BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	3,664,370	G→T	6.7%	A473A (GCG→GCT)	treF →	cytoplasmic trehalase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	3,664,370	0	G	T	6.7%	1372.3 / 50.6	358	A473A (GCG→GCT)	treF	cytoplasmic trehalase
Reads supporting (aligned to +/- strand): ref base G (145/189); new base T (10/14); total (155/203)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

Only 100 of 366 total aligned reads displayed.

TCGGTATGGCGAACCATGAACAGGCCGATCGTCTGGCAAACGCCGTGCGCAGTCGGTTACTGACACCTGGCGGGATTCTGGCAAGCGAGTACGAAACCGGTGAACAGTGGGATAAACCCAACGGCTGGGCACCGTTACAATGGATGGCGATTCAGGGATTTAAAATGTACGGCGATGACCTTCTGGGTGATGAAATCGCGCGAAGCTGGCTGAAGACGGTGAATCAGTTCTATCTGGAACAGCACAAACTGATCGAAAAATACCATATTGCCGATGGTGTTCCCCGCGAAGGCGGCG  >  CP009273/3664222‑3664518

tCGGTATGGCGAACCATGAACAGGCCGATCGTCTGGCAAACGCCGTGCGCAGTCGGTTACTGACACCTGGCGGGATTCTGGCAAGCGAGTACGAAACCGGTGAACAGTGGGATAAACCCAACGGCTGGGCACCGTTACAATGGATGGCg                                                                                                                                                      >  1:2936436/1‑149 (MQ=255)

  ggTATGGCGAACCATGAACAGGCCGATCGTCTGGCAAACGCCGTGCGCAGTCGGTTACTGACACCTGGCGGGATTCTGGCAAGCGAGTACGAAACCGGTGAACAGTGGGATAAACCCAACGGCTGGGCACCGTTACAATGGATGGCGAt                                                                                                                                                    >  1:1191665/1‑149 (MQ=255)

  ggTATGGCGAACCATGAACAGGCCGATCGTCTGGCAAACGCCGTGCGCAGTCGGTTACTGACACCTGGCGGGATTCTGGCAAGCGAGTACGAAACCGGTGAACAGTGGGATAAACCCAACGGCTGGGCACCGTTACAATGGATGGCGAt                                                                                                                                                    <  1:842140/149‑1 (MQ=255)

   gTATGGCGAACCATGAACAGGCCGATCGTCTGGCAAACGCCGTGCGCAGTCGGTTACTGACACCTGGCGGGATTCTGGCAAGCGAGTACGAAACCGGTGAACAGTGGGATAAACCCAACGGCTGGGCACCGTTACAATGGATGGCGAtt                                                                                                                                                   >  1:1862191/1‑149 (MQ=255)

    tATGGCGAACCATGAACAGGCCGATCGTCTGGCAAACGCCGTGCGCAGTCGGTTACTGACACCTGGCGGGATTCTGGCAAGCGAGTACGAAACCGGTGAACAGTGGGATAAACCCAACGGCTGGGCACCGTTACACTGGATGGCGATTc                                                                                                                                                  >  2:2467139/1‑149 (MQ=255)

     aTGGCGAACCATGAACAGGCCGATCGTCTGGCAAACGCCGTGCGCAGTCGGTTACTGACACCTGGCGGGATTCTGGCAAGCGAGTACGAAACCGGTGAACAGTGGGATAAACCCAACGGCTGGGCACCGTTACAATGGATGGCGATTCa                                                                                                                                                 <  1:4106515/149‑1 (MQ=255)

       ggCGAACCATGAACAGGCCGATCGTCTGGCAAACGCCGTGCGCAGTCGGTTACTGACACCTGGCGGGATTCTGGCAAGCGAGTACGAAACCGGTGAACAGTGGGATAAACCCAACGGCTGGGCACCGTTACAATGGATGGCGATTCAgg                                                                                                                                               >  2:2255097/1‑149 (MQ=255)

       ggCGAACCATGAACAGGCCGATCGTCTGGCAAACGCCGTGCGCAGTCGGTTACTGACACCTGGCGGGATTCTGGCAAGCGAGTACGAAACCGGTGAACAGTGGGATAAACCCAACGGCTGGGCACCGTTACAATGGATGGCGATTCAgg                                                                                                                                               <  2:2395329/149‑1 (MQ=255)

         cGAACCATGAACAGGCCGATCGTCTGGCAAACGCCGTGCGCAGTCGGTTACTGACACCTGGCGGGATTCTGGCAAGCGAGTACGAAACCGGTGAACAGTGGGATAAACCCAACGGCTGGGCACCGTTACAATGGATGGCTATTCAGGGa                                                                                                                                             <  1:3490040/149‑1 (MQ=255)

         cGAACCATGAACAGGCCGATCGTCTGGCAAACGCCGTGCGCAGTCGGTTACTGACACCTGGCGGGATTCTGGCAAGCGAGTACGAAACCGGTGAACAGTGGGATAAACCCAACGGCTGGGCACCGTTACAAGTGATGGCGATTAAGGGa                                                                                                                                             >  1:2645206/1‑149 (MQ=255)

