Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,711,397 C→T 95.0% R28Q (CGA→CAA)  tyrS ← tyrosyl‑tRNA synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,711,3970CT95.0% 851.8 / 21.4 257R28Q (CGA→CAA) tyrStyrosyl‑tRNA synthetase
Reads supporting (aligned to +/- strand):  ref base C (5/8);  new base T (104/140);  total (109/148)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.33e-01

Only 100 of 257 total aligned reads displayed.

CCGTCGCGCCGCCTACCAGCGCAACCGGCTTGTGGCCCGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTCGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCACCTTTGCCGGTACACGACTTTTCGCCAGATGCGAAA  >  CP009273/1711251‑1711543
                                                                                                                                                  |                                                                                                                                                  
ccGTCGCGCCGCCTACCAGCGCAACCGGCTTGTGGCCCGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGct                                                                                                                                                 >  1:3854137/1‑150 (MQ=255)
 cGTCGCGCCGCCTACCAGCGCAACCGGCTTGTGGCCCGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTCGctc                                                                                                                                                <  1:2491860/150‑1 (MQ=255)
 cGTCGCGCCGCCTACCAGCGCAACCGGCTTGTGGCCCGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTCGctc                                                                                                                                                <  1:5071144/150‑1 (MQ=255)
     gcgcCGCCTACCAGCGCAACCGGCTTGTGGCCCGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCt                                                                                                                                            >  1:1679868/1‑150 (MQ=255)
       gccgccTACCAGCGCAACCGGCTTGTGGCCCGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGGTGCTCTGCTaa                                                                                                                                          >  1:355821/1‑150 (MQ=255)
        ccgccTACCAGCGCAACCGGCTTGTGGCCCGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAAc                                                                                                                                         <  2:1307025/150‑1 (MQ=255)
        ccgccTACCAGCGCAACCGGCTTGTGGCCCGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAAc                                                                                                                                         <  1:1568586/150‑1 (MQ=255)
         cgccTACCAGCGCAACCGGCTTGTGGCCCGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACg                                                                                                                                        <  1:86209/150‑1 (MQ=255)
          gccTACCAGCGCAACCGGCTTGTGGCCCGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGc                                                                                                                                       <  2:3386422/150‑1 (MQ=255)
            cTACCAGCGCAACCGGCTTGTGGCCCGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCtt                                                                                                                                     >  2:335395/1‑150 (MQ=255)
            cTACCAGCGCAACCGGCTTGTGGCCCGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCtt                                                                                                                                     >  2:1371601/1‑150 (MQ=255)
                    gcAACCGGCTTGTGGCCCGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTcgtcc                                                                                                                             <  2:1049028/150‑1 (MQ=255)
                     cAACCGGCTTGTGGCCCGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTcgtccg                                                                                                                            >  2:2079285/1‑150 (MQ=255)
                       aCCGGCTTGTGGCCCGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTcgtccgtc                                                                                                                          <  2:5060106/150‑1 (MQ=255)
                       aCCGGCTTGTGGCCCGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTCGCTCTGCTAACGCTTCCTcgtccgtc                                                                                                                          <  1:2898704/150‑1 (MQ=255)
                          ggCTTGTGGCCCGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCAcc                                                                                                                       <  2:2972471/150‑1 (MQ=255)
                           gCTTGTGGCCCGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCt                                                                                                                      <  1:3681496/150‑1 (MQ=255)
                              tgtgGCCCGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTggg                                                                                                                   <  1:910921/150‑1 (MQ=255)
                                tgGCCCGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCt                                                                                                                 <  1:2835626/150‑1 (MQ=255)
                                 ggCCCGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTa                                                                                                                >  2:4547136/1‑150 (MQ=255)
                                   cccGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTAcc                                                                                                              >  1:4390523/1‑150 (MQ=255)
                                    ccGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTCGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCa                                                                                                             <  1:1064678/150‑1 (MQ=255)
                                     cGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAg                                                                                                            <  2:1906434/150‑1 (MQ=255)
                                        ctgctgGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGccc                                                                                                         >  2:3838750/1‑150 (MQ=255)
                                         tgctgGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGTCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGcccc                                                                                                        >  2:544650/1‑150 (MQ=255)
                                            tgGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGc                                                                                                     >  2:286827/1‑150 (MQ=255)
                                             ggAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGct                                                                                                    >  1:4841710/1‑150 (MQ=255)
                                               aaGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGctct                                                                                                  <  1:2711336/150‑1 (MQ=255)
                                                aGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCtt                                                                                                 >  2:4966908/1‑150 (MQ=255)
                                                aGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTCGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCtt                                                                                                 <  2:3974558/150‑1 (MQ=255)
                                                   gTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCa                                                                                              >  1:4579471/1‑150 (MQ=255)
