑149 (MQ=255)
                         ataaAGCGGCGTGGAACGTTTTCTTCTGGCTGGCCTCGCTTATCACCCTCGCCACCGGACTCAACAACACCGGTTTTATTAGCTGGTTTGGCAAACTGTTAGCAGGCAGCTTAAGCGGTTATTAGCCAACGATGGTGATGGTGGCGTTg                                                                                                                           >  1:1988783/1‑149 (MQ=255)
                           acaGCGGCGTGGAACGATTTCTTCTGGCTGGCCTCGCTTATCACCCTCGCCACCTGACTCAACAACACCGGTTTTATTAGCTGGTTTGGCAAACTGTTAGCAGGCAGCTTAAGCGGTTATTCGCCAACGATGGTGATGGTTGCGTTGAt                                                                                                                         <  2:540954/147‑1 (MQ=255)
                           aaaGCGGCGTGGAACGTTTTCTTCTGGCTGGCCTCGCTTATCACCCTCGCCACCGGACTCAACAACACCGGTTTTATTAGCTGGTTTGGCAAACTGTTAGCAGGCAGCTTAAGCGGTTATTCGCCAACGATGGTGATGGTGGCGTTGAt                                                                                                                         >  2:320027/1‑149 (MQ=255)
                           aaaGCGGCGTGGAACGTTTTCTTCTGGCTGGCCTCGCTTATCACCCTCGCCACCGGACTCAACAACACCGGTTTTATTAGCTGGTTTGGCAAACTGTTAGCAGGCAGCTTAAGCGGTTATTCGCCAACGATGGTGATGGTGGCGTTGAt                                                                                                                         >  1:2889938/1‑149 (MQ=255)
                            aaGCGGCGTGGAACGTTTTCTTCTGGCTGGCCTCGCTTATCACCCTCGCCACCGGACTCAACAACACCGGTTTTATTAGCTGGTTTGGCAAACTGTTAGCAGGCAGCTTAAGCGGTTATTCGCCAACGATGGTGATGGTGGCGTTGAtt                                                                                                                        <  1:1955800/149‑1 (MQ=255)
                               cggcgTGGAACGTTTTCTTCTGGCTGGCCTCGCTTATCACCCTCGCCACCGGACTCAACAACACCGGTTTTATTAGCTGGTTTGGCAAACTGTTAGCAGGCAGCTTAAGCGGTTATTCGCCAACGATGGTGATGGTGGCGTTGATtgtg                                                                                                                     <  2:3047652/149‑1 (MQ=255)
                                 gcgTGGAACGTTTTCTTCTGGCTGGCCTCGCTTATCACCCTCGCCACCGGACTCAACAACACCGGTTTTATTAGCTGGTTTGGCAAACTGTTAGCAGGCAGCTTAAGCGGTTATTCGCCAACGATGGTGATGGTGGCGTTGATTgtggt                                                                                                                   >  2:196634/1‑149 (MQ=255)
                                 gcgTGGAACGTTTTCTTCTGGCTGGCCTCGCTTATCACCCTCGCCACCGGACTCAACAACACCGGTTTTATTAGCTGGTTTGGCAAACTGTTAGCAGGCAGCTTAAGCGGTTATTCGCCAACGATGGTGATGGTGGCGTTGATTgtggt                                                                                                                   <  1:2592569/149‑1 (MQ=255)
                                 gcgTGGAACGTTTTCTTCTGGCTGGCCTCGCTTATCACCCTCGCCACCGGACTCAACAACACCGGTTATATTAGCTGGTTAGGCAAACTGTAAGCAGGCAGCTTAAGCGGTTCTTCGCCAACGATGGTGATGGTGGCGTTGATTgtggt                                                                                                                   >  2:5250730/1‑149 (MQ=255)
                                   gTGGAACGTTTTCTTCTGGCTGGCCTCGCTTATCACCCTCGCCACCGGACTCAACAACACCGGTTTTATTAGCTGGTTTGGCAAACTGTTAGCAGGCAGCTTAAGCGGTTATTCGCCAACGATGGTGATGGTGGCGTTGATTGTGgtgt                                                                                                                 >  2:5190164/1‑149 (MQ=255)
