Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 118,873 G→T 100% D99Y (GAC→TAC)  pdhR → pyruvate dehydrogenase complex repressor; autorepressor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273118,8730GT97.7% 783.8 / 4.0 219D99Y (GAC→TAC) pdhRpyruvate dehydrogenase complex repressor; autorepressor
Reads supporting (aligned to +/- strand):  ref base G (2/3);  new base T (104/110);  total (106/113)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 219 total aligned reads displayed.

CTTGCGTGAGGCGATTCAACGTCTCGAAGCGAAGGGCTTGTTGCTTCGTCGCCAGGGTGGCGGCACTTTTGTCCAGAGCAGCCTATGGCAAAGCTTCAGCGATCCGCTGGTGGAGCTGCTCTCCGACCATCCTGAGTCACAGTATGACTTGCTCGAAACACGACACGCCCTGGAAGGTATCGCCGCTTATTACGCCGCGCTGCGTAGTACCGATGAAGACAAGGAACGCATCCGTGAACTCCACCACGCCATAGAGCTGGCGCAGCAGTCTGGCGATCTGGACGCGGAATCA  >  CP009273/118728‑119019
                                                                                                                                                 |                                                                                                                                                  
ctTGCGTGAGGCGATTCAACGTCTCGAAGCGAAGGGCTTGTTGCTTCGTCGCCAGGGTGGCGGCACTTTTGTCCAGAGCAGCCTATGGCAAAGCTTCAGCGATCCGCTGGTGGAGCTGCTCTCCGACCATCCTGAGTCACAGTATTACtt                                                                                                                                                >  2:1651107/1‑150 (MQ=255)
  tGCGTGAGGCGATTCAACGTCTCGAAGCGAAGGGCTTGTTGCTTCGTCGCCAGGGTGGCGGCACTTTTGTCCAGAGCAGCCTATGGCAAAGCTTCAGCGATCCGCTGGTGGAGCTGCTCTCCGACCATCCTGAGTCACAGTATTACTTGc                                                                                                                                              <  2:1335759/150‑1 (MQ=255)
    cGTGAGGCGATTCAACGTCTCGAAGCGAAGGGCTTGTTGCTTCGTCGCCAGGGTGGCGGCACTTTTGTCCAGAGCAGCCTATGGCAAAGCTTCAGCGATCCGCTGGTGGAGCTGCTCTCCGACCATCCTGAGTCACAGTATTACTTGCTc                                                                                                                                            >  1:3417020/1‑150 (MQ=255)
    cGTGAGGCGATTCAACGTCTCGAAGCGAAGGGCTTGTTGCTTCGTCGCCAGGGTGGCGGCACTTTTGTCCAGAGCAGCCTATGGCAAAGCTTCAGCGATCCGCTGGTGGAGCTGCTCTCCGACCATCCTGAGTCACAGTATTACTTGCTc                                                                                                                                            <  2:2644344/150‑1 (MQ=255)
      tGAGGCGATTCAACGTCTCGAAGCGAAGGGCTTGTTGCTTCGTCGCCAGGGTGGCGGCACTTTTGTCCAGAGCAGCCTATGGCAAAGCTTCAGCGATCCGCTGGTGGAGCTGCTCTCCGACCATCCTGAGTCACAGTATTACTTGCTCGa                                                                                                                                          >  2:4917375/1‑150 (MQ=255)
      tGAGGCGATTCAACGTCTCGAAGCGAAGGGCTTGTTGCTTCGTCGCCAGGGTGGCGGCACTTTTGTCCAGAGCAGCCTATGGCAAAGCTTCAGCGATCCGCTGGTGGAGCTGCTCTCCGACCATCCTGAGTCACAGTATTACTTGCTCGa                                                                                                                                          >  1:2430990/1‑150 (MQ=255)
