BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	4,212,014	G→T	6.5%	F134L (TTC→TTA)	arpA ←	ankyrin repeat protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	4,212,014	0	G	T	6.5%	961.8 / 31.8	253	F134L (TTC→TTA)	arpA	ankyrin repeat protein
Reads supporting (aligned to +/- strand): ref base G (105/131); new base T (4/13); total (109/144)
Fisher's exact test for biased strand distribution p-value = 1.28e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 262 total aligned reads displayed.

TTTCAGCTGAAGAGTAATCATCTTGATGGGGTTTGTAATCAACATTCTGATAAATATCATTAATATATTGTGCAGCAATGTGTCGGCATACTATACGGTTTTGGGGTCGCCCCGGAAAATAACATTGACCATTAAGTTTAATTCTTGGGAATTCTTTTTCTTTTCTATCTGATTTTAGGTTTAATTCTTGTTTATCAAGAGTGTCTGAGCGTTGAGGTAAAATTTCCATTAGGTATTTTTCAAATATTGGGGCAATGGTTTGAGTAAGTGTCGTGTTTTCGTTTTTATTAATTATCG  >  CP009273/4211866‑4212162

tttCAGCTGAAGAGTAATCATCTTGATGGGGTTTGTAATCAACATTCTGATAAATATCATTAATATATTGTGCAGCAATGTGTCGGCATACTATACGGTTTTGGGGTCGCCCCGGAAAATAACATTGACCATTAAGTTTAATTCTTggg                                                                                                                                                      <  2:1847422/149‑1 (MQ=255)

   cAGCTGAAGAGTAATCATCTTGATGGGGTTTGTAATCAACATTCTGATAAATATCATTAATATATTGTGCAGCAATGTGTCGGCATACTATACGGTTTTGGGGTCGCCCCGGAAAATAACATTGACCATTAAGTTTAATTCTTGGGAAt                                                                                                                                                   <  2:1554607/149‑1 (MQ=255)

    aGCTGAAGAGTAATCATCTTGATGGGGTTTGTAATCAACATTCTGATAAATATCATTAATATATTGTGCAGCAATGTGTCGGCATACTATACGGTTTTGGGGTCACCCCGGAAAATAACATTGACCATTAAGTTTAATTCTTGGGAAtt                                                                                                                                                  >  2:4807342/1‑149 (MQ=255)

      cTGAAGAGTAATCATCTTGATGGGGTTTGTAATCAACATTCTGATAAATATCATTAATATATTGTGCAGCAATGTGTCGGCATACTATACGGTTTTGGGGTCGCCCCGGAAAATAACATTGACCATTAAGTTTAATTCTTGGGAAttct                                                                                                                                                <  1:4204319/149‑1 (MQ=255)

        gAAGAGTAATCATCTTGATGGGGTTTGTAATCAACATTCTGATAAATATCATTAATATATTGTGCAGCAATGTGTCGGCATACTATACGGTTTTGGGGTCGCCCCGGAAAATAACATTGACCATTAAGTTTAATTCTTGGGAAttcttt                                                                                                                                              <  2:4347851/149‑1 (MQ=255)

          agagTAATCATCTTGATGGGGTTTGTAATCAACATTCTGATAAATATCATTAATATATTGTGCAGCAATGTGTCGGCATACTATACGGTTTTGGGGTCGCCCCGGAAAATAACATTGACCATTAAGTTTAATTCTTGGGAAttcttttt                                                                                                                                            >  2:4533307/1‑149 (MQ=255)

             gTAATCATCTTGATGGGGTTTGTAATCAACATTCTGATAAATATCATTAATATATTGTGCAGCAATGTGTCGGCATACTATACGGTTTTGGGGTCGCCCCGGAAAATAACATTGACCATTAAGTTTAATTCTTGGGAAttctttttctt                                                                                                                                         <  2:1734534/149‑1 (MQ=255)

              tAATCATCTTGCTGGGGTTTGTAATCAACATTCTGATAAATATCATTAATATATTGTGCAGCAATGTGTCGGCATACTATACGGTTTTGGGGTCGCCCCGGAAAATAACATTGACCATTAAGTTTAATTCTTGGGAAttctttttcttt                                                                                                                                        <  2:4880653/149‑1 (MQ=255)

               aaTCATCTTGATGGGGTTTGTAATCAACATTCTGATAAATATCATTAATATATTGTGCAGCAATGTGTCGGCATACTATACGGTTTTGGGGTCGCCCCGGAAAATAACATTGACCATTAAGTTTAATTCTTGGGAATtctttttctttt                                                                                                                                       <  1:1872535/149‑1 (MQ=255)

                 tcatcTTGATGGGGTTTGTAATCAACATTCTGATAAATATCATTAATATATTGTGCAGCAATGTGTCGGCATACTATACGGTTTTGGGGTCGCCCCGGAAAATAACATTGACCATTAAGTTTAATTCTTGGGAATTCTTtttcttttct                                                                                                                                     <  1:386441/149‑1 (MQ=255)

                 tcatcTTGATGGGGTTTGTAATCAACATTCTGATAAATATCATTAATATATTGTGCAGCAATGTGTCGGCATACTATACGGTTTTGGGGTCGCCCCGGAAAATAACATTGACCATTAAGTTTAATTCTTGGGAATTCTTtttcttttct                                                                                                                                     <  1:2392607/149‑1 (MQ=255)

