Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,500,047 A→T 5.1% F97L (TTT→TTA)  ydcO ← BenE family inner membrane putative transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,500,0470AT 5.1% 852.8 / 23.6 244F97L (TTT→TTA) ydcOBenE family inner membrane putative transporter
Reads supporting (aligned to +/- strand):  ref base A (134/97);  new base T (8/5);  total (142/102)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 255 total aligned reads displayed.

TGACCGTCCAGACTGGCAAACGCCTGTAAACCAAAGCGTAATAAAATCCCGGCAAGCATTGCCGCCGCAAGCGAGTGCGGAATAATGCGCATCAGACGAGCAAAGAGTCCCGTTATGCCGCAGAGGACTATTAGCGCGTTGGTGACAATAAAAACGCCGATGGCTTCGTTAAGTGTTAGTCCCTGCAATCCGGTGACCAACAAAGCCGCGCCAGGCGTTGACCATGCGGTGAGAACAGGTACGCGATACCATAATGTCAGAGTCAGCGTACTGACGCCCATTGCCAGCCCCAGCGCCGT  >  CP009273/1499898‑1500196
                                                                                                                                                     |                                                                                                                                                     
tGACCGTCCAGACTGGCAAACGCCTGTAAACCAAAGCGTAATAAAATCCCGGCAAGCATTGCCGCCGCAAGCGAGTGCGGAATAATGCGCATCAGACGAGCAAAGAGTCCCGTTATGCCGCAGAGGACTATTAGCGCGTTGGTGACAATa                                                                                                                                                       <  1:1381696/150‑1 (MQ=255)
  aCCGTCCAGACTGGCAAACGCCTGTAAACCAAAGCGTAATAAAATCCCGGCAAGCATTGCCGCCGCAAGCGAGTGCGGAATAATGCGCATCAGACGAGCAAAGAGTCCCGTTATGCCACAGAGGACTATTAGCGCGTTGGTGACAATaaa                                                                                                                                                     >  2:4808795/1‑150 (MQ=255)
   ccGTCCAGACTGGCAAACGCCTGTAAACCAAAGCGTAATAAAATCCCGGCAAGCATTGCCGCCGCAAGCGAGTGCGGAATAATGCGCATCAGACGAGCAAAGAGTCCCGTTATGCCGCAGAGGACTATTAGCGCGTTGGTGACAATaaaa                                                                                                                                                    >  2:2226781/1‑150 (MQ=255)
     gTCCAGACTGGCAAACGCCTGTAAACCAAAGCGTAATAAAATCCCGGCAAGCATTGCCGCCGCAAGCGAGTGCGGAATAATGCGCATCAGACGAGCAAAGAGTCCCGTTATGCCGCAGAGGACTATTAGCGCGTTGGTGACAATAAAAAc                                                                                                                                                  >  1:492463/1‑150 (MQ=255)
     gTCCAGACTGGCAAACGCCTGTAAACCAAAGCGTAATAAAATCCCGGCAAGCATTGCCGCCGCAAGCGAGTGCGGAATAATGCGCATCAGACGAGCAAAGAGTCCCGTTATGCCGCAGAGGACTATTAGCGCGTTGGTGACAATAAAAAc                                                                                                                                                  <  1:5065867/150‑1 (MQ=255)
     gTCCAGACTGGCAAACGCCTGTAAACCAAAGCGTAATAAAATCCCGGCAAGCATTGCCGCCGCAAGCGAGTGCGGAATAATGCGCATCAGACGAGCAAAGAGTCCCGTTATGCCGCAGAGGACTATTAGCGCGTTGGTGACAATAAAAAc                                                                                                                                                  <  2:1669701/150‑1 (MQ=255)
     gTCCAGACTGGCAAACGCCTGTAAACCAAAGCGTAATAAAATCCCGGCAAGCATTGCCGCCGCAAGCGAGTGCGGAATAATGCGCATCAGACGAGCAAAGAGTCCCGTTATGCCGCAGAGGACTATTAGCGCGTTGGTGACAATAAAAAc                                                                                                                                                  <  1:239419/150‑1 (MQ=255)
       ccAGACTGGCAAACGCCTGTAAACCAAAGCGTAATAAAATCCCGGCAAGCATTGCCGCCGCAAGCGAGTGCGGAATAATGCGCATCAGACGAGCAAAGAGTCCCGTTATGCCGCAGAGGACTATTAGCGCGTTGGTGACAATAAAAACGc                                                                                                                                                <  2:3881826/150‑1 (MQ=255)
         aGACTGGCAAACGCCTGTAAACCAAAGCGTAATAAAATCCCGGCAAGCATTGCCGCCGCAAGCGAGTGCGGAATAATGCGCATCAGACGAGCAAAGAGTCCCGTTATGCCGCAGAGGACTATTAGCGCGTTGGTGACAATAAAAACGCCg                                                                                                                                              <  2:2917694/150‑1 (MQ=255)
         aGACTGGCAAACGCCTGTAAACCAAAGCGTAATAAAATCCCGGCAAGCATTGCCGCCGCAAGCGAGTGCGGAATAATGCGCATCAGACGAGCAAAGAGTCCCGTTATGCCGCAGAGGACTATTAGCGCGTTGGTGACAATAAAAACGCCg                                                                                                                                              >  1:2126769/1‑150 (MQ=255)
