Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,891,773 C→A 11.3% N195K (AAC→AAA)  sdaA → L‑serine dehydratase 1

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,891,7730CA11.3% 833.2 / 65.3 248N195K (AAC→AAA) sdaAL‑serine dehydratase 1
Reads supporting (aligned to +/- strand):  ref base C (111/109);  new base A (10/18);  total (121/127)
Fisher's exact test for biased strand distribution p-value = 1.63e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 265 total aligned reads displayed.

ATCGGCGGCGGTTTTATCGTCGATGAAGAACACTTTGGTCAGGATGCTGCCAACGAAGTAAGCGTGCCGTATCCGTTCAAATCTGCCACCGAACTGCTCGCGTACTGTAATGAAACCGGCTATTCGCTGTCTGGTCTCGCTATGCAGAACGAACTGGCGCTGCACAGCAAGAAAGAGATCGACGAGTATTTCGCGCATGTCTGGCAAACCATGCAGGCATGTATCGATCGCGGGATGAACACCGAAGGTGTACTGCCAGGCCCGCTGCGCGTGCCACGTCGTGCGTCTGCCCTGCGCCG  >  CP009273/1891624‑1891922
                                                                                                                                                     |                                                                                                                                                     
aTCGGCGGCGGTTTTATCGTCGATGAAGAACACTTTGGTCAGGATGCTGCCAACGAAGTAAGCGTGCCGTATCCGTTCAAATCTGCCACCGAACTGCTCGCGTACTGTAATGAAACCGGCTATTCGCTGTCTGGTCTCGCTATGCAgaac                                                                                                                                                       <  1:3038138/150‑1 (MQ=255)
aTCGGCGGCGGTTTTATCGTCGATGAAGAACACTTTGGTCAGGATGCTGCCAACGAAGTAAGCGTGCCGTATCCGTTCAAATCTGCCACCGAACTGCTCGCGTACTGTAATGAAACCGGCTATTCGCTGTCTGGTCTCGCTATGCAgaac                                                                                                                                                       <  1:1858557/150‑1 (MQ=255)
aTCGGCGGCGGTTTTATCGTCGATGAAGAACACTTTGGTCAGGATGCTGCCAACGAAGTAAGCGTGCCGTATCCGTTCAAATCTGCCACCGAACTGCTCGCGTACTGTAATGAAACCGGCTATTCGCTGTCTGGTCTCGCTATGCAgaac                                                                                                                                                       <  2:4378399/150‑1 (MQ=255)
aTCGGCGGCGGTTTTATCGTCGATGAAGAACACTTTGGTCAGGATGCTGCCAACGAAGTAAGCGTGCCGTATCCGTTCAAATCTGCCACCGAACTGCTCGCGGACTGTAATGAAACCGGCTATTCGCTGTCTGGTCTCGCTATGCAgaac                                                                                                                                                       >  1:1710337/1‑150 (MQ=255)
   ggcggcggTTTTATCGTCGATGAAGAACACTTTGGTCAGGATGCTGCCAACGAAGTAAGCGTGCCGTATCCGTTCAAATCTGCCACCGAACTGCTCGCGTACTGTAATGAAACCGGCTATTCGCTGTCTGGTCTCGCTATGCAgaacgaa                                                                                                                                                    <  1:4148699/150‑1 (MQ=255)
   ggcggcggTTTTATCGTCGATGAAGAACACATTGGTCAGGATGCTGCCAACGAAGTAAGCGTGCCGTATCCGTTCAAATCTGCCACCGAACAGCTCGCGTACTGTAATGAAACCGGCGATTCGCTGTCTGGTCTCGCTATGCAgaacgaa                                                                                                                                                    >  1:4423886/1‑150 (MQ=255)
    gcggcggTTTTATCGTCGATGAAGAACACTTTGGTCAGGATGCTGCCAACGAAGTAAGCGTGCCGTATCCGTTCAAATCTGCCACCGAACTGCTCGCGTACTGTAATGAAACCGGCTATTCGCTGTCTGGTCTCGCTATGCAgaacgaac                                                                                                                                                   <  2:4514645/150‑1 (MQ=255)
        cggTTTTATCGTCGATGAAGAACACTTTGGTCAGGATGCTGCCAACGAAGTAAGCGTGCCGTATCCGTTCAAATCTGCCACCGAACTGCTCGCGTACTGTAATGAAACCGGCTATTCGCTGTCTGGTCTCGCTATGCAGAACGAACTGgc                                                                                                                                               <  2:3317872/150‑1 (MQ=255)
        cggTTTTATCGTCGATGAAGAACACTTTGGTCAGGATGCTGCCAACGAAGTAAGCGTGCCGTATCCGTTCAAATCTGCCACCGAACTGCTCGCGTACTGTAATGAAACCGGCTATTCGCTGTCTGGTCTCGCTATGCAGAAAGAACTGgc                                                                                                                                               >  1:1356798/1‑150 (MQ=255)
         ggTTTTATCGTCGATGAAGAACACTTTGGTCAGGATGCTGCCAACGAAGTAAGCGTGCCGTATCCGTTCAAATCTGCCACCGAACTGCTCGCGTACTGTAATGAAACCGGCTATTCGCTGTCTGGTCTCGCTATGCAGAACGAACTGgcg                                                                                                                                              >  1:2658418/1‑150 (MQ=255)
