Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,118,506 A→T 6.0% L75Q (CTA→CAA)  yceB ← lipoprotein, DUF1439 family

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,118,5060AT 6.0% 803.3 / 3.1 261L75Q (CTA→CAA) yceBlipoprotein, DUF1439 family
Reads supporting (aligned to +/- strand):  ref base A (115/121);  new base T (3/19);  total (118/143)
Fisher's exact test for biased strand distribution p-value = 1.41e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 263 total aligned reads displayed.

GTTGTACCGTCGCATCGACCACTTCCATCTCTTTCAGGAAGATCGCACCTTTTTCTTTATCAAACACGGGCAGCGCTTTCAGCTTCAGTTTCATGGTCGCTTTCTGACTACCGAACAGGGAGTTCATGTCCAGATTGGCGTCTCCGGTTAGGGTAACCTTATTCGGCTCTTCGCGACCAATTTGGCTGGTCAGGTTTGTCAGAACAATATGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATGGT  >  CP009273/1118357‑1118654
                                                                                                                                                     |                                                                                                                                                    
     aCCGTCGCATCGACCACTTCCATCTCTTTCAGGAAGATCGCACCTTTTTCTTTATCAAACACGGGCAGCGCTTTCAGCTTCAGTTTCATGGTCGCTTTCTGACTACCGAACAGGGAGTTCATGTCCAGATTGGCGTCTCCGGTTAGGGTa                                                                                                                                                 <  1:1574087/150‑1 (MQ=255)
      ccGTCGCATCGACCACTTCCATCTCTTTCAGGAAGATCGCACCTTTTTCTTTATCAAACACGGGCAGCGCTTTCAGCTTCAGTTTCATGGTCGCTTTCTGACTACCGAACAGGGAGTTCATGTCCAGATTGGCGTCTCCGGTTAGGGTaa                                                                                                                                                >  1:4657413/1‑150 (MQ=255)
       cGTCGCATCGACCACTTCCATCTCTTTCAGGAAGATCGCACCTTTTTCTTTATCAAACACGGGCAGCGCTTTCAGCTTCAGTTTCATGGTCGCTTTCTGACTACCGAACAGGGAGTTCATGTCCAGATTGGCGTCTCCGGTTAGGGTAAc                                                                                                                                               >  2:381703/1‑150 (MQ=255)
       cGTCGCATCGACCACTTCCATCTCTTTCAGGAAGATCGCACCTTTTTCTTTATCAAACACGGGCAGCGCTTTCAGCTTCAGTTTCATGGTCGCTTTCTGACTACCGAACAGGGAGTTCATGTCCAGATTGGCGTCTCCGGTTAGGGTAAc                                                                                                                                               <  2:2099926/150‑1 (MQ=255)
       cGTCGCATCGACCACTTCCATCTCTTTCAGGAAGATCGCACCTTTTTCTTTATCAAACACGGGCAGCGCTTTCAGCTTCAGTTTCATGGTCGCGTTATGAATACCCAACAGGGCGTTCATGTCCAAATTTTCGTCTCCGGTTATGGTaaa                                                                                                                                               >  1:1342628/1‑149 (MQ=255)
        gTCGCATCGACCACTTCCATCTCTTTCAGGAAGATCGCACCTTTTTCTTTATCAAACACGGGCAGCGCTTTCAGCTTCAGTTTCATGGTCGCTTTCTGACTACCGAACAGGGAGTTCATGTCCAGATTTGCGTCTCCGGTTAGGGTAAcc                                                                                                                                              >  1:4994587/1‑150 (MQ=255)
         tCGCATCGTCCACTTCCATCTCTTTCAGGAAGATCGCACCTTTTTCTTTATCAAACACGGGCAGCGCTTTCAGCTTCAGTTTCATGGTCGCTTTCTGACTACCGAACAGGGAGTTCATGTCCAGATTGGCGTCTCCGGTTAGGGTAACCt                                                                                                                                             >  1:3536882/1‑150 (MQ=255)
           gCATCGACCACTTCCATCTCTTTCAGGAAGATCGCACCTTTTTCTTTATCAAACACGGGCAGCGCTTTCAGCTTCAGTTTCATGGTCGCTTTCTGACTACCGACCAGGGAGTTCATGTCCAGATTGGCGTCTCCGGTTAGGGTAACCTTa                                                                                                                                           >  2:5053173/1‑150 (MQ=255)
           gCATCGACCACTTCCATCTCTTTCAGGAAGATCGCACCTTTTTCTTTATCAAACACGGGCAGCGCTTTCAGCTTCAGTTTCATGGTCGCTTTCTGACTACCGAACAGGGGGTTCATGTCCAGATTTGCGTCTCCGGTTAGGGTAACCTTa                                                                                                                                           >  2:1310412/1‑150 (MQ=255)
            cATCGACCACTTCCATCTCTTTCAGGAAGATCGCACCTTTTTCTTTATCAAACACGGGCAGCGCTTTCAGCTTCAGTTTCATGGTCGCTTTCTGACTACCGACCAGGGAGTTCATTTCCAGATTGGCGTCTCCGGTTAGGGTAACCGTAt                                                                                                                                          >  2:606178/1‑150 (MQ=255)
