Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,872,520 G→T 90.4% P183P (CCG→CCT)  yeaP → diguanylate cyclase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,872,5200GT90.4% 831.3 / 68.0 271P183P (CCG→CCT) yeaPdiguanylate cyclase
Reads supporting (aligned to +/- strand):  ref base G (12/14);  new base T (123/122);  total (135/136)
Fisher's exact test for biased strand distribution p-value = 8.37e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01

Only 100 of 271 total aligned reads displayed.

GTGGAGTGAACGCGCGGAACAGGTTTTACAGTTATTCGCCGGACTGATTGCACAATATATTCAAAAAGAGGCACTGGTTGAACAGCTGCGCGAAGCCAATGCTGCGCTGATTGCGCAATCGTATACCGACTCGTTAACCGGGCTACCGAATCGGCGGGCGATTTTTGAAAATCTGACGACACTGTTTTCCCTCGCCCGGCATCTTAACCATAAGATAATGATCGCGTTTATCGATCTGGATAACTTCAAATTAATCAATGATCGTTTTGGTCATAATAGTGGCGATCTGTTTCT  >  CP009273/1872373‑1872666
                                                                                                                                                   |                                                                                                                                                  
      tGAACGCGCGGAACAGGTTTTACAGTTATTCGCCGGACTGATTGCACAATATATTCAAAAAGAGGCACTGGTTGAACAGCTGCGCGAAGCCAATGCTGCGCTGATTGCGCAATCGTATACCGACTCGTTAACCGGGCTACCTAATcggcg                                                                                                                                            >  1:1752891/1‑150 (MQ=255)
       gAACGCGCGGAACAGGTTTTACAGTTATTCGCCGGACTGATTGCACAATATATTCAAAAAGAGGCACTGGTTGAACAGCTGCGCGAAGCCAATGCTGCGCTGATTGCGCAATCGTATACCGACTCGTTAACCGGGCTACCTAATcggcgg                                                                                                                                           <  2:4167307/150‑1 (MQ=255)
        aaCGCGCGGAACAGGTTTTACAGTTATTCGCCGGACTGATTGCACAATATATTCAAAAAGAGGCACTGGTTGAACAGCTGCGCGAAGCCAATGCTGCGCTGATTGCGCAATCGTATACCGACTCGTTAACCGGGCTACCTAATCggcggg                                                                                                                                          >  1:2767171/1‑150 (MQ=255)
        aaCGCGCGGAACAGGTTTTACAGTTATTCGCCGGACTGATTGCACAATATATTCAAAAAGAGGCACTGGTTGAACAGCTGCGCGAAGCCAATGCTGCGCTGATTGCGCAATCGTATACCGACTCGTTAACCGGGCTACCTAATCggcggg                                                                                                                                          >  1:2484031/1‑150 (MQ=255)
           gcgcgGAACAGGTTTTACAGTTATTCGCCGGACTGATTGCACAATATATTCAAAAAGAGGCACTGGTTGAACAGCTGCGCGAAGCCAATGCTGCGCTGATTGCGCAATCGTATACCGACTCGTTAACCGGGCTACCTAATCGGCGGGCGa                                                                                                                                       <  1:762011/150‑1 (MQ=255)
            cgcgGAACAGGTTTTACAGTTATTCGCCGGACTGATTGCACAATATATTCAAAAAGAGGCACTGGTTGAACAGCTGCGCGAAGCCAATGCTGCGCTGATTGCGCAATCGTATACCGACTCGTTAACCGGGCTACCTAATCGGCGGGCGAt                                                                                                                                      >  1:2665192/1‑150 (MQ=255)
              cgGAACAGGTTTTACAGTTATTCGCCGGACTGATTGCACAATATATTCAAAAAGAGGCACTGGTTGAACAGCTGCGCGAAGCCAATGCTGCGCTGATTGCGCAATCGTATACCGACTCGTTAACCGGGCTACCTAATCGGCGGGCGAttt                                                                                                                                    <  1:3365931/150‑1 (MQ=255)
                 aaCAGGTTTTACAGTTATTCGCCGGACTGATTGCACAATATATTCAAAAAGAGGCACTGGTTGAACAGCTGCGCGAAGCCAATGCTGCGCTGATTGCGCAATCGTATACCGACTCGTTAACCGGGCTACCTAATCGGCGGGCGATTTTTg                                                                                                                                 >  2:2001887/1‑150 (MQ=255)
                  aCAGGTTTTACAGTTATTCGCCGGACTGATTGCACAATATATTCAAAAAGAGGCACTGGTTGAACAGCTGCGCGAAGCCAATGCTGCGCTGATTGCGCAATCGTATACCGACTCGTTAACCGGGCTACCTAATCGGCGGGCGATTTTTGa                                                                                                                                <  1:3379505/150‑1 (MQ=255)
                  aCAGGTTTTACAGTTATTCGCCGGACTGATTGCACAATATATTCAAAAAGAGGCACTGGTTGAACAGCTGCGCGAAGCCAATGCTGCGCTGATTGCGCAATCGTATACCGACTCGTTAACCGGGCTACCTAATCGGCGGGCGATTTTTGa                                                                                                                                <  2:5047270/150‑1 (MQ=255)
