Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 772,664 A→C 16.1% K289N (AAA→AAC)  tolA → membrane anchored protein in TolA‑TolQ‑TolR complex

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273772,6640AC16.1% 376.3 / 32.2 179K289N (AAA→AAC) tolAmembrane anchored protein in TolA‑TolQ‑TolR complex
Reads supporting (aligned to +/- strand):  ref base A (105/41);  new base C (2/31);  total (107/72)
Fisher's exact test for biased strand distribution p-value = 1.18e-12
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 188 total aligned reads displayed.

CAGAGAAAGCTGCAGCCGACAAAAAAGCAGCAGAAAAAGCGGCTGCTGAAAAGGCAGCAGCTGATAAGAAAGCAGCGGCAGAAAAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACAATGGCGCATCAGGGGCCGATATCAATAA  >  CP009273/772516‑772813
                                                                                                                                                    |                                                                                                                                                     
  gagaAAGCTGCAGCCGACAAAAAAGCAGCAGAAAAAGCGGCTGCTGAAAAGGCAGCAGCTGATAAGAAAGCAGCGGCAGAAAAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCg                                                                                                                                                    <  2:4307031/150‑1 (MQ=255)
   agaAAGCTGCAGCCGACAAAAAAGCAGCAGAAAAAGCGGCTGCTGAAAAGGCAGCAGCTGATAAGAAAGCAGCGGCAGAAAAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAACGCgg                                                                                                                                                   >  2:495463/1‑150 (MQ=255)
    taatgatGCAGCATAAAAAAACCCAGAATAAAACGCGGTTGTTTAAAAGCACGCTGCTTATAACAAACCTGCGCAATAAAAAGCCGCCGCAGACAAAAAAGCGGCAGCGGAAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGc                                                                                                                                                  <  2:1316005/144‑1 (MQ=255)
     aaaGCTGCAGCCGACAAAAAAGCAGCAGAAAAAGCGGCTGCTGAAAAGGCAGCAGCTGATAAGAAAGCAGCGGCAGAAAAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGcc                                                                                                                                                 >  1:2644271/1‑150 (MQ=255)
     aaaGCTGCAGCCGACAAAAAAGCAGCAGAAAAAGCGGCTGCTGAAAAGGCAGCAGCTGATAAGAAAGCAGCGGCAGAAAAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGcc                                                                                                                                                 >  1:26020/1‑150 (MQ=255)
     aaaGCTGCAGCCGACAAAAAAGCAGCAGAAAAAGCGGCTGCTGAAAAGGCAGCAGCTGATAAGAAAGCAGCGGCAGAAAAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGcc                                                                                                                                                 >  2:3851439/1‑150 (MQ=255)
      aaGCTGCAGCCGACAAAAAAGCAGCAGAAAAAGCGGCTGCTGAAAAGGCAGCAGCTGATAAGAAAGCAGCGGCAGAAAAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCg                                                                                                                                                >  1:5164367/1‑150 (MQ=255)
       aGCTGCAGCCGACAAAAAAGCAGCAGAAAAAGCGGCTGCTGAAAAGGCAGCAGCTGATAAGAAAGCAGCGGCAGAAAAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAACAGCGGCCGc                                                                                                                                               >  1:5051752/1‑150 (MQ=255)
        gCTGCAGCCGACAAAAAAGCAGCAGAAAAAGCGGCTGCTGAAAAGGCAGCAGCTGATAAGAAAGCAGCGGCAGAAAAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCa                                                                                                                                              >  1:4253393/1‑150 (MQ=255)
           gCAGCCGACAAAAAAGCAGCAGAAAAAGCGGCTGCTGAAAAGGCAGCAGCTGATAAGAAAGCAGCGGCAGAAAAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCagag                                                                                                                                           >  2:2219991/1‑150 (MQ=255)
              gcctaaaaaaaaCCACCTGAAAATGCGGCTGCTTAAACGGATGCTGTTGATATGACTGCCGCGGATTAAAAAGCCGCCGCATACAAAAAAGCGGCAGCGTAAAAAGCTGCAGCTTAAAAAGCCGCTGATGCAAAAGCGGCCGCAGAGGCa                                                                                                                                        <  2:1239963/144‑1 (MQ=255)
               ccGACAAAACTGCAGCATAAACTGGGGCTGCTGAAATGGCAGCAGCTGATAAGAACGCAGCGGCACAAAAAGCCGCCGCAGAAAAAAAAGCGGCTGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAg                                                                                                                                       <  2:2575986/150‑1 (MQ=255)
                 gACAAAAAAGCAGCAGAAAAAGCGGCTGCTGAAAAGGCAGCAGCTGATAAGAAAGCAGCGGCAGAAAAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAgat                                                                                                                                     >  1:2486238/1‑150 (MQ=255)
                   cAAAAAAGCAGCAGAAAAAGCGGCTGCTGAAAAGGCAGCAGCTGATAAGAAAGCAGCGGCAGAAAAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCCGCAAAAGCGGCCGCAGAGGCAgatga                                                                                                                                   >  1:2165047/1‑150 (MQ=255)
                   cAAAAAAGCAGCAGAAAAAGCGGCTGCTGAAAAGGCAGCAGCTGATAAGAAAGCAGCGGCAGAAAAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAgatga                                                                                                                                   >  2:1874155/1‑150 (MQ=255)
                     aaaaaGCAGCAGAAAAAGCGGCTGCTGAAAAGGCAGCAGCTGATAAGAAAGCAGCGGCAGAAAAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGata                                                                                                                                 >  1:4466199/1‑150 (MQ=255)