                                                     ttCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAAt                                                                                            <  2:2379254/150‑1 (MQ=255)
                                                     ttCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAAt                                                                                            >  1:3808022/1‑150 (MQ=255)
                                                     ttCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAAt                                                                                            <  1:2614388/150‑1 (MQ=255)
                                                       cAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGGTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTg                                                                                          <  1:191246/150‑1 (MQ=255)
                                                         ggCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGtt                                                                                        <  1:3151183/150‑1 (MQ=255)
                                                            aTAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTCGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTaa                                                                                     >  1:2731229/1‑150 (MQ=255)
                                                              aaCAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATc                                                                                   >  1:2291470/1‑150 (MQ=255)
                                                              aaCAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATc                                                                                   <  2:425462/150‑1 (MQ=255)
                                                               aCAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCa                                                                                  >  1:1091839/1‑150 (MQ=255)
                                                               aCAATGGAACAAGATGCCCCAAAGGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCa                                                                                  <  1:3127587/150‑1 (MQ=255)
                                                                 aaTGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAg                                                                                >  1:3306654/1‑150 (MQ=255)
                                                                  aTGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGt                                                                               >  2:2281336/1‑150 (MQ=255)
                                                                    ggAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTa                                                                             <  2:1364382/150‑1 (MQ=255)
                                                                      aaCAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACt                                                                           >  2:1621519/1‑150 (MQ=255)
                                                                      aaCAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACt                                                                           >  1:578823/1‑150 (MQ=255)
                                                                      aaCAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACt                                                                           >  2:3320897/1‑150 (MQ=255)
                                                                          aGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCtt                                                                       <  2:1161365/150‑1 (MQ=255)
                                                                           gATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTg                                                                      >  1:1398238/1‑150 (MQ=255)
                                                                              gCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCa                                                                   >  2:1725183/1‑150 (MQ=255)
                                                                               ccccAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCAt                                                                  <  1:3076682/150‑1 (MQ=255)
                                                                                 ccAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCa                                                                >  2:134613/1‑150 (MQ=255)
                                                                                    aaTGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCaaaa                                                             >  2:3680002/1‑150 (MQ=255)
                                                                                    aaTGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCaaaa                                                             <  2:2963454/150‑1 (MQ=255)
                                                                                     aTGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAAt                                                            <  2:3262205/150‑1 (MQ=255)
                                                                                      tGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAAtc                                                           <  1:3599959/150‑1 (MQ=255)
                                                                                       gCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAAtct                                                          <  2:4682643/150‑1 (MQ=255)
                                                                                       gCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAAtct                                                          <  2:4682654/150‑1 (MQ=255)
                                                                                       gCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAAtct                                                          >  2:4797541/1‑150 (MQ=255)
                                                                                            cTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATg                                                     <  2:2517904/150‑1 (MQ=255)
                                                                                              gTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGta                                                   >  1:3930408/1‑150 (MQ=255)
                                                                                              gTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGta                                                   >  2:761871/1‑150 (MQ=255)
                                                                                               tCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGtat                                                  <  1:2046679/150‑1 (MQ=255)
                                                                                               tCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGtat                                                  <  2:4958797/150‑1 (MQ=255)
                                                                                                  gCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGtatata                                               <  1:1775727/150‑1 (MQ=255)
                                                                                                    ggTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAAc                                             >  2:4929082/1‑150 (MQ=255)
                                                                                                     gTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACg                                            >  2:402574/1‑150 (MQ=255)
                                                                                                       aGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGAc                                          >  2:2023403/1‑150 (MQ=255)
