BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	1,472,138	G→T	20.1%	A87A (GCG→GCT)	ynbA →	inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	1,472,138	0	G	T	20.1%	614.3 / 119.9	228	A87A (GCG→GCT)	ynbA	inner membrane protein
Reads supporting (aligned to +/- strand): ref base G (98/84); new base T (23/23); total (121/107)
Fisher's exact test for biased strand distribution p-value = 7.41e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 229 total aligned reads displayed.

CTTTCTCTTCTTACCGGATTGCTGTTGATGTTGGCGGCACAACCCATCCTCTTTTTGCTATTGCCCATCGTGCTTTTTATCCGTATGGCGCTCAATGCGCTGGATGGCATGTTGGCGCGTGAGTGCAACCAGCAAACACGTCTGGGGGCGATTTTGAATGAGACTGGCGATGTTATTTCCGATATTGCGCTCTATTTACCATTTTTATTTTTACCGGAAAGTAACGCATCACTCGTGATACTCATGTTATTTTGCACCATATTGACCGAGTTTTGCGGTTTACTCGCGCAGACGATTA  >  CP009273/1471989‑1472286

cTTTCTCTTCTTACCGGATTGCTGTTGATGTTGGCGGCACAACCCATCCTCTTTTTGCTATTGCCCATCGTGCTTTTTATCCGTATGGCGCTCAATGCGCTGGATGGCATGTTGGCGCGTGAGTGCAACCAGCAAACACGTCTGGGGGCg                                                                                                                                                      >  2:4319531/1‑150 (MQ=255)

 tttCTCTTCTTACCGGATTGCTGTTGATGTTGGCGGCACAACCCATCCTCTTTTTGCTATTGCCCATCGTGCTTTTTATCCGTATGGCGCTCAATGCGCTGGATGGCATGTTGGCGCGTGAGTGCAACCAGCAAACACGTCTGGGGGCGa                                                                                                                                                     >  2:3579440/1‑150 (MQ=255)

 tttCTCTTCTTACCGGATTGCTGTTGATGTTGGCGGCACAACCCATCCTCTTTTTGCTATTGCCCATCGTGCTTTTTATCCGTATGGCGCTCAATGCGCTGGATGGCATGTTGGCGCGTGAGTGCAACCAGCAAACACGTCTGGGGGCGa                                                                                                                                                     >  2:3296623/1‑150 (MQ=255)

 tttCTCTTCTTACCGGATTGCTGTTGATGTTGGCGGCACAACCCATCCTCTTTTTGCTATTGCCCATCGTGCTTTTTATCCGTATGGCGCTCAATGCGCTGGATGGCATGTTGGCGCGTGAGTGCAACCAGCAAACACGTCTGGGGGCGa                                                                                                                                                     >  2:3793852/1‑150 (MQ=255)

  ttCTCTTCTTACCGGATTGCTGTTGATGTTGGCGGCACAACCCATCCTCTTTTTGCTATTGCCCATCGTGCTTTTTATCCGTATGGCGCTCAATGCGCTGGATGGCATGTTGGCGCGTGAGTGCAACCAGCAAACACGTCTGGGGGCGAt                                                                                                                                                    >  1:3915444/1‑150 (MQ=255)

    ctctTCTTACCGGATTGCTGTTGATGTTGGCGGCACAACCCATCCTCTTTTTGCTATTGCCCATCGTGCTTTTTATCCGTATGGCGCTCAATGCGCTGGATGGCATGTTGGCGCGTGAGTGCAACCAGCAAACACGTCTGGGGGCTAttt                                                                                                                                                  <  2:5030080/150‑1 (MQ=255)

    ctctTCTTACCGGATTGCTGTTGATGTTGGCGGCACAACCCATCCTCTTTTTGCTATTGCCCATCGTGCTTTTTATCCGTATGGCGCTCAATGCGCTGGATGGCATGTTGGCGCGTGAGTGCAACCAGCAAACACGTCTGGGGGCTAttt                                                                                                                                                  <  2:855892/150‑1 (MQ=255)

    ctctTCTTACCGGATTGCTGTTGATGTTGGCGGCACAACCCATCCTCTTTTTGCTATTGCCCATCGTGCTTTTTATCCGTATGGCGCTCAATGCGCTGGATGGCATGTTGGCGCGTGAGTGCAACCAGCAAACACGTCTGGGGGCGAttt                                                                                                                                                  >  2:1793473/1‑150 (MQ=255)

     tcttctTACCGGATTGCTGTTGATGTTGGCGGCACAACCCATCCTCTTTTTGCTATTGCCCATCGTGCTTTTTATCCGTATGGCGCTCAATGCGCTGGATGGCATGTTGGCGCGTGAGTGCAACCAGCAAACACGTCTGGGGGCGAtttt                                                                                                                                                 <  1:3482006/150‑1 (MQ=255)

     tcttctTACCGGATTGCTGTTGATGTTGGCGGCACAACCCATCCTCTTTTTGCTATTGCCCATCGTGCTTTTTATCCGTATGGCGCTCAATGCGCTGGATGGCATGTTGGCGCGTGAGTGCAACCAGCAAACACGTCTGGGGGCGAtttt                                                                                                                                                 <  2:2185119/150‑1 (MQ=255)

