Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,302,027 G→T 81.1% S86* (TCG→TAG)  nlpI ← lipoprotein involved in osmotic sensitivity and filamentation

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,302,0270GT81.1% 535.5 / 153.1 238S86* (TCG→TAG) nlpIlipoprotein involved in osmotic sensitivity and filamentation
Reads supporting (aligned to +/- strand):  ref base G (24/21);  new base T (109/84);  total (133/105)
Fisher's exact test for biased strand distribution p-value = 7.41e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.99e-01

Only 100 of 240 total aligned reads displayed.

ATTCAAGTGCGCGTAGTTGTAAGTTGGATCAAGCTCAAGTACAGAATCAAACGCTTCATAGGCAGCATCAAAATTGCCTGCCTGCGTTAAATATATGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCGAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCCGCGCTCATATAAAAGCTGTGCGCGTTCGTCATCGGTTAAAGCCCGACTGGCAAGGATTTGTTCCATACGTGCCAGAATCACTTCCTGCTGTAAAGT  >  CP009273/3301879‑3302175
                                                                                                                                                    |                                                                                                                                                    
   cAAGTGCGCGTAGTTGTAAGTTGGATCAAGCTCAAGTACAGAATCAAACGCTTCATAGGCAGCATCAAAATTGCCTGCCTGCGTTAAATATATGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTaaa                                                                                                                                                   <  2:4044773/149‑1 (MQ=255)
     aGTGCGCGTAGTTGTAAGTTGGATCAAGCTCAAGTACAGAATCAAACGCTTCATAGGCAGCATCAAAATTGCCTGCCTGCGTTAAATATATGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTaaaaa                                                                                                                                                 >  1:1073788/1‑149 (MQ=255)
     aGTGCGCGTAGTTGTAAGTTGGATCAAGCTCAAGTACAGAATCAAACGCTTCATAGGCAGCATCAAAATTGCCTGCCTGCGTTAAATATATGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTaaaaa                                                                                                                                                 <  2:5030332/149‑1 (MQ=255)
     aGTGCGCGTAGTTGTAAGTTGGATCAAGCTCAAGTACAGAATCAAACGCTTCATAGGCAGCATCAAAATTGCCTGCCTGCGTTAAATATATGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCGaaaaa                                                                                                                                                 <  1:259744/149‑1 (MQ=255)
         cgcgTAGTTGTAAGTTGGATCAAGCTCAAGTACAGAATCAAACGCTTCATAGGCAGCATCAAAATTGCCTGCCTGCGTTAAATATATGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCGAAAAATCGt                                                                                                                                             <  1:4567636/149‑1 (MQ=255)
           cgTAGTTGTAAGTTGGATCAAGCTCAAGTACAGAATCAAACGCTTCATAGGCAGCATCAAAATTGCCTGCCTGCGTTAAATATATGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTa                                                                                                                                           <  2:3690216/149‑1 (MQ=255)
            gTAGTTGTAAGTTGGATCAAGCTCAAGTACAGAATCAAACGCTTCATAGGCAGCATCAAAATTGCCTGCCTGCGTTAAATATATGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTAc                                                                                                                                          >  1:1969313/1‑149 (MQ=255)
            gTAGTTGTAAGTTGGATCAAGCTCAAGTACAGAATCAAACGCTTCATAGGCAGCATCAAAATTGCCTGCCTGCGTTAAATATATGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTAc                                                                                                                                          >  1:3370461/1‑149 (MQ=255)
                ttGTAAGTTGGATCAAGCTCAAGTACAGAATCAAACGCTTCATAGGCAGCATCAAAATTGCCTGCCTGCGTTAAATATATGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACacgc                                                                                                                                      >  2:2959681/1‑149 (MQ=255)
                  gTAAGTTGGATCAAGCTCAAGTACAGAATCAAACGCTTCATAGGCAGCATCAAAATTGCCTGCCTGCGTTAAATATATGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCGAAAAATCGTTACGCGCTa                                                                                                                                    <  2:2573200/149‑1 (MQ=255)
                    aaGTTGGATCAAGCTCAAGTACAGAATCAAACGCTTCATAGGCAGCATCAAAATTGCCTGCCTGCGTTAAATATATGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAAt                                                                                                                                  <  2:1863628/149‑1 (MQ=255)
                      gTTGGATCAAGCTCAAGTACAGAATCAAACGCTTCATAGGCAGCATCAAAATTGCCTGCCTGCGTTAAATATATGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCGAAAAATCGTTACGCGCTAATGc                                                                                                                                >  2:4700261/1‑149 (MQ=255)
                      gTTGGATCAAGCTCAAGTACAGAATCAAACGCTTCATAGGCAGCATCAAAATTGCCTGCCTGCGTTAAATATATGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCGAAAAATCGTTACGCGCTAATGc                                                                                                                                <  1:1541173/149‑1 (MQ=255)