                                     ggAACGTTTTCTTCTGGCTGGCCTCGCTTATCACCCTCGCCACCGGACTCAACAACACCGGTTTTATTAGCTGGTTTGGCAAACTGTTAGCAGGCAGCTTAAGCGGTTATTCGCCAACGATGGTGATGGTGGCGTTGATTGTGGTGttt                                                                                                               <  2:2024147/149‑1 (MQ=255)
                                      gAACGTTTTCTTCTGGCTGGCCTCGCTTATCACCCTCGCCACCGGACTCAACAACACCGGTTTTATTAGCTGGTTTGGCAAACTGTTAGCAGGCAGCTTAAGCGGTTATTCGCCAACGATGGTGATGGTGGCGTTGATTGTGGTGtttt                                                                                                              <  1:382445/149‑1 (MQ=255)
                                      gAACGTTTTCTTCTGGCTGGCCTCGCTTATCACCCTCGCCACCGGACTCAACAACACCGGTTTTATTAGCTGGTTTGGCAAACTGTTAGCAGGCAGCTTAAGCGGTTATTCGCCAACGATGGTGATGGTGGCGTTGATTGTGGTGtttt                                                                                                              <  1:4048069/149‑1 (MQ=255)
                                       aaCGTTTTCTTCTGGCTGGCCTCGCTTATCACCCTCGCCACCGGACTCAACAACACCGGTTTTATTAGCTGGTTTGGCAAACTGTTAGCAGGCAGCTTAAGCGGTTATTCGCCAACGATGGTGATGGTGGCGTTGATTGTGGTGTTTTa                                                                                                             >  2:4362553/1‑149 (MQ=255)
                                       aaCGTTTTCTTCTGGCTGGCCTCGCTTATCACCCTCGCCACCGGACTCAACAACACCGGTTTTATTAGCTGGTTTGGCAAACTGTTAGCAGGCAGCTTAAGCGGTTATTCGCCAACGATGGTGATGGTGGCGTTGATTGTGGTGTTTTa                                                                                                             <  1:3053598/149‑1 (MQ=255)
                                       aaCGTTTTCTTCTGGCTGGCCTCGCTTATCACCCTCGCCACCGGACTCAACAACACCGGTTTTATTAGCTGGTTTGGCAAACTGTTAGCAGGCAGCTTAAGCGGTTATTAGCCAACGATGGTGATGGTGGCGTTGATTGTGGTGTTTTa                                                                                                             >  2:1306804/1‑149 (MQ=255)
                                        aCGTTTTCTTCTGGCTGGCCTCGCTTATCACCCTCGCCACCGGACTCAACAACACCGGTTTTATTAGCTGGTTTGGCAAACTGTTAGCAGGCAGCTTAAGCGGTTATTCGCCAACGATGGTGATGGTGGCGTTGATTGTGGTGTTTTAt                                                                                                            >  2:1149041/1‑149 (MQ=255)
                                         cGTTTTCTTCTGGCTGGCCTCGCTTATCACCCTCGCCACCGGACTCAACAACACCGGTTTTATTAGCTGGTTTGGCAAACTGTTAGCAGGCAGCTTAAGCGGTTATTCGCCAACGATGGTGATGGTGGCGTTGATTGTGGTGTTTTATc                                                                                                           <  2:183809/149‑1 (MQ=255)
                                         cGTTTTCTTCTGGCTGGCCTCGCTTATCACCCTCGCCACCGGACTCAACAACACCGGTTTTATTAGCTGGTTTGGCAAACTGTTAGCAGGCAGCTTAAGCGGTTATTCGCCAACGATGGTGATGGTGGCGTTGATTGTGGTGTTTTATc                                                                                                           >  1:716616/1‑149 (MQ=255)
                                           ttttCTTCTGGCTGGCCTCGCTTATCACCCTCGCCACCGGACTCAACAACACCGGTTTTATTAGCTGGTTTGGCAAACTGTTAGCAGGCAGCTTAAGCGGTTATTCGCCAACGATGGTGATGGTGGCGTTGATTGTGGTGTTTTATCTa                                                                                                         >  1:746629/1‑149 (MQ=255)
