BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	3,822,340	A→C	14.8%	Q12H (CAA→CAC)	xanP →	xanthine permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	3,822,340	0	A	C	14.8%	537.5 / 19.1	280	Q12H (CAA→CAC)	xanP	xanthine permease
Reads supporting (aligned to +/- strand): ref base A (144/87); new base C (15/34); total (159/121)
Fisher's exact test for biased strand distribution p-value = 5.93e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 286 total aligned reads displayed.

TATAAAAAAGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTGCCAGGCGCTGG  >  CP009273/3822192‑3822488

tataAAAAAGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCaa                                                                                                                                                      >  1:3497559/1‑149 (MQ=255)

tataAAAAAGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCaa                                                                                                                                                      <  1:4870619/149‑1 (MQ=255)

  taAAAAAGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAAcc                                                                                                                                                    >  1:3332401/1‑149 (MQ=255)

   aaaaaaGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCCAccc                                                                                                                                                   >  1:4576988/1‑148 (MQ=255)

   aaaaaaGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCg                                                                                                                                                   >  1:3884112/1‑149 (MQ=255)

    aaaaaGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCgg                                                                                                                                                  >  2:4019935/1‑149 (MQ=255)

     aaaaGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGt                                                                                                                                                 <  2:1353827/149‑1 (MQ=255)

     aaaaGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGt                                                                                                                                                 >  2:3754080/1‑149 (MQ=255)

     aaaaGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTAGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGt                                                                                                                                                 >  2:1076463/1‑149 (MQ=255)

             aTTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCATTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGAGTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGAGCAACCCGTTGCGgaga                                                                                                                                         >  1:3263270/1‑149 (MQ=255)

              tttATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCCCCCGGTTGCGCCGAc                                                                                                                                        >  2:2879373/1‑149 (MQ=255)

              tttATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGAc                                                                                                                                        >  2:3486614/1‑149 (MQ=255)

               ttATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTGTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACt                                                                                                                                       >  2:854622/1‑149 (MQ=255)

                 aTCTCATTTAAATATTTAGCTGAGGATGTGGCAAACGTTTGCTTTTCTCTGTCGGTCAGCTCAACTGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGGCAGAAAATGCGCAACCGGTTGCGCAGACTCa                                                                                                                                     <  2:1235541/149‑1 (MQ=255)

                 aTCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCa                                                                                                                                     >  1:3373900/1‑149 (MQ=255)

                 aTCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAAACCGTTTCGCAGACTCa                                                                                                                                     >  1:4101086/1‑149 (MQ=255)

                  tctcATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCaa                                                                                                                                    >  2:2346723/1‑149 (MQ=255)

                   ctaaTTTAAATTTTTAGTTGTGGTTGTGGCAAAGGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACAACGGTATTGTTTTCGTTGTCTGTTTCCACCCTCTAGTCATAAAATGCGCAACCGGTTGCGCAGACTCaaa                                                                                                                                   <  2:112587/146‑1 (MQ=255)

                    tcATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCCGTTGCGCAGACACaaaa                                                                                                                                  >  1:1711162/1‑149 (MQ=255)

                    tcATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTGTCTCTTTCGGGCAGCTAAAATGCCCGCTTTGCTACCACGGTATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCaaaa                                                                                                                                  <  2:5002143/149‑1 (MQ=255)

                       tttAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCCACCCGTTGAGCAGAAACaaaaaaa                                                                                                                               >  1:3098935/1‑146 (MQ=255)

                         tAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCg                                                                                                                             >  2:5187562/1‑149 (MQ=255)

                           aaTATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGaa                                                                                                                           >  2:4619651/1‑149 (MQ=255)

                             tttttAGTTGTGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGTTTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACGGGTTGCGCAGACTCAAAACAGCGAACt                                                                                                                         <  2:636857/147‑1 (MQ=255)

                             tatTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCCGTTGCGCAGACTCAAAAAAGCGAAca                                                                                                                         >  1:219460/1‑148 (MQ=255)

                              atTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCCACCGGTTGCGCAGACTCAAAACAGCGAACTg                                                                                                                        >  2:1819189/1‑149 (MQ=255)