                   cAGGTTTTACAGTTATTCGCCGGACTGATTGCACAATATATTCAAAAAGAGGCACTGGTTGAACAGCTGCGCGAAGCCAATGCTGCGCTGATTGCGCAATCGTATACCGACTCGTTAACCGGGCTCCCTAATCGGCGGGCGATTTTTGaa                                                                                                                               >  2:1447545/1‑150 (MQ=255)
                   cAGGTTTTACAGTTATTCGCCGGACTGATTGCACAATATATTCAAAAAGAGGCACTGGTTGAACAGCTGCGCGAAGCCAATGCTGCGCTGATTGCGCAATCGTATACCGACTCGTTAACCGGGCTACCTAATCGGCGGGCGATTTTTGaa                                                                                                                               <  1:3894596/150‑1 (MQ=255)
                   cAGGTTTTACAGTTATTCGCCGGACTGATTGCACAATATATTCAAAAAGAGGCACTGGTTGAACAGCTGCGCGAAGCCAATGCTGCGCTGATTGCGCAATCGTATACCGACTCGTTAACCGGGCTACCGAATCGGCGGGCGATTTTTGaa                                                                                                                               >  1:215604/1‑150 (MQ=255)
                     ggTTTTACAGTTATTCGCCGGACTGATTGCACAATATATTCAAAATGAGGCACTGGTTGAACAGCTGCGCGAAGCCAATGCTGCGCTGATTGCGCAATCGTATACCGACTCGTTAACCGGGCTACCTAATCGGCGGGCGATTTTTGaaaa                                                                                                                             >  2:4998010/1‑150 (MQ=255)
                         ttACAGTTATTCGCCGGACTGATTGCACAATATATTCAAAAAGAGGCACTGGTTGAACAGCTGCGCGAAGCCAATGCTGCGCTGATTGCGCAATCGTATACCGACTCGTTAACCGGGCTACCTAATCGGCGGGCGATTTTTGAAAATCTg                                                                                                                         >  2:4357867/1‑150 (MQ=255)
                           aCAGTTATTCGCCGGACTGATTGCACAATATATTCAAAAAGAGGCACTGGTTGAACAGCTGCGCGAAGCCAATGCTGCGCTGATTGCGCAATCGTATACCGACTCGTTAACCGGGCTACCTAATCGGCGGGCGATTTTTGAAAATCTgac                                                                                                                       <  2:3038155/150‑1 (MQ=255)
                              gTTATTCGCCGGACTGATTGCACAATATATTCAAAAAGAGGCACTGGTTGAACAGCTGCGCGAAGCCAATGCTGCGCTGATTGCGCAATCGTATACCGACTCGTTAACCGGGCTACCTAATCGGCGGGCGATTTTTGAAAATCTgacgac                                                                                                                    >  1:1744027/1‑150 (MQ=255)
                                tATTCGCCGGACTGATTGCACAATATATTCAAAAAGAGGCACTGGTTGAACAGCTGCGCGAAGCCAATGCTGCGCTGATTGCGCAATCGTATACCGACTCGTTAACCGGGCTACCTAATCGGCGGGCGATTTTTGAAAATCTGACGacac                                                                                                                  >  1:4468904/1‑150 (MQ=255)
                                tATTCGCCGGACTGATTGCACAATATATTCAAAAAGAGGCACTGGTTGAACAGCTGCGCGAAGCCAATGCTGCGCTGATTGCGCAATCGTATACCGACTCGTTAACCGGGCTACCTAATCGGCGGGCGATTTTTGAAAATCTGACGacac                                                                                                                  <  1:3839570/150‑1 (MQ=255)
                                   tCGCCGGACTGATTGCACAATATATTCAAAAAGAGGCACTGGTTGAACAGCTGCGCGAAGCCAATGCTGCGCTGATTGCGCAATCGTATACCGACTCGTTAACCGGGCTACCTAATCGGCGGGCGATTTTTGAAAATCTGACGACACTGt                                                                                                               >  2:5176004/1‑150 (MQ=255)
                                   tCGCCGGACTGATTGCACAATATATTCAAAAAGAGGCACTGGTTGAACAGCTGCGCGAAGCCAATGCTGCGCTGATTGCGCAATCGTATACCGACTCGTTAACCGGGCTACCTAATCGGCGGGCGATTTTTGAAAATCTGACGACACTGt                                                                                                               >  1:4774628/1‑150 (MQ=255)
                                   tCGCCGGACTGATTGCACAATATATTCAAAAAGAGGCACTGGTTGAACAGCTGCGCGAAGCCAATGCTGCGCTGATTGCGCAATCGTATACCGACTCGTTAACCGGGCTACCTAATCGGCGGGCGATTTTTGAAAATCTGACGACACTGt                                                                                                               <  2:2054112/150‑1 (MQ=255)
                                   tCGCCGGACTGATTGCACAATATATTCAAAAAGAGGCACTGGTTGAACAGCTGCGCGAAGCCAATGCTGCGCTGATTGCGCAATCGTATACCGACTCGTTAACCGGGCTACCTAATCGGCGGGCGATTTTTGAAAATCTGACGACACTGt                                                                                                               <  2:3869232/150‑1 (MQ=255)