          gTTTTATCGTCGATGAAGAACACTTTGGTCAGGATGCTGCCAACGAAGTAAGCGTGCCGTATCCGTTCAAATCTGCCACCGAACTGCTCGCGTACTGTAATGAAACCGGCTATTCGCTGTCTGGTCTCGCTATGCAGAACGAACTGgcgc                                                                                                                                             >  1:633958/1‑150 (MQ=255)
                tcgtcgATGAAGAACACTTTGGTCAGGATGCTGCCAACGAAGTAAGCGTGCCGTATCCGTTCAAATCTGCCACCGAACTGCTCGCGTACTGTAATGAAACCGGCTATTCGCTGTCTGGTCTCGCTATGCAGAACGAACTGGCGCTGcaca                                                                                                                                       >  1:2079551/1‑150 (MQ=255)
                 cgtcgATGAAGAACACTTTGGTCAGGATGCTGCCAACGAAGTAAGCGTGCCGTATCCGTTCAAATCTGCCACCGAACTGCTCGCGTACTGTAATGAAACCGGCTATTCGCTGTCTGGTCTCGCTATGCAGAAAGAACTGGCGCTGCACAg                                                                                                                                      <  1:4056212/150‑1 (MQ=255)
                 cgtcgATGAAGAACACTTTGGTCAGGATGCTGCCAACGAAGTAAGCGTGCCGTATCCGTTCAAATCTGCCACCGAACTGCTCGCGTACTGTAATGAAACCGGCTATTCGCTGTCTGGTCTCGCTATGCAGAAAGAACTGGCGCTGCACAg                                                                                                                                      <  1:4055943/150‑1 (MQ=255)
                   tcgATGAAGAACACTTTGGTCAGGATGCTGCCAACGAAGTAAGCGTGCCGTATCCGTTCAAATCTGCCACCGAACTGCTCGCGTACTGTAATGAAACCGGCTATTCGCTGTCTGGTCTCGCTATGCAGAACGAACTGGCGCTGCACAGCa                                                                                                                                    >  1:1564440/1‑150 (MQ=255)
                   tcgATGAAGAACACTTTGGTCAGGATGCTGCCAACGAAGTAAGCGTGCCGTATCCGTTCAAATCTGCCACCGAACTGCTCGCGTACTGTAATGAAACCGGCTATTCGCTGTCTGGTCTCGCTATGCAGAAAGAACTGGCGCTGCACAGCa                                                                                                                                    <  1:1423666/150‑1 (MQ=255)
                    cgATGAAGAACACTTTGGTCAGGATGCTGCCAACGAAGTAAGCGTGCCGTATCCGTTCAAATCTGCCACCGAACTGCTCGCGTACTGTAATGAAACCGGCTATTCGCTGTCTGGTCTCGCTATGCAGAACGAACTGGCGCTGCACAGCaa                                                                                                                                   <  2:1573805/150‑1 (MQ=255)
                       tGAAGAACACTTTGGTCAGGATGCTGCCAACGAAGTAAGCGTGCCGTATCCGTTCAAATCTGCCACCGAACTGCTCGCGTACTGTAATGAAACCGGCTATTCGCTGTCTGGTCTCGCTATGCAGAACGAACTGGCGCTGCACAGCaagaa                                                                                                                                >  1:3410033/1‑150 (MQ=255)
                        gaagaaCACTTTGGTCAGGATGCTGCCAACGAAGTAAGCGTGCCGTATCCGTTCAAATCTGCCACCGAACTGCTCGCGTACTGTAATGAAACCGGCTATTCGCTGTCTGGTCTCGCTATGCAGAACGAACTGGCGCTGCACAGCaagaaa                                                                                                                               <  2:5132497/150‑1 (MQ=255)
                          agaaCACTTTGGTCAGGATGCTGCCAACGAAGTAAGCGTGCCGTATCCGTTCAAATCTGCCACCGAACTGCTCGCGTACTGTAATGAAACCGGCTATTCGCTGTCTGGTCTCGCTATGCAGAACGAACTGGCGCTGCACAGCaagaaaga                                                                                                                             <  2:3543257/150‑1 (MQ=255)
                           gaaCACTTTGGTCAGGATGCTGCCAACGAAGTAAGCGTGCCGTATCCGTTCAAATCTGCCACCGAACTGCTCGCGTACTGTAATGAAACCGGCTATTCGCTGTCTGGTCTCGCTATGCAGAACGAACTGGCGCTGCACAGCACGAAagag                                                                                                                            >  1:4544984/1‑150 (MQ=255)
                            aaCACTTTGGTCAGGATGCTGCCAACGAAGTAAGCGTGCCGTATCCGTTCAAATCTGCCACCGAACTGCTCGCGTACTGTCATGAAACCGGCTATTCGCTGTCTGGTCTCGCTCTGCAGAACGAACTGGCGCTGCACAGCAAGAAAgaga                                                                                                                           >  2:4335204/1‑150 (MQ=255)
                              cacTTTGGTCAGGATGCTGCCAACGAAGTAAGCGTGCCGTATCCGTTCAAATCTGCCACCGAACTGCTCGCGTACTGTAATGAAACCGGCTATTCGCTGTCTGGTCTCGCTATGCAGAACGAACTGGCGCTGCACAGCAAGAAAGAGATc                                                                                                                         >  2:292073/1‑150 (MQ=255)
