BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	1,904,831	T→G	9.8%	L100R (CTT→CGT)	yebQ →	putative transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	1,904,831	0	T	G	9.8%	575.6 / 2.3	225	L100R (CTT→CGT)	yebQ	putative transporter
Reads supporting (aligned to +/- strand): ref base T (54/132); new base G (24/3); total (90/135)
Fisher's exact test for biased strand distribution p-value = 2.76e-09
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 233 total aligned reads displayed.

AGCCAGTTCCATCTGGGTAGTGAACGCCTATCAAATCGCCATTGTCATCTCCCTGCTCTCGTTTTCGTTTCTGGGCGATATGTTTGGCTATCGACGTATTTATAAATGCGGTCTGGTCGTTTTTCTGTTGTCTTCACTGTTCTGCGCCCTTTCTGATTCGCTGCAAATGCTCACCCTTGCGCGTGTCATACAAGGTTTCGGCGGTGCAGCGTTGATGAGCGTTAATACCGCACTTATCCGCCTGATCTATCCACAACGTTTTCTGGGTAGAGGGATGGGCATAAACTCGTTTATTGTTG  >  CP009273/1904682‑1904980

agccagTTCCATCTGGGTAGTGAACGCCTATCAAATCGCCATTGTCATCTCCCTGCTCTCGTTTTCGTTTCTGGGCGATATGTTTGGCTATCGACGTATTTATAAATGCGGTCTGGTCGTTTTTCTGTTGTCTTCACTGTTCTGCGCCCt                                                                                                                                                       <  2:4720553/150‑1 (MQ=255)

 gccagTTCCATCTGGGTAGTGAACGCCTATCAAATCGCCATTGTCATCTCCCTGCTCTCGTTTTCGTTTCTGGGCGATATGTTTGGCTATCGACGTATTTATAAATGCGGTCTGGTCGTTTTTCTGTTGTCTTCACTGTTCTGCGCCCtt                                                                                                                                                      <  1:801315/150‑1 (MQ=255)

     gTTCCATCTGGGTAGTGAACGCCTATCAAATCGCCATTGTCATCTCCCTGCTCTCGTTTTCGTTTCTGGGCGATATGTTTGGCTATCGACGTATTTATCAATGCGGTCTGGTCGTTTTTCTGGTGTCTTCAAAGTTAAGCGCCCGTTcag                                                                                                                                                  >  2:1301098/1‑148 (MQ=255)

       tCCATCTGGGTAGTGAACGCCTATCAAATCGCCATTGTCATCTCCCTGCTCTCGTTTTCGTTTCTGGGCGATATGTTTGGCTATCGACGTATTTATAAATGCGGTCTGGTCGTTTTTCTGTTGTCTTCACTGTTCTGCGCCCTTTCTGAt                                                                                                                                                <  2:4083643/150‑1 (MQ=255)

       tCCATCTGGGTAGTGAACGCCTATCAAATCGCCATTGTCATCTCCCTGCTCTCGTTTTCGTTTCTGGGCGATATGTTTGGCTATCGACGTATTTATAAATGCGGTCTGGTCGTTTTTCTGTTGTCTTCACTGTTCTGCGCCCTTTCTGAt                                                                                                                                                <  1:2842534/150‑1 (MQ=255)

         cATCTGGGTAGTGAACGCCTATCAAATCGCCATTGTCATCTCCCTGCTCTCGTTTTCGTTTCTGGGCGATATGTTTGGCTATCGACGTATTTATAAATGCGGTCTGGTCGTTTTTCTGTTTTCTTAACTGTTGTGCGCCCGTTCGGAtta                                                                                                                                              >  1:2158735/1‑149 (MQ=255)

         cATCTGGGTAGTGAACGCCTATCAAATCGCCATTGTCATCTCCCTGCTCTCGTTTTCGTTTCTGGGCGATATGTTTGGCTATCGACGTATTTATAAATGCGGTCTGGTCGTTTTTCTGTTGTCTTCACTGTTCTGCGCCCTTTCTGATTc                                                                                                                                              <  1:4082934/150‑1 (MQ=255)

         cATCTGGGTAGTGAACGCCTATCAAATCGCCATTGTCATCTCCCTGCTCTCGTTTTCGTTTCTGGGCGATATGTTTGGCTATCGACGTATTTATAAATGCGGTCTGGTCGTTGTTATGTTGTATTCACAGGTATGAGCCCGGTCAGAtta                                                                                                                                              >  1:2328958/1‑149 (MQ=255)

            cTGGGTAGTGAACGCCTATCAAATCGCCATTGTCATCTCCCTGCTCTCGTTTTCGTTTCTGGGCGATATGTTTGGCTATCGACGTATTTATAAATGCGGTCTGGTCGTTTTTCTGTTGTAGTAACAGTTGTGCGCCCTTTCTGTTTAGCt                                                                                                                                           >  1:834055/1‑150 (MQ=255)

                gTAGTGAACGCCTATCAAATCGCCATTGTCATCTCCCTGCTCTCGTTTTCGTTTCTGGGCGATATGTTTGGCTATCGACGTATTTATAAATGCGGTCTGGTCGTTTTTGTGTTGTGTTGACTGTTGGGCGCCCTGTCTGATTCGCTGaaa                                                                                                                                       >  1:4512877/1‑150 (MQ=255)