          gACTGGCAAACGCCTGTAAACCAAAGCGTAATAAAATCCCGGCAAGCATTGCCGCCGCAAGCGAGTGCGGAATAATGCGCATCAGACGAGCAAAGAGTCCCGTTATGCCGCAGAGGACTATTAGCGCGTTGGTGACAATAAAAACGCCGa                                                                                                                                             >  1:2224958/1‑150 (MQ=255)
            cTGGCAAACGCCTGTAAACCAAAGCGTAATAAAATCCCGGCAAGCATTGCCGCCGCAAGCGAGTGCGGAATAATGCGCATCAGACGAGCAAAGAGTCCCGTTATGCCGCAGAGGACTATTAGCGCGTTGGTGACAATAAAAACGCCGATg                                                                                                                                           >  2:300006/1‑150 (MQ=255)
                 aaaCGCCTGTAAACCAAAGCGTAATAAAATCCCGGCAAGCATTGCCGCCGCAAGCGAGTGCGGAATAATGCGCATCAGACGAGCAAAGAGTCCCGTTATGCCGCAGAGGACTATTAGCGCGTTGGTGACAATTAAAACGCCGATGGCTTc                                                                                                                                      >  2:3169370/1‑150 (MQ=255)
                 aaaCGCCTGTAAACCAAAGCGTAATAAAATCCCGGCAAGCATTGCCGCCGCAAGCGAGTGCGGAATAATGCGCATCAGACGAGCAAAGAGTCCCGTTATGCCGCAGAGGACTATTAGCGCGTTGGTGACAATAAAAACGCCGATGGCTTc                                                                                                                                      >  2:585078/1‑150 (MQ=255)
                  aaCGCCTGTAAACCAAAGCGTAATAAAATCCCGGCAAGCATTGCCGCCGCAAGCGAGTGCGGAATAATGCGCATCAGACGAGCAAAGAGTCCCGTTATGCCGCAGAGGACTATTAGCGCGTTGGTGACAATAAAAACGCCGATGGCTTCg                                                                                                                                     >  1:1736463/1‑150 (MQ=255)
                     gCCTGTAAACCAAAGCGTAATAAAATCCCGGCAAGCATTGCCGCCGCAAGCGAGTGCGGAATAATGCGCATCAGACGAGCAAAGAGTCCCGTTATGCCGCAGAGGACTATTAGCGCGTTGGTGACAATAAAAACGCCGATGGCTTCGTTa                                                                                                                                  >  1:1465482/1‑150 (MQ=255)
                       cTGTAAACCAAAGCGTAATAAAATCCCGGCAAGCATTGCCGCCGCAAGCGAGTGCGGAATAATGCGCATCAGACGAGCAAAGAGTCCCGTTATGCCGCAGAGGAGTATTAGCGCGTTGGTGACAATTAAAACGCCGATGGCTTCGTTAAg                                                                                                                                >  2:1939696/1‑150 (MQ=255)
                        tGTAAACCAAAGCGTAATAAAATCCCGGCAAGCATTGCCGCCGCAAGCGAGTGCGGAATAATGCGCATCAGACGAGCAAAGAGTCCCGTTATGCCGCTGAGGACTATTAGCGCGTTGGTGACAATAAAAACGCCGATGGCTTCGTTAAgt                                                                                                                               >  2:3065089/1‑150 (MQ=255)
                          tAAACCAAAGCGTAATAAAATCCCGGCAAGCATTGCCGCCGCAAGCGAGTGCGGAATAATGCGCATCAGACGAGCAAAGAGTCCCGTTATGCCGCAGAGGACTATTAGCGCGTTGGTGACAATAAAAACGCCGATGGCTTCGTTAAgtgt                                                                                                                             >  2:3304877/1‑150 (MQ=255)
                             aCCAAAGCTTAATAACCTCCCGGCAAGCATTGCCGCCGAACGCGAGTGCGTAATATTGCGCTTCAGAGTCGAAAAGAGTCCGGTTATGCCGCAGTGGCCTTTTAGCGCGTTGGTGCCAATAAAAACGCCGATGGCTTCGTTAAGTGTTAg                                                                                                                          <  2:3867861/150‑1 (MQ=255)
                              ccAAAGCGTAATAAACTCCCGGCACGCATTGCCGCCGCAAGCGAGTGCGGAATAATGCGCATCCGCCGAGCAACGAGTCCCGTTATGCCGCAGAGGACTATTCGCGCGTGGGTGACAATAAACACGCCGATGGCTTCGTTAAGTGTTAGt                                                                                                                         <  2:4081680/150‑1 (MQ=255)
                                aaaGCGTAATAAAATCCCGGCAAGCATTGCCGCCGCAAGCGAGTGCGGAATAATGCGCATCAGACGAGCAAAGAGTCCCGTTATGCCGCAGAGGACTATTAGTGCGTGGGTGACAATAAAAACGCCGATGGCTTCGTTAAGTGTTAGTcc                                                                                                                       >  2:500133/1‑150 (MQ=255)
                                aaaGCGTAATAAAATCCCGGCAAGCATTGCCGCCGCAAGCGAGTGCGGAATAATGCGCATCAGACGAGCAAAGAGTCCCGTTATGCCGCAGAGGACTATTAGCGCGTTGGTGACACTAAAAACGCCGATGGCTTCGTTAAGTGTTAGTcc                                                                                                                       >  2:907824/1‑150 (MQ=255)
