Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 832,445 G→T 37.2% P213P (CCG→CCT)  ybiC → putative dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273832,4450GT37.2% 303.1 / 261.1 231P213P (CCG→CCT) ybiCputative dehydrogenase
Reads supporting (aligned to +/- strand):  ref base G (77/68);  new base T (53/33);  total (130/101)
Fisher's exact test for biased strand distribution p-value = 2.20e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 240 total aligned reads displayed.

CCGTTCTGTGTGGTTTTCCCTCGTAAAGATAATTTCCCGCTGTTGCTTGATTACGCCACCAGCGCCATTGCATTTGGCAAAACCCGCGTCGCCTGGCATAAAGGCGTCCCCGTGCCGCCAGGTTGCCTGATTGACGTTAACGGCGTGCCGACGACCAATCCGGCGGTAATGCAGGAGTCGCCGTTGGGTTCGCTGTTGACCTTTGCCGAACATAAAGGCTACGCCCTTGCAGCGATGTGTGAAATTCTTGGCGGGGCGCTTTCCGGCGGTAAAACGACGCATCAGGAAACGTTACAAAC  >  CP009273/832296‑832594
                                                                                                                                                     |                                                                                                                                                     
ccGTTCTGTGTGGTTTTCCCTCGTAAAGATAATTTCCCGCTGTTGCTTGATTACGCCACCCGCGCCATTGCATTTGGCAAAACCCGCGTCGCCTGGCATAAAGGCGTCCCCGTGCCGCCAGGTTGCCTGATTGACGTTAACGGCGTGCcg                                                                                                                                                       >  1:1317829/1‑150 (MQ=255)
   ttCTGTGTGGTTTTCCCTCGTAAAGATAATTTCCCGCTGTTGCTTGATTACGCCACCAGCGCCATTGCATTTGGCAAAACCCGCGTCGCCTGGCATAAAGGCGTCCCCGTGCCGCCAGGTTGCCTGATTGACGTTAACGGCGTGCcgacg                                                                                                                                                    <  2:2154271/150‑1 (MQ=255)
     cTGTGTGGTTTTCCCTCGTAAAGATAATTTCCCGCTGTTGCTTGATTACGCCACCAGCGCCATTGCATTTGGCAAAACCCGCGTCGCCTGGCATAAAGGCGTCCCCGTGCCGCCAGGTTGCCTGATTGACGTTAACGGCGTGCCgacgac                                                                                                                                                  >  1:3008230/1‑150 (MQ=255)
      tgtgtgGTTTTCCCTCGTAAAGATAATTTCCCGCTGTTGCTTGATTACGCCACCAGCGCCATTGCATTTGGCAAAACCCGCGTCGCCTGGCATAAAGGCGTCCCCGTGCCGCCAGGTTGCCTGATTGACGTTAACGGCGTGCCTACGAcc                                                                                                                                                 >  1:4248799/1‑150 (MQ=255)
       gtgtgGTTTTCCCTCGTAAAGATACTTTCCCGCTGTTGCTTGATTACGCCACCAGCGCCATTGCATTTGGCAAAACCCGCGTCGCCTGGCATAAAGGCGTCCCCGTGCCGCCAGGTTGCCTGATTGACGTTAACGGCGTGCCGACGACCa                                                                                                                                                <  1:3241476/150‑1 (MQ=255)
       gtgtgGTTTTCCCTCGTAAAGATAATTTCCCGCTGTTGCTTGATTACGCCACCAGCGCCATTGCATTTGGCAAAACCCGCGTCGCCTGGCATAAAGGCGTCCCCGTGCCGCCAGGTTGCCTGATTGACGTTAACGGCGTGCCGACGACCa                                                                                                                                                <  2:756070/150‑1 (MQ=255)
         gtgGTTTTCCCTCGTAAAGATAATTTCCCGCTGTTGCTTGATTACGCCACCAGCGCCATTGCATTTGGCAAAACCCGCGTCGCCTGGCATAAAGGCGTCCCCGTGCCGCCAGGTTGCCTGATTGACGTTAACGGCGTGCCGACGACCAAt                                                                                                                                              <  1:4518404/150‑1 (MQ=255)
          tgGTTTTCCCTCGTAAAGATAATTTCCCGCTGTTGCTTGATTACGCCACCAGCGCCATTGCATTTGGCAAAACCCGCGTCGCCTGGCATAAAGGCGTCCCCGTGCCGCCAGGTTGCCTGATTGACGTTAACGGCGTGCCGACGACCAATc                                                                                                                                             >  2:501574/1‑150 (MQ=255)
          tgGTTTTCCCTCGTAAAGATAATTTCCCGCTGGTGCTTGATTACGCCACCAGCGCCATTGCATTTGGCAAAACCCGCGTCGCCTGGCATAAAGGCGTCCCCGTGCCGCCAGGTTGCCTGATTGACGTTAACGGCGTGCCGACGACCAATc                                                                                                                                             <  1:442505/150‑1 (MQ=255)
           ggTTTTCCCTCGTAAAGATAATTTCCCGCTGGTGCTTGATTACGCCACCAGCGCCATTGCATTTGGCAAAACCCGCGTCGCCTGGCATAAAGGCGTCCCCGTGCCGCCAGGTTGCCTGATTGACGTTAACGGCGTGCCGACGACCAATcc                                                                                                                                            <  2:1813641/150‑1 (MQ=255)