                                            tttCTTCTGGCTGGCCTCGCTTATCACCCTCGCCACCGGACTCAACAACACCGGTTTTATTAGCTGGTTTGGCAAACTGTTAGCAGGCAGCTTAAGCGGTTATTCGCCAACGATGGTGATGGTGGCGTTGATTGTGGTGTTTTATCTAc                                                                                                        >  2:2182896/1‑149 (MQ=255)
                                            tttCTTCTGGCTGGCCTCGCTTATCACCCTCGCCACCGGACTCAACAACACCGGTTTTATTAGCTGGTTTGGCAAACTGTTAGCAGGCAGCTTAAGCGGTTATTAGCCAACGATGGTGATGGTGGCGTTGATTGTGGTGTTTTATCTAc                                                                                                        >  2:3275648/1‑149 (MQ=255)
                                             ttcttcTGGCTGGCCTCGCTTATCACCCTCGCCACCGGACTCAACAACACCGGTTTTATTAGCTGGTTTGGCAAACTGTTAGCAGGCAGCTTAAGCGGTTATTCGCCAACGATGGTGATGGTGGCGTTGATTGTGGTGTTTTATCTACt                                                                                                       >  2:3752723/1‑149 (MQ=255)
                                                     gctggcCTCGCTTATCACCCTCGCCACCGGACTCAACAACACCGGTTTTATTAGCTGGTTTGGCAAACTGTTAGCAGGCAGCTTAAGCGGTTATTCGCCAACGATGGTGATGGTGGCGTTGATTGTGGTGTTTTATCTACTGCGCTACt                                                                                               <  2:4003951/149‑1 (MQ=255)
                                                         gcCTCGCTTATCACCCTCGCCACCGGACTCAACAACACCGGTTTTATTAGCTGGTTTGGCAAACTGTTAGCAGGCAGCTTAAGCGGTTATTCGCCAACGATGGTGATGGTGGCGTTGATTGTGGTGTTTTATCTACTGCGCTACttttt                                                                                           <  2:423204/149‑1 (MQ=255)
                                                          ccTCGCTTATCACCCTCGCCACCGGACTCAACAACACCGGTTTTATTAGCTGGTTTGGCAAACTGTTAGCAGGCAGCTTAAGCGGTTATTCGCCAACGATGGTGATGGTGGCGTTGATTGTGGTGTTTTATCTACTGCGCTACTTTTTc                                                                                          >  2:2338663/1‑149 (MQ=255)
                                                                 aataaCCCTCGCCACCGGACTCAACAACACCGGTTTTATTAGCTGGTTTGGCAAACTGTTAGCAGGCAGCTTAAGCGGTTATTCGCCAACGAGTGTGATGGTGGCGTTGATTGTGGTGTTTTATCTACTGCGCTACTTTTTCGCCAgcg                                                                                   >  2:2531834/5‑149 (MQ=255)
                                                                    cACCCTCGCCACCGGACTCAACAACACCGGTTTTATTAGCTGGTTTGGCAAACTGTTAGCAGGCAGCTTAAGCGGTTATTCGCCAACGATGGTGATGGTGGCGTTGATTGTGGTGTTTTATCTACTGCGCTACTTTTTCGCCAGCGCCa                                                                                >  1:695423/1‑149 (MQ=255)
                                                                     aCCCTCGCCACCGGACTCAACAACACCGGTTTTATTAGCTGGTTTGGCAAACTGTTAGCAGGCAGCTTAAGCGGTTATTCGCCAACGATGGTGATGGTGGCGTTGATTGTGGTGTTTTATCTACTGCGCTACTTTTTCGCCAGCGCCAc                                                                               >  1:140472/1‑149 (MQ=255)
                                                                     aCCCTCGCCACCGGACTCAACAACACCGGTTTTATTAGCTGGTTTGGCAAACTGTTAGCAGGCAGCTTAAGCGGTTATTCGCCAACGATGGTGATGGTGGCGTTGATTGTGGTGTTTTATCTACTGCGCTACTTTTTCGCCAGCGCCAc                                                                               <  2:36683/149‑1 (MQ=255)
