BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	3,869,280	G→T	10.9%	M161I (ATG→ATT)	yidX →	putative lipoprotein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	3,869,280	0	G	T	10.9%	1076.7 / 82.7	302	M161I (ATG→ATT)	yidX	putative lipoprotein
Reads supporting (aligned to +/- strand): ref base G (136/133); new base T (19/14); total (155/147)
Fisher's exact test for biased strand distribution p-value = 4.67e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.46e-01

Only 100 of 310 total aligned reads displayed.

TTACCGGCACAATATCGCTTTACTACTACTGGAATAACGAAGAAGAAAAGGCACTGGCAACGCATTATGGTTTTGCCTGTGGTGTTCAACACTGTACCAGGTCACTGGAAAACCTAAAAGGCACAATCCATGAGAAAAATAAAAACATGGATTACTCAAAGGTGATGGCGTTCTACCATCCATTTAAAGTGCGATTTTATGAATACTATTCACCCAGAGGCATTCCGGATGGTGTTTCCGCAGCATTACTGCCAGTGACTGTTACGCTGGACATCATTACTGCACCGCTGCAATTT  >  CP009273/3869132‑3869427

ttACCGGCACAATATCGCTTTACTACTACTGGAATAACGAAGAAGAAAAGGCACTGGCAACGCATTATGGTTTTGCCTGTGGTGTTCAACACTGTACCAGGTCACTGGAAAACCTAAAAGGCACAATCCATGAGAAAAATAAAAACATg                                                                                                                                                     <  2:4347685/149‑1 (MQ=255)

 tACCGGCACAATATCGCTTTACTACTACTGGAATAACGAAGAAGAAAAGGCACTGGCAACGCATTATGGTTTTGCCTGTGGTGTTCAACACTGTACCAGGTCACTGGAAAACCTAAAAGGCACAATCCATGAGAAAAATAAAAACATgg                                                                                                                                                    >  1:2827289/1‑149 (MQ=255)

 tACCGGCACAATATCGCTTTACTACTACTGGAATAACGAAGAAGAAAAGGCACTGGCAACGCATTATGGTTTTGCCTGTGGTGTTCAACACTGTACCAGGTCACTGGAAAACCTAAAAGGCACAATCCATGAGAAAAATAAAAACATgg                                                                                                                                                    >  2:3643650/1‑149 (MQ=255)

    cGGCACAATATCGCTTTACTACTACTGGAATAACGAAGAAGAAAAGGCACTGGCAACGCATTATGGTTTTGCCTGTGGTGTTCAACACTGTACCAGGTCACTGGAAAACCTAAAAGGCACAATCCATGAGAAAAATAAAAACATGGAtt                                                                                                                                                 <  1:4502963/149‑1 (MQ=255)

    cGGCACAATATCGCTTTACTACTACTGGAATAACGAAGAAGAAAAGGCACTGGCAACGCATTATGGTTTTGCCTGTGGTGTTCAACACTGTACCAGGTCACTGGAAAACCTAAAAGGCACAATCCATGAGAAAAATAAAAACATGGAtt                                                                                                                                                 <  1:1816770/149‑1 (MQ=255)

             atCGCTTTACTACTACTGGAATAACGAAGAAGAAAAGGCACTGGCAACGCATTATGGTTTTGCCTGTGGTGTTCAACACTGTACCAGGTCACTGGAAAACCTAAAAGGCACAATCCATGAGAAAAATAAAAACATGGATTACTCAAAgg                                                                                                                                        <  2:1144403/149‑1 (MQ=255)

             atCGCTTTACTACTACTGGAATAACGAAGAAGAAAAGGCACTGGCAACGCATTATGGTTTTGCCTGTGGTGTTCAACACTGTACCAGGTCACTGGAAAACCTAAAAGGCACAATCCATGAGAAAAATAAAAACATGGATTACTCAAAgg                                                                                                                                        >  2:1408389/1‑149 (MQ=255)

              tCGCTTTACTACTACTGGAATAACGAAGAAGAAAAGGCACTGGCAACGCATTATGGTTTTGCCTGTGGTGTTCAACACTGTACCAGGTCACTGGAAAACCTAAAAGGCACAATCCATGAGAAAAATAAAAACATGGATTACTCAAAGGt                                                                                                                                       >  1:1045433/1‑149 (MQ=255)

                   ttACTACTACTGGAATAACGAAGAAGAAAAGGCACTGGCAACGCATTATGGTTTTGCCTGTGGTGTTCAACACTGTACCAGGTCACTGGAAAACCTAAAAGGCACAATCCATGAGAAAAATAAAAACATGGATTACTCAAAGGTGATgg                                                                                                                                  <  1:142782/149‑1 (MQ=255)

                    tactactacTGGAATAACGAAGAAGAAAAGGCACTGGCAACGCATTATGGTTTTGCCTGTGGTGTTCAACACTGTACCAGGTCACTGGAAAACCTAAAAGGCACAATCCATGAGAAAAATAAAAACATGGATTACTCAAAGGTGATGGc                                                                                                                                 >  2:4174273/1‑149 (MQ=255)

                     actactactGGAATAACGAAGAAGAAAAGGCACTGGCAACGCATTATGGTTTTGCCTGTGGTGTTCAACACTGTACCAGGTCACTGGAAAACCTAAAAGGCACAATCCATGAGAAAAATAAAAACATGGATTACTCAAAGGTGATGGCg                                                                                                                                >  2:3944909/1‑149 (MQ=255)

                       tactactGGAATAACGAAGAAGAAAAGGCACTGGCAACGCATTATGGTTTTGCCTGTGGTGTTCAACACTGTACCAGGTCACTGGAAAACCTAAAAGGCACAATCCATGAGAAAAATAAAAACATGGATTACTCAAAGGTGATGGCGtt                                                                                                                              <  1:4144767/149‑1 (MQ=255)