           ggTTTTCCCTCGTAAAGATAATGTCCCGCTGTTGCTTGATTACGCCACCAGCGCCATTGCATTTGGCAAAACCCGCGTCGCCTGGCATAAAGGCGTCCCCGTGCCGCCAGGTTGCCTGATTGACGTTAACGGCGTGCCTACGACCAATcc                                                                                                                                            <  2:5167569/150‑1 (MQ=255)
                  ccTCGTAAAGATAATTTCCCGCTGTTGCTTGATTACGCCACCAGCGCCATTGCATTTGGCAAAACCCGCGTCGCCTGGCATAAAGGCGTCCCCGTGCCGCCAGGTTGCCTGATTGACGTTAACGGCGTGCCGACGACCAATCCGGCGGTa                                                                                                                                     >  2:2015760/1‑150 (MQ=255)
                    tCGTAAAGATAATTTCCCGCTGTTGCTTGATTACGCCACCAGCGCCATTGCATTTGGCAAAACCCGCGTCGCCTGGCATAAAGGCGTCCCCGTGCCGCCAGGTTGCCTGATTGACGTTAACGGCGTGCCGACGACCAATCCGGCGGTAAt                                                                                                                                   <  1:3190234/150‑1 (MQ=255)
                     cGTAAAGATAATTTCCCGCTGTTGCTTGATTACGCCACCAGCGCCATTGCATTTGGCAAAACCCGCGTCGCCTGGCATAAAGGCGTCCCCGTGCCGCCAGGTTGCCTGATTGACGTTAACGGCGTGCCTACGACCAATCCGGCGGTAATg                                                                                                                                  <  2:277919/150‑1 (MQ=255)
                      gTAAAGATAATTTCCCGCTGTTGCTTGATTACGCCACCAGCGCCATTGCATTTGGCAAAACCCGCGTCGCCTGGCATAAAGGCGTCCCCGTGCCGCCAGGTTGCCTGATTGACGTTAACGGCGTGCCGACGACCAATCCGGCGGTAATGc                                                                                                                                 >  2:3467640/1‑150 (MQ=255)
                       tAAAGATAATGTCCCGCTGTTGCTTGATTACGCCACCAGCGCCATTGCATTTGGCAAAACCCGCGTCGCCTGGCATAAAGGCGTCCCCGTGCCGCCAGGTTGCCTGATTGACGTTAACGGCGTGCCTACGACCAATCCGGCGGTAATGCa                                                                                                                                <  2:3008043/150‑1 (MQ=255)
                        aaaGATAATTTCCCGCTGTTGCTTGATTACGCCACCAGCGCCATTGCATTTGGCAAAACCCGCGTCGCCTGGCATAAAGGCGTCCCCGTGCCGCCAGGTTGCCTGATTGACGTTAACGGCGTGCCGACGACCAATCCGGCGGTAATGCAg                                                                                                                               >  2:358567/1‑150 (MQ=255)
                        aaaGATAATGTCCCGCTGTTGCTTGATTACGCCACCAGCGCCATTGCATTTGGCAAAACCCGCGTCGCCTGGCATAAAGGCGTCCCCGTGCCGCCAGGTTGCCTGATTGACGTTAACGGCGTGCCGACGACCAATCCGGCGGTAATGCAg                                                                                                                               <  1:3132346/150‑1 (MQ=255)
                         aaGATAATTTCCCGCTGTTGCTTGATTACGCCACCAGCGCCATTGCATTTGGCAAAACCCGCGTCGCCTGGCATAAAGGCGTCCCCGTGCCGCCAGGTTGCCTGATTGACGTTAACGGCGTGCCTACGACCAATCCGGCGGTAATGCAgg                                                                                                                              <  2:3786497/150‑1 (MQ=255)
                         aaGATAATTTCCCGCTGTTGCTTGATTACGCCACCAGCGCCATTGCATTTGGCAAAACCCGCGTCGCCTGGCATAAAGGCGTCCCCGTGCCGCCAGGTTGCCTGATTGACGTTAACGGCGTGCCTACGACCAATCCGGCGGTAATGCAgg                                                                                                                              >  1:3744197/1‑150 (MQ=255)
                           gATAATTTCCCGCTGTTGCTTGATTACGCCACCAGCGCCATTGCATTTGGCAAAACCCGCGTCGCCTGGCATAAAGGCGTCCCCGTGCCGCCAGGTTGCCTGATTGACGTTAACGGCGTGCCGACGACCAATCCGGCGGTAATGCAGGAg                                                                                                                            <  1:704835/150‑1 (MQ=255)
                            aTAATTTCCCGCTGTTGCTTGATTACGCCACCAGCGCCATTGCATTTGGCAAAACCCGCGTCGCCTGGCATAAAGGCGTCCCCGTGCCGCCAGGTTGCCTGATTGACGTTAACGGCGTGCCTACGACCAATCCGGCGGTAATGCAGGAGt                                                                                                                           >  1:4931730/1‑150 (MQ=255)
                                tttCCCGCTGTTGCTTGATTACGCCACCAGCGCCATTGCATTTGGCAAAACCCGCGTCGCCTGGCATAAAGGCGTCCCCGTGCCGCCAGGTTGCCTGATTGACGTTAACGGCGTGCCTACGACCAATCCGGCGGTAATGCAGGAGTCGcc                                                                                                                       <  1:5034237/150‑1 (MQ=255)