                gTAGTGAACGCCTATCAAATCGCCATTGTCATCTCCCTGCTCTCGTTTTCGTTTCTGGGCGATATGTTTGGCTATCGACGTATTTATAAATGCGGTCTGGTCGTTTTTCTGTTGTCTTCACTGTTCTGCGCCCTTTCTGATTCGCTGCaa                                                                                                                                       <  1:4042074/150‑1 (MQ=255)

                  aGTGAACGCCTATCAAATCGCCATTGTCATCTCCCTGCTCTCGTTTTCGTTTCTGGGCGATATGTTTGGCTATCGACGTATTTATAAATGCGGTCTGGTCGTTTTTCTGTTGTCTTCACTGTTCTGCGCCCTTTCTGATTCGCTGCAAAt                                                                                                                                     <  1:2073128/150‑1 (MQ=255)

                  aGTGAACGCCTATCAAATCGCCATTGTCATCTCCCTGCTCTCGTTTTCGTTTCTGGGCGATATGTTTGGCTATCGACGTATTTATAAATGCGGTCTGGTCGTTTTTCTGTTGTCTTCACTGTTCTGCGCCCTTTCTGATTCGCTGCAAAt                                                                                                                                     <  1:4592408/150‑1 (MQ=255)

                   gTGAACGCCTATCAAATCGCCATTGTCATCTCCCTGCTCTCGTTTTCGTTTCTGGGCGATATGTTTGGCTATCGACGTATTTATAAATGCGGTCTGGTCGTTTTTCTGGTGTGTTCAAAGTTGTGCGCCCGTTCTGATTAGCTGCAAATg                                                                                                                                    >  1:3267002/1‑150 (MQ=255)

                     gAACGCCTATCAAATCGCCATTGTCATCTCCCTGCTCTCGTTTTCGTTTCTGGGCGATATGTTTGGCTATCGACGTATTTATAAATGCGGTCTGGTCGTTTTTGTGTTGTGTTGACGGTTGTGCGCCCCTTGTGGTTAGGTGCaaaatca                                                                                                                                  >  1:4979523/1‑146 (MQ=255)

                     gAACGCCTATCAAATCGCCATTGTCATCTCCCTGCTCTCGTTTTCGTTTCTGGGCGATATGTTTGGCTATCGACGTATTTATAAATGCGGTCTGGTCGTGTTTGTGTTGTATTGACTGTTGTTGGGCCGTTGTGGTTCGGTGGAAATggg                                                                                                                                  >  1:2727575/1‑148 (MQ=255)

                           cTATCAAATCGCCATTGTCATCTCCCTGCTCTCGTTTTCGTTTCTGGGCGATATGTTTGGCTATCGACGTATTTATAAATGCGGTCTGGTCGTTTTTCTGTTGTCTTCACTGTTCTGCGCCCTTTCTGATTCGCTGCAAATGCTCACCCt                                                                                                                            <  1:5014488/150‑1 (MQ=255)

                            tATCAAATCGCCATTGTCATCTCCCTGCTCTCGTTTTCGTTTCTGGGCGATATGTTTGGCTATCGACGTATTTATAAATGCGGTCTGGTCGTTTTTCTGTTGTCTTCACTGTTCTGCGCCCTTTCTGATTCGCTGCAAATGCTCACCCtt                                                                                                                           <  2:1769581/150‑1 (MQ=255)

                              tCAAATCGCCATTGTCATCTCCCTGCTCTCGTTTTCGTTTCGGGGCGATATGTTTGGCTATCGACGTATTTATAAATGCGGTCTGGTCGTTTTTCTGTTGTCTTCACTGTTCTGCGCCCTTTCTGATTCGCTGCAAATGCTCACCCTTgc                                                                                                                         <  2:597959/150‑1 (MQ=255)

                              tCAAATCGCCATTGTCATCTCCCTGCTCTCGTTTTCGTGTCTGGGCGATATGTTTGGCTATCGACGTATTTATAAATGCGGTCTGGTCGTTTTTCTGTTGTCTTCACTGTTCTGCGCCCTTTCTGATTCGCTGCAAATGCTCACCCTCgc                                                                                                                         <  1:1891514/150‑1 (MQ=255)

                               cAAATCGCCATTGTCATCTCCCTGCTCTCGTTTTCGTTTCTGGGCGATATGTTTGGCTATCGACGTATTTATAAATGCGGTCTGGTCGTTTTTCTGTTGTCTTCACTGTTCTGCGCCCTTTCTGATTCGCTGCAAATGCTCACCCTTgcg                                                                                                                        <  2:459031/150‑1 (MQ=255)

                                    cGCCATTGTCATCTCCCTGCTCTCGTTTTCGTTTCTGGGCGATATGTTTGGCTATCGACGTATTTATAAATGCGGTCTGGTCGTTTTTCTGTTGTCTTCACTGTTCTGCGCCCTTTCTGATTCGCTGCAAATGCTCACCCTTGCGCgtgt                                                                                                                   <  1:2269963/150‑1 (MQ=255)

                                      ccATTGTCATCTCCCTGCTCTCGTTTTCGTTTCTGGGCGATATGTTTGGCTATCGACGTATTTATAAATGCGGTCTGGTCGTTTTTGTGTTTTGGTGAAAGTTCTGCGGCCGTTCTGATTGGCTGGAAATTCTCACCCATGcgcggggga                                                                                                                 >  1:1559957/1‑145 (MQ=255)