      cttcttACCGGATTGCTGTTGATGTTGGCGGCACAACCCATCCTCTTTTTGCTATTGCCCATCGTGCTTTTTATCCGTATGGCGCTCAATGCGCTGGATGGCATGTTGGCGCGTGAGTGCAACCAGCAAACACGTCTGGGGGCGATTTTg                                                                                                                                                <  1:1046403/150‑1 (MQ=255)

          ttACCGGATTGCTGTTGATGTTGGCGGCACAACCCATCCTCTTTTTGCTATTGCCCATCGTGCTTTTTATCCGTATGGCGCTCAATGCGCTGGATGGCATGTTGGCGCGTGAGTGCAACCAGCAAACACGTCTGGGGGCGATTTTGAATg                                                                                                                                            >  1:2719597/1‑150 (MQ=255)

           tACCGGATTGCTGTTGATGTTGGCGGCACAACCCATCCTCTTTTTGCTATTGCCCATCGTGCTTTTTATCCGTATGGCGCTCAATGCGCTGGATGGCATGTTGGCGCGTGAGTGCAACCAGCAAACACGTCTGGGGGCGATTTTGAATga                                                                                                                                           >  2:2371849/1‑150 (MQ=255)

              cGGATTGCTGTTGATGTTGGCGGCACAACCCATCCTCTTTTTGCTATTGCCCATCGTGCTTTTTATCCGTATGGCGCTCAATGCGCTGGATGGCATGTTGGCGCGTGAGTGCAACCAGCAAACACGTCTGGGGGCGATTTTGAATGAGAc                                                                                                                                        <  1:4686152/150‑1 (MQ=255)

               ggATTGCTGTTGATGTTGGCGTCACAACCCATCCTCTTTTTGCTATTGCCCATCGTGCTTTTTATCCGTATGGCGCTCAATGCGCTGGATGGCATGTTGGCGCGTGAGTGCAACCAGCAAACACGTCTGGGGGCGATTTTGAATGAGACt                                                                                                                                       >  1:1780155/1‑150 (MQ=255)

                  ttGCTGTTGATGTTGGCGGCACAACCCATCCTCTTTTTGCTATTGCCCATCGTGCTTTTTATCCGTATGGCGCTCAATGCGCTGGATGGCATGTTGGCGCGTGAGTGCAACCAGCAAACACGTCTGGGGGCGATTTTGAATGAGACTGGc                                                                                                                                    >  2:4822070/1‑150 (MQ=255)

                  ttGCTGTTGATGTTGGCGGCACAACCCATCCTCTTTTTGCTATTGCCCATCGTGCTTTTTATCCGTATGGCGCTCAATGCGCTGGATGGCATGTTGGCGCGTGAGTGCAACCAGCAAACACGTCTGGGGGCGATTTTGAATGAGACTGGc                                                                                                                                    <  2:5092402/150‑1 (MQ=255)

                   tGCTGTTGATGTTGGCGGCACAACCCATCCTCTTTTTGCTATTGCCCATCGTGCTTTTTATCCGTATGGCGCTCAATGCGCTGGATGGCATGTTGGCGCGTGAGTGCAACCAGCAAACACGTCTGGGGGCGATTTTGAATGAGACTGGCg                                                                                                                                   <  2:4789299/150‑1 (MQ=255)

                      tGTTGATGTTGGCGGCACAACCCATCCTCTTTTTGCTATTGCCCATCGTGCTTTTTATCCGTATGGCGCTCAATGCGCTGGATGGCATGTTGGCGCGTGAGTGCAACCAGCAAACACGTCTGGGGGCGATTTTGAATGAGACTGGCGATg                                                                                                                                <  2:2849268/150‑1 (MQ=255)

                      tGTTGATGTTGGCGGCACAACCCATCCTCTTTTTGCTATTGCCCATCGTGCTTTTTATCCGTATGGCGCTCAATGCGCTGGATGGCATGTTGGCGCGTGAGTGCAACCAGCAAACACGTCTGGGGGCGATTTTGAATGAGACTGGCGATg                                                                                                                                >  2:652882/1‑150 (MQ=255)

                         tGATGTTGGCGGCACAACCCATCCTCTTTTTGCTATTGCCCATCGTGCTTTTTATCCGTATGGCGCTCAATGCGCTGGATGGCATGTTGGCGCGTGAGTGCAACCAGCAAACACGTCTGGGGGCGATTTTGAATGAGACTGGCGATGTTa                                                                                                                             <  1:1361683/150‑1 (MQ=255)

                           aTGTTGGCGGCACAACCCATCCTCTTTTTGCTATTGCCCATCGTGCTTTTTATCCGTATGGCGCTCAATGCGCTGGATGGCATGTTGGCGCGTGAGTGCAACCAGCAAACACGTCTGGGGGCGATTTTGAATGAGACTGGCGATGTTAtt                                                                                                                           <  2:4457382/150‑1 (MQ=255)

                             gTTGGCGGCACAACCCATCCTCTTTTTGCTATTGCCCATCGTGCTTTTTATCCGTATGGCGCTCAATGCGCTGGATGGCATGTTGGCGCGTGAGTGCAACCAGCAAACACGTCTGGGGGCGATTTTGAATGAGACTGGCGATGTTATTTc                                                                                                                         <  2:3548588/150‑1 (MQ=255)