                                tttCCCGCTGTTGCTTGATTACGCCACCAGCGCCATTGCATTTGGCAAAACCCGCGTCGCCTGGCATAAAGGCGTCCCCGTGCCGCCAGGTTGCCTGATTGACGTTAACGGCGTGCCTACGACCAATCCGGCGGTAATGCAGGAGTCGcc                                                                                                                       <  1:5035017/150‑1 (MQ=255)
                                 ttCCCGCTGTTGCTTGATTACGCCACCAGCGCCATTGCATTTGGCAAAACCCGCGTCGCCTGGCATAAAGGCGTCCCCGTGCCGCCAGGTTGCCTGATTGACGTTAACGGCGTGCCGACGACCAATCCGGCGGTAATGCAGGAGTCGCCg                                                                                                                      <  2:375725/150‑1 (MQ=255)
                                     cGCTGTTGCTTGATTACGCCACCAGCGCCATTGCATTTGGCAAAACCCGCGTCGCCTGGCATAAAGGCGTCCCCGTGCCGCCAGGTTGCCTGATTGACGTTAACGGCGTGCCTACGACCAATCCGGCGGTAATGCAGGAGTCGCCGTTgg                                                                                                                  <  1:22867/150‑1 (MQ=255)
                                       cTGTTGCTTGATTACGCCACCAGCGCCATTGCATTTGGCAAAACCCGCGTCGCCTGGCATAAAGGCGTCCCCGTGCCGCCAGGTTGCCTGATTGACGTTAACGGCGTGCCGACGACCAATCCGGCGGTAATGCAGGAGTCGCCGTTGGGt                                                                                                                >  1:2225159/1‑150 (MQ=255)
                                         gTTGCTTGATTACGCCACCAGCGCCATTGCATTTGGCAAAACCCGCGTCGCCTGGCATAAAGTCGTCCCCGTGCCGCCAGGTTGCCTGAATGACGATAACCGCGTGCCTACGACCAATCCGGCGGTAATGCAGGAGTCGCCGTAGGGTTc                                                                                                              >  1:3405945/1‑150 (MQ=255)
                                         gTTGCTTGATTACGCCACCAGCGCCATTGCATTTGGCAAAACCCGCGTCGCCTGGCATAAAGGCGTCCCCGTGCCGCCAGGTTGCCTGATTGACGTTAACGGCGTGCCTACGACCAATCCGGCGGTAATGCAGGAGTCGCCGTTGGGTTc                                                                                                              >  1:3199791/1‑150 (MQ=255)
                                         gTTGCTTGATTACGCCACCAGCGCCATTGCATTTGGCAAAACCCGCGTCGCCTGGCATAAAGGCGTCCCCGTGCCGCCAGGTTGCCTGATTGACGTTAACGGCGTGCCGACGACCAATCCGGCGGTAATGCAGGAGTCGCCGTTGGGTTc                                                                                                              >  2:618326/1‑150 (MQ=255)
                                              ttGATTACGCCACCAGCGCCATTGCATTTGGCAAAACCCGCGTCGCCTGGCATAAAGGCGTCCCCGTGCCGCCAGGTTGCCTGATTGACGTTAACGGCGTGCCGACGACCAATCCGGCGGTAATGCAGGAGTCGCCGTTGGGTTCGCTGt                                                                                                         >  2:1879366/1‑150 (MQ=255)
                                              ttGATTACGCCACCAGCGCCATTGCATTTGACAAAACCCGCGTCGCCTGGCATAAAGGCGTCCCCGTGCCGCCAGGTTGCCTGATTGACGTTAACGGCGTGCCGACGACCAATCCGGCGGTAATGCAGGAGTCGCCGTTGGGTTCGCTGt                                                                                                         >  2:2674378/1‑150 (MQ=255)
                                               tGATTACGCCACCAGCGCCATTGCATTTGGCAAAACCCGCGTCGCCTGGCATAAAGGCGTCCCCGTGCCGCCAGGTTGCCTGATTGACGTTAACGGCGTGCCTACGACCAATCCGGCGGTAATGCAGGAGTCGCCGTTGGGTTCGCTGtt                                                                                                        >  1:1795504/1‑150 (MQ=255)
                                               tGATTACGCCACCAGCGCCATTGCATTTGGCAAAACCCGCGTCGCCTGGCATAAAGGCGTCCCCGTGCCGCCAGGTTGCCTGATTGACGTTAACGGCGTGCCGACGACCAATCCGGCGGTAATGCAGGAGTCGCCGTTGGGTTCGCTGtt                                                                                                        <  1:3109519/150‑1 (MQ=255)
                                                gATTACGCCACCAGCGCCATTGCATTTGGCAAAACCCGCGTCGCCTGGCATAAAGGCGTCCCCGTGCCGCCAGGTTGCCTGATTGACGTTAACGGCGTGCCTACGACCAATCCGGCGGTAATGCAGGAGTCGCCGTTGGGTTCGCTGTTg                                                                                                       >  1:1050556/1‑150 (MQ=255)
                                                   tACGCCACCAGCGCCATTGCATTTGGCAAAACCCGCGTCGCCTGGCATAAAGGCGTCCCCGTGCCGCCAGGTTGCCTGATTGACGTTAACGGCGTGCCGACGACCAATCCGGCGGTAATGCAGGAGTCGCCGTTGGGTTCGCTGTTGAcc                                                                                                    >  2:3484264/1‑150 (MQ=255)