                                      ccATTGTCATCTCCCTGCTCTCGTTTTCGTTTCTGGGCGATATGTTTGGCTATCGACGTATTTATAAATGCGGTCTGGTCGTTTTTCTGTTGTCTTCACTGTTCTGCGCCCTTTCTGATTCGCTGCAAATGCTCACCCTTGCGCGTGTCa                                                                                                                 <  2:2451119/150‑1 (MQ=255)

                                        aTTGTCATCTCCCTGCTCTCGTTTTCGTTTCTGGGCGATATGTTTGGCTATCGACGTATTTATAAATGCGGTCTGGTCGTGTTTATGTTGTGTTCAAGGGTATGCGCCCGTTCTGATTCGCGGCACAAGCTCACCCCTGCGCGTGGCaga                                                                                                               >  1:4147059/1‑148 (MQ=255)

                                          tGTCATCTCCCTGCTCTCGTTTTCGTTTCTGGGCGATATGTTTGGCTATCGACGTATTTATAAATGCGGTCTGGTCGTTTTTCTGTTGTCTTCACTGTTCTGCGCCCTTTCTGATTCGCTGCAAATGCTCACCCTTGCGCGTGTCATACa                                                                                                             <  1:1990506/150‑1 (MQ=255)

                                             cATCTCCCTGCTCTCGTTTTCGTTTCTGGGCGATATGTTTGGCTATCGACGTATTTATAAATGCGGTCTGGTCGTTTTTCTGTTTTCTTAAAAGTTAAGCGCCCATTATGATTAGCTGCAAAATCTCACCCATGCGCGTGTAAAACAAgg                                                                                                          >  1:1715676/1‑150 (MQ=255)

                                             cATCTCCCTGCTCTCGTTTTCGTTTCTGGGCGATATGTTTGGCTATCGACGTATTTATAAATGCGGTCTGGTCGTTTTTCTGTTGTCTTCACTGTTCTGCGCCCTTTCTGATTCGCTGCAAATGCTCACCCTTGCGCGTGTCATACAAgg                                                                                                          >  2:3392694/1‑150 (MQ=255)

                                              aTCTCCCTGCTCTCGTTTTCGTTTCTGGGCGATATGTTTGGCTATCGACGTATTTATAAATGCGGTCTGGTCGTTTTTCGGGTGTCTTCACAGTTCTGCGCCCGTTCAGAATCGCTGCAAATGCTCACCCATGCGCGTGTCCTACAAGGt                                                                                                         >  2:2112078/1‑150 (MQ=255)

                                                ctcCCTGCTCTCGTTTTCGTTTCTGGGCGATATGTTTGGCTATCGACGTATTTATAAATGCGGTCTGGTCGTTTTTCTGTTGTCTTCACTGTTCTGCGCCCTTTCTGATTCGCTGCAAATGCTCACCCTTGCGCGTGTCATACAAGGttt                                                                                                       <  1:5116016/150‑1 (MQ=255)

                                                 tcCCTGCTCTCGTTTTCGTTTCTGGGCGATATGTTTGGCTATCGACGTATTTATAAATGCGGTCTGGTCGTTTTTCTGTTGTCTTCACTGTTATGCGCCCAGTCTGATTAGCTGCAAATTCTCACCCTTGCGCGTGTCATACAAGGttta                                                                                                      >  2:1613033/1‑149 (MQ=255)

                                                        tctcGTTTTCGTTTCTGGGCGATATGTTTGGCTATCGACGTATTTATAAATGCGGTCTGGTCGTTTTTCTGTTGTCTTCACTGTTCTGCGCCCTTTCTGATTCGCTGCAAATGCTCACCCTTGCGCGTGTCATACAAGGTTTCGGCGGTg                                                                                               <  2:3183102/150‑1 (MQ=255)

                                                        tctcGTTTTCGTTTCTGGGCGATATGTTTGGCTATCGACGTATTTATAAATGCGGTCTGGTCGTGTTTCTGTTTTATTAAAAGTTATTCGCCCATTCTGATTAGCAGCAAAATCTCACCCAAGCGCGTGTCATAAAACGTTTAGGCGGTg                                                                                               >  1:4426138/1‑150 (MQ=255)

                                                          tcgttttcgtttCTGGGCGATATGTTTGGCTATCGACGTATTTATAAATGCGGTCTGGTCGTTTTTCTGTTGTCTTCACTGTTCTGCGCCCTTTCTGATTCGCTGCAAATGCTCACCCTTGCGCGTGTCATACAAGGTTTCGGCGGTGCa                                                                                             <  2:2083636/150‑1 (MQ=255)

                                                            gttttcgtttCTGGGCGATATGTTTGGCTATCGACGTATTTATAAATGCGGTCTGGTCGTTTTTCTGGTGTCTTCACTGTTCTGCGCCCTTTCTGATTCGCTGCAAATGCTCACCCTTGCGCGTGTCATACAAGGTTTCGGCGGTGCAGc                                                                                           <  2:704136/150‑1 (MQ=255)

                                                              tttcgtttCTGGGCGATATGTTTGGCTATCGACGTATTTATAAATGCGGTCTGGTCGTTTTTCTGTTGTCTTCACTGTTCTGCGCCCTTTCTGATTCGCTGCAAATGCTCACCCTTGCGCGTGTCATACAAGGTTTCGGCGGTGCAGCGt                                                                                         <  1:5175539/150‑1 (MQ=255)

