Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 754,572 G→T 94.7% E137D (GAG→GAT)  sucA → 2‑oxoglutarate decarboxylase, thiamine triphosphate‑binding

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273754,5720GT94.7% 926.1 / 33.5 281E137D (GAG→GAT) sucA2‑oxoglutarate decarboxylase, thiamine triphosphate‑binding
Reads supporting (aligned to +/- strand):  ref base G (8/7);  new base T (140/124);  total (150/131)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.68e-01

Only 100 of 281 total aligned reads displayed.

CAGGTTAAAGTCCTGCAGCTCATTAACGCATACCGCTTCCGTGGTCACCAGCATGCGAATCTCGATCCGCTGGGACTGTGGCAGCAAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGAGACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACGCTGGATCCAACA  >  CP009273/754426‑754718
                                                                                                                                                  |                                                                                                                                                  
cAGGTTAAAGTCCTGCAGCTCATTAACGCATACCGCTTCCGTGGTCACCAGCATGCGAATCTCGATCCGCTGGGACTGTGGCAGCAAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATAcc                                                                                                                                                 >  2:2803513/1‑150 (MQ=255)
cAGGTTAAAGTCCTGCAGCTCATTAACGCATACCGCTTCCGTGGTCACCAGCATGCGAATCTCGATCCGCTGGGACTGTGGCAGCAAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATAcc                                                                                                                                                 <  1:3812462/150‑1 (MQ=255)
 aGGTTAAAGTCCTGCAGCTCATTAACGCATACCGCTTCCGTGGTCACCAGCATGCGAATCTCGATCCGCTGGGACTGTGGCAGCAAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATACCt                                                                                                                                                <  2:1550391/150‑1 (MQ=255)
     tAAAGTCCTGCAGCTCATTAACGCATACCGCTTCCGTGGTCACCAGCATGCGAATCTCGATCCGCTGGGACTGTGGCAGCAAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATACCTTCaa                                                                                                                                            <  2:2935873/150‑1 (MQ=255)
     tAAAGTCCTGCAGCTCATTAACGCATACCGCTTCCGTGGTCACCAGCATGCGAATCTCGATCCGCTGGGACTGTGGCAGCAAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATACCTTCaa                                                                                                                                            >  1:4841086/1‑150 (MQ=255)
      aaaGTCCTGCAGCTCATTAACGCATACCGCTTCCGTGGTCACCAGCATGCGAATCTCGATCCGCTGGGACTGTGGCAGCAAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATACCTTCAAc                                                                                                                                           <  1:794616/150‑1 (MQ=255)
          tCCTGCAGCTCATTAACGCATACCGCTTCCGTGGTCACCAGCATGCGAATCTCGATCCGCTGGGACTGTGGCAGCAAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATACCTTCAACGTCg                                                                                                                                       >  2:1850403/1‑150 (MQ=255)
          tCCTGCAGCTCATTAACGCATACCGCTTCCGTGGTCACCAGCATGCGAATCTCGATCCGCTGGGACTGTGGCAGCAAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGAGACCTTCAACGTCg                                                                                                                                       <  1:1709155/150‑1 (MQ=255)
             tGCAGCTCATTAACGCATACCGCTTCCGTGGTCACCAGCATGCGAATCTCGATCCGCTGGGACTGTGGCAGCAAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATACCTTCAACGTCGGtt                                                                                                                                    <  2:2149873/150‑1 (MQ=255)
               cAGCTCATTAACGCATACCGCTTCCGTGGTCACCAGCATGCGAATCTCGATCCGCTGGGACTGTGGCAGCAAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCa                                                                                                                                  <  2:554268/150‑1 (MQ=255)
               cAGCTCATTAACGCATACCGCTTCCGTGGTCACCAGCATGCGAAACTCGATCCGCTGGGACTGTGGCAGCAAGATAAATTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATACATTCAACGTCGGTTCa                                                                                                                                  >  1:67874/1‑150 (MQ=255)
                aGCTCATTAACGCATACCGCTTCCGTGGTCACCAGCATGCGAATCTCGATCCGCTGGGACTGTGGCAGCAAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCAt                                                                                                                                 <  1:5095777/150‑1 (MQ=255)
                  cTCATTAACGCATACCGCTTCCGTGGTCACCAGCATGCGAATCTCGATCCGCTGGGACTGTGGCAGCAAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGAGACCTTCAACGTCGGTTCAttt                                                                                                                               <  1:2867378/150‑1 (MQ=255)
                   tCATTAACGCATACCGCTTCCGTGGTCACCAGCATGCGAATCTCGATCCGCTGGGACTGTGGCAGCAAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTg                                                                                                                              <  2:4760661/150‑1 (MQ=255)
                    cATTAACGCATACCGCTTCCGTGGTCACCAGCATGCGAATCTCGATCCGCTGGGACTGTGGCAGCAAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGAGACCTTCAACGTCGGTTCATTTGc                                                                                                                             >  1:4374765/1‑150 (MQ=255)
                     aTTAACGCATACCGCTTCCGTGGTCACCAGCATGCGAATCTCGATCCGCTGGGACTGTGGCAGCAAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGcc                                                                                                                            <  1:171655/150‑1 (MQ=255)
                             aTACCGCTTCCGTGGTCACCAGCATGCGAATCTCGATCCGCTGGGACTGTGGCAGCAAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCaa                                                                                                                    <  2:4298351/150‑1 (MQ=255)
                                  gCTTCCGTGGTCACCAGCATGCGAATCTCGATCCGCTGGGACTGTGGCAGCAAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGaaa                                                                                                               >  1:2986478/1‑150 (MQ=255)
                                    ttCCGTGGTCACCCGCATGCGAATCTCGATCCGCTGGGACTGTGGCAGCAAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAAcc                                                                                                             >  2:1413731/1‑150 (MQ=255)