                              cacTTTGGTCAGGATGCTGCCAACGAAGTAAGCGTGCCGTATCCGTTCAAATCTGCCACCGAACTGCTCGCGTACTGTAATGAAACCGGCTATTCGCTGTCTGGTCTCGCTATGCAGAACGAACTGGCGCTGCACAGCAAGAAAGAGATc                                                                                                                         <  2:784941/150‑1 (MQ=255)
                                cTTTGGTCAGGATGCTGCCAACGAAGTAAGCGTGCCGTATCCGTTCAAATCTGCCACCGAACTGCTCGCATACTGTAATGAAACCGGCTATTCGCTGTCTGGTCTCGCTATGCAGAACGAACTGGCGCTGCACAGCAAGAAAGAGATcga                                                                                                                       >  1:1293183/1‑150 (MQ=255)
                                 tttGGTCAGGATGCTGCCAACGAAGTAAGCGTGCCGTATCCGTTCAAATCTGCCACCGAACTGCTCGCGTACTGTAATGAAACCGGCTATTCGCTGTCTGGTCTCGCTATGCAGAACGAACTGGCGCTGCACAGCAAGAAAGAGATcgac                                                                                                                      >  2:3641887/1‑150 (MQ=255)
                                 tttGGTCAGGATGCTGCCAACGAAGTAAGCGTGCCGTATCCGTTCAAATCTGCCACCGAACTGCTCGCGTACTGTAATGAAACCGGCTATTCGCTGTCTGGTCTCGCTATGCAGAACGAACTGGCGCTGCACAGCAAGAAAGAGATcgac                                                                                                                      <  2:3004026/150‑1 (MQ=255)
                                       cAGGATGCTGCCAACTAAGTAAGCGTGCCGTATCCGTTCAAATCTGCCACCGAACTGCTCGCGTACTGTAATGAAACCGGCTATTCGCTGTCTGGTCTCGCTATGCAGAACGAACTGGCGCTGCACAGCAAGAAAGAGATCGACGAGTAt                                                                                                                <  1:3079950/150‑1 (MQ=255)
                                         ggATGCTGCCAACGAAGTAAGCGTGCCGTATCCGTTCAAATCTGCCACCGAACTGCTCGCGTACTGTAATGAAACCGGCTATTCGCTGTCTGGTCTCGCTATGCAGAACGAACTGGCGCTGCACAGCAAGAAAGAGATCGACGAGTAttt                                                                                                              >  2:4088938/1‑150 (MQ=255)
                                          gATGCTGCCAACGAAGTAAGCGTGCCGGATCCGTTCAAATCAGCCACCGAACTGCTCGCGTAATGTAATGAAACCGGCTATTCGCGGTCTGGTCTCGCTATGCAGAACGAACTGGCGCTGCACAGCAAGAAAGAGATCGACGAGTATTTc                                                                                                             <  2:4168851/150‑1 (MQ=255)
                                           aTGCTGCCAACGAAGTAAGCGTGCCGTATCCGTTCAAATCTGCCACCGAACTGCTCGCGTACTGTAATGAAACCGGCTATTCGCTGTCTGGTCTCGCTATGCAGAACGAACTGGCGCTGCACAGCAAGAAAGAGATCGACGAGTATTTcg                                                                                                            <  2:4020696/150‑1 (MQ=255)
                                           aTGCTGCCAACGAAGTAAGCGTGCCGTATCCGTTCAAATCTGCCACCGAACTGCTCGCGTACTGTAATGAAACCGGCTATTCGCTGTCTGGTCTCGCTATGCAGAACGAACTGGCGCTGCACAGCAAGAAAGAGATCGACGAGTATTTcg                                                                                                            <  1:3733567/150‑1 (MQ=255)
                                            tgctgcCAACGAAGTAAGCGTGCCGTATCCGTTCAAATCTGCCACCGAACTGCTCGCGTACTGTAATGAAACCGGCTATTCGCTGTCTGGTCTCGCTATGCAGAACGAACTGGCGCTGCACAGCAAGAAAGAGATCGACGAGTATTTcgc                                                                                                           >  1:4708656/1‑150 (MQ=255)
                                                gcCAACGAAGTAAGCGTGCCGTATCCGTTCAAATCTGCCACCGAACTGCTCGCGTACTGTAATGAAACCGGCTATTCGCTGTCTGGTCTCGCTATGCAGAACGAACTGGCGCTGCACAGCAAGAAAGAGATCGACGAGTATTTCGCGCAt                                                                                                       <  1:5078804/150‑1 (MQ=255)
                                                gcCAACGAAGTAAGCGTGCCGTATCCGTTCAAATCTGCCACCGAACTGCTCGCGTACTGTAATGAAACCGGCTATTCGCTGTCTGGTCTCGCTATGCAGAACGAACTGGCGCTGCACAGCAAGAAAGAGATCGACGAGTATTTCGCGCAt                                                                                                       >  1:2350648/1‑150 (MQ=255)
                                                gcCAACGAAGTAAGCGTGCCGTATCCGTTCAAATCTGCCACCGAACTGCTCGCGTACTGTAATGAAACCGGCTATTCGCTGTCTGGTCTCGCTATGCAGAACGAACTGGCGCTGCACAGCAAGAAAGAGATCGACGAGTATTTCGCGCAt                                                                                                       >  2:1907539/1‑150 (MQ=255)
