BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	4,495,002	C→A	11.9%	F376L (TTC→TTA)	yjhB →	putative transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	4,495,002	0	C	A	11.9%	901.0 / 77.5	261	F376L (TTC→TTA)	yjhB	putative transporter
Reads supporting (aligned to +/- strand): ref base C (113/117); new base A (22/9); total (135/126)
Fisher's exact test for biased strand distribution p-value = 3.39e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.68e-01

Only 100 of 263 total aligned reads displayed.

CCAAAATTTATATATGATTACTTTCCAACAAAATTAAGAGGATTAGGGACCGGTCTTATTTATAACTTAGGGGCAACTGGAGGAATGGCCGCACCTGTATTAGCTACATACATTTCAGGATATTATGGCTTAGGTGTTTCATTATTCATTGTTACGGTTGCATTCTCTGCCTTATTAATTTTGTTAGTTGGTTTTGATATTCCAGGTAAAATTTATAAACTATCCGTGGCTAAATGATTGGAGGCTTTATGATTAATTATGGCGTTGTTGGTGTTGGATACTTTGGCGCTGAATT  >  CP009273/4494856‑4495150

 cAAAATTTATATATGATTACTTTCCAACAAAATTAAGAGGATTAGGGACCGGTCTTATTTATAACTTAGGGGCAACTGGAGGAATGGCCGCACCTGTATTAGCTACATACATTTCAGGATATTATGGCTTAGGTGTTTCATTATTCAtt                                                                                                                                                   <  2:1799661/149‑1 (MQ=255)

 cAAAATTTATATATGATTACTTTCCAACAAAATTAAGAGGATTAGGGACCGGTCTTATTTATAACTTAGGGGCAACTGGAGGAATGGCCGCACCTGTATTAGCTACATACATTTCAGGATATTATGGCTTAGGTGTTTCATTATTCAtt                                                                                                                                                   >  1:1859895/1‑149 (MQ=255)

   aaaTTTATATATGATTACTTTCCAACAAAATTAAGAGGATTAGGGACCGGTCTTATTTATAACTTAGGGGCAACTGGAGGAATGGCCGCACCTGTATTAGCTACATACATTTCAGGATATTATGGCTTAGGTGTTTCATTATTCATTGt                                                                                                                                                 <  1:208374/149‑1 (MQ=255)

     aTTTATATATGATTACTTTCCAACAAAATTAAGAGGATTAGGGACCGGTCTTATTTATAACTTAGGGGCAACTGGAGGAATGGCCGCACCTGTATTAGCTACATACATTTCAGGATATTATGGCTTAGGTGTTTCATTATTCATTGTTa                                                                                                                                               >  2:3507844/1‑149 (MQ=255)

     aTTTATATATGATTACTTTCCAACAAAATTAAGAGGATTAGGGACCGGTCTTATTTATAACTTAGGGGCAACTGGAGGAATGGCCGCACCTGTATTAGCTACATACATTTCAGGATATTATGGCTTAGGTGTTTCATTATTAATTGTTa                                                                                                                                               >  1:737877/1‑149 (MQ=255)

      tttATATATGATTACTTTACAACAAAATTAAGAGGATTAGGGACAGGTCGTATTTATAACTTAGGGGCAACTGGAGGAATGGTCGCACCTGTATTAGCTACATACATTTCAGGATATTATGGCTTAGGTGTTTAATTATTCATTTTTAc                                                                                                                                              >  2:2505459/1‑149 (MQ=255)

       ttATATATGATTACTTTCCAACAAAATTAAGAGGATTAGGGACCGGTCTTATTTATAACTTAGGGGCAACTGGAGGAATGGCCGCACCTGTATTAGCTACATACATTTCAGGATATTATGGCTTAGGTGTTTCATTATTCATTGTTACg                                                                                                                                             >  1:4735455/1‑149 (MQ=255)

         atatatGATTACTTTCCAACAAAATTAAGAGGATTAGGGACCGGTCTTATTTATAACTTAGGGGCAACTGGAGGAATGGCCGCACCTGTATTAGCTACATACATTTCAGGATATTATGGCTTAGGTGTTTCATTATTCATTGTTACGGt                                                                                                                                           >  1:1189558/1‑149 (MQ=255)

            tatGATTACTTTCCAACAAAATTAAGAGGATTAGGGACCGGTCTTATTTATAACTTAGGGGCAACTGGAGGAATGGCCGCACCTGTATTAGCTACATACATTTCAGGATATTATGGCTTAGGTGTTTCATTATTCATTGTTACGGTTGc                                                                                                                                        >  2:661245/1‑149 (MQ=255)

            tatGATTACTTTACAACAAAATTAAGAGGATTAGGGACCGGTCTTATTTATAACTTAGGGGCAACTGGAGGAATGGCCGCACCTGTATTAGCTACATACATTTCAGGATATTATGGCTTAGGTGTTTCATTATTCATTGTTACGGTTGc                                                                                                                                        <  1:1922718/149‑1 (MQ=255)

