Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,512,693 T→A 86.4% K407* (AAG→TAG)  yjhG ← putative dehydratase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,512,6930TA86.4% 887.3 / 119.0 286K407* (AAG→TAG) yjhGputative dehydratase
Reads supporting (aligned to +/- strand):  ref base T (23/16);  new base A (136/111);  total (159/127)
Fisher's exact test for biased strand distribution p-value = 7.30e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.32e-01

Only 100 of 286 total aligned reads displayed.

GGCGGTGGATTTGATCACCGAACCTTCTGGCGCAATATTGCCCACCGGGAAGGTGATAGTTGAGGTTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTTGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGTGCTGCCGGTAACCGTCATAACGTCTTCATGCAACAATCCGAGGCTGCGCAGATGCAACATGACTTCCGGCACACCACCTGCCATAAAGGCA  >  CP009273/4512547‑4512838
                                                                                                                                                  |                                                                                                                                                 
ggCGGTGGATTTGATCACCGAACCTTCTGGCGCAATATTGCCCACCGGGAAGGTGATAGTTGAGGTTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCATGATCGAGCAGGAGTTGCTTGa                                                                                                                                                 >  1:3495459/1‑149 (MQ=255)
   ggTGGATTTGATCACCGAACCTTCTGGCGCAATATTGCCCTCCGGGTAGGTGATAGTTGAGGTTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAAcc                                                                                                                                              <  1:3411115/149‑1 (MQ=255)
     tGGATTTGATCACCGAACCTTCTGGCGCAATATTGCCCACCGGGAAGGTGATAGTTGAGGTTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGc                                                                                                                                            <  2:109513/149‑1 (MQ=255)
      ggATTTGATCACCGAACCTTCTGGCGCAATATTGCCCACCGGGAAGGTGATAGTTGAGGTTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCt                                                                                                                                           <  1:1182055/149‑1 (MQ=255)
      ggATTTGATCACCGAACCTTCTGGCGCAATATTGCCCACCGGGAAGGTGATAGTTGAGGTTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCt                                                                                                                                           >  1:3642490/1‑149 (MQ=255)
      ggATTTGATCACCGAACCTTCTGGCGCAATATTGCCCACCGGGAAGGTGATAGTTGAGGTTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCt                                                                                                                                           >  1:61412/1‑149 (MQ=255)
        aTTTGATCACCGAACCTTCTGGCGCAATATTGCCCACCGGGAAGGTGATAGTTGAGGTTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTTGAACCGCTGa                                                                                                                                         >  1:1546358/1‑149 (MQ=255)
        aTTTGATCACCGAACCTTCTGGCGCAATATTGCCCACCGGGAAGGTGATAGTTGAGGTTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGATCACTTCGTCAGCGTGGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGa                                                                                                                                         >  2:316582/1‑149 (MQ=255)
         tttGATCACCGAACCTTCTGGCGCAATATTGCCCACCGGGAAGGTGATAGTTGAGGTTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGAc                                                                                                                                        <  2:187864/149‑1 (MQ=255)
            gATCACCGAACCTTCTGGCGCAATATTGCCCACCGGGAAGGTGATAGTTGAGGTTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGcc                                                                                                                                     <  2:2999212/149‑1 (MQ=255)
               cACCGAACCTTCTGGCGCAATATTGCCCACCGGGAAGGTGATAGTTGAGGTTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTTGAACCGCTGACGCCGtt                                                                                                                                  >  1:1396369/1‑149 (MQ=255)
               cACCGAACCTTCTGGCGCAATATTGCCCACCGGGAAGGTGATAGTTGAGGTTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGtt                                                                                                                                  >  2:2842760/1‑149 (MQ=255)
               cACCGAACCTTCTGGCGCAATATTGCCCACCGGGAAGGTGATAGTTGAGGTTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGtt                                                                                                                                  <  2:1777995/149‑1 (MQ=255)
               cACCGAACCTTCTGGCGCAATATTGCCCACCGGGAAGGTGATAGTTGAGGTTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGtt                                                                                                                                  <  2:1342124/149‑1 (MQ=255)
               cACCGAACCTTCTGGCGCAATATTGCCCACCGGGAAGGTGATAGTTGAGGTTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGtt                                                                                                                                  >  2:5077413/1‑149 (MQ=255)
                aCCGAACCTTCTGGCGCAATATTGCCCACCGGGAAGGTGATAGTTGAGGTTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTTGAACCGCTGACGCCGTTc                                                                                                                                 >  1:750359/1‑149 (MQ=255)