                                aaaGCGTAATAAAATCCCGGCAAGCATTGCCGCCGCAAGCGAGTGCGGAATAATGCGCATCAGACGAGCAAAGAGTCCCGTTATGCCGCAGAGGACTATTAGCGCGTTGGTGACAATAAAAACGCCGATGGCTTCGTTAAGTGTTAGTcc                                                                                                                       >  2:1207542/1‑150 (MQ=255)
                                aaaGCGTAATAAAATCCCGGCAAGCATTGCCGCCGCAAGCGAGTGCGGAATAATGCGCATCAGACGAGCAAAGACTCCCGTTATGCCGCAGAGGACTATTAGTGCGTTGGTGACAATAAAAACGCCGATGGCTTCGTTAAGTGTTAGTcc                                                                                                                       >  2:3443032/1‑150 (MQ=255)
                                 aaGCGTAATAAAATCCCGGCAAGCATTGCCGCCGCAAGCGAGTGCGGAATAATGCGCATCAGACGAGCAAAGAGTCCCGTTATGCCGCAGAGGACTATTAGCGCGTTGGTGACAATAAAAACGCCGATGGCTTCGTTAAGTGTTAGTccc                                                                                                                      >  2:1841504/1‑150 (MQ=255)
                                 aaGCGTAATAAAATCCCGGCAAGCATTGCCGCCGCAAGCGAGTGCGGAATAATGCGCATCAGACGAGCAAAGAGTCCCGTTATGCCGCAGAGGACTATTAGCGCGTTGGTGACAATAAAAACGCCGATGGCTTCGTTAAGTGTTAGTccc                                                                                                                      >  2:4430985/1‑150 (MQ=255)
                                     gTAATAAAATCCCGGCAAGCATTGCCGCCGCAAGCGAGTGCGGAATAATGCGCATCAGACGAGCAAAGAGTCCCGTTATGCCGCAGAGGACTATTAGCGCGTTGGTGACAATAAAAACGCCGATGGCTTCGTTAAGTGTTAGTCCCTGCa                                                                                                                  >  2:4836328/1‑150 (MQ=255)
                                        ataaAATCCCGGCAAGCTTTGCCGCCGCAAGCTAGTGCGTAATAATGCGCATCAGACGACCAAATTGTCCCTTTATGCCGCATAGGAATATTAGCGCGTTGTTGAAAATAAAAACGCCGATGGCTTCGTTAAGTGTTAGTCCCTGCAATc                                                                                                               <  1:1677075/150‑1 (MQ=255)
                                        ataaAATCCCGGCAAGCATTGCCGCCGCAAGCGAGTGCGGAATAATGCGCATCAGACGAGCAAAGAGTCCCGTTATGCCGCAGAGGACTATTAGCGCGTTGGTGACAATAAAAACGCCGATGGCTTCGTTAAGTGTTAGTCCCTGCAATc                                                                                                               >  1:4843/1‑150 (MQ=255)
                                               cccGGCAAGCATTGCCGCCGCACGCGAGTGCGTAATAATGCGCATCCGACGAGCAAAGAGTCCCGTTTTGCCGCAGAGGACTATTAGCGCGTTGGTGACAATAAAAACGCCGATGGCTTCGTTAAGTGTTAGTCCCTGCAATCCGGTGAc                                                                                                        <  2:253480/150‑1 (MQ=255)
                                                 cGGCAAGCATTGCCGCCGCAAGCGAGTGCGGAATAATGCGCATCAGACGAGCAAAGAGTCCCGTTATGCCGCAGAGGACTATTAGCGCGTTGGTGACAATAAAAACGCCGATGGCTTCGTTAAGTGTTAGTCCCTGCAATCCGGTGACca                                                                                                      >  2:398548/1‑150 (MQ=255)
                                                  ggCAAGCATTGCCGCCGCAAGCGAGTGCGGAATAATGCGCATCAGACGAGCAAAGAGTCCCGTTATGCCGCAGAGGACTATTAGCGCGTTGGTGACAATAAAAACGCCGATGGCTTCGTTAAGTGTTAGTCCCTGCAATCCGGTGACcaa                                                                                                     >  2:2296942/1‑150 (MQ=255)
                                                     aaGCATTGCCGCCGCAAGCGAGTGCGGACTAATGCGCATCAGACGAGCAAAGAGTCCCGTTATGCCGCAGAGGACTATTAGCGCGTTGGTGACAATAAAAACGCCGATGGCTTCGTTAAGTGTTAGTCCCTGCAATCCGGTGACcaacaa                                                                                                  >  2:4419293/1‑150 (MQ=255)
                                                      aGCATTGCCGCCGCAAGCGAGTGCGGAATAATGCGCATCAGACGAGCAAAGAGTCCCGTTATGCCGCAGAGGACTATTAGCGCGTTGGTGACAATAAAAACGCCGATGGCTTCGTTAAGTGTTAGTCCCTGCAATCCGGTGACCAACaaa                                                                                                 >  1:1265279/1‑150 (MQ=255)
                                                      aGCATTGCCGCCGCAAGCGAGTGCGGAATAATGCGCATCAGACGAGCAAAGAGTCCCGTTATGCCGCAGAGGACTATTAGCGCGTTGGTGACAATAAAAACGCCGATGGCTTCGTTAAGTGTTAGTCCCTGCAATCCGGTGACCAACaaa                                                                                                 >  1:477528/1‑150 (MQ=255)