               aTGAACAGGCCGATCGTCTGGCAAACGCCGTGCGCAGTCGGTTACTGACACCTGGCGGGATTCTGGCAAGCGAGTACGAAACCGGTGAACAGTGGGATAAACCCAACGGCTGGGCACCGTTACAATGGATGGCGATTCAGGGATTTaaa                                                                                                                                       <  2:145899/149‑1 (MQ=255)

               aTGAACAGGCCGATCGTCTGGCAAACGCCGTGCGCAGTCGGTTACTGACACCTGGCGGGATTCTGGCAAGCGAGTACGAAACCGGTGAACAGTGGGATAAACCCAACGGCTGGGCACCGTTACAATGGATGGCGATTCAGGGATTTaaa                                                                                                                                       >  1:3065179/1‑149 (MQ=255)

               aTGAACAGGCCGATCGTCTGGCAAACGCCGTGCGCAGTCGGTTACTGACACCTGGCGGGATTCTGGCAAGCGAGTACGAAACCGGTGAACAGTGGGATAAACCCAACGGCTGGGCACCGTTACAATGGATGGCGATTCAGGGATTTaaa                                                                                                                                       >  1:4477326/1‑149 (MQ=255)

                tGAACAGGCCGATCGTCTGGCAAACGCCGTGCGCAGTCGGTTACTGACACCTGGCGGGATTCTGGCAAGCGAGTACGAAACCGGTGAACAGTGGGATAAACCCAACGGCTGGGCACCGTTACAATGGATGGCGATTCAGGGATTTaaaa                                                                                                                                      >  2:190452/1‑149 (MQ=255)

                tGAACAGGCCGATCGTCTGGCAAACGCCGTGCGCAGTCGGTTACTGACACCTGGCGGGATTCTGGCAAGCGAGTACGAAACCGGTGAACAGTGGGATAAACCCAACGGCTGGGCACCGTTACAATGGATGGCGATTCAGGGATTTaaaa                                                                                                                                      >  2:2815941/1‑149 (MQ=255)

                 gAACAGGCCGATCGTCTGGCAAACGCCGTGCGCAGTCGGTTACTGACACCTGGCGGGATTCTGGCAAGCGAGTACGAAACCGGTGAACAGTGGGATAAACCCAACGGCTGGGCACCGTTACAATGGATGGCGATTCAGGGATTTAAAAt                                                                                                                                     <  1:2852474/149‑1 (MQ=255)

                   agtggCCGATCGTCTGTCAATCGCCGTGCGCAGTCGGTTTCTGACACCTGGCGGGATTCTGGCAAGCGAGTACTAAACGGGTTTACTGTTGGATAAACCCAACGGCTGGGCTCCGTTACAATGGATGGCGATTCAGGGATTTAAAATGt                                                                                                                                   <  2:1784147/146‑1 (MQ=255)

                   aCAGGCCGATCGTCTGGCAAACGCCGTGCGCAGTCGGTTACTGACACCTGGCGGGATTCTGGCAAGCGAGTACGAAACCGGTGAACAGTGGGATAAACCCAACGGCTGGGCACCGTTACAATGGATGGCGATTCAGGGATTTAAAATGt                                                                                                                                   >  1:1669140/1‑149 (MQ=255)

                     aGGCCGATCGTCTGGCAAACGCCGTGCGCAGTCGGTTACTGACACCTGGCGGGATTCTGGCAAGCGAGTACGAAACCGGTGAACAGTGGGATAAACCCAACGGCTGGGCACCGTTACAATGGATGGCGATTCAGGGATTTAAAATGTAc                                                                                                                                 <  2:1086732/149‑1 (MQ=255)

                      ggCCGATCGTCTGGCAAACGCCGTGCGCAGTCGGTTACTGACACCTGGCGGGATTCTGGCAAGCGAGTACGAAACCGGTGAACAGTGGGATAAACCCAACGGCTGGGCACCGTTACAATGGATGGCGATTCAGGGATTTAAAATGTACg                                                                                                                                <  2:1511925/149‑1 (MQ=255)

                      ggCCGATCGTCTGGCAAACGCCGTGCGCAGTCGGTTACTGACACCTGGCGGGATTCTGGCAAGCGAGTACGAAACCGGTGAACAGTGGGATAAACCCAACGGCTGGGCACCGTTACAATGGATGGCGATTCAGGGATTTAAAATGTACg                                                                                                                                >  1:189245/1‑149 (MQ=255)

                       gCCGATCGTCTGGCAAACGCCGTGCGCAGTCGGTTACTGACACCTGGCGGGATTCTGGCAAGCGAGTACGAAACCGGTGAACAGTGGGATAAACCCAACGGCTGGGCACCGTTACAATGGATGGCGATTCAGGGATTTAAAATGTACgg                                                                                                                               >  1:1448125/1‑149 (MQ=255)

                        ccGATCGTCTGGCAAACGCCGTGCGCAGTCGGTTACTGACACCTGGCGGGATTCTGGCAAGCGAGTACGAAACCGGTGAACAGTGGGATAAACCCAACGGCTGGGCACCGTTACAATGGATGGCGATTCAGGGATTTAAAATGTACGGc                                                                                                                              <  2:1217026/149‑1 (MQ=255)