                                                                     aCCCTCGCCACCGGACTCAACAACACCGGTTTTATTAGCTGGTTTGGCAAACTGTTAGCAGGCAGCTTAAGCGGTTATTCGCCAACGATGGTGATGGTGGCGTTGATTGTGGTGTTTTATCTACTGCGCTACTTTTTCGCCAGCGCCAc                                                                               <  1:34164/149‑1 (MQ=255)
                                                                     aCCCTCGCCACCGGACTCAACAACACCGGTTTTATTAGCTGGTTTGGCAAACTGTTAGCAGGCAGCTTAAGCGGTTATTCGCCAACGATGGTGATGGTGGCGTTGATTGTGGTGTTTTATCTACTGCGCTACTTTTTCGCCAGCGCCAc                                                                               <  1:32412/149‑1 (MQ=255)
                                                                       ccTCGCCACCGGACTCAACAACACCGGTTTTATTAGCTGGTTTGGCAAACTGTTAGCAGGCAGCTTAAGCGGTTATTCGCCAACGATGGTGATGGTGGCGTTGATTGCGGTGTTTTATCTACTGCGCTACTTTTTCGCCAGCGCCACgg                                                                             <  2:4479793/149‑1 (MQ=255)
                                                                        cTCGCCACCGGACTCAACAACACCGGTTTTATTAGCTGGTTTGGCAAACTGTTAGCAGGCAGCTTAAGCGGTTATTCGCCAACGATGGTGATGGTGGCGTTGATTGTGGTGTTTTATCTACTGCGCTACTTTTTCGCCAGCGCCACGGc                                                                            <  2:4564612/149‑1 (MQ=255)
                                                                           gCCACCGGACTCAACAACACCGGTTTTATTAGCTGGTTTGGCAAACTGTTAGCAGGCAGCTTAAGCGGTTATTCGCCAACGATGGTGATGGTGGCGTTGATTGTGGTGTTTTATCTACTGCGCTACTTTTTCGCCAGCGCCACGGCGta                                                                         <  1:2187600/149‑1 (MQ=255)
                                                                           gCCACCGGACTCAACAACACCGGTTTTATTAGCTGGTTTGGCAAACTGTTAGCAGGCAGCTTAAGCGGTTATTCGCCAACGATGGTGATGGTGGCGTTGATTGTGGTGTTTTATCTACTGCGCTACTTTTTCGCCAGCGCCACGGCGta                                                                         <  1:3132820/149‑1 (MQ=255)
                                                                             cACCGGACTCAACAACACCGGTTTTATTAGCTGGTTTGGCAAACTGTTAGCAGGCAGCTTAAGCGGTTATTCGCCAACGATGGTGATGGTGGCGTTGATTGTGGTGTTTTATCTACTGCGCTACTTTTTCGCCAGCGCCACGGCGtata                                                                       >  2:125333/1‑149 (MQ=255)
                                                                                 ggACTCAACAACACCGGTTTTATTAGCTGGTTTGGCAAACTGTTAGCAGGCAGCTTAAGCGGTTATTCGCCAACGATGGTGATGGTGGCGTTGATTGTGGTGTTTTATCTACTGCGCTACTTTTTCGCCAGCGCCACGGCGTATACCTc                                                                   >  1:854110/1‑149 (MQ=255)
                                                                                 ggACTCAACAACACCGGTTTTATTAGCTGGTTTGGCAAACTGTTAGCAGGCAGCTTAAGCGGTTATTCGCCAACGATGGTGATGGTGGCGTTGATTGTGGTGTTTTATCTACTGCGCTACTTTTTCGCCAGCGCCACGGCGTATACCTc                                                                   >  1:3480559/1‑149 (MQ=255)
                                                                                     tCAACAACACCGGTTTTATTAGCTGGTTTGGCAAACTGTTAGCAGGCAGCTTAAGCGGTTATTCGCCAACGATGGTGATGGTGGCGTTGATTGTGGTGTTTTATCTACTGCGCTACTTTTTCGCCAGCGCCACGGCGTATACCTCCGct                                                               <  1:4118016/149‑1 (MQ=255)