                       ttGGATCAAGCTCAAGTACAGAATCAAACGCTTCATAGGCAGCATCAAAATTGCCTGCCTGCGTTAAATATATGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGcc                                                                                                                               >  2:1907192/1‑149 (MQ=255)
                        tGGATCAAGCTCAAGTACAGAATCAAACGCTTCATAGGCAGCATCAAAATTGCCTGCCTGCGTTAAATATATGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGccc                                                                                                                              >  2:460288/1‑149 (MQ=255)
                            tCAAGCTCAAGTACAGAATCAAACGCTTCATAGGCAGCATCAAAATTGCCTGCCTGCGTTAAATATATGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAg                                                                                                                          >  1:2396364/1‑149 (MQ=255)
                              aaGCTCAAGTACAGAATCAAACGCTTCATAGGCAGCATCAAAATTGCCTGCCTGCGTTAAATATATGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGAc                                                                                                                        <  2:417331/149‑1 (MQ=255)
                              aaGCTCAAGTACAGAATCAAACGCTTCATAGGCAGCATCAAAATTGCCTGCCTGCGTTAAATATATGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCGAAAAATCGTTACGCGCTAATGCCCTCAGAc                                                                                                                        <  2:1763667/149‑1 (MQ=255)
                               aGCTCAAGTACAGAATCAAACGCTTCATAGGCAGCATCAAAATTGCCTGCCTGCGTTAAATATATGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGAcc                                                                                                                       >  2:4999579/1‑149 (MQ=255)
                                gCTCAAGTACAGAATCAAACGCTTCATAGGCAGCATCAAAATTGCCTGCCTGCGTTAAATATATGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCg                                                                                                                      <  2:2087778/149‑1 (MQ=255)
                                gCTCAAGTACAGAATCAAACGCTTCATAGGCAGCATCAAAATTGCCTGCCTGCGTTAAATATATGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCg                                                                                                                      >  2:3808030/1‑149 (MQ=255)
                                  tCAAGTACAGAATCAAACGCTTCATAGGCAGCATCAAAATTGCCTGCCTGCGTTAAATATATGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCgag                                                                                                                    >  2:1659595/1‑149 (MQ=255)
                                  tCAAGTACAGAATCAAACGCTTCATAGGCAGCATCAAAATTGCCTGCCTGCGTTAAATATATGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCgag                                                                                                                    >  2:1659195/1‑149 (MQ=255)
                                    aaGTACAGAATCAAACGCTTCATAGGCAGCATCAAAATTGCCTGCCTGCGTTAAATATATGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGAc                                                                                                                  <  2:1311711/149‑1 (MQ=255)
                                    aaGTACAGAATCAAACGCTTCATAGGCAGCATCAAAATTGCCTGCCTGCGTTAAATATATGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGAc                                                                                                                  <  2:1311601/149‑1 (MQ=255)
                                       tACAGAATCAAACGCTTCATAGGCAGCATCAAAATTGCCTGCCTGCGTTAAATATATGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTAt                                                                                                               <  2:443698/149‑1 (MQ=255)
                                        aCAGAATCAAACGCTTCATAGGCAGCATCAAAATTGCCTGCCTGCGTTAAATATATGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATc                                                                                                              <  1:3140843/149‑1 (MQ=255)
                                           gAATCAAACGCTTCATAGGCAGCATCAAAATTGCCTGCCTGCGTTAAATATATGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATa                                                                                                           >  1:5020526/1‑149 (MQ=255)
                                           gAATCAAACGCTTCATAGGCAGCATCAAAATTGCCTGCCTGCGTTAAATATATGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCGAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATa                                                                                                           >  2:4317584/1‑149 (MQ=255)
                                            aaTCAAACGCTTCATAGGCAGCATCAAAATTGCCTGCCTGCGTTAAATATATGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATac                                                                                                          >  2:3417573/1‑149 (MQ=255)