                                                        caccaGCGCCATTGCATTTGGCAAAACCCGCGTCGCCTGGCATAAAGGCGTCCCCGTGCCGCCAGGTTGCCTGATTGACGTTAACGGCGTGCCGACGACCAATCCGGCGGTAATGCAGGAGTCGCCGTTGGGTTCGCTGTTGACCTTTGc                                                                                               >  2:1085207/1‑150 (MQ=255)
                                                          ccaGCGCCATTGCATTTGGCAAAACCCGCGTCGCCTGGCATAAAGGCGTCCCCGTGCCGCCAGGTTGCCTGATTGACGTTAACGGCGTGCCGACGACCAATCCGGCGGTAATGCAGGAGTCGCCGTTGGGTTCGCTGTTGACCTTTGCCg                                                                                             >  1:3631980/1‑150 (MQ=255)
                                                           caGCGCCATTGCATTTGGCAAAACCCGCGTCGCCTGGCATAAAGGCGTCCCCGTGCCGCCAGGTTGCCTGATTGACGTTAACGGCGTGCCGACGACCAATCCGGCGGTAATGCAGGAGTCGCCGTTGGGTTCGCTGTTGACCTTTGCCGa                                                                                            <  1:1120153/150‑1 (MQ=255)
                                                               gcCATTGCATTTGGCAAAACCCGCGTCGCCTGGCATAAAGGCGTCCCCGTGCCGCCAGGTTGCCTGATTGACGTTAACGGCGTGCCTACGACCAATCCGGCGGTAATGCAGGAGTCGCCGTTGGGTTCGCTGTTGACCTTTGCCGAACAt                                                                                        >  2:504896/1‑150 (MQ=255)
                                                               gcCATTGCATTTGGCAAAACCCGCGTCGCCTGGCATAAAGGCGTCCCCGTGCCGCCAGGTTGCCTGATTGACGTTAACGGCGTGCCTACGACCAATCCGGCGGTAATGCAGGAGTCGCCGTTGGGTTCGCTGTTGACCTTTGCCGAACAt                                                                                        >  2:4410751/1‑150 (MQ=255)
                                                                ccATTGCATTTGGCAAAACCCGCGTCGCCTGGCATAAAGGCGTCCCCGTGCCGCCAGGTTGCCTGATTGACGTTAACGGCGTGCCGACGACCAATCCGGCGGTAATGCAGGAGTCGCCGTTGGGTTCGCTGTTGACCTTTGCCGAACATa                                                                                       <  1:2790176/150‑1 (MQ=255)
                                                                 cATTGCATTTGGCAAAACCCGCGTCGCCTGGCATAAAGGCGTCCCCGTGCCGCCAGGTTGCCTGATTGACGTTAACGGCGTGCCTACGACCAATCCGGCGGTAATGCAGGAGTCGCCGTTGGGTTCGCTGTTGACCTTTGCCGAACATaa                                                                                      >  1:1383987/1‑150 (MQ=255)
                                                                 cATTGCATTTGGCAAAACCCGCGTCGCCTGGCATAAAGGCGTCCCCGTGCCGCCAGGTTGCCTGATTGACGTTAACGGCGTGCCGACGACCAATCCGGCGGTAATGCAGGAGTCGCCGTTGGGTTCGCTGTTGACCTTTGCCGAACATaa                                                                                      <  1:2015760/150‑1 (MQ=255)
                                                                  aTTGCATTTGGCAAAACCCGCGTCGCCTGGCATAAAGGCGTCCCCGTGCCGCCAGGTTGCCTGATTGACGTTAACGGCGTGCCTACGACCAATCCGGCGGTAATGCAGGAGTCGCCGTTGGGTTCGCTGTTGACCTTTGCCGAACATaaa                                                                                     <  2:3857007/150‑1 (MQ=255)
                                                                  aTTGCATTTGGCAAAACCCGCGTCGCCTGGCATAAAGGCGTCCCCGTGCCGCCAGGTTGCCTGATTGACGTTAACGGCGTGCCGACGACCAATCCGGCGGTAATGCAGGAGTCGCCGTTGGGTTCGCTGTTGTCCTTTGCCGAACATaaa                                                                                     >  2:131863/1‑150 (MQ=255)
                                                                      cATTTGGCAAAACCCGCGTCGCCTGGCATAAAGGCGTCCCCGTGCCGCCAGGTTGCCTGATTGACGTTAACGGCGTGCCGACGACCAATCCGGCGGTAATGCAGGAGTCGCCGTTGGGTTCGCTGTTGACCTTTGCCGAACATAAAGGCt                                                                                 >  2:4694870/1‑150 (MQ=255)
                                                                       aTTTGGCAAAACCCGCGTCGCCTGGCATAAAGGCGTCCCCGTGCCGCCAGGTTGCCTGATTGACGTTAACGGCGTGCCTACGACCAATCCGGCGGTAATGCAGGAGTCGCCGTTGGGTTCGCTGTTGACCTTTGCCGAACATAAAGGCTa                                                                                >  2:705308/1‑150 (MQ=255)
                                                                       aTTTGGCAAAACCCGCGTCGCCTGGCATAAAGGCGTCCCCGTGCCGCCAGGTTGCCTGATTGACGTTAACGGCGTGCCGACGACCAATCCGGCGGTAATGCAGGAGTCGCCGTTGGGTTCGCTGTTGACCTTTGCCGAACATAAAGGCTa                                                                                >  2:1454346/1‑150 (MQ=255)