                         ctactGGAATAACGAAGAAGAAAAGGCACTGGCAACGCATTATGGTTTTGCCTGTGGTGTTCAACACTGTACCAGGTCACTGGAAAACCTAAAAGGCACAATCCATGAGAAAAATAAAAACATGGATTACTCAAAGGTGATGGCGTTCt                                                                                                                            >  1:5004369/1‑149 (MQ=255)

                         ctactGGAATAACGAAGAAGAAAAGGCACTGGCAACGCATTATGGTTTTGCCTGTGGTGTTCAACACTGTACCAGGTCACTGGAAAACCTAAAAGGCACAATCCATGAGAAAAATAAAAACATGGATTACTCAAAGGTGATGGCGTTCt                                                                                                                            <  1:2476530/149‑1 (MQ=255)

                             tGGAATAACGAAGAAGAAAAGGCACTGGCAACGCATTATGGTTTTGCCTGTGGTGTTCAACACTGTACCAGGTCACTGGAAAACCTAAAAGGCACAATCCATGAGAAAAATAAAAACATGGATTACTCAAAGGTGATGGCGTTCTAcca                                                                                                                        >  1:3597667/1‑149 (MQ=255)

                              ggAATAACGAAGAAGAAAAGGCACTGGCAACGCATTATGGTTTTGCCTGTGGTGTTCAACACTGTACCAGGTCACTGGAAAACCTAAAAGGCACAATCCATGAGAAAAATAAAAACATGGATTACTCAAAGGTGATGGCGTTCTAccat                                                                                                                       >  1:3376593/1‑149 (MQ=255)

                              ggAATAACGAAGAAGAAAAGGCACTGGCAACGCATTATGGTTTTGCCTGTGGTGTTCAACACTGTACCAGGTCACTGGAAAACCTAAAAGGCACAATCCATGAGAAAAATAAAAACATGGATTACTCAAAGGTGATGGCGTTCTAccat                                                                                                                       <  1:255771/149‑1 (MQ=255)

                                aaTAACGAAGAAGAAAAGGCACTGGCAACGCATTATGGTTTTGCCTGTGGTGTTCAACACTGTACCAGGTCACTGGAAAACCTAAAAGGCACAATCCATGAGAAAAATACAAACATGGAGTACTCAAAGGTGATGGCGTTCTAcaatcc                                                                                                                     >  1:940855/1‑149 (MQ=255)

                                aaTAACGAAGAAGAAAAGGCACTGGCAACGCATTATGGTTTTGCCTGTGGTGTTCAACACTGTACCAGGTCACTGGAAAACCTAAAAGGCACAATCCATGAGAAAAATAAAAACATGGATTACTCAAAGGTGATGGCGTTCTAccatcc                                                                                                                     >  1:3523110/1‑149 (MQ=255)

                                aaTAACGAAGAAGAAAAGGCACTGGCAACGCATTATGGTTTTGCCTGTGGTGTTCAACACTGTACCAGGTCACTGGAAAACCTAAAAGGCACAATCCATGAGAAAAATAAAAACATGGATTACTCAAAGGTGATGGCGTTCTAccatcc                                                                                                                     >  2:2836912/1‑149 (MQ=255)

                                 aTAACGAAGAAGAAAAGGCACTGGCAACGCATTATGGTTTTGCCTGTGGTGTTCAACACTGTACCAGGTCACTGGAAAACCTAAAAGGCACAATCCATGAGAAAAATAAAAACATGGATTACTCAAAGGTGATGGCGTTCTAccatcca                                                                                                                    <  2:858417/149‑1 (MQ=255)

                                 aTAACGAAGAAGAAAAGGCACTGGCAACGCATTATGGTTTTGCCTGTGGTGTTCAACACTGTACCAGGTCACTGGAAAACCTAAAAGGCACAATCCATGAGAAAAATAAAAACATGGATTACTCAAAGGTGATGGCGTTCTAccatcca                                                                                                                    <  2:3842904/149‑1 (MQ=255)

                                   aaCGAAGAAGAAAAGGCACTGGCAACGCATTATGGTTTTGCCTGTGGTGTTCAACACTGTACCAGGTCACTGGAAAACCTAAAAGGCACAATCCATGAGAAAAATAAAAACATGGATTACTCAAAGGTGATGGCGTTCTACCATCCAtt                                                                                                                  >  2:2458204/1‑149 (MQ=255)

