Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,561,574 C→A 84.5% W372L (TGG→TTG)  glgC ← glucose‑1‑phosphate adenylyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,561,5740CA84.5% 861.8 / 166.4 340W372L (TGG→TTG) glgCglucose‑1‑phosphate adenylyltransferase
Reads supporting (aligned to +/- strand):  ref base C (30/23);  new base A (147/140);  total (177/163)
Fisher's exact test for biased strand distribution p-value = 5.50e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.80e-01

Only 100 of 342 total aligned reads displayed.

ATTTCGCGCGTTACCAGCACGATGCCTTCTTCTGAACGATAGAAACGACGTGCATCTTCCTCTGCGTTTTCACCAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCCATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGGAGATCACACAACCGCCGGAAACCAGTGAGTTAAGGGTCATCCCGTGGCTACCG  >  CP009273/3561427‑3561720
                                                                                                                                                   |                                                                                                                                                  
  ttCGCGCGTTACCAGCACGATGCCTTCTTCTGAACGATAGAAACGACGTGCATCTTCCTCTGCGTTTTCACCAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATa                                                                                                                                                 <  1:2309461/149‑1 (MQ=255)
   tCGCGCGTTACCAGCACGATGCCTTCTTCTGAACGATAGAAACGACGTGCATCTTCCTCTGCGTTTTCACCAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCCATAc                                                                                                                                                <  2:2598753/149‑1 (MQ=255)
    cgcgcgTTACCAGCACGATGCCTTCTTCTGAACGATAGAAACGACGTGCATCTTCCTCTGCGTTTTCACCAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACt                                                                                                                                               <  2:5084758/149‑1 (MQ=255)
    cgcgcgTTACCAGCACGATGCCTTCTTCTGAACGATAGAAACGACGTGCATCTTCCTCTGCGTTTTCACCAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACt                                                                                                                                               <  2:1118138/149‑1 (MQ=255)
     gcgcgTTACCAGCACGATGCCTTCTTCTGAACGATAGAAACGACGTGCATCTTCCTCTGCGTTTTCACCAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACtt                                                                                                                                              <  2:2522631/149‑1 (MQ=255)
       gcgTTACCAGCACGATGCCTTCTTCTGAACGATAGAAACGACGTGCATCTTCCTCTGCGTTTTCACCAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCCATACTTcc                                                                                                                                            >  1:1482467/1‑149 (MQ=255)
       gcgTTACCAGCACGATGCCTTCTTCTGAACGATAGAAACGACGTGCATCTTCCTCTGCGTTTTCACCAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTcc                                                                                                                                            >  1:3282162/1‑149 (MQ=255)
          ttACCAGCAAGATGCCTTCTTCTGACCTATAGAAACGACGTGCATCTTCCTCTGCGTTTTCACCAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCCATACTTCCGGt                                                                                                                                         <  1:893771/149‑1 (MQ=255)
           tACCAGCACGATGCCTTCTTCTGAACGATAGAAACGACGTGCATCTTCCTCTGCGTTTTCACCAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTa                                                                                                                                        <  2:250253/149‑1 (MQ=255)
           tACCAGCACGATGCCTTCTTCTGAACGATAGAAACGACGTGCATCTTCCTCTGCGTTTTCACCAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTa                                                                                                                                        >  1:1698483/1‑149 (MQ=255)
            aCCAGCACGATGCCTTCTTCTGAACGATAGAAACGACGTGCATCTTCCTCTGCGTTTTCACCAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTaa                                                                                                                                       <  2:1247510/149‑1 (MQ=255)
            aCCAGCACGATGCCTTCTTCTGAACGATAGAAACGACGTGCATCTTCCTCTGCGTTTTCACCAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTaa                                                                                                                                       <  2:1192885/149‑1 (MQ=255)
             ccAGCACGATGCCTTCTTCTGAACGATAGAAACGACGTGCATCTTCCTCTGCGTTTTCACCAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAAc                                                                                                                                      >  2:2168894/1‑149 (MQ=255)
              cAGCACGATGCCTTCTTCTGAACGATAGAAACGACGTGCATCTTCCTCTGCGTTTTCACCAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACa                                                                                                                                     >  1:902450/1‑149 (MQ=255)
               aGCACGATGCCTTCTTCTGAACGATAGAAACGACGTGCATCTTCCTCTGCGTTTTCACCAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCCATACTTCCGGTAACaa                                                                                                                                    >  1:2821550/1‑149 (MQ=255)
               aGCACGATGCCTTCTTCTGAACGATAGAAACGACGTGCATCTTCCTCTGCGTTTTCACCAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACaa                                                                                                                                    >  2:934224/1‑149 (MQ=255)
                 cACGATGCCTTCTTCTGAACGATAGAAACGACGTGCATCTTCCTCTGCGTTTTCACCAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATa                                                                                                                                  >  1:4680392/1‑149 (MQ=255)
                  aCGATGCCTTCTTCTGAACGATAGAAACGACGTGCATCTTCCTCTGCGTTTTCACCAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATAc                                                                                                                                 >  1:3168121/1‑149 (MQ=255)
                    gATGCCTTCTTCTGAACGATAGAAACGACGTGCATCTTCCTCTGCGTTTTCACCAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATAcgg                                                                                                                               >  2:2015256/1‑149 (MQ=255)
                     aTGCCTTCTTCTGAACGATAGAAACGACGTGCATCTTCCTCTGCGTTTTCACCAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATAcggc                                                                                                                              >  2:2428741/1‑149 (MQ=255)