                                                        cATTGCCGCCGCAAGCGAGTGCGGAATAATGCGCATCAGACGAGCAAAGAGTCCCGTTATGCCGCAGAGGACTATTAGCGCGTTGGTGACAATAAAAACGCCGATGGCTTCGTTAAGTGTTAGTCCCTGCAATCCGGTGACCAACAAAGc                                                                                               >  2:3286283/1‑150 (MQ=255)
                                                         aTTGCCGCCGCAAGCGAGTGCGGAATAATGCGCATCAGACGAGCAAAGAGTCCCGTTATGCCGCAGAGGACTATTAGCGCGTTGGTGACAATAAAAACGCCGATGGCGTCGTTAAGTGTTAGTCCCTGCAATCCGGTGACCAACAAAGcc                                                                                              >  2:1492322/1‑150 (MQ=255)
                                                                   cAAGCGAGTGCGGAATAATGCGCATCAGACGAGCAAAGAGTCCCGTTATGCCGCAGAGGACTATTAGCGCGTTGGTGACAATAAAAACGCCGATGGCTTCGTTAAGTGTTAGTCCCTGCAATCCGGTGACCAACAAAGCCGCGCCAGGCg                                                                                    >  1:4498288/1‑150 (MQ=255)
                                                                    aaGCGAGTGCGGAATAATGCGCATCAGACGAGCAAAGAGTCCCGTTATGCCGCAGAGGACTATTAGCGCGTTGGTGACAATAAAAACGCCGATGGCTTCGTTAAGTGTTAGTCCCTGCAATCCGGTGACCAACAAAGCCGCGCCAGGCGt                                                                                   >  1:4954946/1‑150 (MQ=255)
                                                                         aGTGCGGAATACTGCGCATCAGACGAGCAAAGAGTCCCGTTATGCCGCAGAGGACTATTAGCGCGTTGGTGACAATAAAAACGCCGATGGCTTCGTTAAGTGTTAGTCCCTGCAATCCGGTGACCAACAAAGCCGCGCCAGGCGTTGAcc                                                                              <  2:2572438/150‑1 (MQ=255)
                                                                           tGCGGAATAAGGCGCATCAGACGAGCAAAGAGTCCCGTTATGCCGCAGAGGACTATTAGCGCGTTGGTGACAATAAACACGCCGATGGCTTCGTTAAGTGTTAGTCCCTGCAATCCGGTGACCAACAAAGCCGCGCCAGGCGTTGACCAt                                                                            <  2:5139407/150‑1 (MQ=255)
                                                                            gCGTAATAATGCGAATCAGACTCTCAAATCGTCCGTTTTTGCCGCTGCGGATTATTCGCGGGTTGGTTAAAATAAACACGCCGATGGCTTCGTTAAGTGTTAGTCCCTGCAATCCGGTGACCAACAAAGCCGCGCCAGGCGTTGACCATg                                                                           <  1:662730/150‑1 (MQ=255)
                                                                             cgtaataatGCGCATCAGACTAGCAAAGAGTCCCGTTATGCCGCAGAGGACTATTAGCGCGTTGGTGACAATAAAAACGCCGATGGCTTCGTTAAGTGTTAGTCCCTGCAATCCGGTGACCAACAAAGCCGCGCCAGGCGTTGACCATGc                                                                          <  2:272225/147‑1 (MQ=255)
                                                                              ggAATAATGCGCATCAGACGAGCAAAGAGTCCCGTTATGCCGCAGAGGACTATTAGCGCGTTGGTGACAATAAAAACGCCGATGGCTTCGTTAAGTGTTAGTCCCTGCAATCCGGTGACCAACAAAGCCGCGCCAGGCGTTGACCATGCg                                                                         >  1:3026094/1‑150 (MQ=255)
                                                                               gAATAATGCGCATCAGACGAGCAAAGAGTCCCGTTATGCCGCAGAGGACTATTAGCGCGTTGGTGACAATAAAAACGCCGATGGCTTCGTTAAGTGTTAGTCCCTGCAATCCGGTGACCAACAAAGCCGCGCCAGGCGTTGACCATGCgg                                                                        >  2:2040949/1‑150 (MQ=255)
                                                                                  taatGCGCATCCGCCGACCAACTCGTCCCGTTAGGCCGCAGAGGACTATTCGCGCGTTGGTTACAATAAACACGCCGATGGCTTCGTTAAGTGTTAGTCCCTGCAATCCGGTGACCAACAAAGCCGCGCCAGGCGTTGACCATGCGGTga                                                                     <  2:495235/150‑1 (MQ=255)
                                                                                   acggcccATCACACGAGAACAGTGTCCCGTTCTCGCGCCGAGGACTTTTAGCGCGTTGGTTAAAATAAACACGCCGATGGCTTCGTTAAGTGTTAGTCCCTGCAATCCGGTGACCAACAAAGCCGCGCCAGGCGTTGACCATGCGGTgag                                                                    <  1:992667/144‑1 (MQ=255)
                                                                                   aatGCGCATCAGACGAGCAAAGAGTCCCGTTATGCCGCAGAGGACTATTAGCGCGTTGGTGACAATAAAAACGCCGATGGCTTCGTTAAGTGTTAGTCCCTGCAATCCGGTGACCAACAAAGCCGCGCCAGGCGTTGACCATGCGGTgag                                                                    >  2:3595658/1‑150 (MQ=255)