                                ggcggcACAACCCATCCTCTTTTTGCTATTGCCCATCGTGCTTTTTATCCGTATGGCGCTCAATGCGCTGGATGGCATGTTGGCGCGTGAGTGCAACCAGCAAACACGTCTGGGGGCTATTTTGAATGAGACTGGCGATGTTATTTCCGa                                                                                                                      <  2:1765116/150‑1 (MQ=255)

                                  cggcACAACCCATCCTCTTTTTGCTATTGCCCATCGTGCTTTTTATCCGTATGGCGCTCAATGCGCTGGATGGCATGTTGGCGCGTGAGTGCAACCAGCAAACACGTCTGGGGGCGATTTTGAATGAGACTGGCGATGTTATTTCCGata                                                                                                                    >  2:3459446/1‑150 (MQ=255)

                                   ggcACAACCCATCCTCTTTTTGCTATTGCCCATCGTGCTTTTTATCCGTATGGCGCTCAATGCGCTGGATGGCATGTTGGCGCGTGAGTGCAACCAGCAAACACGTCTGGGGGCGATTTTGAATGAGACTGGCGATGTTATTTCCGatat                                                                                                                   >  2:729530/1‑150 (MQ=255)

                                   ggcACAACCCATCCTCTTTTTGCTATTGCCCATCGTGCTTTTTATCCGTATGGCGCTCAATGCGCTGGATGGCATGTTGGCGCGTGAGTGCAACCAGCAAACACGTCTGGGGGCGATTTTGAATGAGACTGGCGATGTTATTTCCGatat                                                                                                                   >  1:2564539/1‑150 (MQ=255)

                                   ggcACAACCCATCCTCTGTTTGCTATTGCCCATCGTGCTTTTTATCCGTATGGCGCTCAATGCGCTGGATGGCATGTTGGCGCGTGAGTGCAACCAGCAAACACGTCTGGGGGCGATTTTGAATGAGACTGGCGATGTTATTTCCGatat                                                                                                                   <  1:3592618/150‑1 (MQ=255)

                                    gcACAACCCATCCTCTTTTTGCTATTGCCCATCGTGCTTTTTATCCGTATGGCGCTCAATGCGCTGGATGGCATGTTGGCGCGTGAGTGCAACCAGCAAACACGTCTGGGGGCTATTTTGAATGAGACTGGCGATGTTATTTCCGATAtt                                                                                                                  <  1:1698478/150‑1 (MQ=255)

                                     cacaACCCATCCTCTTTTTGCTATTGCCCATCGTGCTTTTTATCCGTATGGCGCTCAATGCGCTGGATGGCATGTTGGCGCGTGAGTGCAACCAGCAAACACGTCTGGGGGCTATTTTGAATGAGACTGGCGATGTTATTTCCGATATTg                                                                                                                 >  2:3115119/1‑150 (MQ=255)

                                     cacaACCCATCCTCTTTTTGCTATTGCCCATCGTGCTTTTTATCCGTATGGCGCTCAATGCGCTGGATGGCATGTTGGCGCGTGAGTGCAACCAGCAAACACGTCTGGGGGCTATTTTGAATGAGACTGGCGATGTTATTTCCGATATTg                                                                                                                 >  2:3115006/1‑150 (MQ=255)

                                     cacaACCCATCCTCTTTTTGCTATTGCCCATCGTGCTTTTTATCCGTATGGCGCTCAATGCGCTGGATGGCATGTTGGCGCGTGAGTGCAACCAGCAAACACGTCTGGGGGCGATTTTGAATGAGACTGGCGATGTTATTTCCGATATTg                                                                                                                 >  1:472208/1‑150 (MQ=255)

                                     cacaACCCATCCTCTTTTTGCTATTGCCCATCGTGCTTTTTATCCGTATGGCGCTCAATGCGCTGGATGGCATGTTGGCGCGTGAGTGCAACCAGCAAACACGTCTGGGGGCGATTTTGAATGAGACTGGCGATGTTATTTCCGATATTg                                                                                                                 >  1:3080259/1‑150 (MQ=255)

                                       caACCCATCCTCTTTTTGCTATTGCCCATCGTGCTTTTTATCCGTATGGCGCTCAATGCGCTGGATGGCATGTTGGCGCGTGAGTGCAACCAGCAAACACGTCTGGGGGCTATTTTGAATGAGACTGGCGATGTTATTTCCGATATTgcg                                                                                                               >  2:1879152/1‑150 (MQ=255)

                                         aCCCATCCTCTTTTTGCTATTGCCCATCGTGCTTTTTATCCGTATGGCGCTCAATGCGCTGGATGGCATGTTGGCGCGTGAGTGCAACCAGCAAACACGTCTGGGGGCTATTTTGAATGAGACTGGCGATGTTATTTCCGATATTGCGct                                                                                                             <  2:3897933/150‑1 (MQ=255)

                                            cATCCTCTTTTTGCTATTGCCCATCGTGCTTTTTATCCGTATGGCGCTCAATGCGCTGGATGGCATGTTGGCGCGTGAGTGCAACCAGCAAACACGTCTGGGGGCGATTTTGAATGAGACTGGCGATGTTATTTCCGATATTGCGCTCTa                                                                                                          <  1:2250991/150‑1 (MQ=255)