                   atcTTGATGGGGTTTGTAATCAACATTCTGATAAATATCATTAATATATTGTGCAGCAATGTGTCGGCATACTATACGGTTTTGGGGTCGCCCCGGAAAATAACATTGACCATTAAGTTTAATTCTTGGGACTTCTTTTTCTTTTCTAt                                                                                                                                   >  1:2643461/1‑149 (MQ=255)

                   atcTTGATGGGGTTTGTAATCAACATTCTGATAAATATCATTAATATATTGTGCAGCAATGTGTCGGCATACTATACGGTTTTGGGGTCGCCCCGGAAAATAACATTGACCATTAAGTTTAATTCTTGGGAATTCTTTTTCTTTTCTAt                                                                                                                                   <  1:4013541/149‑1 (MQ=255)

                   atcTTGATGGGGTTTGTAATCAACATTCTGATAAATATCATTAATATATTGTGCAGCAATGTGTCGGCATACTATACGGTTTTGGGGTCGCCCCGGAAAATAACATTGACCATTAAGTTTAATTCTTGGGAATTCTTTTTCTTTTCTAt                                                                                                                                   <  1:1383326/149‑1 (MQ=255)

                    tcTTGATGGGGTTTGTAATCAACATTCTGATAAATATCATTAATATATTGTGCAGCAATGTGTCGGCATACTATACGGTTTTGGGGTCGCCCCGGAAAATAGCATTGACCATTAAGTTTAATTCTTGGGAATTCTTTTTCTTTTCTATc                                                                                                                                  <  1:2286569/149‑1 (MQ=255)

                    tcTTGATGGGGTTTGTAATCAACATTCTGATAAATATCATTAATATATTGTGCAGCAATGTGTCGGCATACTATACGGTTTTGGGGTCGCCCCGGAAAATAACATTGACCATTAAGTTTAATTCTTGGTAATTCTTTTTCTTTTCTATc                                                                                                                                  >  1:1417704/1‑149 (MQ=255)

                    tcTTGATGGGGTTTGTAATCAACATTCTGATAAATATCATTAATATATTGTGCAGCAATGTGTCGGCATACTATACGGTTTTGGGGTCGCCCCGGAAAATAACATTGACCATTAAGTTTAATTCTTGGGAATTCTTTTTCTTTTCTATc                                                                                                                                  <  1:3329637/149‑1 (MQ=255)

                    tcTTGATGGGGTTTGTAATCAACATTCTGATAAATATCATTAATATATTGTGCAGCAATGTGTCGGCATACTATACGGTTTTGGGGTCGCCCCGGAAAATAACATTGACCATTAAGTTTAATTCTTGGGAATTCTTTTTCTTTTCTATc                                                                                                                                  <  2:1786480/149‑1 (MQ=255)

                    tcTTGATGGGGTTTGTAATCAACATTCTGATAAATATCATTAATATATTGTGCAGCAATGTGTCGGCATACTATACGGTTTTGGGGTCGCCCCGGAAAATAACATTGACCATTAAGTTTAATTCTTGGGAATTCTTTTTCTTTTCTATc                                                                                                                                  >  1:2846435/1‑149 (MQ=255)

                         aTGGGGTTTGTAATCAACATTCTGATAAATATCATTAATATATTGTGCAGCAATGTGTCGGCATACTATACGGTTTTGGGGTCGCCCCGGAAAATAACATTGACCATTAAGTTTAATTCTTGGGAATTCTTTTTCTTTTCTATCTGAtt                                                                                                                             >  2:3593118/1‑149 (MQ=255)

                           ggggTTTGTAATCAACATTCTGATAAATATCATTAATATATTGTGCAGCAATGTGTCGGCATACTATACGGTTTTGGGGTCGCCCCGGAAAATAACATTGACCATTAAGTTTAATTCTTGGGAATTCTTTTTCTTTTCTATCTGAtttt                                                                                                                           <  2:166507/149‑1 (MQ=255)

                            gggTTTGTAATCAACATTCTGATAAATATCATTAATATATTGTGCAGCAATGTGTCGGCATACTATACGGTTTTGGGGTCGCCCCGGAAAATAACATTGACCATTAAGTTTAATTCTTGGGAATTCTTTTTCTTTTCTATCTGATTTTa                                                                                                                          <  2:2019686/149‑1 (MQ=255)

                               tttGTAATCAACATTCTGATAAATATCATTAATATATTGTGCAGCAATGTGTCGGCATACTATACGGTTTTGGGGTCGCCCCGGAAAATAACATTGACCATTAAGTTTAATTCTTGGGAATTCTTTTTCTTTTCTATCTGATTTTAGGt                                                                                                                       >  2:1169723/1‑149 (MQ=255)

                                ttGTAATCAACATTCTGATAAATATCATTAATATATTGTGCAGCAATGTGTCGGCATACTATACGGTTTTGGGGTCGCCCCGGAAAATAACATTGACCATTAAGTTTAATTCTTGGGAATTCTTTTTCTTTTCTATCTGATTTTAGGtt                                                                                                                      <  2:4314804/149‑1 (MQ=255)