                                                 ccAACGAAGTAAGCGTGCCGTATCCGTTCAAATCTGCCACCGAACTGCTCGCGTACTGTAATGAAACCGGCTATTCGCTGTCTGGTCTCGCTATGCAGAACGAACTGGCGCTGCACAGCAAGAAAGAGATCGACGAGTATTTCGCGCATg                                                                                                      >  1:93498/1‑150 (MQ=255)
                                                   aaCGAAGTAAGCGTGCCGTATCCGTTCAAATCTGCCACCGAACTGCTCGCGTACTGTAATGAAACCGGCTATTCGCTGTCTGGTCTCGCTATGCAGAACGAACTGGCGCTGCACAGCAAGAAAGAGATCGACGAGTATTTCGCGCATGTc                                                                                                    <  1:2725836/150‑1 (MQ=255)
                                                    aCGAAGTAAGCGTGCCGTATCCGTTCAAATCTGCCACCGAACTGCTCGCGTACTGTAATGAAACCGGCTATTCGCTGTCTGGTCTCGCTATGCAGAACGAACTGGCGCTGCACAGCAAGAAAGAGATCGACGAGTATTTCGCGCATGTCt                                                                                                   >  2:3999002/1‑150 (MQ=255)
                                                     cGAAGTAAGCGTGCCGTATCCGTTCAAATCTGCCACCGAACTGCTCGCGTACTGTAATGAAACCGGCTATTCGCTGTCTGGTCTCGCTATGCAGAACGAACTGGCGCTGCACAGCAAGAAAGAGATCGACGAGTATTTCGCGCATGTCTg                                                                                                  <  1:3249277/150‑1 (MQ=255)
                                                      gAAGTAAGCGTGCCGTATCCGTTCAAATCTGCCACCGAACTGCTCGCGTACTGTAATGAAACCGGCTATTCGCTGTCTGGTCTCGCTATGCAGAACGAACTGGCGCTGCACAGCAAGAAAGAGATCGACGAGTATTTCGCGCATGTCTgg                                                                                                 >  1:4253060/1‑150 (MQ=255)
                                                         gTAAGCGTGCCGTATCCGTTCAAATCTGCCACCGAACTGCTCGCGTACTGTAATGAAACCGGCTATTCGCTGTCTGGTCTCGCTATGCAGAACGAACTGGCGCTGCACAGCAAGAAAGAGATCGACGAGTATTTCGCGCATGTCTGGCaa                                                                                              <  1:1691129/150‑1 (MQ=255)
                                                         gTAAGCGTGCCGTATCCGTTCAAATCTGCCACCGAACTGCTCGCGTACTGTAATGAAACCGGCTATTCGCTGTCTGGTCTCGCTATGCAGAACGAACTGGCGCTGCACAGCAAGAAAGAGATCGACGAGTATTTCGCGCATGTCTGGCaa                                                                                              >  1:814571/1‑150 (MQ=255)
                                                            aGCGTGCCGTATCCGTTCAAATCTGCCACCGAACTGCTCGCGTACTGTAATGAAACCGGCTATTCGCTGTCTGGTCTCGCTATGCAGAACGAACTGGCGCTGCACAGCAAGAAAGAGATCGACGAGTATTTCGCGCATGTCTGGCAAAcc                                                                                           <  2:505132/150‑1 (MQ=255)
                                                             gCGTGCCGTATCCGTTCAAATCTGCCACCGAACTGCTCGCGTACTGTAATGAAACCGGCTATTCGCTGTCTGGTCTCGCTATGCAGAACGAACTGGCGCTGCACAGCAAGAAAGAGATCGACGAGTATTTCGCGCATGTCTGGCAAACCa                                                                                          <  1:1032968/150‑1 (MQ=255)
                                                              cGTGCCGTATCCGTTCAAATCTGCCACCGAACTGCTCGCGTACTGTAATGAAACCGGCTATTCGCTGTCTGGTCTCGCTATGCAGAACGAACTGGCGCTGCACAGCAAGAAAGAGATCGACGAGTATTTCGCGCATGTCTGGCAAACCAt                                                                                         >  2:85367/1‑150 (MQ=255)
                                                               gTGCCGTATCCGTTCAAATCTGCCACCGAACTGCTCGCGTACTGTAATGAAACCGGCTATTCGCTGTCTGGTCTCGCTATGCAGAACGAACTGGCGCTGCACAGCAAGAAAGAGATCGACGAGTATTTCGCGCATGTCTGGCAAACCATg                                                                                        <  1:4478195/150‑1 (MQ=255)
                                                                 gCCGTATCCGTTCAAATCTGCCACCGAACTGCTCGCGTACTGTAATGAAACCGGCTATTCGCTGTCTGGTCTCGCTATGCAGAACGAACTGGCGCTGCACAGCAAGAAAGAGATCGACGAGTATTTCGCGCATGTCTGGCAAACCATGCa                                                                                      <  2:3697897/150‑1 (MQ=255)
                                                                  ccGTATCCGTTCAAATCTGCCACCGAACTGCTCGCGTACTGTAATGAAACCGGCTATTCGCTGTCTGGTCTCGCTATGCAGAACGAACTGGCGCTGCACAGCAAGAAAGAGATCGACGAGTATTTCGCGCATGTCTGGCAAACCATGCAg                                                                                     <  2:429251/150‑1 (MQ=255)