              tGATTACTTTCCAACAAAATTAAGAGGATTAGGGACCGGTCTTATTTATAACTTAGGGGCAACTGGAGGAATGGCCGCACCTGTATTAGCTACATACATTTCAGGATATTATGGCTTAGGTGTTTCATTATTCATTGTTACGGTTGCAt                                                                                                                                      <  2:3820680/149‑1 (MQ=255)

                aTTACTTTCCAACAAAATTAAGAGGATTAGGGACCGGTCTTATTTATAACTTAGGGGCAACTGGAGGAATGGCCGCACCTGTATTAGCTACATACATTTCAGGATATTATGGCTTAGGTGTTTCATTATTCATTGTTACGGTTGCATtc                                                                                                                                    >  2:875759/1‑149 (MQ=255)

                aTTACTTTCCAACAAAATTAAGAGGATTAGGGACCGGTCTTATTTATAACTTAGGGGCAACTGGAGGAATGGCCGCACCTGTATTAGCTACATACATTTCAGGATATTATGGCTTAGGTGTTTCATTATTCATTGTTACGGTTGCATtc                                                                                                                                    <  1:3871906/149‑1 (MQ=255)

                  tACTTTCCAACAAAATTAAGAGGATTAGGGACCGGTCTTATTTATAACTTAGGGGCAACTGGAGGAATGGCCGCACCTGTATTAGCTACATACATTTCAGGATATTATGGCTTAGGTGTTTCATTATTCATTGTTACTGTTGCATtctc                                                                                                                                  >  2:2903852/1‑149 (MQ=255)

                      ttCCAACAAAATTAAGAGGATTAGGGACCGGTCTTATTTATAACTTAGGGGCAACTGGAGGAATGGCCGCACCTGTATTAGCTACATACATTTCAGGATATTATGGCTTAGGTGTTTCATTATTCATTGTTACGGTTGCATTCTCTGcc                                                                                                                              >  1:2615758/1‑149 (MQ=255)

                      ttCCAACAAAATTAAGAGGATTAGGGACCGGTCTTATTTATAACTTAGGGGCAACTGGAGGAATGGCCGCACCTGTATTAGCTACATACATTTCAGGATATTATGGCTTAGGTGTTTCATTATTCATTGTTACGGTTGCATTCTCTGcc                                                                                                                              <  2:3604809/149‑1 (MQ=255)

                       tCCAACAAAATTAAGAGGATTAGGGACCGGTCTTATTTATAACTTAGGGGCAACTGGAGGAATGGCCGCACCTGTATTAGCTACATACATTTCAGGATATTATGGCTTAGGTGTTTCATTATTCATTGTTACGGTTGCATTCTCTGCCt                                                                                                                             >  1:412978/1‑149 (MQ=255)

                       tCCAACAAAATTAAGAGGATTAGGGACCGGTCTTATTTATAACTTAGGGGCAACTGGAGGAATGGCCGCACCTGTATTAGCTACATACATTTCAGGATATTATGGCTTAGGTGTTTCATTATTCATTGTTACGGTTGCATTCTCTGCCt                                                                                                                             >  1:1322575/1‑149 (MQ=255)

                       tCCAACAAAATTAAGAGGATTAGGGACCGGTCTTATTTATAACTTAGGGGCAACTGGAGGAATGGCCGCACCTGTATTAGCTACATACATTTCAGGATATTATGGCTTAGGTGTTTCATTATTCATTGTTACGGTTGCATTCTCTGCCt                                                                                                                             <  2:2072413/149‑1 (MQ=255)

                         caacaaAATTAAGAGGATTAGGGACCGGTCTTATTTATAACTTAGGGGCAACTGGAGGAATGGCCGCACCTGTATTAGCTACATACATTTCAGGATATTATGGCTTAGGTGTTTCATTATTCATTGTTACGGTTGCATTCTCTGCCtta                                                                                                                           >  1:2185489/1‑149 (MQ=255)

                          aacaaAATTAAGAGGATTAGGGACCGGTCTTATTTATAACTTAGGGGCAACTGGAGGAATGGCCGCACCTGTATTAGCTACATACATTTCAGGATATTATGGCTTAGGTGTTTCATTATTCATTGTTACGGTTGCATTCTCTGCCttat                                                                                                                          <  1:3509176/149‑1 (MQ=255)

                           acaaAATTAAGAGGATTAGGGACCGGTCTTATTTATAACTTAGGGGCAACTGGAGGAATGGCCGCACCTGTATTAGCTACATACATTTCAGGATATTATGGCTTAGGTGTTTCATTATTAATTGTTACGGTTGCATTCTCTGCCttatt                                                                                                                         >  2:3767310/1‑149 (MQ=255)

                            caaAATTAAGAGGATTAGGGACCGGTCTTATTTATAACTTAGGGGCAACTGGAGGAATGGCCGCACCTGTATTAGCTACATACATTTCAGGATATTATGGCTTAGGTGTTTCATTATTCATTGTTACGGTTGCATTCTCTGCCttatta                                                                                                                        >  1:195904/1‑149 (MQ=255)

                             aaaaTTAAGAGGATTAGGGACCGGTCTTATTTATAACTTAGGGGCAACTGGAGGAATGGCCGCACCTGTATTAGCTACATACATTTCAGGATATTATGGCTTAGGTGTTTCATTATTCATTGTTACGGTTGCATTCTCTGCCTTATTaa                                                                                                                       >  1:1022769/1‑149 (MQ=255)