                                    cGCCGGACTGATTGCACAATATATTCAAAAAGAGGCACTGGTTGAACAGCTGCGCGAAGCCAATGCTGCGCTGATTGCGCAATCGTATACCGACTCGTTAACCGGGCTACCTAATCGGCGGGCGATTTTTGAAAATCTGACGACACTGtt                                                                                                              <  1:1680173/150‑1 (MQ=255)
                                     gCCGGACTGATTGCACAATATATTCAAAAAGAGGCACTGGTTGAACAGCTGCGCGAAGCCAATGCTGCGCTGATTGCGCAATCGTATACCGACTCGTTAACCGGGCTACCGAATCGGCGGGCGATTTTTGAAAATCTGACGACACTGttt                                                                                                             <  1:1976119/150‑1 (MQ=255)
                                        ggACTGATTGCACAATATATTCAAAAAGAGGCACTGGTTGAACAGCTGCGCGAAGCCAATGCTGCGCTGATTGCGCAATCGTATACCGACTCGTTAACCGGGCTACCTAATCGGCGGGCGATTTTTGAAAATCTGACGACACTGTTTTcc                                                                                                          <  2:4776341/150‑1 (MQ=255)
                                         gACTGATTGCACAATATATTCAAAAAGAGGCACTGGTTGAACAGCTGCGCGAAGCCAATGCTGCGCTGATTGCGCAATCGTATACCGACTCGTTAACCGGGCTACCTAATCGGCGGGCGATTTTTGAAAATCTGACGACACTGTTTTccc                                                                                                         >  2:1611087/1‑150 (MQ=255)
                                          aCTGATTGCACAATATATTCAAAAAGAGGCACTGGTTGAACAGCTGCGCGAAGCCAATGCTGCGCTGATTGCGCAATCGTATACCGACTCGTTAACCGGGCTACCTAATCGGCGGGCGATTTTTGAAAATCTGACGACACTGTTTTCCCt                                                                                                        >  2:1410681/1‑150 (MQ=255)
                                            tGATTGCACAATATATTCAAAAAGAGGCACTGGTTGAACAGCTGCGCGAAGCCAATGCTGCGCTGATTGCGCAATCGTATACCGACTCGTTAACCGGGCTACCTAATCGGCGGGCGATTTTTGAAAATCTGACGACACTGTTTTCCCTCg                                                                                                      >  2:4425487/1‑150 (MQ=255)
                                                tGCACAATATATTCAAAAAGAGGCACTGGTTGAACAGCTGCGCGAAGCCAATGCTGCGCTGATTGCGCAATCGTATACCGACTCGTTAACCGGGCTACCTAATCGGCGGGCGATTTTTGAAAATCTGACGACACTGTTTTCCCTCGCCCg                                                                                                  >  2:1547078/1‑150 (MQ=255)
                                                 gCACAATATATTCAAAAAGAGGCACTGGTTGAACAGCTGCGCGAAGCCAATGCTGCGCTGATTGCGCAATCGTATACCGACTCGTTAACCGGGCTACCTAATCGGCGGGCGATTTTTGAAAATCTGACGACACTGTTTTCCCTCGCCCgg                                                                                                 <  2:3999246/150‑1 (MQ=255)
                                                  cacaATATATTCAAAAAGAGGCACTGGTTGAACAGCTGCGCGAAGCCAATGCTGCGCTGATTGCGCAATCGTATACCGACTCGTTAACCGGGCTACCGAATCGGCGGGCGATTTTTGAAAATCTGACGACACTGTTTTCCCTCGCCCGGc                                                                                                >  1:3634943/1‑150 (MQ=255)
                                                     aaTATATTCAAAAAGAGGCACTGGTTGAACAGCTGCGCGAAGCCAATGCTGCGCTGATTGCGCAATCGTATACCGACTCGTTAACCGGGCTACCTAATCGGCGGGCGATTTTTGAAAATCTGACGACACTGTTTTCCCTCGCCCGGCATc                                                                                             >  1:3576947/1‑150 (MQ=255)
                                                      atatatTCAAAAAGAGGCACTGGTTGAACAGCTGCGCGAAGCCAATGCTGCGCTGATTGCGCAATCGTATACCGACTCGTTAACCGGGCTACCTAATCGGCGGGCGATTTTTGAAAATCTGACGACACTGTTTTCCCTCGCCCGGCATCt                                                                                            <  1:3268064/150‑1 (MQ=255)
                                                       tatatTCAAAAAGAGGCACTGGTTGAACAGCTGCGCGAAGCCAATGCTGCGCTGATTGCGCAATCGTATACCGACTCGTTAACCGGGCTACCGAATCGGCGGGCGATTTTTGAAAATCTGACGACACTGTTTTCCCTCGCCCGGCATCtt                                                                                           <  1:4374773/150‑1 (MQ=255)
                                                        atatTCAAAAAGAGGCACTGGTTGAACAGCTGCGCGAAGCCAATGCTGCGCTGATTGCGCAATCGTATACCGACTCGTTAACCGGGCTACCTAATCGGCGGGCGATTTTTGAAAATCTGACGACACTGTTTTCCCTCGCCCGGCATCTTa                                                                                          <  2:1714794/150‑1 (MQ=255)