                                                                   cGTATCCGTTCAAATCTGCCACCGAACTGCTCGCGTACTGTAATGAAACCGGCTATTCGCTGTCTGGTCTCGCTATGCAGAACGAACTGGCGCTGCACAGCAAGAAAGAGATCGACGAGTATTTCGCGCATGTCTGGCAAACCATGCAgg                                                                                    <  1:1145771/150‑1 (MQ=255)
                                                                     tATCCGTTCAAATCTGCCACCGAACTGCTCGCGTACTGTAATGAAACCGGCTATTCGCTGTCTGGTCTCGCTATGCAGAACGAACTGGCGCTGCACAGCAAGAAAGAGATCGACGAGTATTTCGCGCATGTCTGGCAAACCATGCAGGCa                                                                                  >  1:2182723/1‑150 (MQ=255)
                                                                      aTCCGTTCAAATCTGCCACCGAACTGCTCGCGTACTGTAATGAAACCGGCTATTCGCTGTCTGGTCTCGCTATGCAGAACGAACTGGCGCTGCACAGCAAGAAAGAGATCGACGAGTATTTCGCGCATGTCTGGCAAACCATGCAGGCAt                                                                                 >  2:2486137/1‑150 (MQ=255)
                                                                      aTCCGTTCAAATCTGCCACCGAACTGCTCGCGTACTGTAATGAAACCGGCTATTCGCTGTCTGGTCTCGCTATGCAGAACGAACTGGCGCTGCACAGCAAGAAAGAGATCGACGAGTATTTCGCGCATGTCTGGCAAACCATGCAGGCAt                                                                                 <  2:1778872/150‑1 (MQ=255)
                                                                        ccGTTCAAATCTGCCACCGAACTGCTCGCGTACTGTAATGAAACCGGCTATTCGCTGTCTGGTCTCGCTATGCAGAACGAACTGGCGCTGCACAGCAAGAAAGAGATCGACGAGTATTTCGCGCATGTCTGGCAAACCATGCAGGCATGt                                                                               >  2:585201/1‑150 (MQ=255)
                                                                        ccGTTCAAATCTGCCACCGAACTGCTCGCGTACTGTAATGAAACCGGCTATTCGCTGTCTGGTCTCGCTATGCAGAAAGAACTGGCGCTGCACAGCAAGAAAGAGATCGACGAGTATTTCGCGCATGTCTGGCAAACCATGCAGGCATGt                                                                               >  1:574634/1‑150 (MQ=255)
                                                                          gTTCAAATCTGCCACCGAACTGCTCGCGTACTGTAATGAACCCGGCTATTCGCTGTCTGGTCTCGCTATGCAGAACGAACTGGCGCTGCACAGCAAGAAAGAGATCGACGAGTATTTCGCGCATGTCTGGCAAACCATGCAGGCATGTat                                                                             <  2:2619159/150‑1 (MQ=255)
                                                                          gTTCAAATCTGCCACCGAACTGCTCGCGTACTGTAATGAAACCGGCTATTCGCTGTCTGGTCTCGCTATGCAGAACGAACTGGCGCTGCACAGCAAGAAAGAGATCGACGAGTATTTCGCGCATGTCTGGCAAACCATGCAGGCATGTat                                                                             <  2:3163207/150‑1 (MQ=255)
                                                                            tCAAATCTGCCACCGAACTGCTCGCGTACTGTAATGAAACCGGCTATTCGCTGTCTGGTCTCGCTATGCAGAACGAACTGGCGCTGCACAGCAAGAAAGAGATCGACGAGTATTTCGCGCATGTCTGGCAAACCATGCAGGCATGTatcg                                                                           >  1:1013434/1‑150 (MQ=255)
                                                                             cAAATCTGCCACCGAACTGCTCGCGTACTGTAATGAAACCGGCTATTCGCTGTCTGGTCTCGCTATGCAGAAAGAACTGGCGCTGCACAGCAAGAAAGAGATCGACGAGTATTTCGCGCATGTCTGGCAAACCATGCAGGCATGTatcga                                                                          <  2:441684/150‑1 (MQ=255)
                                                                              aaaTCTGCCACCGAACTGCTCGCGTACTGTAATGAAACCGGCTATTCGCTGTCTGGTCTCGCTATGCAGAACGAACTGGCGCTGCACAGCAAGAAAGAGATCGACGAGTATTTCGCGCATGTCTGGCAAACCATGCAGGCATGTatcgat                                                                         >  1:1116604/1‑150 (MQ=255)
                                                                               aaTCTGCCACCGAACTGCTCGCGTACTGTAATGAAACCGGCTATTCGCTGTCTGGTCTCGCTATGCAGAACGAACTGGCGCTGCACAGCAAGAAAGAGATCGACGAGTATTTCGCGCATGTCTGGCAAACCATGCAGGCATGTatcgatc                                                                        >  2:1822537/1‑150 (MQ=255)
                                                                                      cACCGAACTGCTCGCGTACTGTAATGAAACCGGCTATTCGCTGTCTGGTCTCGCTATGCAGAACGAACTGGCGCTGCACAGCAAGAAAGAGATCGACGAGTATTTCGCGCATGTCTGGCAAACCATGCAGGCATGTATCGATCGCGGGAt                                                                 <  1:1263042/150‑1 (MQ=255)