                                                                                     tCAACAACACCGGTTTTATTAGCTGGTTTGGCAAACTGTTAGCAGGCAGCTTAAGCGGTTATTCGCCAACGATGGTGATGGTGGCGTTGATTGTGGTGTTTTATCTACTGCGCTACTTTTTCGCCAGCGCCACGGCGTATACCTCCGct                                                               <  1:3340009/149‑1 (MQ=255)
                                                                                          aacaCCGGTTTTATTAGCTGGTTTGGCAAACTGTTAGCAGGCAGCTTAAGCGGTTATTCGCCAACGATGGTGATGGTGGCGTTGATTGTGGTGTTTTATCTACTGCGCTACTTTTTCGCCAGCGCCACGGCGTATACCTCCGCTCTCGc                                                          >  1:1670747/1‑149 (MQ=255)
                                                                                           acacCGGTTTTATTAGCTGGTTTGGCAAACTGTTAGCAGGCAGCTTAAGCGGTTATTCGCCAACGATGGTGATGGTGGCGTTGATTGTGGTGTTTTATCTACTGCGCTACTTTTTCGCCAGCGCCACGGCGTATACCTCCGCTCTCGCa                                                         >  1:428279/1‑149 (MQ=255)
                                                                                               cGGTTTTATTAGCTGGTTTGGCAAACTGTTAGCAGGCAGCTTAAGCGGTTATTCGCCAACGATGGTGATGGTGGCGTTGATTGTGGTGTTTTATCTACTGCGCTACTTTTTCGCCAGCGCCACGGCGTATACCTCCGCTCTCGCACCga                                                     <  1:1706377/149‑1 (MQ=255)
                                                                                                 gTTTTATTAGCTGGTTTGGCAAACTGTTAGCAGGCAGCTTAAGCGGTTATTCGCCAACGATGGTGATGGTGGCGTTGATTGTGGTGTTTTATCTACTGCGCTACTTTTTCGCCAGCGCCACGGCGTATACCTCCGCTCTCGCACCgatg                                                   >  2:3841047/1‑149 (MQ=255)
                                                                                                  ttttATTAGCTGGTTTGGCAAACTGTTAGCAGGCAGCTTAAGCGGTTATTCGCCAACGATGGTGATGGTGGCGTTGATTGTGGTGTTTTATCTACTGCGCTACTTTTTCGCCAGCGCCACGGCGTATACCTCCGCTCTCGCACCgatga                                                  >  1:4409501/1‑149 (MQ=255)
                                                                                                   tttATTAGCTGGTTTGGCAAACTGTTAGCAGGCAGCTTAAGCGGTTATTCGCCAACGATGGTGATGGTGGCGTTGATTGTGGTGTTTTATCTACTGCGCTACTTTTTCGCCAGCGCCACGGCGTATACCTCCGCTCTCGCACCgatgat                                                 <  2:1093037/149‑1 (MQ=255)
                                                                                                     tattaGCTGGTTTGGCAAACTGTTAGCAGGCAGCTTAAGCGGTTATTAGCCAACGATGGTGATGGTGGCGTTGATTGTGGTGTTTTATCTACTGCGCTACTTTTTCGCCAGCGCCACGGCGTATACCTCCGCTCTCGCACCgatgatga                                               >  2:4264038/1‑149 (MQ=255)
                                                                                                     tatgaGCTGGTTTGGCAAACTGTTAGCAGGCAGCTTAAGCGGTTATTCGCCAACGATGGTGATGGTGGCGTTGATTGTGGTGTTTTATCTACTGCGCTACTTTTTCGCCAGCGCCACGGCGTATACCTCCGCTCTCGCACCgatgatga                                               <  2:2784080/149‑1 (MQ=255)
                                                                                                      attaGCTGGTTTGGCAAACTGTTAGCAGGCAGCTTAAGCGGTTATTCGCCAACGATGGTGATGGTGGCGTTGATTGTGGTGTTTTATCTACTTCGCTACTTTTTCGCCAGCGCCACGGCGTATACCTCCGCTCTCGCACCgatgatgat                                              >  2:3372350/1‑149 (MQ=255)