                                                                        tttGGCAAAACCCGCGTCGCCTGGCATAAAGGCGTCCCCGTGCCGCCAGGTTGCCTGATTGACGTTAACGGCGTGCCGACGACCAATCCGGCGGTAATGCAGGAGTCGCCGTTGGGTTCGCTGTTGACCTTTGCCGAACATAAAGGCTAc                                                                               <  2:4041706/150‑1 (MQ=255)
                                                                         ttGGCAAAACCCGCGTCGCCTGGCATAAAGGCGTCCCCGTGCCGCCAGGTTGCCTGATTGACGTTAACGGCGTGCCTACGACCAATCCGGCGGTAATGCAGGAGTCGCCGTTGGGTTCGCTGTTGACCTTTGCCGAACATAAAGGCTACg                                                                              >  2:2522594/1‑150 (MQ=255)
                                                                         ttGGCAAAACCCGCGTCGCCTGGCATAAAGGCGTCCCCGTGCCGCCAGGTTGCCTGATTGACGTTAACGGCGTGCCTACGACCAATCCGGCGGTAATGCAGGAGTCGCCGTGGGGTTCGCTGTTGACCTTTGCCGAACATAAAGGCTACg                                                                              >  2:2523011/1‑150 (MQ=255)
                                                                             cAAAACCCGCGTCGCCTGGCATAAAGGCGTCCCCGTGCCGCCAGGTTGCCTGATTGACGTTAACGGCGTGCCTACGACCAATCCGGCGGTAATGCAGGAGTCGCCGTTGGGTTCGCTGTTGACCTTTGCCGAACATAAAGGCTACGCCCt                                                                          >  1:2323805/1‑150 (MQ=255)
                                                                              aaaaCCCGCGTCGCCTGGCATAAAGGCGTCCCCGTGCCGCCAGGTTGCCTGATTGACGTTAACGGCGTGCCTACGACCAATCCGGCGGTAATGCAGGAGTCGCCGTTGGGTTCGCTGTTGACCTTTGCCGAACATAAAGGCTACGCCCtt                                                                         <  1:3859687/150‑1 (MQ=255)
                                                                              aaaaCCCGCGTCGCCTGGCATAAAGGCGTCCCCGTGCCGCCAGGTTGCCTGATTGACGTTAACGGCGTGCCGACGACCAATCCGGCGGTAATGCAGGAGTCGCCGTTGGGTTCGCTGTTGACCTTTGCCGAACATAAAGGCTACGCCCtt                                                                         <  1:950392/150‑1 (MQ=255)
                                                                               aaaCCCGCGTCGCCTGGCATAAAGGCGTCCCCGTGCCGCCAGGTTGCCTGATTGACGTTAACGGCGTGCCGACGACCAATCCGGCGGTAATGCAGGAGTCGCCGTTGGGTTCGCTGTTGACCTTTGCCGAACATAAAGGCTACGCCCTTg                                                                        >  1:1427594/1‑150 (MQ=255)
                                                                                    cgcgTCGCCTGGCATAAAGGCGTCCCCGTGCCGCCAGGTTGCCTGATTGACGTTAACGGCGTGCCGACGACCAATCCGGCGGTAATGCAGGAGTCGCCGTTGGGTTCGCTGTTGACCTTTGCCGAACATAAAGGCTACGCCCTTGCAGCg                                                                   <  1:2166415/150‑1 (MQ=255)
                                                                                    cgcgTCGCCTGGCATAAAGGCGTCCCCGTGCCGCCAGGTTGCCTGATTGACGTTAACGGCGTGCCGACGACCAATCCGGCGGTAATGCAGGAGTCGCCGTTGGGTTCGCTGTTGACCTTTGCCGAACATAAAGGCTACGCCCTTGCAGCg                                                                   <  1:3619408/150‑1 (MQ=255)
                                                                                      cgTCGCCTGGCATAACGGCGTCCCCGTGCCGCCAGGTGGCCTGATTGACGTTAACGGCGTGCCTACGACCAATCCGGCGGTAATGCAGGAGTCGCCGTTGGGTTCGCTGTTGACCTTTGCCGAACATAAAGGCTACGCCCTTGCAGCGAt                                                                 <  1:4946586/150‑1 (MQ=255)
                                                                                      cgTCGCCTGGCATAAAGGCGTCCCCGTGCCGCCAGGTTGCCTGATTGACGTTAACGGCGTGCCGACGACCAATCCGGCGGTAATGCAGGAGTCGCCGTTGGGTTCGCTGTTGACCTTTGCCGAACATAAAGGCTACGCCCTTGCAGCGAt                                                                 >  1:1624063/1‑150 (MQ=255)
                                                                                      cgTCGCCTGGCATAAAGGCGTCCCCGTGCCGCCAGGTTGCCTGATTGACGTTAACGGCGTGCCGACGACCAATCCGGCGGTAATGCAGGAGTCGCCGTTGGGTTCGCTGTTGACCTTTGCCGAACATAAAGGCTACGCCCTTGCAGCGAt                                                                 >  1:1624036/1‑150 (MQ=255)
                                                                                       gTCGCCTGGCATAAAGGCGTCCCCGTGCCGCCAGGTTGCCTGATTGACGTTAACGGCGTGCCTACGACCAATCCGGCGGTAATGCAGGAGTCGCCGTTGGGTTCGCTGTTGACCTTTGCCGAACATAAAGGCTACGCCCTTGCAGCGAtg                                                                <  2:3426764/150‑1 (MQ=255)