                                              tCAAACGCTTCATAGGCAGCATCAAAATTGCCTGCCTGCGTTAAATATATGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCGAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATacaa                                                                                                        <  1:4779760/149‑1 (MQ=255)
                                               cAAACGCTTCATAGGCAGCATCAAAATTGCCTGCCTGCGTTAAATATATGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATacaac                                                                                                       >  2:29542/1‑149 (MQ=255)
                                               cAAACGCTTCATAGGCAGCATCAAAATTGCCTGCCTGCGTTAAATATATGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCGAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATacaac                                                                                                       >  1:411984/1‑149 (MQ=255)
                                                 aaCGCTTCATAGGCAGCATCAAAATTGCCTGCCTGCGTTAAATATATGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAacac                                                                                                     >  1:791177/1‑149 (MQ=255)
                                                   cGCTTCATAGGCAGCATCAAAATTGCCTGCCTGCGTTAAATATATGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTc                                                                                                   <  1:5225283/149‑1 (MQ=255)
                                                    gCTTCATAGGCAGCATCAAAATTGCCTGCCTGCGTTAAATATATGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTcc                                                                                                  >  1:3263189/1‑149 (MQ=255)
                                                      ttCATAGGCAGCATCAAAATTGCCTGCCTGCGTTAAATATATGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCcgc                                                                                                <  2:2162930/149‑1 (MQ=255)
                                                        cATAGGCAGCATCAAAATTGCCTGCCTGCGTTAAATATATGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCCgcgc                                                                                              <  1:696593/149‑1 (MQ=255)
                                                         aTAGGCAGCATCAAAATTGCCTGCCTGCGTTAAATATATGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCCGCGCt                                                                                             >  2:3360714/1‑149 (MQ=255)
                                                         aTAGGCAGCATCAAAATTGCCTGCCTGCGTTAAATATATGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCGAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCCGCGCt                                                                                             >  1:369382/1‑149 (MQ=255)
                                                           aGGCAGCATCAAAATTGCCTGCCTGCGTTAAATATATGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCCGCGCTCa                                                                                           >  1:5184565/1‑149 (MQ=255)
                                                             gcagcaTCAAAATTGCCTGCCTGCGTTAAATATATGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCCGCGCTCata                                                                                         <  2:3929711/149‑1 (MQ=255)
                                                               agcaTCAAAATTGCCTGCCTGCGTTAAATATATGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCCGCGCTCAtata                                                                                       <  2:36846/149‑1 (MQ=255)
                                                                gcaTCAAAATTGCCTGCCTGCGTTAAATATATGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCCGCGCTCATATaa                                                                                      >  1:2299516/1‑149 (MQ=255)
                                                                  aTCAAAATTGCCTGCCTGCGTTAAATATATGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCCGCGCTCATATaaaa                                                                                    >  1:21129/1‑149 (MQ=255)
                                                                  aTCAAAATTGCCTGCCTGCGTTAAATATATGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCCGCGCTCATATaaaa                                                                                    >  2:577338/1‑149 (MQ=255)
                                                                  aTCAAAATTGCCTGCCTGCGTTAAATATATGCCTAAGTAATTGAATACTTCAGGCAAATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTAAGCGCTAAAGCCATCAGACCGAGACTATCAAACAACACTCCGCTCTCATATaaaa                                                                                    >  1:20921/1‑149 (MQ=255)
                                                                     aaaaTTGCCTGCCTGCGTTAAATATATGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCCGCGCTCATATAAAAGCt                                                                                 <  2:4731599/149‑1 (MQ=255)
                                                                      aaaTTGCCTGCCTGCGTTAAATATATGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCCGCGCTCATATAAAAGCtg                                                                                >  1:3560346/1‑149 (MQ=255)