                     aCCTTCTGGCGCAATATTGCCCACCGGGAAGGTGATAGTTGAGGTTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGt                                                                                                                            <  1:3575756/149‑1 (MQ=255)
                     aCCTTCTGGCGCAATATTGCCCACCGGGAAGGTGATAGTTGAGGTTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGt                                                                                                                            >  2:867018/1‑149 (MQ=255)
                       cTTCTGGCGCAATATTGCCCACCGGGAAGGTGATAGTTGAGGTTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTTGAACCGCTGACGCCGTTCGGAGTGc                                                                                                                          >  2:1429852/1‑149 (MQ=255)
                        ttCTGGCGCAATATTGCCCACCGGGAAGGTGATAGTTGAGGTTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCt                                                                                                                         >  1:4809445/1‑149 (MQ=255)
                           tGGCGCAATATTGCCCACCGGGAAGGTGATAGTTGAGGTTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTccc                                                                                                                      <  2:776029/149‑1 (MQ=255)
                             gcgcAATATTGCCCACCGGGAAGGTGATAGTTGAGGTTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCccac                                                                                                                    >  2:2426203/1‑149 (MQ=255)
                               gcAATATTGCCCACCGGGAAGGTGATAGTTGAGGTTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCccacca                                                                                                                  >  2:1557059/1‑149 (MQ=255)
                                cAATATTGCCCACCGGGAAGGTGATAGTTGAGGTTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAg                                                                                                                 >  2:2470954/1‑149 (MQ=255)
                                 aaTATTGCCCACCGGGAAGGTGATAGTTGAGGTTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGt                                                                                                                >  2:469943/1‑149 (MQ=255)
                                  atatTGCCCACCGGGAAGGTGATAGTTGAGGTTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTc                                                                                                               >  2:4048501/1‑149 (MQ=255)
                                       gCCCACCGGGAAGGTGATAGTTGAGGTTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGt                                                                                                          >  1:3464740/1‑149 (MQ=255)
                                       gCCCACCGGGAAGGTGATAGTTGAGGTTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGt                                                                                                          >  1:2216213/1‑149 (MQ=255)
                                       gCCCACCGGGAAGGTGATAGTTGAGGTTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGt                                                                                                          <  2:3443814/149‑1 (MQ=255)
                                       gCCCACCGGGAAGGTGATAGTTGAGGTTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGt                                                                                                          >  2:1288553/1‑149 (MQ=255)
                                       gCCCACCGGGAAGGTGATAGTTGAGGTTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGt                                                                                                          <  1:3868551/149‑1 (MQ=255)
                                        cccACCGGGAAGGTGATAGTTGAGGTTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGtt                                                                                                         <  1:867018/149‑1 (MQ=255)
                                            ccGGGAAGGTGATAGTTGAGGTTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTtttct                                                                                                     <  1:4451162/149‑1 (MQ=255)
                                             cGGGAAGGTGATAGTTGAGGTTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTtttctt                                                                                                    <  2:5063502/149‑1 (MQ=255)
                                               ggAAGGTGATAGTTGAGGTTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTtttctttc                                                                                                  <  1:4832792/149‑1 (MQ=255)
                                               ggAAGGTGATAGTTGAGGTTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTtttctttc                                                                                                  <  1:3745621/149‑1 (MQ=255)
                                                gAAGGTGATAGTTGAGGTTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCa                                                                                                 <  1:2967188/149‑1 (MQ=255)
                                                gAAGGTGATAGTTGAGGTTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCa                                                                                                 >  1:5215211/1‑149 (MQ=255)
                                                gAAGGTGATAGTTGAGGTTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCa                                                                                                 <  1:2967135/149‑1 (MQ=255)
