Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 434,663 A→C 87.8% I324S (ATC→AGC)  dxs ← 1‑deoxyxylulose‑5‑phosphate synthase, thiamine triphosphate‑binding, FAD‑requiring

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273434,6630AC87.8% 1316.5 / 113.3 380I324S (ATC→AGC) dxs1‑deoxyxylulose‑5‑phosphate synthase, thiamine triphosphate‑binding, FAD‑requiring
Reads supporting (aligned to +/- strand):  ref base A (26/21);  new base C (178/155);  total (204/176)
Fisher's exact test for biased strand distribution p-value = 8.76e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 380 total aligned reads displayed.

CGTGTTGCTCGGCAATTGCCACGTCGAAGTAGCGATCCGGGAATTTACGTGAAAACTCGACCATGCCGGAACCTTCACGCATCGCCGGAGTAATCGCCATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGATTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTGGAGGGATCAAATTTAGGCACGGCGTGGAAAGTGATCGGGTCTTTTTCTGCCGGTTCATAACCACGACCTTTTTTGGTCATGATATGCAGGAACTG  >  CP009273/434517‑434808
                                                                                                                                                  |                                                                                                                                                 
cgTGTTGCTCGGCAATTGCCACGTCGAAGTAGCGATCCGGGAATTTACGTGAAAACTCGACCATGCCGGAACCTTCACGCATCGCCGGAGTAATCGCCATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCtt                                                                                                                                                 <  2:4816170/149‑1 (MQ=255)
  tgtTGCTCGGCAATTGCCACGTCGAAGTAGCGATCCGGGAATTTACGTGAAAACTCGACCATGCCGGAACCTTCACGCATCGCCGGAGTAATCGCCATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCtttt                                                                                                                                               <  1:1002445/149‑1 (MQ=255)
  tgtTGCTCGGCAATTGCCACGTCGAAGTAGCGATCCGGGAATTTACGTGAAAACTCGACCATGCCGGAACCTTCACGCATCGCCGGAGTAATCGCCATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCtttt                                                                                                                                               >  1:2227227/1‑149 (MQ=255)
        tCGGCTATTGCCACGTCGAAGTAGCGATCCGGGAATTTACGTGAAAACTCGACCATGCCGGAACCTTCACGCATCGCCGGAGTAATCGCCATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATa                                                                                                                                         >  1:2584351/1‑149 (MQ=255)
        tCGGCAATTGCCACGTCGAAGTAGCGATCCGGGAATTTACGTGAAAACTCGACCATGCCGGAACCTTCACGCATCGCCGGAGTAATCGCCATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATa                                                                                                                                         <  1:992091/149‑1 (MQ=255)
         cGGCAATTGCCACGTCGAAGTAGCGATCCGGGAATTTACGTGAAAACTCGACCATGCCGGAACCTTCACGCATCGCCGGAGTAATCGCCATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAg                                                                                                                                        >  2:1259080/1‑149 (MQ=255)
           gCAATTGCCACGTCGAAGTAGCGATCCGGGAATTTACGTGAAAACTCGACCATGCCGGAACCTTCACGCATCGCCGGAGTAATCGCCATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCt                                                                                                                                      >  2:4940877/1‑149 (MQ=255)
           gCAATTGCCACGTCGAAGTAGCGATCCGGGAATTTACGTGAAAACTCGACCATGCCGGAACCTTCACGCATCGCCGGAGTAATCGCCATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCt                                                                                                                                      >  2:4784040/1‑149 (MQ=255)
           gCAATTGCCACGTCGAAGTAGCGATCCGGGAATTTACGTGAAAACTCGACCATGCCGGAACCTTCACGCATCGCCGGAGTAATCGCCATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGATTTTTGAATAGCt                                                                                                                                      <  1:3528792/149‑1 (MQ=255)
            cAAGTGCCACGTCGAAGTAGCGATCCGGGAATTTACGTGAAAACTCGACCATGCCGGAACCTTCACGCATCGCCGGAGTAATCGCCATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTc                                                                                                                                     <  2:3647985/149‑1 (MQ=255)
                    aCGTCGAAGTAGCGATCCGGGAATTTACGTGAAAACTCGACCATGCCGGAACCTTCACGCATCGCCGGAGTAATCGCCATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAAcc                                                                                                                             >  2:3687635/1‑149 (MQ=255)
                      gTCGAAGTAGCGATCCGGGAATTTACGTGAAAACTCGACCATGCCGGAACCTTCACGCATCGCCGGAGTAATCGCCATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAAccgc                                                                                                                           <  2:420023/149‑1 (MQ=255)
                      gTCGAAGTAGCGATCCGGGAATTTACGTGAAAACTCGACCATGCCGGAACCTTCACGCATCGCCGGAGTAATCGCCATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAAccgc                                                                                                                           >  2:2548773/1‑149 (MQ=255)