                           cagcagAAAAAGCGGCTGCTGAAAAGGCAGCAGCTGATAAGAAAGCAGCGGCAGAAAAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCg                                                                                                                           >  1:3646608/1‑150 (MQ=255)
                               ataaaaaGCGGCTGCTTAAAAGGCTGCTGCTTATAAGACAGCTGCGGGATAAATCGCCGCCGCAGAAAAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGa                                                                                                                       <  2:4623276/148‑1 (MQ=255)
                                gAAAAAGCGGCTGCTGAAAAGGCAGCAGCTGATAAGAAAGCAGCGGCAGAAAAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCACAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAg                                                                                                                      >  1:1043761/1‑150 (MQ=255)
                                gAAAAAGCGGCTGCTGAAAAGGCAGCAGCTGATAAGAAAGCAGCGGCAGAAAAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGAGGATATTTTCGGTGAg                                                                                                                      >  1:815102/1‑150 (MQ=255)
                                gAAAAAGCGGCGGCTGAAACGGCAGCTGCTGATAAGAAAGCCGCGGCAGAAAAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAAAAGCTGCAGCTTAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAg                                                                                                                      <  2:3411858/150‑1 (MQ=255)
                                 aaaaaGCGGCTGCTGAAAAGGCAGCAGCTGATAAGAAAGCAGCGGCAGAAAAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCACAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGc                                                                                                                     >  2:1418303/1‑150 (MQ=255)
                                   aaaGCGGCTGCTGAAAAGGCAGCAGCTGATAAGAAAGCAGCGGCAGAAAAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTa                                                                                                                   >  1:3556248/1‑150 (MQ=255)
                                    acgCGGGTGCTTAAATGGATTCATCTTATAACAAAGCCGGGGAATAAAAAGCCGCCGCAGACAAAAACGCGGCAGCGGCAAAAGCTGCAGCTTAAAAAGCCGCTGCAGCAAACGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTaa                                                                                                                  <  2:4082759/148‑1 (MQ=255)
                                    aaGCGGCTGCTGAAAAGGCAGCAGCTGATAAGAAAGCAGCGGCAGAAAAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTac                                                                                                                  >  2:2718550/1‑149 (MQ=255)
                                    aaGCGGCTGCTGAAAAGGCAGCAGCTGATAAGAAAGCAGCGGCAGAAAAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTaa                                                                                                                  >  2:2940005/1‑150 (MQ=255)
                                    aaGCGGCTGCTGAAAAGGCAGCAGCTGATAAGAAAGCAGCGGCAGAAAAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTaa                                                                                                                  >  1:2284705/1‑150 (MQ=255)
                                    aaGCGGCTGCTGAAAAGGCAGCAGCTGATAAGAAAGCAGCGGCAGAAAAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTaa                                                                                                                  >  1:4867400/1‑150 (MQ=255)
                                     aGCGGCTGCTGAAAAGGCAGCAGCTGATAAGAAAGCAGCGGCAGAAAAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAg                                                                                                                 >  2:2445592/1‑150 (MQ=255)
                                       cGGCTGCTGAAAAGGCAGCAGCTGATAAGAAAGCAGCGGCAGAAAAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGct                                                                                                               >  1:1038109/1‑150 (MQ=255)
                                         gctgctTAAAAGGCAGCAGCTGATAAGAAAGCAGCGGCAGAAAAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAAACGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGctct                                                                                                             <  2:4267839/150‑1 (MQ=255)
                                          ttgctgAAACGCCAGCAGCTTATACCAACCCTGCGCCATAAACCGCCGCCCAACACAAAAACGCGGCTGCGGAAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAACGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTg                                                                                                            <  2:4812524/149‑1 (MQ=255)
                                          ctgctgACACGGCCGCAGCTGATACCCACGCCGCGGCAGAAAACGCCGCCGCCGACAAAAAAGCGGCAGCGGCAAACGCGGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTg                                                                                                            <  2:1652960/150‑1 (MQ=255)
                                           tgctgAAAAGGCAGCAGCTGATAAGAAAGCAGCGGCAGAAAAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTgg                                                                                                           >  2:805056/1‑150 (MQ=255)
                                           tgctgAAAAGGCAGCAGCTGATAAGAAAGCAGCGGCAGAAAAAGCCCCCGCAGACAAACAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTgg                                                                                                           <  2:3393941/150‑1 (MQ=255)
