BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	4,546,084	G→A	5.8%	H18H (CAC→CAT)	yjiC ←	uncharacterized protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	4,546,084	0	G	A	5.8%	869.1 / 26.6	242	H18H (CAC→CAT)	yjiC	uncharacterized protein
Reads supporting (aligned to +/- strand): ref base G (82/146); new base A (10/4); total (92/150)
Fisher's exact test for biased strand distribution p-value = 1.07e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.30e-01

Only 100 of 256 total aligned reads displayed.

AAGTTTTCTAATTTCGACAACGCTTTTTCCATCGTAATTATGGTTATTGCCACGAGTAAGAGGCGGAAGATAGATGTTTTCCGAATCCATGCGTGCATATTTTAATTTACGTGTAAACTCACTGTCGACTATTGGGTGATGAAGGAAGTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCTTAATGGCATAGACATAATAGTTACCTTTTATGAGTATTTCACTGATGTTTAGAAAAATAGATAAATTTTTCTGTAGTAAAAAGAGAAGTAAACAAATGACATGCATGTTT  >  CP009273/4545937‑4546232

aaGTTTTCTAATTTCGACAACGCTTTTTCCATCGTAATTATGGTTATTGCCACGAGTAAGAGGCGGAAGATAGATGTTTTCCGAATCCATGCGTGCATATTTTAATTTACGTGTAAACTCACTGTCGACTATTGGGTGATGAAGGAAGt                                                                                                                                                     <  2:2172346/149‑1 (MQ=255)

 aGTTTTCTAATTTCGACAACGCTTTTTCCATCGTAATTATGGTTATTGCCACGAGTAAGAGGCGGAAGATAGATGTTTTCCGAATCCATGCGTGCATATTTTAATTTACGTGTAAACTCACTGTCGACTATTGGGTGATGAAGGAAGTg                                                                                                                                                    <  2:1545204/149‑1 (MQ=255)

    tttCTAATTTCGACAACGCTTTTTCCATCGTAATTATGGTTATTGCCACGAGTAAGAGGCGGAAGATAGATGTTTTCCGAATCCATGCGTGCATATTTTAATTTACGTGTAAACTCACTGTCGACTATTGGGTGATGAAGGAAGTGAGc                                                                                                                                                 <  1:4672607/149‑1 (MQ=255)

     ttCTAATTTCGACAACGCTTTTTCCATCGTAATTATGGTTATTGCCACGAGTAAGAGGCGGAAGATAGATGTTTTCCGAATCCATGCGTGCATATTTTAATTTACGTGTAAACTCACTGTCGACTATTGGGTGATGAAGGAAGTGAGCa                                                                                                                                                >  1:3080858/1‑149 (MQ=255)

     ttCTAATTTCGACAACGCTTTTTCCATCGTAATTATGGTTATTGCCACGAGTAAGAGGCGGAAGATAGATGTTTTCCGAATCCATGCGTGCATATTTTAATTTACGTGTAAACTCACTGTCGACTATTGGGTGATGAAGGAAGTGAGCa                                                                                                                                                >  1:802313/1‑149 (MQ=255)

       cTAATTTCGACAACGCTTTTTCCATCGTAATTATGGTTATTGCCACGAGTAAGAGGCGGAAGATAGATGTTTTCCGAATCCATGCGTGCATATTTTAATTTACGTGTAAACTCACTGTCGACTATTGGGTGATGAAGGAAGTGAGCATc                                                                                                                                              <  1:2012110/149‑1 (MQ=255)

           tttCGACAACGCTTTTTCCATCGTAATTATGGTTATTGCCACGAGTAAGAGGCGGAAGATAGATGTTTTCCGAATCCATGCGTGCATATTTTAATTTACGTGTAAACTCACTGTCGACTATTGGGTGATGAAGGAAGTGAGCATCTGta                                                                                                                                          <  1:791230/149‑1 (MQ=255)

                aCAACGCTTTTTCCATCGTAATTATGGTTATTGCCACGAGTAAGAGGCGGAAGATAGATGTTTTCCGAATCCATGCGTGCATATTTTAATTTACGTGTAAACTCACTGTCGACTATTGGGTGATGAAGGAAGTGAGCATCTGTaataat                                                                                                                                     >  1:1248833/1‑149 (MQ=255)

