Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 535,589 G→T 5.5% M329I (ATG→ATT)  allB → allantoinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273535,5890GT 5.5% 1396.2 / 40.1 361M329I (ATG→ATT) allBallantoinase
Reads supporting (aligned to +/- strand):  ref base G (156/185);  new base T (8/12);  total (164/197)
Fisher's exact test for biased strand distribution p-value = 6.52e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

Only 100 of 367 total aligned reads displayed.

AAATCGGTACTCTGGCGAAGTGTTCACCGCCGATCCGCGATCTGGAAAACCAGAAAGGCATGTGGGAAAAACTGTTTAACGGTGAAATCGACTGCCTGGTTTCCGACCACTCTCCATGCCCGCCGGAAATGAAAGCCGGTAACATCATGAAAGCATGGGGCGGTATCGCCGGTCTGCAAAGCTGCATGGACGTGATGTTCGATGAAGCGGTACAGAAACGCGGTATGTCTCTGCCAATGTTCGGCAAATTAATGGCGACTAACGCAGCAGATATTTTCGGTCTGCAGCAAAAAGGCC  >  CP009273/535441‑535737
                                                                                                                                                    |                                                                                                                                                    
aaaTCGGTACTCTGGCGAAGTGTTCACCGCCGATCCGCGATCTGGAAAACCAGAAAGGCATGTGGGAAAAACTGTTTAACGGTGAAATCGACTGCCTGGTTTCCGACCACTCTCCATGCCCGCCGGAAATGAAAGCCGGTAACATCATg                                                                                                                                                      >  2:715892/1‑149 (MQ=255)
 aaTCGGTACTCTGGCGAAGTGTTCACCGCCGATCCGCGATCTGGAAAACCAGAAAGGCATGTGGGAAAAACTGTTTAACGGTGAAATCGACTGCCTGGTTTCCGACCACTCTCCATGCCCGCCGGAAATGAAAGCCGGTAACATCATGa                                                                                                                                                     <  2:1264118/149‑1 (MQ=255)
 aaTCGGTACTCTGGCGAAGTGTTCACCGCCGATCCGCGATCTGGAAAACCAGAAAGGCATGTGGGAAAAACTGTTTAACGGTGAAATCGACTGCCTGGTTTCCGACCACTCTCCATGCCCGCCGGAAATGAAAGCCGGTAACATCATGa                                                                                                                                                     >  1:4479039/1‑149 (MQ=255)
  aTCGGTACTCTGGCGAAGTGTTCACCGCCGATCCGCGATCTGGAAAACCAGAAAGGCATGTGGGAAAAACTGTTTAACGGTGAAATCGACTGCCTGGTTTCCGACCACTCTCCATGCCCGCCGGAAATGAAAGCCGGTAACATCATGaa                                                                                                                                                    <  1:2150448/149‑1 (MQ=255)
   tCGGTACTCTGGCGAAGTGTTCACCGCCGATCCGCGATCTGGAAAACCAGAAAGGCATGTGGGAAAAACTGTTTAACGGTGAAATCGACTGCCTGGTTTCCGACCACTCTCCATGCCCGCCGGAAATGAAAGCCGGTAACATCATGaaa                                                                                                                                                   <  1:4290529/149‑1 (MQ=255)
    cGGTACTCTGGCGAAGTGTTCACCGCCGATCCGCGATCTGGAAAACCAGAAAGGCATGTGGGAAAAACTGTTTAACGGTGAAATCGACTGCCTGGTTTCCGACCACTCTCCATGCCCGCCGGAAATGAAAGCCGGTAACATCATGAAAg                                                                                                                                                  >  2:2415799/1‑149 (MQ=255)
        aCTCTGGCGAAGTGTTCACCGCCGATCCGCGATCTGGAAAACCAGAAAGGCATGTGGGAAAAACTGTTTAACGGTGAAATCGACTGCCTGGTTTCCGACCACTCTCCATGCCCGCCGGAAATGAAAGCCGGTAACATCATGAAAGCATg                                                                                                                                              >  2:2007578/1‑149 (MQ=255)
            tGGCGAAGTGTTCACCGCCGATCCGCGATCTGGAAAACCAGAAAGGCATGTGGGAAAAACTGTTTAACGGTGAAATCGACTGCCTGGTTTCCGACCACTCTCCATGCCCGCCGGAACTGAAAGCCGGTAACATCATGAAAGCATGGGGc                                                                                                                                          >  2:1914549/1‑149 (MQ=255)
            tGGCGAAGTGTTCACCGCCGATCCGCGATCTGGAAAACCAGAAAGGCATGTGGGAAAAACTGTTTAACGGTGAAATCGACTGCCTGGTTTCCGACCACTCTCCATGCCCGCCGGAAATGAAAGCCGGTAACATCATGAAAGCATGGGGc                                                                                                                                          >  2:2976946/1‑149 (MQ=255)
            tGGCGAAGTGTTCACCGCCGATCCGCGATCTGGAAAACCAGAAAGGCATGTGGGAAAAACTGTTTAACGGTGAAATCGACTGCCTGGTTTCCGACCACTCTCCATGCCCGCCGGAAATGAAAGCCGGTAACATCATGAAAGCATGGGGc                                                                                                                                          >  2:2180464/1‑149 (MQ=255)