                                    aCGAAGAAGAAAAGGCACTGGCAACGCATTATGGTTTTGCCTGTGGTGTTCAACACTGTACCAGGTCACTGGAAAACCTAAAAGGCACAATCCATGAGAAAAATAAAAACATTGATTACTCAAAGGTGATGGCGTTCTACCATCCAttt                                                                                                                 <  2:458014/149‑1 (MQ=255)

                                    aCGAAGAAGAAAAGGCACTGGCAACGCATTATGGTTTTGCCTGTGGTGTTCAACACTGTACCAGGTCACTGGAAAACCTAAAAGGCACAATCCATGAGAAAAATAAAAACATGGATTACTCAAAGGTGATGGCGTTCTACCATCCAttt                                                                                                                 >  1:5031020/1‑149 (MQ=255)

                                    aCGAAGAAGAAAAGGCACTGGCAACGCATTATGGTTTTGCCTGTGGTGTTCAACACTGTACCAGGTCACTGGAAAACCTAAAAGGCACAATCCATGAGAAAAATAAAAACATGGATTACTCAAAGGTGATGGCGTTCTACCATCCAttt                                                                                                                 >  1:752753/1‑149 (MQ=255)

                                      gaagaagaaAAGGCACTGGCAACGCATTATGGTTTTGCCTGTGGTGTTCAACACTGTACCAGGTCACTGGAAAACCTAAAAGGCACAATCCATGAGAAAAATAAAAACATGGATTACTCAAAGGTGATGGCGTTCTACCATCCATTTaa                                                                                                               >  1:4490502/1‑149 (MQ=255)

                                       aagaagaaAAGGCACTGGCAACGCATTATGGTTTTGCCTGTGGTGTTCAACACTGTACCAGGTCACTGGAAAACCTAAAAGGCACAATCCATGAGAAAAATAAAAACATGGATTACTCAAAGGTGATGGCGTTCTACCATCCATTTaaa                                                                                                              <  2:2733974/149‑1 (MQ=255)

                                         gaagaaAAGGCACTGGCAACGCATTATGGTTTTGCCTGTGGTGTTCAACACTGTACCAGGTCACTGGAAAACCTAAAAGGCACAATCCATGAGAAAAATAAAAACATGGATTACTCAAAGGTGATGGCGTTCTACCATCCATTTAAAGt                                                                                                            <  2:3128972/149‑1 (MQ=255)

                                             aaaaGGCACTGGCAACGCATTATGGTTTTGCCTGTGGTGTTCAACACTGTACCAGGTCACTGGAAAACCTAAAAGGCACAATCCATGAGAAAAATAAAAACATGGATTACTCAAAGGTGATGGCGTTCTACCATCCATTTAAAGTGCGa                                                                                                        >  1:4934683/1‑149 (MQ=255)

                                             aaaaGGCACTGGCAACGCATTATGGTTTTGCCTGTGGTGTTCAACACTGTACCAGGTCACTGGAAAACCTAAAAGGCACAATCCATGAGAAAAATAAAAACATGGATTACTCAAAGGTGATGGCGTTCTACCATCCATTTAAAGTGCGa                                                                                                        <  1:4174273/149‑1 (MQ=255)

                                              aaaGGCACTGGCAACGCATTATGGTTTTGCCTGTGGTGTTCAACACTGTACCAGGTCACTGGAAAACCTAAAAGGCACAATCCATGAGAAAAATAAAAACATGGATTACTCAAAGGTGATGGCGTTCTACCATCCATTTAAAGTGCGAt                                                                                                       >  1:3424212/1‑149 (MQ=255)

                                              aaaGGCACTGGCAACGCATTATGGTTTTGCCTGTGGTGTTCAACACTGTACCAGGTCACTGGAAAACCTAAAAGGCACAATCCATGAGAAAAATAAAAACATGGATTACTCAAAGGTGATGGCGTTCTACCATCCATTTAAAGTGCGAt                                                                                                       >  1:1231833/1‑149 (MQ=255)

                                                 ggCACTGGCAACGCATTATGGTTTTGCCTGTGGTGTTCAACACTGTACCAGGTCACTGGAAAACCTACAAGGCACAATCCATGAGAAAAATAAAAACATTGATTACTCAAAGGTGATGGCGTTCTACCATCCATTTAAAGTGCGAtttt                                                                                                    <  2:3736118/149‑1 (MQ=255)

                                                 ggCACTGGCAACGCATTATGGTTTTGCCTGTGGTGTTCAACACTGTACCAGGTCACTGGAAAACCTAAAAGGCACAATCCATGAGAAAAATAAAAACATGGATTACTCAAAGGTGATGGCGTTCTACCATCCATTTAAAGTGCGAtttt                                                                                                    >  2:617278/1‑149 (MQ=255)

                                                  gCACTGGCAACGCATTATGGTTTTGCCTGTGGTGTTCAACACTGTACCAGGTCACTGGAAAACCTAAAAGGCACAATCCATGAGAAAAATAAAAACATGGATTACTCAAAGGTGATGGCGTTCTACCATCCATTTAAAGTGCGATTTTa                                                                                                   <  1:1859728/149‑1 (MQ=255)

