BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	601,154	T→A	8.2%	L63F (TTA→TTT)	ybdF ←	DUF419 family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	601,154	0	T	A	8.2%	906.1 / 54.7	256	L63F (TTA→TTT)	ybdF	DUF419 family protein
Reads supporting (aligned to +/- strand): ref base T (142/93); new base A (14/7); total (156/100)
Fisher's exact test for biased strand distribution p-value = 6.47e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01

Only 100 of 263 total aligned reads displayed.

TAGCCAGACCATCAACCACCAGATTCCACGAATCGTTGATCAGATCGCGAAGTAACGCTTCGGAGATTTCTTCGCCGGGATACACTGAAATCCAGTGCTTTTTATTCATGTGATACCCTGGCTTAATGCTTGGGTATATTTGCTGATTTAACAGGGATTTTTGTGGATCGGACTTCAGATTGATAAAGGGGACGCCGCGTAGCTCCGACGACAGCATAAAAATCTTGCCGCCAATTTTAAAAACATCGAACTCCGGGCCAAAAGGCCAGCAAAGCTCGACAAAGGGTAACTCAAGG  >  CP009273/601006‑601301

 aGCCAGACCATCAACCACCAGATTCCACGAATCGTTGATCAGATCGCGAAGTAACGCTTCGGAGATTTCTTCGCCGGGATACACTGAAATCCAGTGCTTTTTATTCATGTGATACCCTGGCTTAATGCTTGGGTATATTTGCTGATTTa                                                                                                                                                    >  2:4237580/1‑149 (MQ=255)

  gCCAGACCATCAACCACCAGATTCCACGAATCGTTGATCAGATCGCGAAGTAACGCTTCGGAGATTTCTTCGCCGGGATACACTGAAATCCAGTGCTTTTTATTCATGTGATACCCTGGCTTAATGCTTGGGTATATTTGCTGATTTaa                                                                                                                                                   >  2:3202331/1‑149 (MQ=255)

    cAGACCATCAACCACCAGATTCCACGAATCGTTGATCAGATCGCGAAGTAACGCTTCGGAGATTTCGTCGCCGGGATACACTGAAATCCAGTGCTTTTTATTCATGTGATACCCTGGCTTAATGCTTGGGTATATTTGCTGATTTAACa                                                                                                                                                 <  1:5048835/149‑1 (MQ=255)

      gACCATCAACCACCAGATTCCACGAATCGTTGATCAGATCGCGAAGTAACGCTTCGGAGATTTCTTCGCCGGGATACACTGAAATCCAGTGCTTTTTATTCATGTGATACCCTGGCTTAATGCTTGGGTATATTTGCTGATTTAACAgg                                                                                                                                               <  1:318402/149‑1 (MQ=255)

      gACCATCAACCACCAGATTCCACGAATCGTTGATCAGATCGCGAAGTAACGCTTCGGAGATTTCTTCGCCGGGATACACTGAAATCCAGTGCTTTTTATTCATGTGATACCCTGGCTTAATGCTTGGGTATATTTGCTGATTTAACAgg                                                                                                                                               >  1:2849234/1‑149 (MQ=255)

      gACCATCAACCACCAGATTCCACGAATCGTTGATCAGATCGCGAAGTAACGCTTCGGAGATTTCTTCGCCGGGATACACTGAAATCCAGTGCTTTTTATTCATGTGATACCCTGGCTTAATGCTTGGGTATATTTGCTGATTTAACAgg                                                                                                                                               >  2:4411406/1‑149 (MQ=255)

      gACCATCAACCACCAGATTCCACGAATCGTTGATCAGATCGCGAAGTAACGCTTCGGAGATTTCTTCGCCGGGATACACTGAAATCCAGTGCTTTTTATTCATGTGATACCCTGGCTTAATGCTTGGGTATATTTGCTGATTTAACAgg                                                                                                                                               >  2:4411660/1‑149 (MQ=255)

      gACCATCAACCACCAGATTCCACGAATCGTTGATCAGATCGCGAAGTAACGCTTCGGAGATTTCTTCGCCGGGATACACTGAAATCCAGTGCTTTTTATTCATGTGATACCCTGGCTTAATGCTTGGGTATATTTGCTGATTAAACAgg                                                                                                                                               <  2:5194255/149‑1 (MQ=255)

         cATCAACCACCAGATTCCACGAATCGTTGATCAGATCGCGAAGTAACGCTTCGGAGATTTCTTCGCCGGGATACACTGAAATCCAGTGCTTTTTATTCATGTGATACCCTGGCTTAATGCTTGGGTATATTTGCTGATTTAACAGGGAt                                                                                                                                            >  2:3557663/1‑149 (MQ=255)

         cATCAACCACCAGATTCCACGAATCGTTGATCAGATCGCGAAGTAACGCTTCGGAGATTTCTTCGCCGGGATACACTGAAATCCAGTGCTTTTTATTCATGTGATACCCTGGCTTAATGCTTGGGTATATTTGCTGATTTAACAGGGAt                                                                                                                                            >  1:2654602/1‑149 (MQ=255)