                                                                                      gcgcATCTCACGAGAAAAGCGTCCCGTTATGCCGCAGTGGACTTTTTGCGGGTTGGTGACAATAAAAACGCCGATGGCTTCGTTAAGTGTTAGTCCCTGCAATCCGGTGACCAACAAAGCCGCGCCAGGCGTTGACCATGCGGTGAGAAc                                                                 <  1:615742/150‑1 (MQ=255)
                                                                                      gcgaATCAGACGTGCAAAGAGTCCCGTTATGCCGCAGAGGACTATTAGCGCGTTGGTGACAATAAAAACGCCGATGGCTTCGTTAAGTGTTAGTCCCTGCAATCCGGTGACCAACAAAGCCGCGCCAGGCGTTGACCATGCGGTGAGAAc                                                                 <  2:871375/150‑1 (MQ=255)
                                                                                        gcATCAGACGAGCAAAGTGTCCCGTTATGCCGCAGAGGAATATTAGCGCGTTGGTGACAATAAAAACGCCGATGGCTTCGTTAAGTGTTAGTCCCTGCAATCCGGTGACCAACAAAGCCGCGCCAGGCGTTGACCATGCGGTGAGAACAg                                                               <  1:4304125/150‑1 (MQ=255)
                                                                                        gcATCAGACGAGCAAAGAGTCCCGTTATGCCGCAGAGGACTATTAGCGCGTTGGTGACAATAAAAACGCCGATGGCTTCGTTAAGTGTTAGTCCCTGCAATCCGGTGACCAACAAAGCCGCGCCAGGCGTTGACCATGCGGTGAGAACAg                                                               >  1:2760742/1‑150 (MQ=255)
                                                                                         catcatACGAGCAAAGAGTCCCGTTATGCCGCAGAGGACTATTAGCGCGTTGGTGACAATAAAAACGCCGATGGCTTCGTTAAGTGTTAGTCCCTGCAATCCGGTGACCAACAAAGCCGCGCCAGGCGTTGACCATGCGGTGAGAACAgg                                                              <  2:1534620/150‑1 (MQ=255)
                                                                                         cATCAGCCGAGCAACGCGTCCCGTTAGGCCGCAGAGGAATTTTAGCGCGTTGGTGACAATAAAAACGCCGATGGCTTCGTTAAGTGTTAGTCCCTGCAATCCGGTGACCAACAAAGCCGCGCCAGGCGTTGACCATGCGGTGAGAACAgg                                                              <  2:2319569/150‑1 (MQ=255)
                                                                                         cATCAGACGAGCAAAGAGTCCCGTTTTGCCGCAGTGGACTTTTAGCGGGTGGGTTAAAATAAAAACGCCGATGGCTTCGTTAAGTGTTAGTCCCTGCAATCCGGTGACCAACAAAGCCGCGCCAGGCGTTGACCATGCGGTGAGAACAgg                                                              <  1:2468946/150‑1 (MQ=255)
                                                                                          aTCAGACGAGCAAAGAGTCCCGTTATGCCGCAGAGGACTATTAGCGCGTTGGTGACAATAAAAACGCCGATGGCTTCGTTAAGTGTTAGTCCCTGCAATCCGGTGACCAACAAAGCCGCGCCAGGCGTTGACCATGCGGTGAGAACAGGt                                                             >  2:3416313/1‑150 (MQ=255)
                                                                                          aTCAGACGAGCAAAGAGTCCCGTTATGCCGCAGAGGACTATTAGCGCGTTGGTGACAATAAAAACGCCGATGGCTTCGTTAAGTGTTAGTCCCTGCAATCCGGTGACCAACAAAGCCGCGCCAGGCGTTGACCATGCGGTGAGAACAGGt                                                             >  1:3031667/1‑150 (MQ=255)
                                                                                          aTCAGACGAGCAAAGAGTCCCGTTATGCCGCAGAGGACTATTAGCGCGTTGGTGACAATAAAAACGCCGATGGCTTCGTTAAGTGTTAGTCCCTGCAATCCGGTGACCAACAAAGCCGCGCCAGGCGTTGACCATGCGGTGAGAACAGGt                                                             >  2:4601461/1‑150 (MQ=255)
                                                                                          aTCAGACGAGAAACGTGACCCTTTAGGCCGCTGCGGACTTTTCGCGCGTTGGTGACAATAAACACGCCGATGGCTTCGTTAAGTGTTAGTCCCTGCAATCCGGTGACCAACAAAGCCGCGCCAGGCGTTGACCATGCGGTGAGAACAGGt                                                             <  1:1480117/150‑1 (MQ=255)
                                                                                             aGACTAGAAAAGAGTCCCTTTTTGCCGCTGTGTAATATTAGCGCTTTGGTTAAAAAAAAAACGCCGATGGCTTCGTTAAGTGTTAGTCCCTGCAATCCGGTGACCAACAAAGCCGCGCCAGGCGTTGACCATGCGGTGAGAACAGGTAcg                                                          <  1:1617888/150‑1 (MQ=255)
                                                                                             aGACGAGCAAAGATTCCCGTTTTGCCGCAGAGGACTATTAGCGGGTTGGTTACAAAAAAAACGCCGATGGCTTCGTTAAGTGTTAGTCCCTGCAATCCGGTGACCAACAAAGCCGCGCCAGGCGTTGACCATGCGGTGAGAACAGGTAcg                                                          <  2:1736463/150‑1 (MQ=255)