                 cAACGCTTTTTCCATCGTAATTATGGTTATTGCCACGAGTAAGAGGCGGAAGATAGATGTTTTCCGAATCCATGCGTGCATATTTTAATTTACGTGTAAACTCACTGTCGACTATTGGGTGATGAAGGAAGTGAGCATCTGTAATAAtt                                                                                                                                    <  2:2604881/149‑1 (MQ=255)

                   aCGCTTTTTCCATCGTAATTATGGTTATTGCCACGAGTAAGAGGCGGAAGATAGATGTTTTCCGAATCCATGCGTGCATATTTTAATTTACGTGTAAACTCACTGTCGACTATTGGGTGATGAAGGAAGTGAGCATCTGTAATAATTTg                                                                                                                                  <  1:3428575/149‑1 (MQ=255)

                      cTTTTTCCATCGTAATTATGGTTATTGCCACGAGTAAGAGGCGGAAGATAGATGTTTTCCGAATCCATGCGTGCATATTTTAATTTACGTGTAAACTCACTGTCGACTATTGGGTGATGAAGGAAATGAGCATCTGTAATAATTTGAct                                                                                                                               >  1:5090916/1‑149 (MQ=255)

                            ccATCGTAATTATGGTTATTGCCACGAGTAAGAGGCGGAAGATAGATGTTTTCCGAATCCATGCGTGCATATTTTAATTTACGTGTAAACTCACTGTCGACTATTGGGTGATGAAGGAAGTGAGCATCTGTAATAATTTGACTCTGAAg                                                                                                                         <  1:4669923/149‑1 (MQ=255)

                              aTCGTAATTATGGTTATTGCCACGAGTAAGAGGCGGAAGATAGATGTTTTCCGAATCCATGCGTGCATATTTTAATTTACGTGTAAACTCACTGTCGACTATTGGGTGATGAAGGAAGTGAGCATCTGTAATAATTTGACTCTGAAggg                                                                                                                       >  1:1226062/1‑149 (MQ=255)

                              aTCGTAATTATGGTTATTGCCACGAGTAAGAGGCGGAAGATAGATGTTTTCCGAATCCATGCGTGCATATTTTAATTTACGTGTAAACTCACTGTCGACTATTGGGTGATGAAGGAAGTGAGCATCTGTAATAATTTGACTCTGAAggg                                                                                                                       <  1:1770508/149‑1 (MQ=255)

                               tCGTAATTATGGTTATTGCCACGAGTAAGAGGCGGAAGATAGATGTTTTCCGAATCCATGCGTGCATATTTTAATTTACGTGTAAACTCACTGTCGACTATTGGGTGATGAAGGAAGTGAGCATCTGTAATAATTTGACTCTGAAGGGc                                                                                                                      <  1:2839373/149‑1 (MQ=255)

                               tCGTAATTATGGTTATTGCCACGAGTAAGAGGCGGAAGATAGATGTTTTCCGAATCCATGCGTGCATATTTTAATTTACGTGTAAACTCACTGTCGACTATTGGGTGATGAAGGAAGTGAGCATCTGTAATAATTTGACTCTGAAGGGc                                                                                                                      <  2:5133867/149‑1 (MQ=255)

                                cGTAATTATGGTTATTGCCACGAGTAAGAGGCGGAAGATAGATGTTGTCCGAATCCATGCGTGCATATTTTAATTTACGTGTAAACTCACTGTCGACTATTGGGTGATGAAGGAAGTGAGCATCTGTAATAATTTGACTCTGAAGGGCa                                                                                                                     <  1:1493054/149‑1 (MQ=255)

                                 gTAATTATGGTTATTGCCACGAGTAAGAGGCGGAAGATAGATGTTTTCCGAATCCATGCGTGCATATTTTAATTTACGTGTAAACTCACTGTCGACTATTGGGTGATGAAGGAAGTGAGCATCTGTAATAATTTGACTCTGAAGGGCAt                                                                                                                    <  1:1578111/149‑1 (MQ=255)

                                     ttATGGTTATTGCCACGAGTAAGAGGCGGAAGATAGATGTTTTCCGAATCCATGCGTGCATATTTTAATTTACGTGTAAACTCACTGTCGACTATTGGGTGATGAAGGAAGTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCt                                                                                                                >  2:2407533/1‑149 (MQ=255)

                                     ttATGGTTATTGCCACGAGTAAGAGGCGGAAGATAGATGTTTTCCGAATCCATGCGTGCATATTTTAATTTACGTGTAAACTCACTGTCGACTATTGGGTGATGAAGGAAGTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCt                                                                                                                <  2:3130605/149‑1 (MQ=255)