            cAACCACCAGATTCCACGAATCGTTGATCAGATCGCGAAGTAACGCTTCGGAGATTTCTTCGCCGGGATACACTGAAATCCAGTGCTTTTTATTCATGTGATACCCTGGCTTAATGCTTGGGTATATTTGCTGATTTAACAGGGAtttt                                                                                                                                         >  1:3040725/1‑149 (MQ=255)

            cAACCACCAGATTCCACGAATCGTTGATCAGATCGCGAAGTAACGCTTCGGAGATTTCTTCGCCGGGATACACTGAAATCCAGTGCTTTTTATTCATGTGATACCCTGGCTTAATGCTTGGGTATATTTGCTGATTTAACAGGGAtttt                                                                                                                                         >  2:1141283/1‑149 (MQ=255)

             aaCCACCAGATTCCACGAATCGTTGATCAGATCGCGAAGTAACGCTTCGGAGATTTCTTCGCCGGGATACACTGAAATCCAGTGCTTTTTATTCATGTGATACCCTGGCTTAATGCTTGGGTATATTTGCTGATTTAACAGGGAttttt                                                                                                                                        <  2:4619172/149‑1 (MQ=255)

                caccaGATTCCACGAATCGTTGATCAGATCGCGAAGTAACGCTTCGGAGATTTCTTCGCCGGGATACACTGAAATCCAGTGCTTTTTATTCATGTGATACCCTGGCTTAATGCTTGGGTATATTTGCTGATTTAACAGGGATTTTtgtg                                                                                                                                     <  2:2515960/149‑1 (MQ=255)

                 accaGATTCCACGAATCGTTGATCAGATCGCGAAGTAACGCTTCGGAGATTTCTTCGCCGGGATACACTGAAATCCAGTGCTTTTTATTCATGTGATACCCTGGCTTAATGCTTGGGTATATTTGCTGATTTAACAGGGATTTTTGTgg                                                                                                                                    >  1:2940775/1‑149 (MQ=255)

                 accaGATTCCACGAATCGTTGATCAGATCGCGAAGTAACGCTTCGGAGATTTCTTCGCCGGGATACACTGAAATCCAGTGCTTTTTATTCATGTGATACCCTGGCTTAATGCTTGGGTATATTTGCTGATTTAACAGGGATTTTTGTgg                                                                                                                                    >  1:1657037/1‑149 (MQ=255)

                   caGATTCCACGAATCGTTGATCAGATCGCGAAGTAACGCTTCGGAGATTTCTTCGCCGGGATACACTGAAATCCAGTGCTTTTTATTCATGTGATACCCTGGCTTAATGCTTGGGTATATTTGCTGATTTAACAGGGATTTTTGTGGAt                                                                                                                                  >  2:5000737/1‑149 (MQ=255)

                     gATTCCACGAATCGTTGATCAGATCGCGAAGTAACGCTTCGGAGATTTCTTCGCCGGGATACACTGAAATCCAGTGCTTTTTATTCATGTGATACCCTGGCTTAATGCTTGGGTATATTTGCTGATTTAACAGGGATTTTTGTGGATCg                                                                                                                                >  2:4283266/1‑149 (MQ=255)

                      aTTCCACGAATCGTTGATCAGATCGCGAAGTAACGCTTCGGAGATTTCTTCGCCGGGATACACTGAAATCCAGTGCTTTTTATTCATGTGATACCCTGGCTTAATGCTTGGGTATATTTGCTGATTTAACAGGGATTTTTGTGGATCgg                                                                                                                               >  1:3946753/1‑149 (MQ=255)

                        tCCACGAATCGTTGATCAGATCGCGAAGTAACGCTTCGGAGATTTCTTCGCCGGGATACACTGAAATCCAGTGCTTTTTATTCATGTGATACCCTGGCTTAATGCTTGGGTATATTTGCTGATTTAACAGGGATTTTTGTGGATCGGAc                                                                                                                             >  1:1753578/1‑149 (MQ=255)

                           aCGAATCGTTGATCAGATAGCGAAGTAAAGCTTCGGAGATTTCTTCGCAGGGATACACTGAAATCAAGTGCTTTTTATTCATGTGATACCCTGTCTTAATGCATGGGTATATTTGATGATTTAACAGTGATTTTTGTGTATAGGACtac                                                                                                                          >  1:3833098/1‑147 (MQ=255)

                              aaTCGTTGATCAGATCGCGAAGTAACGCTTCGGAGATTTCTTCGCCGGGATACACTGAAATCCAGTGCTTTTTATTCATGTGATACCCTGGCTTAATGCTTGGGTATATTTGCTGATTTAACAGGGATTTTTGTGGATCGGACTTCAGa                                                                                                                       >  2:436017/1‑149 (MQ=255)

                                  gTTGATCAGATCGCGAAGTAACGCTTCGGAGATTTCTTCGCCGGGATACACTGAAATCCAGTGCTTTTTATTCATGTGATACCCTGGCTTAATGCTTGGGTATATTTGCTGATTAAACAGGGATTTTTGTGGATCGGACTTCAGATTGa                                                                                                                   <  2:3027958/149‑1 (MQ=255)

                                      aTCAGATCGCGAAGTAACGCTTCGGAGATTTCTTCGCCGGGATACACTGAAATCCAGTGCTTTTTATTCATGTGATACCCTGGCTTAATGCTTGGGTATATTTGCTGATTTAACAGGGATTTTTGTGGATCGGACTTCAGATTGATaaa                                                                                                               <  1:2725386/149‑1 (MQ=255)