                                                                                        ccGAACTGCTCGCGTACTGTAATGAAACCGGCTATTCGCTGTCTGGTCTCGCTATGCAGAACGAACTGGCGCTGCACAGCAAGAAAGAGATCGACGAGTATTTCGCGCATGTCTGGCAAACCATGCAGGCATGTATCGATCGCGGGATGa                                                               <  1:4807994/150‑1 (MQ=255)
                                                                                         cGAACTGCTCGCGTACTGTAATGACACCGGCTATTCGCTGTCTGGTCTCGCTAGGCAGAACGAACTGGCGCTGCACAGCAAGAAAGAGATCGACGAGTATTTCGCGCATGTCTGGCAAACCATGCAGGCATGTATCGATCGCGGGATGaa                                                              <  2:2856546/150‑1 (MQ=255)
                                                                                          gAACTGCTCGCGTACTGTAATGAAACCGGCTATTCGCTGTCTGGTCTCGCTATGCAGAACGAACTGGCGCTGCACAGCAAGAAAGAGATCGACGAGTATTTCGCGCATGTCTGGCAAACCATGCAGGCATGTATCGATCGCGGGATGAac                                                             <  2:2182723/150‑1 (MQ=255)
                                                                                          gAACTGCTCGCGTACTGTAATGAAACCGGCTATTCGCTGTCTGGTCTCGCTATGCAGAACGAACTGGCGCTGCACAGCAAGAAAGAGATCGACGAGTATTTCGCGCATGTCTGGCAAACCATGCAGGCATGTATCGATCGCGGGATGAac                                                             >  1:1610498/1‑150 (MQ=255)
                                                                                          gAACTGCTCGCGTACTGTAATGAAACCGGCTATTCGCTGTCTGGTCTCGCTATGCAGAACGAACTGGCGCTGCACAGCAAGAAAGAGATCGACGAGTATTTCGCGCATGTCTGGCAAACCATGCAGGCATGTATCGATCGCGGGATGAac                                                             >  1:3261170/1‑150 (MQ=255)
                                                                                             cTGCTCGCGTACTGTAATGAAACCGGCTATTCGCTGTCTGGTCTCGCTATGCAGAACGAACTGGCGCTGCACAGCAAGAAAGAGATCGACGAGTATTTCGCGCATGTCTGGCAAACCATGCAGGCATGTATCGATCGCGGGATGAACAcc                                                          >  2:2405093/1‑150 (MQ=255)
                                                                                                     gTACTGTAATGAAACCGGCTATTCGCTGTCTGGTCTCGCTATGCAGAACGAACTGGCGCTGCACAGCAAGAAAGAGATCGACGAGTATTTCGCGCATGTCTGGCAAACCATGCAGGCATGTATCGATCGCGGGATGAACACCGAAGgtgt                                                  <  2:4871945/150‑1 (MQ=255)
                                                                                                     gTACTGTAATGAAACCGGCTATTCGCTGTCTGGTCTCGCTATGCAGAAAGAACTGGCGCTGCACAGCAAGAAAGAGATCGACGAGTATTTCGCGCATGTCTGGCAAACCATGCAGGCATGTATCGATCGCGGGATGAACACCGAAGgtgt                                                  <  2:1356798/150‑1 (MQ=255)
                                                                                                       aCTGTAATGAAACCGGCTATTCGCTGTCTGGTCTCGCTATGCAGAACGAACTGGCGCTGCACAGCAAGAAAGAGATCGACGAGTATTTCGCGCATGTCTGGCAAACCATGCAGGCATGTATCGATCGCGGGATGAACACCGAAGGTGTAc                                                >  1:2500411/1‑150 (MQ=255)
                                                                                                           tAATGAAACCGGCTATTCGCTGTCTGGTCTCGCTATGCAGAACGAACTGGCGCTGCACAGCAAGAAAGAGATCGACGAGTATTTCGCGCATGTCTGGCAAACCATGCAGGCATGTATCGATCGCGGGATGAACACCGAAGGTGTACTGcc                                            <  2:2984466/150‑1 (MQ=255)
                                                                                                             aTGAAACCGGCTATTCGCTGTCTGGTCTCGCTATGCAGAACGAACTGGCGCTGCACAGCAAGAAAGAGATCGACGAGTATTTCGCGCATGTCTGGCAAACCATGCAGGCATGTATCGATCGCGGGATGAACACCGAAGGTGTACTGCCAg                                          <  1:3802057/150‑1 (MQ=255)
                                                                                                               gAAACCGGCTATTCGCTGTCTGGTCTCGCTATGCAGAACGAACTGGCGCTGCACAGCAAGAAAGAGATCGACGAGTATTTCGCGCATGTCTGGCAAACCATGCAGGCATGTATCGATCGCGGGATGAACACCGAAGGTGTACTGCCAGGc                                        >  2:3944445/1‑150 (MQ=255)
                                                                                                                aaaCCGGCTATTCGCTGTCTGGTCTCGCTATGCAGAACGAACTGGCGCTGCACAGCAAGAAAGAGATCGACGAGTATTTCGCGCATGTCTGGCAAACCATGCAGGCATGTATCGATCGCGGGATGAACACCGAAGGTGTACTGCCAGGcc                                       >  1:2570546/1‑150 (MQ=255)