                                                         cAACGCAGTATGGTTTTGCCTGTGGTGTTCAACACTGTACCAGGTCACTGGAAAACCTAAAAGGCACAATCCATGAGAAAAATAAAAACATGGATTACTCAAAGGTGATGGCGTTCTACCATCCATTTAAAGTGCGATTTTATGAATAc                                                                                            <  2:499693/149‑1 (MQ=255)

                                                          aaCGCATTATGGTTTTGCCTGTGGTGTTCAACACTGTACCAGGTCACTGGAAAACCTAAAAGGCACAATCCATGAGAAAAATAAAAACATGGATTACTCAAAGGTGATGGCGTTCTACCATCCATTTAAAGTGCGATTTTATGAATACt                                                                                           >  1:3103756/1‑149 (MQ=255)

                                                           aCGCATTATGGTTTTGCCTGTGGTGTTCAACACTGTACCAGGTCACTGGAAAACCTAAAAGGCACAATCCATGAGAAAAATAAAAACATGGATTACTCAAAGGTGATGGCGTTCTACCATCCATTTAAAGTGCGATTTTATGAATACTa                                                                                          <  1:1530668/149‑1 (MQ=255)

                                                           aCGCATTATGGTTTTGCCTGTGGTGTTCAACACTGTACCAGGTCACTGGAAAACCTAAAAGGCACAATCCATGAGAAAAATAAAAACATGGATTACTCAAAGGTGATGGCGTTCTACCATCCATTTAAAGTGCGATTTTATGAATACTa                                                                                          <  2:1884028/149‑1 (MQ=255)

                                                           aCGCATTATGGTTTTGCCTGTGGTGTTCAACACTGTACCAGGTCACTGGAAAACCTAAAAGGCACAATCCATGAGAAAAATAAAAACATGGATTACTCAAAGGTGATGGCGTTCTACCATCCATTTAAAGTGCGATTTTATGAATACTa                                                                                          <  1:1555251/149‑1 (MQ=255)

                                                              cATTATGGTTTTGCCTGTGGTGTTCAACACTGTACCAGGTCACTGGAAAACCTAAAAGGCACAATCCATGAGAAAAATAAAAACATGGATTACTCAAAGGTGATGGCGTTCTACCATCCATTTAAAGTGCGATTTTATGAATACTATTc                                                                                       <  1:4741855/149‑1 (MQ=255)

                                                              cATTATGGTTTTGCCTGTGGTGTTCAACACTGTACCAGGTCACTGGAAAACCTAAAAGGCACAATCCATGAGAAAAATAAAAACATGGATTACTCAAAGGTGATGGCGTTCTACCATCCATTTAAAGTGCGATTTTATGAATACTATTc                                                                                       <  1:4576561/149‑1 (MQ=255)

                                                              cATTATGGTTTTGCCTGTGGTGTTCAACACTGTACCAGGTCACTGGAAAACCTAAAAGGCACAATCCATGAGAAAAATAAAAACATGGATTACTCAAAGGTGATGGCGTTCTACCATCCATTTAAAGTGCGATTTTATGAATACTATTc                                                                                       <  1:1226514/149‑1 (MQ=255)

                                                               aTTATGGTTTTGCCTGTGGTGTTCAACACTGTACCAGGTCACTGGAAAACCTAAAAGGCACAATCCATGAGAAAAATAAAAACATGGATTACTCAAAGGTGATGGCGTTCTACCATCCATTTAAAGTGCGATTTTATGAATACTATTCa                                                                                      >  2:2219953/1‑149 (MQ=255)

                                                               aTTATGGTTTTGCCTGTGGTGTTCAACACTGTACCAGGTCACTGGAAAACCTAAAAGGCACAATCCATGAGAAAAATAAAAACATGGATTACTCAAAGGTGATGGCGTTCTACCATCCATTTAAAGTGCGATTTTATGAATACTATTCa                                                                                      >  2:5208383/1‑149 (MQ=255)

                                                                          gCCTGTGGTGTTCAACACTGTACCAGGTCACTGGAAAACCTAAAAGGCACAATCCATGAGAAAAATAAAAACATGGATTACTCAAAGGTGATGGCGTTCTACCATCCATTTAAAGTGCGATTTTATGAATACTATTCACCCAGAGGCAt                                                                           >  1:2274196/1‑149 (MQ=255)

                                                                            cTGTGGTGTTCAACACTGTACCAGGTCACTGGAAAACCTAAAAGGCACAATCCATGAGAAAAATAAAAACATGGATTACTCAAAGGTGATGGCGTTCTACCATCCATTTAAAGTGCGATTTTATGAATACTATTCACCCAGAGGCATTc                                                                         >  1:83993/1‑149 (MQ=255)

                                                                             tgtgGTGTTCAACACTGTACCAGGTCACTGGAAAACCTAAAAGGCACAATCCATGAGAAAAATAAAAACATGGATTACTCAAAGGTGATGGCGTTCTACCATCCATTTAAAGTGCGATTTTATGAATACTATTCACCCAGAGGCATTcc                                                                        <  1:2697093/149‑1 (MQ=255)