                                        cAGATCGCGAAGTAACGCTTCGGAGATTTCTTCGCCGGGATACACTGAAATCCAGTGCTTTTTATTCATGTGATACCCTGGCTTAATGCTTGGGTATATTTGCTGATTTAACAGGGATTTTTGTGGATCGGACTTCAGATTGATAAAgg                                                                                                             >  1:771353/1‑149 (MQ=255)

                                         aGATCGCGAAGTAACGCTTCGGAGATTTCTTCGCCGGGATACACTGAAATCCAGTGCTTTTTATTCATGTGATACCCTGGCTTAATGCTTGGGTATATTTGCTGATTTAACAGGGATTTTTGTGGATCGGACTTCAGATTGATAAAggg                                                                                                            >  2:1112496/1‑149 (MQ=255)

                                           aTCGCGAAGTAACGCTTCGGAGATTTCTTCGCCGGGATACACTGAAATCCAGTGCTTTTTATTCATGTGATACCCTGGCTTAATGCTTGGGTATATTTGCTGATTAAACAGGGATTTTTGTGGATCGGACTTCAGATTGATAAAGGGGa                                                                                                          <  2:2688198/149‑1 (MQ=255)

                                              gcgAAGTAACGCTTCGGAGATTTCTTCGCCGGGATACACTGAAATCCAGTGCTTTTTATTCATGTGATACCCTGGCTTAATGCTTGGGTATATTTGCTGATTTAACAGGGATTTTTGTGGATCGGACTTCAGATTGATAAAGGGGAcgc                                                                                                       <  2:4057662/149‑1 (MQ=255)

                                                 aaGTAACGCTTCGGAGATTTCTTCGCCGGGATACACTGAAATCCAGTGCTTTTTATTCATGTGATACCCTGGCTTAATGCTTGGGTATATTTGCTGATTTAACAGGGATTTTTGTGGATCGGACTTCAGATTGATAAAGGGGAcgccgc                                                                                                    <  1:3349762/149‑1 (MQ=255)

                                                  aGTAACGCTTCGGAGATTTCTTCGCCGGGATACACTGAAATCCAGTGCTTTTTATTCATGTGATACCCTGGCTTAATGCTTGGGTATATTTGCTGATTTAACAGGGATTTTTGTGGATCGGACTTCAGATTGATAAAGGGGACGCcgcg                                                                                                   >  1:4336269/1‑149 (MQ=255)

                                                    tAACGCTTCGGAGATTTCTTCGCCGGGATACACTGAAATCCAGTGCTTTTTATTCATGTGATACCCTGGCTTAATGCTTGGGTATATTTGCTGATTTAACAGGGATTTTTGTGGATCGGACTTCAGATTGATAAAGGGGACGCCGCGTa                                                                                                 >  1:102633/1‑149 (MQ=255)

                                                     aaCGCTTCGGAGATTTCTTCGCCGGGATACACTGAAATCCAGTGCTTTTTATTCATGTGATACCCTGGCTTAATGCTTGGGTATATTTGCTGATTTAACAGGGATTTTTGTGGATCGGACTTCAGATTGATAAAGGGGACGCCGCGTAg                                                                                                >  1:609125/1‑149 (MQ=255)

                                                     aaCGCTTCGGAGATTTCTTCGCCGGGATACACTGAAATCCAGTGCTTTTTATTCATGTGATACCCTGGCTTAATGCTTGGGTATATTTGCTGATTTAACAGGGATTTTTGTGGATCGGACTTCAGATTGATAAAGGGGACGCCGCGTAg                                                                                                >  1:4625247/1‑149 (MQ=255)

                                                      aCGCTTCGGAGATTTCTTCGCCGGGATACACTGAAATCCAGTGCTTTTTATTCATGTGATACCCTGGCTTAATGCTTGGGTATATTTGCTGATTTAACAGGGATTTTTGTGGATCGGACTTCAGATTGATAAAGGGGACGCCGCGTAGc                                                                                               <  2:3853317/149‑1 (MQ=255)

                                                      aCGCTTCGGAGATTTCTTCGCCGGGATACACTGAAATCCAGTGCTTTTTATTCATGTGATACCCTGGCTTAATGCTTGGGTATATTTGCTGATTTAACAGGGATTTTTGTGGATCGGACTTCAGATTGAGAAAGGGGACGCCGCGTAGc                                                                                               >  2:2546753/1‑149 (MQ=255)

                                                        gCTTCGGAGATTTCTTCGCCGGGATACACTGAAATCCAGTGCTTTTTATTCATGTGATACCCTGGCTTAATGCTTGGGTATATTTGCTGATTTAACAGGGATTTTTGTGGATCGGACTTCAGATTGATAAAGGGGACGCCGCGTAGCTc                                                                                             <  2:1311528/149‑1 (MQ=255)

                                                           tCGGAGATTTCTTCGCCGGGATACACTGAAATCCAGTGCTTTTTATTCATGTGATACCCTGGCTTAATGCTTGGGTATATTTGCTGATTTAACAGGGATTTTTGTGGATCGGACTTCAGATTGATAAAGGGGACGCCGCGTAGCTCcga                                                                                          >  2:4072013/1‑149 (MQ=255)

