BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	3,860,159	C→A	87.1%	P33P (CCG→CCT)	ibpB ←	heat shock chaperone

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	3,860,159	0	C	A	87.1%	652.3 / 87.5	267	P33P (CCG→CCT)	ibpB	heat shock chaperone
Reads supporting (aligned to +/- strand): ref base C (17/18); new base A (86/143); total (106/161)
Fisher's exact test for biased strand distribution p-value = 2.64e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.68e-01

Only 100 of 269 total aligned reads displayed.

TTTTTCTCTTCTTTTGGCTGCTCCGGCGTGCCTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGCGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTT‑GGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAA  >  CP009273/3860013‑3860303

tttttCTCTTCTTTTGGCTGCTCCGGCGTGCCTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTAcggagg                                                                                                                                                 <  2:799272/149‑1 (MQ=255)

 ttttCTCTTCTTTTGGCTGCTCCGGCGTGCCTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAggg                                                                                                                                                <  2:2357458/149‑1 (MQ=255)

 ttttCTCTTCTTTTGGCTGCTCCGGCGTGCCTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAggg                                                                                                                                                <  1:4374491/149‑1 (MQ=255)

  tttCTCTTCTTTTGGCTGCTCCGGCGTGCCTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGa                                                                                                                                               <  2:279217/149‑1 (MQ=255)

  tttCTCTTCTTTTGGCTGCTCCGGCGTGCCTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGa                                                                                                                                               <  2:1184292/149‑1 (MQ=255)

  tttCTCTTCTTTTGGCTGCTCCGGCGTGCCTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTATGAATGGTGTTCGGAgggg                                                                                                                                               >  1:4186011/1‑148 (MQ=255)

   ttCTCTTCTTTTGGCTGCTCCGGCGTGCCTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTATCAAATTTGTAAGGAGGGaa                                                                                                                                              >  1:2738459/1‑149 (MQ=255)

    tctcTTCTTTTGGCTGCTCCGGCGTGCCTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAg                                                                                                                                             <  1:3092388/149‑1 (MQ=255)

           ttttGGCTGCTCCGGCGTGCCTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGc                                                                                                                                      >  1:1730328/1‑149 (MQ=255)

           ttttGGCTGCTCCGGCGTGCCTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGc                                                                                                                                      >  2:2918370/1‑149 (MQ=255)

               ggCTGCTCCGGCGTGCCTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTc                                                                                                                                  <  2:2046653/149‑1 (MQ=255)

                    ctCCGGCGTGCCTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCgg                                                                                                                             <  1:1413062/149‑1 (MQ=255)

                      ccGGCGTGCCTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCg                                                                                                                           <  1:2810382/149‑1 (MQ=255)

                       cGGCGTGCCTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGt                                                                                                                          <  1:3384882/149‑1 (MQ=255)

                         gCGTGCCTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGttt                                                                                                                        <  2:45385/149‑1 (MQ=255)

                          cGTGCCTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGtttt                                                                                                                       <  2:1944778/149‑1 (MQ=255)

                           gTGCCTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTg                                                                                                                      >  2:385516/1‑149 (MQ=255)

                           gTGCCTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCGCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTg                                                                                                                      >  2:3031007/1‑149 (MQ=255)

                            tGCCTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGc                                                                                                                     <  1:924710/149‑1 (MQ=255)

                               cTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGt                                                                                                                  <  2:177906/149‑1 (MQ=255)

                               cTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGt                                                                                                                  <  2:3693079/149‑1 (MQ=255)

                                ttttACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTATCAATGTTGTACGGAGGGCAGCTCTGGCTTTCAACGGCGTTTTGCAGTg                                                                                                                 >  1:3444672/1‑149 (MQ=255)

                                ttttACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTATAAATTTTGTACGGAGGGAAGCTCTGGCTTTAACAGGCGTTTTTCAGtt                                                                                                                 >  2:4183265/1‑148 (MQ=255)

                                 tttACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGATGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGc                                                                                                                >  2:341043/1‑149 (MQ=255)

                                   tACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGt                                                                                                              <  1:2888281/149‑1 (MQ=255)

                                   tACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGt                                                                                                              <  2:4693701/149‑1 (MQ=255)

                                      gCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTT‑gg                                                                                                          >  2:2139660/1‑149 (MQ=255)

