BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	1,308,862	A→C	10.2%	S196R (AGC→CGC)	ompW →	outer membrane protein W

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	1,308,862	0	A	C	10.2%	492.4 / 2.8	196	S196R (AGC→CGC)	ompW	outer membrane protein W
Reads supporting (aligned to +/- strand): ref base A (98/70); new base C (2/25); total (100/96)
Fisher's exact test for biased strand distribution p-value = 3.81e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 199 total aligned reads displayed.

TCTCAGTCTGAAAGATTCCTGGGGAGCTGCCGGGCAGGTGGGGGTTGATTATCTGATTAACCGTGACTGGTTGGTTAACATGTCAGTGTGGTACATGGATATCGATACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGA  >  CP009273/1308714‑1309011

tctcAGTCTGAAAGATTCCTGGGGAGCTGCCGGGCAGGTGGGGGTTGATTATCTGATTAACCGTGACTGGTTGGTTAACATGTCAGTGTGGTACATGGATATCGATACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAg                                                                                                                                                      >  1:3027534/1‑150 (MQ=255)

   cAGTCTGAAAGATTCCTGGGGAGCTGCCGGGCAGGTGGGGGTTGATTATCTGATTAACCGTGACTGGTTGGTTAACATGTCAGTGTGGTACATGGATATCGATACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGt                                                                                                                                                   <  2:1644837/150‑1 (MQ=255)

    aGTCTGAAAGATTCCTGGGGAGCTGCCGGGCAGGTGGGGGTTGATTATCTGATTAACCGTGACTGGTTGGTTAACATGTCAGTGTGGTACATGGATATCGATACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTa                                                                                                                                                  <  2:3645500/150‑1 (MQ=255)

    aGTCTGAAAGATTCCTGGGGAGCTGCCGGGCAGGTGGGGGTTGATTATCTGATTAACCGTGACTGGTTGGTTAACATGTCAGTGTGGTACATGGATATCGATACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTa                                                                                                                                                  >  1:3789224/1‑150 (MQ=255)

      tCTGAAAGATTCCTGGGGAGCTGCCGGGCAGGTGGGGGTTGATTATCTGATTAACCGTGACTGGTTGGTTAACATGTCAGTGTGGTACATGGATATCGATACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACg                                                                                                                                                <  1:4216252/150‑1 (MQ=255)

         gAAAGAGTCCTGGGGAGCTGCCGGGCAGGTGGGGGTTGATTATCTGATTAACCGTGACTGGTTGGTTAACATGTCAGTGTGGTACATGGATATCGATACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCt                                                                                                                                             <  2:4507147/150‑1 (MQ=255)

          aaaGATTCCTGGGGAGCTGCCGGGCAGGTGGGGGTTGATTATCTGATTAACCGTGACTGGTTGGTTAACATGTCAGTGTGGTACATGGATATCGATACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTc                                                                                                                                            <  1:773148/150‑1 (MQ=255)

          aaaGATTCCTGGGGAGCTGCCGGGCAGGTGGGGGTTGATTATCTGATTAACCGTGACTGGTTGGTTAACATGTCAGTGTGGTACATGGATATCGATACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTc                                                                                                                                            <  2:4523735/150‑1 (MQ=255)

            aGATTCCTGGGGAGCTGCCGGGCAGGTGGGGGTTGATTATCTGATTAACCGTGACTGGTTGGTTAACATGTCAGTGTGGTACATGGATATCGATACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGa                                                                                                                                          >  2:1022730/1‑150 (MQ=255)

              aTTCCTGGGGAGCTGCCGGGCAGGTGGGGGTTGATTATCTGATTAACCGTGACTGGTTGGTTAACATGTCAGTGTGGTACATGGATATCGATACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATc                                                                                                                                        >  1:2305622/1‑150 (MQ=255)

                 ccTGGGGAGCTGCCGGGCAGGTGGGGGTTGATTATCTGATTAACCGTGACTGGTTGGTTAACATGTCAGTGTGGTACATGGATATCGATACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGt                                                                                                                                     >  1:409897/1‑150 (MQ=255)

                    ggggAGCTGCCGGGCAGGTGGGGGTTGATTATCTGATTAACCGTGACTGGTTGGTTAACATGTCAGTGTGGTACATGGATATCGATACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTggg                                                                                                                                  >  2:901965/1‑150 (MQ=255)