                                                                                                      attaGCTGGTTTGGCAAACTGTTAGCAGGCAGCTTAAGCGGTTATTCGCCAACGATGGTGATGGTGGCGTTGATTGTGGTGTTTTATCTACTGCGCTACTTTTTCGCCAGCGCCACGGCGTATACCTCCGCTCTCGCACCgatgatgat                                              >  1:4736416/1‑149 (MQ=255)
                                                                                                      attaGCTGGTTTGGCAAACTGTTAGCAGGCAGCTTAAGCGGTTATTCGCCAACGATGGTGATGGTGGCGTTGATTGTGGTGTTTTATCTACTGCGCTACTTTTTCGCCAGCGCCACGGCGTATACCTCCGCTCTCGCACCgaggatgat                                              >  2:891396/1‑149 (MQ=255)
                                                                                                        taGCTGGTTTGGCAAACTGTTAGCAGGCAGCTTAAGCGGTTATTCGCCAACGATGGTGATGGTGGCGTTGATTGTGGTGTTTTATCTACTGCGCTACTTTTTCGCCAGCGCCACGGCGTATACCTCCGCTCTCGCACCGATGATGATtg                                            <  1:3767339/149‑1 (MQ=255)
                                                                                                         aGCTGGTTTGGCAAACTGTTAGCAGGCAGCTTAAGCGGTTATTCGCCAACGATGGTGATGGTGGCGTTGATTGTGGTGTTTTATCTACTGCGCTACTTTTTCGCCAGCGCCACGGCGTATACCTCCGCTCTCGCACCGATGATGATtgc                                           <  1:1152100/149‑1 (MQ=255)
                                                                                                            tGGTTTGGCAAACTGTTAGCAGGCAGCTTAAGCGGTTATTCGCCAACGATGGTGATGGTGGCGTTGATTGTGGTGTTTTATCTACTGCGCTACTTTTTCGCCAGCGCCACGGCGTATACCTCCGCTCTCGCACCGATGATGATtgctgc                                        >  1:3061734/1‑149 (MQ=255)
                                                                                                                 tGGCAAACTGTTAGCAGGCAGCTTAAGCGGTTATTCGCCAACGATGGTGATGGTGGCGTTGATTGTGGTGTTTTATCTACTGCGCTACTTTTTCGCCAGCGCCACGGCGTATACCTCCGCTCTCGCACCGATGATGATTGCTGCTgcgc                                   >  2:1784159/1‑149 (MQ=255)
                                                                                                                  ggCAAACTGTTAGCAGGCAGCTTAAGCGGTTATTCGCCAACGATGGTGATGGTGGCGTTGATTGTGGTGTTTTATCTACTGCGCTACTTTTTCGCCAGCGCCACGGCGTATACCTCCGCTCTCGCACCTATGATGATTGCTGCTGCGCt                                  <  2:936698/149‑1 (MQ=255)
                                                                                                                  ggCAAACTGTTAGCAGGCAGCTTAAGCGGTTATTCGCCAACGATGGTGATGGTGGCGTTGATTGTGGTGTTTTATCTACTGCGCTACTTTTTCGCCAGCGCCACGGCGTATACCTCCGCTCTCGCACCGATGATGATTGCTGCTGCGCt                                  >  2:1686970/1‑149 (MQ=255)
                                                                                                                  ggCAAACTGTTAGCAGGCAGCTTAAGCGGTTATTCGCCAACGATGGTGATGGTGGCGTTGATTGTGGTGTTTTATCTACTGCGCTACTTTTTCGCCAGCGCCACGGCGTATACCTCCGCTCTCGCACCGATGATGATTGCTGCTGCGCt                                  >  2:4411263/1‑149 (MQ=255)