                                                ctctTTTTGCTATTGCCCATCGTGCTTTTTATCCGTATGGCGCTCAATGCGCTGGATGGCATGTTGGCGCGTGAGTGCAACCAGCAAACACGTCTGGGGGCGATTTTGAATGAGACTGGCGATGTTATTTCCGATATTGCGCTCTATTTa                                                                                                      >  1:1313555/1‑150 (MQ=255)

                                                 tctTTTTGCTATTGCCCATCGTGCTTTTTATCCGTATGGCGCTCAATGCGCTGGATGGCATGTTGGCGCGTGAGTGCAACCAGCAAACACGTCTGGGGGCGATTTTGAATGAGACTGGCGATGTTATTTCCGATATTGCGCTCTATTTAc                                                                                                     <  2:1255410/150‑1 (MQ=255)

                                                  ctTTTTGCTATTGCCCATCGTGCTTTTTATCCGTATGGCGCTCAATGCGCTGGATGGCATGTTGGCGCGTGAGTGCAACCAGCAAACACGTCTGGGGGCTATTTTGAATGAGACTGGCGATGTTATTTCCGATATTGCGCTCTATTTAcc                                                                                                    >  2:3241531/1‑150 (MQ=255)

                                                  ctTTTTGCTATTGCCCATCGTGCTTTTTATCCGTATGGCGCTCAATGCGCTGGATGGCATGTTGGCGCGTGAGTGCAACCAGCAAACACGTCTGGGGGCGATTTTGAATGAGACTGGCGATGTTATTTCCGATATTGCGCTCTATTTAcc                                                                                                    >  1:2836142/1‑150 (MQ=255)

                                                  ctTTTTGCTATTGCCCATCGTGCTTTTTATCCGTATGGCGCTCAATGCGCTGGATGGCATGTTGGCGCGTGAGTGCAACCAGCAAACACGTCTGGGGGCGATTTTGAATGAGACTGGCGATGTTATTTCCGATATTGCGCTCTATTTAcc                                                                                                    >  2:3728747/1‑150 (MQ=255)

                                                    ttttGCTATTGCCCATCGTGCTTTTTATCCGTATGGCGCTCAATGCGCTGGATGGCATGTTGGCGCGTGAGTGCAACCAGCAAACACGTCTGGGGGCTATTTTGAATGAGACTGGCGATGTTATTTCCGATATTGCGCTCTATTTACCat                                                                                                  >  1:4174880/1‑150 (MQ=255)

                                                      ttGCTATTGCCCATCGTGCTTTTTATCCGTATGGCGCTCAATGCGCTGGATGGCATGTTGGCGCGTGAGTGCAACCAGCAAACACGTCTGGGGGCGATTTTGAATGAGACTGGCGATGTTATTTCCGATATTGCGCTCTATTTACCattt                                                                                                >  1:4655615/1‑150 (MQ=255)

                                                      ttGCTATTGCCCATCGTGCTTTTTATCCGTATGGCGCTCAATGCGCTGGATGGCATGTTGGCGCGTGAGTGCAACCAGCAAACACGTCTGGGGGCGATTTTGAATGAGACTGGCGATGTTATTTCCGATATTGCGCTCTATTTACCattt                                                                                                >  1:4655528/1‑150 (MQ=255)

                                                      ttGCTATTGCCCATCGTGCTTTTTATCCGTATGGCGCTCAATGCGCTGGATGGCATGTTGGCGCGTGAGTGCAACCAGCAAACACGTCTGGGGGCGATTTTGAATGAGACTGGCGATGTTATTTCCGATATTGCGCTCTATTTACCatta                                                                                                <  1:3063314/150‑2 (MQ=255)

                                                       tGCTATTGCCCATCGTGCTTTTTATCCGTATGGCGCTCAATGCGCTGGATGGCATGTTGGCGCGTGAGTGCAACCAGCAAACACGTCTGGGGGCGATTTTGAATGAGACTGGCGATGTTATTTCCGATATTGCGCTCTATTTACCatttt                                                                                               >  2:1212486/1‑150 (MQ=255)

                                                        gCTATTGCCCATCGTGCTTTTTATCCGTATGGCGCTCAATGCGCTGGATGGCATGTTGGCGCGTGAGTGCAACCAGCAAACACGTCTGGGGGCGATTTTGAATGAGACTGGCGATGTTATTTCCGATATTGCGCTCTATTTACCattttt                                                                                              <  1:4963920/150‑1 (MQ=255)

                                                         cTATTGCCCATCGTGCTTTTTATCCGTATGGCGCTCAATGCGCTGGATGGCATGTTGGCGCGTGAGTGCACCCAGCAAACACGTCTGGGGGCGATTTTGAATGAGACTGGCGATGTTATTTCCGATATTGCGCTCTATTTACCattttta                                                                                             >  2:4345234/1‑150 (MQ=255)

                                                         cTATTGCCCATCGTGCTTTTTATCCGTATGGCGCTCAATGCGCTGGATGGCATGTTGGCGCGTGAGTGCAACCAGCAAACACGTCTGGGGGCGATTTTGAATGAGACTGGCGATGTTATTTCCGATATTGCGCTCTATTTACCattttta                                                                                             >  2:2824125/1‑150 (MQ=255)