                                ttGTAATCAACATTCTGATAAATATCATTAATATATTGTGCAGCAATGTGTCGGCATACTATACGGTTTTGGGGTCGCCCCGGAAAATAACATTGACCATTAAGTTTAATTCTTGGGAATTCTTTTTCTTTTCTATCTGATTTTAGGtt                                                                                                                      <  2:5021330/149‑1 (MQ=255)

                                   tAATCAACATTCTGATAAATATCATTAATATATTGTGCAGCAATGTGTCGGCATACTATACGGTTTTGGGGTCGCCCCGGAAAATAACATTGACCATTAAGTTTAATTCTTGGGAATTCTTTTTCTTTTCTATCTGATTTTAGGTTTaa                                                                                                                   <  2:2464446/149‑1 (MQ=255)

                                    aaTCAACATTCTGATAAATATCATTAATATATTGTGCAGCAATGTGTCGGCATACTATACGGTTTTGGGGTCGCCCCGGAAAATAACATTGACCATTAAGTTTAATTCTTGGGACTTCTTTTTCTTTTCTATCTGATTTTAGGTTTAAt                                                                                                                  >  1:2064249/1‑149 (MQ=255)

                                     aTCAACATTCTGATAAATATCATTAATATATTGTGCAGCAATGTGTCGGCATACTATACGGTTTTGGGGTCGCCCCGGAAAATAACATTGACCATTAAGTTTAATTCTTGGTAATTCTTTTTCTTTTCTATCTGATTTTAGGTTTAAtt                                                                                                                 >  1:2011224/1‑149 (MQ=255)

                                      tCAACATTCTGATAAATATCATTAATATATTGTGCAGCAATGTGTCGGCATACTATACGGTTTTGGGGTCGCCCCGGAAAATAACATTGACCATTAAGTTTAATTCTTGGGAATTCTTTTTCTTTTCTATCTGATTTTAGGTTTAATTc                                                                                                                <  1:2968718/149‑1 (MQ=255)

                                         aCATTCTGATAAATATCATTAATATATTGTGCAGCAATGTGTCGGCATACTATACGGTTTTGGGGTCGCCCCGGAAAATAACATTGACCATTAAGTTTAATTCTTGGGAATTCTTTTTCTTTTCTATCTGATTTTAGGTTTAATTCTTg                                                                                                             >  1:3152157/1‑149 (MQ=255)

                                                 aTAAATATCATTAATATATTGTGCAGCAATGTGTCGGCATACTATACGGTTTTGGGGTCGCCCCGGAAAATAACATTGACCATTAAGTTTAATTCTTGGGAATTCTTTTTCTTTTCTATCTGATTTTAGGTTTAATTCTTGTTTATCaa                                                                                                     >  2:4570305/1‑149 (MQ=255)

                                                   aaaTATCATTAATATATTGTGCAGCAATGTGTCGGCATACTATACGGTTTTGGGGTCGCCCCGGAAAATAACATTGACCATTAAGTTTAATTCTTGGGAATTCTTTTTCTTTTCTATCTGATTTTAGGTTTAATTCTTGTTTATCAaga                                                                                                   >  1:4779205/1‑149 (MQ=255)

                                                    aaTATCATTAATATATTGTGCAGCAATGTGTCGGCATACTATACGGTTTTGGGGTCGCCCCGGAAAATAACATTGACCATTAAGTTTAATTCTTGGGAATTCTTTTTCTTTTCTATCTGATTTTAGGTTTAATTCTTGTTTATCAagag                                                                                                  <  1:1004047/149‑1 (MQ=255)

                                                    aaTATCATTAATATATTGTGCAGCAATGTGTCGGCATACTATACGGTTTTGGGGTCGCCCCGGAAAATAACATTGACCATTAAGTTTAATTCTTGGGAATTCTTTTTCTTTTCTATCTGATTTTAGGTTTAATTCTTGTTTATCAagag                                                                                                  <  1:1297310/149‑1 (MQ=255)

                                                         cATTAATATATTGTGCAGCAATGTGTCGGCATACTATTCGGTTTTGGGGTCTCCCCGGAAAATAACATTGACCATTAATTTTTATTCTTGGTAATTCTTTTTCTTTTCTATCTGATTTTAGGTTTAATTCTTGTTTATCAAGAGTGTCt                                                                                             <  1:5234771/149‑1 (MQ=255)

                                                         cATTAATATATTGTGCAGCAATGTGTCGGCATACTATACGGTTTTGGGGTCGCCCCGGAAAATAACATTGACCATTAAGTTTAATTCTTGGGAATTCTTTTTCTTTTCTATCTGATTTTAGGTTTAATTCTTGTTTATCAAGAGTGTCt                                                                                             <  2:2603841/149‑1 (MQ=255)

                                                             aaTATATTGTGCAGCAATGTGTCGGCATACTATACGGTTTTGGGGTCGCCCCGGAAAATAACATTGACCATTAAGTTTAATTCTTGGGAATTCTTTTTCTTTTCTATCTGATTTTAGGTTTAATTCTTGTTTATCAAGAGTGTCTGAGc                                                                                         <  2:3353334/149‑1 (MQ=255)

                                                             aaTATATTGTGCAGCAATGTGTCGGCATACTATACGGTTTTGGGGTCGCCCCGGAAAATAACATTGACCATTAAGTTTAATTCTTGGGAATTCTTTTTCTTTTCTATCTGATTTTAGGTTTAATTCTTGTTTATCAAGAGTGTCTGAGc                                                                                         <  1:4082151/149‑1 (MQ=255)