                                                                                              gACGAGCAAAGAGTCCCGTTATGCCGCAGAGGACTATTAGCGCGTTGGTGACAATTAAAACGCCGATGGCTTCGTTAAGTGTTAGTCCCTGCAATCCGGTGACCAACAAAGCCGCGCCAGGCGTTGACCATGCGGTGAGAACAGGTAcgc                                                         <  1:3169370/150‑1 (MQ=255)
                                                                                              gACGAGCAAAGAGTCCCGTTATGCCGCAGAGGACTATTAGCGCGTTGGTGACAATAAAAACGCCGATGGCTTCGTTAAGTGTTAGTCCCTGCAATCCGGTGACCAACAAAGCCGCGCCAGGCGTTGACCATGCGGTGAGAACAGGTAcgc                                                         >  2:144331/1‑150 (MQ=255)
                                                                                                 gAGCAAAGAGTCCCGTTATGCCGAAGAGGACTATTAGCGCGTTGGTGACAATAAACACGCCGATGGCTTCGTTAAGTGTTAGTCCCTGCAATCCGGTGACCAACAAAGCCGCGCCAGGCGTTGACCATGCGGTGAGAACAGGTACGCGAt                                                      <  1:3423289/150‑1 (MQ=255)
                                                                                                     aaaGAGTCCCGTTATGCCGCAGAGGACTATTAGCGCGTTGGTGACAATAAAAACGCCGATGGCTTCGTTAAGTGTTAGTCCCTGCAATCCGGTGACCAACAAAGCCGCGCCAGGCGTTGACCATGCGGTGAGAACAGGTACGCGATACCa                                                  >  1:2016978/1‑150 (MQ=255)
                                                                                                      aaGTGTCCCGTTATGCCGCAGAGGACTATTTGCGCGTTGGTGACAATAAAAACGCCGATGGCTTCGTTAAGTGTTAGTCCCTGCAATCCGGTGACCAACAAAGCCGCGCCAGGCGTTGACCATGCGGTGAGAACAGGTACGCGATACCAt                                                 <  1:4240840/150‑1 (MQ=255)
                                                                                                      aaGAGTCCCGTTATGCCGCAGAGGACTATTAGCGCGTTGGTGACAATAAAAACGCCGATGGCTTCGTTAAGTGTTAGTCCCTGCAATCCGGTGACCAACAAAGCCGCGCCAGGCGTTGACCATGCGGTGAGAACAGGTACGCGATACCAt                                                 >  1:3567682/1‑150 (MQ=255)
                                                                                                        gagTCCCTTTATGCCGCTGAGTAATATTCGCGCGTTGGTGACAATAAAAACGCCGATGGCTTCGTTAAGTGTTAGTCCCTGCAATCCGGTGACCAACAAAGCCGCGCCAGGCGTTGACCATGCGGTGAGAACAGGTACGCGATACCATaa                                               <  1:3205893/150‑1 (MQ=255)
                                                                                                         agTCCCGTTATGCCGCAGAGGACTATTAGCGCGTTGGTGACAATAAAAACGCCGATGGCTTCGTTAAGTGTTAGTCCCTGCAATCCGGTGACCAACAAAGCCGCGCCAGGCGTTGACCATGCGGTGAGAACAGGTACGCGATACCATAAt                                              <  2:4459182/150‑1 (MQ=255)
                                                                                                         agTCCCGTTATGCCGCAGAGGACTATTAGCGCGTTGGTGACAATAAAAACGCCGATGGCTTCGTTAAGTGTTAGTCCCTGCAATCCGGTGACCAACAAAGCCGCGCCAGGCGTTGACCATGCGGTGAGAACAGGTACGCGATACCATAAt                                              >  1:4150237/1‑150 (MQ=255)
                                                                                                            cccGTTATGCCGCAGAGGACTATTAGCGCGTTGGTGACAATAAAAACGCCGATGGCTTCGTTAAGTGTTAGTCCCTGCAATCCGGTGACCAACAAAGCCGCGCCAGGCGTTGACCATGCGGTGAGAACAGGTACGCGATACCATAATGTc                                           >  1:4725625/1‑150 (MQ=255)
                                                                                                                ttATGCCGCAGAGGACTATTAGCGCGTTGGTGACAATAAAAACGCCGATGGCTTCGTTAAGTGTTAGTCCCTGCAATCCGGTGACCAACAAAGCCGCGCCAGGCGTTGACCATGCGGTGAGAACAGGTACGCGATACCATAATGTCagag                                       >  1:667929/1‑150 (MQ=255)
                                                                                                                 tATGCCGCAGAGGACTATTAGCGCGTTGGTGACAATAAAAACGCCGATGGCTTCGTTAAGTGTTAGTCCCTGCAATCCGGTGACCAACAAAGCCGCGCCAGGCGTTGACCATGCGGTGAGAACAGGTACGCGATACCATAATGTCAGAGt                                      <  2:2126769/150‑1 (MQ=255)
                                                                                                                  ttGCCGCTGTGGACTTTTAGCTCGTTGTTGACAATAAAAACGCCGATGGCTTCGTTAAGTGTTAGTCCCTGCAATCCGGTGACCAACAAAGCCGCGCCAGGCGTTGACCATGCGGTGAGAACAGGTACGCGATACCATAATGTCAGAGTc                                     <  2:1081758/149‑1 (MQ=255)