                      gTCGAAGTAGCGATCCGGGAATTTACGTGAAAACTCGACCATGCCGGAACCTTCACGCATCGCCGGAGTAATCGCCATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAAccgc                                                                                                                           >  2:3967984/1‑149 (MQ=255)
                         gAAGTAGCGATCCGGGAATTTACGTGAAAACTCGACCATGCCGGAACCTTCACGCATCGCCGGAGTAATCGCCATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACcgccgc                                                                                                                        <  2:3593755/149‑1 (MQ=255)
                         gAAGTAGCGATCCGGGAATTTACGTGAAAACTCGACCATGCCGGAACCTTCACGCATCGCCGGAGTAATCGCCATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACcgccgc                                                                                                                        <  2:1700478/149‑1 (MQ=255)
                         gAAGTAGCGATCCGGGAATTTACGTGAAAACTCGACCATGCCGGAACCTTCACGCATCGCCGGAGTAATCGCCATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACcgccgc                                                                                                                        >  1:5135001/1‑149 (MQ=255)
                          aaGTAGCGATCCGGGAATTTACGTGAAAACTCGACCATGCCGGAACCTTCACGCATCGCCGGAGTAATCGCCATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCt                                                                                                                       >  2:3443512/1‑149 (MQ=255)
                            gTAGCGATCCGGGAATTTACGTGAAAACTCGACCATGCCGGAACCTTCACGCATCGCCGGAGTAATCGCCATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTAc                                                                                                                     >  2:3328656/1‑149 (MQ=255)
                              aGCGATCCGGGAATTTACGTGAAAACTCGACCATGCCGGAACCTTCACGCATCGCCGGAGTAATCGCCATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACtt                                                                                                                   >  1:3705861/1‑149 (MQ=255)
                              aGCGATCCGGGAATTTACGTGAAAACTCGACCATGCCGGAACCTTCACGCATCGCCGGAGTAATCGCCATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACtt                                                                                                                   <  2:1788892/149‑1 (MQ=255)
                              aGCGATCCGGGAATTTACGTGAAAACTCGACCATGCCGGAACCTTCACGCATCGCCGGAGTAATCGCCATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACtt                                                                                                                   >  2:1509802/1‑149 (MQ=255)
                                  aTCCGGGAATTTACGTGAAAACTCGACCATGCCGGAACCTTCACGCATCGCCGGAGTAATCGCCATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCg                                                                                                               >  1:1656803/1‑149 (MQ=255)
                                      gggAATTTACGTGAAAACTCGACCATGCCGGAACCTTCACGCATCGCCGGAGTAATCGCCATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCaa                                                                                                           >  2:1128738/1‑149 (MQ=255)
                                        gAATTTACGTGAAAACTCGACCATGCCGGAACCTTCACGCATCGCCGGAGTAATCGCCATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAaac                                                                                                         >  2:3041062/1‑149 (MQ=255)
                                        gAATTTACGTGAAAACTCGACCATGCCGGAACCTTCACGCATCGCCGGAGTAATCGCCATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAaac                                                                                                         >  2:515304/1‑149 (MQ=255)
                                        gAATTTACGTGAAAACTCGACCATGCCGGAACCTTCACGCATCGCCGGAGTAATCGCCATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAaac                                                                                                         >  2:4032803/1‑149 (MQ=255)
                                          aTTTACGTGAAAACTCGACCATGCCGGAACCTTCACGCATCGCCGGAGTAATCGCCCTCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAaacaa                                                                                                       >  2:913859/1‑149 (MQ=255)
                                          aTTTACGTGAAAACTCGACCATGCCGGAACCTTCACGCATCGCCGGAGTAATCGCCATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGATTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAaacaa                                                                                                       >  1:1752405/1‑149 (MQ=255)
                                           tttACGTGAAAACTCGACCATGCCGGAACCTTCACGCATCGCCGGAGTAATCGCCATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAaacaac                                                                                                      <  2:2780944/149‑1 (MQ=255)
                                           tttACGTGAAAACTCGACCATGCCGGAACCTTCACGCATCGCCGGAGTAATCGCCATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAaacaac                                                                                                      <  1:937468/149‑1 (MQ=255)