                        ccGATCGTCTGGCAAACGCCGTGCGCAGTCGGTTACTGACACCTGGCGGGATTCTGGCAAGCGAGTACGAAACCGGTGAACAGTGGGATAAACCCAACGGCTGGGCACCGTTACAATGGATGGCGATTCAGGGATTTAAAATGTACGGc                                                                                                                              >  2:4278308/1‑149 (MQ=255)

                          gATCGTCTGGCAAACGCCGTGCGCAGTCGGTTACTGACACCTGGCGGGATTCTGGCAAGCGAGTACGAAACCGGTGAACAGTGGGATAAACCCAACGGCTGGGCACCGTTACAATGGATGGCGATTCAGGGATTTAAAATGTACGGCGa                                                                                                                            >  2:5137665/1‑149 (MQ=255)

                          gATCGTCTGGCAAACGCCGTGCGCAGTCGGTTACTGACACCTGGCGGGATTCTGGCAAGCGAGTACGAAACCGGTGAACAGTGGGATAAACCCAACGGCTGGGCACCGTTACAATGGATGGCGATTCAGGGATTTAAAATGTACGGCGa                                                                                                                            >  1:2703826/1‑149 (MQ=255)

                           aTCGTCTGGCAAACGCCGTGCGCAGTCGGTTACTGACACCTGGCGGGATTCTGGCAAGCGAGTACGAAACCGGTGAACAGTGGGATAAACCCAACGGCTGGGCACCGTTACAATGGATGGCGATTCAGGGATTTAAAATGTACGGCGAt                                                                                                                           <  1:741527/149‑1 (MQ=255)

                            tCGTCTGGCAAACGCCGTGCGCAGTCGGTTACTGACACCTGGCGGGATTCTGGCAAGCGAGTACGAAACCGGTGAACAGTGGGATAAACCCAACGGCTGGGCACCGTTACAATGGATGGCGATTCAGGGATTTAAAATGTACGGCGATg                                                                                                                          >  2:4641765/1‑149 (MQ=255)

                              gTCTGGCAAACGCCGTGCGCAGTCGGTTACTGACACCTGGCGGGATTCTGGCAAGCGAGTACGAAACCGGTGAACAGTGGGATAAACCCAACGGCTGGGCACCGTTACAATGGATGGCGATTCAGGGATTTAAAATGTACGGCGATGAc                                                                                                                        >  2:658653/1‑149 (MQ=255)

                               tCTGGCAAACGCCGTGCGCAGTCGGTTACTGACACCTGGCGGGATTCTGGCAAGCGAGTACGAAACCGGTGAACAGTGGGATAAACCCAACGGCTGGGCACCGTTACAATGGATGGCGATTCAGGGATTTAAAATGTACGGCGATGAcc                                                                                                                       >  1:3201577/1‑149 (MQ=255)

                                 tGGCAAACGCCGTGCGCAGTCGGTTACTGACACCTGGCGGGATTCTGGCAAGCGAGTACGAAACCGGTGAACAGTGGGATAAACCCAACGGCTGGGCACCGTTACAATGGATGGCGATTCAGGGATTTAAAATGTACGGCGATGACCtt                                                                                                                     >  2:2176988/1‑149 (MQ=255)

                                   gCAAACGCCGTGCGCAGTCGGTTACTGACACCTGGCGGGATTCTGGCAAGCGAGTACGAAAACGGTGAACAGTGGGATAAACCCAACGGCTGGGCACCGTTACCATGGATGGCGATTCAGGGATTTAAAATGTACGGCGATGACCTGCt                                                                                                                   >  1:2028079/1‑149 (MQ=255)

                                     aaaCGCCGTGCGCAGTCGGTTACTGACACCTGGCGGGATTCTGGCAAGCGAGTACGAAACCGGTGAACAGTGGGATAAGCCCAACGGCTGGGCACCGTTACAATGGATGGCGATTCAGGGATTTAAAATGTACGGCGATGACCTTCTgg                                                                                                                 <  2:4718294/149‑1 (MQ=255)

                                      aaCGCCGTGCGCAGTCGGTTACTGACACCTGGCGGGATTCTGGCAAGCGAGTACGAAACCGGTGAACAGTGGGATAAACCCAACGGCTGGGCACCGTTACAATGGATGGCGATTCAGGGATTTAAAATGTACGGCGATGACCTTCTggg                                                                                                                >  2:4862121/1‑149 (MQ=255)

                                      aaCGCCGTGCGCAGTCGGTTACTGACACCTGGCGGGATTCTGGCAAGCGAGTACGAAACCGGTGAACAGTGGGATAAACCCAACGGCTGGGCACCGTTACAATGGATGGCGATTCAGGGATTTAAAATGTACGGCGATGACCTTCTggg                                                                                                                <  1:4852836/149‑1 (MQ=255)

                                       aCGCCGTGCGCAGTCGGTTACTGACACCTGGCGGGATTCTGGCAAGCGAGTACGAAACCGGTGAACAGTGGGATAAACCCAACGGCTGGGCACCGTTACAATGGATGGCGATTCAGGGATTTAAAATGTACGGCGATGACCTTCTGGGt                                                                                                               <  2:1685649/149‑1 (MQ=255)