                                                             aaTATATTGTGCAGCAATGTGTCGGCATACTATACGGTTTTGGGGTCGCCCCGGAAAATAACATTGACCATTAAGTTTAATTCTTGGGAATTCTTTTTCTTTTCTATCTGATTTTAGGTTTAATTCTTGTTTATCAAGAGTGTCTGAGc                                                                                         <  2:827729/149‑1 (MQ=255)

                                                              atatatTGTGCAGCAATGTGTCGGCATACTATACGGTTTTGGGGTCGCCCCGGAAAATAACATTGACCATTAAGTTTAATTCTTGGGAATTCTTTTTCTTTTCTATCTGATTTTAGGTTTAATTCTTGTTTATCAAGAGTGTCTGAGCg                                                                                        >  2:5073332/1‑149 (MQ=255)

                                                               tatatTGTGCAGCAATGTGTCGGCATACTATACGGTTTTGGGGTCGCCCCGGAAAATAACATTGACCATTAAGTTTAATTCTTGGGAATTCTTTTTCTTTTCTATCTGATTTTAGGTTTAATTCTTGTTTATCAAGAGTGTCTGAGCGt                                                                                       <  2:4718842/149‑1 (MQ=255)

                                                               tatatTGTGCAGCAATGTGTCGGCATACTATACGGTTTTGGGGTCGCCCCGGAAAATAACATTGACCATTAAGTTTAATTCTTGGGAATTCTTTTTCTTTTCTATCTGATTTTAGGTTTAATTCTTGTTTATCAAGAGTGTCTGAGCGt                                                                                       >  2:300722/1‑149 (MQ=255)

                                                                atatTGTGCAGCAATGTGTCGGCATACTATACGGTTTTGGGGTCGCCCCGGAAAATAACATTGACCATTAAGTTTAATTCTTGGGAATTCTTTTTCTTTTCTATCTGATTTTAGGTTTAATTCTTGTTTATCAAGAGTGTCTGAGCGtt                                                                                      >  2:3630112/1‑149 (MQ=255)

                                                                 tatTGTGCAGCAATGTGTCGGCATACTATACGGTTTTGGGGTCGCCCCGGAAAATAACATTGACCATTAAGTTTAATTCTTGGGAATTCTTTTTCTTTTCTATCTGATTTTAGGTTTAATTCTTGTTTATCAAGAGTGTCTGAGCGTTg                                                                                     >  2:234379/1‑149 (MQ=255)

                                                                  atTGTGCAGCAATGTGTCGGCATACTATACGGTTTTGGGGTCGCCCCGGAAAATAACATTGACCATTAAGTTTAATTCTTGGGAATTCTTTTTCTTTTCTATCTGATTTTAGGTTTAATTCTTGTTTATCAAGAGTGTCTGAGCGTTGa                                                                                    <  1:4908944/149‑1 (MQ=255)

                                                                       gcagcaATGTGTCGGCATACTATACGGTTTTGGGGTCGCCCCGGAAAATAACATTGACCATTAAGTTTAATTCTTGGGAATTCTTTTTCTTTTCTATCTGATTTTAGGTTTAATTCTTGTTTATCAAGAGTGTCTGAGCGTTGAGGTaa                                                                               <  1:4100299/149‑1 (MQ=255)

                                                                         agcaATGTGTCGTCATACTAAACGGTTTTGGGGTCGCCCCGGAAAATAAAATTGACCATTAAGTTTAATTCTTGGGAATTCTTTTTCTTTTCTATCTGATTTTAGGTTTAATTCTTGTTTATCAAGAGTGTCTGAGCGTTGAGGTaaaa                                                                             <  2:3717380/149‑1 (MQ=255)

                                                                         agcaATGTGTCGGCATACTATACGGTTTTGGGGTCGCCCCGGAAAATAACATTGACCATTAAGTTTAATTCTTGGGAATTCTTTTTCTTTTCTATCTGATTTTAGGTTTAATTCTTGTTTATCAAGAGTGTCTGAGCGTTGAGGTaaaa                                                                             <  1:2978791/149‑1 (MQ=255)

                                                                          gcaATGTGTCGGCATACTATACGGTTTTGGGGTCGCCCCGGAAAATAACATTGACCATTAAGTTTAATTCTTGGGAATTCTTTTTCTTTTCTATCTGATTTTAGGTTTAATTCTTGTTTATCAAGAGTGTCTGAGCGTTGAGGTAAAAt                                                                            >  1:1538591/1‑149 (MQ=255)

                                                                             aTGTGTCGGCATACTATACGGTTTTGGGGTCGCCCCGGAAAATAACATTGACCATTAAGTTTAATTCTTGGGAATTCTTTTTCTTTTCTATCTGATTTTAGGTTTAATTCTTGTTTATCAAGAGTGTCTGAGCGTTGAGGTAAAATTTc                                                                         >  1:1947543/1‑149 (MQ=255)