                                                               ttcgtttCTGGGCGATATGTTTGGCTATCGACGTATTTATAAATGCGGTCTGGTCGTTTTTCTGTTGTCTTCACTGTTCTGCGCCCTTTCTGATTCGCTGCAAATGCTCACCCTTGCGCGTGTCATACAAGGTTTCGGCGGTGCAGCGtt                                                                                        <  1:462304/150‑1 (MQ=255)

                                                                tcgtttCTGGGCGATATGTTTGGCTATCGACGTATTTATAAATGCGGTCTGGTCGTTTTTCTGTTGTCTTCACTGTTCTGCGCCCTTTCTGATTCGCTGCAAATGCTCACCCTTGCGCGTGTCATACAAGGTTTCGGCGGTGCAGCGTtg                                                                                       <  1:2867746/150‑1 (MQ=255)

                                                                tcgtttCTGGGCGATATGTTTGGCTATCGACGTATTTATAAATGCGGTCTGGTCGTTTTTCTGTTGTCTTCACTGTTCTGCGCCCTTTCTGAGTCGCTGCAAATGCTCACCCTTGCGCGTGTCATACAAGGTTTCGGCGGTGCAGCGTtg                                                                                       <  1:4129580/150‑1 (MQ=255)

                                                                tcgtttCTGGGCGATATGTTTGGCTATCGACGTATTTATAAATGCGGTCTGGTCGTTTTTCTGTTGTCTTCACTGTTCTGCGCCCTGTCTGATTCGCTGCAAATGCTCACCCTTGCGCGTGTCATACAAGGTTTCGGCGGTGCAGCGTtg                                                                                       <  1:4810328/150‑1 (MQ=255)

                                                                tcgtttCTGGGCGATATGTTTGGCTATCGACGTATTTATAAATGCGGTCTGGTCGTTTTTATGTTGTCTTCACTTTTCTGCGCCCTTTCTGATTCGCTGCAAATTCTCACCCTTGCGCGTGTCATACAAGGTTTCGGCGGTGCAGCGTtg                                                                                       >  2:1611291/1‑150 (MQ=255)

                                                                  gtttCTGGGCGATATGTTTGGCTATCGACTTATTTATAAATGCGGTCTGGTCGTTTTTCTGTTGTCTTCACTGTTCTGCGCCCTTTCTGATTCGCTGCAAATGCTCACCCTTGCGCGTGTCATACAAGGTTTCGGCGGTGCAGCGTtgat                                                                                     <  2:1665173/150‑1 (MQ=255)

                                                                  gtttCTGGGCGATATGTTTGGCTATCGACGTATTTATAAATGCGGTCTGGTCGTTTTTCTGTTGTCTTCACTGTTCTGCGCCCTTTCTGATTCGCTGCAAATGCTCACCCTTGCGCGTGTCATACAAGGTTTCGGCGGTGCAGCGTtgat                                                                                     <  2:4007360/150‑1 (MQ=255)

                                                                        gggCGATATGTTTGGCTATCGCCGTATTTATAAATGCGGTCTGGTCGTTTTTCTGTTGTCTTCACTGTTCTGCGCCCTTTCTGATTCGCTGCAAATGCTCACCCTTGCGCGTGTCATACAAGGTTTCGGCGGTGCAGCGTTGATGAGCGt                                                                               <  2:3880595/150‑1 (MQ=255)

                                                                          gCGATATGTTTGGCTATCGACGTATTTATAAATGCGGTCTGGTCGTTTTTCTGTTGTCTTCACTGTTCTGCGCCCTTTCTGATTCGCTGCAAATGCTCAGCCTTGCGCGTGTCATACAAGGTTTCGGCGGTGCAGCGTTGATGAGCGTTa                                                                             <  1:1867729/150‑1 (MQ=255)

                                                                              tatGTTTGGCTATCGACGTATTTATAAATGCGGTCTGGTCGTTTTTCTGTTGTCTTCACTGTTCTGCGCCCTTTCTGATTCGCTGCAAATGCTCACCCTTGCGCGTGTCATACAAGGTTTCGGCGGTGCAGCGTTGATGAGCGTTAATAc                                                                         <  1:2573049/150‑1 (MQ=255)

                                                                               atGTTTGGCTATCGACGTATTTATAAATGCGGTCTGGTCGTTTTTGTGGTGTGTTCACAGTTGTGCGCCCAGTCTGATTCGCTGCAAATGCTCACCCAAGCGCGTGTCCTAAAAGGTTTAGGCGGTGGAGCGTTGATTAGAGGTAATaac                                                                        >  2:439857/1‑148 (MQ=255)

                                                                               atGTTTGGCTATCGACGTATTTATAAATGCGGTCTGGTCGTGTTTATTTTTTATTAACAGTTCTGCGCCCATTCTGATTAGCTGCAAATTCTCACCCATTCGCGTGTCAAAAAAGGTTTAGGCGGTGCCGCGTTGATGATCGTTAATaac                                                                        >  1:1238821/1‑148 (MQ=255)

                                                                                  tttGGCTATCGACGTATTTATAAATGCGGTCTGGTCGTTTTTCTGTTGTCTTCACTGTTCTGCGCCCTTTCTGATTCGCTGCAAATGCTCACCCTTGCGCGTGTCATACAAGGTTTCGGCGGTGCAGCGTTGATGAGCGTTAATACCGCa                                                                     <  1:3623453/150‑1 (MQ=255)