                                       cTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTT‑GGc                                                                                                         <  1:513586/149‑1 (MQ=255)

                                       cTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTT‑GGc                                                                                                         <  2:3353291/149‑1 (MQ=255)

                                       cTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTT‑GGc                                                                                                         <  1:2265980/149‑1 (MQ=255)

                                          aGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTT‑GGCCAg                                                                                                      <  1:2187767/149‑1 (MQ=255)

                                           ggCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAATCTCTGGCGTTCACCGGCGTTTTGCAGTGCGTT‑GGCCAGt                                                                                                     >  2:336272/1‑149 (MQ=255)

                                           ggCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTT‑GGCCAGt                                                                                                     <  2:4391492/149‑1 (MQ=255)

                                           ggCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTT‑GGCCAGt                                                                                                     <  1:2535837/149‑1 (MQ=255)

                                             cgcgTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTT‑GGCCAGttt                                                                                                   <  1:1731438/149‑1 (MQ=255)

                                              gcgTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCGTTTCTCAATGGTTTAAGGCGGGAAGCTCTGGCTTTAACCGGCGTTTTGCAGTGCGGT‑GGCCAGGTTg                                                                                                  >  2:2235258/1‑149 (MQ=255)

                                               cgTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTT‑GGCCAGTTTGt                                                                                                 <  1:3572096/149‑1 (MQ=255)

                                                 tACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTATAAATTTTTTACGGAGGGAAGCTCTGGCTTTAACCGGCGTTTTGCAGTGCGTT‑GGGCAGTTTGTCa                                                                                               >  2:2294397/1‑149 (MQ=255)

                                                  aCCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTAAATTTTTTACGGTGGGTAGCTCAGGCGTTCAACGGCGTTTTTCAGTTCGTT‑GGCCAGTTTTTCaa                                                                                              >  1:3896634/1‑149 (MQ=255)

                                                   ccTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTATAAATGTTTTACGGCGGGAAGCTCTGGCTTTCACCGGCGTTTTTCAGTGCGTT‑GGGCCGTTTGTGaaa                                                                                             >  1:100738/1‑149 (MQ=255)

                                                     ttCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGCAGCTCTGGCGTTCACCGGCGTTTTGCAGTGCGTTGGGCCCTTTTGCCaaaa                                                                                            >  2:647016/1‑149 (MQ=255)

                                                        cAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTT‑GGCCAGTTTGTCAAAACCGa                                                                                        <  1:1162911/149‑1 (MQ=255)

                                                           ttGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTT‑GGCCAGTTTGTCAAAACCGATcc                                                                                     <  1:4325647/149‑1 (MQ=255)

                                                            tGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTT‑GGCCAGTTTGTCAAAACCGATCCa                                                                                    <  1:2413552/149‑1 (MQ=255)

                                                               aTCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTT‑GGCCAGTTTGTCAAAACCGATCCATTg                                                                                 <  2:4939127/149‑1 (MQ=255)

                                                                tctcTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTGTCAATGTTGTTCGGAGGGAAGCTCTGGCTTTCACCGGCGGTTTGCAGTTCGGT‑GGGCAGGTTTTAAAAACCCATACAATGa                                                                                >  1:3786144/1‑149 (MQ=255)

                                                                tctcTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGCGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTT‑GGCCAGTTTGTCAAAACCGATCCATTGa                                                                                <  1:5013723/149‑1 (MQ=255)

                                                                tctcTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTT‑GGCCAGTTTGTCAAAACCGATCCATTGa                                                                                <  1:2696594/149‑1 (MQ=255)

                                                                  tctAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTGTCCATGTTGTTAGGCGGGGAGCTCTGGCGTTGACCGGGGGTTTTGAGTGCGGT‑GGGCCGGTTGTGAAAAACGATcccattacg                                                                              >  1:4546143/1‑142 (MQ=255)

                                                                      aaTCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTT‑GGCCAGTTTGTCAAAACCGATCCATTGACGCATc                                                                          <  1:4820540/149‑1 (MQ=255)

                                                                      aaTCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTT‑GGCCAGTTTGTCAAAACCGATCCATTGACGCATc                                                                          <  1:3860078/149‑1 (MQ=255)

                                                                         cTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGCGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTT‑GGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGt                                                                       <  1:302849/149‑1 (MQ=255)