                                                                             aTGTGTCGGCATACTATACGGTTTTGGGGTCGCCCCGGAAAATAACATTGACCATTAAGTTTAATTCTTGGGAATTCTTTTTCTTTTCTATCTGATTTTAGGTTTAATTCTTGTTTATCAAGAGTGTCTGAGCGTTGAGGTAAAATTTc                                                                         >  2:3742638/1‑149 (MQ=255)

                                                                             aTGTGTCGGCATACTATACGGTTTTGGGGTCGCCCCGGAAAATAACATTGACCATTAAGTTTAATTCTTGGGAATTCTTTTTCTTTTCTATCTGATTTTAGGTTTAATTCTTGTTTATCAAGAGTGTCTGAGCGTTGAGGTAAAATTTc                                                                         >  1:1947560/1‑149 (MQ=255)

                                                                             aTGTGTCGGCATACTATACGGTTTTGGGGTCGCCCCGGAAAATAACATTGACCATTAAGTTTAATTCTTGGGAATTCTTTTTCTTTTCTATCTGATTTTAGGTTTAATTCTTGTTTATCAAGAGTGTCTGAGCGTTGAGGTAAAATTTc                                                                         >  1:1947665/1‑149 (MQ=255)

                                                                                tgtCGGCATACTATACGGTTTTGGGGTCGCCCCGTAAAATAACATTGACCATTAAGTTTAATTCTTGGGAATTCTTTTTCTTTTCTATCTGATTTTAGGTTTAATTCTTGTTTATCAAGAGTGTCTGAGCGTTGAGGTAAAATTTCCAt                                                                      <  2:3924389/149‑1 (MQ=255)

                                                                                  tCGGCATACTATACGGTTTTGGGGTCGCCCCGGAAAATAACATTGACCATTAAGTTTAATTCTTGGGAATTCTTTTTCTTTTCTATCTGATTTTAGGTTTAATTCTTGTTTATCAAGAGTGTCTGAGCGTTGAGGTAAAATTTCCATTa                                                                    >  1:4344491/1‑149 (MQ=255)

                                                                                  tCGGCATACTATACGGTTTTGGGGTCGCCCCGGAAAATAACATTGACCATTAAGTTTAATTCTTGGGAATTCTTTTTCTTTTCTATCTGATTTTAGGTTTAATTCTTGTTTATCAAGAGTGTCTGAGCGTTGAGGTAAAATTTCCATTa                                                                    >  2:3507806/1‑149 (MQ=255)

                                                                                     gCATACTATACGGTTTTGGGGTCGCCCCGGAAAATAACATTGACCATTAAGTTTAATTCTTGGGAATTCTTTTTCTTTTCTATCTGATTTTAGGTTTAATTCTTGTTTATCAAGAGTGTCTGTGCGTTGAGGTAAAATTTCCATTAGGt                                                                 >  2:3203114/1‑149 (MQ=255)

                                                                                     gCATACTATACGGTTTTGGGGTCGCCCCGGAAAATAACATTGACCATTAAGTTTAATTCTTGGGAATTCTTTTTCTTTTCTATCTGATTTTAGGTTTAATTCTTGTTTATCAAGAGTGTCTGAGCGTTGAGGTAAAATTTCCATTAGGt                                                                 >  2:8721/1‑149 (MQ=255)

                                                                                     gCATACTATACGGTTTTGGGGTCGCCCCGGAAAATAACATTGACCATTAAGTTTAATTCTTGGGAATTCTTTTTCTTTTCTATCTGATTTTAGGTTTAATTCTTGTTTATCAAGAGTGTCTGAGCGTTGAGGTAAAATTTCCATTAGGt                                                                 >  2:2486061/1‑149 (MQ=255)

                                                                                        tACTATACGGTTTTGGGGTCGCCCCGGAAAATAACCTTGACCATTAAGTTTAATTCTTGGGAATTCTTTTTCTTTTCTATCTGATTTTAGGTTTAATTCTTGTTTATCAAGAGTGTCTGAGCGTTGAGGTAAAATTTCCATTAGGTAtt                                                              <  2:3814613/149‑1 (MQ=255)

                                                                                         aCTATACGGTTTTGGGGTCGCCCCGGAAAATAACATTGACCATTAAGTTTAATTCTTGGGAATTCTTTTTCTTTTCTATCTGATTTTAGGTTTAATTCTTGTTTATCAAGAGTGTCTGAGCGTTGAGGTAAAATTTCCATTAGGTAttt                                                             <  1:5143362/149‑1 (MQ=255)

                                                                                           tataCGGTTTTGGGGTCGCCCCGGAAAATAACATTGACCATTAAGTTTAATTCTTGGGAATTCTTTTTCTTTTCTATCTGATTTTAGGTTTAATTCTTGTTTATCAAGAGTGTCTGAGCGTTGAGGTAAAATTTCAATTAGGTAttttt                                                           >  1:2781331/1‑149 (MQ=255)

                                                                                            ataCGGTTTTGGGGTCGCCCCGGAAAATAACATTGACCATTAAGTTTAATTCTTGGGAATTCTTTTTCTTTTCTATCTGATTTTAGGTTTAATTCTTGTTTATCAAGAGTGTCTGAGCGTTGAGGTAAAATTTCCATTAGGTATTTTTc                                                          <  1:803566/149‑1 (MQ=255)