                                                                                   ttGGCTATCGACGTATTTATAAATGCGGTCTGGTCGTTTTTCTGTTGTCTTCACTGTTCTGCGCCCTTTCTGATTCGCTGCAAATGCTCACCCTTGCGCGTGTCATACAAGGTTTCGGCGGTGCAGCGTTGATGAGCGTTAATACCGCAc                                                                    <  1:771525/150‑1 (MQ=255)

                                                                                    tGGCTATCGACGTATTTATAAATGCGGTCTGGTCGTTTTTCTGTTGTCTTCACTGTTCTGCGCCCTTTCTGATTCGCTGCAAATGCTCACCCTTGCGCGTGTCATACAAGGTTTCGGCGGTGCAGCGTTGATGAGCGTTAATACCGCACt                                                                   <  2:820463/150‑1 (MQ=255)

                                                                                    tGGCTATCGACGTATTTATAAATGCGGTCTGGTCGTTTTTCTGTTGTCTTCACTGTTCTGCGCCCTTTCTGATTCGCTGCAAATGCTCACCCATGCGCGTGTCATACAAGGTTTCGGCGGTGCAGCGTTGATGAGCGGTAATACCGCACt                                                                   >  2:2888986/1‑150 (MQ=255)

                                                                                     ggCTATCGACGTATTTATAAATGCGGTCTGGTCGTTTTTCTGTTGTCTTCACTGTTCTGCGCCCTTTCTGATTCGCTGCAAATGCTCACCCTTGCGCGTGTCATACAAGGTTTCGGCGGTGCAGCGTTGATGAGCGTTAATACCGCACtt                                                                  <  2:5027729/150‑1 (MQ=255)

                                                                                       cTATCGACGTATTTATAAATGCGGTCTGGTCGTGTTTGTTTTGTCTTAAAAGTTAAGCGCCCAGTATGATTCGCAGCAAAATCAAACCCATGAGCGTGTCAAACAAAGGTTAGGAGGTGCAGCCTTTTAGAGCGTTAAAACCGCCCatat                                                                >  2:947825/1‑150 (MQ=255)

                                                                                             aCGTATTTATAAATGCGGTCTGGTCGTTTTTCTGTTGTCTTCACTGTTCTGCGCCCTTTCTGATTCGCTGCAAATGCTCACCCTTGCGCGTGTCATACAAGGTTTCGGCGGTGCAGCGTTGATGAGCGTTAATACCGCACTTATCCGCCt                                                          <  2:653858/150‑1 (MQ=255)

                                                                                              cGTATTTATAAATGCGGTCTGGTCGTTTTTCTGTTGTCTTCACTGTTCTGCGCCCTTTATGATTAGCTGCAAATTCTAACCCTTGCGCGTGTCATACAAGGTTTAGGCGGTGCAGCGTTGATGAGCGGTAATACCGCACATATACGCCTg                                                         >  2:1249978/1‑150 (MQ=255)

                                                                                               gTATTTATAAATGCGGTCTGGTCGTTTTTCTGTTGTCTTCACTGTTCTGCGCCCTTTCTGATTCGCTGCAAATTCTCACCCGTGCGCGTGTCATACAAGGTTTCGGCGGTGCAGCGTTGATGAGCGTTAATACCGCACTTATACGCCTGa                                                        >  2:5103984/1‑150 (MQ=255)

                                                                                                  tttATAAATGCGGTCTGGTCGTTTTTCTGTTGTCTTCACTGTTCTGCGCCCTTTCTGATTCGCTGCAAATGCTCACCCTTGCGCGTGTCATACAAGGTTTCGGCGGTGCAGCGTTGATGAGCGTTAATACCGCACTTATCCGCCTGATCt                                                     <  1:3936673/150‑1 (MQ=255)

                                                                                                   ttATAAATGCGGTCTGGTCGTTTTTCTGTTGTCTTCACTGTTCTGCGCCCTTTCTGATTCGCTGCAAATGCTCACCCTTGCGCGTGTCATACAAGGTTTCGGCGGTGCAGCGTTGATGAGCGTTAATACCGCACTTATCCGCCTGATCTa                                                    <  2:321814/150‑1 (MQ=255)

                                                                                                   ttATAAATGCGGTCTGGTCGTTTTTCTGTTGTCTTCACTGTTCTGCGCCCTTTCTGATTCGCTGCAAATGCTCACCCTTGCGCGTGTCATACAAGGTTTCGGCGGTGCAGCGTTGATGAGCGTTAATACCGCACTTATCCGCCTGATCTa                                                    <  2:3393224/150‑1 (MQ=255)

                                                                                                   ttATAAATGCGGTCTGGTCGTTTTTCTGTTGTCTTCACTGTTCTGCGCCCTTTCTGATTCGCTGCAAATGCTCACCCTTGCGCGTGTCATACAAGGTTTCGGCGGTGCAGCGTTGATGAGCGTTAATACCGCACTTATCCGCCTGATCTa                                                    <  1:724469/150‑1 (MQ=255)

                                                                                                     acacacgCGGTCTGGTCGTTTTTCGGGTCTCTTCACTGTGCTGCGGCCTTTCTGCTTCGCGGCAACTGCTCCCCCGTGCGCGTGTCATACCAGGTTTCGGCGGTGCATCGTTGATGAGCGTTAATACCGCACTTATCCGCCTGATCTATc                                                  <  2:4352660/144‑1 (MQ=255)