                                                                         cTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTT‑GGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGt                                                                       <  2:2925138/149‑1 (MQ=255)

                                                                         cTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTT‑GGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGt                                                                       <  2:755714/149‑1 (MQ=255)

                                                                          ttCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTT‑GGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTg                                                                      <  2:465980/149‑1 (MQ=255)

                                                                           tCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGCGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTT‑GGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTgg                                                                     <  2:4127581/149‑1 (MQ=255)

                                                                            ccTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTT‑GGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTggg                                                                    <  2:568301/149‑1 (MQ=255)

                                                                                 cGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTATCAAAGTTGTAAGGAGGGAAGCGCTGGCTTTAACCCGCGTTTTGGAGTGCGTT‑GGCCCCTTTTTGAAAACCGATCCCATTTCGCCTCAGGGGGGAtta                                                               >  1:3201707/1‑147 (MQ=255)

                                                                                   gAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAAAGTTGTACGGAGGGACGCTCTGGCTTTAACCGGCGTTTTGCAGTGCGTT‑GGCCAGTTTGTCAAAACCCATACATTGACGCATCAGTGGGGGTAAAt                                                             >  2:2565482/1‑149 (MQ=255)

                                                                                   gAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTATCAATGTTGTACGGAGGGAAGCTCTGGCGTTCACCGGCGTTTTTCAGTGCGTT‑GGGCCGTTTTTAAAAAACGATACAATGACGCATCAGTGGGGCTAAAt                                                             >  2:1447607/1‑149 (MQ=255)

                                                                                   gAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTATCAATGTTGTAACGAGGGAAGCGCTGGCTTTAAACGGCGTTTTTCAGTGCGGT‑GGCCAGTTTTTCAAAAACCATACAATGACGCAAAAGTGGGGATaaaa                                                             >  1:3771206/1‑148 (MQ=255)

                                                                                    aaaCCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTT‑GGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATc                                                            <  1:1678503/149‑1 (MQ=255)

                                                                                      aCCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTT‑GGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGa                                                          <  1:3509409/149‑1 (MQ=255)

                                                                                       ccTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTT‑GGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGaa                                                         <  1:1098156/149‑1 (MQ=255)

                                                                                       ccTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTT‑GGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGaa                                                         <  2:595924/149‑1 (MQ=255)

                                                                                         tGCCAGCGCAAGGGTAATGCGGTTGTGGTTATCGTCGCTGTTCTCTTTTTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGAGTT‑GGCCTGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGATGt                                                       <  1:1304476/149‑1 (MQ=255)

                                                                                         tGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTT‑GGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGt                                                       <  2:2349593/149‑1 (MQ=255)

                                                                                         tGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTT‑GGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGt                                                       <  1:5241000/149‑1 (MQ=255)

                                                                                          gCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCAACGGCGTTTTGCAGTGCGGT‑TGCCAGTTTGTAAAAACCGATCCATTGACGCAGCAGTGGGGATAAATAGAAGtt                                                      >  2:686950/1‑149 (MQ=255)

                                                                                              gcgcAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTT‑GGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGc                                                  <  2:3291917/149‑1 (MQ=255)

                                                                                                   aGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTT‑GGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGt                                             <  1:2223678/149‑1 (MQ=255)

                                                                                                    gggTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTT‑GGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTc                                            <  2:1590533/149‑1 (MQ=255)

                                                                                                    gggTAATGCGGTAGTGGTTATCGTCGCTTTTATCAATTTTGTACGGAGGGAAGCTCTGGCGTTCACCGGCGTTTTGCAGTGCGTT‑TGCCCGGTTTTCAAAACACAACCAATGACGCATCAGTGGGGATAAATAGAAGTTAAGCAAAGta                                            >  2:903709/1‑148 (MQ=255)

                                                                                                     ggTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTT‑GGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCa                                           <  1:4170041/149‑1 (MQ=255)

                                                                                                      gTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTT‑GGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCAt                                          <  1:650002/149‑1 (MQ=255)

                                                                                                       tAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTT‑GGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCAtt                                         <  2:2666888/149‑1 (MQ=255)

                                                                                                       tAATGCGGTAGTGGTTATCGTCGCTTTTATCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGTAGTGCGTT‑GGCCAGTTTGTCAAAACCGATACAATTACGCATCAGTGGGGATAAATAGAAGTTACGCATAGTCAtt                                         >  2:315199/1‑149 (MQ=255)

                                                                                                        aaTGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCGTTCACCCGCGTTTTGCAGTGCGGT‑TGCCAGTTTGTCAAAAACCATCCAATGACGCCTCAGTGGGGATAAAAACAAGTTAACCATATTCAttt                                        >  1:821092/1‑149 (MQ=255)

                                                                                                         aTGCGGTAGTGGTTATCGTCGCTTTTATCAATTTTGTAAGGAGGGAATCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTT‑GGCCCCTTTGTCAAAAACCATACAATGACGCATAAGTGGGGATAAATCGAAGTTACGCATATTAAttta                                       >  2:1044751/1‑148 (MQ=255)

                                                                                                          tGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGCGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTT‑GGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTtct                                      <  2:2758431/149‑1 (MQ=255)

                                                                                                                 gTGGTTATCGTCGCTTTTCTCAATGTTTTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTTCAGTGCGTT‑GGCCAGTTTGTCAAAACCGATCCATTTACGCATCAGTGGGGATAAATAGAAGTTACGCATAGTAATTTCTACATCTa                               >  2:2944352/1‑149 (MQ=255)

                                                                                                                    gTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCGTTCACCGGCGTTTTGCACTGCGTT‑GGCCAGTTTGTCAAAACCCATCCATTGACGCAACAGTGGGGATAAATAGAAGTTACGCAAAGTCATTTCTCCATATaaga                            >  2:3923026/1‑149 (MQ=255)

                                                                                                                        tcgtcgCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTT‑GGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCg                        <  2:5219355/149‑1 (MQ=255)

                                                                                                                          gtcgCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTT‑GGCCAGTTTGTCAAAACCCATACATTGAAGCATCAGTGGGGATAAATAGAAGTTAAGCAAAGTCAATTATCCGTCAAAGAATCgtg                      >  1:1762522/1‑147 (MQ=255)

                                                                                                                           tcgCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTT‑GGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGagt                     <  1:4285466/149‑1 (MQ=255)

                                                                                                                            cgCTTTTCTCATTGTTGTACGGCGGGAAGCTCTGGCTTTCTCCGGCGTTTTGCAGTGCGTT‑GGCCAGTTTGTCAAAACAGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGagta                    <  1:1329711/149‑1 (MQ=255)

                                                                                                                            cgCTTTTCTCAATGTTGTACGGCGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTT‑GGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGagta                    <  1:4056190/149‑1 (MQ=255)

                                                                                                                            cgCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTT‑GGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGagta                    >  1:1682150/1‑149 (MQ=255)

                                                                                                                              cTTTTCTCAATGTTGTACGGCGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTT‑GGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGagtaag                  >  2:508288/1‑149 (MQ=255)

                                                                                                                              cTTTTCTCAATGTTGTACGGCGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTT‑GGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGagtaag                  >  2:508594/1‑149 (MQ=255)

                                                                                                                                  tctcAATGTTGTACGGCGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTT‑GGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTAcc              <  2:4357596/149‑1 (MQ=255)

                                                                                                                                  tctcAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTT‑GGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTAcc              <  2:278343/149‑1 (MQ=255)

                                                                                                                                    tcAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTT‑GGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTg            <  2:3233260/149‑1 (MQ=255)

                                                                                                                                     cAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTT‑GGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGc           >  2:3774372/1‑149 (MQ=255)

                                                                                                                                      aaTGGTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTT‑GGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCa          <  2:4569903/149‑1 (MQ=255)

                                                                                                                                        tgttgtACGGCGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTT‑GGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCaaa        <  2:1530456/149‑1 (MQ=255)

                                                                                                                                          ttgtACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTT‑GGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATc      >  1:4714467/1‑149 (MQ=255)

                                                                                                                                          ttgtACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTT‑GGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATc      <  2:1653922/149‑1 (MQ=255)

                                                                                                                                          ttgtACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTT‑GGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATc      <  2:2521414/149‑1 (MQ=255)

                                                                                                                                             tACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTT‑GGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGa   >  2:4213337/1‑149 (MQ=255)

TTTTTCTCTTCTTTTGGCTGCTCCGGCGTGCCTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGCGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTT‑GGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAA  >  CP009273/3860013‑3860303

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`