            cATCGACCACTTCCATCTCTTTCAGGAAGATCGCACCTTTTTCTTTATCAAACACGGGCAGCGCTTTCAGCTTCAGTTTCATGGTCGCTTTCTGACTACCGAACAGGGAGTTCATGTCCAGATTGGCGTCTCCGGTTAGGGTAACCTTAt                                                                                                                                          <  2:563565/150‑1 (MQ=255)
            cATCGACCACTTCCATCTCTTTCAGGAAGATCGCACCTTTTTCTTTATCAAACACGGGCAGCGCTTTCAGCTTCAGTTTCATGGTCGCTTTCTGACTACCGAACAGGGAGTTCATGTCCAGATTGGCGTCTCCGGTTAGGGTAACCTTAt                                                                                                                                          >  1:2800726/1‑150 (MQ=255)
               cGACCACTTCCATCTCTTTCAGGAAGATCGCACCTTTTTCTTTATCAAACACGGGCAGCGCTTTCAGCTTCAGTTTCATGGTCGCTTTCTGACTACCGAACAGGGAGTTCATGTCCAGATTGGCGTCTCCGGTTAGGGTAACCTTATTCg                                                                                                                                       <  1:2969791/150‑1 (MQ=255)
                   cACTTCCATCTCTTTCAGGAAGATCGCACCTTTTTCTTTATCAAACACGGGCAGCGCTTTCAGCTTCAGTTTCATGGTCGCTTTCTGACTACCGAACAGGGAGTTCATGTCCAGATTGGCGTCTCCGGTTAGGGTAACCTTATTCGGctc                                                                                                                                   >  2:1365964/1‑150 (MQ=255)
                    aCTTCCATCTCTTTCAGGAAGATCGCACCTTTTTCTTTATCAAACACGGGCAGCGCTTTCAGCTTCAGTTTCATGGTCGCTTTCTGACTAACGAACAGGGAGTTCATGTCCAGATTTGCGTCTCCGGTTAGGGTAACCTTATTAGGctct                                                                                                                                  >  2:88945/1‑150 (MQ=255)
                      ttCCATCTCTTTCAGGAAGATCGCACCTTTTTCTTTATCAAACACGGGCAGCGCTTTCAGCTTCAGTTTCATGGTCGCTTTCTGACTACCGAACAGGGAGTTCATGTCCAGATTGGCGTCTCCGGTTAGGGTAACCTTATTCGGCTCTTc                                                                                                                                <  2:2845849/150‑1 (MQ=255)
                      ttCCATCTCTTTCAGGAAGATCGCACCTTTTTCTTTATCAAACACGGGCAGCGCTTTCAGCTTCAGTTTCATGGTCGCTTTCTGACTACCGAACAGGGAGTTCATGTCCAGATTGGCGTCTCCGGTTAGGGTAACCTTATTCGGCTCTTc                                                                                                                                <  2:3708140/150‑1 (MQ=255)
                           tctcTTTCAGGAAGATCGCACCTTTTTCTTTATCAAACACGGGCAGCGCTTTCAGCTTCAGTTTCATGGTCGCTTTCTGACTACCGAACAGGGAGTTCATGTCCAGATTGGCGTCTCCGGTTAGGGTAACCTTATTCGGCTCTTCGCGAc                                                                                                                           <  2:754436/150‑1 (MQ=255)
                            ctctTTCAGGAAGATCGCACCTTTTTCTTTATCAAACACGGGCAGCGCTTTCAGCTTCAGTTTCATGGTCGCTTTATGACTAACGAACAGGGAGTTGATGTACAGATTTGGGTCGCCGGGTTGGGGAACCTTATTAGGCGCTTCGCGAcc                                                                                                                          >  1:105325/1‑150 (MQ=255)
                                ttCAGGAAGATCGCACCTTTTTCTTTATCAAACACGGGCAGCGCTTTCAGCTTCAGTTTCATGGTCGCTTTCTGACTACCGAACAGGGAGTTCATGTCCAGAGTGGCGTCGCCGGGTAGGGGAACCTTATTCGGCGCTTCGCGACCCAtt                                                                                                                      >  2:1818461/1‑150 (MQ=255)
                                  cAGGAAGATCGCACCTTTTTCTTTATCAAACACGGGCAGCGCTTTCAGCTTCAGTTTCATGGTCGCTTTCTGACTACCGAACAGGGAGTTCATGTCCAGATTGGCGTCTCCGGTTAGGGTAACCTTATTCGGCTCTTCGCGACCAATTTg                                                                                                                    <  1:705809/150‑1 (MQ=255)
                                      aaGATCGCACCTTTTTCTTTATCAAACACGGGCAGCGCTTTCAGCTTCAGTTTCATGGTCGCTTTCTGACTACCGAACAGGGAGTTCATGTCCAGATTGGCGTCTCCGGTTAGGGTAACCTTATTCGGCTCTTCGCGACCAATTTGGCTg                                                                                                                <  1:2599298/150‑1 (MQ=255)
                                           cGCACCTTTTTCTTTATCAAACACGGGCAGCGCTTTCAGCTTCAGTTTCATGGTCGCTTTCTGACTACCGCACAGGGGGTTGAAGTCCACATTTGCGTCGCCGGGTAGGGGAAACGTATTAGGCTCTTCGCGACCAAATTGGCTgggccg                                                                                                           >  1:906599/1‑146 (MQ=255)