                               aaTTAAGAGGATTAGGGACCGGTCTTATTTATAACTTAGGGGCAACTGGAGGAATGGCCGCACCTGTATTAGCTACATACATTTCAGGATATTATGGCTTAGGTGTTTCATTATTCATTGTTACGGTTGCATTCTCTGCCTTATTAAtt                                                                                                                     <  2:1579371/149‑1 (MQ=255)

                                aTTAAGAGGATTAGGGACCGGTCTTATTTATAACTTAGGGGCAACTGGAGGAATGGCCGCACCTGTATTAGCTACATACATTTCAGGATATTATGGCTTAGGTGTTTCATTATTCATTGTTACGGTTGCATTCTCTGCCTTATTAAttt                                                                                                                    <  1:1767382/149‑1 (MQ=255)

                                 ttAAGAGGATTAGGGACCGGTCTTATTTATAACTTAGGGGCAACTGGAGGAATGGCCGCACCTGTATTAGCTACATACATTTCAGGATATTATGGCTTAGGTGTTTCATTATTCATTGTTACGGTTGCATTCTCTGCCTTATTAAtttt                                                                                                                   <  1:3781664/149‑1 (MQ=255)

                                  tAAGAGGATTAGGGACCGGTCTTATTTATAACTTAGGGGCAACTGGAGGAATGGCCGCACCTGTATTAGCTACATACATTTCAGGATATTATGGCTTAGGTGTTTCATTATTCATTGTTACGGTTGCATTCTCTGCCTTATTAATTTTg                                                                                                                  <  2:2167117/149‑1 (MQ=255)

                                    agagGATTAGGGACCGGTCTTATTTATAACTTAGGGGCAACTGGAGGAATGGCCGCACCTGTATTAGCTACATACATTTCAGGATATTATGGCTTAGGTGTTTCATTATTCATTGTTACGGTTGCATTCTCTGCCTTATTAATTTTGtt                                                                                                                >  1:2214239/1‑149 (MQ=255)

                                       ggATTAGGGACCGGTCTTATTTATAACTTAGGGGCAACTGGAGGAATGGCCGCACCTGTATTAGCTACATACATTTCAGGATATTATGGCTTAGGTGTTTCATTATTCATTGTTACGGTTGCATTCTCTGCCTTATTAATTTTGTTAGt                                                                                                             <  2:4297185/149‑1 (MQ=255)

                                       ggATTAGGGACCGGTCTTATTTATAACTTAGGGGCAACTGGAGGAATGGCCGCACCTGTATTAGCTACATACATTTCAGGATATTATGGCTTAGGTGTTTCATTATTAATTGTTACGGTTGCATTCTCTGCCTTATTAATTTTGTTAGt                                                                                                             <  1:1588306/149‑1 (MQ=255)

                                            aGGGACCGGTCTTATTTATAACTTAGGGGCAACTGGAGGAATGGCCGCACCTGTATTAGCTACATACATTTCAGGATATTATGGCTTAGGTGTTTCATTATTCATTGTTACGGTTGCATTCTCTGCCTTATTAATTTTGTTAGTTGGtt                                                                                                        >  1:2227815/1‑149 (MQ=255)

                                            aGGGACCGGTCTTATTTATAACTTAGGGGCAACTGGAGGAATGGCCGCACCTGTATTAGCTACATACATTTCAGGATATTATGGCTTAGGTGTTTCATTATTCATTGTTACGGTTGCATTCTCTGCCTTATTAATTTTGTTAGTTGGtt                                                                                                        >  1:4297650/1‑149 (MQ=255)

                                            aGGGACCGGTCTTATTTATAACTTAGGGGCAACTGGAGGAATGGCCGCACCTGTATTAGCTACATACATTTCAGGATATTATGGCTTAGGTGTTTCATTATTCATTGTTACGGTTGCATTCTCTGCCTTATTAATTTTGTTAGTTGGtt                                                                                                        >  1:2979557/1‑149 (MQ=255)

                                             gggACCGGTCTTATTTATAACTTAGGGGCAACTGGAGGAATGGCCGCACCTGTATTAGCTACATACATTTCAGGATATTATGGCTTAGGTGTTTCATTATTCATTGTTACGGTTGCATTCTCTGCCTTATTAATTTTGTTAGTTGGttt                                                                                                       <  2:5076544/149‑1 (MQ=255)

                                             gggACCGGTCTTATTTATAACTTAGGGGCAACTGGAGGAATGGCCGCACCTGTATTAGCTACATACATTTCAGGATATTATGGCTTAGGTGTTTCATTATTCATTGTTACGGTTGCATTCTCTGCCTTATTAATTTTGTTAGTTGGttt                                                                                                       >  2:3528014/1‑149 (MQ=255)

                                              ggACCGGTCTTATTTATAACTTAGGGGCAACTGGAGGAATGGCCGCACCTGTATTAGCTACATACATTTCAGGATATTATGGCTTAGGTGTTTCATTATTCATTGTTACGGTTGCATTCTCTGCCTTATTAATTTTGTTAGTTGGtttt                                                                                                      >  1:4128969/1‑149 (MQ=255)