                                           tttACGTGAAAACTCGACCATGCCGGAACCTTCACGCATCGCCGGAGTAATCGCCATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAaacaac                                                                                                      >  1:791517/1‑149 (MQ=255)
                                           tttACGTGAAAACTCGACCATGCCGGAACCTTCACGCATCGCCGGAGTAATCGCCATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAaacaac                                                                                                      >  1:654780/1‑149 (MQ=255)
                                           tttACGTGAAAACTCGACCATGCCGGAACCTTCACGCATCGCCGGAGTAATCGCCATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAaacaac                                                                                                      <  1:4940577/149‑1 (MQ=255)
                                               cGTGAAAACTCGACCATGCCGGAACCTTCACGCATCGCCGGAGTAATCGCCATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCt                                                                                                  >  1:1375150/1‑149 (MQ=255)
                                                gTGAAAACTCGACCATGCCGGAACCTTCACGCATCGCCGGAGTAATCGCCATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTg                                                                                                 >  2:735933/1‑149 (MQ=255)
                                                 tGAAAACTCGACCATGCCGGAACCTTCACGCATCGCCGGAGTAATCGCCATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTgg                                                                                                >  1:4486739/1‑149 (MQ=255)
                                                       cTCGACCATGCCGGAACCTTCACGCATCGCCGGAGTAATCGCCATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTGGAGGGAt                                                                                          >  1:2028020/1‑149 (MQ=255)
                                                          gACCATGCCGGAACCTTCACGCATCGCCGGAGTAATCGCCATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGATTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTGGAGGGATCaa                                                                                       >  2:1798895/1‑149 (MQ=255)
                                                           aCCATGCCGGAACCTTCACGCATCGCCGGAGTAATCGCCATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGGATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTGGAGGGATCaaa                                                                                      >  2:789534/1‑149 (MQ=255)
                                                           aCCATGCCGGAACCTTCACGCATCGCCGGAGTAATCGCCATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTGGAGGGATCaaa                                                                                      >  1:4770927/1‑149 (MQ=255)
                                                           aCCATGCCGGAACCTTCACGCATCGCCGGAGTAATCGCCATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGATTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTGGAGGGATCaaa                                                                                      >  2:195638/1‑149 (MQ=255)
                                                            ccATGCCGGAATCTTCACGCATCGCCGGAGTAATCGCCATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTGGAGGGATCAAAt                                                                                     <  2:3739472/149‑1 (MQ=255)
                                                            ccATGCCGGAACCTTCACGCATCGCCGGAGTAATCGCCATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTGGAGGGATCAAAt                                                                                     >  1:5016791/1‑149 (MQ=255)
                                                             cATGCCGGAACCTTCACGCATCGCCGGAGTAATCGCCATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTGGAGGGATCAAAtt                                                                                    <  2:2614068/149‑1 (MQ=255)
                                                               tGCCGGAACCTTCACGCATCGCCGGAGTAATCGCCATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTGGAGGGATCAAATTTa                                                                                  <  1:4562965/149‑1 (MQ=255)
                                                                gCCGGAACCTTCACGCATCGCCGGAGTAATCGCCATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTGGAGGGATCAAATTTAg                                                                                 <  1:1281197/149‑1 (MQ=255)
                                                                gCCGGAACCTTCACGCATCGCCGGAGTAATCGCCATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTGGAGGGATCAAATTTAg                                                                                 <  2:1391993/149‑1 (MQ=255)
                                                                 ccGGAACCTTCACGCATCGCCGGAGTAATCGCCATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTGGAGGGATCAAATTTAgg                                                                                <  1:2928009/149‑1 (MQ=255)