                               tttAGATTAGGATGTGGCAAACGTTTGCTTAAAACATTCGGACAGCTAAAATGCCCGCTTTGCTACCACGGGATTGATTTCGAAGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAAACGCTTTCGCAGACACAAAACAGCGAAcata                                                                                                                       >  2:2596767/1‑146 (MQ=255)

                               tttAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGa                                                                                                                       >  2:3564863/1‑149 (MQ=255)

                                ttAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCCGTTGCGCAGAATCAAAAAAACGAACAGaa                                                                                                                      >  1:968522/1‑148 (MQ=255)

                                   gATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTa                                                                                                                   >  2:2327974/1‑149 (MQ=255)

                                    aTGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTAc                                                                                                                  >  2:4953116/1‑149 (MQ=255)

                                    aTGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCACACTCAAAACAGCGAACTGATTTAc                                                                                                                  >  1:848308/1‑149 (MQ=255)

                                     tgtggttgtgGAAAACGTTTTCTTTTCTCTTTCGGGAAGATAAACGGCCCGCTGTGCTACCACGGTTTTTTTTTCGATGTCTGTTTCCACCCTCGATTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTAcc                                                                                                                 <  1:3442520/143‑1 (MQ=255)

                                     tGAGGTTGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTAcc                                                                                                                 <  2:4866982/149‑1 (MQ=255)

                                     tGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTAcc                                                                                                                 >  2:1585272/1‑149 (MQ=255)

                                       aGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAAAAGCGAACAGATTTAACGt                                                                                                               >  1:2095243/1‑149 (MQ=255)

                                        ggATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCCGACTCAAAACAGCGAACTGATTTAACGTc                                                                                                              >  1:3720276/1‑149 (MQ=255)

                                        ggATGTGGCAAACGTTGGCTTGTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGATACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAAGGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTc                                                                                                              <  2:2967067/149‑1 (MQ=255)

                                               gCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGa                                                                                                       >  2:1705682/1‑149 (MQ=255)

                                               gCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCCGTTGCGCAGACTCAAAAAAGCGCACGGATTTACCGTCGTGTAGa                                                                                                       >  1:1104562/1‑149 (MQ=255)

                                                cAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCCGGTGCGCAGACTCAAAAAACCGAACAGATTTAACGTCTTTaaaaa                                                                                                      >  1:2943708/1‑146 (MQ=255)

                                                 aaaCGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCCACCGGTTGCGCAGACTCAAAAAAACGAAAAGATTTAACGTCTTGAAGAta                                                                                                     >  1:5204345/1‑148 (MQ=255)

                                                  aaCGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCCACCGGTTGCGCAGACTCAAAACAACGAACTGATTTAACGGCTTGAATATCg                                                                                                    >  1:639424/1‑149 (MQ=255)

                                                  aaCGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCCACCGGGTGCGCAGACTCAAAAAAGCGAACTGATTTAACGTCTTGAAGAtag                                                                                                    >  1:3149171/1‑147 (MQ=255)

                                                  aaCGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCACCCGGTTTCGCAGACTCAAAAAAGAGAACAGATTTTCCGTCATTAAGATCg                                                                                                    >  1:2850976/1‑149 (MQ=255)

                                                   aCGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGTATTGTTTTCGATGTCTTTTTCCACCCTCGAGTAAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGt                                                                                                   <  2:664431/149‑1 (MQ=255)

                                                   aCGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGt                                                                                                   <  2:1678462/149‑1 (MQ=255)

                                                       ttGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCCACCGGTTGCGCAGACTCAAAACATCGAACTGATTTAACGTCTTTAATATAGTccgc                                                                                               >  1:4242783/1‑149 (MQ=255)

                                                       ttGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTccgc                                                                                               >  2:21922/1‑149 (MQ=255)

                                                           ttttCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATAGTCcgccgcg                                                                                           >  2:893648/1‑148 (MQ=255)

                                                            tttCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCCGTTGCGCAGACACAAAACAACGAACAGATTTACCCTCTTGAAGAACCTCCCCCGCtt                                                                                          >  1:1203600/1‑149 (MQ=255)