                                        cGCCGTGCGCAGTCGGTTACTGACACCTGGCGGGATTCTGGCAAGCGAGTACGAAACCGGTGAACAGTGGGATAAACCCAACGGCTGGGCACCGTTACAATGGATGGCGATTCAGGGATTTAAAATGTACGGCGATGACCTTCTGGGtg                                                                                                              <  2:1855026/149‑1 (MQ=255)

                                          ccGTGCGCAGTCGGTTACTGACACCTGGCGGGATTCTGGCAAGCGAGTACGAAACCGGTGAACAGTGGGATAAACCCAACGGCTGGGCACCGTTACAATGGATGGCGATTCAGGGATTTAAAATGTACGGCGATGACCTTCTGGGtgat                                                                                                            <  2:4319500/149‑1 (MQ=255)

                                            gTGCGCAGTCGGTTACTGACACCTGGCGGGATTCTGGCAAGCGAGTACGAAACCGGTGAACAGTGGGATAAACCCAACGGCTGGGCACCGTTACAATGGATGGCGATTCAGGGATTTAAAATGTACGGCGATGACCTTCTGGGtgatga                                                                                                          >  1:3813458/1‑149 (MQ=255)

                                                gcAGTCGGTTACTGACACCTGGCGGGATTCTGGCAAGCGAGTACGAAACCGGTGAACAGTGGGATAAACCCAACGGCTGGGCACCGTTACAATGGATGGCGATTCAGGGATTTAAAATGTACGGCGATGACCTTCTGGGTGATGAAATc                                                                                                      <  1:4813808/149‑1 (MQ=255)

                                                         tACTGACACCTGGCGGGATTCTGGCAAGCGAGTACGAAACCGGTGAACAGTGGGATAAACCCAACGGCTGGGCACCGTTACAATGGATGGCGATTCAGGGATTTAAAATGTACGGCGATGACCTTCTGGGTGATGAAATCGCGCGAAgc                                                                                             <  1:4051899/149‑1 (MQ=255)

                                                         tACTGACACCTGGCGGGATTCTGGCAAGCGAGTACGAAACCGGTGAACAGTGGGATAAACCCAACGGCTGGGCACCGTTACAATGGATGGCGATTCAGGGATTTAAAATGTACGGCGATGACCTTCTGGGTGATGAAATCGCGCGAAgc                                                                                             >  2:2448668/1‑149 (MQ=255)

                                                         tACTGACACCTGGAGGGATTCTGGCAAGCGAGTACGAAACCGGTGAACAGTGGGATAAACCCAACGGCTGGGCACCGTTACAATGGATGGCGATTCAGGGATTTAAAATGTACGGCGATGACCTTCTGGGTGATGAAATCGCGCGAAgc                                                                                             >  2:590405/1‑149 (MQ=255)

                                                            tGACACCTGGCGGGATTCTGGCAAGCGAGTACGAAACCGGTGAACAGTGGGATAAACCCAACGGCTGGGCACCGTTACAATGGATGGCGATTCAGGGATTTAAAATGTACGGCGATGACCTTCTGGGTGATGAAATCGCGCGAAgctgg                                                                                          <  1:4674540/149‑1 (MQ=255)

                                                             gACACCTGGCGGGATTCTGGCAAGCGAGTACGAAACCGGTGAACAGTGGGATAAACCCAACGGCTGGGCACCGTTACAATGGATGGCGATTCAGGGATTTAAAATGTACGGCGATGACCTTCTGGGTGATGAAATCGCGCGAAgctggc                                                                                         >  2:4461265/1‑149 (MQ=255)

                                                             gACACCTGGCGGGATTCTGGCAAGCGAGTACGAAACCGGTGAACAGTGGGATAAACCCAACGGCTGGGCACCGTTACAATGGATGGCGATTCAGGGATTTAAAATGTACGGCGATGACCTTCTGGGTGATGAAATCGCGCGAAgctggc                                                                                         >  2:3836598/1‑149 (MQ=255)

                                                                 ccTGGCGGGATTCTGGCAAGCGAGTACGAAACCGGTGAACAGTGGGATAAACCCAACGGCTGGGCACCGTTACAATGGATGGCGATTCAGGGATTTAAAATGTACGGCGATGACCTTCTGGGTGATGAAATCGCGCGAAGCTGGCTGaa                                                                                     <  1:4388838/149‑1 (MQ=255)

                                                                  cTGGCGGGATTCTGGCAAGCGAGTACGAAACCGGTGAACAGTGGGATAAACCCAACGGCTGGGCACCGTTACAATGGATGGCGATTCAGGGATTTAAAATGTACGGCGATGACCTTCTGGGTGATGAAATCGCGCGAAGCTGGCTGAAg                                                                                    <  1:4502887/149‑1 (MQ=255)

                                                                    ggCGGGATTCTGGCAAGCGAGTACGAAACCGGTGAACAGTGGGATAAACCCAACGGCTGGGCACCGTTACAATGGATGGCGATTCAGGGATTTAAAATGTACGGCGATGACCTTCTGGGTGATGAAATCGCGCGAAGCTGGCTGAAGAc                                                                                  >  2:4898085/1‑149 (MQ=255)