                                     ttATGGTTATTGCCACGAGTAAGAGGCGGAAGATAGATGTTTTCCGAATCCATGCGTGCATATTTTAATTTACGTGTAAACTCACTGTCGACTATTGGGTGATGAAGGAAGTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCt                                                                                                                >  1:4463054/1‑149 (MQ=255)

                                       aTGGTTATTGCCACGAGTAAGAGGCGGAAGATAGATGTTTTCCGAATCCATGCGTGCATATTTTAATTTACGTGTAAACTCACTGTCGACTATTGGGTGATGAAGGAAGTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCTTa                                                                                                              <  2:768342/149‑1 (MQ=255)

                                       aTGGTTAGTGCCACGAGTAAGAGGCGGAAGATAGATGTTTTCCGAATCCATGCGTGCATATTTTAATTTACGTGTAAACTCACTGTCGACTATTGGGTGATGAAGGAAGTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCTTa                                                                                                              <  2:4159106/149‑1 (MQ=255)

                                        tGGTTATTGCCACGAGTAAGAGGCGGAAGATAGATGTTTTCCGAATCCATGCGTGCATATTTTAATTTACGTGTAAACTCACTGTCGACTATTGGGTGATGAAGGAAGTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCTTaa                                                                                                             <  1:5001250/149‑1 (MQ=255)

                                         ggTTATTGCCACGAGTAAGAGGCGGAAGATAGATGTTTTCCGAATCCATGCGTGCATATTTTAATTTACGTGTAAACTCACTGTCGACTATTGGGTGATGAAGGAAGTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCTTAAt                                                                                                            <  2:2463085/149‑1 (MQ=255)

                                         ggTTATTGCCACGAGTAAGAGGCGGAAGATAGATGTTTTCCGAATCCATGCGTGCATATTTTAATTTACGTGTAAACTCACTGTCGACTATTGGGTGATGAAGGAAATGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCTTAAt                                                                                                            <  2:918873/149‑1 (MQ=255)

                                             aTTGCCACGAGTAAGAGGCGGAAGATAGATGTTTTCCGAATCCATGCGTGCATATTTTAATTTACGTGTAAACTCACTGTCGACTATTGGGTGATGAAGGAAGTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCTTAATGGCa                                                                                                        <  1:1855886/149‑1 (MQ=255)

                                               tGCCACGAGTAAGAGGCGGAAGATAGATGTTTTCCGAATCCATGCGTGCATATTTTAATTTACGTGTAAACTCACTGTCGACTATTGGGTGATGAAGGAAGTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCTTAATGGCATa                                                                                                      <  1:1338005/149‑1 (MQ=255)

                                                    cGAGTAAGAGGCGGAAGATAGATGTTTTCCGAATCCATGCGTGCATATTTTAATTTACGTGTAAACTCACTGTCGACTATTGGGTGATGAAGGAAGTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCTTAATGGCATAGACat                                                                                                 >  1:4227665/1‑149 (MQ=255)

                                                     gAGTAAGAGGCGGAAGATAGATGTTTTCCGAATCCATGCGTGCATATTTTAATTTACGTGTAAACTCACTGTCGACTATTGGGTGATGAAGGAAGTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCTTAATGGCATAGACata                                                                                                >  2:4470436/1‑149 (MQ=255)

                                                     gAGTAAGAGGCGGAAGATAGATGTTTTCCGAATCCATGCGTGCATATTTTAATTTACGTGTAAACTCACTGTCGACTATTGGGTGATGAAGGAAGTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCTTAATGGCATAGACata                                                                                                <  1:4347246/149‑1 (MQ=255)

                                                      aGTAAGAGGCGGAAGATAGATGTTTTCCGAATCCATGCGTGCATATTTTAATTTACGTGTAAACTCACTGTCGACTATTGGGTGATGAAGGAAGTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCTTAATGGCATAGACataa                                                                                               <  1:521526/149‑1 (MQ=255)

                                                      aGTAAGAGGCGGAAGATAGATGTTTTCCGAATCCATGCGTGCATATTTTAATTTACGTGTAAACTCACTGTCGACTATTGGGTGATGAAGGAAGTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCTTAATGGCATAGACataa                                                                                               <  1:3447818/149‑1 (MQ=255)