                                              ggACCGGTCTTATTTATAACTTAGGGGCAACTGGAGGAATGGCCGCACCTGTATTAGCTACATACATTTCAGGATATTATGGCTTAGGTGTTTCATTATTCATTGTTACGGTTGCATTCTCTGCCTTATTAATTTTGTTAGTTGGtttt                                                                                                      <  1:3295434/149‑1 (MQ=255)

                                                aCCGGTCTTATTTATAACTTAGGGGCAACTGGAGGAATGGCCGCACCTGTATTAGCTACATACATTTCAGGATATTATGGCTTAGGTGTTTCATTATTCATTGTTACGGTTGCATTCTCTGCCTTATTAATTTTGTTAGTTGGTTTTGa                                                                                                    >  1:2900915/1‑149 (MQ=255)

                                                aCCGGTCTTATTTATAACTTAGGGGCAACTGGAGGAATGGCCGCACCTGTATTAGCTACATACATTTCAGGATATTATGGCTTAGGTGTTTCATTATTCATTGTTACGGTTGCATTCTCTGCCTTATTAATTTTGTTAGTTGGTTTTGa                                                                                                    <  2:2999177/149‑1 (MQ=255)

                                                    gTCTTATTTATAACTTAGGGGCAACTGGAGGAATGGCCGCACCTGTATTAGCTACATACATTTCAGGATATTATGGCTTAGGTGTTTCATTATTCATTGTTACGGTTGCATTCTCTGCCTTATTAATTTTGTTAGTTGGTTTTGATAtt                                                                                                >  1:3182708/1‑149 (MQ=255)

                                                    gTCTTATTTATAACTTAGGGGCAACTGGAGGAATGGCCGCACCTGTATTAGCTACATACATTTCAGGATATTATGGCTTAGGTGTTTCATTATTCATTGTTACGGTTGCATTCTCTGCCTTATTAATTTTGTTAGTTGGTTTTGATAtt                                                                                                <  2:1749482/149‑1 (MQ=255)

                                                    gTCTTATTTATAACTTAGGGGCAACTGGAGGAATGGCCGCACCTGTATTAGCTACATACATTTCAGGATATTATGGCTTAGGTGTTTCATTATTAATTGTTACGGTTGCATTCTCTGCCTTATTAATTTTGTTAGTTGGTTTTGATAtt                                                                                                >  1:3460811/1‑149 (MQ=255)

                                                     tCTTATTTATAACTTAGGGGCAACTGGAGGAATGGCCGCACCTGTATTAGCTACATACATTTCAGGATATTATGGCTTAGGTGTTTCATTATTCATTGTTACGGTTGCATTCTCTGCCTTATTAATTTTGTTAGTTGGTTTTGATATTc                                                                                               <  2:1235489/149‑1 (MQ=255)

                                                      cctatttatAACTTAGGGGCAACTGGAGGAATGGCCGCACCTGTATTAGCTACATACATTTCAGGATATTATGGCTTAGGTGTTTCATTATTCATTGTTACGGTTGCATTCTCTGCCTTATTAATTTTGTTAGTTGGTTTTGATATTcc                                                                                              >  2:1111923/3‑149 (MQ=255)

                                                      cTTATTTATAACTTAGGGGCAACTGGAGGAATGGCCGCACCTGTATTAGCTACATACATTTCAGGATATTATGGCTTAGGTGTTTCATTATTCATTGTTACGGTTGCATTCTCTGCCTTATTAATTTTGTTAGTTGGTTTTGATATTcc                                                                                              <  1:4804195/149‑1 (MQ=255)

                                                       ttatttatAACTTAGGGGCAACTGGAGGAATGGCCGCACCTGTATTAGCTACATACATTTCAGGATATTATGGCTTAGGTGTTTCATTATTAATTGTTACGGTTGCATTCTCTGCCTTATTAATTTTGTTAGTTGGTTTTGATATTCCa                                                                                             <  1:3130050/149‑1 (MQ=255)

                                                        tatttatAACTTAGGGGCAACTGGAGGAATGGCCGCACCTGTATTAGCTACATACATTTCAGGATATTATGGCTTAGGTGTTTCATTATTCATTGTTACGGTTGCATTCTCTGCCTTATTAATTTTGTTAGTTGGTTTTGATATTCCAg                                                                                            >  2:2874224/1‑149 (MQ=255)

                                                         atttatAACTTAGGGGCAACTGGAGGAATGGCCGCACCTGTATTAGCTACATACATTTCAGGATATTATGGCTTAGGTGTTTCATTATTCATTGTTACGGTTGCATTCTCTGCCTTATTAATTTTGTTAGTTGGTTTTGATATTCCAgg                                                                                           <  1:5020665/149‑1 (MQ=255)

                                                               aaCTTAGGGGCAACTGGAGGAATGGCCGCACCTGTATTAGCTACATACATTTCAGGATATTATGGCTTAGGTGTTTCATTATTCATTGTTACGGTTGCATTCTCTGCCTTATTAATTTTGTTAGTTGGTTTTGATATTCCAGGTAAAAt                                                                                     <  2:1830909/149‑1 (MQ=255)