                                           cGCACCTTTTTCTTTATCAAACACGGGCAGCGCTTTCAGCTTCAGTTTCATGGTCGCTTTCTGACTACCGAACAGGGAGTTCATGTCCAGATTGGCGTCTCCGGTTAGGGTAACCTTATTCGGCTCTTCGCGACCAATTTGGCTGGTCAg                                                                                                           >  2:3378835/1‑150 (MQ=255)
                                           cGCACCTTTTTCTTTATCAAACACGGGCAGCGCTTTCAGCTTCAGTTTCATGGTCGCTTTCTGACTACCGAACAGGGAGTTCATGTCCAGATTGGCGTCTCCGGTTAGGGTAACCTTATTCGGCTCTTCGCGACCAATTTGGCTGGTCAg                                                                                                           >  2:4345738/1‑150 (MQ=255)
                                           cGCACCTTTTTCTTTATCAAACACGGGCAGCGCTTTCAGCTTCAGTTTCATGGTCGCTTTATGACTACCGAACAGGGAGTTAATGTCCAGATTGGCGTCTCCGGTTAGGGTAACCTTATTAGGCTCTTCGCGACCAATTTGGCTGGTCAg                                                                                                           >  2:471/1‑150 (MQ=255)
                                            gCACCTTTGTCTTTATCAAACACGGGCAGCGCTTTCAGCTTCAGTTTCATGGTCGCTTTCTGACTACCGAACAGGGAGTTCATGTCCAGATTGGCGTCTCCGGTTAGGGTAACCTTATTCGGCTCTTCGCGACCAATTTGGCTGGTCAgg                                                                                                          <  2:2695144/150‑1 (MQ=255)
                                             cACCTTTTTCATTATCAAACACGGGCAGCGCTTTCAGCTTCAGTTTCATGGTCGCTTTCTGACTACCGAACAGGGAGTTCATGTCCAGATTGGCGTCTCAGGTTAGGGTAAACTTATTCGGCTCTTCGCGACCAATTTGGCTGGTAAggg                                                                                                         >  2:2900322/1‑149 (MQ=255)
                                              aCCTTTTTCTTTATCAAACACGGGCAGCGCTTTCAGCTTCAGTTTCATGGTCGCTTTCTGACTACCGAACAGGGAGTTCATGTCCAGATTGGCGTCTCCGGTTAGGGTAACCTTATTCGGCTCTTCGCGACCAATTTGGCTGGTCAGGtt                                                                                                        <  2:4594298/150‑1 (MQ=255)
                                              aCCTTTTTCTTTATCAAACACGGGCAGCGCTTTCAGCTTCAGTTTCATGGTCGCTTTCTGACTACCGAACAGGGAGTTCATGTCCAGATTGGCGTCTCCGGTTAGGGTAACCTTATTCGGCTCTTCGCGACCAATTTGGCTGGTCAGGtt                                                                                                        <  1:3953768/150‑1 (MQ=255)
                                              aCCTTTTTCTTTATCAAACACGGGCAGCGCTTTCAGCTTCAGTTTCATGGTCGCTTTCTGACTACCGAACAGGGAGTTCATGTCCAGATTGGCGTCTCCGGTTAGGGTAACCTTATTCGGCTCTTCGCGACCAATTTGGCTGGTCAGGtt                                                                                                        <  1:4644590/150‑1 (MQ=255)
                                               ccTTTTTCTTTCTCAAACACGGGCAGCGCTTTCAGCTTCAGTTTCATGGTCGCTTTCTGACTACCGAACAGGGAGTTCATGTCCAGATTGGCGTCTCCGGTTAGGGTAACCTTATTCGGCTCTTCGCGACCAATTTGGCTGGTCAGGttt                                                                                                       <  1:3067564/150‑1 (MQ=255)
                                               ccTTTTTCTTTATCAAACACGGGCAGCGCTTTCAGCTTCAGTTTCATGGTCGCTTTCTGACTACCGAACAGGGAGTTCATGTCCAGATTGGCGTCTCCGGTTAGGGTAACCTTATTCGGCTCTTCGCGACCAATTTGGCTGGTCAGGttt                                                                                                       <  1:3067497/150‑1 (MQ=255)
                                                       tttATCAAACACGGGCAGCGCTTTCAGCTTCAGTTTCATGGTCGCTTTCTGACTACCGAACAGGGAGTTCATGTCCAGATTGGCGTCTCCGGTTAGGGTAACCTTATTCGGCTCTTCGCGACCAATTTTGCTGGTCAGGTTTGTCAGAAc                                                                                               >  2:1426687/1‑150 (MQ=255)
                                                         tATCAAACACGGGCAGCGCTTTCAGCTTCAGTTTCATGGTCGCTTTCTGACTACCGAACAGGGAGTTCATGTCCAGATTGGCGTCTCCGGTTAGGGTAACCTTATTCGGCTCTTCGCGACCAATTTGGCTGGTCAGGTTTGTCAGAACaa                                                                                             >  2:3980562/1‑150 (MQ=255)
                                                           tCAAACACGGGCAGCTCTTTCAGCTTCAGTTTCATGGTCGCTTTCTGACTACCGAACAGGGAGTTCATGTCCAGATTGGCGTCTCCGGTTTGGGTAACCTTATTCGGCTCTTCGCGACCAATTTGGCTGGTCAGGTTTGTCAGAACAata                                                                                           <  1:4386589/150‑1 (MQ=255)
                                                              aaCACGGGCAGCGCTTTCAGCTTCAGTTTCATGGTCGCTTTCTGACTACCGAACAGGGAGTTCATGTCCAGATTGGCGTCTCCGGTTAGGGTAACCTTATTCGGCTCTTCGCGACCAATTTGGCTGGTCAGGTTTGTCAGAACAATATgg                                                                                        <  1:2640162/150‑1 (MQ=255)
                                                              aaCACGGGCAGCGCTTTCAGCTTCAGTTTCATGGTCGCTTTCTGACTACCGAACAGGGAGTTCATGTCCAGATTGGCGTCTCCGGTTAGGGTAACCTTATTCGGCTCTTCGCGACCAATTTGGCTGGTCAGGTTTGTCAGAACAATATgg                                                                                        <  2:820003/150‑1 (MQ=255)
                                                              aaCACGGGCAGCGCTTTCAGCTTCAGTTTCATGGTCGCTTTCTGACTACCGAACAGGGAGTTCATGTCCAGATTGGCGTCTCCGGTTAGGGTAACCTTATTCGGCTCTTCGCGACCAATTTGGCTGGTCAGGTTTGTCAGAACAATATgg                                                                                        <  1:1781631/150‑1 (MQ=255)
                                                              aaCACGGGCAGCGCTTTCAGCTTCAGTTTCATGGTCGCTTTCTGACTACCGAACAGGGAGTTCATGTCCAGATTGGCGTCTCCGGTTAGGGTAACCTTATTCGGCTCTTCGCGACCAATTTGGCTGGTCAGGTTTGTCAGAACAATATgg                                                                                        <  2:3423663/150‑1 (MQ=255)
                                                                cacGGGCAGCGCTTTCAGCTTCAGTTTCATGGTCGCTTTCTGACTACCGAACAGGGAGTTCATGTCCAGATTGGCGTCTCCGGTTAGGGTAACCTTATTCGGCTCTTCGCGACCAATTTGGCTGGTCAGGTTTGTCAGAACAATATGGGc                                                                                      <  2:1685614/150‑1 (MQ=255)
                                                                cacGGGCAGCGCTTTCAGCTTCAGTTTCATGGTCGCTTTCTGACTACCGAACAGGGAGTTCATGTCCAGATTGGCGTCTCCGGTTAGGGTAACCTTATTAGGCTCTTCGCGACCAATTTGGCTGGTCAGGTTTGTCAGAACAATATGGGc                                                                                      >  2:560557/1‑150 (MQ=255)
                                                                cacGGGCAGCGCTTTCAGCTTCAGTTTCATGGTCGCTTTCTGACTACCGAACAGGGAGTTCAAGTCCAGATTTGCGTCTCCGGTTTGGGTAACCATATTCGGCGCGTCGCGACCAAATTGGCTGGTCAGGGTTTTGAGAACAATATTGGc                                                                                      >  1:1914092/1‑150 (MQ=255)
                                                                 acGGGCAGCGCTTTCAGCTTCAGTTTCATGGTCGCTTTCTGACTACCGAACAGGGAGTTCATGTCCAGATTGGCGTCTCCGGTTAGGGTAACCTTATTCGGCTCTTCGCGACCAATTTGGCTGGTCAGGTTTGTCAGAACAATATGGGCg                                                                                     <  2:3226466/150‑1 (MQ=255)
                                                                 acGGGCAGCGCTTTCAGCTTCAGTTTCATGGTCGCTTTCTGACTACCGAACAGGGAGTTCATGTCCAGATTGGCGTCTCCGGTTAGGGTAACCTTATTCGGCTCTTCGCGACCAATTTGGCTGGTCAGGTTTGTCAGAACAATATGGGCg                                                                                     <  2:3461175/150‑1 (MQ=255)
                                                                  cGGGCAGCGCTTTCAGCTTCAGTTTCATGGTCGCTTTCTGACTACCGAACAGGGAGTTCATGTCCAGATTGGCGTCTCCGGTTAGGGTAACCTTATTCGGCTCTTCGCGACCAATTTGGCTGGTCAGGTTTGTCAGAACAATATGGGCGt                                                                                    <  2:1918367/150‑1 (MQ=255)
                                                                      cAGCGCTTTCAGCTTCAGTTTCATGGTCGCTTTATGACTACCCAACAGGGAGTTCATGTCCAGATTGGCGTCTCCGGTTAGGGTAACCTTATTAGGCTAGTCGCGACCAATTTGGCTGGTCAGGTTTGTCAGAACAATATGGGCGTCAGc                                                                                >  1:3541908/1‑150 (MQ=255)
                                                                       aGCGCTTTCAGCTTCAGTTTCATGGTCGCTTTCTGACTACCGAACAGGGAGTTCATGTCCAGATTTGCGTCTCCGGTTAGGGTAACCTTATTCGGCTCTTCGCGACCAATTTGGCTGGTCAGGTTTGTCAGAACAATATGGGCGTCAGcc                                                                               >  2:4148286/1‑150 (MQ=255)
                                                                         cgcTTTCAGCTTCAGTTTCATGGTCGCTTTCTGACTACCGAACAGGGAGTTCATGTCCAGATTGGCGTCTCCGGTTAGGGTAACCTTATTCGGCTCTTCGCGACCAATTTGGCTGGTCAGGTTTGTCAGAACAATATGGGCGTCAGCCAc                                                                             <  1:4296083/150‑1 (MQ=255)