                                                                                                           gCGGTCTGGTCGTTTTTCTGTTGTCTTCACTGTTCTGCGCCCTTTCTGATTCGCTGCAAATGCTCACCCTTGCGCGTGTCATACAAGGTTTCGGCGGTGCAGCGTTGATGAGCGTTAATACCGCACTTATCCGCCTGATCTATCCACAAc                                            <  2:3806028/150‑1 (MQ=255)

                                                                                                              gTCTGGTCGTTTTTCTGTTGTCTTCACTGTTCTGCGCCCTTTCTGATTCGCTGCAAATGCTCACCCTTGCGCGTGTCATACAAGGTTTCGGCGGTGCAGCGTTGATGAGCGTTAATACCGCACTTATCCGCCTGATCTATCCACAACGtt                                         <  2:171040/150‑1 (MQ=255)

                                                                                                                cTGGTCGTTTTTCTGTTGTCTTCACTGTTCTGCGCCCTTTCTGATTCGCTGCAAATGCTCACCCTTGCGCGTGTCATACAAGGTTTCGGCGGTGCAGCGTTGATGAGCGTTAATACCGCACTTATCCGCCTGATCTATCCACAACGtttt                                       <  1:1180107/150‑1 (MQ=255)

                                                                                                                cTGGTCGTTTTTCTGTTGTCTTCACTGTTCTGCGCCCTTTCTGATTCGCTGCAAATGCTCACCCTTGCGCGTGTCATACAAGGTTTCGGCGGTGCAGCGTTGATGAGCGTTAATACCGCACTTATCCGCCTGATCTATCCACAACGtttt                                       <  1:1180128/150‑1 (MQ=255)

                                                                                                                cTGGTCGTTTTTCTGTTGTCTTCACTGTTCTGCGCCCTTTCTGATTCGCTGCAAATGCTCACCCTTGCGCGTGTCATACAAGGTTTCGGCGGTGCAGCGTTGATGAGCGTTAATACCGCACTTATCCGCCTGATCTATCCACAACGtttt                                       <  1:2945555/150‑1 (MQ=255)

                                                                                                                 tGGTCGTTTTTCTGTTGTCTTCACTGTTCTGCGCCCTTTCTGATTCGCTGCAAATGCTCACCCTTGCGCGTGTCATACAAGGTTTCGGCGGTGCAGCGTTGATGAGCGTTAATACCGCACTTATCCGCCTGATCTATCCACAACGTTTTc                                      <  1:1463824/150‑1 (MQ=255)

                                                                                                                 tGGTCGTTTTTCTGTTGTCTTCACTGTTCTGCGCCCTTTCTGATTCGCTGCAAATGCTCACCCTTGCGCGTGTCATACAAGGTTTCGGCGGTGCAGCGTTGATGAGCGTTAATACCGCACTTATCCGCCTGATCTATCCACAACGTTTTc                                      <  2:3707599/150‑1 (MQ=255)

                                                                                                                 tGGTCGTTTTTCTGGTGTCTTCACTGTTCTGCGCCCTTTCTGATTCGCTGCAAATGCTCACCCTTGCGCGTGTCATACAAGGTTTCGGCGGTGCAGCGTTGATGAGCGTTAATACCGCACTTATCCGCCTGATCTATCCACAACGTTTTc                                      <  2:5169745/150‑1 (MQ=255)

                                                                                                                     cGTTTTTCTGTTGTCTTCACTGTTCTGCGCCCTTTCTGATTCGCTGCAAATGCTCACCCTTGCGCGTGTCATACAAGGTTTCGGCGGTGCAGCGTTGATGAGCGTTAATACCGCACTTATCCGCCTGATCTATCCACAACGTTTTCTggg                                  <  1:3392694/150‑1 (MQ=255)

                                                                                                                     cGTTTTTCTGTTGTCTTCACTGTTCTGCGCCCTTTCTGATTCGCTGCAAATGCTCACCCTTGCGCGTGTCATACAAGGTTTCGGCGGTGCAGCGTTGATGAGCGTTAATACCGCACTTATCCGCCTGATCTATCCACAACGTTTTCTggg                                  <  1:3392835/150‑1 (MQ=255)

                                                                                                                     cGTTTTTCTGTTGTCTTCACTGTTCTGCGCCCTTTCTGATTCGCTGCAAATGCTCACCCTTGCGCGTGTCATACAAGGTTTCGGCGGTGCAGCGTTGATGAGCGTTAATACCGCACTTATCCGCCTGATCTATCCACAACGTTTTCTggg                                  >  2:4291069/1‑150 (MQ=255)

                                                                                                                           tCTGTTGTCTTCACTGTTCTGCGCCCTTTCTGATTCGCTGCAAATGCTCACCCTTGCGCGTGTCATACAAGGTTTCGGCGGTGCAGCGTTGATGAGCGTTAATACCGCACTTATCCGCCTGATCTATCCACAACGTTTTCTGGGTAGAgg                            <  1:102766/150‑1 (MQ=255)

                                                                                                                           tCTGTTGTCTTCACTGTTCTGCGCCCTTTCTGATTCGCTGCAAATGCTCACCCTTGCGCGTGTCATACAAGGTTTCGGCGGTGCAGCGTTGATGAGCGTTAATACCGCACTTATCCGCCTGATCTATCCACAACGTTTTCTGGGTAGAgg                            <  2:4678329/150‑1 (MQ=255)

