Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,926,609 C→A 5.5% intergenic (+65/‑102) kup → / → rbsD potassium transporter/putative cytoplasmic sugar‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,926,6090CA 5.5% 954.5 / 12.4 273intergenic (+65/‑102)kup/rbsDpotassium transporter/putative cytoplasmic sugar‑binding protein
Reads supporting (aligned to +/- strand):  ref base C (93/155);  new base A (22/2);  total (115/158)
Fisher's exact test for biased strand distribution p-value = 1.90e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 280 total aligned reads displayed.

CAACGTAATGCGCTGCGTGCACCAGATCAATTTGAAATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGTTTTTGTTTTCATGGTTAATCACCATGTAAAACGTTTCGAGGTTGATCACATTTCCGTAACGTCACGATGGTTTTCCCAACTCAGTCAGGATTAAACTGTGGGTCAGCGAAACGTTTCGCTGATGGAGAAAAAAATGAAAAAAGGCACCGTTCTTAATTCTGATATTTCATCGGTGATCTC  >  CP009273/3926461‑3926757
                                                                                                                                                    |                                                                                                                                                    
cAACGTAATGCGCTGCGTGCACCAGATCAATTTGACATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGTTTTTGTTTTCATGGTTAATCACCATGTAAAAc                                                                                                                                                      <  1:3221093/149‑1 (MQ=255)
cAACGTAATGCGCTGCGTGCACCAGATCAATTTGACATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGTTTTTGTTTTCATGGTTAATCACCATGTAAAAc                                                                                                                                                      <  1:4272199/149‑1 (MQ=255)
  aCGTAATGCGCTGCGTGCACCAGATCAATTTGAAATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGTTTTTGTTTTCATGGTTAATCACCATGTAAAACGt                                                                                                                                                    >  1:1057053/1‑149 (MQ=255)
    gTAATGCGCTGCGTGCACCAGATCAATTTGAAATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGTTTTTGTTTTCATGGTTAATCACCATGTAAAACGttt                                                                                                                                                  <  2:2301255/149‑1 (MQ=255)
     tAATGCGCTGCGTGCACCAGATCAATTTGAAATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGTTTTTGTTTTCATGGTTAATCACCATGTAAAACGTTTc                                                                                                                                                 <  1:782496/149‑1 (MQ=255)
      aaTGCGCTGCGTGCACCAGATCAATTTGAAATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGTTTTTTTTTTAATTGTTAATCACCATGTAAAACGTTTCg                                                                                                                                                >  1:1714258/1‑149 (MQ=255)
       aTGCGCTGCGTGCACCAGATCAATTTGAAATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGTTTTTGTTTTCATGGTTAATCACCATGTAAAACGTTTCGa                                                                                                                                               <  2:653268/149‑1 (MQ=255)
       aTGCGCTGCGTGCACCAGATCAATTTGAAATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGTTTTTGTTTTCATGGTTAATCACCATGTAAAACGTTTCGa                                                                                                                                               <  1:2240479/149‑1 (MQ=255)
        tGCGCTGCGTGCACCAGATCAATTTGAAATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGTTTTTGTTTTCATGGTTAATCACCATGTAAAACGTTTCGAg                                                                                                                                              <  1:4602949/149‑1 (MQ=255)
         gcgcTGCGTGCACCAGATCAATTTGAAATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGTTTTTGTTTTCATGGTTAATCACCATGTAAAACGTTTCGAgg                                                                                                                                             <  1:826876/149‑1 (MQ=255)