                                                                             tgtgGTGTTCAACACTGTACCAGGTCACTGGAAAACCTAAAAGGCACAATCCATGAGAAAAATAAAAACATGGATTACTCAAAGGTGATGGCGTTCTACCATCCATTTAAAGTGCGATTTTATGAATACTATTCACCCAGAGGCATTcc                                                                        >  1:621284/1‑149 (MQ=255)

                                                                                   gtTCAACACTGTACCAGGTCACTGGAAAACCTAAAAGGCACAATCCATGAGAAAAATAAAAACATGGATTACTCAAAGGTGATGGCGTTCTACCATCCATTTAAAGTGCGATTTTATGAATACTATTCACCCAGAGGCATTCCGGATgg                                                                  >  2:4446361/1‑149 (MQ=255)

                                                                                    ttCAACACTGTACCAGGTCACTGGAAAACCTAAAAGGCACAATCCATGAGAAAAATAAAAACATGGATTACTCAAAGGTGATGGCGTTCTACCATCCATTTAAAGTGCGATTTTATGAATACTATTCACCCAGAGGCATTCCGGATGgt                                                                 >  2:3335911/1‑149 (MQ=255)

                                                                                     tCAACACTGTACCAGGTCACTGGAAAACCTAAAAGGCACAATCCATGAGAAAAATAAAAACATTGATTACTCAAAGGTGATGGCGTTCTACCATCCATTTAAAGTGCGATTTTATGAATACTATTCACCCAGAGGCATTCCGGATGgtg                                                                <  1:439424/149‑1 (MQ=255)

                                                                                      cAACACTGTACCAGGTCACTGGAAAACCTAAAAGGCACAATCCATGAGAAAAATAAAAACATGGATTACTCAAAGGTGATGGCGTTCTACCATCCATTTAAAGTGCGATTTTATGAATACTATTCACCCAGAGGCATTCCGGATGgtgt                                                               >  1:826512/1‑149 (MQ=255)

                                                                                      cAACACTGTACCAGGTCACTGGAAAACCTAAAAGGCACAATCCATGAGAAAAATAAAAACATGGATTACTCAAAGGTGATGGCGTTCTACCATCCATTTAAAGTGCGATTTTATGAATACTATTCACCCAGAGGCATTCCGGATGgtgt                                                               >  1:983645/1‑149 (MQ=255)

                                                                                       aaCACTGTACCAGGTCACTGGAAAACCTAAAAGGCACAATCCATGAGAAAAATAAAAACATGGATTACTCAAAGGTGATGGCGTTCTACCATCCATTTAAAGTGCGATTTTATGAATACTATTCACCCAGAGGCATTCCGGATGGTGtt                                                              <  2:1999680/149‑1 (MQ=255)

                                                                                           cTGTACCAGGTCACTGGAAAACCTAAAAGGCACAATCCATGAGAAAAATAAAAACATGGATTACTCAAAGGTGATGGCGTTCTACCATCCATTTAAAGTGCGATTTTATGAATACTATTCACCCAGAGGCATTCCGGATGGTGTTTCCg                                                          >  1:728859/1‑149 (MQ=255)

                                                                                            tGTACCAGGTCACTGTAAAACCTAAAAGGCACTATCCATGATAATAATATTAACCTGTTTTACTCAAATGTGATGGAGTTCTAACTTACATTTAAATTGCTATTTTATGAATACTATTCTCCCAGAGGCATTCCGGATGGTGTTTcctc                                                         <  1:1667859/149‑3 (MQ=255)

                                                                                                  aGGTCACTGGAAAACCTAAAAGGCACAATCCATGAGAAAAATAAAAACATGGATTACTCAAAGGTGATGGCGTTCTACCATCCATTTAAAGTGCGATTTTATGAATACTATTCACCCAGAGGCATTCCGGATGGTGTTTCCGCAGCAtt                                                   <  1:303002/149‑1 (MQ=255)

                                                                                                   ggTCACTGGAAAACCTAAAAGGCACAATCCATGAGAAAAATAAAAACATGGATTACTCAAAGGTGATGGCGTTCTACCATCCATTTAAAGTGCGATTTTATGAATACTATTCACCCAGAGGCATTCCGGATGGTGTTTCCGCAGCATTa                                                  >  1:1946211/1‑149 (MQ=255)

                                                                                                       aCTGGAAAACCTAAAAGGCACAATCCATGAGAAAAATAAAAACATGGATTACTCAAAGGTGATGGCGTTCTACCATCCATTTAAAGTGCGATTTTATGAATACTATTCACCCAGAGGCATTCCGGATGGTGTTTCCGCAGCATTACTGc                                              <  1:3273952/149‑1 (MQ=255)

                                                                                                       aCTGGAAAACCTAAAAGGCACAATCCATGAGAAAAATAAAAACATGGATTACTCAAAGGTGATGGCGTTCTACCATCCATTTAAAGTGCGATTTTATGAATACTATTCACCCAGAGGCATTCCGGATGGTGTTTCCGCAGCATTACTGc                                              >  2:574570/1‑149 (MQ=255)