                                                                                                                           tCTGTTGTCTTCACTGTTCTGCGCCCTTTCTGATTCGCTGCAAATGCTCACCCTTGCGCGTGTCATACAAGGTTTCGGCGGTGCAGCGTTGATGAGCGTTAATACCGCACTTATCCGCCTGATCTATCCACAACGTTTTCTGGGTAGAgg                            <  1:3556098/150‑1 (MQ=255)

                                                                                                                            cTGTTGTCTTCACTGTTCTGCGCCCTTTCTGATTCGCTGCAAATGCTCACCCTTGCGCGTGTCATACAAGGTTTCGGCGGTGCAGCGTTGATGAGCGTTAATACCGCACTTATCCGCCTGATCTATCCACAACGTTTTCTGGGTAGAggg                           <  2:4946986/150‑1 (MQ=255)

                                                                                                                            cTGTTGTCTTCACTGTTCTGCGCCCTTTCTGATTCGCTGCAAATGCTCACCCTTGCGCGTGTCATACAAGGTTTCGGCGGTGCAGCGTTGATGAGCGTTAATACCGCACTTATCCGCCTGATCTATCCACAACGTTTTCTGGGTAGAggg                           <  2:3267002/150‑1 (MQ=255)

                                                                                                                              gttgtCTTCACTGTTCTGCGCCCTTTCTGATTCGCTGCAAATGCTCACCCTTGCGCGTGTCATACAAGGTTTCGGCGGTGCAGCGTTGATGAGCGTTAATACCGCACTTATCCGCCTGATCTATCCACAACGTTTTCTGGGTAGAGGGAt                         >  1:4917757/1‑150 (MQ=255)

                                                                                                                              gttgtCTTCACTGTTCTGCGCCCTTTCTGATTCGCTGCAAATGCTCACCCTTGCGCGTGTCATACAAGGTTTCGGCGGTGCAGCGTTGATGAGCGTTAATACCGCACTTATCCGCCTGATCTATCCACAACGTTTTCTGGGTAGAGGGAt                         <  1:430807/150‑1 (MQ=255)

                                                                                                                              gttgtCTTCACTGTTCTGCGCCCTTTCTGATTCGCTGCAAATGCTCACCCTTGCGCGTGTCATACAAGGTTTCGGCGGTGCAGCGTTGATGAGCGTTAATACCGCACTTATCCGCCTGATCTATCCACAACGTTTTCTGGGTAGAGGGAt                         >  2:3619078/1‑150 (MQ=255)

                                                                                                                               ttgtCTTCACTGTTCTGCGCCCTTTCTGATTCGCTGCAAATGCTCACCCTTGCGCGTGTCATACAAGGTTTCGGCGGTGCAGCGTTGATGAGCGTTAATACCGCACTTATCCGCCTGATCTATCCACAACGTTTTCTGGGTAGAGGGATg                        <  2:2026974/150‑1 (MQ=255)

                                                                                                                               ttgtCTTCACTGTTCTGCGCCCTTTCTGAGTCGCTGCAAATGCTCACCCTTGCGCGTGTCATACAAGGTTTCGGCGGTGCAGCGTTGATGAGCGTTAATACCGCACTTATCCGCCTGATCTATCCACAACGTTTTCTGGGTAGAGGGATg                        <  2:5013791/150‑1 (MQ=255)

                                                                                                                                tgtCTTCACTGTTCTGCGCCCTGTCTGATTCGCTGCAAATGCTCACCCTTGCGCGTGGCATACAAGGTTTCGGCGGTGCAGCGTTGATGAGCGTTAATACCGCACTTATCCGCCTGATCTATCCACAACGATTTCTGGGTAGAGGGATgg                       >  2:2803300/1‑150 (MQ=255)

                                                                                                                                 gtCTTCACTGTTCTGCGCCCGGTCTGATTCGCTGCAAATGCTCACCCTTGCGCGTGTCATACAAGGTTTCGGCGGTGCAGCGTTGATGAGCGTTAATACCGCACTTATCCGCCTGATCTATCCACAACGTTTTCTGGGTAGAGGGATggg                      <  1:4568725/150‑1 (MQ=255)

                                                                                                                                    ttCACTGTTCTGCGCCCTTTCTGATTCGCTGCAAATGCTCACCCTTGCGCGTGTCATACAAGGTTTCGGCGGTGCAGCGTTGATGAGCGTTAATACCGCACTTATCCGCCTGATCTATCCACAACGTTTTCTGGGTAGAGGGATGGGCAt                   >  1:2251786/1‑150 (MQ=255)

                                                                                                                                    ttCACTGTTCTGCGCCCTTTCTGATTCGCTGCAAATGCTCACCCTTGCGCGTGTCATACAAGGTTTCGGCGGTGCAGCGTTGATGAGCGTTAATACCGCACTTATCCGCCTGATCTATCCACAACGTTTTCTGGGTAGAGGGATGGGCAt                   >  1:3957154/1‑150 (MQ=255)

                                                                                                                                     tCACTGTTCTGCGCCCTTTCTGATTCGCTGCAAATGCTCACCCTTGCGCGTGTCATACAAGGTTTCGGCGGTGCAGCGTTGATGAGCGTTAATACCGCACTTATCCGCCTGATCTATCCACAACGTTTTCTGGGTAGAGGGATGGGCATa                  <  2:4067671/150‑1 (MQ=255)

                                                                                                                                        cTGTTCTGCGCCCTTTCTGATTCGCTGCAAATGCTCACCCTTGCGCGTGTCATACAAGGTTTCGGCGGTGCAGCGTTGATGAGCGTTAATACCGCACTTATCCGCCTGATCTATCCACAACGTTTTCTGGGTAGAGGGATGGGCATAAAc               <  2:1124278/150‑1 (MQ=255)