                                                              tctcTTTCGGTCATCTAAAATGCCCGCTTTGCTACCAGGGTTTTTTTTTCGTTGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTcc                                                                                        <  1:2578168/149‑1 (MQ=255)

                                                              tctcTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGGTGCGCCGACTCAAAACAGCGAACAGATTTACCCTCTTGAAGATCGTCCGCCGCGTcc                                                                                        >  1:2803005/1‑149 (MQ=255)

                                                                      ggTCAGATAAAAGGCCCGCTTTGCTACCAGGGTTTTTTTTTCGATTTCTTTTTCCACCCTCGAGTCATAAATTGCGCAACCGGTGGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAAccc                                                                                <  1:5096349/149‑1 (MQ=255)

                                                                          agatAAAATGCCCGCTTTGCTACCACGGGTTTGTTTTCTTTGTCTGTTACCACCCTCGAGTCAGAAATTGCGCAACCGGTGGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGtt                                                                            <  1:4343858/146‑1 (MQ=255)

                                                                           gatAAAATGCCGTATTTGCTACCACGGTATTTTTTTCTATTTCTTTTTCAAACATCGAGTAAGAAAATGCGAAACCGTTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCTAACCCTGttt                                                                           <  2:2234672/147‑1 (MQ=255)

                                                                           gCTAAACGGCCCGCTTTGCTACCAGGGGTTTGTTTTCGTTGTCTGTTTCCCCCCTCGAGTCAGAAACTGCGCAACGGGGTGCCCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGttt                                                                           <  1:3275401/149‑1 (MQ=255)

                                                                            ctaattggCCCGTTTTGATACCTGGTGTTTTTTTTTGTTGTGTGTTTCCACCCTCTAGTAATAAAATGCGCAACGGGTGGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTg                                                                          <  1:2395202/142‑1 (MQ=255)

                                                                             tAAATTGCCCGTTTTGCTACCACGGTTTTTTTTTCTATGTCTTTTTCCACCCTCGTGTAATAAATTGCGCACCCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTgc                                                                         <  1:3863821/149‑1 (MQ=255)

                                                                             tAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTgc                                                                         >  2:1543057/1‑149 (MQ=255)

                                                                                aaTGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTgccgc                                                                      >  2:590977/1‑149 (MQ=255)

                                                                                  tGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAAACCTGTTTGCCGCCt                                                                    >  2:58737/1‑149 (MQ=255)

                                                                                      cGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTTAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCa                                                                >  2:473101/1‑149 (MQ=255)

                                                                                      cGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGGTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCGTCCTCAAACCCGGTTTGCCGCCTGTCa                                                                >  2:472575/1‑149 (MQ=255)

                                                                                        cTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGc                                                              >  2:3242543/1‑149 (MQ=255)

                                                                                           tGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAATTGCGCACCCGGTTGCGCAGACTCAAACCAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATc                                                           <  2:4508141/149‑1 (MQ=255)

                                                                                              tACCACGGTTTTGTTTTCGTTGTCTGTGTCCACCCTCGAGTCATAAACTGCGCACCGGGGGGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATctgc                                                        <  1:1474360/149‑1 (MQ=255)

                                                                                               aCCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCCTctgct                                                       >  2:1805330/1‑149 (MQ=255)

                                                                                               aCCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAAAAGCGAAAAGATTTAACGTCTTGAAAATCGTCCGCCGCGTCCACAAACCCAGTTTGCCGCCTGTCAGCATctgct                                                       >  1:2064706/1‑149 (MQ=255)

                                                                                                  aCGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCCGTTGCGCAGACACAAAAAAGCGAAATGATTTACCGGATTTAAGATAGTCCGCCGCCTCCACAAACCCAGTTTTCCGCCTGTCAGCAAATGCTGGc                                                    >  1:2981926/1‑149 (MQ=255)

                                                                                                      gATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATg                                                >  2:1936210/1‑149 (MQ=255)

                                                                                                         tGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAATTGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTc                                             <  2:3946491/149‑1 (MQ=255)