                                                                                                ggTTTTGGGGTCGCCCCGGAAAATAACATTGACCATTAAGTTTAATTCTTGGGAATTCTTTTTCTTTTCTATCTGATTTTAGGTTTAATTCTTGTTTATCAAGAGTGTCTGAGCGTTGAGGTAAAATTTCCATTAGGTATTTTTCAAat                                                      <  1:1571241/149‑1 (MQ=255)

                                                                                                 gTTTTGGGGTCGCCCCGGAAAATAACATTGACCATTAAGTTTAATTCTTGGGAATTCTTTTTCTTTTCTATCTGATTTTAGGTTTAATTCTTGTTTATCAAGAGTGTCTGAGCGTTGAGGTAAAATTTCCATTAGGTATTTTTCAAata                                                     >  2:3434300/1‑149 (MQ=255)

                                                                                                 gTTTTGGGGTCGCCCCGGAAAATAACATTGACCATTAAGTTTAATTCTTGGGAATTCTTTTTCTTTTCTATCTGATTTTAGGTTTAATTCTTGTTTATCAAGAGTGTCTGAGCGTTGAGGTAAAATTTCCATTAGGTATTTTTCAAata                                                     >  2:523984/1‑149 (MQ=255)

                                                                                                   tttGGGGTCGCCCCGGAAAATAACATTGACCATTAAGTTTAATTCTTGGGAATTCTTTTTCTTTTCTATCTGATTTTAGGTTTAATTCTTGTTTATCAAGAGTGTCTGAGCGTTGAGGTAAAATTTCCATTAGGTATTTTTCAAATAtt                                                   <  1:4444512/149‑1 (MQ=255)

                                                                                                    ttGGGGTCGCCCCTGAAAATAACATTGACCATTAAGTTTAATTCTTGGGAATTCTTTTTCTTTTCTATCTGATTTTAGGTTTAATTCTTGTTTATCAAGTGTGTCTGAGCGTTGAGGTAAAATTTCCATTAGGTATTTTTCAAATATTg                                                  <  1:3530460/149‑1 (MQ=255)

                                                                                                    ttGGGGTCGCCCCGGAAAATAACATTGACCATTAAGTTTAATTCTTGGTAATTCTTTTTCTTTTCTATCTGATTTTAGGTTTAATTCTTGTTTATCAAGAGTGTCTGAGCGTTGAGGTAAAATTTCCATTAGGTATTTTTCAAATATTg                                                  <  2:4254312/149‑1 (MQ=255)

                                                                                                    ttGGGGTCGCCCCGGAAAATAACATTGACCATTAAGTTTAATTCTTGGGAATTCTTTTTCTTTTCTATCTGATTTTAGGTTTAATTCTTGTTTATCAAGAGTGTCTGAGCGTTGAGGTAAAATTTCCATTAGGTATTTTTCAAATATTg                                                  <  2:1219426/149‑1 (MQ=255)

                                                                                                      ggggTCGCCCCGGAAAATAACATTGACCATTAAGTTTAATTCTTGGGAATTCTTTTTCTTTTCTATCTGATTTTAGGTTTAATTCTTGTTTATCAAGAGTGTCTGAGCGTTGAGGTAAAATTTCCATTAGGTATTTTTCAAATATTggg                                                <  1:4562452/149‑1 (MQ=255)

                                                                                                      ggggTCGCCCCGGAAAATAACATTGACCATTAAGTTTAATTCTTGGGAATTCTTTTTCTTTTCTATCTGATTTTAGGTTTAATTCTTGTTTATCAAGAGTGTCTGAGCGTTGAGGTAAAATTTCCATTAGGTATTTTTCAAATATTggg                                                <  1:2347455/149‑1 (MQ=255)

                                                                                                             ccccGGAAAATAACATTGACCATTAAGTTTAATTCTTGGGAATTCTTTTTCTTTTCTATCTGATTTTAGGTTTAATTCTTGTTTATCAAGAGTGTCTGAGCGTTGAGGTAAAATTTCCATTAGGTATTTTTCAAATATTGGGGCAATgg                                         >  2:1455382/1‑149 (MQ=255)

                                                                                                               ccGGAAAATAACATTGACCATTAAGTTTAATTCTTGGGAATTCTTTTTCTTTTCTATCTGATTTTAGGTTTAATTCTTGTTTATCAAGAGTGTCTGAGCGTTGAGGTAAAATTTCCATTAGGTATTTTTCAAATATTGGGGCAATGGtt                                       <  1:1724936/149‑1 (MQ=255)

                                                                                                                  gAAAATAACATTGACCATTAAGTTTAATTCTTGGGAATTCTTTTTCTTTTCTATCTGATTTTAGGTTTAATTCTTGTTTATCAAGAGTGTCTGAGCGTTGAGGTAAAATTTCCATTAGGTATTTTTCAAATATTGGGGCAATGGTTTGa                                    >  1:4835819/1‑149 (MQ=255)

                                                                                                                   aaaaGAACATTGACCATTAAGTTTAATTCTTGGGAATTCTTTTTCTTTTCTATCTGATTTTAGGTTTAATTCTTGTTTATCAAGAGTGTCTGAGCGTTGAGGTAAAATTTCCATTAGGTATTTTTCAAATATTGGGGCAATGGTTTGAg                                   <  2:495212/149‑1 (MQ=255)

