| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 360,181 | G→C | 7.0% | intergenic (‑115/+480) | lacZ ← / ← lacI | pseudogene, truncated/DNA‑binding transcriptional repressor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 360,181 | 0 | G | C | 7.0% | 1029.6 / 13.3 | 329 | intergenic (‑115/+480) | lacZ/lacI | pseudogene, truncated/DNA‑binding transcriptional repressor |
| Reads supporting (aligned to +/- strand): ref base G (95/206); new base C (16/11); total (111/218) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.32e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 351 total aligned reads displayed.
TACCATCCAGCGCCACCATCCAGTGCAGGAGCTCCGATGATAAGCTGTCAAACATGAGAATTCCGATCCCCAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTT‑CGCAACGTTCAAATCCGC‑TCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTGATGCCTGGGGAATTGGGAATTAATTCTTGAAGACGAAAGGGCCTCGTGATACGCCTA > CP009273/360033‑360329 | tACCATCCAGCGCCACCATCCAGTGCAGGAGCTCCGATGATAAGCTGTCAAACATGAGAATTCCGATCCCCAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTGGCGT‑CGCAACGTTCAAATCCGC‑GCCcg > 1:3751677/1‑149 (MQ=255) aaacaacagataaaacgaaaggcccagtctttcgactgagcctttcgttttatttgatgcctggggaattAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCGT‑CGCAACGTTAAAATCCGC‑TCCcgg < 1:2583058‑M1/79‑1 (MQ=255) ccATCCAGCGCCACCATCCCGTGCAGGAGCTCCGATGATAAGCTGTCAAACATGAGAATTCCGATCCCCAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTT‑CGCAACGTTCAAATCCGC‑TCCcggc < 2:3601596/149‑1 (MQ=255) ccATCCAGCGCCACCATCCAGTGCAGGAGCTCCGATGATAAGCTGTCAAACATGAGAATTCCGATCCCCAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTT‑CGCAACGTTCAAATCCGC‑TCCcggc > 1:3447169/1‑149 (MQ=255) aTCCAGCGCCACCATCCAGTGCCGGAGCTCCGATGATAAGCTGTCAAACATGAGAATTCCGATCCCCAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTGGCGT‑CGCAACGTTCAAAACCGC‑GCCccgcgg > 1:4308210/1‑149 (MQ=25) aacagataaaacgaaaggcccagtctttcgactgagcctttcgttttatttgatgcctggggaattAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCGCCGGGCCGTTGGGT‑CGCCACGTGCCAATACGC‑TCccggcggc < 2:2210156‑M1/83‑2 (MQ=255) ccAGCGCCACCATCCAGTGCAGGAGCTCCGATGATAAGCTGTCAAACATGAGAATTCCGATCCCCAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGGTGCTT‑CGCAACGTTCAAAGCCGC‑GCCCGGCGGAt > 1:225573/1‑149 (MQ=255) acagataaaacgaaaggcccagtctttcgactgagcctttcgttttatttgatgccgggggaattAATTCCTGGCAGTTGATGGCGGGCGTCCTGCGCGCCACCCTCCGGGCCGGTGCTG‑CGCAACGTGCACAGGCGC‑Tcccggcgggt < 2:2862181‑M1/84‑3 (MQ=255) ccgcgcCACCATCCTGTGCAGGAGCTCCGATGATACGCTGTCAAACATGAGAATTCCGAGCCCCAATGCCTGGCTGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTT‑CGCAACGTTCAAATCCGC‑TCCCGGCGGAtt < 1:2865555/147‑1 (MQ=21) ccccgcCACCAGCCCGTGAAGGAGCTCCGATGATAAGCTGTCAAACATGAGAATACCGACCCCCATTTCCTGGCTGTTTTTGGGGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTT‑CGCAACGTTCAAATCCGC‑TCCCGGCGGAtt < 1:2769780/146‑1 (MQ=21) cAGCGCCACCATCCAGTGCAGGAGCTCCGATGATAAGCTGTCAAACATGAGAATTCCGATCCCCAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTT‑CGCAACGTTCAAATCCGC‑TCCCGGCGGAtt < 2:4580174/149‑1 (MQ=255) gcgcCACCATCCAGTGCAGGAGCTCCGATGATAAGCTGTCAAACATGAGAATTCCGATCCCCAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTT‑CGCAACGTTCAAATCCGC‑TCCCGGCGGATTTg < 2:5061131/149‑1 (MQ=255) gataaaacgaaaggcccagtctttcgactgagcctttcgttttatttgatgcctggggaattAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGGGT‑CGCAACGTTAAAATCCCC‑ACCCCGCGGatttt < 1:2675662‑M1/87‑2 (MQ=255) agacaacgaacggcccagtctttcgactgagcctttcgttttatttgatgcctggggaattAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTT‑CGCAACGTTCAAATCCGC‑TCCCGGCGGATTTGt > 2:2590753‑M1/62‑149 (MQ=255) taaaacgaaaggcccagtctttcgactgagcctttcgttttatttgatgcctggggaattAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGGTT‑CGCAACGTTCAAATACGC‑TCCCGGCGGATTTgta < 2:3132111‑M1/89‑2 (MQ=255) ccaccaGCCCGGGCCGGAGCTCCGATTATAAGCTGAAAAACATGAGAAGTCCGAGCCCCACTTCCTGGCAGTTTTGGGGGGGCGTCCTCCCCGCCACCCTCCGGGCCGTTGCTT‑CGCAACGTTCAAATCCGC‑TCCCGGCGGATTTGTcc < 1:1455651/149‑1 (MQ=17) aaacgaaaggcccagtctttcgactgagcctttcgttttatttgatgcctggggaattAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTT‑CGCAACGTTCAAATCCGC‑TCCCGGCGGATTTGTCCt < 2:2137179‑M1/91‑1 (MQ=255) ccaTCCAGTGCAGGAGCTCCGATGATAAGGTGACAAACCTGCCAATTCCGATCCCCAATTCCTGGCATTTTTTGGGGGGCGTCCGGCCCGCCACCCTCCGGGCCGTTGCTT‑CGCAACGTTCAAATCCGC‑TCCCGGCGGATTTGTCCTAc < 1:4493418/149‑1 (MQ=18) acgaaaggcccagtctttcgactgagcctttcgttttatttgatgcctggggaattAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGGTT‑AGCAACGTTGAAAAAAGC‑TCCCGGCGGATTTGTCCTAc < 1:5136209‑M1/93‑1 (MQ=255) caTCCAGTGCAGGAGCTCCGATGATAAGCTGTCAAACATGAGAATTCCGATCCCCAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTT‑CGCAACGTTCAAATCCGC‑TCCCGGCGGATTTGTCCTACt > 2:2724770/1‑149 (MQ=255) gaacggcccagtctttcgactgagcctttcgttttatttgatgcctggggaattAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTT‑CGCAACGTTCAAATCCGC‑TCCCGGCGGATTTGTCCTACTc > 2:3724026‑M1/55‑149 (MQ=255) gaaaggcccagtctttcgactgagcctttcgttttatttgatgcctggggaattAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTT‑CGCAACGTTCAAAAACGC‑AACCGGCGGAtttgtccaacaa < 1:1585698‑M1/95‑6 (MQ=255) gaaaggcccagtctttcgactgagcctttcgttttatttgatgcctggggaattAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTGGCTT‑CGCAACGGTCAAATCCGC‑GCCCGGCGGCTTTGTCCGActg < 2:3990759‑M1/95‑2 (MQ=255) aaaggcccagtctttcgactgagcctttcgttttatttgatgcctggggaattAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCGT‑CGCAACGTTCAAATCCGC‑TCCCGGCGGATTTGTCctacaca < 1:839747‑M1/96‑4 (MQ=255) gcccagtctttcgactgagcctttcgttttatttgatgcctggggaattAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTT‑CGCAACGTTCAAATCCGC‑TCCCGGCGGTTTTGTCCTACTCAGgag > 1:1043970‑M1/50‑149 (MQ=255) cccagtctttcgactgagcctttcttattattggatgcctggggaattAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTT‑CGCAACGTTCAAATCCGC‑TCCCGGCGGATTTGTCCTACTCAGgaga > 1:1114263‑M1/49‑149 (MQ=255) cccagtctttcgactgagcctttcgttttatttgatgcctggggaattAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTT‑CGCAACGTTCAAATCCGC‑TCCCGGCGGATTTGTCCTACTCAGgaga < 2:2932817‑M1/101‑1 (MQ=255) cccagtctttcgactgagcctttcgttttatttgatgcctggggaattAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTT‑CGCAAAGTTCAAATACGC‑ACCCGGCGGATTTTTGCTACTCAGgaga < 1:2533904‑M1/101‑1 (MQ=255) gCAGGAGCTCCTATTATAAGCTGTCAAACATTAGATTTCCGATCCCCAATTCCTGGCTTTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTT‑CGCAACGTTCAAATCCGC‑TCCCGGCGGATTTGTCCTACTCAGGagag < 1:1976849/149‑1 (MQ=18) tttcgactgagcctttcgttttatttgatgcctggggaattAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTT‑CGCAACGTTCAAATCCGC‑TCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCa < 2:280929‑M1/108‑1 (MQ=255) gCTCCGATGATATGCTGTCAAACATGAGAATTCCGATCCCCAATTCCTGGCTGTTTTGGGGGGGCGTCCGGCCCGCCACCCTCCGGGCCGTTGCTT‑CGCAACGTTCAAATCCGC‑TCCCGGCGGATTTGTCCTACTAAGGAGAGCGTTCa < 1:4908785/149‑1 (MQ=18) gCTCCGATGATAAGCTGTCAAACATGAGAATTCCGATCCCCAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTT‑CGCAACGTTCAAATCCGC‑TCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCa < 2:2780923/149‑1 (MQ=255) tcgactgagcctttcgttttatttgatgcctggggaattAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTT‑CGCAACGTTCAAATCCGC‑TCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCAcc < 2:1531229‑M1/110‑1 (MQ=255) tCCGATGATAAGCTGTCAAACATGAGAATTCCGATCCCCAAGTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTT‑CGCAACGTTCAAATCCGC‑TCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCAcc < 1:1308176/149‑1 (MQ=255) acctatgatatgatGTCAAACTTTTCAATTCCTATCCCCAATTCCTGGATGTTTATGGCGGGCTACCTGCCCCCCACCCTCCGGGCCGTTGCTT‑CGCAACGTTCAAATCCGC‑TCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCAcc < 1:948879/145‑1 (MQ=14) cgactgagcctttcgttttatttgatgcctggggaattAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTT‑CGCAACGTTCAAATCCGC‑TCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCg < 2:1258547‑M1/111‑1 (MQ=255) ctgagcctttcgttttatttgatgcctggggaattAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTT‑CGCAACGTTCAAATCCGC‑TCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGaca < 2:3179548‑M1/114‑1 (MQ=255) ctgagcctttcgttttatttgatgcctggggaattAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTT‑CGCAACGTTCAAATACGC‑AACCGGCGGATTTGTCCTACTCAGGAGAGCGTTAACCGaca < 1:1244391‑M1/114‑1 (MQ=255) ctgagcctttcgttttatttgatgcctggggaattAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCGT‑AGCCACGTTCAAATACGC‑TCCCGGCGGGTTTTTGCTAAACAGGAGGGCGTTCACCGaca < 1:4258516‑M1/114‑1 (MQ=255) gatAAGCTGTCAAACATGAGAATTCCGATCCCCAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTT‑CGCAACGTTCAAATCCGC‑TCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGacaaa < 2:1180146/149‑1 (MQ=255) gatAAGCTGTCAAACATGAGAATTCCGATCCCCAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTT‑AGCAACGTTCAAATACGC‑TCCCGGCGGATTGGTCCGACTCAGGAGAGCGTTCACCGaccaa > 1:4647458/1‑149 (MQ=18) gagcctttcgttttatttgatgcctggggaattAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTT‑CGCAACGTTCAAATCCGC‑ACCCGGCGGATTTGTCCTACACAGGAGAGCGTTCACCGacaaa < 2:4429714‑M1/116‑1 (MQ=255) gcctttcgttttatttgatgcctggggaattAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCGT‑CGCAACGTTCAAATCCGC‑ACCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGccaaaca < 2:231949‑M1/118‑1 (MQ=255) tttcgttttatttgatgcctggggaattAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTT‑CGCAACGTTCAAATCCGC‑TCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAacaaca < 2:2392814‑M1/121‑1 (MQ=255) tttcgttttatttgatgcctggggaattAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTT‑CGCAAAGTTCAAATCCCC‑TCCCCGCGGATTTGTCCGAATAACGAGAGCGTTCACCGACAAacaaca < 2:3572979‑M1/121‑1 (MQ=255) tttcgttttatttgatgcctggggaattAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTT‑AGCCACGTTCAAAAACCC‑TCCCCGCCGATTTGTACAAATCAGGATAGCCTTAAACGACAAacaaca < 