                                             cttaaacGGCTGCAGCTTATAATAACGCCGCGCCATAAACCGCCGCCGCAGACAAAAACGGGGCCGCGGCAACAGCTGCAGCTGAAAAAGCCGCTGCAGAAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTa                                                                                                         <  2:126340/147‑1 (MQ=255)
                                              tgAAAAGGCAGCAGCTGATAAGAAAGCAGCGGCAGAAAAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTaa                                                                                                        >  1:2943200/1‑150 (MQ=255)
                                               gAAAAGGCAGCAGCTGATAAGAAAGCAGCGGCAGAAAAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAg                                                                                                       >  2:4896287/1‑150 (MQ=255)
                                                 aaaGGCAGCAGCTGATAAGAAAGCAGCGGCAGAAAAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTACGaa                                                                                                     >  1:3915185/1‑150 (MQ=255)
                                                 aaaGGCAGCAGCTGATAAGAAAGCAGCGGCAGAAAAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGaa                                                                                                     >  1:3914967/1‑150 (MQ=255)
                                                        gctgttGATAATAACGCCGCGCCATAAACCGCCGCCGCAGACAAAAAAGCGGCTGCGGCAAAAGCTGCTGCTGAAAACGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCg                                                                                              <  2:4940024/145‑1 (MQ=255)
                                                             tGATAAGAAAGCAGCGGCAGAAAAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAAc                                                                                         >  1:221962/1‑150 (MQ=255)
                                                                 aaGAAAGCAGCGGCAGAAAAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACgggg                                                                                     >  1:4693783/1‑150 (MQ=255)
                                                                       gcagcGGCATAAAATGCCGCCGCATAAAAAAATGCGGCTGCGCAAAACGCTGATGCTTAAAAAGCCGCTGCAGAAAACGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAggg                                                                               <  2:1998091/150‑1 (MQ=255)
                                                                        cagcGGCATAAAATCCCGCCGCAGACACAAAAGCGGCTGCGGCAAAAGCTGCTGCTGAAAACGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAgggg                                                                              <  2:4661159/150‑1 (MQ=255)
                                                                         agcGGCAGAAAAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGc                                                                             >  2:2778651/1‑150 (MQ=255)
                                                                               aGAAAAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAgg                                                                       >  1:1267939/1‑150 (MQ=255)
                                                                                gAAAAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAggg                                                                      >  2:4169524/1‑150 (MQ=255)
                                                                                gAAAAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTATCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAggg                                                                      >  1:3192087/1‑150 (MQ=255)
                                                                                 aaaaaGCCGCCGCAGACAAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGa                                                                     >  2:4885443/1‑150 (MQ=255)
                                                                                  aaaaGCCGCCGCAGACAAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGaa                                                                    >  2:4490431/1‑150 (MQ=255)
                                                                                    caGCCGCCCAAGAAAAATATGCGCCTGCGCAAAAAGCTGCAGTTTAAAACGCCGCTGCAGAAAACGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACa                                                                  <  2:3089104/149‑1 (MQ=255)
                                                                                     aGCCGCCGCAGACAAAAAAGCGGCAGCGGCACAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACaa                                                                 >  1:4663686/1‑150 (MQ=255)
                                                                                          ccgcAGACAAAAAAGCGGCAGCGGAAAACGCGGCAGCTGAAAACCCCGCTGCAGCAAACGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCtt                                                            <  2:4566865/150‑1 (MQ=255)
                                                                                           cgcAGACAAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTc                                                           >  2:5029547/1‑150 (MQ=255)
                                                                                           cgcAGACAAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTc                                                           >  2:5048796/1‑150 (MQ=255)
                                                                                              aGACAACAAAGCGGCTGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGcc                                                        <  2:4298955/150‑1 (MQ=255)