          cgcTGCGTGCACCAGATCAATTTGAAATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGTTTTTGTTTTCATGGTTAATCACCATGTAAAACGTTTCGAGGt                                                                                                                                            <  1:2582639/149‑1 (MQ=255)
           gcTGCGTGCACCAGATCAATTTGAAATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGTTTTTGTTTTCATGGTTAATCACCATGTAAAACGTTTCGAGGtt                                                                                                                                           <  1:1090735/149‑1 (MQ=255)
           gcTGCGTGCACCAGATCAATTTGAAATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGTTTTTGTTTTCATGGTTAATCACCATGTAAAACGTTTCGAGGtt                                                                                                                                           <  2:5072171/149‑1 (MQ=255)
                  gCACCAGATCAATTTGAAATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGTTTTTGTTTTCATGGTTAATCACCATGTAAAACGTTTCGAGGTTGATcaca                                                                                                                                    <  2:4127863/149‑1 (MQ=255)
                       aGATCAATTTGAAATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGTTTTTGTTTTCATGGTTAATCACCATGTAAAACGTTTCGAGGTTGATCACATTTcc                                                                                                                               <  2:5197845/149‑1 (MQ=255)
                       aGATCAATTTGAAATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGTTTTTGTTTTCATGGTTAATCACCATGTAAAAAGTTTCGAGGTTGATCACATTTcc                                                                                                                               >  2:4208109/1‑149 (MQ=255)
                        gATCAATTTGAAATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGTTTTTGTTTTCATGGTTAATCACCATGTAAAACGTTTCGAGGTTGATCACATTTCCg                                                                                                                              <  2:2878650/149‑1 (MQ=255)
                            aaTTTGAAATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGTTTTTGTTTTCATGGTTAATCACCATGTAAAACGTTTCGAGGTTGATCACATTTCCGTAAc                                                                                                                          >  1:855170/1‑149 (MQ=255)
                             aTTTGAAATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGTTTTTGTTTTCATGGTTAATCACCATGTAAAACGTTTCGAGGTTGATCACATTTCCGTAACg                                                                                                                         <  1:5133872/149‑1 (MQ=255)
                                   aaTCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGTTTTTGTTTTCATGGTTAATCACCATGTAAAACGTTTCGAGGTTGATCACATTTCCGTAACGTCACGa                                                                                                                   <  1:4989611/149‑1 (MQ=255)
                                    aTCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGTTTTTGTTTTCATGGTTAATCACCATGTAAAACGTTTCGAGGTTGATCACATTTCCGTAACGTCACGAt                                                                                                                  <  1:2152871/149‑1 (MQ=255)
                                    aTCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGTTTTTGTTTTCATGGTTAATCACCATGTAAAACGTTTCGAGGTTGATCACATTTCCGTAACGTCACGAt                                                                                                                  <  2:3846256/149‑1 (MQ=255)
                                     tCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGTTTTTGTTTTCATGGTTAATCACCATGTAAAACGTTTCGAGGTTGATCACATTTCCGTAACGTCACGATg                                                                                                                 >  2:1852433/1‑149 (MQ=255)
                                     tCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGTTTTTGTTTTCATGGGTAATCACCATGTAAAACGTTTCGAGGGTGATCACATTTCCGTAACGTCACGATg                                                                                                                 >  2:1895386/1‑149 (MQ=255)