                                                          tATTGCCCATCGTGCTTTTTATCCGTATGGCGCTCAATGCGCTGGATGGCATGTTGGCGCGTGAGTGCAACCAGCAAACACGTCTGGGGGCGATTTTGAATGAGACTGGCGATGTTATTTCCGATATTGCGCTCTATTTACCatttttat                                                                                            <  1:159840/150‑1 (MQ=255)

                                                             tGCCCATCGTGCTTTTTATCCGTATGGCGCTCAATGCGCTGGATGGCATGTTGGCGCGTGAGTGCAACCAGCAAACACGTCTGGGGGCGATTTTGAATGAGACTGGCGATGTTATTTCCGATATTGCGCTCTATTTACCatttttatttt                                                                                         >  1:2587073/1‑150 (MQ=255)

                                                             tGCCCATCGTGCTTTTTATCCGTATGGCGCTCAATGCGCTGGATGGCATGTTGGCGCGTGAGTGCAACCAGCAAACACGTCTGGGGGCGATTTTGAATGAGACTGGCGATGTTATTTCCGATATTGCGCTCTATTTACCagttttatttt                                                                                         >  2:833611/1‑150 (MQ=255)

                                                             tGCCCATCGTGCTTTTTATCCGTATGGCGCTCAATGCGCTGGATGGCATGTTGGCGCGTGAGTGCAACCAGCAAACACGTCTCCCGGCGATTTTGAATGAGACTGGCGATGTTATTTCCGATATTGCGCTCTATTTACCatttttatttt                                                                                         >  1:1612900/1‑150 (MQ=255)

                                                              gCCCATCGTGCTTTTTATCCGTATGGCGCTCAATGCGCTGGATGGCATGTTGGCGCGTGAGTGCAACCAGCAAACACGTCTGGGGGCGATTTTGAATGAGACTGGCGATGTTATTTCCGATATTGCGCTCTATTTACCatttttattttt                                                                                        >  2:4789051/1‑150 (MQ=255)

                                                                    cGTGCTTTTTATCCGTATGGCGCTCAATGCGCTGGATGGCATGTTGGCGCGTGAGTGCAACCAGCAAACACGTCTGGGGGCGATTTTGAATGAGACTGGCGATGTTATTTCCGATATTGCGCTCTATTTACCATTTTTATTTTTACCGGa                                                                                  >  1:328803/1‑150 (MQ=255)

                                                                    cGTGCTTTTTATCCGTATGGCGCTCAATGCGCTGGATGGCATGTTGGCGCGTGAGTGCAACCAGCAAACACGTCTGGGGGCGATTTTGAATGAGACTGGCGATGTTATTTCCGATATTGCGCTCTATTTACCATTTTTATTTTTACCGGa                                                                                  >  1:2497035/1‑150 (MQ=255)

                                                                    cGTGCTTTTTATCCGTATGGCGCTCAATGCGCTGGATGGCATATTGGCGCGTGAGTGCAACCAGCAAACACGTCTGGGGGCGATTTTGAATGAGACTGGCGATGTTATTTCCGATATTGCGCTCTATTTACCATTTTTATTTTTACCGGa                                                                                  <  2:266677/150‑1 (MQ=255)

                                                                     gTGCTTTTTATCCGTATGGCGCTCAATGCGCTGGATGGCATGTTGGCGCGTGAGTGCAACCAGCAAACACGTCTGGGGGCGATTTTGAATGAGACTGGCGATGTTATTTCCGATATTGCGCTCTATTTACCATTTTTATTTTTACCGGaa                                                                                 <  2:2608814/150‑1 (MQ=255)

                                                                       gCTTTTTATCCGTATGGCGCTCAATGCGCTGGATGGCATGTTGGCGCGTGAGTGCAACCAGCAAACACGTCTGGGGGCGATTTTGAATGAGACTGGCGATGTTATTTCCGATATTGCGCTCTATTTACCATTTTTATTTTTACCGGAAAg                                                                               >  2:3644026/1‑150 (MQ=255)

                                                                        cTTTTTATCCGTATGGCGCTCAATGCGCTGGATGGCATGTTGGCGCGTGAGTGCAACCAGCAAACACGTCTGGGGGCGATTTTGAATGAGACTGGCGATGTTATTTCCGATATTGCGCTCTATTTACCATTTTTATTTTTACCGGAAAGt                                                                              <  1:2120163/150‑1 (MQ=255)

                                                                        cTTTTTATCCGTATGGCGCTCAATGCGCTGGATGGCATGTTGGCGCGTGAGTGCAACCAGCAAACACGTCTGGGGGCGATTTTGAATGAGACTGGCGATGTTATTTCCGATATTGCGCTCTATTTACCATTTTTATTTTTACCGGAAAGt                                                                              <  1:1816128/150‑1 (MQ=255)

                                                                            ttATCCGTATGGCGCTCAATGCGCTGGATGGCATGTTGGCGCGTGAGTGCAACCAGCAAACACGTCTGGGGGCGATTTTGAATGAGACTGGCGATGTTATTTCCGATATTGCGCTCTATTTACCATTTTTATTTTTACCGGAAAGTAACg                                                                          <  2:1963909/150‑1 (MQ=255)