                                                                                               taCAAAACAGCCGCAGCGGAAAACGCTCCGGCTTAAAACGCGGCGTCACAAAACGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGATTGCACCTAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCt                                                       <  1:1701111/149‑1 (MQ=255)
                                                                                               caaaaaaacGCGGCAGGGTAAAAAGCTGAAGCTTAAACACCCGCTGCAGAAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCt                                                       <  1:328472/147‑1 (MQ=255)
                                                                                                  aaaaatGCGGCAGGGGCAAACGCGGCAGCTTAAAACGCCGCTGCAGCAAAAGCGGCCGCAGTGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGcc                                                    <  2:2876281/150‑1 (MQ=255)
                                                                                                  aaaaaaGCGGCAGCGGAAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGcc                                                    <  2:2976229/150‑1 (MQ=255)
                                                                                                     aaaGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCACAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCggg                                                 >  2:3866896/1‑150 (MQ=255)
                                                                                                     aaaGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCggg                                                 >  1:2480811/1‑150 (MQ=255)
                                                                                                      cagcgcctgcggcAAAAGCTGCTGCTTAAAAAGCCGCGGCAGCAAACGCGGCCGCAGAGGCAGATGATCTTTTCGGTGAGCTAAGCTCTGGTAAGAAGGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGa                                                <  2:1715858/149‑1 (MQ=255)
                                                                                                      aaGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGa                                                >  1:161827/1‑150 (MQ=255)
                                                                                                      aaGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGa                                                >  1:3992837/1‑150 (MQ=255)
                                                                                                       agcggccgcggcAAACGCTGCAGCTTAAAACGCCGCTGCAGCAAACGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAg                                               <  2:265352/150‑1 (MQ=255)
                                                                                                         cggctgcgccaAAAGCTGCAGCTTAAAACGCCGCTGCACCAAACGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTg                                             <  2:3564047/150‑1 (MQ=255)
                                                                                                         cggccccggcaAACGCTGCTGCTAAAAACGCCGCGGCAGCAAACGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTg                                             <  1:4687862/144‑1 (MQ=255)
                                                                                                          ggcagcggcaAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTgg                                            >  2:5105967/1‑150 (MQ=255)
                                                                                                          ggcagcggcaAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTgg                                            >  2:4836416/1‑150 (MQ=255)
                                                                                                                  caAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTa                                    >  1:1846652/1‑150 (MQ=255)
                                                                                                                   aaaaGCTGCAGCTGAAAAAGCCTCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGTGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCAGGGAGGGGTAATACTaa                                   >  2:2340253/1‑150 (MQ=255)
                                                                                                                   aaaaGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAACACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTaa                                   >  1:1984849/1‑150 (MQ=255)
                                                                                                                    aaaGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTaaa                                  >  1:151647/1‑150 (MQ=255)
                                                                                                                          gCAGCTGAAAAAGCCGCGGCAGCAAACGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACAAt                            <  2:4727021/150‑1 (MQ=255)
                                                                                                                            aGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACAATgg                          >  1:3459026/1‑150 (MQ=255)
                                                                                                                             gcggAAACAGCCGCTGAAGAAAACGCGGCCGCTGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACAATGgc                         <  2:1303005/147‑1 (MQ=255)
                                                                                                                               ttaaaatGCCGCTGCAGAAAACGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACAATGgcgc                       <  2:161827/148‑1 (MQ=255)
                                                                                                                               tGAAAACGCCGGTGCAGAAAAAGCGGCCGCAGAGGCAGATGATAGTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACAATGgcgc                       <  2:4848463/150‑1 (MQ=255)