                                                          atTCAAAAAGAGGCACTGGTTGAACAGCTGCGCGAAGCCAATGCTGCGCTGATTGCGCAATCTTATACCGACTCGTTAACCGGGCTACCGAATCGGCGGGCGATTTTTGAAAATCTGACGACACTGTTTTCCCTCGCCCGGCATCTTAAc                                                                                        <  2:2425423/150‑1 (MQ=255)
                                                          atTCAAAAAGAGGCACTGGTTGAACAGCTGCGCGAAGCCAATGCTGCGCTGATTGCGCAATCGTATACCGACTCGTTAACCGGGCTACCTAATCGGCGGGCGATTTTTGAAAATCTGACGACACTGTTTTCCCTCGCCCGGCATCTTAAc                                                                                        <  2:1303089/150‑1 (MQ=255)
                                                             cAAAAAGAGGCACTGGTTGAACAGCTGCGCGAAGCCAATGCTGCGCTGATTGCGCAATCGTATACCGACTCGTTAACCGGGCTACCTAATCGGCGGGCGATTTTTGAAAATCTGACGACACTGTTTTCCCTCGCCCGGCATCTTAACCAt                                                                                     >  2:3157525/1‑150 (MQ=255)
                                                               aaaaGAGGCACTGGTTGAACAGCTGCGCGAAGCCAATGCTGCGCTGATTGCGCAATCGTATACCGACTCGTTAACCGGGCTACCTAATCGGCGGGCGATTTTTGAAAATCTGACGACACTGTTTTCCCTCGCCCGGCATCTTAACCATaa                                                                                   <  1:4986509/150‑1 (MQ=255)
                                                               aaaaGAGGCACTGGTTGAACAGCTGCGCGAAGCCAATGCTGCGCTGATTGCGCAATCGTATACCGACTCGTTAACCGGGCTACCTAATCGGCGGGCGATTTTTGAAAATCTGACGACACTGTTTTCCCTCGCCCGGCATCTTAACCATaa                                                                                   <  1:2433896/150‑1 (MQ=255)
                                                                aaaGAGGCACTGGTTGAACAGCTGCGCGAAGCCAATGCTGCGCTGATTGCGCAATCGTATACCGACTCGTTAACCGGGCTACCTAATCGGCGGGCGATTTTTGAAAATCTGACGACACTGTTTTCCCTCGCCCGGCATCTTAACCATAAg                                                                                  >  1:3992528/1‑150 (MQ=255)
                                                                aaaGAGGCACTGGTTGAACAGCTGCGCGAAGCCAATGCTGCGCTGATTGCGCAATCGTATACCGACTCGTTAACCGGGCTACCTAATCGGCGGGCGATTTTTGAAAATCTGACGACACTGTTTTCCCTCGCCCGGCATCTTAACCATAAg                                                                                  <  1:283530/150‑1 (MQ=255)
                                                                 aaGAGGCACTGGTTGAACAGCTGCGCGAAGCCAATGCTGCGCTGATTGCGCAATCGTATACCGACTCGTTAACCGGGCTACCTAATCGGCGGGCGATTTTTGAAAATCTGACGACACTGTTTTCCCTCGCCCGGCATCTTAACCATAAGa                                                                                 <  2:705394/150‑1 (MQ=255)
                                                                   gagGCACTGGTTGAACAGCTGCGCGAAGCCAATGCTGCGCTGATTGCGCAATCGTATACCGACTCGTTAACCGGGCTACCTAATCGGCGGGCGATTTTTGAAAATCTGACGACACTGTTTTCCCTCGCCCGGCATCTTAACCATAAGATa                                                                               >  2:4705339/1‑150 (MQ=255)
                                                                   gagGCACTGGTTGAACAGCTGCGCGAAGCCAATGCTGCGCTGATTGCGCAATCGTATACCGACTCGTTAACCGGGCTACCTAATCGGCGGGCGATTTTTGAAAATCTGACGACACTGTTTTCCCTCGCCCGGCATCTTAACCATAAGATa                                                                               >  1:1238714/1‑150 (MQ=255)
                                                                   gagGCACTGGTTGAACAGCTGCGCGAAGCCAATGCTGCGCTGATTGCGCAATCGTATACCGACTCGTTAACCGGGCTACCTAATCGGCGGGCGATTTTTGAAAATCTGACGACACTGTTTTCCCTCGCCCGGCATCTTAACCATAAGATa                                                                               >  2:4110181/1‑150 (MQ=255)
                                                                    agGCACTGGTTGAACAGCTGCGCGAAGCCAATGCTGCGCTGATTGCGCAATCGTATACCGACTCGTTAACCGGGCTACCTAATCGGCGGGCGATTTTTGAAAATCTGACGACACTGTTTTCCCTCGCCCGGCATCTTAACCATAAGATaa                                                                              >  1:1201590/1‑150 (MQ=255)
                                                                    agGCACTGGTTGAACAGCTGCGCGAAGCCAATGCTGCGCTGATTGCGCAATCGTATACCGACTCGTTAACCGGGCTACCTAATCGGCGGGCGATTTTTGAAAATCTGACGACACTGTTTTCCCTCGCCCGGCATCTTAACCATAAGATaa                                                                              >  1:3798041/1‑150 (MQ=255)
