BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	4,211,576	G→T	5.6%	S280R (AGC→AGA)	arpA ←	ankyrin repeat protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	4,211,576	0	G	T	5.6%	1179.6 / 31.5	308	S280R (AGC→AGA)	arpA	ankyrin repeat protein
Reads supporting (aligned to +/- strand): ref base G (176/114); new base T (9/9); total (185/123)
Fisher's exact test for biased strand distribution p-value = 4.58e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 312 total aligned reads displayed.

TTTTAACATCAATAGCTGTTCTTTCGGAAGTGCTGGAATTATTGCAACAGATTGATTAATAACATGATCATTTGACCATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCGCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCAGTTGTGTTCTTTATTCTTAGCCGGACCGTCAAAGCATGGTTATCCACCAGTAATATGGCTGCCATAACACTGATGC  >  CP009273/4211429‑4211724

  ttAACATCAATAGCTGTTCTTTCGGAAGTGCTGGAATTATTGCAACAGATTGATTAATAACATGATCATTTGACCATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCGCTc                                                                                                                                                   <  2:2734367/149‑1 (MQ=255)

  ttAACATCAATAGCTGTTCTTTCGGAAGTGCTGGAATTATTGCAACAGATTGATTAATAACATGATCATTTGACCATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCGCTc                                                                                                                                                   <  2:108297/149‑1 (MQ=255)

    aaCATCAATAGCTGTTCTTTCGGAAGTGCTGGAATTATTGCAACAGATTGATTAATAACATGATCATTTGACCATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCGCTCAt                                                                                                                                                 >  1:1558026/1‑149 (MQ=255)

    aaCATCAATAGCTGTTCTTTCGGAAGTGCTGGAATTATTGCAACAGATTGATTAATAACATGATCATTTGACCATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCGCTCAt                                                                                                                                                 >  1:2533626/1‑149 (MQ=255)

     aCATCAATAGCTGTTCTTTCGGAAGTGCTGGAATTATTGCAACAGATTGATTAATAACATGATCATTTGACCATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCGCTCATa                                                                                                                                                >  2:757085/1‑149 (MQ=255)

     aCATCAATAGCTGTTCTTTCGGAAGTGCTGGAATTATTGCAACAGATTGATTAATAACATGATCATTTGACCATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCGCTCATa                                                                                                                                                >  2:4084905/1‑149 (MQ=255)

     aCATCAATAGCTGTTCTTTCGGAAGTGCTGGAATTATTGCAACAGATTGATTAATAACATGATCATTTGACCATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCGCTCATa                                                                                                                                                >  1:2840966/1‑149 (MQ=255)

      cATCAATAGCTGTTCTTTCGGAAGTGCTGGAATTATAGCAACAGATTGATTAATAACATGATCATTTGACCATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCGCTCATaa                                                                                                                                               <  2:2945420/149‑1 (MQ=255)

          aaTAGCTGTTCTTTCGGAAGTGCTGGAATTATTGCAACAGATTGATTAATAACATGATCATTTGACCATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCGCTCATAATTCt                                                                                                                                           <  1:743126/149‑1 (MQ=255)

          aaTAGCTGTTCTTTCGGAAGTGCTGGAATTATTGCAACAGATTGATTAATAACATGATCATTTGACCATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCGCTCATAATTCt                                                                                                                                           >  1:1040522/1‑149 (MQ=255)

               cTGTTCTTTCGGAAGTGCTGGAATTATTGCAACAGATTGATTAATAACATGATCATTTGACCATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCGCTCATAATTCTTAttt                                                                                                                                      >  2:4293939/1‑149 (MQ=255)

               cTGTTCTTTCGGAAGTGCTGGAATTATTGCAACAGATTGATTAATAACATGATCATTTGACCATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCGCTCATAATTCTTAttt                                                                                                                                      <  2:4277677/149‑1 (MQ=255)

                 gTTCTTTCGGAAGTGCTGGAATTATTGCAACAGATTGATTAATAACATGATCATTTGACCATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCGCTCATAATTCTTATTTTa                                                                                                                                    >  2:2596074/1‑149 (MQ=255)

                 gTTCTTTCGGAAGTGCTGGAATTATTGCAACAGATTGATTAATAACATGATCATTTGACCATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCGCTCATAATTCTTATTTTa                                                                                                                                    >  1:3584758/1‑149 (MQ=255)