                                                                                                                               tGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACAATGgcgc                       >  1:1123418/1‑150 (MQ=255)
                                                                                                                                   aaaGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACAATGGCGCATCa                   >  1:997725/1‑150 (MQ=255)
                                                                                                                                    acgCCGGTGCACAAAACGCGGCCGCAGAGGCAGATGATATTTTCGGTGTGCTAAGCTCTGGTATGAATGCACCTAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACAATGGCGCATCAg                  <  1:3360983/148‑1 (MQ=255)
                                                                                                                                     cgCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACAATGGCGCATCAgg                 <  2:3285881/149‑1 (MQ=255)
                                                                                                                                     ccccGCTGCACAAAACGCGGCCGCTGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCTAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACAATGGCGCATCAgg                 <  1:233028/148‑1 (MQ=255)
                                                                                                                                       ccGCTGCACAAAACGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGATAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACAATGGCGCATCAgggg               <  2:2308104/150‑1 (MQ=255)
                                                                                                                                       ccGCTGAAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACAATGGCGCATCAgggg               <  2:723320/150‑1 (MQ=255)
                                                                                                                                         gCTGCAGAAACCGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGATTGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACAATGGCGCATCAGGGGcc             <  2:112382/150‑1 (MQ=255)
                                                                                                                                          cTGCATAAAAAGCGGCCGCTGTGGATGTTGATATTTTGGGTGAGCTTTGTTTTGGTAATAATGCACCTAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACAATGGCGCATCAGGGGCCg            <  1:4454736/150‑1 (MQ=255)
                                                                                                                                          cTGCAGCAAACGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACAATGGCGCATCAGGGGCCg            >  1:4913439/1‑150 (MQ=255)
                                                                                                                                          cTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACAATGGCGCATCAGGGGCCg            >  2:1310925/1‑150 (MQ=255)
                                                                                                                                            gcagcaAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACAATGGCGCATCAGGGGCCGat          <  2:2486238/150‑1 (MQ=255)
                                                                                                                                              agcaAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACACTGGCGCATCAGGGGCCGatct        >  2:2527061/1‑148 (MQ=255)
                                                                                                                                              agaaaaaGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACAATGGCGCATCAGGGGCCGatat        <  2:2088862/147‑1 (MQ=255)
                                                                                                                                                aaaacGCGGCCGCAGCGGCATATGTTATTTTCGGTGAGCTACGCTCTGGTATGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACAATGGCGCATCAGGGGCCGATATCa      <  1:2049862/149‑1 (MQ=255)
                                                                                                                                                 aaacGCGGCCGCAGAGGCAGATTATATTTTCGGTGAGCTAAGCTCTGTTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACAATGGCGCATCAGGGGCCGATATCaa     <  1:777753/150‑1 (MQ=255)
                                                                                                                                                 aaaaGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTCAAAACAATGGCGCATCAGGGGCCGATATCaa     >  2:1508554/1‑150 (MQ=255)
                                                                                                                                                 aaaaGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACAATGGCGCATCAGGGGCCGATATCaa     >  2:1508532/1‑150 (MQ=255)
                                                                                                                                                   aaGCGGCCGCAGCGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTACGACTGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACAATGGCGCATCAGGGGCCGATATCAATa   <  2:2454795/150‑1 (MQ=255)
                                                                                                                                                    aGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACAATGGCGCATCAGGGGCCGATATCAATaa  >  2:3997952/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
CAGAGAAAGCTGCAGCCGACAAAAAAGCAGCAGAAAAAGCGGCTGCTGAAAAGGCAGCAGCTGATAAGAAAGCAGCGGCAGAAAAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACAATGGCGCATCAGGGGCCGATATCAATAA  >  CP009273/772516‑772813

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