                                                                       cACTGGTTGAACAGCTGCGCGAAGCCAATGCTGCGCTGATTGCGCAATCGTATACCGACTCGTTAACCGGGCTACCTAATCGGCGGGCGATTTTTGAAAATCTGACGACACTGTTTTCCCTCGCCCGGCATCTTAACCATAAGATAATGa                                                                           <  2:2774262/150‑1 (MQ=255)
                                                                         cTGGTTGAACAGCTGCGCGAAGCCAATGCTGCGCTGATTGCGCAATCGTATACCGACTCGTTAACCGGGCTACCTAATCGGCGGGCGATTTTTGAAAATCTGACGACACTGTTTTCCCTCGCCCGGCATCTTAACCATAAGATAATGATc                                                                         >  1:982759/1‑150 (MQ=255)
                                                                          tGGTTGAACAGCTGCGCGAAGCCAATGCTGCGCTGATTGCGCAATCGTATACCGACTCGTTAACCGGGCTACCTAATCGGCGGGCGATTTTTGAAAATCTGACGACACTGTTTTCCCTCGCCCGGCATCTTAACCATAAGATAATGATcg                                                                        >  1:4799372/1‑150 (MQ=255)
                                                                              tGAACAGCTGCGCGAAGCCAATGCTGCGCTGATTGCGCAATCGTATACCGACTCGTTAACCGGGCTACCTAATCGGCGGGCGATTTTTGAAAATCTGACGACACTGTTTTCCCTCGCCCGGCATCTTAACCATAAGATAATGATCGCGtt                                                                    <  2:177968/150‑1 (MQ=255)
                                                                              tGAACAGCTGCGCGAAGCCAATGCTGCGCTGATTGCGCAATCGTATACCGACTCGTTAACCGGGCTACCTAATCGGCGGGCGATTTTTGAAAATCTGACGACACTGTTTTCCCTCGCCCGGCATCTTAACCATAAGATAATGATCGCGtt                                                                    >  1:4307898/1‑150 (MQ=255)
                                                                               gAACAGCTGCGCGAAGCCAATGCTGCGCTGATTGCGCAATCGTATACCGACTCGTTAACCGGGCTACCTAATCGGCGGGCGATTTTTGAAAATCTGACGACACTGTTTTCCCTCGCCCGGCATCTTAACCATAAGATAATGATCGCGttt                                                                   >  1:3704473/1‑150 (MQ=255)
                                                                                   aGCTGCGCGAAGCCAATGCTGCGCTGATTGCGCAATCGTATACCGACTCGTTAACCGGGCTACCTAATCGGCGGGCGATTTTTGAAAATCTGACGACACTGTTTTCCCTCGCCCGGCATCTTAACCATAAGATAATGATCGCGTTTATCg                                                               >  1:1165335/1‑150 (MQ=255)
                                                                                   aGCTGCGCGAAGCCAATGCTGCGCTGATTGCGCAATCGTATACCGACTCGTTAACCGGGCTACCGAATCGGCGGGCGATTTTTGAAAATCTGACGACACTGTTTTCCCTCGCCCGGCATCTTAACCATAAGATAATGATCGCGTTTATCg                                                               >  2:3669751/1‑150 (MQ=255)
                                                                                       gcgcGAAGCCAATGCTGCGCTGATTGCGCAATCGTATACCGACTCGTTAACCGGGCTACCTAATCGGCGGGCGATTTTTGAAAATCTGACGACACTGTTTTCCCTCGCCCGGCATCTTAACCATAAGATAATGATCGCGTTTATCGATCt                                                           <  1:2581030/150‑1 (MQ=255)
                                                                                           gAAGCCAATGCTGCGCTGATTGCGCAATCGTATACCGACTCGTTAACCGGGCTACCTAATCGGCGGGCGATTTTTGAAAATCTGACGACACTGTTTTCCCTCGCCCGGCATCTTAACCATAAGATAATGATCGCGTTTATCGATCTGGAt                                                       >  2:2555992/1‑150 (MQ=255)
                                                                                           gAAGCCAATGCTGCGCTGATTGCGCAATCGTATACCGACTCGTTAACCGGGCTACCTAATCGGCGGGCGATTTTTGAAAATCTGACGACACTGTTTTCCCTCGCCCGGCATCTTAACCATAAGATAATGATCGCGTTTATCGATCTGGAt                                                       >  1:3091326/1‑150 (MQ=255)
                                                                                           gAAGCCAATGCTGCGCTGATTGCGCAATCGTATACCGACTCGTTAACCGGGCTACCGAATCGGCGGGCGATTTTTGAAAATCTGACGACACTGTTTTCCCTCGCCCGGCATCTTAACCATAAGATAATGATCGCGTTTATCGATCTGGAt                                                       >  2:1052395/1‑150 (MQ=255)
                                                                                            aaGCCAATGCTGCGCTGATTGCGCAATCGTATACCGACTCGTTAACCGGGCTACCTAATCGGCGGGCGATTTTTGAAAATCTGACGACACTGTTTTCCCTCGCCCGGCATCTTAACCATAAGATAATGATCGCGTTTATCGATCTGGATa                                                      <  1:1042135/150‑1 (MQ=255)