                                                                        aTTGCCTGCCTGCGTTAAATATATGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCCGCGCTCATATAAAAGCtgtg                                                                              <  2:2400181/149‑1 (MQ=255)
                                                                               gcctgcGTTAAATATATGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCGAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCCGCGCTCATATAAAAGCTGTGCGCGTTc                                                                       <  1:2486507/149‑1 (MQ=255)
                                                                               gcctgcGTTAAATATATGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCGAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCCGCGCTCATATAAAAGCTGTGCGCGTTc                                                                       <  1:3177680/149‑1 (MQ=255)
                                                                                  tgcGTTAAATATATGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCCGCGCTCATATAAAAGCTGTGCGCGTTCGTc                                                                    >  1:32796/1‑149 (MQ=255)
                                                                                     gTTAAATATATGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACAATCCGCGCTCATATAAAAGCTGTGCGCGTTCGTCATc                                                                 >  1:2686620/1‑149 (MQ=255)
                                                                                      ttAAATATATGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCCGCGCTCATATAAAAGCTGTGCGCGTTCGTCATCg                                                                >  2:446558/1‑149 (MQ=255)
                                                                                      ttAAATATATGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCCGCGCTCATATAAAAGCTGTGCGCGTTCGTCATCg                                                                >  1:99573/1‑149 (MQ=255)
                                                                                       tAAATATATGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCCGCGCTCATATAAAAGCTGTGCGCGTTCGTCATCgg                                                               <  1:3928863/149‑1 (MQ=255)
                                                                                         aaTATATGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCGAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCCGCGCTCATATAAAAGCTGTGCGCGTTCGTCATCGGtt                                                             >  2:1505533/1‑149 (MQ=255)
                                                                                          atatatGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCCGCGCTCATATAAAAGCTGTGCGCGTTCGTCATCGGTTa                                                            >  2:1455507/1‑149 (MQ=255)
                                                                                          atatatGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCCGCGCTCATATAAAAGCTGTGCGCGTTCGTCATCGGTTa                                                            >  2:1075561/1‑149 (MQ=255)
                                                                                          atatatGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCCGCGCTCATATAAAAGCTGTGCGCGTTCGTCATCGGTTa                                                            >  2:3667047/1‑149 (MQ=255)
                                                                                           tatatGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTCATGCCCTCAGACCGAGACTATCATACAACACTCCGCGCTCATATAAAAGCTGTGCGCGTTCGTCATCGGTTaa                                                           >  2:1070689/1‑149 (MQ=255)
                                                                                             tatGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCCGCGCTCATATAAAAGCTGTGCGCGTTCGTCATCGGTTAAAg                                                         >  2:506485/1‑149 (MQ=255)
                                                                                             tatGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCCGCGCTCATATAAAAGCTGTGCGCGTTCGTCATCGGTTAAAg                                                         >  1:119747/1‑149 (MQ=255)
                                                                                              atGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCCGCGCTCATATAAAAGCTGTGCGCGTTCGTCATCGGTTAAAGc                                                        >  2:3147639/1‑149 (MQ=255)
                                                                                                gCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCGAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCCGCGCTCATATAAAAGCTGTGCGCGTTCGTCATCGGTTAAAGccc                                                      >  2:4981364/1‑149 (MQ=255)
                                                                                                 ccTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCCGCGCTCATATAAAAGCTGTGCGCGTTCGTCATCGGTTAAAGCCCg                                                     <  1:4088292/149‑1 (MQ=255)