                                                      aGTAAGAGGCGGAAGATAGATGTTTTCCGAATCCATGCGTGCATATTTTAATTTACGTGTAAACTCACTGTCGACTATTGGGTGATGAAGGAAGTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCTTAATGGCATAGACataa                                                                                               >  1:3851314/1‑149 (MQ=255)

                                                        tAAGAGGCGGAAGATAGATGTTTTCCGAATCCATGCGTGCATATTTTAATTTACGTGTAAACTCACTGTCGACTATTGGGTGATGAAGGAAGTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCTTAATGGCATAGACataata                                                                                             <  2:2262911/149‑1 (MQ=255)

                                                         aaGAGGCGGAAGATAGATGTTTTCCGAATCCATGCGTGCATATTTTAATTTACGTGTAAACTCACTGTCGACTATTGGGTGATGAAGGAAGTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCTTAATGGCATAGACATAATAg                                                                                            >  2:386646/1‑149 (MQ=255)

                                                         aaGAGGCGGAAGATAGATGTTTTCCGAATCCATGCGTGCATATTTTAATTTACGTGTAAACTCACTGTCGACTATTGGGTGATGAAGGAAGTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCTTAATGGCATAGACATAATAg                                                                                            <  1:427147/149‑1 (MQ=255)

                                                            agGCGGAAGATAGATGTTTTCCGAATCCATGCGTGCATATTTTAATTTACGTGTAAACTCACTGTCGACTATTGGGTGATGAAGGAAGTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCTTAATGGCATAGACATAATAGTTa                                                                                         <  1:2678473/149‑1 (MQ=255)

                                                              gCGGAAGATAGATGTTTTCCGAATCCATGCGTGCATATTTTAATTTACGTGTAAACTCACTGTCGACTATTGGGTGATGAAGGAAGTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCTTAATGGCATAGACATAATAGTTAcc                                                                                       <  2:3635191/149‑1 (MQ=255)

                                                                  aaGATAGATGTTTTCCGAATCCATGCGTGCATATTTTAATTTACGTGTAAACTCACTGTCGACTATTGGGTGATGAAGGAAGTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCTTAATGGCATAGACATAATAGTTACCtttt                                                                                   <  2:302895/149‑1 (MQ=255)

                                                                   agatagatGTTTTCCGAATCCATGCGTGCATATTTTAATTTACGTGTAAACTCACTGTCGACTATTGGGTGATGAAGGAAGTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCTTAATGGCATAGACATAATAGTTACCTTTTa                                                                                  >  2:4684136/1‑149 (MQ=255)

                                                                   agatagatGTTTTCCGAATCCATGCGTGCATATTTTAATTTACGTGTAAACTCACTGTCGACTATTGGGTGATGAAGGAAATGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCTTAATGGCATAGACATAATAGTTACCTTTTa                                                                                  <  2:3335652/149‑1 (MQ=255)

                                                                    gatagatGTTTTCCGAATCCATGCGTGCATATTTTAATTTACGTGTAAACTCACTGTCGACTATTGGGTGATGAAGGAAGTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCTTAATGGCATAGACATAATAGTTACCTTTTAt                                                                                 >  1:1665954/1‑149 (MQ=255)

                                                                    gatagatGTTTTCCGAATCCATGCGTGCATATTTTAATTTACGTGTAAACTCACTGTCGACTATTGGGTGATGAAGGAAGTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCTTAATGGCATAGACATAATAGTTACCTTTTAt                                                                                 >  1:2143669/1‑149 (MQ=255)

                                                                         atGTTTTCCGAATCCATGCGTGCTTATTTTAATTTACTTGTAAACTCACTGTCGACTATTGGGTGATGAAGGAAGTGAGCATCTGTAATAATTTGACTCTGATGGGCATTGCTTAATGGCATAGACATAATAGTTACCTTTTATGAGTa                                                                            <  1:2719155/149‑1 (MQ=255)

                                                                         atGTTTTCCGAATCCATGCGTGCATATTTTAATTTACGTGTAAACTCACTGTCGACTATTGGGTGATGAAGGAAGTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCTTAATGGCATAGACATAATAGTTACCTTTTATGAGTa                                                                            <  2:1533375/149‑1 (MQ=255)

                                                                         atGTTTTCCGAATCCATGCGTGCATATTTTAATTTACGTGTAAACTCACTGTCGACTATTGGGTGATGAAGGAAGTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCTTAATGGCATAGACATAATAGTTACCTTTTATGAGTa                                                                            >  1:2334782/1‑149 (MQ=255)