                                                                                             aGCCAATGCTGCGCTGATTGCGCAATCGTATACCGACTCGTTAACCGGGCTACCTAATCGGCGGGCGATTTTTGAAAATCTGACGACACTGTTTTCCCTCGCCCGGCATCTTAACCATAAGATAATGATCGCGTTTATCGATCTGGATaa                                                     >  1:290279/1‑150 (MQ=255)
                                                                                              gCCAATGCTGCGCTGATTGCGCAATCGTATACCGACTCGTTAACCGGGCTACCTAATCGGCGGGCGATTTTTGAAAATCTGACGACACTGTTTTCCCTCGCCCGGCATCTTAACCATAAGATAATGATCGCGTTTATCGATCTGGATAAc                                                    <  2:4054352/150‑1 (MQ=255)
                                                                                              gCCAATGCTGCGCTGATTGCGCAATCGTATACCGACTCGTTAACCGGGCTACCTAATCGGCGGGCGATTTTTGAAAATCTGACGACACTGTTTTCCCTCGCCCGGCATCTTAACCATAAGATAATGATCGCGTTTATCGATCTGGATAAc                                                    <  2:2805340/150‑1 (MQ=255)
                                                                                                cAATGCTGCGCTGATTGCGCAATCGTATACCGACTCGTTAACCGGGCTACCTAATCGGCGGGCGATTTTTGAAAATCTGACGACACTGTTTTCCCTCGCCCGGCATCTTAACCATAAGATAATGATCGCGTTTATCGATCTGGATAACtt                                                  >  1:2267805/1‑150 (MQ=255)
                                                                                                 aaTGCTGCGCTGATTGCGCAATCGTATACCGACTCGTTAACCGGGCTACCTAATCGGCGGGCGATTTTTGAAAATCTGACGACACTGTTTTCCCTCGCCCGGCATCTTAACCATAAGATAATGATCGCGTTTATCGATCTGGATAACTTc                                                 >  1:1474031/1‑150 (MQ=255)
                                                                                                       gcgcTGATTGCGCAATCGTATACCGACTCGTTAACCGGGCTACCTAATCGGCGGGCGATTTTTGAAAATCTGACGACACTGTTTTCCCTCGCCCGGCATCTTAACCATAAGATAATGATCGCGTTTATCGATCTGGATAACTTCAAATTa                                           <  1:665187/150‑1 (MQ=255)
                                                                                                           tGATTGCGCAATCGTATACCGACTCGTTAACCGGGCTACCTAATCGGCGGGCGATTTTTGAAAATCTGACGACACTGTTTTCCCTCGCCCGGCATCTTAACCATAAGATAATGATCGCGTTTATCGATCTGGATAACTTCAAATTAATCa                                       >  2:875351/1‑150 (MQ=255)
                                                                                                           tGATTGCGCAATCGTATACCGACTCGTTAACCGGGCTACCTAATCGGCGGGCGATTTTTGAAAATCTGACGACACTGTTTTCCCTCGCCCGGCATCTTAACCATAAGATAATGATCGCGTTTATCGATCTGGATAACTTCAAATTAATCa                                       >  1:565938/1‑150 (MQ=255)
                                                                                                           tGATTGCGCAATCGTATACCGACTCGTTAACCGGGCTACCTAATCGGCGGGCGATTTTTGAAAATCTGACGACACTGTTTTCCCTCGCCCGGCATCTTAACCATAAGATAATGATCGCGTTTATCGATCTGGATAACTTCAAATTAATCa                                       >  2:1016641/1‑150 (MQ=255)
                                                                                                           tGATTGCGCAATCGTATACCGACTCGTTAACCGGGCTACCGAATCGGCGGGCGATTTTTGAAAATCTGACGACACTGTTTTCCCTCGCCCGGCATCTTAACCATAAGATAATGATCGCGTTTATCGATCTGGATAACTTCAAATTAATCa                                       <  1:1310190/150‑1 (MQ=255)
                                                                                                            gATTGCGCAATCGTATACCGACTCGTTAACCGGGCTACCTAATCGGCGGGCGATTTTTGAAAATCTGACGACACTGTTTTCCCTCGCCCGGCATCTTAACCATAAGATAATGATCGCGTTTATCGATCTGGATAACTTCAAATTAATCaa                                      >  2:952765/1‑150 (MQ=255)
                                                                                                             aTTGCGCAATCGTATACCGACTCGTTAACCGGGCTACCTAATCGGCGGGCGATTTTTGAAAATCTGACGACACTGTTTTCCCTCGCCCGGCATCTTAACCATAAGATAATGATCGCGTTTATCGATCTGGATAACTTCAAATTAATCAAt                                     <  1:1121562/150‑1 (MQ=255)
                                                                                                             aTTGCGCAATCGTATACCGACTCGTTAACCGGGCTACCTAATCGGCGGGCGATTTTTGAAAATCTGACGACACTGTTTTCCCTCGCCCGGCATCTTAACCATAAGATAATGATCGCGTTTATCGATCTGGATAACTTCAAATTAATCAAt                                     <  2:4270584/150‑1 (MQ=255)