                                         gCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGTGTTTGTTTTCATGGTTAATCACCATGTAAAACGTTTAGAGGTTGATCACATTTACGGAACGTCACGATGGgtt                                                                                                             >  2:1323800/1‑149 (MQ=255)
                                          ccAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGTTTTTGTTTTCATGGTTAATCACCATGTAAAACGTTTCGAGGTTGATCACATGTCCGTAACGTCACGATGGtttt                                                                                                            <  1:1326226/149‑1 (MQ=255)
                                           cAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGTTTTTGTTTTCATGGTTAATCACCATGTAAAACGTTTCGAGGTTGATCACATTTCCGTAACGTCACGATGGTTTTc                                                                                                           <  1:2086552/149‑1 (MQ=255)
                                                aGGGTTATCGAACTGGGTACTCAGGTCTAAATCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGTTTTTGTTTTCATGGTTAATCACCATGTAAAACGTTTCGAGGTTGATCACATTTCCGTAACGTCACGATGGTTTTCCCAAc                                                                                                      <  1:1915076/149‑1 (MQ=255)
                                                aGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGTTTTTGTTTTCATGGTTAATCACCATGTAAAACGTTTCGAGGTTGATCACATTTCCGTAACGTCACGATGGTTTTCCCAAc                                                                                                      <  1:1914978/149‑1 (MQ=255)
                                                 gggTTATCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGTTTTTGTTTTCATGGTTAATCACCATGTAAAACGTTTCGAGGTTGATCACATTTCCGTAACGTCACGATGGTTTTCCCAACt                                                                                                     >  2:1400923/1‑149 (MQ=255)
                                                  ggTTATCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGTTTTTGTTTTCATGGTTAATCACCATGTAAAACGTTTCGAGGTTGATCACATTTCCGTAACGTCACGATGGTTTTCCCAACtc                                                                                                    >  2:614067/1‑149 (MQ=255)
                                                   gTTATCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGTTTTTGTTTTCATGGTTAATCACCATGTAAAACGTTTCGAGGTTGATCACATTTCCGTAACGTCACGATGGTTTTCCCAACtca                                                                                                   <  1:142420/149‑1 (MQ=255)
                                                      aTCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGTTTTTTTTTTCATGGTTAATAACCAAGTAAAAAGTTTCGAGGTTGATAACATTTCCGTAACGTCACGATGGTTTTACCAACacagtc                                                                                                >  1:4231918/1‑149 (MQ=255)
                                                      aTCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGTTTTTGTTTTCATGGTTAATCACCATGTAAAACGTTTAGAGGTTGATAACATTTCCGTAACGTCACGATGGTTTTCCCAACtcatta                                                                                                >  1:3721708/1‑146 (MQ=255)
                                                       tCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGTTTTTGTTTTCATGGTTAATCACCATGTAAAACGTTTCGAGGTTGATCACATTTCCGTAACGTCACGATGGTTTTCCCAACtcagtca                                                                                               <  1:2151086/149‑1 (MQ=255)
                                                         gAACTGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGTTTTTGTTTTCATGGTTAATCACCATGTAAAACGTTTCGAGGTTGATCACATTTCCGTAACGTCACTATGGTTTTCCCAACTCAGTCAgg                                                                                             >  2:1185739/1‑149 (MQ=255)
                                                          aaCTGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGTTTTTTTTTTCATGGTTAATAACCAAGTAAAACGTTTCGAGGTTGATCACATTTCCGGAACGTCACGATGGTTTTACCAACTCAGTCAGGa                                                                                            >  1:1067822/1‑149 (MQ=255)