                     gggAGCTGCCGGGCAGGTGGGGGTTGATTATCTGATTAACCGTGACTGGTTGGTTAACATGTCAGTGTGGTACATGGATATCGATACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGgt                                                                                                                                 >  2:224820/1‑150 (MQ=255)

                      ggAGCTGCCGGGCAGGTGGGGGTTGATTATCTGATTAACCGTGACTGGTTGGTTAACATGTCAGTGTGGTACATGGATATCGATACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGgtg                                                                                                                                >  2:2339390/1‑150 (MQ=255)

                       gAGCTGCCGGGCAGGTGGGGGTTGATTATCTGATTAACCGTGACTGGTTGGTTAACATGTCAGTGTGGTACATGGATATCGATACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGgtgt                                                                                                                               <  2:135647/150‑1 (MQ=255)

                        aGCTGCCGGGCAGGTGGGGGTTGATTATCTGATTAACCGTGACTGGTTGGTTAACATGTCAGTGTGGTACATGGATATCGATACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGtt                                                                                                                              <  1:2660058/150‑1 (MQ=255)

                         gCTGCCGGGCAGGTGGGGGTTGATTATCTGATTAACCGTGACTGGTTGGTTAACATGTCAGTGTGGTACATGGATATCGATACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGttt                                                                                                                             <  2:4434946/150‑1 (MQ=255)

                           tGCCGGGCAGGTGGGGGTTGATTATCTGATTAACCGTGACTGGTTGGTTAACATGTCAGTGTGGTACATGGATATCGATACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTAt                                                                                                                           <  1:4450519/150‑1 (MQ=255)

                              cGGGCAGGTGGGGGTTGATTATCTGATTAACCGTGACTGGTTGGTTAACATGTCAGTGTGGTACATGGATATCGATACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATCGCGTACGCCTCGATCCGTGGGTGTTTATGtt                                                                                                                        >  2:5174002/1‑150 (MQ=255)

                              cGGGCAGGTGGGGGTTGATTATCTGATTAACCGTGACTGGTTGGTTAACATGTCAGTGTGGTACATGGATATCGATACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGtt                                                                                                                        <  1:510218/150‑1 (MQ=255)

                              cGGGCAGGTGGGGGTTGATTATCTGATTAACCGTGACTGGTTGGTTAACATGTCAGTGTGGTACATGGATATCGATACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGtt                                                                                                                        >  2:4610588/1‑150 (MQ=255)

                              cGGGCAGGTGGGGGTTGATTATCTGATTAACCGTGACTGGTTGGTTAACATGTCAGTGTGGTACATGGATATCGATACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGGGGGTGTTTATGtt                                                                                                                        >  2:4610453/1‑150 (MQ=255)

                               gggCAGGTGGGGGTTGATTATCTGATTAACCGTGACTGGTTGGTTAACATGTCAGTGTGGTACATGGATATCGATACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTtc                                                                                                                       >  2:3838470/1‑150 (MQ=255)

                               gggCAGGTGGGGGTTGATTATCTGATTAACCGTGACTGGTTGGTTAACATGTCAGTGTGGTACATGGATATCGATACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTtc                                                                                                                       <  2:150402/150‑1 (MQ=255)

                                  cAGGTGGGGGTTGATTATCTGATTAACCGTGACTGGTTGGTTAACATGTCAGTGTGGTACATGGATATCGATACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTca                                                                                                                    <  1:4502177/150‑1 (MQ=255)

                                      tGGGGGTTGATTATCTGATTAACCGTGACTGGTTGGTTAACATGTCAGTGTGGTACATGGATATCGATACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTcagcag                                                                                                                >  1:3577244/1‑150 (MQ=255)

                                       gggggTTGATTATCTGATTAACCGTGACTGGTTGGTTAACATGTCAGTGTGGTACATGGATATCGATACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAgg                                                                                                               >  2:4646641/1‑150 (MQ=255)

                                       gggggTTGATTATCTGATTAACCGTGACTGGTTGGTTAACATGTCAGTGTGGTACATGGATATCGATACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAgg                                                                                                               >  2:2086566/1‑150 (MQ=255)

                                           gTTGATTATCTGATTAACCGTGACTGGTTGGTTAACATGTCAGTGTGGTACATGGATATCGATACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGatat                                                                                                           <  1:524146/150‑1 (MQ=255)