                                                                  cGGAACCTTCACGCATCGCCGGAGTAATCGCCATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTGGAGGGATCAAATTTAGGc                                                                               <  2:928416/149‑1 (MQ=255)
                                                                    gAACCTTCACGCATCGCCGGAGTAATCGCCATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTGGAGGGATCAAATTTAGGCAc                                                                             <  1:873347/149‑1 (MQ=255)
                                                                    gAACCTTCACGCATCGCCGGAGTAATCGCCATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTGGAGGGATCAAATTTAGGCAc                                                                             >  1:341798/1‑149 (MQ=255)
                                                                     aaCCTTCACGCATCGCCGGAGTAATCGCCATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTGGAGGGATCAAATTTAGGCACg                                                                            >  2:1164690/1‑149 (MQ=255)
                                                                     aaCCTTCACGCATCGCCGGAGTAATCGCCATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTGGAGGGATCAAATTTAGGCACg                                                                            >  1:2420422/1‑149 (MQ=255)
                                                                          tCACGCATCGCCGGAGTAATCGCCATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTGGAGGGATCAAATTTAGGCACGGCGTg                                                                       <  2:2919902/149‑1 (MQ=255)
                                                                             cGCATCGCCGGAGTAATCGCCATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTGGAGGGATCAAATTTAGGCACGGCGTGGaa                                                                    <  2:2232398/149‑1 (MQ=255)
                                                                               cATCGCCGGAGTAATCGCCATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTGGAGGGATCAAATTTAGGCACGGCGTGGAAAg                                                                  <  2:2731079/149‑1 (MQ=255)
                                                                                 tCGCCGGAGTAATCGCCATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGATTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAAAAAACGCTGGAGGGATCAAATTTAGGCACGGCGTGGAAAGTg                                                                >  1:4411469/1‑149 (MQ=255)
                                                                                   gCCGGAGTAATCGCCATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGATTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTGGAGGGATCAAATTTAGGCACGGCGTGGAAAGTGAt                                                              >  1:2727617/1‑149 (MQ=255)
                                                                                      ggAGTAATCGCCATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGATTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTGGAGGGATCAAATTTAGGCACGGCGTGGAAAGTGATCgg                                                           <  2:5077550/149‑1 (MQ=255)
                                                                                         gTAATCGCCATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTGGAGGGATCAAATTTAGGCACGGCGTGGAAAGTGATCGGGTc                                                        <  1:1513119/149‑1 (MQ=255)
                                                                                         gTAATCGCCATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTGGAGGGATCAAATTTAGGCACGGCGTGGAAAGTGATCGGGTc                                                        <  1:1512984/149‑1 (MQ=255)
                                                                                         gTAATCGCAATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCAAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTGGAGGGATCAAATTTAGGCACGGCGTGGAAAGTGATCGGGTc                                                        <  1:3871723/149‑1 (MQ=255)
                                                                                           aaTCGCCATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTGGAGGGATCAAATTTAGGCACGGCGTGGAAAGTGATCGGGTCtt                                                      >  1:445688/1‑149 (MQ=255)
                                                                                             tCGCCATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGATTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTGGAGGGATCAAATTTAGGCACGGCGTGGAAAGTGATCGGGTCtttt                                                    >  1:684216/1‑149 (MQ=255)
                                                                                              ctgcATCAGCTTGTTGTCTTTCTCTGCCGTTTCGCACAACCAGTCGACTTAGATTTTTGAATAGCTCGGAAAACCGCCTCTACTTTTCGGCAAACAACCGCTTGAGGGATAATATTTATGCACGGCTTGGAAATTGATGGGGTCttttt                                                   <  2:1932222/146‑1 (MQ=255)
                                                                                                  aTCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTGGAGGGATCAAATTTAGGCACGGCGTGGAAAGTGATCGGGTCTTTTTCTGc                                               >  1:311078/1‑149 (MQ=255)