                                                                         cgcTTTCAGCTTCAGTTTCATGGTCGCTTTCTGACTACCGAACAGGGAGTTCATGTCCAGATTGGCGTCTCCGGTTAGGGTAACCTTATTCGGCTCTTCGCGACCAATTTGGCTGGTCAGGTTTGTCAGAACAATATGGGCGTCAGCCAc                                                                             <  2:4472957/150‑1 (MQ=255)
                                                                         cgcTTTCAGCTTCAGTTTCATGGTCGCTTTCTGACTACCGAACAGGGAGTTCATGTCCAGATTGGCGTCTCCGGTTAGGGTAACCTTATTCGGCTCTTCGCGACCAATTTGGCTGGTCAGGTTTGTCAGAACAATATGGGCGTCAGCCAc                                                                             <  1:5148945/150‑1 (MQ=255)
                                                                          gcTTTCAGCTTCAGTTTCATGGTCGCTTTATGACTACCGAACAGGGCGTTCATGTCCAGATTTGCGTCTCCCGTTAGGGTAACCTTATTAGGCTCTTCGCGACCAATTTGGCTGGTCAGGTTTGTCAGAACAATATGGGCGTCAGCCACg                                                                            >  1:2481568/1‑150 (MQ=255)
                                                                           cTTTCAGCTTCAGTTTCATGGTCGCTTTCTGACTACCGAACAGGGAGTTCATGTCCAGATTGGCGTCTCCGGTTAGGGTAACCTTATTCGGCTCTTCGCGACCAAATTGGCGGGTCAGGTTTGTCAGAACAATATGGGCGTCAGCCACGc                                                                           >  1:3244035/1‑150 (MQ=255)
                                                                            tttCAGCTTCAGTTTCATGGTCGCTTTCTGACTACCGAACAGGGAGTTCATGTCCAGATTGGCGTCTCCGGTTAGGGTAACCTTATTCGGCTCTTCGCGACCAATTTGGCTGGTCAGGTTTGTCAGAACAATATGGGCGTCAGCCACGcc                                                                          <  1:2153840/150‑1 (MQ=255)
                                                                             ttCAGCTTCAGTTTCATGGTCGCTTTCTGACTACCGAACAGGGAGTTCATGTCCAGATTGGCGTCTCCGGTTAGGGTAACCTTATTCGGCTCTTCGCGACCAATTTGGCTGGTCAGGTTTGTCAGAACAATATGGGCGTCAGCCACGCCg                                                                         <  2:2518492/150‑1 (MQ=255)
                                                                             ttCAGCTTCAGTTTCATGGTCGCTTTCTGACTACCGAACAGGGAGTTCATGTCCAGATTGGCGTCTCCGGTTAGGGTAACCTTATTCGGCTCTTCGCGACCAATTTGGCTGGTCAGGTTTGTCAGAACAATATGGGCGTCAGCCACGCCg                                                                         >  2:2609620/1‑150 (MQ=255)
                                                                             ttCAGCTTCAGTTTCATGGTCGCTGTCTGACTACCGAACAGGGAGTTCATGTCCAGATTGGCGTCTCCGGTTAGGGTAACCTTATTCGGCTCTTCGCGACCAATTTGGCTGGTCAGGTTTGTCAGAACAATATGGGCGTCAGCCACGCCg                                                                         <  2:2881980/150‑1 (MQ=255)
                                                                               cAGCTTCAGTTTCATGGTCGCTTTATGTCTACCCAACAGGGAGTTGATGTCCAGATTTGCGTCTCCCGTTATGGTAACCGTATTAGGCGCTTAGCGACCAATTTTGCTGGTCAGGTTTGTAAGAACAATATTGGCGTAAGCCCCGCCggg                                                                       >  1:1918363/1‑150 (MQ=255)
                                                                                 gCTTCAGTTTCATGGTCGCTTTCTGACTACCGAACAGGGAGTTCATGTCCAGATTGGCGTCTCCGGTTAGGGTAACCTTATTCGGCTCTTCGCGACCAATTTGGCTGGTCAGGTTTGTCAGAACAATATGGGCGTCAGCCACGCCGGGTa                                                                     <  2:3100845/150‑1 (MQ=255)
                                                                                      aGTTTCATGGTCGCTTTATGACTACCGAACAGGGAGTTCATGTCCAGATTGGCGTCTCCGGTTAGGGTAACCTTATTCGGCTCTTCGCGACCAATTTGGCTGGTCAGGTTTGTCAGAACAATATGGGCGTCAGCCACGCCGGGTAAACCg                                                                >  2:294376/1‑150 (MQ=255)
                                                                                       gTTTCATGGTCGCTTTCTGAAAAACCAAAAGGGAGTTAAAGTCCACAATTTGGTAGCCCGTTAGGGTAAACATAAAATGCTCGTCGCGAACAAATTTGATGGTAAGGTTTTTCAAAAAAAAATGGGCGTCAGCCACCCCCGGTaaaacca                                                               >  1:2353653/1‑146 (MQ=255)
                                                                                        tttCATGGTCGCTGTCTGACTACCGAACAGGGAGTTCATGTCCAGATTGGCGTCTCCGGTTAGGGTAACCTTATTCGGCTCTTCGCGACCAATTTGGCTGGTCAGGTTTGTCAGAACAATATGGGCGTCAGCCACGCCGGGTAAACCGat                                                              <  2:3621260/150‑1 (MQ=255)