                                                                                                        cTGGAAAACCTAAAAGGCACAATCCATGAGAAAAATAAAAACATGGATTACTCAAAGGTGATGGCGTTCTACCATCCATTTAAAGTGCGATTTTATGAATACTATTCACCCAGAGGCATTCCGGATGGTGTTTCCGCAGCATTACTGcc                                             <  1:5212179/149‑1 (MQ=255)

                                                                                                         tGGAAAACCTAAAAGGCACAATCCATGAGAAAAATAAAAACATTGATTACTCAAAGGTGATGGCGTTCTACCATCCATTTAAAGTGCGATTTTATGAATACTATTCACCCAGAGGCATTCCGGATGGTGTTTCCGCAGCATTACTGCCa                                            >  2:171182/1‑149 (MQ=255)

                                                                                                         tGGAAAACCTAAAAGGCACAATCCATGAGAAAAATAAAAACATGGATTACTCAAAGGTGATGGCGTTCTACCATCCATTTAAAGTGCGATTTTATGAATACTATTCACCCAGAGGCATTCCGGATGGTGTTTCCGCAGCATTACTGCCa                                            <  2:4648899/149‑1 (MQ=255)

                                                                                                          ggAAAACCTAAAAGGCACAATCCATGAGAAAAATAAAAACATGGATTACTCAAAGGTGATGGCGTTCTACCATCCATTTAAAGTGCGATTTTATGAATACTATTCACCCAGAGGCATTCCGGATGGTGTTTCCGCAGCATTACTGCCAg                                           <  2:2521144/149‑1 (MQ=255)

                                                                                                          ggAAAACCTAAAAGGCACAATCCATGAGAAAAATAAAAACATGGATTACTCAAAGGTGATGGCGTTCTACCATCCATTTAAAGTGCGATTTTATGAATACTATTCACCCAGAGGCATTCCGGATGGTGTTTCCGCAGCATTACTGCCAg                                           <  2:738277/149‑1 (MQ=255)

                                                                                                              aaCCTAAAAGGCACAATCCATGAGAAAAATAAAAACATGGATTACTCAAAGGTGATGGCGTTCTACCATCCATTTAAAGTGCGATTTTATGAATACTATTCACCCAGAGGCATTCCGGATGGTGTTTCCGCAGCATTACTGCCAGTGAc                                       >  1:2896584/1‑149 (MQ=255)

                                                                                                              aaCCTAAAAGGCACAATCCATGAGAAAAATAAAAACATGGATTACTCAAAGGTGATGGCGTTCTACCATCCATTTAAAGTGCGATTTTATGAATACTATTCACCCAGAGGCATTCCGGATGGTGTTTCCGCAGCATTACTGCCAGTGAc                                       <  1:1625918/149‑1 (MQ=255)

                                                                                                                ccTAAAAGGCACAATCCATGAGAAAAATAAAAACATGGATTACTCAAAGGTGATGGCGTTCTACCATCCATTTAAAGTGCGATTTTATGAATACTATTCACCCAGAGGCATTCCGGATGGTGTTTCCGCAGCATTCCTGCCAGTGACTg                                     >  1:3998427/1‑149 (MQ=255)

                                                                                                                ccTAAAAGGCACAATCCATGAGAAAAATAAAAACATGGATTACTCAAAGGTGATGGCGTTCTACCATCCATTTAAAGTGCGATTTTATGAATACTATTCACCCAGAGGCATTCCGGATGGTGTTTCCGCAGCATTACTGCCAGTGACTg                                     >  1:1851331/1‑149 (MQ=255)

                                                                                                                  tAAAAGGCACAATCCATGAGAAAAATAAAAACATGGATTACTCAAAGGTGATGGCGTTCTACCATCCATTTAAAGTGCGATTTTATGAATACTATTCACCCAGAGGCATTCCGGATGGTGTTTCCGCAGCATTACTGCCAGTGACTGtt                                   <  2:3700792/149‑1 (MQ=255)

                                                                                                                  tAAAAGGCACAATCCATGAGAAAAATAAAAACATGGATTACTCAAAGGTGATGGCGTTCTACCATCCATTTAAAGTGCGATTTTATGAATACTATTCACCCAGAGGCATTCCGGATGGTGTTTCCGCAGCATTACTGCCAGTGACTGtt                                   >  2:5078268/1‑149 (MQ=255)

                                                                                                                  tAAAAGGCACAATCCATGAGAAAAATAAAAACATGGATTACTCAAAGGTGATGGCGTTCTACCATCCATTTAAAGTGCGATTTTATGAATACTATTCACCCAGAGGCATTCCGGATGGTGTTTCCGCAGCATTACTGCCAGTGACTGtt                                   <  2:4366531/149‑1 (MQ=255)

                                                                                                                   aaaaGGCACAATCCATGAGAAAAATAAAAACATGGATTACTCAAAGGTGATGGCGTTCTACCATCCATTTAAAGTGCGATTTTATGAATACTATTCACCCAGAGGCATTCCGGATGGTGTTTCCGCAGCATTACTGCCAGTGACTGTTa                                  <  2:3282264/149‑1 (MQ=255)