                                            ttGATTATCTGATTAACCGTGACTGGTTGGTTAACATGTCAGTGTGGTACATGGATATCGATACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATc                                                                                                          >  2:4693852/1‑150 (MQ=255)

                                               aTTATCTGATTAACCGTGACTGGTTGGTTAACATGTCAGTGTGGTACATGGATATCGATACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGtt                                                                                                       <  2:774974/150‑1 (MQ=255)

                                               aTTATCTGATTAACCGTGACTGGTTGGTTAACATGTCAGTGTGGTACATGGATATCGATACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGtt                                                                                                       <  1:196343/150‑1 (MQ=255)

                                                  aTCTGATTAACCGTGACTGGTTGGTTAACATGTCAGTGTGGTACATGGATATCGATACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCATACGCCTCGATCCGGGGGTGTTTATGTTCTCAGCAGGATATCGttttt                                                                                                    >  2:2606745/1‑150 (MQ=255)

                                                    cTGATTAACCGTGACTGGTTGGTTAACATGTCAGTGTGGTACATGGATATCGATACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTaa                                                                                                  <  2:3921376/150‑1 (MQ=255)

                                                     tGATTAACCGTGACTGGTTGGTTAACATGTCAGTGTGGTACATGGATATCGATACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAAt                                                                                                 >  2:4318822/1‑150 (MQ=255)

                                                         tAACCGTGACTGGTTGGTTAACATGTCAGTGTGGTACATGGATATCGATACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCg                                                                                             >  2:1771641/1‑150 (MQ=255)

                                                         tAACCGTGACTGGTTGGTTAACATGTCAGTGTGGTACATGGATATCGATACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGCCTATCGTTTTTAATTCCg                                                                                             >  1:3076040/1‑150 (MQ=255)

                                                              gTGACTGGTTGGTTAACATGTCAGTGTGGTACATGGATATCGATACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGcacac                                                                                        >  1:2381213/1‑149 (MQ=255)

                                                              gTGACTGGTTGGTTAACATGTCAGTGTGGTACATGGATATCGATACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACaa                                                                                        >  1:584344/1‑150 (MQ=255)

                                                              gTGACTGGTTGGTTAACATGTCAGTGTGGTACATGGATATCGATACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACaa                                                                                        >  1:3191470/1‑150 (MQ=255)

                                                                 aCTGGTTGGTTAACATGTCAGTGTGGTACATGGATATCGATACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCCCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACaaaaa                                                                                     <  1:3409404/150‑1 (MQ=255)

                                                                  cTGGTTGGTTAACATGTCAGTGTGGTACATGGATATCGATACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAAc                                                                                    >  1:1888639/1‑150 (MQ=255)

                                                                     gttggttAACATGTCAGTGTGGTACATGGATATCGATACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGAc                                                                                 >  1:1777852/1‑150 (MQ=255)

                                                                           tAACATGTCAGTGTGGTACATGGATATCGATACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTa                                                                           >  2:4089590/1‑150 (MQ=255)

                                                                              cttGTCTGTGTGGTACATGGATATCGATACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGTTCGCGTACGCCTCGATCCGTGGGTTTTTATGTTATCAGCTGGATATCTTTTTTAATTCCGCACAAAAACGACCCCGTAata                                                                        <  1:4320560/148‑1 (MQ=255)

                                                                               aTGTCAGTGTGGTACATGGATATCGATACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAatat                                                                       >  2:1061515/1‑150 (MQ=255)

                                                                                tGTCTGTGTGGTACATGGATATCGATACCACCGCAATTTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGTATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAAtata                                                                      <  2:4928236/150‑1 (MQ=255)

                                                                                    tgtgtgtTACTTGTATATCTATACCACCGCAAATTATATGGTGGGGGGTGCACAGCAACACGATAGCGTACGCCTCGAGCCGTGGGTTTTTTTGTTCTCCGCAGTATTTCTTTTTTATTTCCGCACAAACACGACCCCGTAATATACggg                                                                  <  1:3180225/149‑1 (MQ=255)

                                                                                     gtgtGGTACATGGATATCGATACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACgggg                                                                 >  2:3608304/1‑150 (MQ=255)

                                                                                       gtgGTACATGGATATCGATACCACCGCCAATTATAAGCTGGGCGGTGCACACCAACACGATCGCGTACGCCTCGATCCGTGGGTTTTTATGTTCTCAGCAGGAAATCTTTTTTAATTCCGCACAAACACGACCCCGTAATATACGGGGTc                                                               <  2:2659775/150‑1 (MQ=255)

                                                                                        tgGTACATGGATATCGATACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCa                                                              >  1:3456700/1‑150 (MQ=255)

                                                                                         gttACATGTATATCGATACCACCGCCAATTATAAGCTGGGCGGTGCACAGAAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTTTGTTATCAGCAGGATATCTTTTTTAATTCCGCACAAACACGACCCCGTAATATACGGGGTCaa                                                             <  1:2939405/148‑1 (MQ=255)

                                                                                         ggTACATGGATATCGATACCCCCCCCAATTATACGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCaa                                                             <  2:1256178/150‑1 (MQ=255)

                                                                                            aCATGGATATCGCTACCACCGCCAATTATAAGCTGGGCGGGGCACAGCAACCCGAGAGCGTACGCCTCGATCCGTGGGTGTTTATGTGCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATaa                                                          <  2:360931/150‑1 (MQ=255)

                                                                                            aCATGGATATCGATACCACCGCCAATTATAAGCTGGGCGGTGCACAGAAACACGATCGCGTACGCCTCGATCCGTGGGTTTTTATGTTCTCAGCAGTATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATaa                                                          <  1:4668841/150‑1 (MQ=255)

                                                                                             cATGGATATCGATACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCaagaag                                                         >  2:474444/1‑150 (MQ=255)

                                                                                             cATGGATATCGATACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAg                                                         >  2:3594707/1‑150 (MQ=255)

                                                                                             cATGGATATCGATACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAg                                                         >  2:3195423/1‑150 (MQ=255)

                                                                                              ttGGATATCGATACCACCGCAATTTTTATGCTGGGGGGTGCACAGAAACACTTTAGCGTACGCCTCGATCCGTGGGTGTTTTTGTTCTCAGCTGGATTTCTTTTTTAATTCCGCAAAAAAACGACCCCGTAATATACGGGGTCAATAAgg                                                        <  1:4068106/149‑1 (MQ=255)

                                                                                              agggATATCGATCCCACCCCCAATTATATGCTGGGCGGTGCACCGCAACCCTATTGCGTACGCATCGATCGGGGGGTTTTTATGTACACCGCAGGATATCTTTTTTATTTCCGCAAAAAAACGACCCCGTAATATACGGGGTCAATAAgg                                                        <  1:1542833/148‑1 (MQ=255)

                                                                                                 gATATCGATACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACa                                                     >  1:3877736/1‑150 (MQ=255)

                                                                                                   tatCGATACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATCTCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATg                                                   >  1:4887031/1‑150 (MQ=255)

                                                                                                    atCGATACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATgg                                                  >  1:1437190/1‑150 (MQ=255)

                                                                                                       gagaccaccGCCAATTCTAAGCTGGGCGGTGCACAGCAACCCGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGtat                                               <  2:288582/147‑1 (MQ=255)

                                                                                                       gATACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGtat                                               >  1:3422721/1‑150 (MQ=255)

                                                                                                        atcccaccGCCAATTATACGCGGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGACCCGGGGGTGTTTATGTTCTCAGCCGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGtata                                              <  1:3037688/147‑1 (MQ=255)

                                                                                                        aTACCACCGCCAATTATACGCTGGGCGGTGCACAGCAACCCGACCGCGAACGCCCCGACCTGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGAACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGtata                                              <  2:1326566/150‑1 (MQ=255)

                                                                                                        aTACCACCGCCAATTATAAGCTGGGCGGGGCACAGAAACACGATAGCGTCCGCCTCGATCCGTGGGTGTTTATGTTCTCACCCGTATATCTTTTTTAATTCCGCAAAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGtata                                              <  1:1178525/150‑1 (MQ=255)

                                                                                                         tcccaccGCCAATTCTAAGCGGGGCGGTGCACCGCACCACGAGAGCGTACGCCTCGATCCGTGGGTGTTTATGTGCTCAGCCGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATaa                                             <  2:3288802/148‑1 (MQ=255)

                                                                                                           ccaccGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAg                                           >  1:4853000/1‑150 (MQ=255)

                                                                                                           ccaccGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAg                                           >  1:257078/1‑150 (MQ=255)

                                                                                                             accGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAggg                                         >  2:3785606/1‑150 (MQ=255)