                                                                                         ttCAGGGTCGCTTTCTGACTACCCAACCGGGAGTTCATGTCCATATTGGCGCCTCCGGTTAGGGTAACCTTATGCGGCTCTCCGCCACCATTTGGGCTGGTCTGGTTTGTAATAACAATTGGGGCGTCAGCCACGCCGGGTAAACCGata                                                             <  2:1342628/150‑1 (MQ=255)
                                                                                          tCATGGGCGCTTTCTGACTACCGAACAGGGTGTTCATGTCCAGTTTGGCGTCTCGGGTTTGGGTAACCTTATTCGGCTCTTCGCGACCAATTTGGCTGGTCAGGTTTGTCATAACAATTGGGGCGTCAGCCACGCCGGGTAAACCGatat                                                            <  1:3102731/150‑1 (MQ=255)
                                                                                              ggTCGCTTTCTGACTACCGAACAGGGAGTTCATGTCCAGATTGGCGTCTCCGGTTAGGGTAACCTTATTCGGCTCTTCGCGACCAATTTGGCTGGTCAGGTTTGTCAGAACAATATGGGCGTCAGCCACGCCGGGTAAACCGATATCttt                                                        <  2:3196968/150‑1 (MQ=255)
                                                                                              ggTCGCTTTCTGACTACCGAACAGGGAGTTCATGTCCAGATTGGCGTCTCCGGTTAGGGTAACCTTATTCGGCTCTTCGCGACCAATTTGGCTGGTCAGGTTTGTCAGAACAATATGGGCGTCAGCCACGCCGGGTAAACCGATATCttt                                                        <  2:2800726/150‑1 (MQ=255)
                                                                                               gTCGCTTTCTGACTACCGAACAGGGAGTTCATGTCCAGATTGGCGTCTCCGGTTAGGGTAACCTTATTCGGCTCTTCGCGACCAATTTGGCTGGTCAGGTTTGTCAGAACAATATGGGCGTCAGCCACGCCGGGTAAACCGATATCtttt                                                       >  2:4224703/1‑150 (MQ=255)
                                                                                               gTCGCTTTCTGACTACCGAACAGGGAGTTCATGTCCAGATTGGCGTCTCCGGTTAGGGTAACCTTATTCGGCTCTTCGCGACCAATTTGGCTGGTCAGGTTTGTCAGAACAATATGGGCGTCAGCCACGCCGGGTAAACCGATATCtttt                                                       >  2:4534886/1‑150 (MQ=255)
                                                                                                tCGCTTTCTGACTACCGAACTGGGAGTTCATGTCCAGATTGGCGTCTCCGGTTAGGGTAACCTTATTCGGCTCTTCGCGACCAATTTGGCTGGTCAGGTTTGTCATAACAATATGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTg                                                      <  1:3402973/150‑1 (MQ=255)
                                                                                                  gCTTTCTGACTACCGAACAGGTAGTTCATGTCCAGATTGGCGTCTCCGGTTAGGGTAACCTTATTCGGCTCTTCGCGACCAATTTGGCTGGTCAGGTTTGTCAGAACAATATGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTgag                                                    <  2:1904923/150‑1 (MQ=255)
                                                                                                  gCTTTCTGACTACCGAACAGGGAGTTCATGTCCAGATTGGCGTCTCCGGTTAGGGTAACCTTATTCGGCTCTTCGCGACCAATTTGGCTGGTCAGGTTTGTCAGAACAATTTGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTgag                                                    <  2:4340816/150‑1 (MQ=255)
                                                                                                  gCTTTCTGACTACCGAACAGGGAGTTCATGTCCAGATTGGCGTCTCCGGTTAGGGTAACCTTATTCGGCTCTTCGCGACCAATTTGGCTGGTCAGGTTTGTCAGAACAATATGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTgag                                                    <  2:3770697/150‑1 (MQ=255)
                                                                                                       cTGACTACCGAACAGGGAGTTCATGTCCAGATTGGCGTCTCCGGTTAGGGTAACCTTATTCGGCTCTTCGCTACAAATTTGGCTGGTCTGGTTTGTCAGAACAATATGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAatt                                               <  1:4711575/150‑1 (MQ=255)
                                                                                                       cTGACTACCGAACAGGGAGTTCATGTCCAGATTGGCGTCTCCGGTTAGGGTAACCTTATTCGGCTCTTCGCGACCAATTTGGCTGGTCAGGTTTGTCAGAACAATATGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAatt                                               >  1:3427767/1‑150 (MQ=255)
                                                                                                          aCTCCCTAACCGGGATTTCATGTCCAGTTTGGCGCCGCGGTTTTGGTTAACTTTTTTGGGCTGTTCGCGACAATTTGGGCTGTTCTGTTTTGTCATAAAATTTTGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAattatt                                            <  1:2341133/150‑1 (MQ=255)
