Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,062,424 G→T 85.2% I71I (ATC→ATA)  yihQ ← alpha‑glucosidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,062,4240GT85.2% 896.3 / 163.3 339I71I (ATC→ATA) yihQalpha‑glucosidase
Reads supporting (aligned to +/- strand):  ref base G (27/23);  new base T (115/174);  total (142/197)
Fisher's exact test for biased strand distribution p-value = 6.43e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.33e-01

Only 100 of 339 total aligned reads displayed.

GCAGCCAGATACGGTTGTGGTTAAGGTTGTCGTTTTGTAGTTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACGATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGATTATCTTTGCTATGGGTTAAAATAAGACGTTGTTGAAAAT  >  CP009273/4062279‑4062569
                                                                                                                                                 |                                                                                                                                                 
gcAGCCAGATACGGTTGTGGTTAAGGTTGTCGTTTTGTAGTTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATg                                                                                                                                                <  1:2075751/149‑1 (MQ=255)
  aGCCAGATACGGTTGTGGTTAAGGTTGTCGTTTTGTAGTTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGCCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGc                                                                                                                                              >  1:2774582/1‑149 (MQ=255)
   gCCAGATAGGGTTGTGGTTATGGTTGTCGTTTTGTAGTTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCg                                                                                                                                             <  1:36144/149‑1 (MQ=255)
    ccAGATACGGTTGTGGTTAAGGTTGTCGTTTTGTAGTTCCAGCAATACACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGt                                                                                                                                            <  2:3943149/149‑1 (MQ=255)
       gATACGGTTGTGGTTAAGGTTGTCGTTTTGTAGTTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCgg                                                                                                                                         >  1:3734/1‑149 (MQ=255)
       gATACGGTTGTGGTTAAGGTTGTCGTTTTGTAGTTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCgg                                                                                                                                         <  2:1912649/149‑1 (MQ=255)
        aTACGGTTGTGGTTAAGGTTGTCGTTTTGTAGTTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGt                                                                                                                                        <  2:571148/149‑1 (MQ=255)
          aCGGTAGTGGTTAAGGTTGTCGTTTTGTAGTTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACTGCTGAAATGAATTAACCAACCATCCGGAGACTGGCTGACTATGGCGTCGGTaa                                                                                                                                      >  1:3930211/1‑149 (MQ=255)
            ggTTGTGGTTAAGGTTGTCGTTTTGTAGTTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAgc                                                                                                                                    <  2:2535048/149‑1 (MQ=255)
               tgtgGTTAAGGTTGTCGTTTTGTAGTTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCa                                                                                                                                 <  2:2950774/149‑1 (MQ=255)
                gtgGTTAAGGTTGTCGTTTTGTAGTTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCaa                                                                                                                                >  2:1857047/1‑149 (MQ=255)
                 tgGTTAAGGTTGTCGTTTTGTAGTTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAAt                                                                                                                               <  1:3356446/149‑1 (MQ=255)
                  ggTTAAGGTTGTCGTTTTGTAGTTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAAtt                                                                                                                              <  1:2864841/149‑1 (MQ=255)
                       aGGTTGTCGTTTTGTAGTTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTtctc                                                                                                                         >  2:811622/1‑149 (MQ=255)
                       aGGTTGTCGTTTTGTAGTTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACGATGGCGTCGGTAAGCGCAATTTtctc                                                                                                                         >  1:3363249/1‑149 (MQ=255)
                          ttGTCGTTTTGTAGTTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTg                                                                                                                      >  2:4324406/1‑149 (MQ=255)
                            gTCGTTTTGTAGTTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGGCGGTAAGCGCAATTTTCTCCTGTa                                                                                                                    >  1:3221519/1‑149 (MQ=255)
                               gTTTTGTAGTTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATACGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAgat                                                                                                                 >  2:3443031/1‑147 (MQ=255)
                                ttttGTAGTTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGttt                                                                                                                <  1:3790492/149‑1 (MQ=255)
                                ttttGTAGTTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGttt                                                                                                                >  1:3429437/1‑149 (MQ=255)
                                  ttGTAGTTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTAt                                                                                                              >  2:629022/1‑149 (MQ=255)
                                    gTAGTTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCt                                                                                                            <  2:1537523/149‑1 (MQ=255)
                                     tAGTTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCtt                                                                                                           >  1:431268/1‑149 (MQ=255)
                                      aGTTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCttt                                                                                                          <  2:4251817/149‑1 (MQ=255)
                                      aGTTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACGATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCttt                                                                                                          <  2:1003014/149‑1 (MQ=255)
                                        ttCCAGCAATACACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTaa                                                                                                        <  1:4451573/149‑1 (MQ=255)
                                         tCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAAt                                                                                                       >  2:3130901/1‑149 (MQ=255)
                                         tCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAAt                                                                                                       <  1:2699819/149‑1 (MQ=255)
                                         tCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACGATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAAt                                                                                                       <  2:329771/149‑1 (MQ=255)
                                            aGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCt                                                                                                    >  1:1998403/1‑149 (MQ=255)