                                                                       gggATTCTGGCAAGCGAGTACGAAACCGGTGAACAGTGGGATAAACCCAACGGCTGGGCACCGTTACAATGGATGGCGATTCAGGGATTTAAAATGTACGGCGATGACCTTCTGGGTGATGAAATCGCGCGAAGCTGGCTGAAGACGGt                                                                               <  2:3063312/149‑1 (MQ=255)

                                                                           ttCTGGCAAGCGAGTACGAAACCGGTGAACAGTGGGATAAACCCAACGGCTGGGCACCGTTACAATGGATGGCGATTCAGGGATTTAAAATGTACGGCGATGACCTTCTGGGTGATGAAATCGCGCGAAGCTGGCTGAAGACGGTGAAt                                                                           <  2:3514718/149‑1 (MQ=255)

                                                                            tCTGGCAAGCGAGTACGAAACCGGTGAACAGTGGGATAAACCCAACGGCTGGGCACCGTTACAATGGATGGCGATTCAGGGATTTAAAATGTACGGCGATGACCTTCTGGGTGATGAAATCGCGCGAAGCTGGCTGAAGACGGTGAATc                                                                          <  1:1501113/149‑1 (MQ=255)

                                                                             cTGGCAAGCGAGTACGAAACCGGTGAACAGTGGGATAAACCCAACGGCTGGGCACCGTTACAATGGATGGCGATTCAGGGATTTAAAATGTACGGCGATGACCTTCTGGGTGATGAAATCGCGCGAAGCTGGCTGAAGACGGTGAATCa                                                                         >  1:2101213/1‑149 (MQ=255)

                                                                             cTGGCAAGCGAGTACGAAACCGGTGAACAGTGGGATAAACCCAACGGCTGGGCACCGTTACAATGGATGGCGATTCAGGGATTTAAAATGTACGGCGATGACCTTCTGGGTGATGAAATCGCGCGAAGCTGGCTGAAGACGGTGAATCa                                                                         <  2:5034701/149‑1 (MQ=255)

                                                                               ggCAAGCGAGTACGAAACCGGTGAACAGTGGGATAAACCCAACGGCTGGGCACCGTTACAATGGATGGCGATTCAGGGATTTAAAATGTACGGCGATGACCTTCTGGGTGATGAAATCGCGCGAAGCTGGCTGAAGACGGTGAATCAGt                                                                       <  2:439805/149‑1 (MQ=255)

                                                                                gCAAGCGAGTACGAAACCGGTGAACAGTGGGATAAACCCAACGGCTGGGCACCGTTACAATGGATGGCGATTCAGGGATTTAAAATGTTCGGCGATGACCTTCTGGGTGATGAAATCGCGCGAAGCTGGCTGAAGACGGTGAATCAGtt                                                                      <  1:2411105/149‑1 (MQ=255)

                                                                                 cAAGCGAGTACGAAACCGGTGAACAGTGGGATAAACCCAACGGCTGGGCACCGTTACAATGGATGGCGATTCAGGGATTTAAAATGTACGGCGATGACCTTCTGGGTGATGAAATCGCGCGAAGCTGGCTGAAGACGGTGAATCAGTTc                                                                     <  2:4572643/149‑1 (MQ=255)

                                                                                  aaGCGAGTACGAAACCGGTGAACAGTGGGATAAACCCAACGGCTGGGCACCGTTACAATGGATGGCTATTCAGGGATTTAAAATGTACGGCGATGACCTTCTGGGTGATGAAATCGCGCGAAGCTGGCTGAAGACGGTGAATCAGTTCt                                                                    <  2:4007232/149‑1 (MQ=255)

                                                                                  aaGCGAGTACGAAACCGGTGAACAGTGGGATAAACCCAACGGCTGGGCACCGTTACAATGGATGGCGATTCAGGGATTTAAAATGTACGGCGATGACCTTCTGGGTGATGAAATCGCGCGAAGCTGGCTGAAGACGGTGAATCAGTTCt                                                                    >  2:304277/1‑149 (MQ=255)

                                                                                    gCGAGTACGAAACCGGTGAACAGTGGGATAAACCCAACGGCTGGGCACCGTTACAATGGATGGCGATTCAGGGATTTAAAATGTACGGCGATGACCTTCTGGGTGATGAAATCGCGCGAAGCTGGCTGAAGACGGTGAATCAGTTCTAt                                                                  <  1:134880/149‑1 (MQ=255)

                                                                                    gCGAGTACGAAACCGGTGAACAGTGGGATAAACCCAACGGCTGGGCACCGTTACAATGGATGGCGATTCAGGGATTTAAAATGTACGGCGATGACCTTCTGGGTGATGAAATCGCGCGAAGCTGGCTGAAGACGGTGAATCAGTTCTAt                                                                  >  1:224856/1‑149 (MQ=255)

                                                                                       aGTACGAAACCGGTGAACAGTGGGATAAACCCAACGGCTGGGCACCGTTACAATGGATGGCGATTCAGGGATTTAAAATGTACGGCGATGACCTTCTGGGTGATGAAATCGCGCGAAGCTGGCTGAAGACGGTGAATCAGTTCTATCTg                                                               >  1:1230886/1‑149 (MQ=255)