                                                                               tCCGAATCCATGCGTGCATATTTTAATTTACGTGTAAACTCACTGTCGACTATTGGGTGATGAAGGAAGTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCTTAATGGCATAGACATAATAGTTACCTTTTATGAGTATTTCAc                                                                      >  2:1807640/1‑149 (MQ=255)

                                                                                 cGAATCCATGCGTGCATATTTTAATTTACGTGTAAACTCACTGTCGACTATTGGGTGATGAAGGAAATGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCTTAATGGCATAGACATAATAGTTACCTTTTATGAGTATTTCACTg                                                                    <  1:3584064/149‑1 (MQ=255)

                                                                                  gAATCCCTGCGTGCATATTTTAATTTCCGTGTAAACTCACTGTCGACTATTGGGTGATGAAGGAAGTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCTTAATGGCATAGACATAATAGTTACCTTTTATGAGTATTTCACTGa                                                                   <  2:4389455/149‑1 (MQ=255)

                                                                                  gAATCCATGCGTGCATATTTTAATTTACGTGTAAACTCACTGTCGACTATTGGGTGATGAAGGAAGTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCTTAATGGCATAGACATAATAGTTACCTTTTATGAGTATTTCACTGa                                                                   <  2:1462111/149‑1 (MQ=255)

                                                                                   aaTCCATGCGTGCATATTTTAATTTACGTGTAAACTCACTGTCGACTATTGGGTGGTGAAGGAAGTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCTTAATGGCATAGACATAATAGTTACCTTTTATGAGTATTTCACTGAt                                                                  <  1:4388609/149‑1 (MQ=255)

                                                                                   aaTCCATGCGTGCATATTTTAATTTACGTGTAAACTCACTGTCGACTATTGGGTGATGAAGGAAGTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCTTAATGGCATAGACATAATAGTTACCTTTTATGAGTATTTCACTGAt                                                                  <  2:4969903/149‑1 (MQ=255)

                                                                                    aTCCATGCGTGCATATTTTAATTTACGTGTAAACTCACTGTCGACTATTGGGTGATGAAGGAAGTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCTTAATGGCATAGACATAATAGTTACCTTTTATGAGTATTTCACTGATg                                                                 <  2:677362/149‑1 (MQ=255)

                                                                                    aTCCATGCGTGCATATTTTAATTTACGTGTAAACTCACTGTCGACTATTGGGTGATGAAGGAAGTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCTTAATGGCATAGACATAATAGTTACCTTTTATGAGTATTTCACTGATg                                                                 <  1:243685/149‑1 (MQ=255)

                                                                                     tCCATGCGTGCATATTTTAATTTACGTGTAAACTCACTGTCGACTATTGGGTGATGAAGGAAGTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCTTAATGGCATAGACATAATAGTTACCTTTTATGAGTATTTCACTGATGt                                                                <  1:4793994/149‑1 (MQ=255)

                                                                                     tCCATGCGTGCATATTTTAATTTACGTGTAAACTCACTGTCGACTATTGGGTGATGAAGGAAGTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCTTAATGGCATAGACATAATAGTTACCTTTTATGAGTATTTCACTGATGt                                                                >  1:1873168/1‑149 (MQ=255)

                                                                                        aTGCGTGCATATTTTAATTTACGTGTAAACTCACTGTCGACTATTGGGTGATGAAGGAAGTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCTTAATGGCATAGACATAATAGTTACCTTTTATGAGTATTTCACTGATGTTTa                                                             <  1:1619131/149‑1 (MQ=255)

                                                                                        aTGCGTGCATATTTTAATTTACGTGTAAACTCACTGTCGACTATTGGGTGATGAAGGAAGTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCTTAATGGCATAGACATAATAGTTACCTTTTATGAGTATTTCACTGATGTTTa                                                             <  1:3142726/149‑1 (MQ=255)

                                                                                        aTGCGTGCATATTTTAATTTACGTGTAAACTCACTGTCGACTATTGGGTGATGAAGGAAGTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCTTAATGGCATAGACATAATAGTTACCTTTTATGAGTATTTCACTGATGTTTa                                                             >  2:2580013/1‑149 (MQ=255)

                                                                                          gCGTGCATATTTTAATTTACGTGTAAACTCACTGTCGACTATTGGGTGATGAAGGAAGTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCTTAATGGCATAGACATAATAGTTACCTTTTATGAGTATTTCACTGATGTTTAGa                                                           <  1:3756322/149‑1 (MQ=255)