                        cGGAAGTGCTGGAATTAGTGCAACAGATTGATTAATAACATGATCATTTGACCATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCGCTCATAATTCTTATTTTATCgttag                                                                                                                             >  2:132230/1‑149 (MQ=255)

                           aaGTGCTGGAATTATTGCAACAGATTGATTAATAACATGATCATTTGACCATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCTCTCATAATTCTTATTTTATCgttagtta                                                                                                                          <  1:1370139/149‑1 (MQ=255)

                                 tGGAATTATTGCAACAGATTGATTAATAACATGATCATTTGACCATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCGCTCATAATTCTTATTTTATCGTTAGTTACATTAg                                                                                                                    <  1:4512573/149‑1 (MQ=255)

                                     attattGCAACAGATTGATTAATAACATGATCATTTGACCATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCGCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATc                                                                                                                >  1:4386624/1‑149 (MQ=255)

                                      ttattGCAACAGATTGATTAATAACATGATCATTTGACCATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCGCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCa                                                                                                               <  2:1500352/149‑1 (MQ=255)

                                      ttattGCAACAGATTGATTAATAACATGATCATTTGACCATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCGCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCa                                                                                                               <  2:4031920/149‑1 (MQ=255)

                                         ttGCAACAGATTGATTAATAACATGATCATTTGACCATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCGCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATaa                                                                                                            <  1:4454819/149‑1 (MQ=255)

                                         ttGCAACAGATTGATTAATAACATGATCATTTGACCATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCGCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATaa                                                                                                            <  1:3905091/149‑1 (MQ=255)

                                           gCAACAGATTGATTAATAACATGATCATTTGACCATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCGCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAAc                                                                                                          <  1:1852662/149‑1 (MQ=255)

                                            cAACAGATTGATTAATAACATGATCATTTGACCATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCGCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAAcc                                                                                                         >  2:328317/1‑149 (MQ=255)

                                              aCAGATTGATTAATAACATGATCATTTGACCATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCGCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGa                                                                                                       >  1:1159137/1‑149 (MQ=255)

                                                 gattgattAATAACATGATCATTTGACCATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCGCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAAc                                                                                                    >  1:2091992/1‑149 (MQ=255)

                                                   ttgattAATAACATGATCATTTGACCATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCGCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCa                                                                                                  >  2:3980958/1‑149 (MQ=255)

                                                          ataaCATGATCATTTGACCATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCGCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATg                                                                                           >  1:3476690/1‑149 (MQ=255)

                                                             aCATGATCATTTGACCATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCGCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGt                                                                                        >  2:3663607/1‑149 (MQ=255)

                                                             aCATGATCATTTGACCATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCGCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGt                                                                                        >  2:4964329/1‑149 (MQ=255)

                                                                 gATCATTTGACCATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCGCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACAt                                                                                    >  1:1044875/1‑149 (MQ=255)

                                                                   tCATTTGACCATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCGCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATcc                                                                                  >  2:5149681/1‑149 (MQ=255)

                                                                   tCATATGACCATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCGCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATcc                                                                                  <  2:1646313/149‑1 (MQ=255)

                                                                     aTTTGACCATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCGCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCtt                                                                                <  1:525188/149‑1 (MQ=255)

                                                                     aTTTGACCATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCGCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCtt                                                                                >  2:3049257/1‑149 (MQ=255)

                                                                     aTTTGACCATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCGCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCtt                                                                                <  1:4792350/149‑1 (MQ=255)

                                                                       ttGACCATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCGCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCa                                                                              >  1:1009812/1‑149 (MQ=255)

                                                                          aCCATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCGCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCAGtt                                                                           <  2:4741439/149‑1 (MQ=255)

                                                                           ccATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCGCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCAGTtg                                                                          <  1:3761327/149‑1 (MQ=255)

                                                                              tttCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCGCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCAGTTgtgt                                                                       <  1:2746689/149‑1 (MQ=255)

                                                                               ttCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCGCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCAGTTGTGtt                                                                      <  1:3182152/149‑1 (MQ=255)

                                                                               ttCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCGCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCAGTTGTGtt                                                                      <  1:332611/149‑1 (MQ=255)

                                                                                   ggaggCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCGCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCAGTTGTGTTCttt                                                                  >  2:4019029/1‑149 (MQ=255)

                                                                                     aggCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCGCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCAGTTGTGTTCTTTAt                                                                >  2:2392607/1‑149 (MQ=255)