                       gCCTTCTTCTGAACGATAGAAACGACGTGCATCTTCCTCTGCGTTTTCACCAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATAcggcgg                                                                                                                            <  1:2635562/149‑1 (MQ=255)
                       gCCTTCTTCTGAACGATAGAAACGACGTGCATCTTCCTCTGCGTTTTCACCAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATAcggcgg                                                                                                                            <  1:2161965/149‑1 (MQ=255)
                         cttcttCTGAACGATAGAAACGACGTGCATCTTCCTCTGCGTTTTCACCAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGaa                                                                                                                          <  2:2770344/149‑1 (MQ=255)
                         cttcttCTGAACGATAGAAACGACGTGCATCTTCCTCTGCGTTTTCACCAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGaa                                                                                                                          >  1:306739/1‑149 (MQ=255)
                          ttcttcTGAACGATAGAAACGACGTGCATCTTCCTCTGCGTTTTCACCAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCCATACTTCCGGTAACAATACGGCGGAAt                                                                                                                         <  1:3778658/149‑1 (MQ=255)
                           tcttctGAACGATAGACACGACGTGCATCTTCCTCTGCGTTTTCACCAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATc                                                                                                                        <  1:4021274/149‑1 (MQ=255)
                                tGAACGATAGAAACGACGTGCATCTTCCTCTGCGTTTTCACCAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGt                                                                                                                   >  2:4140031/1‑149 (MQ=255)
                                tGAACGATAGAAACGACGTGCATCTTCCTCTGCGTTTTCACCAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGt                                                                                                                   >  1:1004984/1‑149 (MQ=255)
                                  aaCGATAGAAACGACGTGCATCTTCCTCTGCGTTTTCACCAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTg                                                                                                                 >  1:4709981/1‑149 (MQ=255)
                                  aaCGATAGAAACGACGTGCATCTTCCTCTGCGTTTTCACCAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTg                                                                                                                 >  2:1580295/1‑149 (MQ=255)
                                    cGATAGAAACGACGTGCATCTTCCTCTGCGTTTTCACCAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCCATACTTCCGGTAACAATACGGCGGAATCAATGTTGCa                                                                                                               <  1:4355335/149‑1 (MQ=255)
                                       tAGAAACGACGTGCATCTTCCTCTGCGTTTTCACCAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAgaa                                                                                                            <  2:3810425/149‑1 (MQ=255)
                                        aGAAACGACGTGCATCTTCCTCTGCGTTTTCACCAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAgaat                                                                                                           <  2:1282153/149‑1 (MQ=255)
                                          aaaCGACGTGCATCTTCCTCTGCGTTTTCACCAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAgaatga                                                                                                         >  2:2472071/1‑149 (MQ=255)
                                           aaCGACGTGCATCTTCCTCTGCGTTTTCACCAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAgaatgaa                                                                                                        >  2:2228528/1‑149 (MQ=255)
                                           aaCGACGTGCATCTTCCTCTGCGTTTTCACCAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAgaatgaa                                                                                                        >  1:4114925/1‑149 (MQ=255)
                                           aaCGACGTGCATCTTCCTCTGCGTTTTCACCAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAgaatgaa                                                                                                        >  1:1497595/1‑149 (MQ=255)
                                           aaCGACGTGCATCTTCCTCTGCGTTTTCACCAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAgaatgaa                                                                                                        <  1:3869567/149‑1 (MQ=255)
                                               acgTGCATCTTCCTCTGCGTTTTCACCAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCa                                                                                                    >  2:2986629/1‑149 (MQ=255)
                                                  tGCATCTTCCTCTGCGTTTTCACCAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCAcgc                                                                                                 >  2:190763/1‑149 (MQ=255)
                                                       cTTCCTCTGCGTTTTCACCAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAAcg                                                                                            >  1:2525260/1‑149 (MQ=255)
                                                        ttCCTCTGCGTTTTCACCAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGACcgc                                                                                           >  2:169562/1‑149 (MQ=255)
                                                             ctGCGTTTTCACCAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCgag                                                                                      >  2:1372688/1‑149 (MQ=255)
                                                               gCGTTTTCACCAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGagaa                                                                                    >  2:3241973/1‑149 (MQ=255)
                                                               gCGTTTTCACCAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGagaa                                                                                    >  2:3107092/1‑149 (MQ=255)
                                                                  ttttCACCAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGagaacag                                                                                 <  1:4186419/149‑1 (MQ=255)
                                                                    ttCACCAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGagaacagaa                                                                               <  2:4026400/149‑1 (MQ=255)