                                    ttCCGTGGTCACCAGCATGCGAATCTCGATCCGCTGGGACTGTGGCAGCAAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAAcc                                                                                                             >  2:4412536/1‑150 (MQ=255)
                                    ttCCGTGGTCACCAGCATGCGAATCTCGATCCGCTGGGACTGTGGCAGCAAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAAcc                                                                                                             <  1:2512065/150‑1 (MQ=255)
                                     tCCGTGGTCACCAGCATGCGAATCTCGATCCGCTGGGACTGTGGCAGCAAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCa                                                                                                            >  1:1161887/1‑150 (MQ=255)
                                     tCCGTGGTCACCAGCATGCGAATCTCGATCCGCTGGGACTGTGGCAGCAAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGAAACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCa                                                                                                            >  1:3759446/1‑150 (MQ=255)
                                      ccGTGGTCACCAGCATGCGAATCTCGATCCGCTGGGACTGTGGCAGCAAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCAt                                                                                                           <  2:3123933/150‑1 (MQ=255)
                                        atGGTCACCAGCATGCGAATCTCGATCCGCTGGGACTGTGGCAGCAATATAAAGTGGCCGATCTGGATCCGTCTTTCCAAGATCTGACCGAAGCAGAATTCCAGGATAACTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGa                                                                                                         >  1:1329657/2‑150 (MQ=255)
                                         tGGTCACCAGCATGCGAATCTCGATCCGCTGGGACTGTGGCAGCAAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGaa                                                                                                        >  2:2656447/1‑150 (MQ=255)
                                          ggTCACCAGCATGCGAATCTCGATCCGCTGGGACTGTGGCAGCAAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGaaa                                                                                                       >  1:3651538/1‑150 (MQ=255)
                                            tCACCAGCATGCGAATCTCGATCCGCTGGGACTGTGGCAGCAAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACt                                                                                                     <  2:2572025/150‑1 (MQ=255)
                                             cACCAGCATGCGAATCTCGATCCGCTGGGACTGTGGCAGCAAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTc                                                                                                    >  2:2284405/1‑150 (MQ=255)
                                              aCCAGCATGCGAATCTCGATCCGCTGGGACTGTGGCAGCAAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCg                                                                                                   <  2:1744916/150‑1 (MQ=255)
                                              aCCAGCATGCGAATCTCGATCCGCTGGGACTGTGGCAGCAAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCg                                                                                                   >  2:1277930/1‑150 (MQ=255)
                                                cAGCATGCGAATCTCGATCCGCTGGGACTGTGGCAGCAAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGc                                                                                                 <  2:3533957/150‑1 (MQ=255)
                                                  gCATGCGAATCTCGATCCGCTGGGACTGTGGCAGCAAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGAGACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGa                                                                                               >  2:4887494/1‑150 (MQ=255)
                                                  gCATGCGAATCTCGATCCGCTGGGACTGTGGCAGCAAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGAGACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGa                                                                                               >  2:2305038/1‑150 (MQ=255)
                                                    aTGCGAATCTCGATCCGCTGGGACTGTGGCAGCAAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAgc                                                                                             <  1:3110798/150‑1 (MQ=255)
                                                    aTGCGAATCTCGATCCGCTGGGACTGTGGCAGCAAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAgc                                                                                             >  1:4026079/1‑150 (MQ=255)
                                                      gCGAATCTCGATCCGCTGGGACTGTGGCAGCAAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAgctg                                                                                           <  1:663924/150‑1 (MQ=255)
                                                       cGAATCTCGATCCGCTGGGACTGTGGCAGCAAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAgctgc                                                                                          <  2:32831/150‑1 (MQ=255)
                                                               gATCCGCTGGGACTGTGGCAGCAAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGcc                                                                                  >  1:1186655/1‑150 (MQ=255)
                                                               gATCCGCTGGGACTGTGGCAGCAAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGcc                                                                                  >  2:4150064/1‑150 (MQ=255)
                                                                 tCCGCTGGGACTGTGGCAGCAAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCt                                                                                <  1:2155446/150‑1 (MQ=255)
                                                                 tCCGCTGGGACTGTGGCAGCAAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCt                                                                                >  1:1067355/1‑150 (MQ=255)
                                                                  ccGCTGGGACTGTGGCAGCAAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTc                                                                               <  1:4407515/150‑1 (MQ=255)
                                                                   cGCTGGGACTGTGGCAGCAAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCa                                                                              >  1:1062279/1‑150 (MQ=255)
                                                                         gACTGTGGCAGCAAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCaaa                                                                        >  2:2602441/1‑150 (MQ=255)
                                                                          aCTGTGGCAGCAAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAAc                                                                       <  1:2674914/150‑1 (MQ=255)
                                                                          aCTGTGGCAGCAAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAAc                                                                       <  1:4132032/150‑1 (MQ=255)
                                                                          aCTGTGGCAGCAAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAAc                                                                       <  1:905571/150‑1 (MQ=255)
                                                                            tgtgGCAGCAAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCt                                                                     >  2:4128757/1‑150 (MQ=255)
                                                                             gtgGCAGCAAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTa                                                                    >  2:403491/1‑150 (MQ=255)
                                                                             gtgGCAGCAAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTa                                                                    <  2:2096689/150‑1 (MQ=255)
                                                                                gcagcaAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTg                                                                 <  2:513441/150‑1 (MQ=255)
                                                                                 cagcaAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGc                                                                <  1:1555547/150‑1 (MQ=255)
                                                                                  agcaAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCg                                                               >  2:533107/1‑150 (MQ=255)
                                                                                   gcaAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCgg                                                              <  1:2731193/150‑1 (MQ=255)
                                                                                       gATAAAGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCg                                                          >  1:1415814/1‑150 (MQ=255)
                                                                                        aTAAAGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGa                                                         <  1:1699669/150‑1 (MQ=255)
                                                                                        aTAAAGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGa                                                         <  1:2035349/150‑1 (MQ=255)
                                                                                             gTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGt                                                    <  2:872642/150‑1 (MQ=255)
                                                                                             gTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGt                                                    <  1:225560/150‑1 (MQ=255)
                                                                                              tGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTg                                                   <  2:1595740/150‑1 (MQ=255)
                                                                                              tGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTg                                                   <  2:2426913/150‑1 (MQ=255)
                                                                                                 ccGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCg                                                <  1:2415362/150‑1 (MQ=255)
                                                                                                   gATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAg                                              >  1:3161879/1‑150 (MQ=255)
                                                                                                   gATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGAGACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAg                                              <  2:1281058/150‑1 (MQ=255)
                                                                                                    aTCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGt                                             >  1:1327225/1‑150 (MQ=255)
                                                                                                     tCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGta                                            >  1:5067170/1‑150 (MQ=255)
                                                                                                     tCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGta                                            >  1:2858930/1‑150 (MQ=255)
                                                                                                       tGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGtata                                          <  1:2387928/150‑1 (MQ=255)
                                                                                                        ggccccGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTatat                                         <  1:95395/146‑1 (MQ=255)
                                                                                                        ggATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTatat                                         >  1:4783008/1‑150 (MQ=255)
                                                                                                           tCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGca                                      <  2:3679981/150‑1 (MQ=255)
                                                                                                                  ttCCACGATCTGACCGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTAcc                               >  1:4504359/1‑150 (MQ=255)
                                                                                                                  ttCCACGATCTGACCGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTAcc                               <  2:3372801/150‑1 (MQ=255)
                                                                                                                    ccACGATCTGACCGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAg                             <  2:171201/150‑1 (MQ=255)
                                                                                                                     cACGATCTGACCGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGc                            <  2:760369/150‑1 (MQ=255)
                                                                                                                       cGATCTGACCGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCAc                          <  1:551569/150‑1 (MQ=255)
                                                                                                                        gATCTGACCGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCAcc                         >  2:3891861/1‑150 (MQ=255)
                                                                                                                            tGACCGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCgaag                     >  1:194187/1‑150 (MQ=255)
                                                                                                                            tGACCGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCgaag                     >  2:3783510/1‑150 (MQ=255)
                                                                                                                             gACCGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCgaaga                    >  2:4258011/1‑150 (MQ=255)
                                                                                                                             gACCGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCgaaga                    >  2:516164/1‑150 (MQ=255)
                                                                                                                             gACCGAAGCAGACTTCCAGGAGACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCgaaga                    >  2:2045458/1‑150 (MQ=255)
                                                                                                                             gACCGAAGCAGACTTCCAGGAAACCTTCAACGTCGGTTCATTTGCCACCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGCAAGCCCTCAACCAAACCAACTGCGGCCCGATAGGTGCCGAGTATATGCACATTACCAGCACCgaaga                    >  1:4540239/1‑150 (MQ=255)
                                                                                                                                cGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGaaaa                 <  2:2852761/150‑1 (MQ=255)
                                                                                                                                cGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGaaaa                 >  1:1776189/1‑150 (MQ=255)
                                                                                                                                 gAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGaaaaa                >  1:1279790/1‑150 (MQ=255)
                                                                                                                                 gAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGaaaaa                <  1:2177805/150‑1 (MQ=255)
                                                                                                                                 gAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGaaaaa                >  1:2040332/1‑150 (MQ=255)
                                                                                                                                 gAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGaaaaa                <  2:1147071/150‑1 (MQ=255)
                                                                                                                                  aaGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAAc               <  1:334894/150‑1 (MQ=255)
                                                                                                                                  aaGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAAc               >  2:4506009/1‑150 (MQ=255)
                                                                                                                                  aaGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAAc               >  1:3580229/1‑150 (MQ=255)
                                                                                                                                     cAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACGCt            <  1:4875647/150‑1 (MQ=255)
                                                                                                                                      aGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACGCTg           >  1:3470424/1‑150 (MQ=255)
                                                                                                                                      aGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACGCTg           <  1:4560341/150‑1 (MQ=255)
                                                                                                                                        aCTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCAAGTATATGCACATTACCAGCACCGAAGAAAAACGCTGGa         >  2:4928680/1‑150 (MQ=255)
                                                                                                                                          ttCCAGGAGACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACGCTGGATc       >  1:4617321/1‑150 (MQ=255)
                                                                                                                                            ccAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACGCTGGATCCa     <  1:586997/150‑1 (MQ=255)
                                                                                                                                             cAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACGCTGGATCCaa    <  1:149633/150‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
CAGGTTAAAGTCCTGCAGCTCATTAACGCATACCGCTTCCGTGGTCACCAGCATGCGAATCTCGATCCGCTGGGACTGTGGCAGCAAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGAGACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACGCTGGATCCAACA  >  CP009273/754426‑754718

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