                                                                                                                  aCCGGCTATTCGCTGTCTGGTCTCGCTATGCAGAACGAACTGGCGCTGCACAGCAAGAAAGAGATCGACGAGTATTTCGCGCATGTCTGGCAAACCATGCAGGCATGTATCGATCGCGGGATGAACACCGAAGGTGTACTGCCAGGCCCg                                     >  1:1956047/1‑150 (MQ=255)
                                                                                                                       cTATTCGCTGTCTGGTCTCGCTATGCAGAAAGAACTGGCGCTGCACAGCAAGAAAGAGATCGACGAGTATTTCGCGCATGTCTGGCAAACCATGCAGGCATGTATCGATCGCGGGATGAACACCGAAGGTGTACTGCCAGGCCCGCTgcg                                >  1:3172788/1‑150 (MQ=255)
                                                                                                                        tATTCGCTGTCTGGTCTCGCTATGCAGAACGAACTGGCGCTGCACAGCAAGAAAGAGATCGACGAGTATTTCGCGCATGTCTGGCAAACCATGCAGGCATGTATCGATCGCGGGATGAACACCGAAGGTGTACTGCCAGGCCCGCTgcgc                               >  2:145899/1‑150 (MQ=255)
                                                                                                                        tATTCGCTGTCTGGTCTCGCTATGCAGAACGAACTGGCGCTGCACAGCAAGAAAGAGATCGACGAGTATTTCGCGCATGTCTGGCAAACCATGCAGGCATGTATCGATCGCGGGATGAACACCGAAGGTGTACTGCCAGGCCCGCTgcgc                               >  2:2763801/1‑150 (MQ=255)
                                                                                                                        tATTCGCTGTCTGGTCTCGCTATGCAGAACGAACTGGCGCTGCACAGCAAGAAAGAGATCGACGAGTATTTCGCGCATGTCTGGCAAACCATGCAGGCATGTATCGATCGCGGGATGAACACCGAAGGTGTACTGCCAGGCCCGCTgcgc                               >  1:3707588/1‑150 (MQ=255)
                                                                                                                        tATTCGCTGTCTGGTCTCGCTATGCAGAACGAACTGGCGCTGCACAGCAAGAAAGAGATCGACGAGTATTTCGCGCATGTCTGGCAAACCATGCAGGCATGTATCGATCGCGGGATGAACACCGAAGGTGTACTGCCAGGCCCGCTgcgc                               <  2:3569871/150‑1 (MQ=255)
                                                                                                                               tGTCTGGTCTCGCTATGCAGAACGAACTGGCGCTGCACAGCAAGAAAGAGATCGCCGAGTATTTCGCGCATGTCTGGCAAACCATGCAGGCATGTATCGATCGCGGGATGAACACCGAAGGTGTACTGCCAGGCCCGCTGCGCGTGCCAc                        <  2:5159888/150‑1 (MQ=255)
                                                                                                                                   tGGTCTCGCTATGCAGAAAGAACTGGCGCTGCACAGCAAGAAAGAGATCGACGAGTATTTCGCGCATGTCTGGCATACCATGCAGGCATGTATCGATCGCGGGATGAACACCGAAGGTGTACTGCCAGGCCCGCTGCGCGTGCCAcgtcg                    <  2:2684471/150‑1 (MQ=255)
                                                                                                                                   tGGTCTCGCTATGCAGAAAGAACTGGCGCTGCACAGCAAGAAAGAGATCGACGAGTATTTCGCGCATGTCTGGCAAACCATGCAGGCATGTATCGATCGCGGGATGAACACCGAAGGTGTACTGCCAGGCCCGCTGCGCGTGCCAcgtcg                    <  2:483409/150‑1 (MQ=255)
                                                                                                                                    ggTCTCGCTATGCAGAACGAACTGGCGCTGCACAGCAAGAAAGAGATCGACGAGTATTTCGCGCATGTCTGGCAAACCATGCAGGCATGTATCGATCGCGGGATGAACACCGAAGGTGTACTGCCAGGCCCGCTGCGCGTGCCAcgtcgt                   >  2:1518729/1‑150 (MQ=255)
                                                                                                                                     gTCTCGCTATGCAGAACGAACTGGCGCTGCACAGCAAGAAAGAGATCGACGAGTATTTCGCGCATGTCTGGCAAACCATGCAGGCATGTATCGATCGCGGGATGAACACCGAAGGTGTACTGCCAGGCCCGCTGCGCGTGCCACGTCGTg                  <  2:3410033/150‑1 (MQ=255)
                                                                                                                                     gTCTCGCTATGCAGAACGAACTGGCGCTGCACAGCAAGAAAGAGATCGACGAGTATTTCGCGCATGTCTGGCAAACCATGCAGGCATGTATCGATCGCGGGATGAACACCGAAGGTGTACTGCCAGGCCCGCTGCGCGTGCCACGTCGTg                  <  2:1052597/150‑1 (MQ=255)
                                                                                                                                       ctcGCTATGCAGAACGAACTGGCGCTGCACAGCAAGAAAGAGATCGACGAGTATTTCGCGCATGTCTGGCAAACCATGCAGGCATGTATCGATCGCGGGATGAACACCGAAGGTGTACTGCCAGGCCCGCTGCGCGTGCCACGTCGTGCg                >  1:2858375/1‑150 (MQ=255)