               cGAAGTGTTCACCGCCGATCCGCGATCTGGAAAACCAGAAAGGCATGTGGGAAAAACTGTTTAACGGTGAAATCGACTGCCTGGTTTCCGACCACTCTCCATGCCCGCCGGAAATGAAAGCCGGTAACATCATGAAAGCATGGGGCGGt                                                                                                                                       <  1:1656903/149‑1 (MQ=255)
                gAAGTGTTCACCGCCGATCCGCGATCTGGAAAACCAGAAAGGCATGTGGGAAAAACTGTTTAACGGTGAAATCGACTGCCTGGTTTCCGACCACTCTCCATGCCCGCCGGAAATGAAAGCCGGTAACATCATGAAAGCATGGGGCGGTa                                                                                                                                      <  1:1998301/149‑1 (MQ=255)
                 aaGTGTTCACCGCCGATCCGCGATCTGGAAAACCAGAAAGGCATGTGGGAAAAACTGTTTAACGGTGAAATCGACTGCCTGGTTTCCGACCACTCTCCATGCCCGCCGGAAATGAAAGCCGGTAACATCATGAAAGCATGGGGCGGTAt                                                                                                                                     >  2:4166582/1‑149 (MQ=255)
                 aaGTGTTCACCGCCGATCCGCGATCTGGAAAACCAGAAAGGCATGTGGGAAAAACTGTTTAACGGTGAAATCGACTGCCTGGTTTCCGACCACTCTCCATGCCCGCCGGAAATGAAAGCCGGTAACATCATGAAAGCATGGGGCGGTAt                                                                                                                                     >  1:2471524/1‑149 (MQ=255)
                    tgtTCACCGCCGATCCGCGATCTGGAAAACCAGAAAGGCATGTGGGAAAAACTGTTTAACGGTGAAATCGACTGCCTGGTTTCCGACCACTCTCCATGCCCGCCGGAAATGAAAGCCGGTAACATCATTAAAGCATGGGGCGGTATCGc                                                                                                                                  <  2:275227/149‑1 (MQ=255)
                    tgtTCACCGCCGATCCGCGATCTGGAAAACCAGAAAGGCATGTGGGAAAAACTGTTTAACGGTGAAATCGACTGCCTGGTTTCCGACCACTCTCCATGCCCGCCGGAAATGAAAGCCGGTAACATCATGAAAGCATGGGGCGGTATCGc                                                                                                                                  <  1:1210168/149‑1 (MQ=255)
                          ccgccgATCCGCGATCTGGAAAACCAGAAAGGCATGTGGGAAAAACTGTTTAACGGTGAAATCGACTGCCTGGTTTCCGACCACTCTCCATGCCCGCCGGAAATGAAAGCCGGTAACATCATGAAAGCATGGGGCGGTATCGCCGGTCt                                                                                                                            <  1:1017487/149‑1 (MQ=255)
                            gccgATCCGCGATCTGGAAAACCAGAAAGGCATGTGGGAAAAACTGTTTAACGGTGAAATCGACTGCCTGGTTTCCGACCACTCTCCATGCCCGCCGGAAATGAAAGCCGGTAACATCATGAAAGCATGGGGCGGTATCGCCGGTCTGc                                                                                                                          <  1:3456441/149‑1 (MQ=255)
                            gccgATCCGCGATCTGGAAAACCAGAAAGGCATGTGGGAAAAACTGTTTAACGGTGAAATCGACTGCCTGGTTTCCGACCACTCTCCATGCCCGCCGGAAATGAAAGCCGGTAACATCATGAAAGCATGGGGCGGTATCGCCGGTCTGc                                                                                                                          >  1:3926988/1‑149 (MQ=255)
                            gccgATCCGCGATCTGGAAAACCAGAAAGGCATGTGGGAAAAACTGTTTAACGGTGAAATCGACTGCCTGGTTTCCGACCACTCTCCATGCCCGCCGGAAATGAAAGCCGGTAACATCATGAAAGCATGGGGCGGTATCGCCGGTCTGc                                                                                                                          <  1:834789/149‑1 (MQ=255)
                               gATCCGCGATCTGGAAAACCAGAAAGGCATGTGGGAAATACTGTTTAACGGTGAAATCGACTGCCTGGTTTCCGACCACTCTCCATGCCCGCCGGAAATGAAAGCCGGTAACATCATGAAAGCATGGGGCGGTATCGCCGGTCTGCaaa                                                                                                                       >  1:4286206/1‑149 (MQ=255)
                                aTCCGCGATCTGGAAAACCAGAAAGGCATGTGGGAAAAACTGTTTAACGGTGAAATCGACTGCCTGGTTTCCGACCACTCTCCATGCCCGCCGGAAATGAAAGCCGGTAACATCATGAAAGCATGGGGCGGTATCGCCGGTCTGCAAAg                                                                                                                      >  1:2876879/1‑149 (MQ=255)
                                 tCCGCGATCTGGAAAACCAGAAAGGCATGTGGGAAAAACTGTTTAACGGTGAAATCGACTGCCTGGTTTCCGACCACTCTCCATGCCCGCCGGAAATGAAAGCCGGTAACATCATGAAAGCATGGGGCGGTATCGCCGGTCTGCAAAGc                                                                                                                     >  2:4850324/1‑149 (MQ=255)