                                                                 cTTAGGGGCAACTGGAGGAATGGCCGCACCTGTATTAGCTACATACATTTCAGGATATTATGGCTTAGGTGTTTCATTATTCATTGTTACGGTTGCATTCTCTGCCTTATTAATTTTGTTAGTTGGTTTTGATATTCCAGGTAAAAttt                                                                                   >  2:1427832/1‑149 (MQ=255)

                                                                 cTTAGGGGCAACTGGAGGAATGGCCGCACCTGTATTAGCTACATACATTTCAGGATATTATGGCTTAGGTGTTTCATTATTAATTGTTACGGTTGCATTCTCTGCCTTATTAATTTTGTTAGTTGGTTTTGATATTCCAGGTAAAAttt                                                                                   >  2:4653246/1‑149 (MQ=255)

                                                                  ttAGGTGCAACTGGAGGAATGGCCGCACCTGTATTAGCTAAATACATTTCAGGATATTATGTCTTAGGTGTTTCATTATTCATTTTTACGGTTGCATTCTCTGCCTTATTAATTTTGTTAGTTGGTTTTGATATTCCAGGTAAAATTta                                                                                  <  2:1374346/149‑1 (MQ=255)

                                                                  ttAGGGGCAACTGGAGGAATGGCCGCACCTGTATTAGCTACATACATTTCAGGATATTATGGCTTAGGTGTTTCATTATTCATTGTTACGGTTGCATTCTCTGCCTTATTAATTTTGTTAGTTGGTTTTGATATTCCAGGTAAAATTta                                                                                  <  2:387371/149‑1 (MQ=255)

                                                                  ttAGGGGCAACTGGAGGAATGGCCGCACCTGTATTAGCTACATACATTTCAGGATATTATGGCTTAGGTGTTTCATTATTCATTGTTACGGTTGCATTCTCTGCCTTATTAATTTTGTTAGTTGGTTTTGATATTCCAGGTAAAATTta                                                                                  <  2:4090553/149‑1 (MQ=255)

                                                                   tAGGGGCAACTGGAGGAATGGCCGCACCTGTATTAGCTACATACATTTCAGGATATTATGGCTTAGGTGTTTCATTATTCATTGTTACGGTTGCATTCTCTGCCTTATTAATTTTGTTAGTTGGTTTTGATATTCCAGGTAAAATTtat                                                                                 <  2:3372538/149‑1 (MQ=255)

                                                                   tAGGGGCAACTGGAGGAATGGCCGCACCTGTATTAGCTACATACATTTCAGGATATTATGGCTTAGGTGTTTCATTATTCATTGTTACGGTTGCATTCTCTGCCTTATTAATTTTGTTAGTTGGTTTTGATATTCCAGGTAAAATTtat                                                                                 >  2:4218706/1‑149 (MQ=255)

                                                                    aGGGGCAACTGGAGGAATGGCCGCACCTGTATTAGCTACATACATTTCAGGATATTATGGCTTAGGTGTTTCATTATTCATTGTTACGGTTGCATTCTCTGCCTTATTAATTTTGTTAGTTGGTTTTGATATTCCAGGTAAAATTtata                                                                                <  2:4985412/149‑1 (MQ=255)

                                                                     ggggCAACTGGAGGAATGGCCGCACCTGTATTAGCTACATACATTTCAGGATATTATGGCTTAGGTGTTTCATTATTCATTGTTACGGTTGCATTCTCTGCCTTATTAATTTTGTTAGTTGGTTTTGATATTCCAGGTAAAATTTATaa                                                                               >  2:258936/1‑149 (MQ=255)

                                                                     ggggCAACTGGAGGAATGGCCGCACCTGTATTAGCTACATACATTTCAGGATATTATGGCTTAGGTGTTTCATTATTAATTGTTACGGTTGCATTCTCTGCCTTATTAATTTTGTTAGTTGGTTTTGATATTCCAGGTAAAATTTATaa                                                                               <  2:4035043/149‑1 (MQ=255)

                                                                      gggCAACTGGAGGAATGGCCGCACCTGTATTAGCTACATACATTTCAGGATATTATGGCTTAGGTGTTTCATTATTCATTGTTACGGTTGCATTCTCTGCCTTATTAATTTTGTTAGTTGGTTTTGATATTCCAGGTAAAATTTATaaa                                                                              <  1:4716248/149‑1 (MQ=255)

                                                                        gCAACTGGAGGAATGGCCGCACCTGTATTAGCTACATACATTTCAGGATATTATGGCTTAGGTGTTTCATTATTCATTGTTACGGTTGCATTCTCTGCCTTATTAATTTTGTTAGTTGGTTTTGATATTCCAGGTAAAATTTATAAACt                                                                            <  2:5144143/149‑1 (MQ=255)

                                                                         cAACTGGAGGAATGGCCGCACCTGTATTAGCTACATACATTTCAGGATATTATGGCTTAGGTGTTTCATTATTCATTGTTACGGTTGCATTCTCTGCCTTATTAATTTTGTTAGTTGGTTTTGATATTCCAGGTAAAATTTATAAACTa                                                                           <  2:3963860/149‑1 (MQ=255)