                                                                                                              ttGCGCAATCGTATACCGACTCGTTAACCGGGCTACCTAATCGGCGGGCGATTTTTGAAAATCTGACGACACTGTTTTCCCTCGCCCGGCATCTTAACCATAAGATAATGATCGCGTTTATCGATCTGGATAACTTCAAATTAATCAATg                                    <  2:2407753/150‑1 (MQ=255)
                                                                                                                 cgcAATCGTATACCGACTCGTTAACCGGGCTACCTAATCGGCGGGCGATTTTTGAAAATCTGACGACACTGTTTTCCCTCGCCCGGCATCTTAACCATAAGATAATGATCGCGTTTATCGATCTGGATAACTTCAAATTAATCAATGATc                                 >  1:3475450/1‑150 (MQ=255)
                                                                                                                    aaTCGTATACCGACTCGTTAACCGGGCTACCTAATCGGCGGGCGATTTTTGAAAATCTGACGACACTGTTTTCCCTCGCCCGGCATCTTAACCATAAGATAATGATCGCGTTTATCGATCTGGATAACTTCAAATTAATCAATGATCGtt                              <  2:3371059/150‑1 (MQ=255)
                                                                                                                        gTATACCGTCTCGTTAACCGTGCTACCTAATCGGCGGGCGATTTTTGAAAATCTGACGACACTGTTTTACCTCGCCCGGCATCTTAACCATAAGATAATGATCGCGTTTATCGATCTGGATAACTTCAAATTAATCAATGATCGTTTTgg                          <  2:4764216/150‑1 (MQ=255)
                                                                                                                        gTATACCGACTCGTTAACCGGGCTACCTAATCGGCGGGCGATTTTTGAAAATCTGACGACACTGTTTTCCCTCGCCCGGCATCTTAACCATAAGATAATGATCGCGTTTATCGATCTGGATAACTTCAAATTAATCAATGATCGTTTTgg                          >  1:3145897/1‑150 (MQ=255)
                                                                                                                        gTATACCGACTCGTTAACCGGGCTACCGAATCGGCGGGCGATTTTTGAAAATCTGACGACACTGTTTTCCCTCGCCCGGCATCTTAACCCGAAGATAATGATCGCGTTTATCGATCTGGATAACTTCAAATTAATCAATGATCGTTTTgg                          >  2:3962500/1‑150 (MQ=255)
                                                                                                                        gTATACCGACTCGTTAACCGGGCTACCGAATCGGCGGGCGATTTTTGAAAATCTGACGACACTGTTTTCCCTCGCCCGGCATCTTAACCATAAGATAATGATCGCGTTTATCGATCTGGATAACTTCAAATTAATCAATGATCGTTTTgg                          >  2:1369726/1‑150 (MQ=255)
                                                                                                                          ataCCGACTCGTTAACCGGGCTACCTAATCGGCGGGCGATTTTTGAAAATCTGACGACACTGTTTTCCCTCGCCCGGCATCTTAACCATAAGATAATGATCGCGTTTATCGATCTGGATAACTTCAAATTAATCAATGATCGTTTTGGTc                        >  1:1603300/1‑150 (MQ=255)
                                                                                                                          ataCCGACTCGTTAACCGGGCTACCTAATCGGCGGGCGATTTTTGAAAATCTGACGACACTGTTTTCCCTCGCCCGGCATCTTAACCATAAGATAATGATCGCGTTTATCGATCTGGATAACTTCAAATTAATCAATGATCGTTTTGGTc                        >  1:4197741/1‑150 (MQ=255)
                                                                                                                           taCCGACTCGTTAACCGGGCTACCTAATCGGCGGGCGATTTTTGAAAATCTGACGACACTGTTTTCCCTCGCCCGGCATCTTAACCATAAGATAATGATCGCGTTTATCGATCTGGATAACTTCAAATTAATAAATGATCGTTTTGGTCa                       >  1:1879799/1‑150 (MQ=255)
                                                                                                                             ccGACTCGTTAACCGGGCTACCTAATCGGCGGGCGATTTTTGAAAATCTGACGACACTGTTTTCCCTCGCCCGGCATCTTAACCATAAGATAATGATCGCGTTTATCGATCTGGATAACTTCAAATTAATCAATGATCGTTTTGGTCata                     >  2:2219992/1‑150 (MQ=255)
                                                                                                                              cGACTCGTTAACCGGGCTACCTAATCGGCGGGCGATTTTTGAAAATCTGACGACACTGTTTTCCCTCGCCCGGCATCTTAACCATAAGATAATGATCGCGTTTATCGATCTGGATAACTTCAAATTAATCAATGATCGTTTTGGTCataa                    <  1:1429654/150‑1 (MQ=255)
                                                                                                                               gACTCGTTAACCGGGCTACCTAATCGGCGGGCGATTTTTGAAAATCTGACGACACTGTTTTCCCTCGCCCGGCATCTTAACCATAAGATAATGATCGCGTTTATCGATCTGGATAACTTCAAATTAATCAATGATCGTTTTGGTCataat                   >  1:4672037/1‑150 (MQ=255)