1:1640768‑M1/121‑1 (MQ=255) tGTCAAACATGAGAATTCCGATCCCCAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTT‑CGCAACGTTCAAATCCGC‑TCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGa < 2:720114/149‑1 (MQ=255) aaaCATGAGAATTCCGATCCCCAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTT‑CGCCACGTTCAAATACGC‑ACCCGGCGGATTTTTCCTAAACAGGTGAGGCTTGACCGACAaacaaccccaaaa > 1:2872334/1‑142 (MQ=17) aaaCATGAGAATTCCGATCCCCAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTT‑CGCAACGTTCAAATCCGC‑TCCCCGCGGATTTGTCCTACACAGGAGAGCGTTCACCGACAAACAACAGAaaaa > 1:247658/1‑149 (MQ=255) ttatttgatgcctgggtaattAATTCCGGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTT‑CGCAACGTTCAAATCCGC‑TCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATaaaa > 1:3233249‑M1/22‑149 (MQ=255) ttatttgatgcctggggacttAATTCCTGTCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTT‑CGCAACGTTCAAATCCGC‑TCCCGGCGGATTTGTCCAACTAAGGCGAGCGTTCACCGACAAACAAAAGATaaaa < 2:1472563‑M1/128‑1 (MQ=255) ttatttgatgcctggggaattAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTT‑CGCAACGTTCAAATCCGC‑TCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATaaaa > 1:2597153‑M1/22‑149 (MQ=255) aaCATGAGAATTCCGATCCCCAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGGTT‑GGCAAACGTCAAAAACCC‑GACCGGCGGAATTGTCCAACACAGGAGAGCCGTCACCCACAAACAAAACATaaaa > 1:976421/1‑149 (MQ=17) tatttgatgcctggggaattAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTT‑CGCAACGTTCAAATCCGC‑TCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAAc < 2:208735‑M1/129‑1 (MQ=255) tGAGAATTCCGATCCCCAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGGGT‑CGCAAACGTGAAATACGC‑ACCCGGCGGGGTGTGCCGACGCCGGAGAGCGGGGCCCGACAAACACCACATAAAAccta > 1:2342080/1‑146 (MQ=9) tGAGAATTCCGATCCCCAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTT‑CGCAACGTTCAAATCCGC‑TCCCGGCGGATTTGTCCGACTCAGGAGGGCGTTCACCGACAAACCACAGATAAAACGaa > 2:4995999/1‑149 (MQ=255) tGAGAATTCCGATCCCCAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTT‑CGCAACGGTCAAAAACGCACCCCGGCGAATTT‑TCCCAAAAAGGTTAGCGTTCCACGACCAACAACAAATAAAAAGaa > 1:5040027/1‑149 (MQ=9) tGAGAATTCCGATCCCCAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCGT‑CGCAACGTTCAAATCCGC‑TCCCGGCGGATTTGTCCTACTCAGGAGCGCGTTCACCGACAAACAACCGATAAATCGac > 2:3857650/1‑148 (MQ=255) gagaATTCCGATCCCCAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCGT‑CGCAACGTTCCAATCCGC‑GCCCGGCGGAGTTGTCCGACGCAGGAGAGCGGTCACCGACACACCACAGAGAAAACGaaa > 2:1924048/1‑149 (MQ=14) tgcctggggaattAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGGTT‑CGCAACGGTCAAAACCGC‑TCCCCGCGGATTTGTCCTACTCAGGAGAGCGGTCACCCACCAACAAAAGATAAAAACAAAGGc < 1:1917218‑M1/136‑1 (MQ=255) tgcctggggaattAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTT‑CGCAAAGTTCAAAAACGC‑TACCCGCGGATTTGTACAACACAGGAGAGCGGTCACCGACAAACAACACATAAAaagaaatgc < 2:1344470‑M1/136‑4 (MQ=255) aaTTCCGATCCCCAATTCCTGGCTGTTTTTGGGGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTT‑CGCAACGTTCAAATCCGC‑TCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGc < 1:4190157/149‑1 (MQ=255) aTTCCGATCCCATTTTCTTGGCTGTTTTTGGCGGGCTTCCTGCCCGCCACCCTCCGGGCCGTTGCTT‑CGAAACGTTCAAATCCGC‑TCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGcc < 1:4064639/149‑1 (MQ=11) ttCCGATCCCCAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTT‑CGCAACGTTCAAATCCGC‑TCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGccc < 2:871378/149‑1 (MQ=255) ctggggaattAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTT‑CGCAACGTTCAAATCCGC‑TCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACACACAACAGATAAAACGAAAGGCCCa < 2:2541358‑M1/139‑1 (MQ=255) cGATCCCCAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGGTTGCCCAACG‑TCAAATCCGC‑CCCCCGCCGATTTTTCCAAATCAGGAGTGCGGTACCCGACAAACAAAAGATAAAAAGAAAAGcaccct > 1:4124241/1‑144 (MQ=9) cGATCCCCAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTT‑CGCAACGTTCAAATACGC‑ACCCCGCGGCTTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAAAAGATAAAACGAAAGGCCCAga > 1:3857485/1‑148 (MQ=14) gggaattAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTT‑CGCAACGTTCAAATCCGC‑TCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTc < 2:4806325‑M1/142‑1 (MQ=255) gggaattAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTT‑CGCAACGTTCAAATCCGC‑TCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTc < 2:4537264‑M1/142‑1 (MQ=255) gtaattATTTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTT‑CGCAACGTTCAAATCCGC‑TCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCt > 1:4995999‑M1/7‑149 (MQ=255) gaattAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTT‑CGCAACGTTCAAATCCGC‑TCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCtt > 1:3056433‑M1/6‑149 (MQ=255) aattAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCGT‑CGCAACGTTCAAATCCGC‑TCCCGGCGGGGTTGTCCTACTCAGGAGGGCGGTCACCGACAAACAACAGATAAAAAGAAAGGCCCCGTCttt < 2:492666‑M1/145‑1 (MQ=255) cccAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTT‑CGCAACGTTCAAATCCGC‑TCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTc < 2:3342923/149‑1 (MQ=35) attAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTT‑CGCAACGTTCAAATCCGC‑TCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCAACAAACAACAGATAAAACGa < 2:2966302‑M1/131‑1 (MQ=255) attAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTT‑CGCAACGGTCAAATACGC‑TCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAAAGAaaggcccactcgtta < 2:2303102‑M1/146‑8 (MQ=255) ttAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTT‑CGCAACGTTCAAATCCGC‑TCCCGGCGGATTTGTCCTACTCAGGAGAGCGtt < 2:5202985‑M1/106‑1 (MQ=255) ttAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTT‑CGCAACGTTCAAATCCGC‑TCCCGGCGGATTTGTCCTACTCAGGAGAGCGtt > 1:5203119‑M1/3‑108 (MQ=255) cacttCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTT‑CGCAACGTTCAAAGCCGC‑TCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGa < 1:3700865/146‑1 (MQ=14) aaTTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTT‑CGCAACGTTCAAATCCGC‑TCCCGGCGGATTTGTCCTACTCAGGAGAGCGt > 2:3909470/1‑105 (MQ=33) aaTTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTT‑CGCAACGTTCAAATCCGC‑TCCCGGCGGATTTGTCCTACTCAGGAGAGCGt < 1:3909470/105‑1 (MQ=33) aaTTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTT‑CGCAACGTTCAAATCCGC‑TCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGAc < 2:4455340/149‑1 (MQ=32) aTTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTT‑CGCAACGTTCAAATCCGC‑TCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACCAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACt > 2:4744193/1‑149 (MQ=17) aTTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTT‑CGCAACGTTCAAATCCGC‑TCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACt < 2:3420501/149‑1 (MQ=32) ttCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTT‑CGCAACGTTCAAATCCGC‑TCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTg < 1:3470223/149‑1 (MQ=31) tCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTT‑CGCAACGTTCAAATCCGC‑TCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGa < 2:5097607/149‑1 (MQ=11) ggggggcgGCCGGCCCGCCACCCTCCGGGCCGTTGCTT‑CGCAACGTTCAAATCCGC‑TCCCGGCGGATTTGTCCTACTCCGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTAt < 1:468216/146‑1 (MQ=0) cccGCCACCCTCCGGGCCGTTGCGT‑CGCAACGTTCAAATCCGC‑TCCCGGCGGATTTGTCCGACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCGTTCGACTGAGCCGTTCGGGTTATTTGATggcgggca > 2:565593/1‑142 (MQ=11) cACCCTCCGGGCCGTTGCTT‑CGCAACGTTCAAATCCGC‑TCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTGATGCCTGgggaattg < 1:3325508/149‑1 (MQ=35) aCCCTCCGGGCCGTTGCTT‑CGCAACGTTCAAATCCGC‑TCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTGATGCCTGgggaattgg > 2:788004/1‑149 (MQ=35) gCCGTTGCTT‑CGCAACGTTCAACTCCGC‑TCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTGATGCCTGGGGAATTGGGaattaatt < 2:3525488/149‑1 (MQ=255) cGTTGCTT‑CGCAACGTTCAAATCCGC‑TCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCAGCGACAAACAACAGATAAAACGAAAGGCCCAGTCGTGCGACTGAGCCTTTCGTTTTATTTGATGCCGGGGGAAGTGGGacttattgct > 2:1963661/1‑144 (MQ=11) cGTTGCTT‑CGCAACGTTCAAATCCGC‑TCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTGATGCCTGGGGAATTGGGAATTAATTCt < 1:4756391/149‑1 (MQ=255) gCTT‑CGCAACGTTCAAATCCGC‑TCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCGTTCGACTGAGCCTTTCGTTTTATTTGATGCCTGGGGAATTGGGAATTAATTCTTGaa > 2:2706713/1‑149 (MQ=255) tt‑CGCAACGTTCAAATCCGC‑TCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTGATGCCTGGGGAATTGGGAATTAATTCTTGAAGa < 1:3008585/149‑1 (MQ=255) t‑CGCAACGTTCAAATCCGC‑TCCCGGCGGATTTGTCCTACTCCGGAGAGCGTTCACCGACAAACAACCGATAAAACGACAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTGATGCCTGGGGAATTGGGAATTAATTCTTGAAGAc < 1:1525242/149‑1 (MQ=255) cGCAACGTTCAAATCCGC‑TCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTGATGCCTGGGGAATTGGGAATTAATTCTTGAAGACg < 2:1640768/149‑1 (MQ=255) aTCCGC‑TCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTGATGCCTgggg < 2:2817586/112‑1 (MQ=31) tCCGC‑TCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTGATGCCTGGGGAATTGGGAATTAATTCTTGAAGACGAAAGGGCCTCGTg < 1:2098084/149‑1 (MQ=255) gC‑TCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTGATGCCTGGGGAATTGGGAATTAATTCTTGAAGACGAAAGGGCCTCGTGATa < 1:2137179/149‑1 (MQ=255) gC‑TCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTGCGACTGAGCCTTTCGTTTTATTTGATGCCTGGGGAATTGGGAATTAATTCTTGACGACGACAGGGCCACGTGATa > 2:4321424/1‑149 (MQ=255) | TACCATCCAGCGCCACCATCCAGTGCAGGAGCTCCGATGATAAGCTGTCAAACATGAGAATTCCGATCCCCAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTT‑CGCAACGTTCAAATCCGC‑TCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTGATGCCTGGGGAATTGGGAATTAATTCTTGAAGACGAAAGGGCCTCGTGATACGCCTA > CP009273/360033‑360329 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |