Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 800,779 G→T 94.9% E607D (GAG→GAT)  ybhJ → putative hydratase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273800,7790GT94.9% 707.1 / 24.0 217E607D (GAG→GAT) ybhJputative hydratase
Reads supporting (aligned to +/- strand):  ref base G (8/3);  new base T (99/107);  total (107/110)
Fisher's exact test for biased strand distribution p-value = 1.31e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.35e-01

Only 100 of 225 total aligned reads displayed.

CGGTGAAACCTCTTCTTATCGTTCAAATCCGATTGGTCTGGCGGAGTTTACCCTGTCTCGCCGCGATCCCGGTTATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGAGGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGCGATGGTTCTGCGCGTGAACAGGCGGCGAGCTGCCAGCGTGTGATTGGCGGTCTGG  >  CP009273/800632‑800927
                                                                                                                                                   |                                                                                                                                                    
cGGTGAAACCTCTTCTTATCGTTCAAATCCGATTGGTCTGGCGGAGTTTACCCTGTCTCGCCGCGATCCCGGTTATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATgt                                                                                                                                                    <  2:1065744/150‑1 (MQ=255)
cGGTGAAACCTCTTCTTATCGTTCAAATCCGATTGGTCTGGCGGAGTTTACCCTGTCTCGCCGCGATCCCGGTTATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATgt                                                                                                                                                    >  2:1107959/1‑150 (MQ=255)
cGGTGAAACCTCTTCTTATCGTTCAAATCCGATTGGTCTGGCGGAGTTTACCCTGTCTCGCCGCGATCCCGGTGATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATgt                                                                                                                                                    >  2:1108136/1‑150 (MQ=255)
 ggTGAAACCTCTTCTTATCGTTCAAATCCGATTGGTCTGGCGGAGTTTACCCTGTCTCGCCGCGATCCCGGTTATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGAtgtg                                                                                                                                                   >  2:3299580/1‑150 (MQ=255)
 ggTGAAACCTCTTCTTATCGTTCAAATCCGATTGGTCTGGCGGAGTTTACCCTGTCTCGCCGCGATCCCGGTTATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGAtgtg                                                                                                                                                   >  1:2127313/1‑150 (MQ=255)
    taaaCCTGTTCTTATCGTTCAAATCCGATTGGTCTGGCGGATTTTACCCTGTCTCGCCGCGATCCCGGTTATGTTAGCAGAAGTAACGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGttt                                                                                                                                                <  1:3996356/149‑1 (MQ=255)
    gAAACCTCTTCTTATCGTTCAAATCCGATTGGTCTGGCGGAGTTTACCCTGTCTCGCCGCGATCCCGGTTATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGttt                                                                                                                                                <  2:804631/150‑1 (MQ=255)
    caaaCTTCTTTTTTTCTTAAAATTCCGTTTGGTGGGGCGTTTTTTACCCTGTTTCGCCGCGTTCCCGTTTTTTTTACCATATTTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGttt                                                                                                                                                <  1:612149/149‑1 (MQ=255)
     aaaCCTCTTCTTATCGTTCAAATCCGATTGGTCTGGCGGAGTTTACCCTGTCTCGCCGCGATCCCGGTTATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTg                                                                                                                                               <  2:4486739/150‑1 (MQ=255)
       aCCTCTTCTTATCGTTCAAATCCGATTGGTCTGGCGGAGTTTACCCTGTCTCGCCGCGATCCCGGTTATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTgcg                                                                                                                                             >  1:538014/1‑150 (MQ=255)
           cttcttATCGTTCAAATCCGATTGGTCTGGCGGAGTTTACCCTGTCTCGCCGCGATCCCGGTTATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCa                                                                                                                                         >  2:1816193/1‑150 (MQ=255)
           cttcttATCGTACAAATCCGATTGGTGTGGCGGAGTTTACCCTGTCCCGCCGCGATCCCGGTTTGTTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCa                                                                                                                                         <  1:3498482/150‑1 (MQ=255)
             tcttATCGTTCAAATCCGATTGGTCTGGCGGAGTTTACCCTGTCTCGCCGCGATCCCGGTTATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCAtt                                                                                                                                       >  1:5168192/1‑150 (MQ=255)
              cttATCGTTCAAATCCGTTTGGTCTGGCGGTGTTTACCCTGTCTCGCCGCGATCCCGGTTATGTTAGCAGAATTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTa                                                                                                                                      <  1:3098823/150‑1 (MQ=255)
              cttATCGTTCAAATCCGATTGGTCTGGCGGAGTTTACCCTGTCTCGCCGCGATCCCGGTTATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTa                                                                                                                                      >  2:2776223/1‑150 (MQ=255)
              cttATCGTTCAAATCCGATTGGTCTGGCGGAGTTTACCCTGTCTCGCCGCGATCCCGGTTATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTa                                                                                                                                      >  1:1820199/1‑150 (MQ=255)
                tATCGTTCAAATCCGTTTGGTCTGGCGGAGTTTACCCTGTCTCGCCGCGATCCCGGTTATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAg                                                                                                                                    <  2:3695713/150‑1 (MQ=255)
                  tCGTTCAAATCCGTTTGGTCTGGGGGAGTTTACCCTGTCTCGCCGCGATCCCGTTTATGTTAGCAGAAGAAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCa                                                                                                                                  <  1:3763235/150‑1 (MQ=255)
                  tCGTTCAAATCCGATTGGTCTGGCGGAGTTTACCCTGTCTCGCCGCGATCCCGGTTATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCa                                                                                                                                  >  1:5062917/1‑150 (MQ=255)
                   cGTTCAAATCCGATTGGTCTGGCGGAGTTTACCCTGTCTCGCCGCGATCCCGGTTATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAg                                                                                                                                 >  2:725938/1‑150 (MQ=255)