                                                                           aCTGGAGGAATGGCCGCACCTGTATTAGCTACATACATTTCAGGATATTCTGGCTTAGGTGTTTCATTATTCATTGTTACGGTTGCATTCTCTGCCTTATTAATTTTGTTAGTTGGTTTTGATATTCCAGGTAAAATTTATAAACTATc                                                                         >  2:1027059/1‑149 (MQ=255)

                                                                           aCTGGAGGAATGGCCGCACCTGTATTAGCTACATACATTTCAGGATATTATGGCTTAGGTGTTTCATTATTCATTGTTACGGTTGCATTCTCTGCCTTATTAATTTTGTTAGTTGGTTTTGATATTCCAGGTAAAATTTATAAACTATc                                                                         >  2:4798879/1‑149 (MQ=255)

                                                                               gaggaATGGCCGCACCTGTATTAGCTACATACATTTCAGGATATTATGGCTTAGGTGTTTCATTATTCATTGTTACGGTTGCATTCTCTGCCTTATTAATTTTGTTAGTTGGTTTTGATATTCCAGGTAAAATTTATAAACTATCCGTg                                                                     >  1:1482974/1‑149 (MQ=255)

                                                                                    aTGGCCGCACCTGTATTAGCTACATACATTTCAGGATATTATGGCTTAGGTGTTTCATTATTCATTGTTACGGTTGCATTCTCTGCCTTATTAATTTTGTTAGTTGGTTTTGATATTCCAGGTAAAATTTATAAACTATCCGTGGCTaa                                                                >  2:3487665/1‑149 (MQ=255)

                                                                                     tGGCCGCACCTGTATTAGCTACATACATTTCAGGATATTATGGCTTAGGTGTTTCATTATTCATTGTTACGGTTGCATTCTCTGCCTTATTAATTTTGTTAGTTGGTTTTGATATTCCAGGTAAAATTTATAAACTATCCGTGGCTaaa                                                               <  2:2673389/149‑1 (MQ=255)

                                                                                       gCCGCACCTGTATTAGCTACATACATTTCAGGATATTATGGCTTAGGTGTTTCATTATTCATTGTTACGGTTGCATTCTCTGCCTTATTAATTTTGTTAGTTGGTTTTGATATTCCAGGTAAAATTTATAAACTATCCGTGGCTAAATg                                                             >  2:4884781/1‑149 (MQ=255)

                                                                                         cGCACCTGTATTAGCTACATACATTTCAGGATATTATGGCTTAGGTGTTTCATTATTCATTGTTACGGTTGCATTCTCTGCCTTATTAATTTTGTTAGTTGGTTTTGATATTCCAGGTAAAATTTATAAACTATCCGTGGCTAAATGAt                                                           <  2:254463/149‑1 (MQ=255)

                                                                                         cGCACCTGTATTAGCTACATACATTTCAGGATATTATGGCTTAGGTGTTTCATTATTCATTGTTACGGTTGCATTCTCTGCCTTATTAATTTTGTTAGTTGGTTTTGATATTCCAGGTAAAATTTATAAACTATCCGTGGCTAAATGAt                                                           >  2:342922/1‑149 (MQ=255)

                                                                                          gCACCTGTATTAGCTACATACATTTCAGGATATTATGGCTTAGGTGTTTCATTATTCATTGTTACGGTTGCATTCTCTGCCTTATTAATTTTGTTAGTTGGTTTTGATATTCCAGGTAAAATTTATAAACTATCCGTGGCTAAATGAtt                                                          >  2:2223350/1‑149 (MQ=255)

                                                                                          gCACCTGTATTAGCTACATACATTTCAGGATATTATGGCTTAGGTGTTTCATTATTCATTGTTACGGTTGCATTCTCTGCCTTATTAATTTTGTTAGTTGGTTTTGATATTCCAGGTAAAATTTATAAACTATCCGTGGCTAAATGAtt                                                          <  2:2135910/149‑1 (MQ=255)

                                                                                          gCACCTGTATTAGCTACATACATTTCAGGATATTATGGCTTAGGTGTTTCATTATTCATTGTTACGGTTGCATTCTCTGCCTTATTAATTTTGTTAGTTGGTTTTGATATTCCAGGTAAAATTTATAAACTATCCGTGGCTAAATGAtt                                                          >  2:3209990/1‑149 (MQ=255)

                                                                                             ccTGTATTAGCTACATACATTTCAGGATATTATGGCTTAGGTGTTTCATTATTAATTGTTACGGTTGCATTCTCTGCCTTATTAATTTTGTTAGTTGGTTTTGATATTCCAGGTAAAATTTATAAACTATCCGTGGCTAAATGATTGGa                                                       >  2:3650981/1‑149 (MQ=255)

                                                                                                   ttAGCTACATACATTTCAGGATATTATGGCTTAGGTGTTTCATTATTAATTGTTACGGTTGCATTCTCTGCCTTATTAATTTTGTTAGTTGGTTTTGATATTCCAGGTAAAATTTATAAACTATCCGTGGCTAAATGATTGGAGGCttt                                                 <  2:2411494/149‑1 (MQ=255)