                                                                                                            tACCGAACAGGGAGTTCATGTCCAGATTGGCGTCTCCGGTTAGGGTAACCTTATTCGGCTCTTCGCGACCAATTTGGCTGGTCAGGTTTGTCAGAACAATATGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATtattat                                          >  1:379400/1‑150 (MQ=255)
                                                                                                              ccGAACAGGGAGTTCTTGTCCAGATTGGCGTCTCCGGTTAGTGTAACCTTATTCGGCTCTTCGCGACCAATTTGGCTGGTCAGGTTTGTCAGAACAATATGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGt                                        <  2:3531468/150‑1 (MQ=255)
                                                                                                              ccGAACAGGGAGTTCATGTCCAGTTTGGCGTCTCCGGTTAGGGTAACCTTATTCGGCTCTTCGCGACCATTTTGGCTGGTCAGGTTTGTCAGAACAATATGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGt                                        <  2:4430526/150‑1 (MQ=255)
                                                                                                                 aaCAGGGAGTTCATGTCCAGATTGGCGTCTCCGGTTTGGGTAACCTTATTCGGCTCTTCGCGACCAATTTGGCTGGTCAGGTTTGTCAGAACAATATGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTc                                     <  2:1582303/150‑1 (MQ=255)
                                                                                                                 aaCAGGGAGTTCATGTCCAGATTGGCGTCTCCGGTTAGGGTAACCTTATTCGGCTCTGCGCGACCAATTTGGCTGGTCAGGTTTGTCAGAACAATATGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTc                                     <  2:4177492/150‑1 (MQ=255)
                                                                                                                 aaCAGGGAGTTCATGTCCAGATGGGCGTCTCCGGTTAGGGTAACCTTATTCGGCTCTTCGCGACCAATTTGGCTGGTCAGGTTTGTCAGAACAATATGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTc                                     <  2:1209719/150‑1 (MQ=255)
                                                                                                                       gAGTTCTTGTCCAGATTGGCGTCTCCGGTTTGGGTAACCTTATTCGGCTCTTCGCGACCATTTTGGCTGGTCAGGTTTGTCAGAACAATATGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGc                               <  2:1014743/150‑1 (MQ=255)
                                                                                                                        aGTTCATGTCCAGATTGGCGTCTCCGGTTAGGTTAACCTTATTCGGCTCTTCGCGACCAATTGGGCGGGTCTGGTTTGTCACAACAATATGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCg                              <  1:3992313/150‑1 (MQ=255)
                                                                                                                          ttCATGTCCAGATTGGCGTCTCCGGTTAGGGTAACCTTATTCGGCTCTTCGCGACCAATTTGGCTGGTCAGGTTTGTCAGAACAATATGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGAc                            <  2:4119439/150‑1 (MQ=255)
                                                                                                                           tCATGTCCTGATTGGCGTCTCCGGTTCGGGTAACCTTATTCGGCTCTTCGCGACCAATTGGGCTGGTCAGGTTTGTCAGAACAATTTGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACt                           <  2:4781725/150‑1 (MQ=255)
                                                                                                                             aTGTCCAGATTGGCTTCTCCGGTTTGGGTAACCTTATTCGGCTCTTCGCGACAATTTGGGCTGGTCAGGTTTGTCATAACAATATGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTgg                         <  1:3456354/150‑1 (MQ=255)
                                                                                                                              ggTCCCGATTGGCGTCTCCGTTTTGGGTAACTTTTTTCGGATCTACGCGACAAATTTGGCTGGTCTGGTTTGTCATAACATTTTGGGCGTCCGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTTTTATGTTTCGCAAGCGACTGGt                        <  1:1280583/149‑1 (MQ=255)
                                                                                                                               gTCCAGATTGGCGTCTCCGGTTAGGGTAACCTTATTCGGCTCTTCGCGACCAATTTGGCTGGTCAGGTTTGTCAGAACAATATGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGtt                       >  1:2540960/1‑150 (MQ=255)