                                 tCCGCGATCTGGAAAACCAGAAAGGCATGTGGGAAAAACTGTTTAACGGTGAAATCGACTGCCTGGTTTCCGACCACTCTCCATGCCCGCCGGAAATGAAAGCCGGTAACATCATGAAAGCATGGGGCGGTATCGCCGGTCTGCAAAGc                                                                                                                     <  1:5062068/149‑1 (MQ=255)
                                 tCCGCGATCTGGAAAACCAGAAAGGCATGTGGGAAAAACTGTTTAACGGTGAAATCGACTGCCTGGTTTCCGACCACTCTCCATGCCCGCCGGAAATGAAAGCCGGTAACATCATGAAAGCATGGGGCGGTATCGCCGGTCTGCAAAGc                                                                                                                     >  2:503014/1‑149 (MQ=255)
                                    gcgATCTGGAAAACCAGAAAGGCATGTGGGAAAAACTGTTTAACGGTGAAATCGACTGCCTGGTTTCCGACCACTCTCCATGCCCGCCGGAAATGAAAGCCGGTAACATCATGAAAGCATGGGGCGGTATCGCCGGTCTGCAAAGCTGc                                                                                                                  >  2:1609362/1‑149 (MQ=255)
                                    gcgATCTGGAAAACCAGAAAGGCATGTGGGAAAAACTGTTTAACGGTGAAATCGACTGCCTGGTTTCCGACCACTCTCCATGCCCGCCGGAAATGAAAGCCGGTAACATCATGAAAGCATGGGGCGGTATCGCCGGTCTGCAAAGCTGc                                                                                                                  <  2:943994/149‑1 (MQ=255)
                                    gcgATCTGGAAAACCAGAAAGGCATGTGGGAAAAACTGTTTAACGGTGAAATCGACTGCCTGGTTTCCGACCACTCTCCATGCCCGCCGGAAATGAAAGCCGGTAACATCATGAAAGCATGGGGCGGTATCGCCGGTCTGCAAAGCTGc                                                                                                                  >  2:3864056/1‑149 (MQ=255)
                                        tCTGGAAAACCAGAAAGGCATGTGGGAAAAACTGTTTAACGGTGAAATCGACTGCCTGGTTTCCGACCACTCTCCATGCCCGCCGGAAATGAAAGCCGGTAACATCATGAAAGCATGGGGCGGTATCGCCGGTCTGCAAAGCTGCATgg                                                                                                              <  1:1636553/149‑1 (MQ=255)
                                        tCTGGAAAACCAGAAAGGCATGTGGGAAAAACTGTTTAACGGTGAAATCGACTGCCTGGTTTCCGACCACTCTCCATGCCCGCCGGAAATGAAAGCCGGTAACATCATGAAAGCATGGGGCGGTATCGCCGGTCTGCAAAGCTGCATgg                                                                                                              <  1:4254062/149‑1 (MQ=255)
                                         cTGGAAAACCAGAAAGGCATGTGGGAAAAACTGTTTAACGGTGAAATCGACTGCCTGGTTTCCGACCACTCTCCATGCCCGCCGGAAATGAAAGCCGGTAACATCATGAAAGCATGGGGCGGTATCGCCGGTCTGCAAAGCTGCATGGa                                                                                                             <  2:419010/149‑1 (MQ=255)
                                          tGGAAAACCAGAAAGGCATGTGGGAAAAACTGTTTAACGGTGAAATCGACTGCCTGGTTTCCGACCACTCTCCATGCCCGCCGGAGATGAAAGCCGGTAACATCATGAAAGCATGGGGCGGTATCGCCGGTCTGCAAAGCTGCATGGAc                                                                                                            <  2:1511502/149‑1 (MQ=255)
                                            gAAAACCAGAAAGGCATGTGGGAAAAACTGTTTAACGGTGAAATCGACTGCCTGGTTTCCGACCACTCTCCATGCCCGCCGGAAATGAAAGCCGGTAACATCATGAAAGCATGGGGCGGTATCGCCGGTCTGCAAAGCTGCATGGACGt                                                                                                          <  1:4961247/149‑1 (MQ=255)
                                             aaaaCCAGAAAGGCATGTGGGAAAAACTGTTTAACGGTGAAATCGACTGCCTGGTTTCCGACCACTCTCCATGCCCGCCGGAAATGAAAGCCGGTAACATCATGAAAGCATGGGGCGGTATCGCCGGTCTGCAAAGCTGCATGGACGTg                                                                                                         >  1:2634846/1‑149 (MQ=255)
                                             aaaaCCAGAAAGGCATGTGGGAAAAACTGTTTAACGGTGAAATCGACTGCCTGGTTTCCGACCACTCTCCATGCCCGCCGGAAATGAAAGCCGGTAACATCATGAAAGCATGGGGCGGTATCGCCGGTCTGCAAAGCTGCATGGACGTg                                                                                                         >  1:4603548/1‑149 (MQ=255)
                                               aaCCAGAAAGGCATGTGGGAAAAACTGTTTAACGGTGAAATCGACTGCCTGGTTTCCGACCACTCTCCATGCCCGCCGGAAATGAAAGCCGGTAACATCATGAAAGCATGGGGCGGTATCGCCGGTCTGCAAAGCTGCATGGACGTGAt                                                                                                       <  2:2273021/149‑1 (MQ=255)