                                                                                                      gCTACATACATTTCAGGATATTATGGCTTAGGTGTTTCATTATTCATTGTTACGGTTGCATTCTCTGCCTTATTAATTTTGTTAGTTGGTTTTGATATTCCAGGTAAAATTTATAAACTATCCGTGGCTAAATGATTGGAGGCTTTATg                                              <  1:749391/149‑1 (MQ=255)

                                                                                                        tacatacaTTTCAGGATATTATGGCTTAGGTGTTTCATTATTCATTGTTACGGTTGCATTCTCTGCCTTATTAATTTTGTTAGTTGGTTTTGATATTCCAGGTAAAATTTATAAACTATCCGTGGCTAAATGATTGGAGGCTTTATGat                                            <  2:1747274/149‑1 (MQ=255)

                                                                                                        tacatacaTTTCAGGATATTATGGCTTAGGTGTTTCATTATTCATTGTTACGGTTGCATTCTCTGCCTTATTAATTTTGTTAGTTGGTTTTGATATTCCAGGTAAAATTTATAAACTATCCGTGGCTAAATGATTGGAGGCTTTATGat                                            >  2:432936/1‑149 (MQ=255)

                                                                                                         atatacatTTCAGGATATTATGGCTTAGGTGTTTCATTATTCATTGTTACGGTTGCATTCTCTGCCTTATTAATTTTGTTAGTTGGTTTTGATATTCCAGGTAAAATTTATAAACTATCCGTGGCTAAATGATTGGAGGCTTTATGatt                                           >  2:449944/3‑149 (MQ=255)

                                                                                                         acatacatTTCAGGATATTATGGCTTAGGTGTTTCATTATTCATTGTTACGGTTGCATTCTCTGCCTTATTAATTTTGTTAGTTGGTTTTGATATTCCAGGTAAAATTTATAAACTATCCGTGGCTAAATGATTGGAGGCTTTATGatt                                           >  2:450120/1‑149 (MQ=255)

                                                                                                          catacatTTCAGGATATTATGGCTTAGGTGTTTCATTATTCATTGTTACGGTTGCATTCTCTGCCTTATTAATTTTGTTAGTTGGTTTTGATATTCCAGGTAAAATTTATAAACTATCCGTGGCTAAATGATTGGAGGCTTTATGatta                                          >  1:2814861/1‑149 (MQ=255)

                                                                                                            tacatTTCAGGATATTATGGCTTAGGTGTTTCATTATTCATTGTTACGGTTGCATTCTCTGCCTTATTAATTTTGTTAGTTGGTTTTGATATTCCAGGTAAAATTTATAAACTATCCGTGGCTAAATGATTGGAGGCTTTATGattaat                                        >  2:1722481/1‑149 (MQ=255)

                                                                                                              catTTCAGGATATTATGGCTTAGGTGTTTCATTATTCATTGTTACGGTTGCATTCTCTGCCTTATTAATTTTGTTAGTTGGTTTTGATATTCCAGGTAAAATTTATAAACTATCCGTGGCTAAATGATTGGAGGCTTTATGattaatta                                      >  1:565303/1‑149 (MQ=255)

                                                                                                               atTTCAGGATATTATGGCTTAGGTGTTTCATTATTCATTGTTACGGTTGCATTCTCTGCCTTATTAATTTTGTTAGTTGGTTTTGATATTCCAGGTAAAATTTATAAACTATCCGTGGCTAAATGATTGGAGGCTTTATGATTAATTAt                                     <  2:4681947/149‑1 (MQ=255)

                                                                                                                tttCAGGATATTATGGCTTAGGTGTTTCATTATTCATTGTTACGGTTGCATTCTCTGCCTTATTAATTTTGTTAGTTGGTTTTGATATTCCAGGTAAAATTTATAAAATATCAGTGGCTAAATGATTGGAGGATTTATGATTAATTATg                                    >  2:2777946/1‑149 (MQ=255)

                                                                                                                tttCAGGATATTATGGCTTAGGTGTTTCATTATTAATTGTTACGGTTGCATTCTCTGCCTTATTAATTTTGTTAGTTGGTTTTGATATTCCAGGTAAAATTTATAAACTATCCGTGGCTAAATGATTGGAGGCTTTATGATTAATTATg                                    >  1:2425668/1‑149 (MQ=255)

                                                                                                                    aGGATATTATGGCTTAGGTGTTTCATTATTCATTGTTACGGTTGCATTCTCTGCCTTATTAATTTTGTTAGTTGGTTTTGATATTCCAGGTAAAATTTATAAACTATCCGTGTCTAAATGATTGGAGGCTTTATGATTAATTATGGCgt                                >  2:271713/1‑149 (MQ=255)

                                                                                                                    aGGATATTATGGCTTAGGTGTTTCATTATTCATTGTTACGGTTGCATTCTCTGCCTTATTAATTTTGTTAGTTGGTTTTGATATTCCAGGTAAAATTTATAAACTATCCGTGGCTAAATGATTGGAGGCTTTATGATTAATTATGGCgt                                >  2:2269465/1‑149 (MQ=255)