                                                                                       gCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCGCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCAGTTGTGTTCTTTATTc                                                              <  2:2967185/149‑1 (MQ=255)

                                                                                         tataATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATCTTCTCTTTGCTGTCGCTCATAATTCTTCTTTTATGGTTAGTTACATTAGGATCATAAACCGACACCACGGAGTGGGTCCATCGTGCAGTTGTGGTCTTTATTCtt                                                            >  1:884910/1‑149 (MQ=255)

                                                                                         tataATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCGCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCAGTTGTGTTCTTTATTCtt                                                            >  1:3407810/1‑149 (MQ=255)

                                                                                          ataATCTACATTCATAAAATCCATCAGAGGATAGTGTTTAATATTCTCTTTGCTTTCGCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCAGTTGTGTTCTTTATTCTTa                                                           >  1:489028/1‑149 (MQ=255)

                                                                                            aaTCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCGCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCAGTTGTGTTCTTTATTCTTAGc                                                         <  1:5118752/149‑1 (MQ=255)

                                                                                             aTCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCGCTCATAATTCTTATTTTATCGTTAGTTACATTCGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCAGTTGTGTTCTTTATTCTTAGcc                                                        >  2:2075100/1‑149 (MQ=255)

                                                                                             aTCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCGCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCAGTTGTGTTCTTTATTCTTAGcc                                                        >  2:2193726/1‑149 (MQ=255)

                                                                                              tCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCGCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCAGTTGTGTTCTTTATTCTTAGCCg                                                       <  1:1942805/149‑1 (MQ=255)

                                                                                              tCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCGCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACAACGTAATGGGTACATCCTTCAGTTGTGTTCTTTATTCTTAGCCg                                                       >  2:3510145/1‑149 (MQ=255)

                                                                                               cTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCGCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGTGTACATCCTTAAGTTGTTTTCTTTATTATTTGCCgg                                                      >  2:2974726/1‑149 (MQ=255)

                                                                                                tACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCGCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCAGTTGTGTTCTTTATTCTTAGCCGGa                                                     <  2:2840966/149‑1 (MQ=255)

                                                                                                       aTAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCGCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCAGTTGTGTTCTTTATTCTTAGCCGGACCGTCaa                                              <  1:4832727/149‑1 (MQ=255)

                                                                                                        tAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCGCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCAGTTGTGTTCTTTATTCTTAGCCGGACCGTCaaa                                             >  2:1640404/1‑149 (MQ=255)

                                                                                                         aaaaTCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCTCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCAGTTGTGTTCTTTATTCTTAGCCGGACCGTCAAAg                                            <  2:620098/149‑1 (MQ=255)

                                                                                                         aaaaTCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCTCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCAGTTGTGTTCTTTATTCTTAGCCGGACCGTCAAAg                                            <  2:1050121/149‑1 (MQ=255)

                                                                                                         aaaaTCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCGCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCAGTTGTGTTCTTTATTCTTAGCCGGACCGTCAAAg                                            >  1:1352763/1‑149 (MQ=255)

                                                                                                          aaaTCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCGCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCAGTTGTGTTCTTTATTCTTAGCCGGACCGTCAAAGc                                           <  1:1596178/149‑1 (MQ=255)

                                                                                                          aaaTCCATCAGAGAATAGTGTTTAATAATCTCTTTGCTTTCGCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCAGTTGTGTTCTTTATTCTTAGCCGGACCGTCAAAGc                                           >  1:2575379/1‑149 (MQ=255)

                                                                                                           aaTCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCTCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCAGTTGTGTTCTTTATTCTTAGCCGGACCGTCAAAGCa                                          <  1:1697133/149‑1 (MQ=255)

                                                                                                           aaTCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCGCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCAGTTGTGTTCTTTATTCTTAGCCGGACCGTCAAAGCa                                          <  2:144320/149‑1 (MQ=255)

                                                                                                            aTCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCGCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCAGTTGTGTTCTTTATTCTTAGCCGGACCGTCAAAGCAt                                         >  1:5056429/1‑149 (MQ=255)

                                                                                                             tCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCGCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCAGTTGTGTTCTTTATTCTTAGCCGGACCGTCAAAGCATg                                        >  2:4684628/1‑149 (MQ=255)

                                                                                                               cATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCGCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCAGTTGTGTTCTTTATTCTTAGCCGGACCGTCAAAGCATGGt                                      <  2:2253320/149‑1 (MQ=255)