                                                                                             tGGCATAAAGGCGTCCCCGTGCCGCCAGGTTGCCTGATTGACGTTAACGGCGTGCCTACGACCAATCCGGCGGTAATGCAGGAGTCGCCGTTGGGTTCGCTGTTGACCTTTGCCGAACATAAAGGCTACGCCCTTGCAGCGATGTGTGac                                                          >  2:4612621/1‑149 (MQ=255)
                                                                                               gCATAAAGGCGTCCCCGTGCCGCCAGGTTGCCTGATTGACGTTAACGGCGTGCCTACGACCAATCCGGCGGTAATGCAGGAGTCGCCGTTGGGTTCGCTGTTGACCTTTGCCGAACATAAAGGCTACGCCCTTGCAGCGATGTGTGAAAt                                                        >  1:4849095/1‑150 (MQ=255)
                                                                                               gCAGAAAGGCGTCCCCGAGCCGCCAGGTTGCCTGATTGACGTTAACGGCGTGCCGACGACCAATCCGGCGGTAATGCAGGAGTCGCCGTTGGGTTCGCTGTTGACCTTTGCCGAACATAAAGGCTACGCCCTTGCAGCGATGTGTGAAAt                                                        <  2:4115454/150‑1 (MQ=255)
                                                                                                cATAAAGGCGTCCCCGTGCCGCCAGGTTGCCTGATTGACGTTAACGGCGTGCCGACGACCAATCCGGCGGTAATGCAGGAGTCGCCGTTGGGTTCGCTGTTGACCTTTGCCGAACATAAAGGCTACGCCCTTGCAGCGATGTGTGAAAtt                                                       >  2:4774621/1‑150 (MQ=255)
                                                                                                   aaaGGCGTCCCCGTGCCGCCAGGTTGCCTGATTGACGTTAACGGCGTGCCGACGACCAATCCGGCGGTAATGCAGGAGTCGCCGTTGGGTTCGCTGTTGACCTTTGCCGAACATAAAGGCTACGCCCTTGCAGCGATGTGTGAAATTCtt                                                    <  2:722137/150‑1 (MQ=255)
                                                                                                   aaaGGCGTCCCCGTGCCGCCAGGTTGCCTGATTGACGTTAACGGCGTGCCGACGACCAATCCGGCGGTAATGCAGGAGTCGCCGTTGGGTTCGCTGTTGACCTTTGCCGAACATAAAGGCTACGCCCTTGCAGCGATGTGTGAAATTCtt                                                    >  2:3447499/1‑150 (MQ=255)
                                                                                                    aaGGCGTCCCCGTGCCGCCAGGTTGCCTGATTGACGTTAACGGCGTGCCTACGACCAATCCGGCGGTAATGCAGGAGTCGCCGTTGGGTTCGCTGTTGACCTTTGCCGAACATAAAGGCTACGCCCTTGCAGCGATGTGTGAAATTCTTg                                                   <  2:4248799/150‑1 (MQ=255)
                                                                                                    aaGGCGTCCCCGTGCCGCCAGGTTGCCTGATTGACGTTAACGGCGTGCCGACGACCAATCCGGCGGTAATGCAGGAGTCGCCGTTGGGTTCGCTGTTGACCTTTGCCTAACATAAAGGCTACGCCCTTGCAGCGATGTGTGAAATTCTTg                                                   >  1:4018946/1‑150 (MQ=255)
                                                                                                        cGTCCCCGTGCCGCCAGGTTGCCTGATTGACGTTAACGGCGTGCCGACGACCAATCCGGCGGTAATGCAGGAGTCGCCGTTGGGTTCGCTGTTGACCTTTGCCGAACATAAAGGCTACGCCCTTGCAGCGATGTGTGAAATTCTTGGCgg                                               >  1:2899973/1‑150 (MQ=255)
                                                                                                        cGTCCCCGTGCCGCCAGGTTGCCTGATTGACGTTAACGGCGTGCCGACGACCAATCCGGCGGTAATGCAGGAGTCGCCGTTGGGTTCGCTGTTGACCTTTGCCGAACATAAAGGCTACGCCCTTGCAGCGATGTGTGAAATTCTTGGCgg                                               >  1:491587/1‑150 (MQ=255)
                                                                                                         gTCCCCGTGCCGCCAGGTTGCCTGATTGACGTTAACGGCGTGCCTACGACCAATCCGGCGGTAATGCAGGAGTCGCCGTTGGGTTCGCTGTTGACCTTTGCCGAACATAAAGGCTACGCCCTTGCAGCGATGTGTGAAATTCTTGGCggg                                              >  1:892372/1‑150 (MQ=255)
                                                                                                         gTCCCCGTGCCGCCAGGTTGCCTGATTGACGTTAACGGCGTGCCGACGACCAATCCGGCGGTAATGCAGGAGTCGCCGTTGGGTTCGCTGTTGACCTTTGCCGAACATAAAGGCTACGCCCTTGCAGCGATGTGTGAAATTCTTGGCggg                                              >  2:4470028/1‑150 (MQ=255)
                                                                                                          tCCCCGTGCCGCCAGGTTGCCTGATTGACGTTAACGGCGTGCCGACGACCAATCCGGCGGTAATGCAGGAGTCGCCGTTGGGTTCGCTGTTGACCTTTGCCGAACATAAAGGCTACGCCCTTGCAGCGATGTGTGAAATTCTTGGCgggg                                             >  2:2092872/1‑150 (MQ=255)