                                                                                                                       gCAGAGGACTATTAGCGCGTTGGTGACAATAAAAACGCCGATGGCTTCGTTAAGTGTTAGTCCCTGCAATCCGGTGACCAACAAAGCCGCGCCAGGCGTTGACCATGCGGTGAGAACAGGTACGCGATACCATAATGTCAGAGTCAGCGt                                >  1:3492380/1‑150 (MQ=255)
                                                                                                                        cAGAGGACTATGAGCGCGTTGGTGACAATAAAAACGCCGATGGCTTCGTTAAGTGTTAGTCCCTGCAATCCGGTGACCAACAAAGCCGCGCCAGGCGTTGACCATGCGGTGAGAACAGGTACGCGATACCATAATGTCAGAGTCAGCGTa                               <  2:8175/150‑1 (MQ=255)
                                                                                                                          ttgtaCTTTTAGCGCGTTGGTGACAATAAAAACGCCGATGGCTTCGTTAAGTGTTAGTCCCTGCAATCCGGTGACCAACAAAGCCGCGCCAGGCGTTGACCATGCGGTGAGAACAGGTACGCGATACCATAATGTCAGAGTCAGCGTACt                             <  2:631457/146‑1 (MQ=255)
                                                                                                                            ggACTATTAGCGCGTTGGTGACAATAAAAACGCCGATGGCTTCGTTAAGTGTTAGTCCCTGCAATCCGGTGACCAACAAAGCCGCGCCAGGCGTTGACCATGCGGTGAGAACAGGTACGCGATACCATAATGTCAGAGTCAGCGTACTGa                           >  1:2538323/1‑150 (MQ=255)
                                                                                                                             gACTATTCGCGGGTTGGTGAAAATAAAAACGCCGATGGCTTCGTTAAGTGTTAGTCCCTGCAATCCGGTGACCAACAAAGCCGCGCCAGGCGTTGACCATGCGGTGAGAACAGGTACGCGATACCATAATGTCAGAGTCAGCGTACTGAc                          <  1:2626693/150‑1 (MQ=255)
                                                                                                                             gACTATTAGCGCGTTGGTGAAAATAAAAACGCCGATGGCTTCGTTAAGTGTTAGTCCCTGCAATCCGGTGACCAACAAAGCCGCGCCAGGCGTTGACCATGCGGTGAGAACAGGTACGCGATACCATAATGTCAGAGTCAGCGTACTGAc                          <  1:1581805/150‑1 (MQ=255)
                                                                                                                              aCTATTAGCGCGTTGGTGACAATAAAAACGCCGATGGCTTCGTTAAGTGTTAGTCCCTGCAATCCGGTGACCAACAAAGCCGCGCCAGGCGTTGACCATGCGGTGAGAACAGGTACGCGATACCATAATGTCAGAGTCAGCGTACTGACg                         >  1:2295394/1‑150 (MQ=255)
                                                                                                                               cTATTAGCGCGTTGGTGACAATAAAAACGCCGATGGCTTCGTTAAGTGTTAGTCCCTGCAATCCGGTGACCAACAAAGCCGCGCCAGGCGTTGACCATGCGGTGAGAACAGGTACGCGATACCATAATGTCAGAGTCAGCGTACTGACGc                        >  2:1969944/1‑150 (MQ=255)
                                                                                                                                 aTTAGCGCGTTGGTGACAATAAAAACGCCGATGGCTTCGTTAAGTGTTAGTCCCTGCAATCCGGTGACCAACAAAGCCGCGCCAGGCGTTGACCATGCGGTGAGAACAGGTACGCGATACCATAATGTCAGAGTCAGCGTACTGACGccc                      >  2:3218452/1‑150 (MQ=255)
                                                                                                                                 aTTAGCGCGTTGGTGACAATAAAAACGCCGATGGCTTCGTTAAGTGTTAGTCCCTGCAATCCGGTGACCAACAAAGCCGCGCCAGGCGTTGACCATGCGGTGAGAACAGGTACGCGATACCATAATGTCAGAGTCAGCGTACTGACGccc                      >  2:1148990/1‑150 (MQ=255)
                                                                                                                                  tttgcgcGTTGTTCACAATAAACACGCCGATGGCTTCTTTAAGTTTTAGTCCCTGCAATCCGGTGAACAACTAAGCCGCGCCAGGCGTTGACCATGCGGTGAGATCAGGTAAGCGATACCATAATGTCAGAGTCAGCGTACTGACGCCCa                     <  1:2031658/147‑1 (MQ=255)
                                                                                                                                    cgcgcGTTGGTGACAAAAAAAACGCCGATGGCTTCGTTAAGTGTTAGTCCCTGCAATCCGGTGACCAACAAAGCCGCGCCAGGCGTTGACCATGCGGTGAGAACAGGTACGCGATACCATAATGTCAGAGTCAGCGTACTGACGCCCAtt                   <  1:1909876/149‑1 (MQ=255)
                                                                                                                                    aGCGCGTTGGTGACAATAAAAACGCCGATGGCTTCGTTAAGTGTTAGTCCCTGCAATCCGGTGACCAACAAAGCCGCGCCAGGCGTTGACCATGCGGTGAGAACAGGTACGCGATACCATAATGTCAGAGTCAGCGTACTGACGCCCAtt                   >  1:4701975/1‑150 (MQ=255)