                                                                                                                               gACTCGTTAACCGGGCTACCGAATCGGCGGGCGATTTTTGAAAATCTGACGACACTGTTTTCCCTCGCCCGGCATCTTAACCATAAGATAATGATCGCGTTTATCGATCTGGATAACTTCAAATTAATCAATGATCGTTTTGGTCataat                   <  2:270174/150‑1 (MQ=255)
                                                                                                                                      tAACCGGGCTACCTAATCGGCGGGCGATTTTTGAAAATCTGACGACACTGTTTTCCCTCGCCCGGCATCTTAACCATAAGATAATGATCGCGTTTATCGATCTGGATAACTTCAAATTAATCAATGATCGTTTTGGTCATAATAGTGGCg            >  2:4758254/1‑150 (MQ=255)
                                                                                                                                      tAACCGGGCTACCTAATCGGCGGGCGATTTTTGAAAATCTGACGACACTGTTTTCCCTCGCCCGGCATCTTAACCATAAGATAATGATCGCGTTTATCGATCTGGATAACTTCAAATTAATCAATGATCGTTTTGGTCATAATAGTGGCg            <  2:3680474/150‑1 (MQ=255)
                                                                                                                                         ccGGGCTACCTAATCGGCGGGCGATTTTTGAAAATCTGACGACACTGTTTTCCCTCGCCCGGCATCTTAACCATAAGATAATGATCGCGTTTATCGATCTGGATAACTTCAAATTAATCAATGATCGTTTTGGTCATAATAGTGGCGATc         <  2:5007200/150‑1 (MQ=255)
                                                                                                                                          cGGGCTACCTAATCGGCGGGCGATTTTTGAAAATCTGACGACACTGTTTTCCCTCGCCCGGCATCTTAACCATAAGATAATGATCGCGTTTATCGATCTGGATAACTTCAAATTAATCAATGATCGTTTTGGTCATAATAGTGGCGATCt        >  2:1217516/1‑150 (MQ=255)
                                                                                                                                          cGGGCTACCGAATCGGCGGGCGATTTTTGAAAATCTGACGACACTGTTTTCCCTCGCCCGGCATCTTAACCATAAGATAATGATCGCGTTTATCGATCTGGATAACTTCAAATTAATCAATGATCGTTTTGGTCATAATAGTGGCGATCt        <  2:4823528/150‑1 (MQ=255)
                                                                                                                                           gggCTACCTAATCGGCGGGCGATTTTTGAAAATCTGACGACACTGTTTTCCCTCGCCCGGCATCTTAACCATAAGATAATGATCGCGTTTATCGATCTGGATAACTTCAAATTAATCAATGATCGTTTTGGTCATAATAGTGGCGATCTg       >  2:4650411/1‑150 (MQ=255)
                                                                                                                                              cTACCTAATCGGCGGGCGATTTTTGAAAATCTGACGACACTGTTTTCCCTCGCCCGGCATCTTAACCATAAGATAATGATCGCGTTTATCGATCTGGATAACTTCAAATTAATCAATGATCGTTTTGGTCATAATAGTGGCGATCTGttt    <  2:1467504/150‑1 (MQ=255)
                                                                                                                                               tACCTAATCGGCGGGCGATTTTTGAAAATCTGACGACACTGTTTTCCCTCGCCCGGCATCTTAACCATAAGATAATGATCGCGTTTATCGATCTGGATAACTTCAAATTAATCAAGGATCGTTTTGGTCATAATAGTGGCGATCTGTTtc   <  2:1882818/150‑1 (MQ=255)
                                                                                                                                               tACCGAATCGGCGGGCGATTTTTGAAAATCTGACGACACTGTTTTCCCTCGCCCGGCATCTTAACCATAAGATAATGATCGCGTTTATCGATCTGGATAACTTCAAATTAATCAATGATCGTTTTGGTCATAATAGTGGCGATCTGTTtc   <  1:5145116/150‑1 (MQ=255)
                                                                                                                                                aCCTAATCGGCGGGCGATTTTTGAAAATCTGACGACACTGTTTTCCCTCGCCCGGCATCTTAACCATAAGATAATGATCGCGTTTATCGATCTGGATAACTTCAAATTAATCAATGATCGTTTTGGTCATAATTGTGGCGATCTGTTtct  <  2:909612/150‑1 (MQ=255)
                                                                                                                                                aCCTAATCGGCGGGCGATTTTTGAAAATCTGACGACACTGTTTTCCCTCGCCCGGCATCTTAACCATAAGATAATGATCGCGTTTATCGATCTGGATAACTTCAAATTAATCAATGATCGTTTTGGTCATAATAGTGGCGATCTGTTtct  <  2:3475532/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
GTGGAGTGAACGCGCGGAACAGGTTTTACAGTTATTCGCCGGACTGATTGCACAATATATTCAAAAAGAGGCACTGGTTGAACAGCTGCGCGAAGCCAATGCTGCGCTGATTGCGCAATCGTATACCGACTCGTTAACCGGGCTACCGAATCGGCGGGCGATTTTTGAAAATCTGACGACACTGTTTTCCCTCGCCCGGCATCTTAACCATAAGATAATGATCGCGTTTATCGATCTGGATAACTTCAAATTAATCAATGATCGTTTTGGTCATAATAGTGGCGATCTGTTTCT  >  CP009273/1872373‑1872666

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