                                                                                              aaCCGGTGAACAGTGGGATAAACCCAACGGCTGGGCACCGTTACAATGGATGGCTATTCAGGGATTTAAAATGTACGGCGATGACCTTCTGGGTGATGAAATCGCGCGAAGCTGGCTGAAGACGGTGAATCAGTTCTATCTGGAACAGc                                                        <  1:1022524/149‑1 (MQ=255)

                                                                                                 cGGTGAACAGTGGGATAAACCCAACGGCTGGGCACCGTTACAATGGATGGCGATTCAGGGATTTAAAATGTACGGCGATGACCTTCTGGGTGATGAAATCGCGCGAAGCTGGCTGAAGACGGTGAATCAGTTCTATCTGGAACAGcaca                                                     <  1:4254009/149‑1 (MQ=255)

                                                                                                   gTGAACAGTGGGATAAACCCAACGGCTGGGCACCGTTACAATGGATGGCGATTCAGGGATTTAAAATGTACGGCGATGACCTTCTGGGTGATGAAATCGCGCGAAGCTGGCTGAAGACGGTGAATCAGTTCTATCTGGAACAGCACaaa                                                   >  1:5122069/1‑149 (MQ=255)

                                                                                                    tGAACAGTGGTATAAACCCAACGGCTGGGCACCGTTACAATGGATGGCGATTCAGTGATTTAAAATGTACGGCGATGACCTTCTGGGTGATGAAATCGCGCGAAGCTGGCTGAAGACGGTGAATCAGTTCTATCTGGAACAGCACAAAc                                                  <  2:3152161/149‑1 (MQ=255)

                                                                                                    tGAACAGTGGGATAAACCCAACGGCTGGGCACCGTTACAATGGATGGCGATTCAGGGATTTAAAATGTACGGCGATGACCTTCTGGGTGATGAAATCGCGCGAAGCTGGCTGAAGACGGTGAATCAGTTCTATCTGGAACAGCACAAAc                                                  <  2:1002692/149‑1 (MQ=255)

                                                                                                    tGAACAGGGGGATAAACCCAACGGCTGGGCACCGTTACAATGGATGGCGATTCAGGGATTTAAAATGTACGGCGATGACCTTCTGGGTGATGAAATCGCGCGAAGCTGGCTGAAGACGGTGAATCAGTTCTATCTGGAACAGCACAAAc                                                  <  1:3108114/149‑1 (MQ=255)

                                                                                                       aCAGTGGGATAAACCCAACGGCTGGGCACCGTTACAATGGATGGCGATTCAGGGATTTAAAATGTACGGCGATGACCTTCTGGGTGATGAAATCGCGCGAAGCTGGCTGAAGACGGTGAATCAGTTCTATCTGGAACAGCACAAACTGa                                               <  1:1811585/149‑1 (MQ=255)

                                                                                                         aGTGGGATAAACCCAACGGCTGGGCACCGTTACAATGGATGGCGATTCAGGGATTTAAAATGTACGGCGATGACCTTCTGGGTGATGAAATCGCGCGAAGCTGGCTGAAGACGGTGAATCAGTCCTATCTGGAACAGCACAAACTGATc                                             <  1:935935/149‑1 (MQ=255)

                                                                                                           tGGGATAAACCCAACGGCTGGGCACCGTTACAATGGATGGCGATTCAGGGATTTAAAATGTACGGCGATGACCTTCTGGGTGATGAAATCGCGCGAAGCTGGCTGAAGACGGTGAATCAGTTCTATCTGGAACAGCACAAACTGATCGa                                           >  1:4230328/1‑149 (MQ=255)

                                                                                                           tGGGATAAACCCAACGGCTGGGCACCGTTACAATGGATGGCGATTCAGGGATTTAAAATGTACGGCGATGACCTTCTGGGTGATGAAATCGCGCGAAGCTGGCTGAAGACGGTGAATCAGTTCTATCTGGAACAGCACAAACTGATCGa                                           <  1:604407/149‑1 (MQ=255)

                                                                                                            gggATAAACCCAACGGCTGGGCACCGTTACAATGGATGGCGATTCAGGGATTTAAAATGTACGGCGATGACCTTCTGGGTGATGAAATCGCGCGAAGCTGGCTGAAGACGGTGAATCAGTTCTATCTGGAACAGCACAAACTGATCGaa                                          <  1:4878255/149‑1 (MQ=255)

                                                                                                                tAAACCCAACGGCTGGGCACCGTTACAATGGATGGCGATTCAGGGATTTAAAATGTACGGCGATGACCTTCTGGGTGATGAAATCGCGCGAAGCTGGCTGAAGACGGTGAATCAGTTCTATCTGGAACAGCACAAACTGATCGAAAAAt                                      <  2:4454335/149‑1 (MQ=255)

                                                                                                                 aaaCCCAACGGCTGGGCACCGTTACAATGGATGGCGATTCAGGGATTTAAAATGTACGGCGATGACCTTCTGGGTGATGAAATCGCGCGAAGCTGGCTGAAGACGGTGAATCAGTTCTATCTGGAACAGCACAAACTGATCGAAAAATa                                     >  1:3943372/1‑149 (MQ=255)