                                                                                                                   gCAAACTGTTAGCAGGCAGCTTAAGCGGTTATTCGCCAACGATGGTGATGGTGGCGTTGATTGTGGTGTTTTATCTACTGCGCTACTTTTTCGCCAGCGCCACGGCGTATACCTCCGCTCTCGCACCGATGATGATTGCTGCTGCGCTg                                 >  1:1080292/1‑149 (MQ=255)
                                                                                                                   gCAAACTGTTAGCAGGCAGCTTAAGCGGTTATTAGCCAACGATGGTGATGGTGGCGTTGATTGTGGTGTTTTATCTACTGCGCTACTTTTTCGCCAGCGCCACGGCGTATACCTCCGCTCTCGCACCGATGATGATTGCTGCTGCGCTg                                 >  1:4884457/1‑149 (MQ=255)
                                                                                                                    cAAACTGTTAGCAGGCAGCTTAAGCGGTTATTCGCCAACGATGGTGATGGTGGCGTTGATTGTGGTGTTTTATCTACTGCGCTACTTTTTCGCCAGCGCCACGGCGTATACCTCCGCTCTCGCACCGATGATGACTGCTGCTGCGCTgg                                >  1:2689987/1‑149 (MQ=255)
                                                                                                                     aaaCTGTTAGCAGGCAGCTTAAGCGGTTATTCGCCAACGATGGTGATGGTGGCGTTGATTGTGGTGTTTTATCTACTGCGCTACTTTTTCGCCAGCGCCACGGCGTATACCTCCGCTCTCGCACCGATGATGATTGCTGCTGCGCTGGc                               <  2:744110/149‑1 (MQ=255)
                                                                                                                          gTTAGCAGGCAGCTTAAGCGGTTATTCGCCAACGATGGTGATGGTGGCGTTGATTGTGGTGTTTTATCTACTGCGCTACTTTTTCGCCAGCGCCACGGCGTATACCTCCGCTCTCGCACCGATGATGATTGCTGCTGCGCTGGCGATGc                          <  1:1179378/149‑1 (MQ=255)
                                                                                                                             aGCAGGCAGCTTAAGCGGTTATTCGCCAACGATGGTGATGGTGGCGTTGATTGTGGTGTTTTATCTACTGCGCTACTTTTTCGCCAGCGCCACGGCGTATACCTCCGCTCTCGCACCGATGATGATTGCTGCTGCGCTGGCGATGCCgg                       >  1:3930372/1‑149 (MQ=255)
                                                                                                                             aGCAGGCAGCTTAAGCGGTTATTCGCCAACGATGGTGATGGTGGCGTTGATTGTGGTGTTTTATCTACTGCGCTACTTTTTCGCCAGCGCCACGGCGTATACCTCCGCTCTCGCACCGATGATGATTGCTGCTGCGCTGGCGATGCCgg                       >  2:567528/1‑149 (MQ=255)
                                                                                                                              gcaggcagCTTAAGCGGTTATTCGCCAACGATGGTGATGGTGGCGTTGATTGTGGTGTTTTATCTACTGCGCTACTTTTTCGCCAGCGCCACGGCGTATACCTCCGCTCTCGCACCGATGATGATTGCTGCTGCGCTGGCGATGCCGGa                      >  2:1430013/1‑149 (MQ=255)
                                                                                                                               caggcagCTTAAGCGGTTATTCGCCAACGATGGTGATGGTGGCGTTGATTGTGGTGTTTTATCTACTGCGCTACTTTTTCGCCAGCGCCACGGCGTATACCTCCGCTCTCGCACCGATGATGATTGCTGCTGCGCTGGCGATGCCGGaa                     <  2:1471225/149‑1 (MQ=255)
                                                                                                                                aggcagCTTAAGCGGTTATTCGCCAACGATGGTGATGGTGGCGTTGATTGTGGTGTTTTATCTACTGCGCTACTTTTTCGCCAGCGCCACGGCGTATACCTCCGCTCTCGCACCGATGATGATTGCTGCTGCGCTGGCGATGCCGGaaa                    <  2:3556583/149‑1 (MQ=255)