                                                                                                 ccTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCCGCGCTCATATAAAAGCTGTGCGCGTTCGTCATCGGTTAAAGCCCg                                                     >  1:3841581/1‑149 (MQ=255)
                                                                                                   tAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCCGCGCTCATATAAAAGCTGTGCGCGTTCGTCATCGGTTAAAGCCCGAc                                                   <  2:2709563/149‑1 (MQ=255)
                                                                                                    aaGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCCGCGCTCATATAAAAGCTGTGCGCGTTCGTCATCGGTTAAAGCCCGACt                                                  >  2:3764883/1‑149 (MQ=255)
                                                                                                        aaTTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCCGCGCTCATATAAAAGCTGTGCGCGTTCGTCATCGGTTAAAGCCCGACTGGCa                                              >  2:1689299/1‑149 (MQ=255)
                                                                                                        aaTTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCCGCGCTCATATAAAAGCTGTGCGCGTTCGTCATCGGTTAAAGCCCGACTGGCa                                              <  1:3139181/149‑1 (MQ=255)
                                                                                                           tGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCCGCGCTCATATAAAAGCTGTGCGCGTTCGTCATCGGTTAAAGCCCGACTGGCAAgg                                           <  1:2186341/149‑1 (MQ=255)
                                                                                                              aTACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCCGCGCTCATATAAAAGCTGTGCGCGTTCGTCATCGGTTAAAGCCCGACTGGCAAGGAtt                                        >  1:2284386/1‑149 (MQ=255)
                                                                                                              aTACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCGAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCCGCGCTCATATAAAAGCTGTGCGCGTTCGTCATCGGTTAAAGCCCGACTGGCAAGGAtt                                        <  2:1195797/149‑1 (MQ=255)
                                                                                                               tACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCCGCGCTCATATAAAAGCTGTGCGCGTTCGTCATCGGTTAAAGCCCGACTGGCAAGGAttt                                       >  2:4842466/1‑149 (MQ=255)
                                                                                                                 cTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCCGCGCTCATATAAAAGCTGTGCGCGTTCGTCATCGGTTAAAGCCCGACTGGCAAGGATTTGt                                     >  1:1642403/1‑149 (MQ=255)
                                                                                                                 cTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCGAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCCGCGCTCATATAAAAGCTGTGCGCGTTCGTCATCGGTTAAAGCCCGACTGGCAAGGATTTGt                                     <  2:5110173/149‑1 (MQ=255)
                                                                                                                   tCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCCGCGCTCATATAAAAGCTGTGCGCGTTCGTCATCGGTTAAAGCCCGACTGGCAAGGATTTGTTc                                   <  1:1148371/149‑1 (MQ=255)
                                                                                                                   tCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCCGCGCTCATATAAAAGCTGTGCGCGTTCGTCATCGGTTAAAGCCCGACTGGCAAGGATTTGTTc                                   <  1:3411655/149‑1 (MQ=255)
                                                                                                                    cAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCCGCGCTCATATAAAAGCTGTGCGCGTTCGTCATCGGTTAAAGCCCGACTGGCAAGGATTTGTTcc                                  <  2:3963727/149‑1 (MQ=255)
                                                                                                                     aGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCCGCGCTCATATAAAAGCTGTGCGCGTTCGTCATCGGTTAAAGCCCGACTGGCAAGGATTTGTTCCa                                 >  1:1737246/1‑149 (MQ=255)
                                                                                                                     aGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCCGCGCTCATATAAAAGCTGTGCGCGTTCGTCATCGGTTAAAGCCCGACTGGCAAGGATTTGTTCCa                                 <  1:2131421/149‑1 (MQ=255)
                                                                                                                     aGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAAGGCCCTCAGACCGAGACTATCATACAACACTCCGCGCTCATATAAAAGCTGTGCGCGTTCGTCATCGGTTAAAGCCCGACTGGCAAGGATTTGTTCCa                                 <  1:2770801/149‑1 (MQ=255)
                                                                                                                       gCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCCGCGCTCATATAAAAGCTGTGCGCGTTCGTCATCGGTTAAAGCCCGACTGGCAAGGATTTGTTCCATa                               >  1:860169/1‑149 (MQ=255)