                                                                              aTCCGTATGGCGCTCAATGCGCTGGATGGCATGTTGGCGCGTGAGTGCAACCAGCAAACACGTCTGGGGGCGATTTTGAATGAGACTGGCGATGTTATTTCCGATATTGCGCTCTATTTACCATTTTTATTTTTACCGGAAAGTAACGCa                                                                        <  1:2874821/150‑1 (MQ=255)

                                                                                 cGTATGGCGCTCAATGCGCTGGATGGCATGTTGGCGCGTGAGTGCAACCAGCAAACACGTCTGGGGGCTATTTTGAATGAGACTGGCGATGTTATTTCCGATATTGCGCTCTATTTACCATTTTTATTTTTACCGGAAAGTAACGCATCa                                                                     <  1:2920231/150‑1 (MQ=255)

                                                                                   tATGGCGCTCAATGCGCTGGATGGCATGTTGGCGCGTGAGTGCAACCAGCAAACACGTCTGGGGGCGATTTTGAATGAGACTGGCGATGTTATTTCCGATATTGCGCTCTATTTACCATTTTTATTTTTACCGGAAAGTAACGCATCACt                                                                   >  1:3839139/1‑150 (MQ=255)

                                                                                    aTGGCGCTCAATGCGCTGGATGGCATGTTGGCGCGTGAGTGCAACCAGCAAACACGTCTGGGGGCTATTTTGAATGAGACTGGCGATGTTATTTCCGATATTGCGCTCTATTTACCATTTTTATTTTTACCGGAAAGTAACGCATCACTc                                                                  >  2:2382966/1‑150 (MQ=255)

                                                                                    aTGGCGCTCAATGCGCTGGATGGCATGTTGGCGCGTGAGTGCAACCAGCAAACACGTCTGGGGGCGATTTTGAATGAGACTGGCGATGTTATTTCCGATATTGCGCTCTATTTACCATTTTTATTTTTACCGGAAAGTAACGCATCACTc                                                                  <  2:385184/150‑1 (MQ=255)

                                                                                      ggCGCTCAATGCGCTGGATGGCATGTTGGCGCGTGAGTGCAACCAGCAAACACGTCTGGGGGCTATTTTGAATGAGACTGGCGATGTTATTTCCGATATTGCGCTCTATTTACCATTTTTATTTTTACCGGAAAGTAACGCATCACTCGt                                                                <  2:1057357/150‑1 (MQ=255)

                                                                                      ggCGCTCAATGCGCTGGATGGCATGTTGGCGCGTGAGTGCAACCAGCAAACACGTCTGGGGGCTATTTTGAATGAGACTGGCGATGTTATTTCCGATATTGCGCTCTATTTACCATTTTTATTTTTACCGGAAAGTAACGCATCACTCGt                                                                <  2:3478771/150‑1 (MQ=255)

                                                                                       gcgcTCAATGCGCTGGATGGCATGTTGGCGCGTGAGTGCAACCAGCAAACACGTCTGGGGGCGATTTTGAATGAGACTGGCGATGTTATTTCCGATATTGCGCTCTATTTACCATTTTTATTTTTACCGGAAAGTAACGCATCACTCGTg                                                               <  2:1590822/150‑1 (MQ=255)

                                                                                        cgcTCAATGCGCTGGATGGCATGTTGGCGCGTGAGTGCAACCAGCAAACACGTCTGGGGGCGATTTTGAATGAGACTGGCGATGTTATTTCCGATATTGCGCTCTATTTACCATTTTTATTTTTACCGGAAAGTAACGCATCACTCGTGa                                                              >  1:3092465/1‑150 (MQ=255)

                                                                                          cTCAATGCGCTGGATGGCATGTTGGCGCGTGAGTGCAACCAGCAAACACGTCTGGGGGCGATTTTGAATGAGACTGGCGATGTTATTTCCGATATTGTGCTCTATTTACCATTTTTATTTTTACCGGAAAGTAACGCATCACTCGTGATa                                                            <  1:1961147/150‑1 (MQ=255)

                                                                                          cTCAATGCGCTGGATGGCATGTTGGCGCGTGAGTGCAACCAGCAAACACGTCTGGGGGCGATTTTGAATGAGACTGGCGATGTTATTTCCGATATTGCGCTCTATTTACCATTTTTATTTTTACCGGAAAGTAACGCATCACTCGTGATa                                                            <  1:1131592/150‑1 (MQ=255)

                                                                                          cTCAATGCGCTGGATGGCATGTTGGCGCGTGAGTGCAACCAGCAAACACGTCTGGGGGCGATTTTGAATGAGACTGGCGATGTTATTTCCGATATTGCGCTCTATTTACCATTTTTATTTTTACCGGAAAGTAACGCATCACTCGTGATa                                                            <  1:4106322/150‑1 (MQ=255)

                                                                                          cTCAATGCGCTGGATGGCATGTTGGCGCGTGAGTGCAACCAGCAAACACGTCTGGGGGCGATTTTGAATGAGACTGGCGATGTTATTTCCGATATTGCGCTCTATTTACCATTTTTATTTTTACCGGAAAGTAACGCATCACTCGTGATa                                                            <  2:1991768/150‑1 (MQ=255)