                                                                                                                      aTAACATTGACCATTAAGTTTAATTCTTGGGAATTCTTTTTCTTTTCTATCTGATTTTAGGTTTAATTCTTGTTTATCAAGAGTGTCTGAGCGTTGAGGTAAAATTTCCATTAGGTATTTTTCAAATATTGGGGCAATGGTTTGAGTaa                                <  1:4664092/149‑1 (MQ=255)

                                                                                                                       tAACATTGACCATTAAGTTTAATTCTTGGTAATTCTTTTTCTTTTCTATCTGATTTTAGGTTTAATTCTTGTTTATCAAGAGTGTCTGAGCGTTGAGGTAAAATTTCCATTAGGTATTTTTCAAATATTGGGGCAATGGTTTGAGTAAg                               <  1:1688120/149‑1 (MQ=255)

                                                                                                                       tAACATTGACCATTAAGTTTAATTCTTGGGAATTCTTTTTCTTTTCTATCTGATTTTAGGTTTAATTCTTGTTTATCAAGAGTGTCTGAGCGTTGAGGTAAAATTTCCATTAGGTATTTTTCAAATATTGGGGCAATGGTTTGAGTAAg                               <  1:3410557/149‑1 (MQ=255)

                                                                                                                        aaCATTGACCATTAAGTTTAATTCTTGGGAATTCTTTTTCTTTTCTATCTGATTTTAGGTTTAATTCTTGTTTATCAAGAGTGTCTGAGCGTTGAGGTAAAATTTCCATTAGGTATTTTTCAAATATTGGGGCAATGGTTTGAGTAAgt                              >  2:363744/1‑149 (MQ=255)

                                                                                                                        aaCATTGACCATTAAGTTTAATTCTTGGGAATTCTTTTTCTTTTCTATCTGATTTTAGGTTTAATTCTTGTTTATCAAGAGTGTCTGAGCGTTGAGGTAAAATTTCCATTAGGTATTTTTCAAATATTGGGGCAATGGTTTGAGTAAgt                              <  2:832880/149‑1 (MQ=255)

                                                                                                                         aCATTGACCATTAAGTTTAATTCTTGGGAATTCTTTTTCTTTTCTATCTGATTTTAGGTTTAATTCTTGTTTATCAAGAGTGTCTGAGCGTTGAGGTAAAATTTCCATTAGGTATTTTTCAAATATTGGGGCAATGGTTTGAGTAAgtg                             <  2:2166780/149‑1 (MQ=255)

                                                                                                                         aCATTGACCATTAAGTTTAATTCTTGGGAATTCTTTTTCTTTTCTATCTGATTTTAGGTTTAATTCTTGTTTATCAAGAGTGTCTGAGCGTTGAGGTAAAATTTCCATTAGGTATTTTTCAAATATTGGGGCAATGGTTTGAGTAAgtg                             >  1:1135571/1‑149 (MQ=255)

                                                                                                                          cATTGACCATTAAGTTTAATTCTTGGGAATTCTTTTTCTTTTCTATCTGATTTTAGGTTTAATTCTTGTTTATCAAGAGTGTCTGAGCGTTGAGGTAAAATTTCCATTAGGTATTTTTCAAATATTGGGGCAATGGTTTGAGTAAgtgt                            <  2:5159997/149‑1 (MQ=255)

                                                                                                                          cATTGACCATTAAGTTTAATTCTTGGGAATTCTTTTTCTTTTCTATCTGATTTTAGGTTTAATTCTTGTTTATCAAGAGTGTCTGAGCGTTGAGGTAAAATTTCCATTAGGTATTTTTCAAATATTGGGGCAATGGTTTGAGTAAgtgt                            <  1:5194136/149‑1 (MQ=255)

                                                                                                                          cATTGACCATTAAGTTTAATTCTTGGGAATTCTTTTTCTTTTCTATCTGATTTTAGGTTTAATTCTTGTTTATCAAGAGTGTCTGAGCGTTGAGGTAAAATTTCCATTAGGTATTTTTCAAATATTGGGGCAATGGTTTGAGTAAgtgt                            <  1:319367/149‑1 (MQ=255)

                                                                                                                           aTTGACCATTAAGTTTAATTCTTGGGAATTCTTTTTCTTTTCTATCTGATTTTAGGTTTAATTCTTGTTTATCAAGAGTGTCTGAGCGTTGAGGTAAAATTTCCATTAGGTATTTTTCAAATATTGGGGCAATGGTTTGAGTAAGTGTc                           >  1:3620656/1‑149 (MQ=255)

                                                                                                                             tGACCATTAAGTTTAATTCTTGGGACTTCTTTTTCTTTTCTATCTGATTTTAGGTTTAATTCTTGTTTATCAAGAGTGTCTGAGCGTTGAGGTAAAATTTCCATTAGGTATTTTTCAAATATTGGGGCAATGGTTTGAGTAAGTGTCgt                         >  1:3057482/1‑149 (MQ=255)

                                                                                                                             tGACCATTAAGTTTAATTCTTGGGAATTCTTTTTCTTTTCTATCTGATTTTAGGTTTAATTCTTGTTTATCAAGAGTGTCTGAGCGTTGAGGTAAAATTTCCATTAGGTATTTTTCAAATATTGGGGCAATGGTTTGAGTAAGTGTCgt                         >  1:4156193/1‑149 (MQ=255)