                                                                      ttcGCCGGGATACACTGAAATCCAGTGCTTTTTATTCATGTGATACCCTGGCTTAATGCTTGGGTATATTTGCTGATTTAACAGGGATTTTTGTGGATCGGACTTCAGATTGATAAAGGGGACGCCGCGTAGCTCCGACGACAGCATaa                                                                               <  1:1101124/149‑1 (MQ=255)

                                                                         gCCGGGATACACTGGAATCCAGTGCTTTTTATTCATGTGATACCCTGGCTTAATGCTTGGGTATATTTGCTGATTTAACAGGGATTTTTGTGGATCGGACTTCAGATTGATAAAGGGGACGCCGCGTAGCTCCGACGACAGCATaaaaa                                                                            <  1:1921348/149‑1 (MQ=255)

                                                                          ccGGGATACACTGAAATCCAGTGCTTTTTATTCATGTGATACCCTGGCTTAATGCTTGGGTATATTTGCTGATTTAACAGGGATTTTTGTGGATCGGACTTCAGATTGATAAAGGGGACGCCGCGTAGCTCCGACGACAGCATAAAAAt                                                                           >  1:2968480/1‑149 (MQ=255)

                                                                            gggATACACTGAAATCCAGTGCTTTTTATTCATGTGATACCCTGGCTTAATGCTTGGGTATATTTGCTGATTTAACAGGGATTTTTGTGGATCGGACTTCAGATTGATAAAGGGGACGCCGCGTAGCTCCGACGACAGCATAAAAATCt                                                                         >  1:3378537/1‑149 (MQ=255)

                                                                            gggATACACTGAAATCCAGTGCTTTTTATTCATGTGATACCCTGGCTTAATGCTTGGGTATATTTGCTGATTTAACAGGGATTTTTGTGGATCGGACTTCAGATTGATAAAGGGGACGCCGCGTAGCTCCGACGACAGCATAAAAATCt                                                                         <  2:1955152/149‑1 (MQ=255)

                                                                            gggATACACTGAAATCCAGTGCTTTTTATTCATGTGATACCCTGGATTAATGCTTGGGTATATTTGCTGATTTAACAGGGATTTTTGTGGATCGGACTTCAGAGTGATAAAGGGGACGCCGCGTAGCTCCGACGACAGCATAAAAATCt                                                                         >  1:4850207/1‑149 (MQ=255)

                                                                             ggATACACTGAAATCCAGTGCTTTTTATTCATGTGATACCCTGGCTTAATGCTTGGGTATATTTGCTGATTAAACAGGGATTTTTGTGGATCGGACTTCAGATTGATAAAGGGGACGCCGCGTAGCTCCGACGACAGCATAAAAATCtt                                                                        >  2:671473/1‑149 (MQ=255)

                                                                               aTACACTGAAATCCAGTGCTTTTTATTCATGTGATACCCTGGCTTAATGCTTGGGTATATTTGCTGATTTAACAGGGATTTTTGTGGATCGGACTTCAGATTGATAAAGGGGACGCCGCGTAGCTCCGACGACAGCATAAAAATCTTgc                                                                      >  1:4245071/1‑149 (MQ=255)

                                                                                 acacTGAAATCCAGTGCTTTTTATTCATGTGATACCCTGGCTTAATGCTTGGGTATATTTGCTGATTTAACAGGGATTTTTGTGGATCGGACTTCAGATTGATAAAGGGGACGCCGCGTAGCTCCGACGACAGCATAAAAATCTTgccg                                                                    >  1:2125633/1‑149 (MQ=255)

                                                                                   acTGAAATCCAGTGCTTTTTATTCATGTGATACCCTGGCTTAATGCTTGGGTATATTTGCTGATTTAACAGGGATTTTTGTGGATCGGACTTCAGATTGATAAAGGGGACGCCGCGTAGCTCCGACGACAGCATAAAAATCTTgccgcc                                                                  >  1:3754809/1‑149 (MQ=255)

                                                                                      gAAATCCAGTGCTTTTTATTCATGTGATACCCTGGCTTAATGCTTGGGTATATTTGCTGATTTAACAGGGATTTTTGTGGATCGGACTTCAGATTGATAAAGGGGACGCCGCGTAGCTCCGACGACAGCATAAAAATCTTGCCGCCAAt                                                               <  2:3452075/149‑1 (MQ=255)

                                                                                       aaaTCCAGTGCTTTTTATTCATGTGATACCCTGGCTTAATGCTTGGGTATATTTGCTGATTTAACAGGGATTTTTGTGGATCGGACTTCAGATTGATAAAGGGGACGCCGCGTAGCTCCGACGACAGCATAAAAATCTTGCCGCCAAtt                                                              <  1:4169650/149‑1 (MQ=255)

                                                                                       aaaTCCAGTGCTTTTTATTCATGTGATACCCTGGCTTAATGCTTGGGTATATTTGCTGATTTAACAGGGATTTTTGTGGATCGGACTTCAGATTGATAAAGGGGACGCCGCGTAGCTCCGACGACAGCATAAAAATCTTGCCGCCAAtt                                                              >  1:1995768/1‑149 (MQ=255)