       gAGGCGATTCAACGTCTCGAAGCGAAGGGCTTGTTGCTTCGTCGCCAGGGTGGCGGCACTTTTGTCCAGAGCAGCCTATGGCAAAGCTTCAGCGATCCGCTGGTGGAGCTGCTCTCCGACCATCCTGAGTCACAGTATTACTTGCTCGaa                                                                                                                                         >  1:594894/1‑150 (MQ=255)
         ggCGATTCAACGTCTCGAAGCGAAGGGCTTGTTGCTTCGTCGCCAGGGTGGCGGCACTTTTGTCCAGAGCAGCCTATGGCAAAGCTTCAGCGATCCGCTGGTGGAGCTGCTCTCCGACCATCCTGAGTCACAGTATTACTTGCTCGAAac                                                                                                                                       <  1:4479019/150‑1 (MQ=255)
         ggCGATTCAACGTCTCGAAGCGAAGGGCTTGTTGCTTCGTCGCCAGGGTGGCGGCACTTTTGTCCAGAGCAGCCTATGGCAAAGCTTCAGCGATCCGCTGGTGGAGCTGCTCTCCGACCATCCTGAGTCACAGTATTACTTGCTCGAAac                                                                                                                                       <  1:2949756/150‑1 (MQ=255)
         ggCGATTCAACGTCTCGAAGCGAAGGGCTTGTTGCTTCGTCGCCAGGGTGGCGGCACTTTTGTCCAGAGCAGCCTATGGCAAAGCTTCAGCGATCCGCTGGTGGAGCTGCTCTCCGACCATCCTGAGTCACAGTATTACTTGCTCGAAac                                                                                                                                       <  1:773772/150‑1 (MQ=255)
         ggCGACTCAACGTCTCGAAGCGAAGGGCTTGTTGCTTCGTCGCCAGGGTGGCGGCACTTTTGTCCAGAGCAGCCTATGGCAAAGCTTCAGCGATCCGCTGGTGGAGCTGCTCTCCGACCATCCTGAGTCACAGTATTACTTGCTCGAAac                                                                                                                                       <  1:3946285/150‑1 (MQ=255)
          gCGATTCAACGTCTCGAAGCGAAGGGCTTGTTGCTTCGTCGCCAGGGTGGCGGCACTTTTGTCCAGAGCAGCCTATGGCAAAGCTTCAGCGATCCGCTGGTGGAGCTGCTCTCCGACCATCCTGAGTCACAGTATTACTTGCTCGAAaca                                                                                                                                      <  1:4813150/150‑1 (MQ=255)
           cGATTCAACGTCTCGAAGCGAAGGGCTTGTTGCTTCGTCGCCAGGGTGGCGGCACTTTTGTCCAGAGCAGCCTATGGCAAAGCTTCAGCGATCCGCTGGTGGAGCTGCTCTCCGACCATCCTGAGTCACAGTATTACTTGCTCGAAacac                                                                                                                                     <  1:1954290/150‑1 (MQ=255)
             aTTCAACGTCTCGAAGCGAAGGGCTTGTTGCTTCGTCGCCAGGGTGGCGGCACTTTTGTCCAGAGCAGCCTATGGCAAAGCTTCAGCGATCCGCTGGTGGAGCTGCTCTCCGACCATCCTGAGTCACAGTATTACTTGCTCGAAacacga                                                                                                                                   >  2:2302820/1‑150 (MQ=255)
               tCAACGTCTCGAAGCGAAGGGCTTGTTGCTTCGTCGCCAGGGTGGCGGCACTTTTGTCCAGAGCAGCCTATGGCAAAGCTTCAGCGATCCGCTGGTGGAGCTGCTCTCCGACCATCCTGAGTCACAGTATTACTTGCTCGAAacacgaca                                                                                                                                 <  1:3794156/150‑1 (MQ=255)
                cAACGTCTCGAAGCGAAGGGCTTGTTGCTTCGTCGCCAGGGTGGCGGCACTTTTGTCCAGAGCAGCCTATGGCAAAGCTTCAGCGATCCGCTGGTGGAGCTGCTCTCCGACCATCCTGAGTCACAGTATTACTTGCTCGAAacacgacac                                                                                                                                <  2:3722272/150‑1 (MQ=255)