                                                                                                                   aaaaGGCACAATCCATGAGAAAAATAAAAACATGGATTACTCAAAGGTGATGGCGTTCTACCATCCATTTAAAGTGCGATTTTATGAATACTATTCACCCAGAGGCATTCCGGATGGTGTTTCCGCAGCATTACTGCCAGTGACTGTTa                                  >  2:292925/1‑149 (MQ=255)

                                                                                                                       ggCACAATCCATGAGAAAAATAAAAACATGGATTACTCAAAGGTGATGGCGTTCTACCATCCATTTAAAGTGCGATTTTATGAATACTATTCACCCAGAGGCATTCCGGATGGTGTTTCCGCAGCATTACTGCCAGTGACTGTTACGCt                              <  2:1060057/149‑1 (MQ=255)

                                                                                                                          acaATCCATGAGAAAAATAAAAACATGGATTACTCAAAGGTGATGGCGTTCTACCATCCATTTAAAGTGCGATTTTATGAATACTATTCACCCAGAGGCATTCCGGATGGTGTTTCCGCAGCATTACTGCCAGTGACTGTTACGCTGGa                           <  1:4688489/149‑1 (MQ=255)

                                                                                                                           caATCCATGAGAAAAATAAAAACATGGATTACTCAAAGGTGATGGCGTTCTACCATCCATTTAAAGTGCGATTTTATGAATACTATTCACCCAGAGGCATTCCGGATGGTGTTTCCGCAGCATTACTGCCAGTGACTGTTACGCTGGAc                          >  1:3171629/1‑149 (MQ=255)

                                                                                                                           caATCCATGAGAAAAATAAAAACATGGATTACTCAAAGGTGATGGCGTTCTACCATCCATTTAAAGTGCGATTTTATGAATACTATTCACCCAGAGGCATTCCGGATGGTGTTTCCGCAGCATTACTGCCAGTGACTGTTACGCTGGAc                          <  1:1372837/149‑1 (MQ=255)

                                                                                                                           caATCCATGAGAAAAATAAAAACATGGATTACTCAAAGGTGATGGCGTTCTACCATCCATTTAAAGTGCGATTTTATGAATACTATTCACCCAGAGGCATTCCGGATGGTGTTTCAGCAGCATTACTGCCAGTGACTGTTACGCTGGAc                          >  1:2216947/1‑149 (MQ=255)

                                                                                                                             aTCCATGAGAAAAATAAAAACATTGATTACTCAAAGGTGATGGCGTTCTACCATCCATTTAAAGTGCGATTTTATGAATACTATTCACCCAGAGGCATTCCGGATGGTGTTTCCGCAGCATTACTGCCAGTGACTGTTACGCTGGAcat                        >  2:2217843/1‑149 (MQ=255)

                                                                                                                              tCCATGAGAAAAATAAAAACATGGATTACTCAAAGGTGATGGCGTTCTACCATCCATTTAAAGTGCGATTTTATGAATACTATTCACCCAGAGGCATTCCGGATGGTGTTTCCGCAGCATTACTGCCAGTGACTGTTACGCTGGAcatc                       >  2:2532775/1‑149 (MQ=255)

                                                                                                                                cATGAGAAAAATAAAAACATGGATTACTCAAAGGTGATGGCGTTCTACCATCCATTTAAAGTGCGATTTTATGAATACTATTCACCCAGAGGCATTCCGGATGGTGTTTCCGCAGCATTACTGCCAGTGACTGTTACGCTGGAcatcat                     >  2:3373548/1‑149 (MQ=255)

                                                                                                                                cATGAGAAAAATAAAAACATGGATTACTCAAAGGTGATGGCGTTCTACCATCCATTTAAAGTGCGATTTTATGAATACTATTCACCCAGAGGCATTCCGGATGGTGTTTCCGCAGCATTACTGCCAGTGACTGTTACGCTGGAcatcat                     <  1:1287368/149‑1 (MQ=255)

                                                                                                                                   gagaAAAATAAAAACATGGATTACTCAAAGGTGATGGCGTTCTACCATCCATTTAAAGTGCGATTTTATGAATACTATTCACCCAGAGGCATTCCGGATGGTGTTTCCGCAGCATTACTGCCAGTGACTGTTACGCTGGACATCATTAc                  <  2:1758260/149‑1 (MQ=255)

                                                                                                                                    agaAAAATAAAAACATGGATTACTCAAAGGTGATGGCGTTCTACCATCCATTTAAAGTGCGATTTTATGAATACTATTCACCCAGAGGCATTCCGGATGGTGTTTCCGCAGCATTACTGCCAGTGACTGTTACGCTGGACATCATTACt                 >  1:4852123/1‑149 (MQ=255)

                                                                                                                                     gaAAAATAAAAACATGGATTACTCAAAGGTGATGGCGTTCTACCATCCATTTAAAGTGCGATTTTATGAATACTATTCACCCAGAGGCATTCCGGATGGTGTTTCCGCAGCATTACTGCCAGTGACTGTTACGCTGGACATCATTACTg                >  2:914693/1‑149 (MQ=255)

                                                                                                                                     gaAAAATAAAAACATGGATTACTCAAAGGTGATGGCGTTCTACCATCCATTTAAAGTGCGATTTTATGAATACTATTCACCCAGAGGCATTCCGGATGGTGTTTCCGCAGCATTACTGCCAGTGACTGTTACGCTGGACATCATTACTg                <  1:2006492/149‑1 (MQ=255)

