BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	3,822,345	T→G	7.4%	V14G (GTT→GGT)	xanP →	xanthine permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	3,822,345	0	T	G	7.4%	648.1 / 7.9	276	V14G (GTT→GGT)	xanP	xanthine permease
Reads supporting (aligned to +/- strand): ref base T (136/113); new base G (17/10); total (153/123)
Fisher's exact test for biased strand distribution p-value = 5.42e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 285 total aligned reads displayed.

AAAAGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTGCCAGGCGCTGGGTTTA  >  CP009273/3822197‑3822493

 aaaGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTCCGGTCAGCTAAAATGCCCACTGTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGtt                                                                                                                                                     <  2:3185167/149‑1 (MQ=255)

  aaGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTg                                                                                                                                                    <  2:1820246/149‑1 (MQ=255)

   aGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCCACCGGTTgc                                                                                                                                                   >  1:3320972/1‑149 (MQ=255)

        aTTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCaca                                                                                                                                              >  1:2883180/1‑147 (MQ=255)

        aTTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGCTTGCGCAGa                                                                                                                                              >  1:3550793/1‑149 (MQ=255)

         tttATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGAc                                                                                                                                             >  2:749876/1‑149 (MQ=255)

         tttATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGAc                                                                                                                                             >  2:3486614/1‑149 (MQ=255)

            aTCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCa                                                                                                                                          >  1:3373900/1‑149 (MQ=255)

            aTCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAAACCGTTTCGCAGACTCa                                                                                                                                          >  1:4101086/1‑149 (MQ=255)

               tcATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCCGTTGCGCAGACACaaaa                                                                                                                                       >  1:1711162/1‑149 (MQ=255)

               acATTTATATATTTAGATGAGTATGTGTAAAACTTTTGGTTTTATCTTTCGTTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATTTCTGTTTCCACCCGCGTGTCAGAAATTGCGCAACCGGTTGCGCAGACTCaaaa                                                                                                                                       <  2:5002103/148‑1 (MQ=255)

                  tttAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCCACCCGTTGAGCAGAAACaaaaaaa                                                                                                                                    >  1:3098935/1‑146 (MQ=255)

                      aaTATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGaa                                                                                                                                >  2:4619651/1‑149 (MQ=255)

                        tatTTAGATGAGGATGTGGCAACCGTTTGCTTTTCTCTTTCGGTCAGCGAAAATGCCGGCTTTGCTACCTCGGGATTGTTTTCTATGTCTGTTTCCCCCCTCGAGGCATAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACt                                                                                                                              <  2:636840/149‑1 (MQ=255)

                        tatTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCCGTTGCGCAGACTCAAAAAAGCGAAca                                                                                                                              >  1:219460/1‑148 (MQ=255)

                          tttAGATTAGGATGTGGCAAACGTTTGCTTAAAACATTCGGACAGCTAAAATGCCCGCTTTGCTACCACGGGATTGATTTCGAAGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAAACGCTTTCGCAGACACAAAACAGCGAAcata                                                                                                                            >  2:2596767/1‑146 (MQ=255)

                           ttAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCCGTTGCGCAGAATCAAAAAAACGAACAGaa                                                                                                                           >  1:968522/1‑148 (MQ=255)

                             aGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGAttt                                                                                                                         <  2:4766419/149‑1 (MQ=255)

                              gATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTa                                                                                                                        >  2:2327974/1‑149 (MQ=255)

                               aTGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCACACTCAAAACAGCGAACTGATTTAc                                                                                                                       >  1:848308/1‑149 (MQ=255)

                                tgtggttgtgGAAAACGTTTTCTTTTCTCTTTCGGGAAGATAAACGGCCCGCTGTGCTACCACGGTTTTTTTTTCGATGTCTGTTTCCACCCTCGATTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTAcc                                                                                                                      <  1:3442520/143‑1 (MQ=255)

                                tGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCCGGTGCGCAGACACAAAACAGCGAACAGAATTaac                                                                                                                      >  1:619869/1‑147 (MQ=255)

                                  aGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTGTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGt                                                                                                                    >  2:1557924/1‑149 (MQ=255)

                                   ggATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCCGACTCAAAACAGCGAACTGATTTAACGTc                                                                                                                   >  1:3720276/1‑149 (MQ=255)