                                                          aaCTGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGTTTTTGTTTTCATGGTTAATCACCATGTAAAACGTTTCGAGGTTGATCACATTTCCGTAACGTCACGATGTTTTTCCCAACTCAGTCAGGa                                                                                            <  2:1641652/149‑1 (MQ=255)
                                                          aaCTGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGTTTTTGTTTTCATGGTTAATCACCATGTAAAACGTTTCGAGGTTGATCACATTTCCGTAACGTCACGATGGTTTTCCCAACTCAGTCAGGa                                                                                            <  1:4445431/149‑1 (MQ=255)
                                                          aaCTGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGTTTTTGTTTTCATGGTTAATCACCATGTAAAACGTTTCGAGGTTGATCACATTTCCGTAACGTCACGATGGTTTTCCCAACTCAGTCAGGa                                                                                            <  2:4258189/149‑1 (MQ=255)
                                                          aaCTGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGTTTTTGTTTTCATGGGTAATCACCATGTAAAACGTTTAGAGGTTGATCACCTTTCCGGAACGTCACGATGGTTTTACCCACTCAGTCAggc                                                                                            >  1:330497/1‑148 (MQ=255)
                                                          aaCTGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGTTTTTGTTTTAATGGTTAATCACCATTTAAAAAGTTTCGAGGTTGATAACATTTCCGTAACGTCACGATGGTTTTACCAACTCAGTCAGGa                                                                                            >  1:5128820/1‑149 (MQ=255)
                                                           aCTGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGTTTTTTTTTTAATGGTTAAACACCCTGTAAAACCTTTAGAGGTTGTTCACATTTACGTAACGTCACGATGGTTTTACCAACACAGTCAGGAt                                                                                           >  1:3597374/1‑149 (MQ=255)
                                                            cTGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGTGTTTGTTTTCATGGTTAATCACCATGTAAAACGTTTCGAGGTTGATCACATTTCCGTAACGTCACGATGGTTTTCCCAACTCAGTCAGGAtt                                                                                          >  1:1761389/1‑149 (MQ=255)
                                                             tGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGTTTTTGTTTTCATGGTTAATCACCATGTAAAACGTTTCGAGGTTGATCACATTTCCGTAACGTCACGATGGTTTTCCCAACTCAGTCAGGATTa                                                                                         <  1:4759863/149‑1 (MQ=255)
                                                              ggttACTCAGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGTTTTTGTTTTCATGGTTAATCACCATGTATAACGTTTCGAGGTTGATCACATTTCCGTAACGTCACGATGGTTTTCCCAACTCAGTCAGGATTaa                                                                                        <  1:1933021/146‑1 (MQ=255)
                                                              gggTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGTTTTTTTTTTCATGGTTAATCACCATGTAAAACGTTTCGAGGTTGATCACATTTCCGTAACGTCACGATGGTTTTCCCAACTCAGTCAGGATTaa                                                                                        >  2:4410672/1‑149 (MQ=255)
                                                               ggTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGTTTTTGTTTTCATGGTTAATCACCATGTAAAACGTTTCGAGGTTGATCACATTTCCGTAACGTCACGATGGTTTTCCCAACTCAGTCAGGATTaaa                                                                                       >  2:1925482/1‑149 (MQ=255)
                                                                   cTCAGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGTTTTTGTTTTCATGGTTAATCACCATGTAAAACGTTTCGAGGTTGATCACATTTCCGTAACGTCACGATGGTTTTCCCAACTCAGTCAGGATTAAACtgt                                                                                   <  1:2502501/149‑1 (MQ=255)
                                                                        gTCGAAATCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGTTTTTGTTTTCATGGTTAATCACCATGTAAAACGTTTCGAGGTTGATCACATTTCCGTAACGTCACGATGGTTTTCCCAACTCAGTCAGGATTAAACTGTGGGTc                                                                              <  1:761473/149‑1 (MQ=255)