                                                                                                                                  cAGATTGGCGTCTCCGGTTAGGGTAACCTTATTCGGCTCTTCGCGACCAATTTGGCTGGTCAGGTTTGTCAGAACAATATGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAAt                    >  1:2023251/1‑150 (MQ=255)
                                                                                                                                    gATTGGCGTCTCCGGTTAGGGTAACCTTATTCGGCTCTTCGCGACCAATTTGGCTGGTCAGGTTTGTCAGAACAATATGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAAttt                  >  1:2712447/1‑150 (MQ=255)
                                                                                                                                     aTTGGCGTCTCCGGTTTGGGTAACCTTATTGGGCTCTTCGCGACCATTTTGGCTGGTATGGTTTGTCAGAACAATATGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTc                 <  1:4271625/150‑1 (MQ=255)
                                                                                                                                     aTTGGCGTCTCCGGTTAGGGTAACCTTATTCGGCTCTTCGCGACCAATTTGGCTGGTCAGGTTTGTCAGAACAATATGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGACATTATTATGTTTCGCAAGCGACTGGTTAATTTc                 >  1:4047931/1‑150 (MQ=255)
                                                                                                                                     aTTGGCGTCTCCGGTTAGGGTAACCTTATTCGGCTCTTCGCGACCAATTTGGCTGGTCAGGTTTGTCAGAACAATATGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTc                 >  1:3618217/1‑150 (MQ=255)
                                                                                                                                           gTCTCCGGTTAGGGTAACCTTATTCGGCTCTTCGCGACCAATTTGGCTGGTCAGGTTTGTCAGAACAATATGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTc           <  2:4994587/150‑1 (MQ=255)
                                                                                                                                           gTCTCCGGTTAGGGTAACATTATTCGGCTCTTCGCGACCAATTTGGCTGGTCAGGTTTGTCAGAACAATATGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGATAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTc           >  2:5150875/1‑150 (MQ=255)
                                                                                                                                              tcCGGTTAGGTTAACCTTATTCGGCTCTTCGCGACCAATTTGGCTGGTCAGGTTTGTCAGAACAATATGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGt        <  2:2165750/150‑1 (MQ=255)
                                                                                                                                               cgggTTTGGTTAACCTTTTTCGGCTATTCGCCACAATTTTGGGGGTTCTTTTTTGACATAAAATTCGGGCCGACCCCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTg       <  1:1421519/148‑1 (MQ=255)
                                                                                                                                               ccGGTTTGGGTAACCTTTTTCGGCTCTTCGCGACCATTTTGGCTGGACTGTTTTGTCAGAACAATAGGGGCGCCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTg       <  1:5111600/150‑1 (MQ=255)
                                                                                                                                                 ggTTAGGGTAACCTTATTCGGCTCTTCGCGACCAATTTGGCTGGTCAGGTTTGTCAGAACAATATGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGAt     >  2:3575469/1‑150 (MQ=255)
                                                                                                                                                    tAGGGTAACCTTATTCGGCTCTTCGCGACCAATTTGGCTGGTCAGGTTTGTCAGAACAATATGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATGgt  >  1:2939210/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
GTTGTACCGTCGCATCGACCACTTCCATCTCTTTCAGGAAGATCGCACCTTTTTCTTTATCAAACACGGGCAGCGCTTTCAGCTTCAGTTTCATGGTCGCTTTCTGACTACCGAACAGGGAGTTCATGTCCAGATTGGCGTCTCCGGTTAGGGTAACCTTATTCGGCTCTTCGCGACCAATTTGGCTGGTCAGGTTTGTCAGAACAATATGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATGGT  >  CP009273/1118357‑1118654

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