                                                      gATAGTTGAGGTTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGtgc                                                                                           <  2:3826327/149‑1 (MQ=255)
                                                       aTAGTTGAGGTTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGtgct                                                                                          <  1:2538490/149‑1 (MQ=255)
                                                       aTAGTTGAGGTTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGtgct                                                                                          <  2:3383890/149‑1 (MQ=255)
                                                        tAGTTGAGGTTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGtgctg                                                                                         >  1:5013613/1‑149 (MQ=255)
                                                        tAGTTGAGGTTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGtgctg                                                                                         >  1:2326248/1‑149 (MQ=255)
                                                           ttGAGGTTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTTGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGTGCTGCCg                                                                                      <  2:405781/149‑1 (MQ=255)
                                                           ttGAGGTTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGTGCTGCCg                                                                                      >  2:1680035/1‑149 (MQ=255)
                                                           ttGAGGTTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGTGCTGCCg                                                                                      >  1:2559346/1‑149 (MQ=255)
                                                              aGGTTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGTGCTGCCGGTa                                                                                   >  1:5086343/1‑149 (MQ=255)
                                                                   aaTCCGCGCGCTTTTGCTTGCTGCGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGTGCTGCCGGTAACCGt                                                                              >  2:2147275/1‑149 (MQ=255)
                                                                      ccGCGCGCTTTTGCTTGCTGCGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTTGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGTGCTGCCGGTAACCGTCAt                                                                           <  2:711661/149‑1 (MQ=255)
                                                                      ccGCGCGCTTTTGCTTGCTGCGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGTGCTGCCGGTAACCGTCAt                                                                           <  2:2536896/149‑1 (MQ=255)
                                                                       cgcgcgCTTTTGCTTGCTGCGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGTGCTGCCGGTAACCGTCATa                                                                          >  2:1445188/1‑149 (MQ=255)
                                                                          gcgcTTTTGCTTGCTGCGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGTGCTGCCGGTAACCGTCATAACg                                                                       >  2:2231264/1‑149 (MQ=255)
                                                                            gcTTTTGCTTGCTGCGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGTGCTGCCGGTAACCGTCATAACGTc                                                                     >  2:4599306/1‑149 (MQ=255)
                                                                             cTTTTGCTTGCTGCGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGTGCTGCCGGTAACCGTCATAACGTCt                                                                    >  1:2800101/1‑149 (MQ=255)
                                                                             cTTTTGCTTGCTGCGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGTGCTGCCGGTAACCGTCATAACGTCt                                                                    >  1:4976887/1‑149 (MQ=255)
                                                                             cTTTTGCTTGCTGCGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGTGCTGCCGGTAACCGTCATAACGTCt                                                                    >  1:3504503/1‑149 (MQ=255)
                                                                              ttttGCTTGCTGCGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGTGCTGCCGGTAACCGTCATAACGTCtt                                                                   <  1:3045160/149‑1 (MQ=255)
                                                                                 tgcttgctGCGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGTGCTGCCGGTAACCGTCATAACGTCTTCAt                                                                >  2:43688/1‑149 (MQ=255)
                                                                                  gcttgctGCGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGTGCTGCCGGTAACCGTCATAACGTCTTCATg                                                               >  2:3428895/1‑149 (MQ=255)
                                                                                     tgctgcGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGTGCTGCCGGTAACCGTCATAACGTCTTCATGcaa                                                            >  1:3774435/1‑149 (MQ=255)
                                                                                     tgctgcGGAGACATGATCACTTCGTCAGCGTGGATTTGGTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGTGCTGCCGGTAACCGTCATAACGTCTTCATGcaa                                                            <  2:3345622/149‑1 (MQ=255)