                                                                            aaaTCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGTTTTTTTTTTCAAGGTTAATAAAAATTTAAAAAGTTTATATGGTTATAACAATTAAGTAAAGTAACGAAGTTTTTACAAACACAGTCAGGATTAAACAGTTGGtgatcg                                                                          >  1:1858202/1‑144 (MQ=255)
                                                                            aaaTCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGTTTTTGTTTTCATGGTTAATCACCATGTAAAACGTTTCGAGGTTGATCACATTTCCGTAACGTCACGATGGTTTTCCCAACTCAGTCAGGATTAAACTGTGGGTCAGCg                                                                          >  2:3743204/1‑149 (MQ=255)
                                                                            aaaTCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGTTTTTGTTTTAATTGTTAATAACCATGTAAAACGTTTCGAGGTTGATCACATTTCCGTAACGTCACGATGGTTTTGCCAACTCAGTCAGGATTAAAATGTGGGTCAggg                                                                          >  1:2418613/1‑147 (MQ=255)
                                                                             aaTCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGTTTTTGTTTTCATGGTTAATCACCATGTAAAACGTTTCGAGGTTGATCACATTTCCGTAACGTCACGATGGTTTTCCCAACTCAGTCAGGATTAAACTGTGGGTCAGCGa                                                                         <  2:459394/149‑1 (MQ=255)
                                                                                cTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGTTTTTGTTTTCATGGTTAATCACCATGTAAAACGTTTCGAGGTTGATCACATTTCCGTAACGTCACGATGGTTTTCCCAACTCAGTCAGGATTAAACTGTGGGTCAGCGAAAc                                                                      <  2:3147441/149‑1 (MQ=255)
                                                                                    cGCCAGACGCCTCCTTTCTTCATAAGGGGGCGTTTTTGTTTTCATGGTTAATCACCATGTAAAACGTTTCGAGGTTGATCACATTTCCGTAACGTCACGATGGTTTTCCCAACTCAGTCAGGATTAAACTGTGGGTCAGCGAAACGttt                                                                  <  1:3544282/149‑1 (MQ=255)
                                                                                     gCCAGACGCCTCCTTTCTTCATAAGGGGGCGTTTTTTTTTTCATTGTTAATAAACATGTAAAACGTTTAGAGGTTGTTCACATTTCCGTAACGTCACGTTGGTTTTCCCAACTCAGTCAGGATTAAACTGTGGGGCAGGGCAAAGttta                                                                 >  1:2148276/1‑148 (MQ=255)
                                                                                     gCCAGACGCCTCCTTTCTTCATAAGGGGGCGTTTTTGTTTTCATGGTTAATCACCATGTAAAACGTTTCGAGGTTGATCACATTTCCGTAACGTCACGATGGTTTTCCCAACTCAGTCAGGATTAAACTGTGGGTCAGCGAAACGTTTc                                                                 <  1:2503454/149‑1 (MQ=255)
                                                                                        aGACGCCTCCTTTCTTCATAAGGGGGCGTTTTTGTTTTCATGGTTAATCACCATGTAAAACGTTTCGAGGTTGATCACATTTCCGTAACGTCACGATGGTTTTCCCAACTCAGTCAGGATTAAACTGTGGGTCAGCGAAACGTTTCGCt                                                              <  1:3037711/149‑1 (MQ=255)
                                                                                         gACGCCTCCTTTCTTCATAAGGGGGCGTTTTTGTTTTCATGGTTAATCACCATGTAAAACGTTTCGAGGTTGATCACATTTCCGTAACGTCACGATGGTTTTCCCAACTCAGTCAGGATTAAACTGTGGGTCAGCGAAACGTTTCGCTg                                                             <  1:4136951/149‑1 (MQ=255)
                                                                                         gACGCCTCCTTTCTTCATAAGGGGGCGTTTTTGTTTTCATGGTTAATCACCATGTAAAACGTTTCGAGGTTGATCACATTTCCGTAACGTCACGATGGTTTTCCCAACTCAGTCAGGATTAAACTGTGGGTCAGCGAAAAGTTTAGcag                                                             >  2:2665110/1‑147 (MQ=255)
                                                                                         gACGCCTCCTTTCTTCATAAGGGGGCGTTTTTGTTTTCATGGTTAATCACCATGTAAAAAGTTTCGAGGTTGATCACATTTCCGTAACGTCACGATGGTTTTCCCAACTCAGTCAGGATTAAACTGTGGGTCAGCGAAACGTTTCGCTg                                                             <  1:4208125/149‑1 (MQ=255)