                                    gATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGGTGCGCAGACACAAAAAAACGAACAGATTTAAAGTCt                                                                                                                  >  1:777563/1‑149 (MQ=255)

                                    gATGTGGAAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCTATGTCTGTTTCCACCCTCTAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCt                                                                                                                  <  2:4081037/149‑1 (MQ=255)

                                      tgtgGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCACCCCGTTGCGCCGACTCAAAACAGCGAACAGATTTACCGTCttt                                                                                                                >  1:1127864/1‑148 (MQ=255)

                                          gCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCACCCGGTTGCGCCGACTCAAAACAGCGAACTGATTTACCGTCTGGAAGa                                                                                                            >  2:1705802/1‑149 (MQ=255)

                                          gCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCCGTTGCGCAGACTCAAAAAAGCGCACGGATTTACCGTCGTGTAGa                                                                                                            >  1:1104562/1‑149 (MQ=255)

                                            aaaCGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATc                                                                                                          <  2:1517013/149‑1 (MQ=255)

                                            aaaCGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGATTTGCTACCACGGGATTGTTGTCGATGTCTGTTTCCACCCTCGAGTAATAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATc                                                                                                          <  2:2208708/149‑1 (MQ=255)

                                             aaCGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCCACCGGGTGCGCAGACTCAAAAAAGCGAACTGATTTAACGTCTTGAAGAtag                                                                                                         >  1:3149171/1‑147 (MQ=255)

                                             aaCGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCACCCGGTTTCGCAGACTCAAAAAAGAGAACAGATTTTCCGTCATTAAGATCg                                                                                                         >  1:2850976/1‑149 (MQ=255)

                                             aaCGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCg                                                                                                         >  2:5055833/1‑149 (MQ=255)

                                              aCGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCATAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGt                                                                                                        <  2:664294/149‑1 (MQ=255)

                                              aCGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGt                                                                                                        <  2:1701301/149‑1 (MQ=255)

                                               cGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGTTTGTTTTCGATGTCTGTTTCTACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTc                                                                                                       <  2:237251/149‑1 (MQ=255)

                                                 tttGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAAAAGCGAACAGATTTACCGTCTTTAAGATCGTccg                                                                                                     >  1:3701064/1‑149 (MQ=255)

                                                  ttGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCCACCGGTTGCGCAGACTCAAAACATCGAACTGATTTAACGTCTTTAATATAGTccgc                                                                                                    >  1:4242783/1‑149 (MQ=255)

                                                      ttttCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATAGTCcgccgcg                                                                                                >  2:893648/1‑148 (MQ=255)

                                                      ttttCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGAAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCt                                                                                                >  2:2863221/1‑149 (MQ=255)

                                                      ttttATCTTTCGGACATCTAAACTGCCCTATTTGCTACCTCGGGTTTGTTTTCGATGTCTTTTTCCACCCTCTAGTAATAAATTGCGCAACGGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCt                                                                                                <  1:2440094/149‑1 (MQ=255)

                                                        ttCTCTTTCGGTCAGCTAAAAGGCCCGCTTTGATACCCGGGTTTTGTTTTCGATGTCTGTTTCCACCCTCGAGTCATAAAATGCGCACCGGGTGGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTc                                                                                              <  1:1465768/149‑1 (MQ=255)

                                                         tctcTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCCACCGGTTGCGCAGACTCAAAACAGCGAACAGATTTACCGGCTTGAATATAGTCCGCCGCTGcc                                                                                             >  1:2803544/1‑149 (MQ=255)

                                                         tctcTTTCGGCCAGCAAAAAGGCCCGCTTTGCAACCACGGGTTTGTTTTCGATGTCTGTTACCACCCTCGATTCCGAAAATGCCCACCGGGTTGCCCAGACTCAAACCAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTcc                                                                                             <  1:2578354/149‑1 (MQ=255)

                                                                 gttctgCTAAAATGCCCGCTTTGCTTCCTGGTTATTTTTTTCTATGTCTGTTTCCACCATCGTGTCTGAATATGCGAAACCGGTTGCGCAGAATCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAAccc                                                                                     <  2:574221/144‑1 (MQ=255)