                                                                                                                                         cGCTATGCAGAACGAACTGGCGCTGCACAGCAAGAAAGAGATCGACGAGTATTTCGCGCATGTCTGGCAAACCATGCAGGCATGTATCGATCGCGGGATGAACACCGAAGGTGTACTGCCAGGCCCGCTGCGCGTGCCACGTCGTGCGTc              >  1:4113831/1‑150 (MQ=255)
                                                                                                                                         cGCTATGCAGAACGAACTGGCGCTGCACAGCAAGAAAGAGATCGACGAGTATTTCGCGCATGTCTGGCAAACCATGCAGGCATGTATCGATCGCGGGATGAACACCGAAGGTGTACTGCCAGGCCCGCTGCGCGTGCCACGTCGTGCGTc              >  2:1145112/1‑150 (MQ=255)
                                                                                                                                         cGCTATGCAGAACGAACTGGCGCTGCACAGCAAGAAAGAGATCGACGAGTATTTCGCGCATGTCTGGCAAACCATGCAGGCATGTAGCGATCGCGGGATGAACACCGAAGGTGTACTGCCAGGCCCGCTGCGCGTGCCACGTCGTGCGTc              >  2:3049219/1‑150 (MQ=255)
                                                                                                                                          gCTATGCAGAACGAACTGGCGCTGCACAGCAAGAAAGAGATCGACGAGTATTTCGCGCATGTCTGGCAAACCATGCAGGCATGTATCGATCGCGGGATGAACACCGAAGGTGTACTGCCAGGCCCGCTGCGCGTGCCACGTCGTGCGTCt             <  2:1558252/150‑1 (MQ=255)
                                                                                                                                          gCTATGCAGAACGAACTGGCGCTGCACAGCAAGAAAGAGATCGACGAGTATTTCGCGCATGTCTGGCAAACCATGCAGGCATGTATCGATCGCGGGATGAACACCGAAGGTGTACTGCCAGGCCCGCTGCGCGTGCCACGTCGTGCGTCt             <  2:1293183/150‑1 (MQ=255)
                                                                                                                                          gCTATGCAGAAAGAACTGGCGCTGCACAGCAAGAAAGAGATCGACGAGTATTTCGCGCATGTCTGGCAAACCATGCAGGCATGTATCGATCGCGGGATGAACACCGAAGGTGTACTGCCAGGCCCGCTGCGCGTGCCACGTCGTGCGTCt             <  2:1276092/150‑1 (MQ=255)
                                                                                                                                               gCAGAACGAACTGGCGCTGCACAGCAAGAAAGAGATCGACGAGTATTTCGCGCATGTCTGGCAAACCATGCAGGCATGTATCGATCGCGGGATGAACACCGAAGGTGTACTGCCAGGCCCGCTGCGCGTGCCACGTCGTGCGTCTGCCCt        <  2:2568160/150‑1 (MQ=255)
                                                                                                                                                cAGAACGAACTGGCGCTGCACAGCAAGAAAGAGATCGACGAGTATTTCGCGCATGTCTGGCAAACCATGCAGGCATGTATCGATCGCGGGATGAACACCGAAGGTGTACTGCCAGGCCCGCTGCGCGTGCCACGTCGTGCGTCTGCCCTg       >  1:1616545/1‑150 (MQ=255)
                                                                                                                                                 aGAACGAACTGGCGCTGCACAGCAAGAAAGAGATCGACGAGTATTTCGCGCATGTCTGGCAAACCATGCAGGCATGTATCGATCGCGGGATGAACACCGAAGGTGTACTGCCAGGCCCGCTGCGCGTGCCACGTCGTGCGTCTGCCCTgc      >  1:2614123/1‑150 (MQ=255)
                                                                                                                                                  gaacgaacTGGCGCTGCACAGCAAGAAAGAGATCGACGAGTATTTCGCGCATGTCTGGCAAACCATGCAGGCATGTATCGATCGCGGGATGAACACCGAAGGTGTACTGCCAGGCCCGCTGCGCGTGCCACGTCGTGCGTCTGCCCTgcg     <  1:842449/150‑1 (MQ=255)
                                                                                                                                                  gaaagaacTGGCGCTGCACAGCAAGAAAGAGATCGACGAGTATTTCGCGCATGTCTGGCAAACCATGCAGGCATGTATCGATCGCGGGATGAACACCGAAGGTGTACTGCCAGGCCCGCTGCGCGTGCCACGTCGTGCGTCTGCCCTgcg     >  1:1893953/1‑150 (MQ=255)
                                                                                                                                                    acgaacTGGCGCTGCACAGCAAGAAAGAGATCGACGAGTATTTCGCGCATGTCTGGCAAACCATGCAGGCATGTATCGATCGCGGGATGAACACCGAAGGTGTACTGCCAGGCCCGCTGCGCGTGCCACGTCGTGCGTCTGCCCTGCGcc   <  2:660414/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
ATCGGCGGCGGTTTTATCGTCGATGAAGAACACTTTGGTCAGGATGCTGCCAACGAAGTAAGCGTGCCGTATCCGTTCAAATCTGCCACCGAACTGCTCGCGTACTGTAATGAAACCGGCTATTCGCTGTCTGGTCTCGCTATGCAGAACGAACTGGCGCTGCACAGCAAGAAAGAGATCGACGAGTATTTCGCGCATGTCTGGCAAACCATGCAGGCATGTATCGATCGCGGGATGAACACCGAAGGTGTACTGCCAGGCCCGCTGCGCGTGCCACGTCGTGCGTCTGCCCTGCGCCG  >  CP009273/1891624‑1891922

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