                                                                                        tgcGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGTGCTGCCGGTAACCGTCATAACGTCTTCATGcaacaa                                                         >  1:4517993/1‑149 (MQ=255)
                                                                                         gcGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGTGCTGCCGGTAACCGTCATAACGTCTTCATGCAACAAt                                                        <  2:1249269/149‑1 (MQ=255)
                                                                                           ggAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTTGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGTGCTGCCGGTAACCGTCATAACGTCTTCATGCAACAATcc                                                      >  2:200844/1‑149 (MQ=255)
                                                                                              gaCATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCGGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCCGCGTGCTGCCGGTAACCGTCATACCGTCTTCATGCAACAAGCCgcg                                                   <  2:2651040/149‑3 (MQ=255)
                                                                                                 aTGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTTGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGTGCTGCCGGTAACCGTCATAACGTCTTCATGCAACAATCCGAGGCt                                                >  2:507916/1‑149 (MQ=255)
                                                                                                 aTGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGTGCTGCCGGTAACCGTCATAACGTCTTCATGCAACAATCCGAGGCt                                                >  2:3900348/1‑149 (MQ=255)
                                                                                                 aTGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGTGCTGCCGGTAACCGTCATAACGTCTTCATGCAACAATCCGAGGCt                                                <  1:3698256/149‑1 (MQ=255)
                                                                                                   gATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGTGCTGCCGGTAACCGTCATAACGTCTTCATGCAACAATCCGAGGCTgc                                              >  2:1613849/1‑149 (MQ=255)
                                                                                                   gATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGTGCTGCCGGTAACCGTCATAACGTCTTCATGCAACAATCCGAGGCTgc                                              >  2:1931382/1‑149 (MQ=255)
                                                                                                      cACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGTGCTGCCGGTAACCGTCATAACGTCTTCATGCAACAATCCGAGGCTGCGCa                                           >  2:2486513/1‑149 (MQ=255)
                                                                                                         ttCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGTGCTGCCGGTAACCGTCATAACGTCTTCATGCAACAATCCGAGGCTGCGCAGAt                                        >  1:3281420/1‑149 (MQ=255)
                                                                                                          tCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGTGCTGCCGGTAACCGTCATAACGTCTTCATGCAACAATCCGAGGCTGCGCAGATg                                       >  1:5116069/1‑149 (MQ=255)
                                                                                                          tCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGTGCTGCCGGTAACCGTCATAACGTCTTCATGCAACAATCCGAGGCTGCGCAGATg                                       <  2:4128588/149‑1 (MQ=255)
                                                                                                           cGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGTGCTGCCGGTAACCGTCATAACGTCTTCATGCAACAATCCGAGGCTGCGCAGATGc                                      <  1:289751/149‑1 (MQ=255)
                                                                                                             tCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGTGCTGCCGGTAACCGTCATAACGTCTTCATGCAACAATCCGAGGCTGCGCAGATGCaa                                    <  2:4569948/149‑1 (MQ=255)
                                                                                                               aGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGTGCTGCCGGTAACCGTCATAACGTCTTCATGCAACAATCCGAGGCTGCGCAGATGCAACa                                  <  2:208138/149‑1 (MQ=255)
                                                                                                                gCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGTGCTGCCGGTAACCGTCATAACGTCTTCATGCAACAATCCGAGGCTGCGCAGATGCAACAt                                 >  2:338184/1‑149 (MQ=255)
                                                                                                                   ttGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGTGCTGCCGGTAACCGTCATAACGTCTTCATGCAACAATCCGAGGCTGCGCAGATGCAACATGAc                              >  2:854227/1‑149 (MQ=255)
                                                                                                                      aTTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGTGCTGCCGGTAACCGTCATAACGTCTTCATGCAACAATCCGAGGCTGCGCAGATGCAACATGACTTc                           >  2:3029463/1‑149 (MQ=255)
                                                                                                                       tttGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGTGCTGCCGGTAACCGTCATAACGTCTTCATGCAACAATCCGAGGCTGCGCAGATGCAACATGACTTcc                          >  1:3209898/1‑149 (MQ=255)