                                                                                            cAATGCGCTGGATGGCATGTTGGCGCGTGAGTGCAACCAGCAAACACGTCTGGGGGCTATTTTGAATGAGACTGGCGATGTTATTTCCGATATTGCGCTCTATTTACCATTTTTATTTTTACCGGAAAGTAACGCATCACTCGTGATACt                                                          <  1:910312/150‑1 (MQ=255)

                                                                                            cAATGCGCTGGATGGCATGTTGGCGCGTGAGTGCAACCAGCAAACACGTCTGGGGGCTATTTTGAATGAGACTGGCGATGTTATTTCCGATATTGCGCTCTATTTACCATTTTTATTTTTACCGGAAAGTAACGCATCACTCGTGATACt                                                          <  1:4842914/150‑1 (MQ=255)

                                                                                              aTGCGCTGGATGGCATGTTGGCGCGTGAGTGCAACCAGCAAACACGTCTGGGGGCGATTTTGAATGAGACTGGCGATGTTATTTCCGATATTGCGCTCTATTTACCATTTTTATTTTTACCGGAAAGTAACGCATCACTCGTGATACTCa                                                        >  2:892813/1‑150 (MQ=255)

                                                                                                     ggATGGCATGTTGGCGCGTGAGTGCAACCAGCAAACACGTCTGGGGGCGATTTTGAATGAGACTGGCGATGTTATTTCCGATATTGCGCTCTATTTACCATTTTTATTTTTACCGGAAAGTAACGCATCACTCGTGATACTCATGTTAtt                                                 <  1:4775376/150‑1 (MQ=255)

                                                                                                       aTGGCATGTTGGCGCGTGAGTGCAACCAGCAAACACGTCTGGGGGCGATTTTGAATGAGACTGGCGATGTTATTTCCGATATTGCGCTCTATTTACCATTTTTATTTTTACCGGAAAGTAACGCATCACTCGTGATACTCATGTTAtttt                                               >  1:4341257/1‑150 (MQ=255)

                                                                                                         ggCATGTTGGCGCGTGAGTGCAACCAGCAAACACGTCTGGGGGCGATTTTGAATGAGACTGGCGATGTTATTTCCGATATTGCGCTCTATTTACCATTTTTATTTTTACCGGAAAGTAACGCATCACTCGTGATACTCATGTTATTTTGc                                             <  2:1109741/150‑1 (MQ=255)

                                                                                                             tGTTGGCGCGTGAGTGCAACCAGCAAACACGTCTGGGGGCGATTTTGAATGAGACTGGCGATGTTATTTCCGATATTGCGCTCTATTTACCATTTTTATTTTTACCGGAAAGTAACGCATCACTCGTGATACTCATGTTATTTTGCACCa                                         <  1:892523/150‑1 (MQ=255)

                                                                                                               ttGGCGCGTGAGTGCAACCAGCAAACACGTCTGGGGGCTATTTTGAATGAGACTGGCGATGTTATTTCCGATATTGCGCTCTATTTACCATTTTTATTTTTACCGGAAAGTAACGCATCACTCGTGATACTCATGTTATTTTGCACCata                                       <  1:1879152/150‑1 (MQ=255)

                                                                                                                       tGAGTGCAACCAGCAAACACGTCTGGGGGCGATTTTGAATGAGACTGGCGATGTTATTTCCGATATTGCGCTCTATTTACCATTTTTATTTTTACCGGAAAGTAACGCATCACTCGTGATACTCATGTTATTTTGCACCATATTGACCGa                               >  2:4059779/1‑150 (MQ=255)

                                                                                                                         aGTGCAACCAGCAAACACGTCTGGGGGCGATTTTGAATGAGACTGGCGATGTTATTTCCGATATTGCGCTCTATTTACCATTTTTATTTTTACCGGAAAGTAACGCATCACTCGTGATACTCATGTTATTTTGCACCATATTGACCGAGt                             >  2:4979199/1‑150 (MQ=255)

                                                                                                                           tGCAACCAGCAAACACGTCTGGGGGCGATTTTGAATGAGACTGGCGATGTTATTTCCGATATTGCGCTCTATTTACCATTTTTATTTTTACCGGAAAGTAACGCATCACTCGTGATACTCATGTTATTTTGCACCATATTGACCGAGttt                           <  1:1883878/150‑1 (MQ=255)

                                                                                                                           tGCAACCAGCAAACACGTCTGGGGGCGATTTTGAATGAGACTGGCGATGTTATTTCCGATATTGCGCTCTATTTACCATTTTTATTTTTACCGGAAAGTAACGCATCACTCGTGATACTCATGTTATTTTGCACCATATTGACCGAGttt                           <  1:4484378/150‑1 (MQ=255)

                                                                                                                            gCACCCAGCAAACCCGTCTGGGGGCTATGTTGACTGAGACTGGCGATGTTATGTCCGATATTGCGCTCTATTTACCATTTTTATTTTTACCGGAAAGTAACGCATCACTCGTGATACTCATGTTATTTTGCACCATATTGACCGAGtttt                          <  2:3468762/150‑1 (MQ=255)