                                                                                                                aTCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCGCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCAGTTGTGTTCTTTATTCTTAGCCGGACCGTCAAAGCATTGtt                                     >  2:1165222/1‑149 (MQ=255)

                                                                                                                aTCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCGCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAAAGGGTACATACTTCAGTTGTGTTCTTTATTCTTAGCCGGACCGTCAAAGCATGGtt                                     >  1:4920154/1‑149 (MQ=255)

                                                                                                                 tCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCGCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCAGTTGTGTTCTTTATTCTTAGCCGGACCGTCAAAGCATGGTTa                                    >  2:1707155/1‑149 (MQ=255)

                                                                                                                 tCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCGCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCAGTTGTGTTCTTTATTCTTAGCCGGACCGTCAAAGCATGGTTa                                    <  2:2135754/149‑1 (MQ=255)

                                                                                                                  cAGAGAATAGTGTTTAATATTCTCTTTGCTTTCGCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCAGTTGTGTTCTTTATTCTTAGCCGGACCGTCAAAGCATGGTTAt                                   <  1:2038527/149‑1 (MQ=255)

                                                                                                                  cAGAGAATAGTGTTTAATATTCTCTTTGCTTTCGCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCAGTTGTGTTCTTTATTCTTAGCCGGACCGTCAAAGCATGGTTAt                                   >  1:4368036/1‑149 (MQ=255)

                                                                                                                   agagAATAGTGTTTAATATTCTCTTTGCTTTCGCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCAGTTGTGTTCTTTATTCTTAGCCGGACCGTCAAAGCATGGTTATc                                  >  2:1221291/1‑149 (MQ=255)

                                                                                                                   agagAATAGTGTTTAATATTCTCTTTGCTTTCGCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCAGTTGTGTTCTTTATTCTTAGCCGGACCGTCAAAGCATGGTTATc                                  >  2:3523199/1‑149 (MQ=255)

                                                                                                                    gagaATAGTGTTTAATATTCTCTTTGCTTTCGCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCAGTTGTGTTCTTTATTCTTAGCCGGACCGTCAAAGCATGGTTATcc                                 >  1:2955234/1‑149 (MQ=255)

                                                                                                                    gagaATAGTGTTTAATATTCTCTTTGCTTTCGCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCAGTTGTGTTCTTTATTCTTAGCCGGACCGTCAAAGCATGGTTATcc                                 >  1:330517/1‑149 (MQ=255)

                                                                                                                      gaATAGTGTTTAATATTCTCTTTGCTTTCTCTCATAATTCTTATTTTATAGTTAGTTACATTATTATCATAAAACGAAAACACGTAATGGGTACATCATTCAGTTGTGTTCTTTATTCTTATCAGGACCGTCAAAGAATGGTTATcaac                               >  2:4158741/1‑149 (MQ=255)

                                                                                                                       aaTAGTGTTTAATATTCTCTTTGCTTTCGCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCAGTTGTGTTCTTTATTCTTAGCCGGACCGTCAAAGCATGGTTATccacc                              <  1:1836285/149‑1 (MQ=255)

                                                                                                                       aaTAGTGTTTAATATTCTCTTTGCTTTCGCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCAGTTGTGTTCTTTATTCTTAGCCGGACCGTCAAAGCATGGTTATccacc                              <  1:3376962/149‑1 (MQ=255)

                                                                                                                       aaTAGTGTTTAATATTCTCTTTGCTTTCGCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCAGTTGTGTTCTTTATTCTTAGCCGGACCGTCAAAGCATGGTTATccacc                              >  1:4239136/1‑149 (MQ=255)

                                                                                                                       aaTAGTGTTTAATATTCTCTTTGCTTTCGCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCAGTTGTGTTCTTTATTCTTAGCCGGACCGTCAAAGCATGGTTATccacc                              >  1:1090796/1‑149 (MQ=255)

                                                                                                                        aTAGTGTTTAATATTCTCTTTGCTTTCGCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCAGTTGTGTTCTTTATTCTTAGCCGGACCGTCAAAGCATGGTTATccacca                             <  2:2300983/149‑1 (MQ=255)

                                                                                                                        aTAGTGTTTAATATTCTCTTTGCTTTCGCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCAGTTGTGTTCTTTATTCTTAGCCGGACCGTCAAAGCATGGTTATccacca                             >  1:3025412/1‑149 (MQ=255)

                                                                                                                        aTAGTGTTTAATATTCTCTTTGCTTTCGCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCAGTTGTGTTCTTTATTCTTAGCCGGACCGTCAAAGCATGGTTATccacca                             >  1:2537049/1‑149 (MQ=255)