                                                                                                                                     gcgcGTTGGTGACAATAAAAACGCCGATGGCTTCGTTAAGTGTTAGTCCCTGCAATCCGGTGACCAACAAAGCCGCGCCAGGCGTTGACCATGCGGTGAGAACAGGTACGCGATACCATAATGTCAGAGTCAGCGTACTGACGCCCATTg                  >  2:53647/1‑150 (MQ=255)
                                                                                                                                       gcttgggTGACAAAAAACACGCCGATGGCTTCGTTAAGTGTTAGTCCCTGCAATCCGGTGACCAACAAAGCCGCGCCAGGCGTTGACCATGCGGTGAGAACAGGTACGCGATACCATAATGTCAGAGTCAGCGTACTGACGCCCATTGcc                <  1:1170397/145‑1 (MQ=255)
                                                                                                                                          ttGGTGAAAATAAAAACGCCGATGGCTTCGTTAAGTGTTAGTCCCTGCAATCCGGTGACCAACAAAGCCGCGCCAGGCGTTGACCATGCGGTGAGAACAGGTACGCGATACCATAATGTCAGAGTCAGCGTACTGACGCCCATTGCCAGc             <  1:585162/150‑1 (MQ=255)
                                                                                                                                           tgtttacaaaacaaaCGCCGATGGCTTCGTTAAGTGTTAGTCCCTGCAATCCGGTGACCAACAAAGCCGCGCCAGGCGTTGACCATGCGGTGAGAACAGGTACGCGATACCATAATGTCAGAGTCAGCGTACTGACGCCCATTGCCAGcc            <  1:1083127/145‑1 (MQ=255)
                                                                                                                                            ggTGACAATAAAAACGCCGATGGCTTCGTTAAGTGTTAGTCCCTGCAATCCGGTGACCAACAAAGCCGCGCCAGGCGTTGACCATGCGGTGAGAACAGGTACGCGATACCATAATGTCAGAGTCAGCGTACTGACGCCCATTGCCAGccc           >  2:4560702/1‑150 (MQ=255)
                                                                                                                                             gTGACAATAAAAACGCCGATGGCTTCGTTAAGTGTTAGTCCCTGCAATCCGGTGACCAACAAAGCCGCGCCAGGCGTTGACCATGCGGTGAGAACAGGTACGCGATACCATAATGTCAGAGTCAGCGTACTGACGCCCATTGCCAGcccc          >  1:749636/1‑150 (MQ=255)
                                                                                                                                             gTGACAATAAAAACGCCGATGGCTTCGTTAAGTGTTAGTCCCTGCAATCCGGTGACCAACAAAGCCGCGCCAGGCGTTGACCATGCGGTGAGAACAGGTACGCGATACCATAATGTCAGAGTCAGCGTACTGACGCCCATTGCCAGcccc          >  1:271621/1‑150 (MQ=255)
                                                                                                                                                  aaTAAAAACGCCGATGGCTTCGTTAAGTGTTAGTCCCTGCAATCCGGTGACCAACAAAGCCGCGCCAGGCGTTGACCATGCGGTGAGAACAGGTACGCGATACCATAATGTCAGAGTCAGCGTACTGACGCCCATTGCCAGCCCCAgcgc     >  1:3295653/1‑150 (MQ=255)
                                                                                                                                                  aaTAAAAACGCCGATGGCTTCGTTAAGTGTTAGTCCCTGCAATCCGGTGACCAACAAAGCCGCGCCAGGCGTTGACCATGCGGTGAGAACAGGTACGCGATACCATAATGTCAGAGTCAGCGTACTGACGCCCATTGCCAGCCCCAgcgc     >  1:3295641/1‑150 (MQ=255)
                                                                                                                                                   aTAAAAACGCCGATGGCTTCGTTAAGTGTTAGTCCCTGCAATCCGGTGACCAACAAAGCCGCGCCAGGCGTTGACCATGCGGTGAGAACAGGTACGCGATACCATAATGTCAGAGTCAGCGTACTGACGCCCATTGCCAGCCCCAGCGcc    >  2:1401552/1‑150 (MQ=255)
                                                                                                                                                   aTAAAAACGCCGATGGCTTCGTTAAGTGTTAGTCCCTGCAATCCGGTGACCAACAAAGCCGCGCCAGGCGTTGACCATGCGGTGAGAACAGGTACGCGATACCATAATGTCAGAGTCAGCGTACTGACGCCCATTGCCAGCCCCAGCGcc    <  2:2814956/150‑1 (MQ=255)
                                                                                                                                                     aaaaaCGCCGATGGCTTCGTTAAGTGTTAGTCCCTGCAATCCGGTGACCAACAAAGCCGCGCCAGGCGTTGACCATGCGGTGAGAACAGGTACGCGATACCATAATGTCAGAGTCAGCGTACTGACGCCCATTGCCAGCCCCAGCGCCGt  >  2:1682192/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TGACCGTCCAGACTGGCAAACGCCTGTAAACCAAAGCGTAATAAAATCCCGGCAAGCATTGCCGCCGCAAGCGAGTGCGGAATAATGCGCATCAGACGAGCAAAGAGTCCCGTTATGCCGCAGAGGACTATTAGCGCGTTGGTGACAATAAAAACGCCGATGGCTTCGTTAAGTGTTAGTCCCTGCAATCCGGTGACCAACAAAGCCGCGCCAGGCGTTGACCATGCGGTGAGAACAGGTACGCGATACCATAATGTCAGAGTCAGCGTACTGACGCCCATTGCCAGCCCCAGCGCCGT  >  CP009273/1499898‑1500196

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