                   cGTTCAAATCCGATTGGTCTGGCGGAGTTTACCCTGTCTCGCCGCGATCCCGGTTATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAg                                                                                                                                 <  2:1945078/150‑1 (MQ=255)
                   cGTTCAAATCCGATTGGTCTGGCGGAGTTTACCCTGTCTCGCCGCGATCCCGGTTATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAg                                                                                                                                 >  2:2053928/1‑150 (MQ=255)
                   cGTTCAAATCCGATTGGTCTGGCGGAGTTTACCCTGTCTCGCCGCGATCCCGGTTATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGAGGTGTTTGCGCGCATTAAGCAg                                                                                                                                 >  1:1995605/1‑150 (MQ=255)
                        aaaTCCGTTTGGTCTGGCGGAGTTTACCCTGTCTCGCCGCGATCCCGGTTTTGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGc                                                                                                                            <  1:3898250/150‑1 (MQ=255)
                        aaaTCCGATTGGTCTGGCGGATTTTACCCTGTCTCGCCGCTATCCCGGTTATGTTAGAAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGc                                                                                                                            <  1:1301571/150‑1 (MQ=255)
                        aaaTCCGATTGGTCTGGCGGAGTTTACCCTGTCTCGCCGCGATCCCGGTTATGTTAGCAGAAGTAACGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGc                                                                                                                            <  2:1737476/150‑1 (MQ=255)
                         aaTCCGATTGGTCTGGCGGAGTTTACCCTGTCTCGCCGCGATCCCGGTTATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCt                                                                                                                           <  2:801719/150‑1 (MQ=255)
                         aaTCCGATTGGTCTGGCGGAGTTTACCCTGTCTCGCCGCGATCCCGGTTATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCt                                                                                                                           <  2:381320/150‑1 (MQ=255)
                              gATTGGTCTGGCGGAGTTTACCCTGTCTCGCCGCGATCCCGGTTATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCa                                                                                                                      <  2:243215/150‑1 (MQ=255)
                               aTTGGTCTGGCGGAGTTTACCCTGTCTCGCCGCGATCCCGGTTATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAg                                                                                                                     <  2:2976212/150‑1 (MQ=255)
                                ttGGTCTGGCGTATTTTACCCTGTCTCGCCGCTATCCCGTTTATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAgg                                                                                                                    <  1:5016128/150‑1 (MQ=255)
                                ttGGTCGGGCGTTGTTTACCCTGTCTCGCCGCGTTCCCGGTTTTGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAgg                                                                                                                    <  1:2001512/150‑1 (MQ=255)
                                  ggTCTGGCGGTGTTAACCGTGTCTCGCCGCGATCCGGGTTTTGTTACCATAATTAACGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAg                                                                                                                  <  1:2231569/150‑1 (MQ=255)
                                   gTCTGGCGGTGTTTACCCTGTCTCGCCGCGATCCCGGTTTTGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGc                                                                                                                 <  1:842735/150‑1 (MQ=255)
                                        gCGGAGTTTACCCTGTCTCGCCGCGATCCCGGTTATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATAtt                                                                                                            <  2:5084890/150‑1 (MQ=255)
                                         cGGAGTTTACCCTGTCTCGCCGCGATCCCGGTTATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTg                                                                                                           >  2:1778945/1‑150 (MQ=255)
                                         cGGAGTGTACCCGGTCTCGCCGCGATCCCGTTTATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTg                                                                                                           <  2:3171145/150‑1 (MQ=255)
                                           gAGTTTACCCTGTCTCGCCGCGATCCCGGTTATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGAt                                                                                                         >  1:2526557/1‑150 (MQ=255)
                                             gTTTACCCTGTCTCGCCGCGATCCCGGTTATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATcc                                                                                                       >  1:4049185/1‑150 (MQ=255)
                                             gTTTACCCTGTCTCGCCGCGATCCCGGTTATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATcc                                                                                                       >  2:2937262/1‑150 (MQ=255)
                                              tttACCCTGTCTCGCCGCGATCCCGGTTATGTTAGCAGATGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCg                                                                                                      <  1:821160/150‑1 (MQ=255)
                                               ttACCCTGTCTCGCCGCGATCCCGGTTATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGc                                                                                                     <  1:5176303/150‑1 (MQ=255)
                                                tACCCTGTCTCGCCGCGATCCCGGTTATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCt                                                                                                    >  2:3025664/1‑150 (MQ=255)
                                                     tGTCTCGCCGCGATCCCGGTTATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCaaa                                                                                               >  2:19719/1‑150 (MQ=255)
                                                       tctcGCCGCGATCCCGGTTATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACt                                                                                             <  2:1890754/150‑1 (MQ=255)
                                                         tcGCCGCGATCCCGGTTATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGa                                                                                           >  1:45/1‑150 (MQ=255)
                                                          cgccgcGATCCCGTTTATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGaa                                                                                          <  2:617687/150‑1 (MQ=255)
                                                          cgccgcGATCCCGGTTATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGaa                                                                                          >  1:942268/1‑150 (MQ=255)
                                                                  tCCGGGTTTTGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGgcag                                                                                  <  1:2626916/150‑1 (MQ=255)
                                                                  tCCCGGTTATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGgcag                                                                                  >  1:1132361/1‑150 (MQ=255)
                                                                      ggTTATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGAGGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATg                                                                              >  2:661302/1‑150 (MQ=255)
                                                                          tttttAGCACAATTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCt                                                                          <  1:2201344/147‑1 (MQ=255)
                                                                           tGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTa                                                                         <  2:121399/150‑1 (MQ=255)
                                                                           tGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCAGTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTa                                                                         <  2:2632079/150‑1 (MQ=255)
                                                                             ttAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATg                                                                       >  1:638216/1‑150 (MQ=255)
                                                                             ttAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATg                                                                       >  1:3244239/1‑150 (MQ=255)
                                                                              tAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGc                                                                      <  2:436465/150‑1 (MQ=255)
                                                                               aGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCg                                                                     <  2:1136603/150‑1 (MQ=255)
                                                                                 cctacttAACGCGACGGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGt                                                                   <  1:336054/144‑1 (MQ=255)
                                                                                 cAGAAGTATAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGt                                                                   <  1:4708780/150‑1 (MQ=255)
                                                                                  aGAAGTAACGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTg                                                                  <  1:3677385/150‑1 (MQ=255)
                                                                                  aGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTg                                                                  >  2:4607265/1‑150 (MQ=255)
                                                                                   tatttAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGa                                                                 <  1:2413016/146‑1 (MQ=255)
                                                                                    aaGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGaa                                                                >  2:82805/1‑150 (MQ=255)
                                                                                    aaGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGaa                                                                >  2:4933465/1‑150 (MQ=255)
                                                                                     aGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGACATTGGCAGCATGGTCTATGCGGTGaaa                                                               >  2:3913221/1‑150 (MQ=255)
                                                                                        aaaGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCa                                                            <  1:1914590/150‑1 (MQ=255)
                                                                                             gACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGCGa                                                       >  1:3603234/1‑150 (MQ=255)
                                                                                               ctgctgAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGCGATg                                                     <  1:1101710/150‑1 (MQ=255)
                                                                                               ctgctgAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGCGATg                                                     <  1:3845655/150‑1 (MQ=255)
                                                                                                   tgAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGCGATGGTTc                                                 >  2:4581146/1‑150 (MQ=255)
                                                                                                    gAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGCGATGGTTCt                                                >  2:294470/1‑150 (MQ=255)
                                                                                                    gAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGCGATGGTTCt                                                >  2:424841/1‑150 (MQ=255)
                                                                                                    gAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGCGATGGTTCt                                                >  2:3829492/1‑150 (MQ=255)
                                                                                                      gCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGCGATGGTTCTgc                                              <  1:683473/150‑1 (MQ=255)