                                                                                        aaTCCAGTGCTTTTTATTCATGTGATACCCTGGCTTAATGCTTGGGTATATTTGCTGATTTAACAGGGATTTTTGTGGATCGGACTTCAGATTGATAAAGGGGACGCCGCGTAGCTCCGACGACAGCATAAAAATCTTGCCGCCAAttt                                                             >  2:282133/1‑149 (MQ=255)

                                                                                        aaTCCAGTGCTTTTTATTCATGTGATACCCTGGCTTAATGCTTGGGTATATTTGCTGATTTAACAGGGATTTTTGTGGATCGGACTTCAGATTGATAAAGGGGACGCCGCGTAGCTCCGACGACAGCATAAAAATCTTGCCGCCAAttt                                                             >  2:3436690/1‑149 (MQ=255)

                                                                                        aaTCCAGTGCTTTTTATTCATGTGATACCCTGGCTTAATGCTTGGGTATATTTGCTGATTTAACAGGGATTTTTGTGGATCGGACTTCAGATTGATAAAGGGGACGCCGCGTAGCTCCGACGACAGCATAAAAATCTTGCCGCCAAttt                                                             >  1:1905563/1‑149 (MQ=255)

                                                                                          tCCAGTGCTTTTTATTCATGTGATACCCTGGCTTAATGCTTGGGTATATTTGCTGATTTAACAGGGATTTTTGTGGATCGGACTTCAGATTGATAAAGGGGACGCCGCGTAGCTCCGACGACAGCATAAAAATCTTGCCGCCAATTTTa                                                           >  2:3024654/1‑149 (MQ=255)

                                                                                          tCCAGTGCTTTTTATTCATGTGATACCCTGGCTTAATGCTTGGGTATATTTGCTGATTTAACAGGGATTTTTGTGGATCGGACTTCAGATTGATAAAGGGGACGCCGCGTAGCTCCGACGACAGCATAAAAATCTTGCCGCCAATTTTa                                                           >  2:3915155/1‑149 (MQ=255)

                                                                                           ccAGTGCTTTTTATTCATGTGATACCCTGGCTTAATGCTTGGGTATATTTGCTGATTTAACAGGGATTTTTGTGGATCGGACTTCAGATTGATAAAGGGGACGCCGCGTAGCTCCGACGACAGCATAAAAATCTTGCCGCCAATTTTaa                                                          >  2:761972/1‑149 (MQ=255)

                                                                                             aGTGCTTTTTATTCATGTGATACCCTGGCTTAATGCTTGGGTATATTTGCTGATTTAACAGGGATTTTTGTGGATCGGACTTCAGATTGATAAAGGGGACGCCGCGTAGCTCCGACGACAGCATAAAAATCTTGCCGCCAATTTTaaaa                                                        <  2:612654/149‑1 (MQ=255)

                                                                                              gTGCTTTTTATTCATGTGATACCCTGGCTTAATGCTTGGGTATATTTGCTGATTTAACAGGGATTTTTGTGGATCGGACTTCAGATTGATAAAGGGGACGCCGCGTAGCTCCGACGACAGCATAAAAATCTTGCCGCCAATTTTaaaaa                                                       >  1:990469/1‑149 (MQ=255)

                                                                                                 cTTTTTATTCATGTGATACCCTGGCTTAATGCTTGGGTATATTTGCTGATTTAACAGGGATTTTTGTGGATCGGACTTCAGATTGATAAAGGGGACGCCGCGTAGCTCCGACGACAGCATAAAAATCTTGCCGCCAATTTTAAAAACAt                                                    >  1:3860379/1‑149 (MQ=255)

                                                                                                 cTTTTTATTCATGTGATACCCTGGCTTAATGCTTGGGTATATTTGCTGATTTAACAGGGATTTTTGTGGATCGGACTTCAGATTGATAAAGGGGACGCCGCGTAGCTCCGACGACAGCATAAAAATCTTGCCGCCAATTTTAAAAACAt                                                    >  1:3932581/1‑149 (MQ=255)

                                                                                                  tttttATTCATGTGATACCCTGGCTTAATGCTTGGGTATATTTGCTGATTTAACAGGGATTTTTGTGGATCGGACTTCAGATTGATAAAGGGGACGCCGCGTAGCTCCGACGACAGCATAAAAATCTTGCCGCCAATTTTAAAAACATc                                                   <  1:480115/149‑1 (MQ=255)

                                                                                                  tttttATTCATGTGATACCCTGGCTTAATGCTTGGGTATATTTGCTGATTTAACAGGGATTTTTGTGGATCGGACTTCAGATTGATAAAGGGGACGCCGCGTAGCTCCGACGACAGCATAAAAATCTTGCCGCCAATTTTAAAAACATc                                                   >  1:360734/1‑149 (MQ=255)

                                                                                                  tttttATTCATGTGATACCCTGGCTTAATGCTTGGGTATATTTGCTGATTTAACAGGGATTTTTGTGGATCGGACTTCAGATTGATAAAGGGGACGCCGCGTAGCTCCGACGACAGCATAAAAATCTTGCCGCCAATTTTAAAAACATc                                                   >  1:1169403/1‑149 (MQ=255)