                                                aCCAGAAAGGCATGTGGGAAAAACTGTTTAACGGTGAAATCGACTGCCTGGTTTCCGACCACTCTCCATGCCCGCCGGAAATGAAAGCCGGTAACATCATGAAAGCATGGGGCGGTATCGCCGGTCTGCAAAGCTGCATGGACGTGATg                                                                                                      <  2:3803547/149‑1 (MQ=255)
                                                 ccAGAAAGGCATGTGGGAAAAACTGTTTAACGGTGAAATCGACTGCCTGGTTTCCGACCACTCTCCATGCCCGCCGGAAATGAAAGCCGGTAACATCATGAAAGCATGGGGCGGTATCGCCGGTCTGCAAAGCTGCATGGACGTGATGt                                                                                                     <  1:4667942/149‑1 (MQ=255)
                                                 ccAGAAAGGCATGTGGGAAAAACTGTTTAACGGTGAAATCGACTGCCTGGTTTCCGACCACTCTCCATGCCCGCCGGAAATGAAAGCCGGTAACATCATGAAAGCATGGGGCGGTATCGCCGGTCTGCAAAGCTGCATGGACGTGATGt                                                                                                     <  1:4674834/149‑1 (MQ=255)
                                                     acaGGCATGTGGGAAAAACTGTTTAACGGTGAAATCGACTGCCTGGTTTCCGACCACTCTCCATGCCCGCCGGAAATGAAAGCCGGTAACATCATGAAAGCATGGGGCGGTATCGCCGGTCTGCAAAGCTGCATGGACGTGATGTTCGa                                                                                                 <  1:4485901/147‑1 (MQ=255)
                                                      aaGGCATGTGGGAAAAACTGTTTAACGGTGAAATCGACTGCCTGGTTTCCGACCACTCTCCATGCCCGCCGGAAATGAAAGCCGGTAACATCATGAAAGCATGGGGCGGTATCGCCGGTCTGCAAAGCTGCATGGACGTGATGTTCGAt                                                                                                >  2:1767044/1‑149 (MQ=255)
                                                      aaGGCATGTGGGAAAAACTGTTTAACGGTGAAATCGACTGCCTGGTTTCCGACCACTCTCCATGCCCGCCGGAAATGAAAGCCGGTAACATCATGAAAGCATGGGGCGGTATCGCCGGTCTGCAAAGCTGCATGGACGTGATGTTCGAt                                                                                                <  1:2370019/149‑1 (MQ=255)
                                                      aaGGCATGTGGGAAAAACTGTTTAACGGTGAAATCGACTGCCTGGTTTCCGACCACTCTCCATGCCCGCCGGAAATGAAAGCCGGTAACATCATGAAAGCATGGGGCGGTATCGCCGGTCTGCAAAGCTGCATGGACGTGATGTTCGAt                                                                                                >  1:3378765/1‑149 (MQ=255)
                                                       aGGCATGTGGGAAAAACTGTTTAACGGTGAAATCGACTGCCTGGTTTCCGACCACTCTCCATGCCCGCCGGAAATGAAAGCCGGTAACATCATGAAAGCATGGGGCGGTATCGCCGGTCTGCAAAGCTGCATGGACGTGATGTTCGATg                                                                                               >  2:640723/1‑149 (MQ=255)
                                                          cATGTGGGAAAAACTGTTTAACGGTGAAATCGACTGCCTGGTTTCCGACCACTCTCCATGCCCGCCGGAAATGAAAGCCGGTAACATCATGAAAGCATGGGGCGGTATCGCCGGTCTGCAAAGCTGCATGGACGTGATGTTCGATGAAg                                                                                            <  2:109588/149‑1 (MQ=255)
                                                           aTGTGGGAAAAACTGTTTAACGGTGAAATCGACTGCCTGGTTTCCGACCACTCTCCATGCCCGCCGGAAATGAAAGCCGGTAACATCATGAAAGCATGGGGCGGTATCGCCGGTCTGCAAAGCTGCATGGACGTGATGTTCGATGAAGc                                                                                           >  1:3882815/1‑149 (MQ=255)
                                                             gtgGGAAAAACTGTTTAACGGTGAAATCGACTGCCTGGTTTCCGACCACTCTCCATGCCCGCCGGAAATGAAAGCCGGTAACATCATGAAAGCATGGGGCGGTATCGCCGGTCTGCAAAGCTGCATGGACGTGATGTTCGATGAAGCgg                                                                                         <  1:3155580/149‑1 (MQ=255)
                                                               gggAAAAACTGTTTAACGGTGAAATCGACTGCCTGGTTTCCGACCACTCTCCATGCCCGCCGGAAATGAAAGCCGGTAACATCATGAAAGCATGGGGCGGTATCGCCGGTCTGCAAAGCTGCATGGACGTGATGTTCGATGAAGCGGTa                                                                                       >  1:3580996/1‑149 (MQ=255)
                                                               gggAAAAACTGTTTAACGGTGAAATCGACTGCCTGGTTTCCGACCACTCTCCATGCCCGCCGGAAATGAAAGCCGGTAACATCATGAAAGCATGGGGCGGTATCGCCGGTCTGCAAAGCTGCATGGACGTGATGTTCGATGAAGCGGTa                                                                                       >  2:3064159/1‑149 (MQ=255)