                                                                                             tGCATATTTTAATTTACGTGTAAACTCACTGTCGACTATTGGGTGATGAAGGAAGTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCTTAATGGCATAGACATAATAGTTACCTTTTATGAGTATTTCACTGATGTTTAGaaaa                                                        >  1:2229675/1‑149 (MQ=255)

                                                                                             tGCATATTTTAATTTACGTGTAAACTCACTGTCGACTATTGGGTGATGAAGGAAGTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCTTAATGGCATAGACATAATAGTTACCTTTTATGAGTATTTCACTGATGTTTAGaaaa                                                        >  1:2505598/1‑149 (MQ=255)

                                                                                             tGCATATTTTAATTTACGTGTAAACTCACTGTCGACTATTGGGTGATGAAGGAAGTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCTTAATGGCATAGACATAATAGTTACCTTTTATGAGTATTTCACTGATGTTTAGaaaa                                                        >  1:1055982/1‑149 (MQ=255)

                                                                                                atatTTTAATTTACGTGTAAACTCACTGTCGACTATTGGGTGATGAAGGAAGTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCTTAATGGCATAGACATAATAGTTACCTTTTATGAGTATTTCACTGATGTTTAGAAAAATa                                                     >  1:31513/1‑149 (MQ=255)

                                                                                                 tatTTTAATTTACGTGTAAACTCACTGTCGACTATTGGGTGATGAAGGAAGTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCTTAATGGCATAGACATAATAGTTACCTTTTATGAGTATTTCACTGATGTTTAGAAAAATAg                                                    <  1:882157/149‑1 (MQ=255)

                                                                                                    tttAATTTACGTGTAAACTCACTGTCGACTATTGGGTGATGAAGGAAGTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCTTAATGGCATAGACATAATAGTTACCTTTTATGAGTATTTCACTGATGTTTAGAAAAATAGATa                                                 <  1:4724538/149‑1 (MQ=255)

                                                                                                    tttAATTTACGTGTAAACTCACTGTCGACTATTGGGTGATGAAGGAAATGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCTTAATGGCATAGACATAATAGTTACCTTTTATGAGTATTTCACTGATGTTTAGAAAAATAGATa                                                 >  1:696587/1‑149 (MQ=255)

                                                                                                     ttAATTTACGTGTAAACTCACTGTCGACTATTGGGTGATGAAGGAAGTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCTTAATGGCATAGACATAATAGTTACCTTTTATGAGTATTTCACTGATGTTTAGAAAAATAGATaa                                                >  2:4045151/1‑149 (MQ=255)

                                                                                                       aaTTTACGTGTAAACTCACTGTCGACTATTGGGTGATGAAGGAAGTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCTTAATGGCATAGACATAATAGTTACCTTTTATGAGTATTTCACTGATGTTTAGAAAAATAGATAAAt                                              >  1:3976465/1‑149 (MQ=255)

                                                                                                       aaTTTACGTGTAAACTCACTGTCGACTATTGGGTGATGAAGGAAGTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCTTAATGGCATAGACATAATAGTTACCTTTTATGAGTATTTCACTGATGTTTAGAAAAATAGATAAAt                                              >  1:419296/1‑149 (MQ=255)

                                                                                                        aTTTACGTGTAAACTCACTGTCGACTATTGGGTGATGAAGGAAGTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCTTAATGGCATAGACATAATAGTTACCTTTTATGAGTATTTCACTGATGTTTAGAAAAATAGATAAAtt                                             <  2:1387632/149‑1 (MQ=255)

                                                                                                        aTTTACGTGTAAACTCACTGTCGACTATTGGGTGATGAAGGAAGTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCTTAATGGCATAGACATAATAGTTACCTTTTATGAGTATTTCACTGATGTTTAGAAAAATAGATAAAtt                                             <  2:3608259/149‑1 (MQ=255)

                                                                                                        aTTTACGTGTAAACTCACTGTCGACTATTGGGTGATGAAGGAAGTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCTTAATGGCATAGACATAATAGTTACCTTTTATGAGTATTTCACTGATGTTTAGAAAAATAGATAAAtt                                             >  1:442707/1‑149 (MQ=255)

                                                                                                        aTTTACGTGTAAACTCACTGTCGACTATTGGGTGATGAAGGAAGTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCTTAATGGCATAGACATAATAGTTACCTTTTATGAGTATTTCACTGATGTTTAGAAAAATAGATAAAtt                                             <  2:4224085/149‑1 (MQ=255)