                                                                                                   ttttATTCATGTGATACCCTGGCTTAATGCTTGGGTATATTTGCTGATTTAACAGGGATTTTTGTGGATCGGACTTCAGATTGATAAAGGGGACGCCGCGTAGCTCCGACGACAGCATAAAAATCTTGCCGCCAATTTTAAAAACATCg                                                  <  1:5092235/149‑1 (MQ=255)

                                                                                                   ttttATTCATGTGATACCCTGGCTTAATGCTTGGGTATATTTGCTGATTAAACAGGGATTTTTGTGGATCGGACTTCAGATTGATAAAGGGGACGCCGCGTAGCTCCGACGACAGCATAAAAATCTTGCCGCCAATTTTAAAAACATCg                                                  <  1:1272120/149‑1 (MQ=255)

                                                                                                   ttttATTCATGTGATACCCTGGCTTAATGCTTGGGTATATTTGCTGATTAAACAGGGATTTTTGTGGATCGGACTTCAGATTGATAAAGGGGACGCCGCGTAGCTCCGACGACAGCATAAAAATCTTGCCGCCAATTTTAAAAACATCg                                                  >  1:4955/1‑149 (MQ=255)

                                                                                                     ttATTCATGTGATACCCTGGCTTAATGCTTGGGTATATTTGCTGATTTAACAGGGATTTTTGTGGATCGGACTTCAGATTGATAAAGGGGACGCCGCGTAGCTCCGACGACAGCATAAAAATCTTGCCGCCAATTTTAAAAACATCGaa                                                >  2:2060426/1‑149 (MQ=255)

                                                                                                       aTTCATGTGATACCCTGGCTTAATGCTTGGGTATATTTGCTGATTTAACAGGGATTTTTGTGGATCGGACTTCAGATTGATAAAGGGGACGCCGCGTAGCTCCGACGACAGCATAAAAATCTTGCCGCCAATTTTAAAAACATCGAACt                                              >  2:2703653/1‑149 (MQ=255)

                                                                                                         tCATGTGATACCCTGGCTTAATGCTTGGGTATATTTGCTGATTTAACAGGGATTTTTGTGGATCGGACTTCAGATTGATAAAGGGGACGCCGCGTAGCTCCGACGACAGCATAAAAATCTTGCCGCCAATTTTAAAAACATCGAACTcc                                            <  2:2380081/149‑1 (MQ=255)

                                                                                                         tCATGTGATACCCTGGCTTAATGCTTGGGTATATTTGCTGATTTAACAGGGATTTTTGTGGATCGGACTTCAGATTGATAAAGGGGACGCCGCGTAGCTCCGACGACAGCATAAAAATCTTGCCGCCAATTTTAAAAACATCGAACTcc                                            >  1:1588597/1‑149 (MQ=255)

                                                                                                           aTGTGATACCCTGGCTTAATGCTTGGGTATATTTGCTGATTTAACAGGGATTTTTGTGGATCGGACTTCAGATTGATAAAGGGGACGCCGCGTAGCTCCGACGACAGCATAAAAATCTTGCCGCCAATTTTAAAAACATCGAACTCCgg                                          >  1:5227185/1‑149 (MQ=255)

                                                                                                             gtgATACCCTGGCTTAATGCTTGGGTATATTTGCTGATTTAACAGGGATTTTTGTGGATCGGACTTCAGATTGATAAAGGGGACGCCGCGTAGCTCCGACGACAGCATAAAAATCTTGCCGCCAATTTTAAAAACATCGAACTCCGGGc                                        <  2:1377528/149‑1 (MQ=255)

                                                                                                              tgATACCCTGGCTTAATGCTTGGGTATATTTGCTGATTTAACAGGGATTTTTGTGGATCGGACTTCAGATTGATAAAGGGGACGCCGCGTAGCTCCGACGACAGCATAAAAATCTTGCCGCCAATTTTAAAAACATCGAACTCCGGGcc                                       <  1:4121363/149‑1 (MQ=255)

                                                                                                               gATACCCTGGCTTAATGCTTGGGTATATTTGCTGATTTAACAGGGATTTTTGTGGATCGGACTTCAGATTGATAAAGGGGACGCCGCGTAGCTCCGACGACAGCATAAAAATCTTGCCGCCAATTTTAAAAACATCGAACTCCGGGCCa                                      >  2:2294246/1‑149 (MQ=255)

                                                                                                                 tACCCTGGCTTAATGCTTGGGTATATTTGCTGATTTAACAGGGATTTTTGTGGATCGGACTTCAGATTGATAAAGGGGACGCCGCGTAGCTCCGACGACAGCATAAAAATCTTGCCGCCAATTTTAAAAACATCGAACTCCGGGCCaaa                                    >  2:16833/1‑149 (MQ=255)

                                                                                                                  aCCCTGGCTTAATGCTTGGGTATATTTGCTGATTTAACAGGGATTTTTGTGGATCGGACTTCAGATTGATAAAGGGGACGCCGCGTAGCTCCGACGACAGCATAAAAATCTTGCCGCCAATTTTAAAAACATCGAACTCCGGGCCaaaa                                   <  2:475011/149‑1 (MQ=255)