                                                                   aaaaCTGTTTAACGGTGAAATCGACTGCCTGGTTTCCGACCACTCTCCATGCCCGCCGGAAATGAAAGCCGGTAACATCATGAAAGCATGGGGCGGTATCGCCGGTCTGCAAAGCTGCATGGACGTGATGTTCGATGAAGCGGTACAGa                                                                                   >  1:980040/1‑149 (MQ=255)
                                                                   aaaaCTGTTTAACGGTGAAATCGACTGCCTGGTTTCCGACCACTCTCCATGCCCGCCGGAAATGAAAGCCGGTAACATCATGAAAGCATGGGGCGGTATCGCCGGTCTGCAAAGCTGCATGGACGTGATGTTCGATGAAGCGGTACAGa                                                                                   >  1:1376300/1‑149 (MQ=255)
                                                                    aaaCTGTTTAACGGTGAAATCGACTGCCTGGTTTCCGACCACTCTCCATGCCCGCCGGAAATGAAAGCCGGTAACATCATGAAAGCATGGGGCGGTATCGCCGGTCTGCAAAGCTGCATGGACGTGATGTTCGATGAAGCGGTACAGaa                                                                                  >  1:3017166/1‑149 (MQ=255)
                                                                      aCTGTTTAACGGTGAAATCGACTGCCTGGTTTCCGACCACTCTCCATGCCCGCCGGAAATGAAAGCCGGTAACATCATGAAAGCATGGGGCGGTATCGCCGGTCTGCAAAGCTGCATGGACGTGATGTTCGATGAAGCGGTACAGAAAc                                                                                >  2:760049/1‑149 (MQ=255)
                                                                          tttATCGGTGAAATCGACTGCATGGTTTCCGACCTCTCTCCATGCCCGCCGGAAATGAAAGCCGGTAACATCATGAAAGCATGGGGCGGTATCGCCTGTCTGCAAAGCTGCATGGACTTGATGTTCGATGAAGCGGTACAGAAACGCgg                                                                            <  2:1408009/149‑1 (MQ=255)
                                                                          tttAACGGTGAAATCGACTGCCTGGTTTCCGACCACTCTCCATGCCCGCCGGAAATGAAAGCCGGTAACATCATGAAAGCATGGGGCGGTATCGCCGGTCTGCAAAGCTGCATGGACGTGATGTTCGATGAAGCGGTACAGAAACGCgg                                                                            <  2:2839763/149‑1 (MQ=255)
                                                                          tttAACGGTGAAATCGACTGCCTGGTTTCCGACCACTCTCCATGCCCGCCGGAAATGAAAGCCGGTAACATCATGAAAGCATGGGGCGGTATCGCCGGTCTGCAAAGCTGCATGGACGTGATGTTCGATGAAGCGGTACAGAAACGCgg                                                                            <  1:4339440/149‑1 (MQ=255)
                                                                           ttAACGGTGAAATCGACTGCCTGGTTTCCGACCACTCTCCATGCCCGCCGGAAATGAAAGCCGGTAACATCATGAAAGCATGGGGCGGTATCGCCGGTCTGCAAAGCTGCATGGACGTGATGTTCGATGAAGCGGTACAGAAACGCGGt                                                                           <  1:4040561/149‑1 (MQ=255)
                                                                              aCGGTGAAATCGACTGCCTGGTTTCCGACCACTCTCCATGCCCGCCGGAAATGAAAGCCGGTAACATCATGAAAGCATGGGGCGGTATCGCCGGTCTGCAAAGCTGCATGGACGTGATGTTCGATGAAGCGGTACAGAAACGCGGTATg                                                                        <  2:3060869/149‑1 (MQ=255)
                                                                                ggTGAAATCGACTGCCTGGTTTCCGACCACTCTCCATGCCCGCCGGAAATGAAAGCCGGTAACATCATGAAAGCATGGGGCGGTATCGCCGGTCTGCAAAGCTGCATGGACGTGATGTTCGATGAAGCGGTACAGAAACGCGGTATGtc                                                                      <  2:2264034/149‑1 (MQ=255)
                                                                                 gTGAAATCGACTGCCTGGTTTCCGACCACTCTCCATGCCCGCCGGAAATGAAAGCCGGTAACATCATGAAAGCATGGGGCGGTATCGCCGGTCTGCAAAGCTGCATGGACGTGATGTTCGATGAAGCGGTACAGAAACGCGGTATGtct                                                                     >  1:3547761/1‑149 (MQ=255)
                                                                                 gTGAAATCGACTGCCTGGTTTCCGACCACTCTCCATGCCCGCCGGAAATGAAAGCCGGTAACATCATGAAAGCATGGGGCGGTATCGCCGGTCTGCAAAGCTGCATGGACGTGATGTTCGATGAAGCGGTACAGAAACGCGGTATGtct                                                                     >  2:903171/1‑149 (MQ=255)
                                                                                  tGAAATCGACTGCCTGGTTTCCGACCACTCTCCATGCCCGCCGGAAATGAAAGCCGGTAACATCATGAAAGCATGGGGCGGTATCGCCGGTCTGCAAAGCTGCATGGACGTGATGTTCGATGAAGCGGTACAGAAACGCGGTATGtctc                                                                    >  1:4971550/1‑149 (MQ=255)