                                                                 ggTCAGATAAAAGGCCCGCTTTGCTACCAGGGTTTTTTTTTCGATTTCTTTTTCCACCCTCGAGTCATAAATTGCGCAACCGGTGGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAAccc                                                                                     <  1:5096349/149‑1 (MQ=255)

                                                                     agatAAAATGCCCGCTTTGCTACCACGGGTTTGTTTTCTTTGTCTGTTACCACCCTCGAGTCAGAAATTGCGCAACCGGTGGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGtt                                                                                 <  1:4343858/146‑1 (MQ=255)

                                                                      tcTAAATTGCCCGCTTTGCTACCTCGTTTTTGTTTTCGATGTCTGTTTACACCCTCGTTTCATAAATTGCGAACGGGGTTGCCCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGttt                                                                                <  1:3274797/148‑1 (MQ=255)

                                                                           aaTGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTgccgc                                                                           >  2:590944/1‑149 (MQ=255)

                                                                            aTGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTgccgcc                                                                          <  2:4912838/149‑1 (MQ=255)

                                                                                 cGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTTAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCa                                                                     >  2:473101/1‑149 (MQ=255)

                                                                                  gCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAg                                                                    <  2:4710750/149‑1 (MQ=255)

                                                                                   cTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGCGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCGTCCTCAAACCCAGTTTGCCGCCTGTCCGc                                                                   >  2:4214153/1‑149 (MQ=255)

                                                                                   cTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGc                                                                   >  2:1304756/1‑149 (MQ=255)

                                                                                   cTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACAGATTTACCCTCTTTAAGATCGTCCGCCGCTTCCTCAAACCCAGTTTGCCGCCGGTCAGc                                                                   >  1:2085484/1‑149 (MQ=255)

                                                                                    tttGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCGTCCTCAAACCCTGTTTGCCGCCAGTCAGCa                                                                  >  2:4847756/1‑149 (MQ=255)

                                                                                      tGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAATTGCGCACCCGGTTGCGCAGACTCAAACCAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATc                                                                <  2:4508141/149‑1 (MQ=255)

                                                                                       gCTACCACGGGAGTGTTTTCGATGTCTGTGTCCACCCTCGAGTCAGAAAATGCGCAACCGGTGGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAACATct                                                               <  2:2766562/149‑1 (MQ=255)

                                                                                          aCCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCCTctgct                                                            >  2:1805330/1‑149 (MQ=255)

                                                                                          aCCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAAAAGCGAAAAGATTTAACGTCTTGAAAATCGTCCGCCGCGTCCACAAACCCAGTTTGCCGCCTGTCAGCATctgct                                                            >  1:2064706/1‑149 (MQ=255)

                                                                                               gttaTTGTTTTCGTTGTCTGTTACCACCCTCTAGTAACAAAATGCGCACCCGGTGGCCCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGa                                                       <  1:2951248/146‑1 (MQ=255)

                                                                                               gggAGTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGa                                                       >  2:3797072/1‑149 (MQ=255)

                                                                                                  tttGTTTTCTATGTGTGTTACCACCCGCGAGAAACAAACTGCGCACCGGGGCGCCCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGt                                                    <  1:459646/148‑1 (MQ=255)

                                                                                                      ttttCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCCACCGGTTGCGCAGACTCAAAACAGAGAACTGATTTAACCTCTTTAATATAGTACGCCCCATCCACAAAACCTGTTTTCCGCCGGTCAGCAACTGCTGGCGCTGTTCGt                                                >  1:5130093/1‑149 (MQ=255)

                                                                                                      ttttCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGt                                                <  2:294115/149‑1 (MQ=255)

                                                                                                         tCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGc                                             <  2:2664180/149‑1 (MQ=255)

                                                                                                           tttGTCTTTTACCACAATCTAGACATAAATTGCTCAAACGTTTGCCCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAATATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGACTGTCAGCATCTGCTGGCGATGTTCGTTGCgg                                           <  1:4957781/147‑1 (MQ=255)

                                                                                                           gATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCGTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCgg                                           <  2:3923490/149‑1 (MQ=255)

                                                                                                            ttgtgtgTTCCCCCCCTCGAGCCAGAAAAGGCGCACCCGGTCGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGt                                          <  1:1070403/148‑1 (MQ=255)