                                                                                                           ttttCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCCACCGGTTGCGCAGACTCAAAACAGAGAACTGATTTAACCTCTTTAATATAGTACGCCCCATCCACAAAACCTGTTTTCCGCCGGTCAGCAACTGCTGGCGCTGTTCGt                                           >  1:5130093/1‑149 (MQ=255)

                                                                                                              tCGTTGTCGGTTTCCACCCTCGAGACACAAATTGCCCACCCGGTGGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGc                                        <  1:2879373/149‑1 (MQ=255)

                                                                                                              tCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGc                                        <  2:2664180/149‑1 (MQ=255)

                                                                                                                tttGTCTTTTACCACAATCTAGACATAAATTGCTCAAACGTTTGCCCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAATATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGACTGTCAGCATCTGCTGGCGATGTTCGTTGCgg                                      <  1:4957781/147‑1 (MQ=255)

                                                                                                                 ttgtgtgTTCCCCCCCTCGAGCCAGAAAAGGCGCACCCGGTCGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGt                                     <  1:1070403/148‑1 (MQ=255)

                                                                                                                  tGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCCCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTg                                    <  1:3022160/149‑1 (MQ=255)

                                                                                                                   gTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCGTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGa                                   >  2:419968/1‑149 (MQ=255)

                                                                                                                    tCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGAt                                  <  2:3917662/149‑1 (MQ=255)

                                                                                                                    tCTGTTTCCACCCTAGATTCAGAAAATGCGCAAACGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGTAGATCGTCCGCCGCTTCCGCAAAACCTGTTTGGCGCCTGTACTCATCTGCTGGCGATGTTCGTTTCGGTGAt                                  >  2:56358/1‑149 (MQ=255)

                                                                                                                          tCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGcc                            >  2:4781648/1‑149 (MQ=255)

                                                                                                                            cACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGGCCGCCGCTTCCTCAAACCCGGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAg                          >  2:3608779/1‑149 (MQ=255)

                                                                                                                              cccTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCACAAACCCAGTTTTCCGCCTGTCAGCAACAGCAGGCGATCTTCGTTGCGGTGATCACCCCAgcg                        >  2:5130624/1‑149 (MQ=255)

                                                                                                                               ccTCTAGTAATAAACGGCGCACCGGGTGGCCCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAgcgc                       <  1:593916/149‑1 (MQ=255)

                                                                                                                               ccTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAgcgc                       >  2:3831386/1‑149 (MQ=255)

                                                                                                                                 tCGAGTCAGAAAAGGCGCACCGGGGTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCGGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTa                     <  2:4518274/149‑1 (MQ=255)

                                                                                                                                 gcgcgtaaGAAAAGGCGCACCGGGGGGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTa                     <  1:4042996/142‑1 (MQ=255)

                                                                                                                                      acAGAAAATGCGCAACCGGTGGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAAt                <  1:227331/148‑1 (MQ=255)

                                                                                                                                         gAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTg             <  2:2450197/149‑1 (MQ=255)

                                                                                                                                           aaaTGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTGcc           <  2:1658960/149‑1 (MQ=255)

                                                                                                                                           aaaTGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTGcc           <  2:2791497/149‑1 (MQ=255)

                                                                                                                                            aaTGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATAACGCCAGCGCTATTAATCTGCCa          >  1:2140843/1‑149 (MQ=255)

                                                                                                                                             tggcgcACCGGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTGCCAg         <  1:4166621/147‑1 (MQ=255)

                                                                                                                                             aTGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTGCCAg         >  1:3975873/1‑149 (MQ=255)

                                                                                                                                               gcccAACCGGTTGCCCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTGCCAGgc       <  1:5228211/146‑1 (MQ=255)

                                                                                                                                                 gcAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTATCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTGCCAGgcgc     <  2:2434058/149‑1 (MQ=255)

                                                                                                                                                 gcAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTGCCAGgcgc     <  1:1819189/149‑1 (MQ=255)

                                                                                                                                                 gcAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTGCCAGgcgc     <  2:3320972/149‑1 (MQ=255)

                                                                                                                                                    aCCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTGCCAGGCGCTgg  >  1:3655892/1‑149 (MQ=255)

TATAAAAAAGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTGCCAGGCGCTGG  >  CP009273/3822192‑3822488

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`