                                                                                    aaaTCGACTGCCTGGTTTCCGACCACTCTCCATGCCCGCCGGAAATGAAAGCCGGTAACATCATGAAAGCATGGGGCGGTATCGCCGGTCTGCAAAGCTGCATGGACGTGATGTTCGATGAAGCGGTACAGAAACGCGGTATGTCTCTg                                                                  >  2:409306/1‑149 (MQ=255)
                                                                                     aaTCGACTGCCTGGTTTCCGACCACTCTCCATGCCCGCCGGAAATGAAAGCCGGTAACATCATGAAAGCATGGGGCGGTATCGCCGGTCTGCAAAGCTGCATGGACGTGATGTTCGATGAAGCGGTACAGAAACGCGGTATGTCTCTGc                                                                 >  1:5238707/1‑149 (MQ=255)
                                                                                       tCGACTGCCTGGTTTCCGACCACTCTCCATGCCCGCCGGAAATGAAAGCCGGTAACATCATGAAAGCATGGGGCGGTATCGCCGGTCTGCAAAGCTGCATGGACGTGATGTTCGATGAAGCGGTACAGAAACGCGGTATGTCTCTGCCa                                                               >  1:1562140/1‑149 (MQ=255)
                                                                                        cGACTGCCTGGGTTCCGACCACTCTCCATGCCCGCCGGAAATGAAAGCCGGTAACATCATGAAAGCATGGGGCGGTATCGCCGGTCTGCAAAGCTGCATGGACGTGATGTTCGATGAAGCGGTACAGAAACGCGGTATGTCTCTGCCaa                                                              <  2:3746159/149‑1 (MQ=255)
                                                                                           cTGCCTGGTTTCCGACCACTCTCCATGCCCGCCGGAAATGAAAGCCGGTAACATCATGAAAGCATGGGGCGGTATCGCCGGTCTGCAAAGCTGCATGGACGTGATGTTCGATGAAGCGGTACAGAAACGCGGTATGTCTCTGCCAATGt                                                           <  1:2094623/149‑1 (MQ=255)
                                                                                            tGCCTGGTTTCCGACCACTCTCCATGCCCGCCGGAAATGAAAGCCGGTAACATCATGAAAGCATGGGGCGGTATCGCCGGTCTGCAAAGCTGCATGGACGTGATGTTCGATGAAGCGGTACAGAAACGCGGTATGTCTCTGCCAATGtt                                                          >  1:330099/1‑149 (MQ=255)
                                                                                             gCCTGGTTTCCGACCACTCTCCATGCCCGCCGGAAATGAAAGCCGGTAACATCATGAAAGCATGGGGCGGTATCGCCGGTCTGCAAAGCTGCATGGACGTGATGTTCGATGAAGCGGTACAGAAACGCGGTATGTCTCTGCCAATGTTc                                                         <  1:220830/149‑1 (MQ=255)
                                                                                              ccTGGTTTCCGACCACTCTCCATGCCCGCCGGAAATGAAAGCCGGTAACATCATGAAAGCATGGGGCGGTATCGCCGGTCTGCAAAGCTGCATGGACGTGATGTTCGATGAAGCGGTACAGAAACGCGGTATGTCTCTGCCAATGTTCg                                                        >  2:2095442/1‑149 (MQ=255)
                                                                                              ccTGGTTTCCGACCACTCTCCATGCCCGCCGGAAATGAAAGCCGGTAACATCATGAAAGCATGGGGCGGTATCGCCGGTCTGCAAAGCTGCATGGACGTGAGGTTCGATGAAGCGGTACAGAAACGCGGTATGTCTCTGCCAATGTTCg                                                        <  2:268751/149‑1 (MQ=255)
                                                                                                tGGTTTCCGACCACTCTCCATGCCCGCCGGAAATGAAAGCCGGTAACATCATGAAAGCATGGGGCGGTATCGCCGGTCTGCAAAGCTGCATGGACGTGATGTTCGATGAAGCGGTACAGAAACGCGGTATGTCTCTGCCAATGTTCGGc                                                      <  2:4286575/149‑1 (MQ=255)
                                                                                                 ggTTTCCGACCACTCTCCATGCCCGCCGGAAATGAAAGCCGGTAACATCATGAAAGCATGGGGCGGTATCGCCGGTCTGCAAAGCTGCATGGACGTGATGTTCGATGAAGCGGTACAGAAACGCGGTATGTCTCTGCCAATGTTCGGCa                                                     >  2:1742087/1‑149 (MQ=255)
                                                                                                  gTTTCCGACCACTCTCCATGCCCGCCGGAAATGAAAGCCGGTAACATCATGAAAGCATGGGGCGGTATCGCCGGTCTGCAAAGCTGCATGGACGTGATGTTCGATGAAGCGGTACAGAAACGCGGTATGTCTCTGCCAATGTTCGGCaa                                                    >  2:4530247/1‑149 (MQ=255)
                                                                                                  gTTTCCGACCACTCTCCATGCCCGCCGGAAATGAAAGCCGGTAACATCATGAAAGCATGGGGCGGTATCGCCGGTCTGCAAAGCTGCATGGACGTGATGTTCGATGAAGCGGTACAGAAACGCGGTATGTCTCTGCCAATGTTCGGCaa                                                    <  1:3778637/149‑1 (MQ=255)