                                                                                          aCGCCTCCTTTCTTCATAAGGGGGCGTTTTTGTTTTCATGGTTAATCACCATGTAAAACGTTTCGAGGTTGATCACATTTCCGTAACGTCACGATGGTTTTCCCAACTCAGTCAGGATTAAACTGTGGGTCAGCGAAACGTTTCGCTGa                                                            <  2:870839/149‑1 (MQ=255)
                                                                                          aCGCCTCCTTTCTTCATAAGGGGGCGTTTTTGTTTTCATGGTTAATCACCATGTAAAACGTTTCGAGGTTGATCACATTTCCGTAACGTCACGATGGTTTTCCCAACTCAGTCAGGATTAAACTGTGGGTCAGCGAAACGTTTCGCTGa                                                            >  2:1728559/1‑149 (MQ=255)
                                                                                           cGCCTCCTTTCTTCATAAGGGGGCGTTTTTGTTTTCATGGTTAATCACCATGTAAAAAGTTTCGAGGTTTATAACATTTCCGTAACGTCACGATGGTTTTCCCAAAAAAGTCAGGATTAAAATGTGGGTCCGCCGAACCGTTAGCTGAt                                                           >  1:3923468/1‑149 (MQ=255)
                                                                                            gCCTCCTTTCTTCATAAGGGGGCGTTTTTGTTTTCATGGTTAATCACCATGTAAAACGTTTCGAGGTTGATCACATTTCCGTAACGTCACGATGGTTTTCCCAACTCAGTCAGGATTAAACTGTGGGTCAGCGAAACGTTTCGCTGATg                                                          <  1:5242981/149‑1 (MQ=255)
                                                                                              ctcccTTCTTCATAAGGGGGCGAATTTGTTTTCATGGTTAATCACCATGTAAAACGTTTCGAGGTTGATCACATTTCCGTAACGTCACGATGGTTTTCCCAACTCAGTCAGGATTAAACTGTGGGTCAGCGAAACGTTTCGCTGATGga                                                        <  2:3488422/149‑1 (MQ=255)
                                                                                                ccgttcttcATACGGGGGCGTTTTTGTTTTCATGGTTAATCACCATGTAAAACGTTTCGAGGTTGATCACATTTCCGTAACGTCACGATGGTTTTCCCAACTCAGTCAGGATTAAACTGTGGGTCAGCGAAACGTTTCGCTGATGgaga                                                      <  2:2789480/146‑1 (MQ=255)
                                                                                                 ctTTCTTCATACGGGGGCGTTTTTGTTTTCATGGTTAATCACCATGTAAAACGTTTCGAGGTTGATCACATTTCCGTAACGTCACGATGGTTTTCCCAACTCAGTCAGGATTAAACTGTGGGTCAGCGAAACGTTTCGCTGATGGAGaa                                                     <  1:2300067/149‑1 (MQ=255)
                                                                                                   ttcttcATAAGGGGGCGTTTTTTTTTTCATGGTTAATCACCATGTAAAACGTTTCGAGGTTTATCACATTTCCGTAACGTCACGATGGTTTTCCCAACTCATTCAGGATTAAACTGTGGGTAAGCGAAACGTTTAGCTGATGGAGaaaa                                                   >  2:3310047/1‑149 (MQ=255)
                                                                                                   ttcttcATAAGGGGGCGTTTTTGTTTTCATGGTTAATCACCATGTAAAACGTTTCGAGGTTGATCACATTTCCGTAACGTCACGATGGTTTTCCCAACTCAGTCAGGATTAAACTGTGGGTCAGCGAAACGTTTCGCTGATGGAGaaaa                                                   <  1:820189/149‑1 (MQ=255)
                                                                                                   ttcttcATAAGGGGGCGTTTTTGTTTTCATGGTTAATCACCAAGTAAAACGTTTCGAGGTTGATCACATTTCCGTAACGTCACGATGGTTTTCCCAACTCAGTCAGGATTAAACTGTGGGTCAGCGCAACGTTTAGCTTATGGAGaaaa                                                   >  2:2183833/1‑149 (MQ=255)
                                                                                                      ttcATAAGGGGGCGTTTTTTTTTTCATGGTTAATAACCATGTAAAAAGTTTAGAGGTTGATCACAATTCCGTAACGTCACGATGGTTTTACCAACACAGTCAGGATTAAACGTTGGGTCAGCGCAACGTTTAGCGGATGGAGgaaaaaa                                                >  1:1680964/1‑149 (MQ=255)
                                                                                                      ttcATAAGGGGGCGTTTTTTTTTACATGGTAAAACACCATTAAAAACGTTACGAGGTTGATAACATTTCCGGAACGTCACGATGGTTTTCCCAACTCAGTAAGTATTAAAATGTGGGACCGCGAAACGTTTCGCTGATGGAGaaaaaaa                                                >  2:2101043/1‑149 (MQ=255)
                                                                                                        cATAAGGGGGCGTTTTTGTTTTCATGGTTAATCACCATGTAAAACGTTTCGAGGTTGATCACATTTCCGTAACGTCACGATGGTTTTCCCAACTCAGTCAGGATTAAACTGTGGGTCAGCGAAACGTTTCGCTGATGGAGAAAAAAATg                                              <  2:4316324/149‑1 (MQ=255)