                                                                                                       cTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGCGATGGTTCTgcg                                             >  1:1045936/1‑150 (MQ=255)
                                                                                                          gAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGCGATGGTTCTGCGCGt                                          <  2:1516883/150‑1 (MQ=255)
                                                                                                             aaTCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGCGATGGTTCTGCGCGTGaa                                       >  1:1569719/1‑150 (MQ=255)
                                                                                                                     tCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGCGATGGTTCTGCGCGTGAACAggcggc                               <  1:4140704/150‑1 (MQ=255)
                                                                                                                       tGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGCGATGGTTCTGCGCGTGAACAGGCGGCGa                             >  2:230298/1‑150 (MQ=255)
                                                                                                                       tGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGCGATGGTTCTGCGCGTGAACAGGCGGCGa                             >  1:3429036/1‑150 (MQ=255)
                                                                                                                       tGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGCGATGGTTCTGCGCGTGAACAGGCGGCGa                             >  1:5176402/1‑150 (MQ=255)
                                                                                                                          cGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGCGATGGTTCTGCGCGTGAACAGGCGGCGAGCt                          >  2:2073005/1‑150 (MQ=255)
                                                                                                                           ggggAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGCGATGGTTCTGCGCGTGAACAGGCGGCGAGCTg                         >  1:742209/1‑150 (MQ=255)
                                                                                                                            gggAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGCGATGGTTCTGCGCGTGAACAGGCGGCGAGCTGc                        <  1:514708/150‑1 (MQ=255)
                                                                                                                               aaTGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGCGATGGTTCTGCGCGTGAACAGGCGGCGAGCTGCCAg                     >  1:4371187/1‑150 (MQ=255)
                                                                                                                               aaTGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGCGATGGTTCTGCGCGTGAACAGGCGGCGAGCTGCCAg                     >  1:283563/1‑150 (MQ=255)
                                                                                                                               aaTGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGCGATGGTTCTGCGCGTGAACAGGCGGCGAGCTGCCAg                     >  1:1855315/1‑150 (MQ=255)
                                                                                                                                   tCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGCGATGGTTCTGCGCGTGAACAGGCGGCGAGCTGCCAGCgtg                 <  1:1107962/150‑1 (MQ=255)
                                                                                                                                   tCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGCGATGGTTCTGCGCGTGAACAGGCGGCGAGCTGCCAGCgtg                 <  1:1964004/150‑1 (MQ=255)
                                                                                                                                        gAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGCGATGGTTCTGCGCGTGAACAGGCGGCGAGCTGCCAGCGTGTGAtt            <  2:2356177/150‑1 (MQ=255)
                                                                                                                                         aGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGCGATGGTTCTGCGCGTGAACAGGCGGCGAGCTGCCAGCGTGTGATTg           <  2:3241462/150‑1 (MQ=255)
                                                                                                                                         aGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGCGATGGTTCTGCGCGTGAACAGGCGGCGAGCTGCCAGCGTGTGATTg           >  1:4362704/1‑150 (MQ=255)
                                                                                                                                           cTGACAGAGGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGCGATGGTTCTGCGCGTGAACAGGCGGCGAGCTGCCAGCGTGTGATTGGc         >  2:1326782/1‑150 (MQ=255)
                                                                                                                                            tGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGCGATGGTTCTGCGCGTGAACAGGCGGCGAGCTGCCAGCGTGTGATTGGCg        >  2:911059/1‑150 (MQ=255)
                                                                                                                                              aCAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGCGATGGTTCTGCGCGTGAACAGGCGGCGAGCTGCCAGCGTGTGATTGGCGGt      >  1:4420497/1‑150 (MQ=255)
                                                                                                                                              aCAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGCGATGGTTCTGCGCGTGAACAGGCGGCGAGCTGCCAGCGTGTGATTGGCGGt      <  2:1377080/150‑1 (MQ=255)
                                                                                                                                               cAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGCGATGGTTCTGCGCGTGAACAGGCGGCGAGCTGCCAGCGTGTGATTGGCGGTc     <  2:4548354/150‑1 (MQ=255)
                                                                                                                                               cAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGCGATGGTTCTGCGCGTGAACAGGCGGCGAGCTGCCAGCGTGTGATTGGCGGTc     <  2:4123637/150‑1 (MQ=255)
                                                                                                                                                agatGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGCGATGGTTCTGCGCGTGAACAGGCGGCGAGCTGCCAGCGTGTGATTGGCGGTCt    <  1:2226891/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
CGGTGAAACCTCTTCTTATCGTTCAAATCCGATTGGTCTGGCGGAGTTTACCCTGTCTCGCCGCGATCCCGGTTATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGAGGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGCGATGGTTCTGCGCGTGAACAGGCGGCGAGCTGCCAGCGTGTGATTGGCGGTCTGG  >  CP009273/800632‑800927

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