                                                                                                                              gACCATTTAGTTTAATTCTTGGGAATTCTTTTTCTTTTCTATCTGATTTTAGGTTTAATTCTTGTTTATCAAGAGTGTCTGAGCGTTGAGGTAAAATTTCCATTAGGTATTTTTCAAATATTGGGGCAATGGTTTGAGTAAGTGTCgtg                        <  1:4417205/149‑1 (MQ=255)

                                                                                                                               aCCATTAAGTTTAATTCTTGGGAATTCTTTTTCTTTTCTATCTGATTTTAGGTTTAATTCTTGTTTATCAAGAGTGTCTGAGCGTTGAGGTAAAATTTCCATTAGGTATTTTTCAAATATTGGGGCAATGGTTTGAGTAAGTGTCgtgt                       <  2:3159342/149‑1 (MQ=255)

                                                                                                                                ccATTAAGTTTAATTCTTGGGAATTCTTTTTCTTTTCTATCTGATTTTAGGTTTAATTCTTGTTTATCAAGAGTGTCTGAGCGTTGAGGTAAAATTTCCATTAGGTATTTTTCAAATATTGGGGCAATGGTTTGAGTAAGTGTCGTGtt                      <  2:2835946/149‑1 (MQ=255)

                                                                                                                                   ttAAGTTTAATTCTTGGGAATTCTTTTTCTTTTCTATCTGATTTTAGGTTTAATTCTTGTTTATCAAGAGTGTCTGAGCGTTGAGGTAAAATTTCCATTAGGTATTTTTCAAATATTGGGGCAATGGTTTGAGTAAGTGTCGTGTTTTc                   <  1:3228312/149‑1 (MQ=255)

                                                                                                                                        tttATTTCTTGTTAATTCTTTTTCTTTTCTATCTGATTTTAGGTTTAATTCTTGTTTATCAAGAGTGTCTGAGAGTTGAGGTAAAATTTCCATTAGGTATTTTTCAAATATTGGGGCAATGGTTTGAGTAAGTGTCGTGTTTTCGtttt              <  2:4038653/149‑1 (MQ=255)

                                                                                                                                              tCTTGGGAATTCTTTTTCTTTTCTATCTGATTTTAGGTTTAATTCTTGTTTATCAAGAGTGTCTGAGCGTTGAGGTAAAATTTCCATTAGGTATTTTTCAAATATTGGGGCAATGGTTTGAGTAAGTGTCGTGTTTTCGTTTTTattaa        <  1:947235/149‑1 (MQ=255)

                                                                                                                                              tCTTGGGAATTCTTTTTCTTTTCTATCTGATTTTAGGTTTAATTCTTGTTTATCAAGAGTGTCTGAGCGTTGAGGTAAAATTTCCATTAGGTATTTTTCAAATATTGGGGCAATGGTTTGAGTAAGTGTCGTGTTTTCGTTTTTattaa        <  1:3774368/149‑1 (MQ=255)

                                                                                                                                               cTTGGGAATTCTTTTTCTTTTCTATCTGATTTTAGGTTTAATTCTTGTTTATCAAGAGTGTCTGAGCGTTGAGGTAAAATTTCCATTAGGTATTTTTCAAATATTGGGGCAATGGTTTGAGTAAGTGTCGTGTTTTCGTTTTTattaat       >  2:1826527/1‑149 (MQ=255)

                                                                                                                                                  gggAATTCTTTTTCTTTTCTATCTGATTTTAGGTTTAATTCTTGTTTATCAAGAGTGTCTGAGCGTTGAGGTAAAATTTCCATTAGGTATTTTTCAAATATTGGGGCAATGGTTTGAGTAAGTGTCGTGTTTTCGTTTTTATTAATTAt    >  2:3688948/1‑149 (MQ=255)

                                                                                                                                                  gggAATTCTTTTTCTTTTCTATCTGATTTTAGGTTTAATTCTTGTTTATCAAGAGTGTCTGAGCGTTGAGGTAAAATTTCCATTAGGTATTTTTCAAATATTGGGGCAATGGTTTGAGTAAGTGTCGTGTTTTCGTTTTTATTAATTAt    <  2:957240/149‑1 (MQ=255)

                                                                                                                                                    gAATTCTTTTTCTTTTCTATCTGATTTTAGGTTTAATTCTTGTTTATCAAGAGTGTCTGAGCGTTGAGGTAAAATTTCCATTAGGTATTTTTCAAATATTGGGGCAATGGTTTGAGTAAGTGTCGTGTTTTCGTTTTTATTAATTATCg  >  2:1049466/1‑149 (MQ=255)

TTTCAGCTGAAGAGTAATCATCTTGATGGGGTTTGTAATCAACATTCTGATAAATATCATTAATATATTGTGCAGCAATGTGTCGGCATACTATACGGTTTTGGGGTCGCCCCGGAAAATAACATTGACCATTAAGTTTAATTCTTGGGAATTCTTTTTCTTTTCTATCTGATTTTAGGTTTAATTCTTGTTTATCAAGAGTGTCTGAGCGTTGAGGTAAAATTTCCATTAGGTATTTTTCAAATATTGGGGCAATGGTTTGAGTAAGTGTCGTGTTTTCGTTTTTATTAATTATCG  >  CP009273/4211866‑4212162

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`