                                                                                                                   cccTGGCTTAATGCTTGGGTATATTTGCTGATTTAACAGGGATTTTTGTGGATCGGACTTCAGATTGATAAAGGGGACGCCGCGTAGCTCCGACGACAGCATAAAAATCTTGCCGCCAATTTTAAAAACATCGAACTCCGGGCCAAAAg                                  >  2:4213431/1‑149 (MQ=255)

                                                                                                                   cccTGGCTTAATGCTTGGGTATATTTGCTGATTTAACAGGGATTTTTGTGGATCGGACTTCAGATTGATAAAGGGGACGCCGCGTAGCTCCGACGACAGCATAAAAATCTTGCCGCCAATTTTAAAAACATCGAACTCCGGGCCAAAAg                                  >  2:1346645/1‑149 (MQ=255)

                                                                                                                    ccTGGCTTAATGCTTGGGTATATTTGCTGATTTAACAGGGATTTTTGTGGATCGGACTTCAGATTGATAAAGGGGACGCCGCGTAGCTCCGACGACAGCATAAAAATCTTGCCGCCAATTTTAAAAACATCGAACTCCGGGCCAAAAgg                                 >  2:20957/1‑149 (MQ=255)

                                                                                                                      tGGCTTAATGCTTGGGTATATTTGCTGATTTAACAGGGATTTTTGTGGATCGGACTTCAGATTGATAAAGGGGACGCCGCGTAGCTCCGACGACAGCATAAAAATCTTGCCGCCAATTTTAAAAACATCGAACTCCGGGCCAAAAGGcc                               >  1:56714/1‑149 (MQ=255)

                                                                                                                       ggCTTAATGCTTGGGTATATTTGCTGATTTAACAGGGATTTTTGTGGATCGGACTTCAGATTGATAAAGGGGACGCCGCGTAGCTCCGACGACAGCATAAAAATCTTGCCGCCAATTTTAAAAACATCGAACTCCGGGCCAAAAGGCCa                              <  1:4415425/149‑1 (MQ=255)

                                                                                                                            aaTGCTTGGGTATATTTGCTGATTTAACAGGGATTTTTGTGGATCGGACTTCAGATTGATAAAGGGGACGCCGCGTAGCTCCGACGACAGCATAAAAATCTTGCCGCCAATTTTAAAAACATCGAACTCCGGGCCAAAAGGCCAGCaaa                         >  1:1360709/1‑149 (MQ=255)

                                                                                                                             aTGCTTGGGTATATTTGCTGATTTAACAGGGATTTTTGTGGATCGGACTTCAGATTGATAAAGGGGACGCCGCGTAGCTCCGACGACAGCATAAAAATCTTGCCGCCAATTTTAAAAACATCGAACTCCGGGCCAAAAGGCCAGCAAAg                        <  2:2718788/149‑1 (MQ=255)

                                                                                                                              tGCTTGGGTATATTTGCTGATTTAACAGGGATTTTTGTGGATCGGACTTCAGATTGATAAAGGGGACGCCGCGTAGCTCCGACGACAGCATAAAAATCTTGCCGCCAATTTTAAAAACATCGAACTCCGGGCCAAAAGGCCAGCAAAGc                       <  2:4524475/149‑1 (MQ=255)

                                                                                                                              tGCTTGGGTATATTTGCTGATTTAACAGGGATTTTTGTGGATCGGACTTCAGATTGATAAAGGGGACGCCGCGTAGCTCCGACGACAGCATAAAAATCTTGCCGCCAATTTTAAAAACATCGAACTCCGGGCCAAAAGGCCAGCAAAGc                       >  1:2837053/1‑149 (MQ=255)

                                                                                                                                cTTGGGTATATTTGCTGATTAAACAGGGATTTTTGTGGATCGGACTTCAGATTGATAAAGGGGACGCCGCGTAGCTCCGACGACAGCATAAAAATCTTGCCGCCAATTTTAAAAACATCGAACTCCGGGCCAAAAGGCCAGCAAAGCTc                     >  2:1801245/1‑149 (MQ=255)

                                                                                                                                   gggTATATTTGCTGATTTAACAGGGATTTTTGTGGATCGGACTTCAGATTGATAAAGGGGACGCCGCGTAGCTCCGACGACAGCATAAAAATCTTGCCGCCAATTTTAAAAACATCGAACTCCGGGCCAAACGGCCAGCAAAGCTCGAc                  <  2:2958148/149‑1 (MQ=255)

                                                                                                                                   gggTATATTTGCTGATTTAACAGGGATTTTTGTGGATCGGACTTCAGATTGATAAAGGGGACGCCGCGTAGCTCCGACGACAGCATAAAAATCTTGCCGCCAATTTTAAAAACATCGAACTCCGGGCCAAAAGGCCAGCAAAGCTCGAc                  <  1:4534744/149‑1 (MQ=255)

                                                                                                                                       atatTTGCTGATTAAACAGGGATTTTTGTGGATCGGACTTCAGATTGATAAAGGGGACGCCGCGTAGCTCCGACGACAGCATAAAAATCTTGCCGCCAATTTTAAAAACATCGAACTCCGGGCCAAAAGGCCAGCAAAGCTCGACAAAg              >  1:2455229/1‑149 (MQ=255)