                                                                                                                  aaCCCAACGGCTGGGCACCGTTACAATGGATGGCTATTCAGGGATTTAAAATGTACGGCGATGACCTTCTGGGTGATGAAATCGCGCGAAGCTGGCTGAAGACGGTGAATCAGTTCTATCTGGAACAGCACAAACTGATCGAAAAATAc                                    >  2:2618942/1‑149 (MQ=255)

                                                                                                                  aaCCCAACGGCTGGGCACCGTTACAATGGATGGCGATTCAGGGATTTAAAATGTACGGCGATGACCTTCTGGGTGATGAAATCGCGCGAAGCTGGCTGAAGACGGTGAATCAGTTCTATCTGGAACAGCACAAACTGATCGAAAAATAc                                    <  2:2254836/149‑1 (MQ=255)

                                                                                                                  aaCCCAACGGCTGGGCACCGTTACAATGGATGGCGATTCAGGGATTTAAAATGTACGGCGATGACCTTCTGGGTGATGAAATCGCGCGAAGCTGGCTGAAGACGGTGAATCAGTTCTATCTGGAACAGCACAAACTGATCGAAAAATAc                                    <  2:1571840/149‑1 (MQ=255)

                                                                                                                    cccAACGGCTGGGCACCGTTACAATGGATGGCGATTCAGGGATTTAAAATGTACGGCGATGACCTTCTGGGTGATGAAATCGCGCGAAGCTGGCTGAAGACGGTGAATCAGTTCTATCTGGAACAGCACAAACTGATCGAAAAATACCa                                  <  2:4001307/149‑1 (MQ=255)

                                                                                                                    cccAACGGCTGGGCACCGTTACAATGGATGGCGATTCAGGGATTTAAAATGTACGGCGATGACCTTCTGGGTGATGAAATCGCGCGAAGCTGGCTGAAGACGGTGAATCAGTTCTATCTGGAACAGCACAAACTGATCGAAAAATACCa                                  <  2:832174/149‑1 (MQ=255)

                                                                                                                    cccAACGGCTGGGCACCGTTACAATGGATGGCGATTCAGGGATTTAAAATGTACGGCGATGACCTTCTGGGTGATGAAATCGCGCGAAGCTGGCTGAAGACGGTGAATCAGTTCTATCTGGAACAGCACAAACTGATCGAAAAATACCa                                  <  2:1382537/149‑1 (MQ=255)

                                                                                                                     ccAACGGCTGGGCACCGTTACAATGGATGGCGATTCAGGGATTTAAAATGTACGGCGATGACCTTCTGGGTGATGAAATCGCGCGAAGCTGGCTGAAGACGGTGAATCAGTTCTATCTGGAACAGCACAAACTGATCGAAAAATACCat                                 >  2:3335681/1‑149 (MQ=255)

                                                                                                                      cAACGGCTGGGCACCGTTACAATGGATGGCGATTCAGGGATTTAAAATGTACGGCGATGACCTTCTGGGTGATGAAATCGCGCGAAGCTGGCTGAAGACGGTGAATCAGTTCTATCTGGAACAGCACAAACTGATCGAAAAATACCata                                <  1:3415298/149‑1 (MQ=255)

                                                                                                                       aaCGGCTGGGCACCGTTACAATGGATGGCGATTCAGGGATTTAAAATGTACGGCGATGACCTTCTGGGTGATGAAATCGCGCGAAGCTGGCTGAAGACGGTGAATCAGTTCTATCTGGAACAGCACAAACTGATCGAAAAATACCatat                               <  1:3559028/149‑1 (MQ=255)

                                                                                                                       aaCGGCTGGGCACCGTTACAATGGATGGCGATTCAGGGATTTAAAATGTACGGCGATGACCTTCTGGGTGATGAAATCGCGCGAAGCTGGCTGAAGACGGTGAATCAGTTCTATCTGGAACAGCACAAACTGATCGAAAAATACCatat                               >  1:3626694/1‑149 (MQ=255)

                                                                                                                        aCGGCTGGGCACCGTTACAATGGATGGCGATTCAGGGATTTAAAATGTACGGCGATGACCTTCTGGGTGATGAAATCGCGCGAAGCTGGCTGAAGACGGTGAATCAGTTCTATCTGGAACAGCACAAACTGATCGAAAAATACCATAtt                              <  2:1804916/149‑1 (MQ=255)

                                                                                                                        aCGGCTGGGCACCGTTACAATGGATGGCGATTCAGGGATTTAAAATGTACGGCGATGACCTTCTGGGTGATGAAATCGCGCGAAGCTGGCTGAAGACGGTGAATCAGTTCTATCTGGAACAGCACAAACTGATCGAAAAATACCATAtt                              >  1:4989113/1‑149 (MQ=255)

                                                                                                                               ggCACCGTTACAATGGATGGCGATTCAGGGATTTAAAATGTACGGCGATGACCTTCTGGGTGATGAAATCGCGCGAAGCTGGCTGAAGACGGTGAATCAGTTCTATCTGGAACAGCACAAACTGATCGAAAAATACCATATTGCCGATg                       <  2:2101213/149‑1 (MQ=255)

                                                                                                                                 cACCGTTACAATGGATGGCGATTCAGGGATTTAAAATGTACGGCGATGACCTTCTGGGTGATGAAATCGCGCGAAGCTGGCTGAAGACGGTGAATCAGTTCTATCTGGAACAGCACAAACTGATCGAAAAATACCATATTGCCGATGgt                     <  1:2543246/149‑1 (MQ=255)