                    gTCTCGAAGCGAAGGGCTTGTTGCTTCGTCGCCAGGGTGGCGGCACTTTTGTCCAGAGCAGCCTATGGCAAAGCTTCAGCGATCCTCTGGTGGAGCTGCTCTCCGACCATCCTGAGTCACAGTATTACTTGCTCGAAACACGACACGccc                                                                                                                            >  1:2105770/1‑150 (MQ=255)
                    gTCTCGAAGCGAAGGGCTTGTTGCTTCGTCGCCAGGGTGGCGGCACTTTTGTCCAGAGCAGCCTATGGCAAAGCTTCAGCGATCCGCTGGTGGAGCTGCTCTCCGACCATCCTGAGTCACAGTATTACTTGCTCGAAACACGACACGccc                                                                                                                            >  1:4689339/1‑150 (MQ=255)
                      ctcGAAGCGAAGGGCTTGTTGCTTCGTCGCCAGGGTGGCGGCACTTTTGTCCAGAGCAGCCTATGGCAAAGCTTCAGCGATCCGCTGGTGGAGCTGCTCTCCGACCATCCTGAGTCACAGTATTACTTGCTCGAAACACGACACGCCCTg                                                                                                                          <  1:1071878/150‑1 (MQ=255)
                       tcGAAGCGAAGGGCTTGTTGCTTCGTCGCCAGGGTGGCGGCACTTTTGTCCAGAGCAGCCTATGGCAAAGCTTCAGCGATCCGCTGGTGGAGCTGCTCTCCGACCATCCTGAGTCACAGTATTACTTGCTCGAAACACGACACGCCCTgg                                                                                                                         >  1:4702257/1‑150 (MQ=255)
                        cgaagcgaagGGCTTGTTGCTTCGTCGCCAGGGTGGCGGCACTTTTGTCCAGAGCAGCCTATGGCAAAGCTTCAGCGATCCGCTGGTGGAGCTGCTCTCCGACCATCCTGAGTCACAGTATTACTTGCTCGAAACACGACACGCCCTGGa                                                                                                                        >  1:1309112/1‑150 (MQ=255)
                          aagcgaagGGCTTGTTGCTTCGTCGCCAGGGTGGCGGCACTTTTGTCCAGAGCAGCCTATGGCAAAGCTTCAGCGATCCGCTGGTGGAGCTGCTCTCCGACCATCCTGAGTCACAGTATTACTTGCTCGAAACACGACACGCCCTGGAAg                                                                                                                      >  2:3893423/1‑150 (MQ=255)
                          aagcgaagGGCTTGTTGCTTCGTCGCCAGGGTGGCGGCACTTTTGTCCAGAGCAGCCTATGGCAAAGCTTCAGCGATCCGCTGGTGGAGCTGCTCTCCGACCATCCTGAGTCACAGTATTACTTGCTCGAAACACGACACGCCCTGGAAg                                                                                                                      <  1:1432393/150‑1 (MQ=255)
                          aagcgaagGGCTTGTTGCTTAGTCGCCAGGGTGGCGGCACTTTTGTCCAGAGCAGCCTATGGCAAAGCTTCAGCGATCCGCTGGTGGAGCTGCTCTCCGACCATCCTGAGTCACAGTATTACTTGCTCGAAACACGACACGCCCTGGAAg                                                                                                                      >  2:1120937/1‑150 (MQ=255)
                           agcgaagGGCTTGTTGCTTCGTCGCCAGGGTGGCGGCACTTTTGTCCAGAGCAGCCTATGGCAAAGCTTCAGCGATCCGCTGGTGGAGCTGCTCTCCGACCATCCTGAGTCACAGTATTACTTGCTCGAAACACGACACGCCCTGGAAgg                                                                                                                     <  2:3892832/150‑1 (MQ=255)
                              gaagGGCTTGTTGCTTCGTCGCCAGGGTGGCGGCACTTTTGTCCAGAGCAGCCTATGGCAAAGCTTCAGCGATCCGCTGGTGGAGCTGCTCTCCGACCATCCTGAGTCACAGTATTACTTGCTCGAAACACGACACGCCCTGGAAGGTAt                                                                                                                  >  1:4939527/1‑150 (MQ=255)