                                                                                                           aaGGGGGCGTTTTTGTTTTCATGGTTAATCACCATGTAAAACGTTTCGAGGTTGATCACATTTCCGTAACGTCACGATGGTTTTCCCAACTCAGTCAGGATTAAACTGTGGGTCAGCGAAACGTTTCGCTGATGGAGAAAAATATGaaa                                           <  2:4799056/149‑1 (MQ=255)
                                                                                                              ggggCGTTTTTGTTTTCATGGTTAATCACCATGTAAAACGTTTCGAGGTTGATCACATTTCCGTAACGTCACGATGGTTTTCCCAACTCAGTCAGGATTAAACTGTGGGTCAGCGAAACGTTTCGCTGATGGAGAAAAAAATGaaaaaa                                        <  2:3956219/149‑1 (MQ=255)
                                                                                                                 gCGTTTTTGTTTTCATGGTTAATCACCATGTAAAACGTTTCGAGGTTGATCACATTTCCGTAACGTCACGATGGTTTTCCCAACTCAGTCAGGATTAAACTGTGGGTCAGCGAAACGTTTCGCTGATGGAGAAAAAAATGAAAAAAGGc                                     <  2:2669492/149‑1 (MQ=255)
                                                                                                                    tttttGTTTTCATGGTTAATCACCATGTAAAACGTTTCGAGGTTGATCACATTTCCGTAACGTCACGATGGTTTTCCCAACTCAGTCAGGATTAAACTGTGGGTCAGCGAAACGTTTCGCTGATGGAGAAAAAAATGAAAAAAGGCAcc                                  >  2:4308078/1‑149 (MQ=255)
                                                                                                                       ttGTTTTCATGGTTAATCACCATGTAAAACGTTTCGAGGTTGATCACATTTCCGTAACGTCACGATGGTTTTCCCAACTCAGTCAGGATTAAACTGTGGGTCAGCGAAACGTTTCGCTGATGGAGAAAAAAATGAAAAAAGGCACCGtt                               <  1:488785/149‑1 (MQ=255)
                                                                                                                        tGTTTTCATGGTTAATCACCATGTAAAACGTTTCGAGGTTGATCACATTTCCGTAACGTCACGATGGTTTTCCCAACTCAGTCAGGATTAAACTGTGGGTCAGCGAAACGTTTCGCTGATGGAGAAAAAAATGAAAAAAGGCACCGTTc                              <  1:3370781/149‑1 (MQ=255)
                                                                                                                        tGTTTTCATGGTTAATCACCATGTAAAACGTTTCGAGGTTGATCACATTTCCGTAACGTCACGATGGTTTTCCCAACTCAGTCAGGATTAAACTGTGGGTCAGCGAAACGTTTCGCTGATGGAGAAAAAAATGAAAAAAGGCACCGTTc                              <  2:1698464/149‑1 (MQ=255)
                                                                                                                        tGTTTTCATGGTTAATCACCATGTAAAACGTTTCGAGGTTGATCACATTTCCGTAACGTCACGATGGTTTTCCCAACTCAGTCAGGATTAAACTGTGGGTCAGCGAAACGTTTCGCGGTTGGAGTAAAAAATTAAAAAAAGCCCCCTTc                              >  1:3115704/1‑149 (MQ=255)
                                                                                                                         gTTTTCATGGTTAATCACCATGTAAAACGTTTCGAGGTTGATCACATTTCCGTAACGTCACGATGGTTTTCCCAACTCAGTCAGGATTAAACTGTGGGTCAGCGAAACGTTTCGCTGATGGAGAAAAAAATGAAAAAAGGCACCGTTCt                             >  2:253746/1‑149 (MQ=255)
                                                                                                                          ttttCATGGTTAATCACCATGTAAAACGTTTCGAGGTTGATCACATTTCCGTAACGTCACGATGGTTTTCCCAACTCAGTCAGGATTAAACTGTGGGTCAGCGAAACGTTTCGCTGATGGAGAAAAAAATGAAAAAAGGCACCGTTCtt                            <  2:2653259/149‑1 (MQ=255)
                                                                                                                               aTGGTTAATCACCATGTAAAACGTTTCGAGGTTGATCACATTTCCGTAACGTCACGATGGTTTTCCCAACTCAGACAGGATTAAACTGTGGGTCAGCGAAACGTTTCGCTGATGGAGAAAAAAATGAAAAAAGGCACCGTTCTTAATTc                       <  2:1991281/149‑1 (MQ=255)
                                                                                                                                tGGTTAATCACCATGTAAAACGTTTCGAGGTTGATCACATTTCCGTAACGTCACGATGGTTTTCCCAACTCAGTCAGGATTAAACTGTGGGTCAGCGGCACGGGTCGCTGATGGGGAAAAAAATGAAAAAAAGAACCCGGCTTAATTCt                      >  1:549733/1‑149 (MQ=255)
                                                                                                                                  gTTAATCACCATGTAAAACGTTTCGAGGTTGATCACATTTCCGTAACGTCACGATGGTTTTCCCAACTCAGTCAGGATTAAACTGTGGGTCAGCGAAACGTTTCGCTGATGGAGAAAAAAATGAAAAAAGGCACCGTTCTTAATTCTGa                    <  1:2909208/149‑1 (MQ=255)
                                                                                                                                  gTTAATCACCATGTAAAACGTTTCGAGGTTGATCACATTTCCGTAACGTCACGATGGTTTTCCCAACTCAGTCAGGATTAAACTGTGGGTCAGCGAAACGTTTCGCTGATGGAGAAAAAAATGAAAAAAGGCACCGTTCTTAATTCTGa                    >  2:3694808/1‑149 (MQ=255)
                                                                                                                                     aaTCACCATGTAAAACGTTTCGAGGTTGATCACATTTCCGTAACGTCACGATGGTTTTCCCAACTCAGTCAGGATTAAACTGTGGGTCAGCGAAACGTTTCGCTGATGGAGAAAAAAATGAAAAAAGGCACCGTTCTTAATTCTGatat                 <  1:1454882/149‑1 (MQ=255)
                                                                                                                                      aTCACCATGTAAAACGTTTCGAGGTTGATCACATTTCCGTAACGTCACGATGGTTTTCCCAACTCAGTCAGGATTAAACTGTGGGTCAGCGAAACGTTTCGCTGATGGAGAAAAAAATGAAAAAAGGCACCGTTCTTAATTCTGATAtt                >  2:1347412/1‑149 (MQ=255)
                                                                                                                                      aTCACCATGTAAAACGTTTCGAGGTTGATCACATTTCCGTAACGTCACGATGGTTTTCCCAACTCAGTCAGGATTAAACTGTGGGTCAGCGAAACGTTTCGCTGATGGAGAAAAAAATGAAAAAAGGCACCGTTCTTAATTCTGATAtt                >  2:4652829/1‑149 (MQ=255)
                                                                                                                                      aTCACCATGTAAAACGTTTCGAGGTTGATCACATTTCCGTAACGTCACGATGGTTTTCCCAACTCAGTCAGGATTAAACTGTGGGTCAGCGAAACGTTTCGCTGATGGAGAAAAAAATGAAAAAAGGCACCGTTCTTAATTCTGATAtt                >  2:3051581/1‑149 (MQ=255)
                                                                                                                                        cACCATGTAAAACGTTTCGAGGTTGATCACATTTCCGTAACGTCACGATGGTTTTCCCAACTCAGTCAGGATTAAACTGTGGGTCAGCGAAACGTTTCGCTGATGGAGAAAAAAATGAAAAAAGGCACCGTTCTTAATTCTGATATTTc              <  2:4927521/149‑1 (MQ=255)
                                                                                                                                          ccATGTAAAACGTTTCGAGGTTGATCACATTTCCGTAACGTCACGATGGTTTTCCCAACTCAGTCAGGATTAAACTGTGGGTCAGCGGAACGGTTCGCGGGTGGAGCAAAAAATGAAAAAAGGCACCGGTCTTAATTCTTAtaattaat            >  1:4525220/1‑149 (MQ=255)
                                                                                                                                          ccATGTAAAACGTTTCGAGGTTGATCACATTTCCGTAACGTCACGATGGTTTTCCCAACTCAGTCAGGATTAAACTGTGGGTCAGCGAAACGTTTCGCGGATTGAGAAAAAAAATAAAAAATGCACCGTTCGTAATTATGATATTTAAt            >  1:5223803/1‑149 (MQ=255)
                                                                                                                                             tGTAAAACGTTTCGAGGTTGATCACATTTCCGTAACGTCACGATGGTTTTCCCAACTCAGTCCGGATTAAACTGTGGGTCAGCGACACGTTTCGCTGATGGAGAAAAAAATGAAAAAAGGCACCGTTCTTAATTCTGATATTTCATCgg         >  2:2985092/1‑149 (MQ=255)
                                                                                                                                               tAAAACGTTTCGAGGTTGATCACATTTCCGTAACGTCACGATGGTTTTCCCAACTCAGTCAGGATTAAACTGTGGGTCAGCGAAACGTTTCGCTGATGGAGAAAAAAATGAAAAAAGGCACCGTTCTTAATTCTGATATTTCATCGGTg       <  1:3972816/149‑1 (MQ=255)
                                                                                                                                                    cGTTTCGAGGTTGATCACATTTCCGTAACGTCACGATGGTTTTCCCAACTCAGTCAGGATTAAACTGTGGGTCAGCGAAACGTTTCGCTGATGGAGAAAAAAATTAAAAAAGGCACCGTTCTTAATTCTGATATTTCATCGGTGAtatc  >  1:4350955/1‑149 (MQ=255)
                                                                                                                                                    cGTTTCGAGGTTGATCACATTTCCGTAACGTCACGATGGTTTTCCCAACTCAGTCAGGATTAAACTGTGGGTCAGCGAAACGTTTCGCTGATGGAGAAAAAAATGAAAAAAGGCACCGTTCTTAATTCTGATATTTCATCGGTGAtctc  <  2:536624/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
CAACGTAATGCGCTGCGTGCACCAGATCAATTTGAAATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGTTTTTGTTTTCATGGTTAATCACCATGTAAAACGTTTCGAGGTTGATCACATTTCCGTAACGTCACGATGGTTTTCCCAACTCAGTCAGGATTAAACTGTGGGTCAGCGAAACGTTTCGCTGATGGAGAAAAAAATGAAAAAAGGCACCGTTCTTAATTCTGATATTTCATCGGTGATCTC  >  CP009273/3926461‑3926757

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