                                                                                                                                         atTTGCTGATTTAACAGGGATTTTTGTGGATCGGACTTCAGATTGATAAAGGGGACGCCGCGTAGCTCCGACGACAGCATAAAAATCTTGCCGCCAATTTTAAAAACATCGAACTCCGGGCCAAAAGGCCAGCAAAGCTCGACAAAggg            <  1:4411406/149‑1 (MQ=255)

                                                                                                                                         atTTGCTGATTAAACAGGGATTTTTGTGGATCGGACTTCAGATTGATAAAGGGGACGCCGCGTAGCTCCGACGACAGCATAAAAATCTTGCCGCCAATTTTAAAAACATCGAACTCCGGGCCAAAAGGCCAGCAAAGCTCGACAAAggg            >  2:3004630/1‑149 (MQ=255)

                                                                                                                                          tttGCTGATTTAACAGGGATTTTTGTGGATCGGACTTCAGATTGATAAAGGGGACGCCGCGTAGCTCCGACGACAGCATAAAAATCTTGCCGCCAATTTTAAAAACATCGAACTCCGGGCCAAAAGGCCAGCAAAGCTCGACAAAGGGt           >  1:2017177/1‑149 (MQ=255)

                                                                                                                                             gCTGATTTAACAGGGATTTTTGTGGATCGGACTTCAGATTGATAAAGGGGACGCCGCGTAGCTCCGACGACAGCATAAAAATCTTGCCGCCAATTTTAAAAACATCGAACTCCGGGCCAAAAGGCCAGCAAAGCTCGACAAAGGGTAAc        >  1:826182/1‑149 (MQ=255)

                                                                                                                                              cTGATTTAACAGGGATTTTTGTGGATCGGACTTCAGATTGATAAAGGGGACGCCGCGTAGCTCCGACGACAGCATAAAAATCTTGCCGCCAATTTTAAAAACATCGAACTCCGGGCCAAAAGGCCAGCAAAGCTCGACAAAGGGTAACt       >  1:4448393/1‑149 (MQ=255)

                                                                                                                                                gATTTAACAGGGATTTTTGTGGATCGGACTTCAGATTGATAAAGGGGACGCCGCGTAGCTCCGACGACAGCATAAAAATCTTGCCGCCAATTTTAAAAACATCGAACTCCGGGCCAAAAGGCCAGCAAAGCTCGACAAAGGGGAACTCa     >  2:3714381/1‑149 (MQ=255)

                                                                                                                                                 aTTTAACAGGGATTTTTGTGGATCGGACTTCAGATTGATAAAGGGGACGCCGCGTAGCTCCGACGACAGCATAAAAATCTTGCCGCCAATTTTAAAAACATCGAACTCCGGGCCAAAAGGCCAGCAAAGCTCGACAAAGGGTAACTCaa    >  2:3351680/1‑149 (MQ=255)

                                                                                                                                                 aTTTAACAGGGATTTTTGTGGATCGGACTTCAGATTGATAAAGGGGACGCCGCGTAGCTCCGACGACAGCATAAAAATCTTGCCGCCAATTTTAAAAACATCGAACTCCGGGCCAAAAGGCCAGCAAAGCTCGACAAAGGGTAACTCaa    >  2:4913460/1‑149 (MQ=255)

                                                                                                                                                  tttAACAGGGATTTTTGTGGATCGGACTTCAGATTGATAAAGGGGACGCCGCGTAGCTCCGAAGACAGCATAAAAATCTTGCCGCCAATTTTAAAAACATCGAACTCCGGGCCAAAAGGCCAGCAAAGCTCGACAAAGGGTAACTCAAg   <  1:3212309/149‑1 (MQ=255)

                                                                                                                                                   ttAACAGGGATTTTTGTGGATCGGACTTCAGATTGATAAAGGGGACGCCGCGTAGCTCCGACGACAGCATAAAAATCTTGCCGCCAATTTTAAAAACATCGAACTCCGGGCCAAAAGGCCAGCAAAGCTCGACAAAGGGTAACTCAAgg  >  2:2562143/1‑149 (MQ=255)

                                                                                                                                                   ttAACAGGGATTTTTGTGGATCGGACTTCAGATTGATAAAGGGGACGCCGCGTAGCTCCGACGACAGCATAAAAATCTTGCCGCCAATTTTAAAAACATCGAACTCCGGGCCAAAAGGCCAGCAAAGCTCGACAAAGGGTAACTCAAgg  <  2:2280225/149‑1 (MQ=255)

TAGCCAGACCATCAACCACCAGATTCCACGAATCGTTGATCAGATCGCGAAGTAACGCTTCGGAGATTTCTTCGCCGGGATACACTGAAATCCAGTGCTTTTTATTCATGTGATACCCTGGCTTAATGCTTGGGTATATTTGCTGATTTAACAGGGATTTTTGTGGATCGGACTTCAGATTGATAAAGGGGACGCCGCGTAGCTCCGACGACAGCATAAAAATCTTGCCGCCAATTTTAAAAACATCGAACTCCGGGCCAAAAGGCCAGCAAAGCTCGACAAAGGGTAACTCAAGG  >  CP009273/601006‑601301

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`