                                                                                                       cGACCACTCTCCATGCCCGCCGGAAATGAAAGCCGGTAACATCATGAAAGCATGGGGCGGTATCGCCGGTCTGCAAAGCTGCATGGACGTGATGTTCGATGAAGCGGTACAGAAACGCGGTATGTCTCTGCCAATGTTCGGCAAATTaa                                               >  1:1153389/1‑149 (MQ=255)
                                                                                                          ccACTCTCCATGCCCGCCGGAAATGAAAGCCGGTAACATCATGAAAGCATGGGGCGGTATCGCCGGTCTGCAAAGCTGCATGGACGTGATGTTCGATGAAGCGGTACAGAAACGCGGTATGTCTCTGCCAATGTTCGGCAAATTAATgg                                            >  1:2262860/1‑149 (MQ=255)
                                                                                                           cACTCTCCATGCCCGCCGGAAATGAAAGCCGGTAACATCATGAAAGCATGGGGCGGTATCGCCGGTCTGCAAAGCTGCATGGACGTGATGTTCGATGAAGCGGTACAGAAACGCGGTATGTCTCTGCCAATGTTCGGCAAATTAATGGc                                           >  2:3717725/1‑149 (MQ=255)
                                                                                                             ctctCCATGCCCGCCGGAAATGAAAGCCGGTAACATCATGAAAGCATGGGGCGGTATCGCCGGTCTGCAAAGCTGCATGGACGTGATGTTCGATGAAGCGGTACAGAAACGCGGTATGTCTCTGCCAATGTTCGGCAAATTAATGGCGa                                         >  2:3301031/1‑149 (MQ=255)
                                                                                                              tctcCATGCCCGCCGGAAATGAAAGCCGGTAACATCATGAAAGCATGGGGCGGTATCGCCGGTCTGCAAAGCTGCATGGACGTGATGTTCGATGAAGCGGTACAGAAACGCGGTATGTCTCTGCCAATGTTCGGCAAATTAATGGCGAc                                        <  1:1040827/149‑1 (MQ=255)
                                                                                                                 ccATGCCCGCCGGAAATGAAAGCCGGTAACATCATTAAAGCATGGGGCGGTATCGCCGGTCTGCAAAGCTGCATGGACGTGATGTTCGATGAAGCGGTACAGAAACGCGGTATGTCTCTGCCAATGTTCGGCAAATTAATGGCGACTaa                                     >  2:1155129/1‑149 (MQ=255)
                                                                                                                 ccAGGCCCGCCGGAAATGAAAGCCGGTAACATCATGAAAGCATGGGGCGGTATCGCCGGTCTGCAAAGCTGCATGGACGTGATGTTCGATGAAGCGGTACAGAAACGCGGTATGTCTCTGCCAATGTTCGGCAAATTAATGGCGACTaa                                     <  2:4631498/149‑1 (MQ=255)
                                                                                                                   aTGCCCGCCGGAAATGAAAGCCGGTAACATCATGAAAGCATGGGGCGGTATCGCCGGTCTGCAAAGCTGCATGGACGTGATGTTCGATGAAGCGGTACAGAAACGCGGTATGTCTCTGCCAATGTTCGGCAAATTAATGGCGACTAACg                                   >  2:2621092/1‑149 (MQ=255)
                                                                                                                       ccgccgGAAATGAAAGCCGGTAACATCATGAAAGCATGGGGCGGTATCGCCGGTCTGCAAAGCTGCATGGACGTGATGTTCGATGAAGCGGTACAGAAACGCGGTATGTCTCTGCCAATGTTCGGCAAATTAATGGCGACTAACgcagc                               <  2:4479237/149‑1 (MQ=255)
                                                                                                                       ccgccgGAAATGAAAGCCGGTAACATCATGAAAGCATGGGGCGGTATCGCCGGTCTGCAAAGCTGCATGGACGTGATGTTCGATGAAGCGGTACAGAAACGCGGTATGTCTCTGCCAATGTTCGGCAAATTAATGGCGACTAACgcagc                               <  2:1388293/149‑1 (MQ=255)
                                                                                                                          gcgGAAATGAAAGCCGGTAACATCATGAAAGCATGGGGCGGTATCGCCGGTCTGCAAAGCTGCATGGACGTGATGTTCGATGAAGCGGTACAGAAACGCGGTATGTCTCTGCCAATGTTCGGCAAATTAATGGCGACTAACGCAGCAGa                            <  2:3547208/148‑1 (MQ=255)
                                                                                                                            ggAAATGAAAGCCGGTAACATCATGAAAGCATGGGGCGGTATCGCCGGTCTGCAAAGCTGCATGGACGTGATGTTCGATGAAGCGGTACAGAAACGCGGTATGTCTCTGCCAATGTTCGGCAAATTAATGGCGACTAACGCAGCAGata                          <  1:2717024/149‑1 (MQ=255)
                                                                                                                              aaaTGAAAGCCGGTAACATCATGAAAGCATGGGGCGGTATCGCCGGTCTGCAAAGCTGCATGGACGTGATGTTCGATGAAGCGGTACAGAAACGCGGTATGTCTCTGCCAATGTTCGGCAAATTAATGGCGACTAACGCAGCAGATAtt                        <  1:2601387/149‑1 (MQ=255)