                                                                                                                           tatGGCTTAGGTGTTTCATTATTAATTGTTACGGTTGCATTCTCTGCCTTATTAATTTTGTTAGTTGGTTTTGATATTCCAGGTAAAATTTATAAACTATCCGTGGCTAAATGATTGGAGGCTTTATGATTAATTATGGCGTtgttggt                         >  2:72691/1‑149 (MQ=255)

                                                                                                                              ggCTTAGGTGTTTCATTATTCATTGTTACGGTTGCATTCTCTGCCTTATTAATTTTGTTAGTTGGTTTTGATATTCCAGGTAAAATTTATAAACTATCCGTGGCTAAATGATTGGAGGCTTTATGATTAATTATGGCGTtgttggtgtt                      <  1:342922/149‑1 (MQ=255)

                                                                                                                                  tAGGTGTTTCATTATTCATTGTTACGGTTGCATTCTCTGCCTTATTAATTTTGTTAGTTGGTTTTGATATTCCAGGTAAAATTTATAAACTATCCGTGGCTAAATGATTGGAGGCTTTATGATTAATTATGGCGTTGTTGGTGTTGGAt                  <  2:3071734/149‑1 (MQ=255)

                                                                                                                                       gtTTCATTATTCATTGTTACGGTTGCATTCTCTGCCTTATTAATTTTGTTAGTTGGTTTTGATATTCCAGGTAAAATTTATAAACTATCCGTGGCTAAATGATTGGAGGCTTTATGATTAATTATGGCGTTGTTGGTGTTGGATACttt             >  2:4584312/1‑149 (MQ=255)

                                                                                                                                            attattAATTGTTACGGTTGCATTCTCTGCCTTATTAATTTTGTTAGTTGGTTTTGATATTCCAGGTAAAATTTATAAACTATCCGTGGCTAAATGATTGGAGGCTTTATGATTAATTATGGCGTTGTTGGTGTTGGATACTTTGgcgc        <  1:2484453/149‑1 (MQ=255)

                                                                                                                                             ttattCATTGTTACGGTTGCATTCTCTGCCTTATTAATTTTGTTAGTTGGTTTTGATATTCCAGGTAAAATTTATAAACTATCCGTGGCTAAATGATTGGAGGCTTTATGATTAATTATGGCGTTGTTGGTGTTGGATACTTTGGCGCt       >  1:1630622/1‑149 (MQ=255)

                                                                                                                                              tattCATTGTTACGGTTGCATTCTCTGCCTTATTAATTTTGTTAGTTGGTTTTGATATTCCAGGTAAAATTTATAAACTATCCGTGGCTAAATGATTGGAGGCTTTATGATTAATTATGGCGTTGTTGGTGTTGGATACTTTGGCGCTg      <  2:4735455/149‑1 (MQ=255)

                                                                                                                                                 tCATTGTTACGGTTGCATTCTCTGCCTTATTAATTTTGTTAGTTGGTTTTGATATTCCAGGTAAAATTTATAAACTATCCGTGGCTAAATGATTGGAGGCTTTATGATTAATTATGGCGTTGTTGGTGTTGGATACTTTGGCGCTGAAt   <  2:1885328/149‑1 (MQ=255)

                                                                                                                                                 tCATTGTTACGGTTGCATTCTCTGCCTTATTAATTTTGTTAGTTGGTTTTGATATTCCAGGTAAAATTTATAAACTATCCGTGGCTAAATGATTGGAGGCTTTATGATTAATTATGGCGTTGTTGGTGTTGGATACTTTGGCGCTGAAt   <  1:1081503/149‑1 (MQ=255)

                                                                                                                                                 tCATTGTTACGGTTGCATTCTCTGCCTTATTAATTTTGTTAGTTGGTTTTGATATTCCAGGTAAAATTTATAAACTATCCGTGGCTAAATGATTGGAGGCTTTATGATTAATTATGGCGTTGTTGGTGTTGGATACTTTGGCGCTGAAt   >  1:5094356/1‑149 (MQ=255)

                                                                                                                                                  cATTGTTACGGTTGCATTCTCTGCCTTATTAATTTTGTTAGTTGGTTTTGATATTCCAGGTAAAATTTATAAACTATCCGTGGCTAAATGATTGGAGGCTTTATGATTAATTATGGCGTTGTTGGTGTTGGATACTTTGGCGCTGAAtt  <  2:1693534/149‑1 (MQ=255)

CCAAAATTTATATATGATTACTTTCCAACAAAATTAAGAGGATTAGGGACCGGTCTTATTTATAACTTAGGGGCAACTGGAGGAATGGCCGCACCTGTATTAGCTACATACATTTCAGGATATTATGGCTTAGGTGTTTCATTATTCATTGTTACGGTTGCATTCTCTGCCTTATTAATTTTGTTAGTTGGTTTTGATATTCCAGGTAAAATTTATAAACTATCCGTGGCTAAATGATTGGAGGCTTTATGATTAATTATGGCGTTGTTGGTGTTGGATACTTTGGCGCTGAATT  >  CP009273/4494856‑4495150

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`