                                                                                                                                      aaaaaTAAAAACATGGATTACTCAAAGGTGATGGCGTTCTACCATCCATTTAAAGTGCGATTTTATGAATACTATTCACCCAGAGGCATTCCGGATGGTGTTTCCGCAGCATTACTGCCAGTGACTGTTACGCTGGACATCATTACTGc               >  2:1550556/1‑149 (MQ=255)

                                                                                                                                       aaaaTAAAAACATGGATTACTCAAAGGTGATGGCGTTCTACCATCCATTTAAAGTGCGATTTTATGAATACTATTCACCCAGAGGCATTCCGGATGGTGTTTCCGCAGCATTACTGCCAGTGACTGTTACGCTGGACATCATTACTGCa              >  1:3528959/1‑149 (MQ=255)

                                                                                                                                        aaaTAAAAACATGGATTACTCAAAGGTGATGGCGTTCTACCATCCATTTAAAGTGCGATTTTATGAATACTATTCACCCAGAGGCATTCCGGATGGTGTTTCCGCAGCATTACTGCCAGTGACTGTTACGCTGGACATCATTACTGCAc             >  1:4793716/1‑149 (MQ=255)

                                                                                                                                            aaaaaCATGGATTACTCAAAGGTGATGGCGTTCTACCATCCATTTAAAGTGCGATTTTATGAATACTATTCACCCAGAGGCATTCCGGATGGTGTTTCCGCAGCATTACTGCCAGTGACTGTTACGCTGGACATCATTACTGCACCGCt         <  2:3777703/149‑1 (MQ=255)

                                                                                                                                             aaaaCATGGATTACTCAAAGGTGATGGCGTTCTACCATCCATTTAAAGTGCGATTTTATGAATACTATTCACCCAGAGGCATTCCGGATGGTGTTTCCGCAGCATTACTGCCAGTGACTGTTACGCTGGACATCATTACTGCACCGCTg        <  1:611950/149‑1 (MQ=255)

                                                                                                                                             aaaaCATGGATTACTCAAAGGTGATGGCGTTCTACCATCCATTTAAAGTGCGATTTTATGAATACTATTCACCCAGAGGCATTCCGGATGGTGTTTCCGCAGCATTACTGCCAGTGACTGTTACGCTGGACATCATTACTGCACCGCTg        <  1:5110635/149‑1 (MQ=255)

                                                                                                                                             aaaaCATGGATTACTCAAAGGTGATGGCGTTCTACCATCCATTTAAAGTGCGATTTTATGAATACTATTCACCCAGAGGCATTCCGGATGGTGTTTCCGCAGCATTACTGCCAGTGACTGTTACGCTGGACATCATTACTGCACCGCTg        >  1:4041070/1‑149 (MQ=255)

                                                                                                                                             aaaaCATGGATTACTCAAAGGTGATGGCGTTCTACCATCCATTTAAAGTGCGATTTTATGAATACTATTCACCCAGAGGCATTCCGGATGGTGTTTCCGCAGCATTACTGCCAGTGACTGTTACGCTGGACATCATTACTGCACCGCTg        >  1:2376041/1‑149 (MQ=255)

                                                                                                                                               aaCATGGATTACTCAAAGGTGATGGCGTTCTACCATCCATTTAAAGTGCGATTTTATGAATACTATTCACCCAGAGGCATTCCGGATGGTGTTTCCGCAGCATTACTGCCAGTGACTGTTACGCTGGACATCATTACTGCACCGCTGCa      >  1:3765349/1‑149 (MQ=255)

                                                                                                                                               aaCATGGATTACTCAAAGGTGATGGCGTTCTACCATCCATTTAAAGTGCGATTTTATGAATACTATTCACCCAGAGGCATTCCGGAAGGTGTTTCCGCAGCATTACTGCCAGTGACTGTTACGCTGGACATCATTACTGCACCGCTGCa      >  1:384207/1‑149 (MQ=255)

                                                                                                                                                aCATGGATTACTCAAAGGTGATGGCGTTCTACCATCCATTTAAAGTGCGATTTTATGAATACTATTCACCCAGAGGCATTCCGGATGGTGTTTCCGCAGCATTACTGCCAGTGACTGTTACGCTGGACATCATTACTGCACCGCTGCaa     <  1:1750461/149‑1 (MQ=255)

TTACCGGCACAATATCGCTTTACTACTACTGGAATAACGAAGAAGAAAAGGCACTGGCAACGCATTATGGTTTTGCCTGTGGTGTTCAACACTGTACCAGGTCACTGGAAAACCTAAAAGGCACAATCCATGAGAAAAATAAAAACATGGATTACTCAAAGGTGATGGCGTTCTACCATCCATTTAAAGTGCGATTTTATGAATACTATTCACCCAGAGGCATTCCGGATGGTGTTTCCGCAGCATTACTGCCAGTGACTGTTACGCTGGACATCATTACTGCACCGCTGCAATTT  >  CP009273/3869132‑3869427

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`