                                                                                                        aTTTACGTGTAAACTCACTGTCGACTATTGGGTGATGAAGGAAGTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCTTAATGGCATAGACATAATAGTTACCTTTTATGAGTATTTCACTGATGTTTAGAAAAATAGATAAAtt                                             <  2:1607722/149‑1 (MQ=255)

                                                                                                         tttACGTGTAAACTCACTGTCGACTATTGGGTGATGAAGGAAGTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCTTAATGGCATAGACATAATAGTTACCTTTTATGAGTATTTCACTGATGTTTAGAAAAATAGATAAAttt                                            >  1:592944/1‑149 (MQ=255)

                                                                                                         tttACGTGTAAACTCACTGTCGACTATTGGGTGATGAAGGAAATGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCTTAATGGCATAGACATAATAGTTACCTTTTATGAGTATTTCACTGATGTTTAGAAAAATAGATAAAttt                                            >  1:2014690/1‑149 (MQ=255)

                                                                                                              gtgtAAACTCACTGTCGACTATTGGGTGATGAAGGAAGTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCTTAATGGCATAGACATAATAGTTACCTTTTATGAGTATTTCACTGATGTTTAGAAAAATAGATAAATTTTTCTg                                       >  1:4525904/1‑149 (MQ=255)

                                                                                                               tgtAAACTCACTGTCGACTATTGGGTGATGAAGGAAGTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCTTAATGGCATAGACATAATAGTTACCTTTTATGAGTATTTCACTGATGTTTAGAAAAATAGATAAATTTTTCTgt                                      <  2:1118570/149‑1 (MQ=255)

                                                                                                               tgtAAACTCACTGTCGACTATTGGGTGATGAAGGAAGTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCTTAATGGCATAGACATAATAGTTACCTTTTATGAGTATTTCACTGATGTTTAGAAAAATAGATAAATTTTTCTgt                                      <  2:3737282/149‑1 (MQ=255)

                                                                                                                gtAAACTCACTGTCGACTATTGGGTGATGAAGGAAGTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCTTAATGGCATAGACATAATAGTTACCTTTTATGAGTATTTCACTGATGTTTAGAAAAATAGATAAATTTTTCTgta                                     <  1:3334017/149‑1 (MQ=255)

                                                                                                                    aCTCACTGTCGACTATTGGGTGATGAAGGAAGTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCTTAATGGCATAGACATAATAGTTACCTTTTATGAGTATTTCACTGATGTTTAGAAAAATAGATAAATTTTTCTGTAGTaa                                 <  1:2295997/149‑1 (MQ=255)

                                                                                                                        aCTGTCGACTATTGGGTGATGAAGGAAGTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCTTAATGGCATAGACATAATAGTTACCTTTTATGAGTATTTCACTGATGTTTAGAAAAATAGATAAATTTTTCTGTAGTAAAAag                             >  2:2100158/1‑149 (MQ=255)

                                                                                                                        aCTGTCGACTATTGGGTGATGAAGGAAGTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCTTAATGGCATAGACATAATAGTTACCTTTTATGAGTATTTCACTGATGTTTAGAAAAATAGATAAATTTTTCTGTAGTAAAAag                             <  1:3801439/149‑1 (MQ=255)

                                                                                                                          tGTCGACTATTGGGTGATGAAGGAAGTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCTTAATGGCATAGACATAATAGTTACCTTTTATGAGTATTTCACTGATGTTTAGAAAAATAGATAAATTTTTCTGTAGTAAAAagag                           >  1:4913896/1‑149 (MQ=255)

                                                                                                                          tGTCGACTATTGGGTGATGAAGGAAGTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCTTAATGGCATAGACATAATAGTTACCTTTTATGAGTATTTCACTGATGTTTAGAAAAATAGATAAATTTTTCTGTAGTAAAAagag                           >  1:4736693/1‑149 (MQ=255)

                                                                                                                            tCGACTATTGGGTGATGAAGGAAGTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCTTAATGGCATAGACATAATAGTTACCTTTTATGAGTATTTCACTGATGTTTAGAAAAATAGATAAATTTTTCTGTAGTAAAAAGAGaa                         <  1:2580013/149‑1 (MQ=255)