                                                                                                                             cAACCAGCAAACACGTCTGGGGGCGATTTTGAATGAGACTGGCGATGTTATTTCCGATATTGCGCTCTATTTACCATTTTTATTTTTACCGGAAAGTAACGCATCACTCGTGATACTCATGTTATTTTGCACCATATTGACCGAGTTTTg                         >  2:3921463/1‑150 (MQ=255)

                                                                                                                             cAACCAGCAAACACGTCTGGGGGCGATTTTGAATGAGACTGGCGATGTTATTTCCGATATTGCGCTCTATTTACCATTTTTATTTTTACCGGAAAGTAACGCATCACTCGTGATACTCATGTTATTTTGCACCATATTGACCGAGTTTTg                         >  2:5143949/1‑150 (MQ=255)

                                                                                                                                   gCAAACACGTCTGGGGGCGATTTTGAATGAGACTGGCGATGTTATTTCCGATATTGCGCTCTATTTACCATTTTTATTTTTACCGGAAAGTAACGCATCACTCGTGATACTCATGTTATTTTGCACCTTATTGACCGAGTTTTGCGGttt                   <  1:3728747/150‑1 (MQ=255)

                                                                                                                                          cGTCTGGGGGCGATTTTGAATGAGACTGGCGATGTTATTTCCGATATTGCGCTCTATTTACCATTTTTATTTTTACCGGAAAGTAACGCATCACTCGTGATACTCATGTTATTTTGCACCATATTGACCGAGTTTTGCGGTTTACTcgcg            >  2:241803/1‑150 (MQ=255)

                                                                                                                                           gTCTGGGGGCGATTTTGAATGAGACTGGCGATGTTATTTCCGATATTGCGCTCTATTTACCATTTTTATTTTTACCGGAAAGTAACGCATCACTCGTGATACTCATGTTATTTTGCACCATATTGACCGAGTTTTGCGGTTTACTCgcgc           <  1:623751/150‑1 (MQ=255)

                                                                                                                                             cTGGGGGCTATTTTGAATGAGACTGGCGATGTTATTTCCGATATTGCGCTCTATTTACCATTTTTATTTTTACCGGAAAGTAACGCATCACTCGTGATACTCATGTTATTTTGCACCATATTGACCGAGTTTTGCGGTTTACTCGCGCAg         >  2:2138840/1‑150 (MQ=255)

                                                                                                                                               gggggCTATTTTGAATGAGACTGGCGATGTTATTTCCGATATTGCGCTCTATTTACCATTTTTATTTTTACCGGAAAGTAACGCATCACTCGTGATACTCATGTTATTTTGCACCATATTGACCGAGTTTTGCGGTTTACTCGCGCAGAc       >  1:688541/1‑150 (MQ=255)

                                                                                                                                               gggggCTATTTTGAATGAGACTGGCGATGTTATTTCCGATATTGCGCTCTATTTACCATTTTTATTTTTACCGGAAAGTAACGCATCACTCGTGATACTCATGTTATTTTGCACCATATTGACCGAGTTTTGCGGTTTACTCGCGCAGAc       <  2:4882057/150‑1 (MQ=255)

                                                                                                                                               gggggCGATTTTGAATGAGACTGGCGATGTTATTTCCGATATTGCGCTCTATTTACCATTTTTATTTTTACCGGAAAGTAACGCATCACTCGTGATACTCATGTTATTTTGCACCATATTGACCGAGTTTTGCGGTTTACTCGCGCAGAc       <  1:3644156/150‑1 (MQ=255)

                                                                                                                                                ggggCTATTTTGAATGAGACTGGCGATGTTATTTCCGATATTGCGCTCTATTTACCATTTTTATTTTTACCGGAAAGTAACGCATCACTCGTGATACTCATGTTATTTTGCACCATATTGACCGAGTTTTGCGGTTTACTCGCGCAGACg      <  1:5156020/150‑1 (MQ=255)

                                                                                                                                                   gCGATTTTGAATGAGACTGGCGATGTTATTTCCGATATTGCGCTCTATTTACCATTTTTATTTTTACCGGAAAGTAACGCATCACTCGTGATACTCATGTTATTTTGCACCATATTGACCGAGTTTTGCGGTTTACTCGCGCAGACGAtt   <  2:3061509/150‑1 (MQ=255)

                                                                                                                                                    cGATTTTGAATGAGACTGGCGATGTTATTTCCGATATTGCGCTCTATTTACCATTTTTATTTTTACCGGAAAGTAACGCATCACTCGTGATACTCATGTTATTTTGCACCATATTGACCGAGTTTTGCGGTTTACTCGCGCAGACGATTa  <  2:3663757/150‑1 (MQ=255)

CTTTCTCTTCTTACCGGATTGCTGTTGATGTTGGCGGCACAACCCATCCTCTTTTTGCTATTGCCCATCGTGCTTTTTATCCGTATGGCGCTCAATGCGCTGGATGGCATGTTGGCGCGTGAGTGCAACCAGCAAACACGTCTGGGGGCGATTTTGAATGAGACTGGCGATGTTATTTCCGATATTGCGCTCTATTTACCATTTTTATTTTTACCGGAAAGTAACGCATCACTCGTGATACTCATGTTATTTTGCACCATATTGACCGAGTTTTGCGGTTTACTCGCGCAGACGATTA  >  CP009273/1471989‑1472286

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`