                                                                     tCACCAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCCATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGagaacagaac                                                                              >  2:1531286/1‑149 (MQ=255)
                                                                       aCCAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCCATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACgg                                                                            >  1:3093664/1‑149 (MQ=255)
                                                                         cAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCCATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGAc                                                                          >  1:2929979/1‑149 (MQ=255)
                                                                         cAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGAGAACGGAACGGAc                                                                          >  1:2743662/1‑149 (MQ=255)
                                                                         cAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGAc                                                                          >  1:3908214/1‑149 (MQ=255)
                                                                           aTCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGACTg                                                                        >  2:3652914/1‑149 (MQ=255)
                                                                           aTCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGACTg                                                                        >  2:5239816/1‑149 (MQ=255)
                                                                            tCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGACTGc                                                                       <  1:2218542/149‑1 (MQ=255)
                                                                            tCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGACTGc                                                                       >  2:4573181/1‑149 (MQ=255)
                                                                                 aTGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGACTGcaccac                                                                  >  2:4374111/1‑149 (MQ=255)
                                                                                 aTGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGACTGcaccac                                                                  <  2:4997519/149‑1 (MQ=255)
                                                                                 aTGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGACTGcaccac                                                                  >  2:84489/1‑149 (MQ=255)
                                                                                  tGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGACTGcaccacc                                                                 >  2:1027121/1‑149 (MQ=255)
                                                                                  tGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGACTGcaccacc                                                                 >  2:1684530/1‑149 (MQ=255)
                                                                                   gCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGACTGcaccacca                                                                >  2:2930397/1‑149 (MQ=255)
                                                                                      ttCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCg                                                             <  2:3880308/149‑1 (MQ=255)
                                                                                         cGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGAAc                                                          <  2:715/149‑1 (MQ=255)
                                                                                          ggAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGTGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGAAcc                                                         <  1:2130779/149‑1 (MQ=255)
                                                                                           gAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCg                                                        >  1:3556423/1‑149 (MQ=255)
                                                                                             aTAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGACCCGga                                                      >  1:5179409/1‑149 (MQ=255)
                                                                                             aTAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGga                                                      <  2:306739/149‑1 (MQ=255)
                                                                                             aTAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGga                                                      <  2:3330653/149‑1 (MQ=255)
                                                                                             aTAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGga                                                      >  1:3694593/1‑149 (MQ=255)
                                                                                              tAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCCATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGgag                                                     >  1:1869665/1‑149 (MQ=255)
                                                                                                acacAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGGAGAt                                                   <  2:3826667/149‑1 (MQ=255)
                                                                                                   caAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGGAGATcac                                                >  1:3627603/1‑149 (MQ=255)
                                                                                                    aaGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGGAGATcaca                                               <  1:4853829/149‑1 (MQ=255)
                                                                                                     aGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCCATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGGAGATcacac                                              >  1:4915521/1‑149 (MQ=255)
                                                                                                      gCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCCCTACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGGAGATcacaca                                             <  1:1816752/149‑1 (MQ=255)