                                                                                                                                   ccGTTACAATGGATGGCGATTCAGGGATTTAAAATGTACGGCGATGACCTTCTGGGTGATGAAATCGCGCGAAGCTGGCTGAAGACGGTGAATCAGTTCTATCTGGAACAGCACAAACTGATCGAAAAATACCATATTGCCGATGgtgt                   <  2:3743379/149‑1 (MQ=255)

                                                                                                                                        aCAATGGATGGCGATTCAGGGATTTAAAATGTACGGCGATGACCTTCTGGGTGATGAAATCGCGCGAAGCTGGCTGAAGACGGTGAATCAGTTCTATCTGGAACAGCACAAACTGATCGAAAAATACCATATTGCCGATGGTGTTcccc              <  2:1987712/149‑1 (MQ=255)

                                                                                                                                        aCAATGGATGGCGATTCAGGGATTTAAAATGTACGGCGATGACCTTCTGGGTGATGAAATCGCGCGAAGCTGGCTGAAGACGGTGAATCAGTTCTATCTGGAACAGCACAAACTGATCGAAAAATACCATATTGCCGATGGTGTTcccc              <  2:28609/149‑1 (MQ=255)

                                                                                                                                          aaTGGATGGCGATTCAGGGATTTAAAATGTACGGCGATGACCTTCTGGGTGATGAAATCGCGCGAAGCTGGCTGAAGACGGTGAATCAGTTCTATCTGGAACAGCACAAACTGATCGAAAAATACCAAATTGCCGATGGTGTTCCCcgc            >  2:205115/1‑149 (MQ=255)

                                                                                                                                           atggatggCGATTCAGGGATTTAAAATGTACGGCGATGACCTTCTGGGTGATGAAATCGCGCGAAGCTGGCTGAAGACGGTGAATCAGTTCTATCTGGAACAGCACAAACTGATCGAAAAATACCATATTGCCGATGGTGTTCCCcgcg           >  1:5223573/1‑149 (MQ=255)

                                                                                                                                            tggatggCGATTCAGGGATTTAAAATGTACGGCGATGACCTTCTGGGTGATGAAATCGCGCGAAGCTGGCTGAAGACGGTGAATCAGTTCTATCTGGAACAGCACAAACTGATCGAAAAATACCATATTGCCGATGGTGTTCCCCGCGa          >  1:2394596/1‑149 (MQ=255)

                                                                                                                                             ggatggCGATTCAGGGATTTAAAATGTACGGCGATGACCTTCTGGGTGATGAAATCGCGCGAAGCTGGCTGAAGACGGTGAATCAGTTCTATCTGGAACAGCACAAACTGATCGAAAAATACCATATTGCCGATGGTGTTCCCCGCGaa         <  2:3272791/149‑1 (MQ=255)

                                                                                                                                              gatggCGATTCAGGGATTTAAAATGTACGGCGATGACCTTCTGGGTGATGAAATCGCGCGAAGCTGGCTGAAGACGGTGAATCAGTTCTATCTGGAACAGCACAAACTGATCGAAAAATGCCATATTGCCGATGGTGTTCCCCGCGAAg        >  1:1098168/1‑149 (MQ=255)

                                                                                                                                                tggCGATTCAGGGATTTAAAATGTACGGCGATGACCTTCTGGGTGATGAAATCGCGCGAAGCTGGCTGAAGACGGTGAATCAGTTCTATCTGGAACAGCACAAACTGATCGAAAAATACCATATTGCCGATGGTGTTCCCCGCGAAggc      >  1:3282184/1‑149 (MQ=255)

                                                                                                                                                tggCGATTCAGGGATTTAAAATGTACGGCGATGACCTTCTGGGTGATGAAATCGCGCGAAGCTGGCTGAAGACGGTGAATCAGTTCTATCTGGAACAGCACAAACTGATCGAAAAATACCATATTGCCGATGGTGTTCCCCGCGAAggc      >  1:2466922/1‑149 (MQ=255)

                                                                                                                                                   cGATTCAGGGATTTAAAATGTACGGCGATGACCTTCTGGGTGATGAAATCGCGCGAAGCTGGCTGAAGACGGTGAATCAGTTCTATCTGGAACAGCACAAACTGATCGAAAAATACCATATTGCCGATGGTGTTCCCCGCGAAggcggc   <  1:3704047/149‑1 (MQ=255)

TCGGTATGGCGAACCATGAACAGGCCGATCGTCTGGCAAACGCCGTGCGCAGTCGGTTACTGACACCTGGCGGGATTCTGGCAAGCGAGTACGAAACCGGTGAACAGTGGGATAAACCCAACGGCTGGGCACCGTTACAATGGATGGCGATTCAGGGATTTAAAATGTACGGCGATGACCTTCTGGGTGATGAAATCGCGCGAAGCTGGCTGAAGACGGTGAATCAGTTCTATCTGGAACAGCACAAACTGATCGAAAAATACCATATTGCCGATGGTGTTCCCCGCGAAGGCGGCG  >  CP009273/3664222‑3664518

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`