                                  ggCTTGTTGCTTCGTCGCCAGGGTGGCGGCACTTTTGTCCAGAGCAGCCTATGGCAAAGCTTCAGCGATCCGCTGGTGGAGCTGCTCTCCGACCATCCTGAGTCACAGTATTACTTGCTCGAAACATGACACGCCCTGGAAGGTATcgcc                                                                                                              <  2:2620694/150‑1 (MQ=255)
                                    cTTGTTGCTTCGTCGCCAGGGTGGCGGCACTTTTGTCCAGAGCAGCCTATGGCAAAGCTTCAGCGATCCGCTGGTGGAGCTGCTCTCCGACCATCCTGAGTCACAGTATTACTTGCTCGAAACACGACACGCCCTGGAAGGTATcgccgc                                                                                                            <  2:3411496/150‑1 (MQ=255)
                                       gttgCTTCGTCGCCAGGGTGGCGGCACTTTTGTCCAGAGCAGCCTATGGCAAAGCTTCAGCGATCCGCTGGTGGAGCTGCTCTCCGACCATCCTGAGTCACAGTATTACTTGCTCGAAACACGACACGCCCTGGAAGGTATCGCCGCtta                                                                                                         <  2:4150479/150‑1 (MQ=255)
                                        ttgCTTCGTCGCCAGGGTGGCGGCACTTTTGTCCAGAGCAGCCTATGGCAAAGCTTCAGCGATCCGCTGGTGGAGCTGCTCTCCGACCATCCTGAGTCACAGTATTACTTGCTCGAAACACGACACGCCCTGGAAGGTATCGCCGCttat                                                                                                        >  2:3437026/1‑150 (MQ=255)
                                           cTTCGTCGCCAGGGTGGCGGCACTTTTGTCCAGAGCAGCCTATGGCAAAGCTTCAGCGATCCGCTGGTGGAGCTGCTCTCCGACCATCCTGAGTCACAGTATTACTTGCTCGAAACACGACACGCCCTGGAAGGTATCGCCGCTTATTAc                                                                                                     <  1:4274313/150‑1 (MQ=255)
                                            ttCGTCGCCAGGGTGGCGGCACTTTTGTCCAGAGCAGCCTATGGCAAAGCTTCAGCGATCCGCTGGTGGAGCTGCTCTCCGACCATCCTGAGTCACAGTATTACTTGCTCGAAACACGACACGCCCTGGAAGGTATCGCCGCTTATTAcg                                                                                                    <  2:301253/150‑1 (MQ=255)
                                              cgtcgCCAGGGTGGCGGCACTTTTGTCCAGAGCAGCCTATGGCAAAGCTTCAGCGATCCGCTGGTGGAGCTGCTCTCCGACCATCCTGAGTCACAGTATTACTTGCTCGAAACACGACACGCCCTGGAAGGTATCGCCGCTTATTAcgcc                                                                                                  >  2:4055727/1‑150 (MQ=255)
                                               gtcgCCAGGGTGGCGGCACTTTTGTCCAGAGCAGCCTATGGCAAAGCTTCAGCGATCCGCTGGTGGAGCTGCTCTCCGACCATCCTGAGTCACAGTATTACTTGCTCGAAACACGACACGCCCTGGAAGGTATCGCCGCTTATTAcgccg                                                                                                 <  1:4875593/150‑1 (MQ=255)
                                                     aGGGTGGCGGCACTTTTGTCCAGAGCAGCCTATGGCAAAGCTTCAGCGATCCGCTGGTGGAGCTGCTCTCCGACCATCCTGAGTCACAGTATTACTTGCTCGCAACACGACCCGCCCTGGAAGGTCGCGCCGCTGATTACGCCGCGCTGc                                                                                           >  2:3173795/1‑150 (MQ=255)
                                                     aGGGTGGCGGCACTTTTGTCCAGAGCAGCCTATGGCAAAGCTTCAGCGATCCGCTGGTGGAGCTGCTCTCCGACCATCCTGAGTCACAGTATTACTTGCTCGAAACACGACACGCCCTGGAAGGTATCGCCGCTTATTACGCCGCGCTGc                                                                                           >  1:3524816/1‑150 (MQ=255)