                                                                                                                                        cTGTTCTGCGCCCTTTCTGATTCGCTGCAAATGCTCACCCTTGCGCGTGTCATACAAGGTTTCGGCGGTGCAGCGTTGATGAGCGTTAATACCGCACTTATCCGCCTGATCTATCCACAACGTTTTCTGGGTAGAGGGATGGGCATAAAc               <  2:2929652/150‑1 (MQ=255)

                                                                                                                                           ttCTGCGCCCTTTCTGATTCGCTGCAAATGCTCACCCTTGCGCGTGTCATACAAGGTTTCGGCGGTGCAGCGTTGATGAGCGTTAATACCGCACTTATCCGCCTGATCTATCCACACCGTTTTCTGGGTAGAGGGATGGGCATAAACTCg            <  2:1268439/150‑1 (MQ=255)

                                                                                                                                            tCTGCGCCCTTTCTGATTCGCTGCAAATGCTCACCCTTGCGCGTGTCATACAAGGTTTCGGCGGTGCAGCGTTGATGAGCGTTAATACCGCACTTATCCGCCTGATCTATCCACAACGTTTTCTGGGTAGAGGGATGGGCATAAACTCGt           >  1:87767/1‑150 (MQ=255)

                                                                                                                                              tGCGCCCTTTCTGATTCGCTGCAAATGCTCACCCTTGCGCGTGTCATACAAGGTTTCGGCGGTGCAGCGTTGATGAGCGTTAATACCGCACTTATCCGCCTGATCTATCCACAACGTTTTCTGGGTAGAGGGATGGGCATAAACTCGttt         <  1:318953/150‑1 (MQ=255)

                                                                                                                                               gcgcCCTTTCTGATTCGCTGCAAATGCTCACCCTTGCGCGTGTCATACAAGGTTTCGGCGGTGCAGCGTTGATGAGCGTTAATACCGCACTTATCCGCCTGATCTATCCACAACGTTTTCTGGGTAGAGGGATGGGCATAAACTCGTTTa        >  2:531420/1‑150 (MQ=255)

                                                                                                                                               gcgcCCTTTCTGATTCGCTGCAAATGCTCACCCTTGCGCGTGTCATACAAGGTTTCGGCGGTGCAGCGTTGATGAGCGTTAATACCGCACTTATCCGCCTGATCTATCCACAACGTTTTCTGGGTAGAGGGATGGGCATAAACTCGTTTa        >  2:850182/1‑150 (MQ=255)

                                                                                                                                                 gcCCTTTCTGATTCGCTGCAAATGCTCACCCTTGCGCGTGTCATACAAGGTTTCGGCGGTGCAGCGTTGATGAGCGTTAATACCGCACTTATCCGCCTGATCTATCCACAACGTTTTCTGGGTAGAGGGATGGGCATAAACTCGTTTAtt      <  1:3446690/150‑1 (MQ=255)

                                                                                                                                                 gcCCGTTCTGAGTCGCTGCACATGCTCACCCTTGCGCGTGTCATACAAGGTTTCGGCGGTGCAGCGTTGATGAGCGTTAATACCGCACTTATCCGCCTGATCTATCCACAACGTTTTCTGGGTAGAGGGATGGGCATAAACTCGTTTAtt      <  1:3608093/150‑1 (MQ=255)

                                                                                                                                                  cccTTTCTGATTCGCTGCAAATGCTCACCCTTGCGCGTGTCATACAAGGTTTCGGCGGTGCAGCGTTGATGAGCGTTAATACCGCACTTATCCGCCTGATCTATCCACAACGTTTTCTGGGTAGAGGGATGGGCATAAACTCGTTTAttg     >  2:2981190/1‑150 (MQ=255)

                                                                                                                                                   ccTTTCTGATTCGCTGCAAATGCTCACCCTTGCGCGTGTCATACAAGGTTTCGGCGGTGCAGCGTTGATGAGCGTTAATACCGCACTTATCCGCCTGATCTATCCACAACGTTTTCTGGGTAGAGGGATGGGCATAAACTCGTTTAttgt    >  2:1452329/1‑150 (MQ=255)

                                                                                                                                                     tttCTGAGTCGCTGCAAATGCTCACCCTTGCGCGTGTCATACAAGGTTTCGGCGGTGCAGCGTTGATGAGCGTTAATACCGCACTTATCCGCCTGATCTATCCACAACGTTTTCTGGGTAGAGGGATGGGCATAAACTCGTTTAttgttg  <  2:3466669/150‑1 (MQ=255)

AGCCAGTTCCATCTGGGTAGTGAACGCCTATCAAATCGCCATTGTCATCTCCCTGCTCTCGTTTTCGTTTCTGGGCGATATGTTTGGCTATCGACGTATTTATAAATGCGGTCTGGTCGTTTTTCTGTTGTCTTCACTGTTCTGCGCCCTTTCTGATTCGCTGCAAATGCTCACCCTTGCGCGTGTCATACAAGGTTTCGGCGGTGCAGCGTTGATGAGCGTTAATACCGCACTTATCCGCCTGATCTATCCACAACGTTTTCTGGGTAGAGGGATGGGCATAAACTCGTTTATTGTTG  >  CP009273/1904682‑1904980

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`