                                                                                                                       tttGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGTGCTGCCGGTAACCGTCATAACGTCTTCATGCAACAATCCGAGGCTGCGCAGATGCAACATGACTTcc                          <  2:2281582/149‑1 (MQ=255)
                                                                                                                       tttGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGTGCTGCCGGTAACCGTCATAACGTCTTCATGCAACAATCCGAGGCTGCGCAGATGCAACATGACTTcc                          <  1:1706777/149‑1 (MQ=255)
                                                                                                                          gTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGTGCTGCCGGTAACCGTCATAACGTCTTCATGCAACAATCCGAGGCTGCGCAGATGCAACATGACTTCCGGc                       <  1:111552/149‑1 (MQ=255)
                                                                                                                             ccTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGTGCTGCCGGTAACCGTCATAACGTCTTCATGCAACAATCCGAGGCTGCGCAGATGCAACATGACTTCCGGcaca                    >  2:3315620/1‑149 (MQ=255)
                                                                                                                               tGATCGAGCAGGAGTTGCTTGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGTGCTGCCGGTAACCGTCATAACGTCTTCATGCAACAATCCGAGGCTGCGCAGATGCAACATGACTTCCGGCAcacc                  >  1:4075038/1‑149 (MQ=255)
                                                                                                                               tGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGTGCTGCCGGTAACCGTCATAACGTCTTCATGCAACAATCCGAGGCTGCGCAGATGCAACATGACTTCCGGCAcacc                  >  1:4271770/1‑149 (MQ=255)
                                                                                                                               tGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGTGCTGCCGGTAACCGTCATAACGTCTTCATGCAACAATCCGAGGCTGCGCAGATGCAACATGACTTCCGGCAcacc                  <  1:212791/149‑1 (MQ=255)
                                                                                                                                 aTCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGTGCTGCCGGTAACCGTCATAACGTCTTCATGCAACAATCCGAGGCTGCGCAGATGCAACATGACTTCCGGCAcaccac                >  2:619436/1‑149 (MQ=255)
                                                                                                                                 aTCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGTGCTGCCGGTAACCGTCATAACGTCTTCATGCAACAATCCGAGGCTGCGCAGATGCAACATGACTTCCGGCAcaccac                >  1:4605583/1‑149 (MQ=255)
                                                                                                                                   cGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGTGCTGCCGGTAACCGTCATAACGTCTTCATGCAACAATCCGAGGCTGCGCAGATGCAACATGACTTCCGGCACACCACCt              >  2:941677/1‑149 (MQ=255)
                                                                                                                                   cGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGTGCTGCCGGTAACCGTCATAACGTCTTCATGCAACAATCCGAGGCTGCGCAGATGCAACATGACTTCCGGCACACCACCt              <  1:811568/149‑1 (MQ=255)
                                                                                                                                   cGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGTGCTGCCGGTAACCGTCATAACGTCTTCATGCAACAATCCGAGGCTGCGCAGATGCAACATGACTTCCGGCACACCACCt              <  2:1804463/149‑1 (MQ=255)
                                                                                                                                      gCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGTGCTGCCGGTAACCGTCATAACGTCTTCATGCAACAATCCGAGGCTGCGCAGATGCAACATGACTTCCGGCACACCACCTGcc           >  2:3690528/1‑149 (MQ=255)
                                                                                                                                       cAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGTGCTGCCGGTAACCGTCATAACGTCTTCATGCAACAATCCGAGGCTGCGCAGATGCAACATGACTTCCGGCACACCACCTGCCa          <  2:3504503/149‑1 (MQ=255)
                                                                                                                                        aGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGTGCTGCCGGTAACCGTCATAACGTCTTCATGCAACAATCCGAGGCTGCGCAGATGCAACATGACTTCCGGCACACCACCTGCCAt         >  2:257343/1‑149 (MQ=255)
                                                                                                                                              tGCTTGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGTGCTGCCGGTAACCGTCATAACGTCTTCATGCAACAATCCGAGGCTGCGCAGATGCAACATGACTTCCGGCACACCACCTGCCATAAAGGc   >  1:4738639/1‑149 (MQ=255)
                                                                                                                                              tGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGTGCTGCCGGTAACCGTCATAACGTCTTCATGCAACAATCCGAGGCTGCGCAGATGCAACATGACTTCCGGCACACCACCTGCCATAAAGGc   <  1:1245648/149‑1 (MQ=255)
                                                                                                                                               gCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGTGCTGCCGGTAACCGTCATAACGTCTTCATGCAACAATCCGAGGCTGCGCAGATGCAACATGACTTCCGGCACACCACCTGCCATAAAGGCa  <  1:2226497/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
GGCGGTGGATTTGATCACCGAACCTTCTGGCGCAATATTGCCCACCGGGAAGGTGATAGTTGAGGTTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTTGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGTGCTGCCGGTAACCGTCATAACGTCTTCATGCAACAATCCGAGGCTGCGCAGATGCAACATGACTTCCGGCACACCACCTGCCATAAAGGCA  >  CP009273/4512547‑4512838

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