                                                                                                           ccccGTGCCGCCAGGTTGCCTGATTGACGTTAACGGCGTGCCGACGACCAATCCGGCGGTAATGCAGGAGTCGCCGTTGGGTTCGCTGTTGACCTTTGCCGAACATAAAGGCTACGCCCTTGCAGCGATGTGTGAAATTCTTGGCGGGgc                                            <  2:4508541/150‑1 (MQ=255)
                                                                                                             ccGTGCCGCCAGGTTGCCTGATTGACGTTAACGGCGTGCCTACGACCAATCCGGCGGTAATGCAGGAGTCGCCGTTGGGTTCGCTGTTGACCTTTGCCGAACATAAAGGCTACGCCCTTGCAGCGATGTGTGAAATTCTTGGCGGGgcgc                                          >  1:546209/1‑150 (MQ=255)
                                                                                                               gTGCCGCCAGGTTGCCTGATTGACGTTAACGGCGTGCCTACGACCAATCCGGCGGTAATGCAGGAGTCGCCGTTGGGTTCGCTGTTGACCTTTGCCGAACATAAAGGCTACGCCCTTGCAGCGATGTGTGAAATTCTTGGCGGGGCGCtt                                        <  2:1584048/150‑1 (MQ=255)
                                                                                                               gTGCCGCCAGGTTGCCTGATTGACGTTAACGGCGTGCCGACGACCAATCCGGCGGTAATGCAGGAGTCGCCGTTGGGTTCGCTGTTGACCTTTGCCGAACATAAAGGCTACGCCCTTGCAGCGATGTGTGAAATTCTTGGCGGGGCGCtt                                        >  1:4398142/1‑150 (MQ=255)
                                                                                                                  ccgccAGGTTGCCTGATTGACGTTAACGGCGTGCCTACGACCAATCCGGCGGTAATGCAGGAGTCGCCGTTGGGTTCGCTGTTGACCTTTGCCGAACATAAAGGCTACGCCCTTGCAGCGATGTGTGAAATTCTTGGCGGGGCGCTTTcc                                     >  2:114504/1‑150 (MQ=255)
                                                                                                                   cgccAGGTTGCCTGATTGACGTTAACGGCGTGCCGACGACCAATCCGGCGGTAATGCAGGAGTCGCCGTTGGGTTCGCTGTTGACCTTTGCCGAACATAAAGGCTACGCCCTTGCAGCGATGTGTGAAATTCTTGGCGGGGCGCTTTCcg                                    <  2:3557583/150‑1 (MQ=255)
                                                                                                                      cAGGTTGCCTGATTGACGTTAACGGCGTGCCGACGACCAATCCGGCGGTACTGCAGGAGTCGCCGTTGGGTTCGCTGTTGACCTTTGCCGAACATAAAGGCTACGCCCTTGCAGCGATGTGTGAAATTCTTGGCGGGGCGCTTTCcggcg                                 >  2:2001964/1‑150 (MQ=255)
                                                                                                                       aGGTTGCCTGATTGACGTTAACGGCGTGCCGACGACCAATCCGGCGGTAATGCAGGAGTCGCCGTTGGGTTCGCTGTTGACCTTTGCCGAACATAAAGGCTACGCCCTTGCAGCGATGTGTGAACTTCTTGGCGGGGCGCTTTCcggcgg                                >  2:2841560/1‑150 (MQ=255)
                                                                                                                        ggTTGCCTGATTGACGTTAACGGCGTGCCTACGACCAATCCGGCGGTAATGCAGGAGTCGCCGTTGGGTTCGCTGTTGACCTTTGCCGAACATAAAGGCTACGCCCTTGCAGCGATGTGTGAAATTCTTGGCGGGGCGCTTTCCGGCGGt                               >  1:4743389/1‑150 (MQ=255)
                                                                                                                        ggTTGCCTGATTGACGTTAACGGCGTGCCGACGACCAATCCGGCGGTAATGCAGGAGTCGCCGTTGGGTTCGCTGTTGACCTTTGCCGAACATAAAGGCTACGCCCTTGCAGCGATGTGTGAAATTCTTGGCGGGGCGCTTTCCGGCGGt                               >  1:4157954/1‑150 (MQ=255)
                                                                                                                          ttGCCTGATTGACGTTAACGGCGTGCCTACGACCAATCCGGCGGTAATGCAGGAGTCGCCGTTGGGTTCGCTGTTGACCTTTGCCGAACATAAAGGCTACGCCCTTGCAGCGATGTGTGAAATTCTTGGCGGGGCGCTTTCCGGCGGTaa                             <  2:318743/150‑1 (MQ=255)
                                                                                                                           tGCCTGATTGACGTTAACGGCGTGCCGACGACCAATCCGGCGGTAATGCAGGAGTCGCCGTTGGGTTCGCTGTTGACCTTTGCCGAACATAAAGGCTACGCCCTTGCAGCGATGTGTGAAATTCTTGGCGGGGCGCTTTCCGGCGGTaaa                            <  1:3692954/150‑1 (MQ=255)
                                                                                                                            gCCTGATTGACGTTAACGGCGTGCCGACGACCAATCCGGCGGTAATGCAGGAGTCGCCGTTGGGTTCGCTGTTGACCTTTGCCGAACATAAAGGCTACGCCCTTGCAGCGATGTGTGAAATTCTTGGCGGGGCGCTTTCCGGCGGTaaaa                           <  1:4321957/150‑1 (MQ=255)
                                                                                                                            gCCTGATTGACGTTAACGGCGTGCCGACGACCAATCCGGCGGTAATGCAGGAGTCGCCGTTGGGTTCGCTGTTGACCTTTGCCGAACATAAAGGCTACGCCCTTGCAGCGATGTGTGAAATTCTTGGCGGGGCGCTTTCCGGCGGTaaaa                           <  1:329967/150‑1 (MQ=255)
                                                                                                                                gATTGACGTTAACGGCGTGCCGACGACCAATCCGGCGGTAATGCAGGAGTCGCCGTTGGGTTCGCTGTTGACCTTTGCCGAACATAAAGGCTACGCCCTTGCAGCGATGTGTGAAATTCTTGGCGGGGCGCTTTCCGGCGGTAAAacgac                       <  1:456748/150‑1 (MQ=255)
                                                                                                                                   tGACGTTAACGGCGTGCCGACGACCAATCCGGCGGTAATGCAGGAGTCGCCGTTGGGTTCGCTGTTGACCTTTGCCGAACATAAAGGCTACGCCCTTGCAGCGATGTGTGAAATTCTTGGCGGGGCGCTTTCCGGCGGTAAAACGACGCa                    >  1:64409/1‑150 (MQ=255)
                                                                                                                                     aCGTTAACGGCGTGCCGACGACCAATCCGGCGGTAATGCAGGAGTCGCCGTTGGGTTCGCTGTTGACCTTTGCCGAACATAAAGGCTACGCCCTTGCAGCGATGTGTGAAATTCTTGGCGGGGCGCTTTCCGGCGGTAAAACGACGCATc                  <  1:3467958/150‑1 (MQ=255)
                                                                                                                                      cGTTACCGGCGTGCCGACGACCAATCCGGCGGTAATGCAGGAGTCGCCGTTGGGTTCGCTGTTGACCTTTGCCGAACATAAAGGCTACGCCCTTGCAGCGATGTGTGAAATTCTTGGCGGGGCGCTTTCCGGCGGTAAAACGACGCATCa                 <  1:1850532/150‑1 (MQ=255)
                                                                                                                                           aCGGCGTGCCGACGACCAATCCGGCGGTAATGCAGGAGTCGCCGTTGGGTTCGCTGTTGACCTTTGCCGAACATAAAGGCTACGCCCTTGCAGCGATGTGTGAAATTCTTGGCGGGGCGCTTTCCGGCGGTAAAACGACGCATCAGGaaa            <  1:4870195/150‑1 (MQ=255)
                                                                                                                                             ggCGTGCCGACGACCAATCCGGCGGTAATGCAGGAGTCGCCGTTGGGTTCGCTGTTGACCTTTGCCGAACATAAAGGCTACGCCCTTGCAGCGATGTGTGAAATTCTTGGCGGGGCGCTTTCCGGCGGTAAAACGACGCATCAGGAAACg          >  1:260012/1‑150 (MQ=255)
                                                                                                                                             ggCGTGCCGACGACCAATCCGGCGGTAATGCAGGAGTCGCCGTTGGGTTCGCTGTTGACCTTTGCCGAACATAAAGGCTACGCCCTTGCAGCGATGTGTGAAATTCTTGGCGGGGCGCTTTCCGGCGGTAAAACGACGCATCAGGAAACg          >  2:4578625/1‑150 (MQ=255)
                                                                                                                                               cGTGCCGACGACCAATCCGGCGGTAATGCAGGAGTCGCCGTTGGGTTCGCTGTTGACCTTTGCCGAACATAAAGGCTACGCCCTTGCAGCGATGTGTGAAATTCTTGGCGGGGCGCTTTCCGGCGGTAAAACGACGCATCAGGAAACGtt        >  2:1733846/1‑150 (MQ=255)
                                                                                                                                                gTGCCTACGACCAATCCGGCGGTAATGCAGGAGTCGCCGTTGGGTTCGCTGTTGACCTTTGCCGAACATAAAGGCTACGCCCTTGCAGCGATGTGTGAAATTCTTGGCGGGGCGCTTTCCGGCGGTAAAACGACGCATCAGGAAACGTTa       >  1:996063/1‑150 (MQ=255)
                                                                                                                                                gTGCCGACGACCAATCCGGCGGTAATGCAGGAGTCGCCGTTGGGTTCGCTGTTGACCTTTGCCGAACATAAAGGCTACGCCCTTGCAGCGATGTGTGAAATTCTTGGCGGGGCGCTTTCCGGCGGTAAAACGACGCATCAGGAAACGTTa       >  2:3025146/1‑150 (MQ=255)
                                                                                                                                                  gCCGACGACCAATCCGGCGGTAATGCAGGAGTCGCCGTTGGGTTCGCTGTTGACCTTTGCCGAACATAAAGGCTACGCCCTTGCAGCGATGTGTGAAATTCTTGGCGGGGCGCTTTCCGGCGGTAAAACGACGCATCAGGAAACGTTACa     >  2:2863029/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CCGTTCTGTGTGGTTTTCCCTCGTAAAGATAATTTCCCGCTGTTGCTTGATTACGCCACCAGCGCCATTGCATTTGGCAAAACCCGCGTCGCCTGGCATAAAGGCGTCCCCGTGCCGCCAGGTTGCCTGATTGACGTTAACGGCGTGCCGACGACCAATCCGGCGGTAATGCAGGAGTCGCCGTTGGGTTCGCTGTTGACCTTTGCCGAACATAAAGGCTACGCCCTTGCAGCGATGTGTGAAATTCTTGGCGGGGCGCTTTCCGGCGGTAAAACGACGCATCAGGAAACGTTACAAAC  >  CP009273/832296‑832594

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