                                                                                                                                cTATTGGGTGATGAAGGAAGTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCTTAATGGCATAGACATAATAGTTACCTTTTATGAGTATTTCACTGATGTTTAGAAAAATAGATAAATTTTTCTGTAGTAAAAAGAGAAGTaa                     >  2:1069345/1‑149 (MQ=255)

                                                                                                                                cTATTGGGTGATGAAGGAAGTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCTTAATGGCATAGACATAATAGTTACCTTTTATGAGTATTTCACTGATGTTTAGAAAAATAGATAAATTTTTCTGTAGTAAAAAGAGAAGTaa                     >  1:28776/1‑149 (MQ=255)

                                                                                                                                  aTTGGGTGATGAAGGAAGTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCTTAATGGCATAGACATAATAGTTACCTTTTATGAGTATTTCACTGATGTTTAGAAAAATAGATAAATTTTTCTGTAGTAAAAAGAGAAGTAAAc                   <  2:3080858/149‑1 (MQ=255)

                                                                                                                                      ggTGATGAAGGAAGTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCTTAATGGCATAGACATAATAGTTACCTTTTATGAGTATTTCACTGATGTTTAGAAAAATAGATAAATTTTTCTGTAGTAAAAAGAGAAGTAAACAAAt               <  2:2704830/149‑1 (MQ=255)

                                                                                                                                      ggTGATGAAGGAAGTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCTTAATGGCATAGACATAATAGTTACCTTTTATGAGTATTTCACTGATGTTTAGAAAAATAGATAAATTTTTCTGTAGTAAAAAGAGAAGTAAACAAAt               <  1:386646/149‑1 (MQ=255)

                                                                                                                                              aggaagTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCTTAATGGCATAGACATAATAGTTACCTTTTATGAGTATTTCACTGATGTTTAGAAAAATATATAAATTTTTCTGTAGTAAAAAGAGAAGTAAAAAAATGAaatgca       >  2:5209715/1‑149 (MQ=255)

                                                                                                                                              aggaagTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCTTAATGGCATAGACATAATAGTTACCTTTTATGAGTATTTCACTGATGTTTAGAAAAATAGATAAATTTTTCTGTAGTAAAAAGAGAAGTAAACAAATGAcatgca       <  1:2269351/149‑1 (MQ=255)

                                                                                                                                                gaagTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCTTAATGGCATAGACATAATAGTTACCTTTTATGAGTATTTCACTGATGTTTAGAAAAATAGATAAATTTTTCTGTAGTAAAAAGAGAAGTAAACAAATGAcatgcatg     <  2:1947859/149‑1 (MQ=255)

                                                                                                                                                 aagTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCTTAATGGCATAGACATAATAGTTACCTTTTATGAGTATTTCACTGATGTTTAGAAAAATAGATAAATTTTTCTGTAGTAAAAAGAGAAGTAAACAAATGACATGCATGt    <  1:2407533/149‑1 (MQ=255)

                                                                                                                                                  agTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCTTAATGGCATAGACATAATAGTTACCTTTTATGAGTATTTCACTGATGTTTAGAAAAATAGATAAATTTTTCTGTAGTAAAAAGAGAAGTAAACAAATGACATGCATGtt   >  1:982296/1‑149 (MQ=255)

                                                                                                                                                  agTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCTTAATGGCATAGACATAATAGTTACCTTTTATGAGTATTTCACTGATGTTTAGAAAAATAGATAAATTTTTCTGTAGTAAAAAGAGAAGTAAACAAATGACATGCATGtt   <  1:2898430/149‑1 (MQ=255)

                                                                                                                                                   gTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCTTAATGGCATAGACATAATAGTTACCTTTTATGAGTATTTCACTGATGTTTAGAAAAATAGATAAATTTTTCTGTAGTAAAAAGAGAAGTAAACAAATGACATGCATGttt  >  1:2773797/1‑149 (MQ=255)

AAGTTTTCTAATTTCGACAACGCTTTTTCCATCGTAATTATGGTTATTGCCACGAGTAAGAGGCGGAAGATAGATGTTTTCCGAATCCATGCGTGCATATTTTAATTTACGTGTAAACTCACTGTCGACTATTGGGTGATGAAGGAAGTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCTTAATGGCATAGACATAATAGTTACCTTTTATGAGTATTTCACTGATGTTTAGAAAAATAGATAAATTTTTCTGTAGTAAAAAGAGAAGTAAACAAATGACATGCATGTTT  >  CP009273/4545937‑4546232

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`