                                                                                                   tCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTGGAGGGATCAAATTTAGGCACGGCGTGGAAAGTGATCGGGTCTTTTTCTGcc                                              <  1:1128738/149‑1 (MQ=255)
                                                                                                   tCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGATTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTGGAGGGATCAAATTTAGGCACGGCGTGGAAAGTGATCGGGTCTTTTTCTGcc                                              >  2:3859587/1‑149 (MQ=255)
                                                                                                      gCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTGGAGGGATCAAATTTAGGCACGGCGTGGAAAGTGATCGGGTCTTTTTCTGCCGGt                                           <  2:4342439/149‑1 (MQ=255)
                                                                                                      gCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGATTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTGGAGGGATCAAATTTAGGCACGGCGTGGAAAGTGATCGGGTCTTTTTCTGCCGGt                                           <  1:2315548/149‑1 (MQ=255)
                                                                                                        ttgttgTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTGGAGGGATCAAATTTAGGCACGGCGTGGAAAGTGATCGGGTCTTTTTCTGCCGGTTc                                         <  1:4076943/149‑1 (MQ=255)
                                                                                                        ttgttgTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTGGAGGGATCAAATTTAGGCACGGCGTGGAAAGTGATCGGGTCTTTTTCTGCCGGTTc                                         >  1:389766/1‑149 (MQ=255)
                                                                                                          gttgtCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTGGAGGGATCAAATTTAGGCACGGCGTGGAAAGTGATCGGGTCTTTTTCTGCCGGTTCAt                                       <  1:2833077/149‑1 (MQ=255)
                                                                                                            tgtCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTGGAGGGATCAAATTTAGGCACGGCGTGGAAAGTGATCGGGTCTTTTTCTGCCGGTTCATaa                                     <  2:4980842/149‑1 (MQ=255)
                                                                                                            tgtCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTGGAGGGATCAAATTTAGGCACGGCGTGGAAAGTGATCGGGTCTTTTTCTGCCGGTTCATaa                                     <  1:3324727/149‑1 (MQ=255)
                                                                                                             gtCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTGGAGGGATCAAATTTAGGCACGGCGTGGAAAGTGATCGGGTCTTTTTCTGCCGGTTCATAAc                                    <  2:630910/149‑1 (MQ=255)
                                                                                                              tCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTGGAGGGATCAAATTTAGGCACGGCGTGGAAAGTGATCGGGTCTTTTTCTGCCGGTTCATAAcc                                   >  1:3914307/1‑149 (MQ=255)
                                                                                                                tttCGCTGCCGTTTCGCACAACCAGTCGCCAAAGATTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTGGAGGGATCAAATTTAGGCACGGCGTGGAAAGTGATCGGGTCTTTTTCTGCCGGTTCATAACCAc                                 >  2:2837018/1‑149 (MQ=255)
                                                                                                                tttCGCTGCCGTTTCGCACAACCAGTCGCCAAAGATTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTGGAGGGATCAAATTTAGGCACGGCGTGGAAAGTGATCGGGTCTTTTTCTGCCGGTTCATAACCAc                                 >  2:2069368/1‑149 (MQ=255)
                                                                                                                   cGCTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTAGGCAAACAACCGCTGGAGGGATCAAATTTAGGCACGGCGTGGAAAGTGATCGGGTCTTTTTCTGCCGGTTCATAACCACGAc                              <  2:4308363/149‑1 (MQ=255)
                                                                                                                       gCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTGGAGGGATCAAATTTAGGCACGGCGTGGAAAGTGATCGGGTCTTTTTCTGCCGGTTCATAACCACGACCttt                          >  1:5171942/1‑149 (MQ=255)
                                                                                                                          gTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTGGAGGGATCAAATTTAGGCACGGCGTGGAAAGTGATCGGGTCTTTTTCTGCCGGTTCATAACCACGACCtttttt                       <  2:4869116/149‑1 (MQ=255)
                                                                                                                           tttCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTGGAGGGATCAAATTTAGGCACGGCGTGGAAAGTGATCGGGTCTTTTTCTGCCGGTTCATAACCACGACCTTTTTTg                      >  1:248652/1‑149 (MQ=255)