                                            aGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACGATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCt                                                                                                    <  2:75498/149‑1 (MQ=255)
                                               aaTAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGaa                                                                                                 >  2:4195390/1‑149 (MQ=255)
                                                aTAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGaaa                                                                                                >  1:2629127/1‑149 (MQ=255)
                                                 tAAACGCCCCTGATCGTCGGCAGAGATAGTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAAt                                                                                               <  2:3225174/149‑1 (MQ=255)
                                                     cGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACTACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTAcc                                                                                           <  2:1448777/149‑1 (MQ=255)
                                                      gCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACcg                                                                                          <  2:4569271/149‑1 (MQ=255)
                                                         ccTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCgg                                                                                       >  2:1316127/1‑149 (MQ=255)
                                                         ccTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACGATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCgg                                                                                       >  2:1724676/1‑149 (MQ=255)
                                                           tGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGaa                                                                                     <  2:2434720/149‑1 (MQ=255)
                                                           tGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGaa                                                                                     <  2:1088740/149‑1 (MQ=255)
                                                           tGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGaa                                                                                     <  1:5206807/149‑1 (MQ=255)
                                                             aTCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACa                                                                                   <  1:4875609/149‑1 (MQ=255)
                                                              tcgtcgGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACat                                                                                  <  2:4446795/149‑1 (MQ=255)
                                                                gtcgGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACatat                                                                                <  1:4734716/149‑1 (MQ=255)
                                                                gtcgGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACatat                                                                                <  1:1897940/149‑1 (MQ=255)
                                                                   ggCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGa                                                                             <  1:3045600/149‑1 (MQ=255)
                                                                    gCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGACCATATCGat                                                                            >  2:1084633/1‑149 (MQ=255)
                                                                    gCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGat                                                                            >  2:1908361/1‑149 (MQ=255)
                                                                    gCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGat                                                                            >  1:4195280/1‑149 (MQ=255)
                                                                     cAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGata                                                                           <  1:5009619/149‑1 (MQ=255)
                                                                     cAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGata                                                                           <  2:1232281/149‑1 (MQ=255)
                                                                       gagaTATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATc                                                                         >  1:2523678/1‑149 (MQ=255)
                                                                       gagaTATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACGATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATc                                                                         >  1:4529303/1‑149 (MQ=255)
                                                                        agaTATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATcc                                                                        >  1:2397928/1‑149 (MQ=255)
                                                                           tatTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCt                                                                     >  1:1017701/1‑149 (MQ=255)
                                                                            cttCAGCGTGGCGCTACTGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACGGGCTGACGATGGCGTCGGTAAGCGCAATTGTCTCCTGTAGTTTATCTTTACTGCTGAAATTACCGCGGAACATATCGATATCCGCta                                                                    <  2:3774399/148‑1 (MQ=255)
                                                                            atTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCta                                                                    >  1:4651866/1‑149 (MQ=255)
                                                                            atTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCta                                                                    >  2:2190323/1‑149 (MQ=255)
                                                                            atTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACGATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCta                                                                    <  2:1156423/149‑1 (MQ=255)
                                                                             ttCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCtat                                                                   >  2:5098684/1‑149 (MQ=255)
                                                                                  cGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTg                                                              <  2:348971/149‑1 (MQ=255)
                                                                                        gcTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCaa                                                        >  2:5041501/1‑149 (MQ=255)
                                                                                        gcTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCaa                                                        >  1:4889680/1‑149 (MQ=255)
                                                                                        gcTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACGATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCaa                                                        <  2:4887528/149‑1 (MQ=255)
                                                                                           aaTGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATcc                                                     >  1:2559403/1‑149 (MQ=255)
                                                                                                 aGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAAc                                               <  1:59966/149‑1 (MQ=255)
                                                                                                      cACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGa                                          >  2:2314238/1‑149 (MQ=255)
                                                                                                       aCGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGAt                                         <  2:4063504/149‑1 (MQ=255)
                                                                                                         ggCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGATTa                                       <  1:1662556/149‑1 (MQ=255)
                                                                                                         ggCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACGATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGATTa                                       >  2:3569365/1‑149 (MQ=255)
                                                                                                            tGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGATTATCt                                    <  1:4256697/149‑1 (MQ=255)
                                                                                                            tGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGATTATCt                                    <  2:3428487/149‑1 (MQ=255)
                                                                                                             gAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGATTATCtt                                   <  2:2034398/149‑1 (MQ=255)
                                                                                                             gAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGATTATCtt                                   <  1:149294/149‑1 (MQ=255)
                                                                                                             gAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGATTATCtt                                   <  1:2314484/149‑1 (MQ=255)
                                                                                                               aaTGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGATTATCTTTg                                 <  2:466617/149‑1 (MQ=255)
                                                                                                               aaTGAATTAACCAACCATCCGGTGACTGGCTGACGATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGATTATCTTTg                                 >  1:654521/1‑149 (MQ=255)
                                                                                                                   aaTTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGATTATCTTTGCTAt                             >  1:4785641/1‑149 (MQ=255)
                                                                                                                    aTTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGATTATCTTTGCTATg                            >  2:4986193/1‑149 (MQ=255)
                                                                                                                    aTTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGATTATCTTTGCTATg                            >  1:4435243/1‑149 (MQ=255)
                                                                                                                     ttAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGATTATCTTTGCTATgg                           <  2:2668588/149‑1 (MQ=255)
                                                                                                                       aaccaaccATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATGCATAAACAAGGATTATCTTTGCTATGGGt                         <  2:2952622/149‑1 (MQ=255)
                                                                                                                         ccaaccaTCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGATTATCTTTGCTATGGGTTa                       >  2:5166207/1‑149 (MQ=255)
                                                                                                                         ccaaccaTCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGATTATCTTTGCTATGGGTTa                       >  1:2941016/1‑149 (MQ=255)
                                                                                                                         ccaaccaTCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATAACGATATCCGCTATACCTGAGCCAATCCATAAACAAGGATTATCTTTGCTATGGGGTa                       >  1:1777605/1‑149 (MQ=255)
                                                                                                                          caaccaTCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGATTATCTTTGCTATGGGTTaa                      >  2:3628401/1‑149 (MQ=255)
                                                                                                                          caaccaTCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGATTATCTTTGCTATGGGTTaa                      >  1:5201740/1‑149 (MQ=255)
                                                                                                                            accaTCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGATTATCTTTGCTATGGGTTaaaa                    <  1:506469/149‑1 (MQ=255)
                                                                                                                             ccaTCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGATTATCTTTGCTATGGGTTAAAAt                   <  1:2190323/149‑1 (MQ=255)
                                                                                                                             ccaTCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGATTATCTTTGCTATGGGTTAAAAt                   <  1:4805394/149‑1 (MQ=255)
                                                                                                                               aTCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGATTATCTTTGCTATGGGTTAAAATaa                 <  1:934277/149‑1 (MQ=255)
                                                                                                                               aTCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGATTATCTTTGCTATGGGTTAAAATaa                 <  1:3555349/149‑1 (MQ=255)
                                                                                                                               aTCCGGTGACTGGCTGACGATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGATTATCTTTGCTATGGGTTAAAATaa                 <  1:3408596/149‑1 (MQ=255)
                                                                                                                                tCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGATTATCTTTGCTATGGGTTAAAATAAg                >  2:1581211/1‑149 (MQ=255)
                                                                                                                                tCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGATATACCTGAGCCAATCCATAAACAATGATTATCTTTGATATGGGTTAAAATAAg                >  1:2262253/1‑149 (MQ=255)
                                                                                                                                 ccGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGATTATCTTTGCTATGGGTTAAAATAAGa               <  1:1261112/149‑1 (MQ=255)
                                                                                                                                      gACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGATTATCTTTGCTATGGGTTAAAATAAGACgttg          <  2:3930377/149‑1 (MQ=255)
                                                                                                                                      gACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGATTATCTTTGCTATGGGTTAAAATAAGACgttg          >  1:4117107/1‑149 (MQ=255)
                                                                                                                                         tGGCTGACTCTGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGATTATCTTTGCTATGGGTTAAAATAAGACGttgttg       <  2:2774582/149‑1 (MQ=255)
                                                                                                                                          ggCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGATTATCTTTGCTATGGGTTAAAATAAGACGTTGTTGa      <  2:4306206/149‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                 
GCAGCCAGATACGGTTGTGGTTAAGGTTGTCGTTTTGTAGTTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACGATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGATTATCTTTGCTATGGGTTAAAATAAGACGTTGTTGAAAAT  >  CP009273/4062279‑4062569

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