                                                                                                         cgatcgatGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGGAGATCACACAAcc                                          <  2:1041866/149‑1 (MQ=255)
                                                                                                         cgatcgatGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGGAGATCACACAAcc                                          <  1:1199382/149‑1 (MQ=255)
                                                                                                          gatcgatGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGACCGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGGAGATCACACAAccg                                         >  1:4098741/1‑149 (MQ=255)
                                                                                                          gatcgatGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGGAGATCACACAAccg                                         <  1:4572811/149‑1 (MQ=255)
                                                                                                           atcgatGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGGAGATCACACAAccgc                                        >  2:1681606/1‑149 (MQ=255)
                                                                                                           atcgatGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGGAGATCACACAAccgc                                        >  2:4499807/1‑149 (MQ=255)
                                                                                                            tcgatGACGCAGCGGCGCAGACGGCACGAGCGACCTACCCATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGACCGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGGAGATCACACAAccgcc                                       >  2:4947638/1‑149 (MQ=255)
                                                                                                            tcgatGACGCAGCGGCGCAGACGGCACGAGCGACCTACCCATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGGAGATCACACAAccgcc                                       >  2:2325555/1‑149 (MQ=255)
                                                                                                            tcgatGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGGAGATCACACAAccgcc                                       <  1:4783406/149‑1 (MQ=255)
                                                                                                            tcgatGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGGAGATCACACAAccgcc                                       >  2:3946691/1‑149 (MQ=255)
                                                                                                            tcgatGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGGAGATCACACAAccgcc                                       <  1:641463/149‑1 (MQ=255)
                                                                                                                 gACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGGAGATCACACAACCGCCGGaaa                                  <  2:5030462/149‑1 (MQ=255)
                                                                                                                       gcggcgCAGACGGCACGAGCGACCTACCCATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGGAGATCACACAACCGCCGGAAACCAGTg                            >  1:1208290/1‑149 (MQ=255)
                                                                                                                            gcAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGGAGATCACACAACCGCCGGAAACCAGTGAGTTa                       <  1:1983659/149‑1 (MQ=255)
                                                                                                                               gACGGCACGAGCGACCTACCCATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGGAGATCACACAACCGCCGGAAACCAGTGAGTTAAgg                    <  1:1750557/149‑1 (MQ=255)
                                                                                                                                  ggCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGGAGATCACACAACCGCCGGAAACCAGTGAGTTAAGGGTc                 >  2:4217864/1‑149 (MQ=255)
                                                                                                                                     aCGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGGAGATCACACAACCGCCGGAAACCAGTGAGTTAAGGGTCATc              <  2:4624550/149‑1 (MQ=255)
                                                                                                                                       gAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGACCGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGGAGATCACACAACCGCCGGAAACCAGTGAGTTAAGGGTCATccc            >  1:4990750/1‑149 (MQ=255)
                                                                                                                                       gAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGGAGATCACACAACCGCCGGAAACCAGTGAGTTAAGGGTCATccc            <  2:3414866/149‑1 (MQ=255)
                                                                                                                                            aCCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGGAGATCACACAACCGCCGGAAACCAGTGAGTTAAGGGTCATCCCGTGGc       <  2:4827963/149‑1 (MQ=255)
                                                                                                                                               tACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGGAGATCACACAACCGCCGGAAACCAGTGAGTTAAGGGTCATCCCGTGGCTAc    >  1:1570565/1‑149 (MQ=255)
                                                                                                                                                aCCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGGAGATCACACAACCGCCGGAAACCAGTGAGTTAAGGGTCATCCCGTGGCTAcc   >  1:553477/1‑149 (MQ=255)
                                                                                                                                                aCCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGGAGATCACACAACCGCCGGAAACCAGTGAGTTAAGGGTCATCCCGTGGCTAcc   >  2:2571396/1‑149 (MQ=255)
                                                                                                                                                 cccATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGCACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGGAGATCACACAACCGCCGGAAACCAGTGAGTTAAGGGTCATCCCGTGGCTACCg  >  1:4264254/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
ATTTCGCGCGTTACCAGCACGATGCCTTCTTCTGAACGATAGAAACGACGTGCATCTTCCTCTGCGTTTTCACCAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCCATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGGAGATCACACAACCGCCGGAAACCAGTGAGTTAAGGGTCATCCCGTGGCTACCG  >  CP009273/3561427‑3561720

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