                                                                                                                                aggcagCTTAAGCGGTTATTCGCCAACGATGGTGATGGTGGCGTTGATTGTGGTGTTTTATCTACTGCGCTACTTTTTCGCCAGCGCCACGGCGTATACCTCCGCTCTCGCACCGATGATGATTGCTGCTGCGCTGGCGATGCCGGaaa                    >  2:1485125/1‑149 (MQ=255)
                                                                                                                                  gcagCTTAAGCGGTTATTCGCCAACGATGGTGATGGTGGCGTTGATTGTGGTGTTTTATCTACTGCGCTACTTTTTCGCCAGCGCCACGGCGTATACCTCCGCTCTCGCACCGATGATGATTGCTGCTGCGCTGGCGATGCCGGAAATc                  <  1:1297696/149‑1 (MQ=255)
                                                                                                                                          aGCGGTTATTAGCCAACGATGGTGATGGTGGCGTTGATTGTGGTGTTTTATCTACTGCGCTACTTTTTCGCCAGCGCCACGGCGTATACCTCCGCTCTCGCACCGATGATGATTGCTGCTGCGCTGGCGATGCCGGAAATCCCGCTGcc          <  2:4658718/149‑1 (MQ=255)
                                                                                                                                           gCGGTTATTCGCCAACGATGGTGATGGTGGCGTTGATTGTGGTGTTTTATCTACTGCGCTACTTTTTCGCCAGCGCCACGGCGTATACCTCCGCTCTCGCACCGATGATGATTGCTGCTGCGCTGGCGATGCCGGAAATCCCGCTGCCg         >  1:2346148/1‑149 (MQ=255)
                                                                                                                                             ggTTATTCGCCAACGATGGTGATGGTGGCGTTGATTGTGGTGTTTTATCTACTGCGCTACTTTTTCGCCAGCGCCACGGCGTATACCTCCGCTCTCGCACCGATGATGATTGCTGCTGCGCTGGCGATGCCGGAAATCCCGCTGCCGgt       <  1:4503864/149‑1 (MQ=255)
                                                                                                                                              gTTATTCGCCAACGATGGTGATGGTGGCGTTGATTGTGGTGTTTTATCTACTGCGCTACTTTTTCGCCAGCGCCACGGCGTATACCTCCGCTCTCGCACCGATGATGATTGCTGCTGCGCTGGCGATGCCGGAAATCCCGCTGCCGgtg      >  2:4867055/1‑149 (MQ=255)
                                                                                                                                                tATTCGCCAACGATGGTGATGGTGGCGTTGATTGTGGTGTTTTATCTACTGCGCTACTTTTTCGCCAGCGCCACGGCGTATACCTCCGCTCTCGCACCGATGATGATTGCTGCTGCGCTGGCGATGCCGGAAATCCCGCTGCCGGTGtt    >  1:3597834/1‑149 (MQ=255)
                                                                                                                                                 aTTCGCCAACGATGGTGATGGTGGCGTTGATTGTGGTGTTTTATCTACTGCGCTACTTTTTCGCCAGCGCCACGGCGTATACCTCCGCTCTCGCACCGATGATGATTGCTGCTGCGCTGGCGATGCCGGAAATCCCGCTGCCGGTGTTc   <  2:5116600/149‑1 (MQ=255)
                                                                                                                                                  ttCGCCAACGATGGTGATGGTGGCGTTGATTGTGGTGTTTTATCTACTGCGCTACTTTTTCGCCAGCGCCACGGCGTATACCTCCGCTCTCGCACCGATGATGATTGCTGCTGCGCTGGCGATGCCGGAAATCCCGCTGCCGGTGTTCt  >  1:2975657/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
ATCAGTTGGGACGACATTGTCAGTAATAAAGCGGCGTGGAACGTTTTCTTCTGGCTGGCCTCGCTTATCACCCTCGCCACCGGACTCAACAACACCGGTTTTATTAGCTGGTTTGGCAAACTGTTAGCAGGCAGCTTAAGCGGTTATTCGCCAACGATGGTGATGGTGGCGTTGATTGTGGTGTTTTATCTACTGCGCTACTTTTTCGCCAGCGCCACGGCGTATACCTCCGCTCTCGCACCGATGATGATTGCTGCTGCGCTGGCGATGCCGGAAATCCCGCTGCCGGTGTTCT  >  CP009273/3202358‑3202652

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