                                                                                                                                  gAAAGCCGGTAACATCATGAAAGCATGGGGCGGTATCGCCGGTCTGCAAAGCTGCATGGACGTGATGTTCGATGAAGCGGTACAGAAACGCGGTATGTCTCTGCCAATGTTCGGCAAATTAATGGCGACTAACGCAGCAGATATTTTCg                    >  2:1054949/1‑149 (MQ=255)
                                                                                                                                   aaaGCCGGTAACATCATTAAAGCATGGGGCGGTATCGCCGGTCTGCAAAGCTGCATGGACGTGATGTTCGATGAAGCGGTACAGAAACGCGGTATGTCTCTGCCAATGTTCGGCAAATTAATGGCGACTAACGCAGCAGATATTTTCgg                   >  1:692904/1‑149 (MQ=255)
                                                                                                                                   aaaGCCGGTAACATCATGAAAGCATGGGGCGGTATCGCCGGTCTGCAAAGCTGCATGGACGTGATGTTCGATGAAGCGGTACAGAAACGCGGTATGTCTCTGCCAATGTTCGGCAAATTAATGGCGACTAACGCAGCAGATATTTTCgg                   >  2:3625708/1‑149 (MQ=255)
                                                                                                                                    aaGCCGGTAACATCATGAAAGCATGGGGCGGTATCGCCGGTCTGCAAAGCTGCATGGACGTGATGTTCGATGAAGCGGTACAGAAACGCGGTATGTCTCTGCCAATGTTCGGCAAATTAATGGCGACTAACGCAGCAGATATTTTCGGt                  <  1:634235/149‑1 (MQ=255)
                                                                                                                                      gCCGGTAACATCATGAAAGCATGGGGCGGTATCGCCGGTCTGCAAAGCTGCATGGACGTGATGTTCGATGAAGCGGTACAGAAACGCGGTATGTCTCTGCCAATGTTCGGCAAATTAATGGCGACTAACGCAGCAGATATTTTCGGTCt                >  2:5238781/1‑149 (MQ=255)
                                                                                                                                        cGGTAACATCATGAAAGCATGGGGCGGTATCGCCGGTCTGCAAAGCTGCATGGACGTGATGTTCGATGAAGCGGTACAGAAACGCGGTATGTCTCTGCCAATGTTCGGCAAATTAATGGCGACTAACGCAGCAGATATTTTCGGTCTgc              <  1:2905758/149‑1 (MQ=255)
                                                                                                                                          gTAACATCATGAAAGCATGGGGCGGTATCGCCGGTCTGCAAAGCTGCATGGACGTGATGTTCGATGAAGCGGTACAGAAACGCGGTATGTCTCTGCCAATGTTCGGCAAATTAATGGCGACTAACGCAGCAGATATTTTCGGTCTgcag            >  1:1796542/1‑149 (MQ=255)
                                                                                                                                           tAACATCATGAAAGCATGGGGCGGTATCGCCGGTCTGCAAAGCTGCATGGACGTGATGTTCGATGAAGCGGTACAGAAACGCGGTATGTCTCTGCCAATGTTCGGCAAATTAATGGCGACTAACGCAGCAGATATTTTCGGTCTgcagc           >  2:509083/1‑149 (MQ=255)
                                                                                                                                           tAACATCATGAAAGCATGGGGCGGTATCGCCGGTCTGCAAAGCTGCATGGACGTGATGTTCGATGAAGCGGTACAGAAACGCGGTATGTCTCTGCCAATGTTCGGCAAATTAATGGCGACTAACGCAGCAGATATTTTCGGTCTgcagc           >  2:509193/1‑149 (MQ=255)
                                                                                                                                            aaCATCATGAAAGCATGGGGCGGTATCGCCGGTCTGCAAAGCTGCATGGACGTGATGTTCGATGAAGCGGTACAGAAACGCGGTATGTCTCTGCCAATGTTCGGCAAATTAATGGCGACTAACGCAGCAGATATTTTCGGTCTgcagca          >  2:809550/1‑149 (MQ=255)
                                                                                                                                                tcatGAAAGCATGGGGCGGTATCGCCGGTCTGCAAAGCTGCATGGACGTGATGTTCGATGAAGCGGTACAGAAACGCGGTATGTCTCTGCCAATGTTCGGCAAATTAATGGCGACTAACGCAGCAGATATTTTCGGTCTGCAGCaaaaa      <  2:4445308/149‑1 (MQ=255)
                                                                                                                                                    gAAAGCATGGGGCGGTATCGCCGGTCTGCAAAGCTGCATGGACGTGATGTTCGATGAAGCGGTACAGAAACGCGGTATGTCTCTGCCAATGTTCGGCAAATTAATGGCGACTAACGCAGCAGATATTTTCGGTCTGCAGCAAAAAGGcc  <  2:2630878/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
AAATCGGTACTCTGGCGAAGTGTTCACCGCCGATCCGCGATCTGGAAAACCAGAAAGGCATGTGGGAAAAACTGTTTAACGGTGAAATCGACTGCCTGGTTTCCGACCACTCTCCATGCCCGCCGGAAATGAAAGCCGGTAACATCATGAAAGCATGGGGCGGTATCGCCGGTCTGCAAAGCTGCATGGACGTGATGTTCGATGAAGCGGTACAGAAACGCGGTATGTCTCTGCCAATGTTCGGCAAATTAATGGCGACTAACGCAGCAGATATTTTCGGTCTGCAGCAAAAAGGCC  >  CP009273/535441‑535737

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