                                                                                                                         tAGTGTTTAATATTCTCTTTGCTTTCGCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCAGTTGTGTTCTTTATTCTTAGCCGGACCGTCAAAGCATGGTTATCCACCAg                            <  2:1009777/149‑1 (MQ=255)

                                                                                                                           gtgtTTAATATTCTCTTTGCTTTCGCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCAGTTGTGTTCTTTATTCTTAGCCGGACCGTCAAAGCATGGTTATCCACCAGTa                          >  1:4002670/1‑149 (MQ=255)

                                                                                                                           gtgtTTAATATTCTCTTTGCTTTCGCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCAGTTGTGTTCTTTATTCTTAGCCGGACCGTCAAAGCATGGTTATCCACCAGTa                          >  2:274533/1‑149 (MQ=255)

                                                                                                                             gtTTAATATTCTCTTTGCTTTCGCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCAGTTGTGTTCTTTATTCTTAGCCGGACCGTCAAAGCATGGTTATCCACCAGTAat                        <  1:3034774/149‑1 (MQ=255)

                                                                                                                              tttAATATTCTCTTTGCTTTCGCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCAGTTGTGTTCTTTATTCTTAGCCGGACCGTCAAAGCATGGTTATCCACCAGTAata                       >  1:2243783/1‑149 (MQ=255)

                                                                                                                               ttAATATTCTCTTTGCTTTCGCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCAGTTGTGTTCTTTATTCTTAGCCGGACCGTCAAAGCATGGTTATCCACCAGTAatat                      <  1:2343896/149‑1 (MQ=255)

                                                                                                                                 aaTATTCTCTTTTCTTTCTCTCATAATTCTTATTTTATCGTTAGTTACATTATGATCATAAACCGAAACCACGTAATGGGTACATCCTTCAGTTGTGTTCTTTATTCTTAGCCGGACCGTCAAAGCATGGTTATCCACCAGTAATATgg                    <  2:3316521/149‑1 (MQ=255)

                                                                                                                                   tatTCTCTTTGCTTTCGCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCAGTTGTGTTCTTTATTCTTAGCCGGACCGTCAAAGCATGGTTATCCACCAGTAATATGGCt                  >  1:3658717/1‑149 (MQ=255)

                                                                                                                                     ttCTCTTTGCTTTCGCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCAGTTGTGTTCTTTATTCTTAGCCGGACCGTCAAAGCATGGTTATCCACCAGTAATATGGCTGc                >  1:4702521/1‑149 (MQ=255)

                                                                                                                                       ctctTTGCTTTCGCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCAGTTGTGTTCTTTATTCTTAGCCGGACCGTCAAAGCATGGTTATCCACCAGTAATATGGCTGCCa              >  2:1924372/1‑149 (MQ=255)

                                                                                                                                         ctTTGCTTTCGCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCAGTTGTGTTCTTTATTCTTAGCCGGACCGTCAAAGCATGGTTATCCACCAGTAATATGGCTGCCATa            >  2:5022279/1‑149 (MQ=255)

                                                                                                                                                ttCGCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCAGTTGTGTTCTTTATTCTTAGCCGGACCGTCAAAGCATGGTTATCCACCAGTAATATGGCTGCCATAACACTGa     >  1:2638464/1‑149 (MQ=255)

                                                                                                                                                ttCGCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCAGTTGTGTTCTTTATTCTTAGCCGGACCGTCAAAGCATGGTTATCCACCAGTAATATGGCTGCCATAACACTGa     <  2:3189462/149‑1 (MQ=255)

                                                                                                                                                   gCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCAGTTGTGTTCTTTATTCTTAGCCGGACCGACAAAGCATGGTTATCCACCAGTAATATGGCTGCCATAACACTGATGc  >  2:4456258/1‑149 (MQ=255)

                                                                                                                                                   gCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCAGTCGTGTTCTTTATTCTTAGCCGGACCGTCAAAGCATGGTTATCCACCAGAAATATGGCTGCCATAACACTGATGc  >  2:4895821/1‑149 (MQ=255)

TTTTAACATCAATAGCTGTTCTTTCGGAAGTGCTGGAATTATTGCAACAGATTGATTAATAACATGATCATTTGACCATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCGCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCAGTTGTGTTCTTTATTCTTAGCCGGACCGTCAAAGCATGGTTATCCACCAGTAATATGGCTGCCATAACACTGATGC  >  CP009273/4211429‑4211724

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`