                                                                                                             tGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCCCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTg                                         <  1:3022160/149‑1 (MQ=255)

                                                                                                              gTCTTTTTCCCCCCTCGCGTCAGAAACTGCGCAACGGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGa                                        <  1:3930259/149‑1 (MQ=255)

                                                                                                               tCTGTTTCCACCCTAGATTCAGAAAATGCGCAAACGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGTAGATCGTCCGCCGCTTCCGCAAAACCTGTTTGGCGCCTGTACTCATCTGCTGGCGATGTTCGTTTCGGTGAt                                       >  2:56358/1‑149 (MQ=255)

                                                                                                                cTGTTTCCACCCTCGTGGCAGAAAATGCGCACCCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATc                                      <  2:360469/149‑1 (MQ=255)

                                                                                                                   tttCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTACTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACg                                   >  2:2310555/1‑149 (MQ=255)

                                                                                                                       cACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGCAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGcccg                               >  2:3608805/1‑147 (MQ=255)

                                                                                                                       cACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGCTGTTCGTTGCGGTGATCACGCCAg                               >  2:2341940/1‑149 (MQ=255)

                                                                                                                        aCCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAgc                              <  2:1088597/149‑1 (MQ=255)

                                                                                                                        aCCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAgc                              <  2:3511021/149‑1 (MQ=255)

                                                                                                                          ccTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAgcgc                            >  2:3831581/1‑149 (MQ=255)

                                                                                                                          ccTCGAGACCGAAATGGCGCACCCGGGGGCGCAGACTCAAAACAGCGAACTGATGGACCGTCTTGAAGATCGGCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAgcgc                            <  2:5142767/149‑1 (MQ=255)

                                                                                                                           cTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCt                           >  2:2048928/1‑149 (MQ=255)

                                                                                                                            tCGAGTCAGAAAAGGCGCACCGGGGTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCGGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTa                          <  2:4518274/149‑1 (MQ=255)

                                                                                                                            gcgcgtaaGAAAAGGCGCACCGGGGGGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTa                          <  1:4042996/142‑1 (MQ=255)

                                                                                                                                 acAGAAAATGCGCAACCGGTGGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAAt                     <  1:227331/148‑1 (MQ=255)

                                                                                                                                    gAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTg                  <  2:2450197/149‑1 (MQ=255)

                                                                                                                                     aaaaTGCGCACCCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTGc                 <  2:1164794/149‑1 (MQ=255)

                                                                                                                                     aaaaTGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTGc                 <  2:1589456/149‑1 (MQ=255)

                                                                                                                                      aattGCGCAACCGGTGGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTGcc                <  2:166032/146‑1 (MQ=255)

                                                                                                                                       aaTGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATAACGCCAGCGCTATTAATCTGCCa               >  1:2140843/1‑149 (MQ=255)

                                                                                                                                        tggcgcACCGGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTGCCAg              <  1:4166621/147‑1 (MQ=255)

                                                                                                                                        aTGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTGCCAg              >  1:3975873/1‑149 (MQ=255)

                                                                                                                                          gcgcAACGGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTGCCAGgc            <  1:5228501/149‑1 (MQ=255)

                                                                                                                                          gcccAACCGGTTGCCCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTGCCAGgc            <  1:5228211/146‑1 (MQ=255)

                                                                                                                                            gcAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTGCCAGgcgc          <  2:3320972/149‑1 (MQ=255)

                                                                                                                                            gcAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTGCCAGgcgc          <  1:1819189/149‑1 (MQ=255)

                                                                                                                                             cAACGGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTGCCAGGCGCt         <  1:5178701/149‑1 (MQ=255)

                                                                                                                                                 cGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTGCCAGGCGCTGGGt     <  2:1063154/149‑1 (MQ=255)

                                                                                                                                                    ttGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTGCCAGGCGCTGGGTTTa  <  2:4242783/149‑1 (MQ=255)

                                                                                                                                                    ttGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTGCCAGGCGCTGGGTTTa  >  1:3245760/1‑149 (MQ=255)

AAAAGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTGCCAGGCGCTGGGTTTA  >  CP009273/3822197‑3822493

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`