                                                                                                        ggATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACt                                         <  1:488815/150‑1 (MQ=255)
                                                                                                        ggATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACt                                         <  2:1399621/150‑1 (MQ=255)
                                                                                                         gATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTg                                        <  2:4475497/150‑1 (MQ=255)
                                                                                                          aTCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGc                                       <  2:5146981/150‑1 (MQ=255)
                                                                                                          aTCGAAGCCGAAATAGCGCGCGATCGGGCCTTGCGCCTGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGATACCAGCCCCCGATCTTGAATTTGTTTAATAAATTTAGTGATTGCCATAAAAATCTCCTTTTATATAACGACTGc                                       <  1:1369149/150‑1 (MQ=255)
                                                                                                           tCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTCGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCa                                      >  1:2423134/1‑150 (MQ=255)
                                                                                                            cGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCAc                                     >  2:301936/1‑150 (MQ=255)
                                                                                                               aGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCACCtt                                  <  2:4383553/150‑1 (MQ=255)
                                                                                                               aGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGAAATTGTTTAATCATGTTACTGCTTGCCATCAAAATCTCCTTGTATATAACGACTGCACCtt                                  <  1:335395/150‑1 (MQ=255)
                                                                                                                gCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCACCttt                                 >  1:620656/1‑150 (MQ=255)
                                                                                                                gCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCACCttt                                 <  2:355821/150‑1 (MQ=255)
                                                                                                                 ccGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTATTGAAATTGTTTAATCAATTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCACCTTTg                                <  1:1240541/150‑1 (MQ=255)
                                                                                                                  cGCAATAGTGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCACCTTTGc                               <  2:1754628/150‑1 (MQ=255)
                                                                                                                        agagCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCACCTTTGCCGGTac                         <  2:3605401/150‑1 (MQ=255)
                                                                                                                         gagCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCACCTTTGCCGGTcca                        >  1:605424/1‑150 (MQ=255)
                                                                                                                          agCGCGATCGGGCCTTGCGCCTGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAATTTACGGCTTGCCATCAAAATCTCCATGTATATAACGACTGCACCTTTGCCGGTacac                       <  1:3536737/150‑1 (MQ=255)
                                                                                                                               gATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCACCTTTGCCGGTACACGACtt                  >  1:4625910/1‑150 (MQ=255)
                                                                                                                                  cGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCACCTTTGCCGGTACACGACTTTTc               >  1:4132812/1‑150 (MQ=255)
                                                                                                                                  cGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCACCTTTGCCGGTACACGACTTTTc               <  1:4706168/150‑1 (MQ=255)
                                                                                                                                     gCCTTGCGCCAGGTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAAGTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCACCTTTGCCGGTACACGACTTTTCGcc            <  2:164208/150‑1 (MQ=255)
                                                                                                                                       cTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCACCTTTGCCGGTACACGACTTTTCGCCAg          >  1:3181814/1‑150 (MQ=255)
                                                                                                                                       cTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCACCTTTGCCGGTACACGACTTTTCGCCAg          >  1:4977226/1‑150 (MQ=255)
                                                                                                                                        ttGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCACCTTTGCCGGTACACGACTTTTCGCCAGa         >  1:3282347/1‑150 (MQ=255)
                                                                                                                                         tGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCACCTTTGCCGGTACACGACTTTTCGCCAGAt        <  2:1231923/150‑1 (MQ=255)
                                                                                                                                         tGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCACCTTTGCCGGTACACGACTTTTCGCCAGAt        >  1:3840253/1‑150 (MQ=255)
                                                                                                                                         tGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCACCTTTGCCGGTACACGACTTTTCGCCAGAt        <  2:3654150/150‑1 (MQ=255)
                                                                                                                                          gcgcCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCACCTTTGCCGGTACACGACTTTTCGCCAGATg       >  2:1868875/1‑150 (MQ=255)
                                                                                                                                            gcCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTCTATAACGACTGCACCGTTGCCGGTACACGACTTTTCGCCAGATGCg     >  1:561698/1‑150 (MQ=255)
                                                                                                                                            gcCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCACCTTTGCCGGTACACGACTTTTCGCCAGATGCg     >  1:4243690/1‑150 (MQ=255)
                                                                                                                                            gcCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCACCTTTGCCGGTACACGACTTTTCGCCAGATGCg     >  2:2608537/1‑150 (MQ=255)
                                                                                                                                             ccAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTTTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCACCTTTGCCGGTACACGACTTTTCGCCAGATGCGa    <  1:3937338/150‑1 (MQ=255)
                                                                                                                                             ccAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCACCTTTGCCGGTACACGACTTTTCGCCAGATGCGa    <  2:2670359/150‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
CCGTCGCGCCGCCTACCAGCGCAACCGGCTTGTGGCCCGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTCGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCACCTTTGCCGGTACACGACTTTTCGCCAGATGCGAAA  >  CP009273/1711251‑1711543

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