                                                                                                                        cATATCCGGTCGGATTGCCAGCGCTTGCGAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCCGCGCTCATATAAAAGCTGTGCGCGTTCGTCATCGGTTAAAGCCCGACTGGCAAGGATTTGTTCCATAc                              >  1:5151233/1‑149 (MQ=255)
                                                                                                                        cATATCCGGTCGGATTGCCAGCGCTTGCGAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCCGCGCTCATATAAAAGCTGTGCGCGTTCGTCATCGGTTAAAGCCCGACTGGCAAGGATTTGTTCCATAc                              <  1:4700261/149‑1 (MQ=255)
                                                                                                                         atatCCGGTCGGATTGCCAGCGCTTGCGAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCCGCGCTCATATAAAAGCTGTGCGCGTTCGTCATCGGTTAAAGCCCGACTGGCAAGGATTTGTTCCATACg                             <  2:1402226/149‑1 (MQ=255)
                                                                                                                           atCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCCGCGCTCATATAAAAGCTGTGCGCGTTCGTCATCGGTTAAAGCCCGACTGGCAAGGATTTGTTCCATACGTg                           >  2:24354/1‑149 (MQ=255)
                                                                                                                             ccGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCCGCGCTCATATAAAAGCTGTGCGCGTTCGTCATCGGTTAAAGCCCGACTGGCAAGGATTTGTTCCATACGTGcc                         >  2:1229844/1‑149 (MQ=255)
                                                                                                                             ccGGTCGGATTGCCAGCGCTTGCGAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCCGCGCTCATATAAAAGCTGTGCGCGTTCGTCATCGGTTAAAGCCCGACTGGCAAGGATTTGTTCCATACGTGcc                         <  2:211683/149‑1 (MQ=255)
                                                                                                                              cGGTCGGATTGCCAGCGCTTGCGAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCCGCGCTCATATAAAAGCTGTGCGCGTTCGTCATCGGTTAAAGCCCGACTGGCAAGGATTTGTTCCATACGTGCCa                        >  2:880345/1‑149 (MQ=255)
                                                                                                                                gTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCCGCGCTCATATAAAAGCTGTGCGCGTTCGTCATCGGTTAAAGCCCGACTGGCAAGGATTTGTTCCATACGTGCCAGa                      >  2:2650424/1‑149 (MQ=255)
                                                                                                                                  cGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCCGCGCTCATATAAAAGCTGTGCGCGTTCGTCATCGGTTAAAGCCCGACTGGCAAGGATTTGTTCCATACGTGCCAGAAt                    <  2:1112248/149‑1 (MQ=255)
                                                                                                                                  cGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCCGCGCTCATATAAAAGCTGTGCGCGTTCGTCATCGGTTAAAGCCCGACTGGCAAGGATTTGTTCCATACGTGCCAGAAt                    <  2:4371564/149‑1 (MQ=255)
                                                                                                                                   ggATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCCGCGCTCATATAAAAGCTGTGCGCGTTCGTCATCGGTTAAAGCCCGACTGGCAAGGAGTTGTTCCATACGTGCCAGAATc                   >  2:72098/1‑149 (MQ=255)
                                                                                                                                        gCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCCGCGCTCATATAAAAGCTGTGCGCGTTCGTCATCGGTTAAAGCCCGACTGGCAAGGATTTGTTCCATACGTGCCAGAATCACTTc              <  1:1341936/149‑1 (MQ=255)
                                                                                                                                           aGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCCGCGCTCATATAAAAGCTGTGCGCGTTCGTCATCGGTTAAAGCCCGACTGGCAAGGATTTGTTCCATACGTGCCAGAATCACTTCctg           >  1:608648/1‑149 (MQ=255)
                                                                                                                                            gcgcTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCCGCGCTCATATAAAAGCTGTGCGCGTTCGTCATCGGTTAAAGCCCGACTGGCAAGGATTTGTTCCATACGTGCCAGAATCACTTCctgc          <  1:1990200/149‑1 (MQ=255)
                                                                                                                                            gcgcTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCCGCGCTCATATAAAAGCTGTGCGCGTTCGTCATCGGTTAAAGCCCGACTGGCAAGGATTTGTTCCATACGTGCCAGAATCACTTCctgc          <  2:1610729/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
ATTCAAGTGCGCGTAGTTGTAAGTTGGATCAAGCTCAAGTACAGAATCAAACGCTTCATAGGCAGCATCAAAATTGCCTGCCTGCGTTAAATATATGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCGAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCCGCGCTCATATAAAAGCTGTGCGCGTTCGTCATCGGTTAAAGCCCGACTGGCAAGGATTTGTTCCATACGTGCCAGAATCACTTCCTGCTGTAAAGT  >  CP009273/3301879‑3302175

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