                                                                                                                           tttCGCACAACCAGTCGCCAAAGATTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTGGAGGGATCAAATTTAGGCACGGCGTGGAAAGTGATCGGGTCTTTTTCTGCCGGTTCATAACCACGACCTTTTTTg                      >  1:485917/1‑149 (MQ=255)
                                                                                                                            ttCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTGGAGGGATCAAATTTAGGCACGGCGTGGAAAGTGATCGGGTCTTTTTCTGCCGGTTCATAACCACGACCTTTTTTgg                     <  2:5009282/149‑1 (MQ=255)
                                                                                                                            ttCGCACAACCAGTCGCCAAAGATTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTGGAGGGATCAAATTTAGGCACGGCGTGGAAAGTGATCGGGTCTTTTTCTGCCGGTTCATAACCACGACCTTTTTTgg                     <  2:4535336/149‑1 (MQ=255)
                                                                                                                             tCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTGGAGGGATCAAATTTAGGCACGGCGTGGAAAGTGATCGGGTCTTTTTCTGCCGGTTCATAACCACGACCTTTTTTGGt                    >  1:1602374/1‑149 (MQ=255)
                                                                                                                             tCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTGGAGGGATCAAATTTAGGCACGGCGTGGAAAGTGATCGGGTCTTTTTCTGCCGGTTCATAACCACGACCTTTTTTGGt                    <  2:2165477/149‑1 (MQ=255)
                                                                                                                                cacaACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCCAACAACCGCTGGAGGGATCAAATTTAGGCACGGCGTGGAAAGTGATCGGGTCTTTTTCTGCCGGTTCATAACCACGACCTTTTTTGGTCAt                 <  2:4713869/149‑1 (MQ=255)
                                                                                                                                cacaACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTGGAGGGATCAAATTTAGGCACGGCGTGGAAAGTGATCGGGTCTTTTTCTGCCGGTTCATAACCACGACCTTTTTTGGTCAt                 <  2:14792/149‑1 (MQ=255)
                                                                                                                                cacaACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCCCTGGAGGGATCAAATTTAGGCACGGCGTGGAAAGTTATAGGGTCTTTTTATGACGGTTAATAACCACGACCTTTTTTGGTCAt                 >  1:1061470/1‑149 (MQ=255)
                                                                                                                                 acaACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTGGAGGGATCAAATTTAGGCACGGCGTGGAAAGTGATCGGGTCTTTTTCTGCCGGTTCATAACCACGACCTTTTTTGGTCATg                <  2:2599307/149‑1 (MQ=255)
                                                                                                                                    aCCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTGGAGGGATCAAATTTAGGCACGGCGTGGAAAGTGATCGGGTCTTTTTCTGCCGGTTCATAACCACGACCTTTTTTGGTCATGata             >  1:4622090/1‑149 (MQ=255)
                                                                                                                                     ccAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTGGAGGGATCAAATTTAGGCACGGCGTGGAAAGTGATCGGGTCTTTTTCTGCCGGTTCATAACCACGACCTTTTTTGGTCATGatat            >  1:1842438/1‑149 (MQ=255)
                                                                                                                                     ccAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTGGAGGGATCAAATTTAGGCACGGCGTGGAAAGTGATCGGGTCTTTTTCTGCCGGTTCATAACCACGACCTTTTTTGGTCATGatat            <  2:234194/149‑1 (MQ=255)
                                                                                                                                     ccAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTGGAGGGATCAAATTTAGGCACGGCGTGGAAAGTGATCGGGTCTTTTTCTGCCGGTTCATAACCACGACCTTTTTTGGTCATGatat            <  1:2961596/149‑1 (MQ=255)
                                                                                                                                       aGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTGGAGGGATCAAATTTAGGCACGGCGTGGAAAGTGATCGGGTCTTTTTCTGCCGGTTCATAACCACGACCTTTTTTGGTCATGATATGc          >  1:423297/1‑149 (MQ=255)
                                                                                                                                        gTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTGGAGGGATCAAATTTAGGCACGGCGTGGAAAGTGATCGGGTCTTTTTCTGCCGGTTCATAACCACGACCTTTTTTGGTCATGATATGCa         >  2:4564636/1‑149 (MQ=255)
                                                                                                                                        gTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTGGAGGGATCAAATTTAGGCACGGCGTGGAAAGTGATCGGGTCTTTTTCTGCCGGTTCATAACCACGACCTTTTTTGGTCATGATATGCa         >  2:2421700/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
CGTGTTGCTCGGCAATTGCCACGTCGAAGTAGCGATCCGGGAATTTACGTGAAAACTCGACCATGCCGGAACCTTCACGCATCGCCGGAGTAATCGCCATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGATTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTGGAGGGATCAAATTTAGGCACGGCGTGGAAAGTGATCGGGTCTTTTTCTGCCGGTTCATAACCACGACCTTTTTTGGTCATGATATGCAGGAACTG  >  CP009273/434517‑434808

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