                                                                                                                 ccAATTATACGCGGGGCGGTGCACAGCAACACGATCGCTTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATGGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGtatt                                     <  2:3626858/150‑1 (MQ=255)

                                                                                                                 ccAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAGAACGACCCCGTAATCTACGGGGTCAAGAAGGACATGGTATCAAGGGtatt                                     >  1:2020741/1‑150 (MQ=255)

                                                                                                                 ccAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGtatt                                     <  2:3627400/150‑1 (MQ=255)

                                                                                                                  cAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGtatta                                    >  1:3554065/1‑150 (MQ=255)

                                                                                                                  cAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGtatta                                    <  2:4264827/150‑1 (MQ=255)

                                                                                                                   aaTTATAAGGTGGGCGGTGCACACCAACACGATAGCGTCCGCCTCGATCCGTGGGTTTTTTTGTTCTCAGCAGGATATCTTTTTTATTTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGtattat                                   <  1:1724083/150‑1 (MQ=255)

                                                                                                                   aaTTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGtattat                                   <  2:4335363/150‑1 (MQ=255)

                                                                                                                      tataAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATttc                                >  2:2702019/1‑150 (MQ=255)

                                                                                                                       ataAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATttct                               >  2:1092140/1‑150 (MQ=255)

                                                                                                                         acgCTGGGGGGTGCACAGCAACACGATCGCGTACGCCTCGAGCCGTGGGTGTTTATGTTCTCAGCATGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATttcttc                             <  2:4358069/148‑1 (MQ=255)

                                                                                                                         acgCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATttcttc                             <  1:2994805/148‑1 (MQ=255)

                                                                                                                         aaGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATttcttc                             >  2:2363379/1‑150 (MQ=255)

                                                                                                                             tGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCtt                         >  2:2022498/1‑150 (MQ=255)

                                                                                                                               ggCGGTGCACAGCAACACGCTCGCGTACGCCTCGATCGGTGGGTGTTTTTGTTCTCAGCCGGATATCTTTTTTAATGCCGCACAAACACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCt                       <  1:3319630/150‑1 (MQ=255)

                                                                                                                                    tGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGcc                  >  2:1208659/1‑150 (MQ=255)

                                                                                                                                      cacaGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCAt                >  1:4827238/1‑150 (MQ=255)

                                                                                                                                        caGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGATTCTACGCCATCa              >  1:2230359/1‑150 (MQ=255)

                                                                                                                                         aGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCcagcag             >  2:3983640/1‑150 (MQ=255)

                                                                                                                                         aGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAg             >  1:334055/1‑150 (MQ=255)

                                                                                                                                           cAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATAGCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATCCGGGGTCACTACGGAGATGGTATCAAGGGTATTATTTCTTCGCTTCTAAGCCATCAggt           >  2:4517076/1‑148 (MQ=255)

                                                                                                                                            aaCCCGATCGCGTACGCCTCGATCCTTGGTTGTTTTTGTACTCAGCAGTATATCGTTTTTAATTCCGCACAAACACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGttt          <  1:1610143/150‑1 (MQ=255)

                                                                                                                                              cccGATAGCGTACGCCTCGATCCGTGGGTGTTAATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCa        <  2:4292487/148‑1 (MQ=255)

                                                                                                                                              cacGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCa        <  2:5038168/150‑1 (MQ=255)

                                                                                                                                              cacGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCa        >  2:1984636/1‑150 (MQ=255)

                                                                                                                                               acGATCGCGTCCGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCTTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCag       <  2:2380332/150‑1 (MQ=255)

                                                                                                                                                ctaTAGCTTACCCCTCGATCCGTGGGTGTTTTTTTTCTCAGCTTTATTTGTTTTTTAATCCCGAAAAAACACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCaga      <  1:2086566/148‑1 (MQ=255)

                                                                                                                                                 gATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCagag     <  2:3394157/150‑1 (MQ=255)

                                                                                                                                                    aGCTTACGCCTCGATCGGTGGGTTTTTATGTTCTCTGCAGTATATCTTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGa  <  1:4982642/150‑1 (MQ=255)

TCTCAGTCTGAAAGATTCCTGGGGAGCTGCCGGGCAGGTGGGGGTTGATTATCTGATTAACCGTGACTGGTTGGTTAACATGTCAGTGTGGTACATGGATATCGATACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGA  >  CP009273/1308714‑1309011

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`