                                                     aGGGTGGCGGCACTTTTGTCCAGAGCAGCCTATGGCAAAGCTTCAGCGATCCGCTGGTGGAGCTGCTCTCCGACCATCCTGAGTCACAGTATTACTTGCTCGAAACACGACACGCCCTGGAAGGTATCGCCGCTTATTACGCCGCGCTGc                                                                                           >  2:4859554/1‑150 (MQ=255)
                                                     aGGGTGGCGGCACTTTTGTCCAGAGCAGCCTATGGCAAAGCTTCAGCGATCCGCTGGTGGAGCTGCTCTCCGACCATCCTGAGTCACAGTATTACTTGCTCGAAACACGACACGCCCTGGAAGGTATCGCCGCTTATTACGCCGCGCTGc                                                                                           >  1:891561/1‑150 (MQ=255)
                                                     aGGGTGGCGACACTTTTGTCCAGAGCAGCCTATGGCAAAGCTTCAGCGATCCGCTGGTGGAGCTGCTCTCCGACCATCCTGAGTCACAGTATTACTTGCTCGAAACACGACACGCCCTGGAAGGTATCGCCGCTTATTACGCCGCGCTGc                                                                                           <  2:406161/150‑1 (MQ=255)
                                                        gTGGCGGCACTTTTGTCCAGAGCAGCCTATGGCAAAGCTTCAGCGATCCGCTGGTGGAGCTGCTCTCCGACCATCCTGAGTCACAGTATTACTTGCTCGAAACACGACACGCCCTGGAAGGTATCGCCGCTTATTACGCCGCGCTGCgta                                                                                        <  1:1664124/150‑1 (MQ=255)
                                                        gTGGCGGCACTTTTGTCCAGAGCAGCCTATGGCAAAGCTTCAGCGATCCGCTGGTGGAGCTGCTCTCCGACCATCCTGAGTCACAGTATTACTTGCTCGAAACACGACACGCCCTGGAAGGTATCGCCGCTTATTACGCCGCGCTGCgta                                                                                        <  2:5058078/150‑1 (MQ=255)
                                                         tGGCGGCACTTTTGTCCAGAGCAGCCTATGGCAAAGCTTCAGCGATCCGCTGGTGGAGCTGCTCTCCGACCATCCTGAGTCACAGTATTACTTGCTCGAAACACGACACGCCCTGGAAGGTATCGCCGCTTATTACGCCGCGCTGCgtag                                                                                       <  2:2398761/150‑1 (MQ=255)
                                                         tGGCGGCACTTTTGTCCAGAGCAGCCTATGGCAAAGCTTCAGCGATCCGCTGGTGGAGCTGCTCTCCGACCATCCTGAGTCACAGTATTACTTGCTCGAAACACGACACGCCCTGGAAGGTATCGCCGCTTATTACGCCGCGCTGCgtag                                                                                       <  2:4702257/150‑1 (MQ=255)
                                                                 cTTTTGTCCAGAGCAGCCTATGGCAAAGCTTCAGCGATCCGCTGGTGGAGCTGCTCTCCGACCATCCTGAGTCACAGTATTACTTGCTCGAAACACGACACGCCCTGGAAGGTATCGCCGCTTATTACGCCGCGCTGCGTAGTACCGATg                                                                               >  1:216097/1‑150 (MQ=255)
                                                                   tttGTCCAGAGCAGCCTATGGCAAAGCTTCAGCGATCCGCTGGTGGAGCTGCTCTCCGACCATCCTGAGTCACAGTATTACTTGCTCGAAACACGACACGCCCTGGAAGGTATCGCCGCTTATTACGCCGCGCTGCGTAGTACCGATGaa                                                                             <  1:4784115/150‑1 (MQ=255)
                                                                   tttGTCCAGAGCAGCCTATGGCAAAGCTTCAGCGATCCGCTGGTGGAGCTGCTCTCCGACCATCCTGAGTCACAGTATTACTTGCTCGAAACACGACACGCCCTGGAAGGTATCGCCGCTTATTACGCCGCGCTGCGTAGTACCGATGaa                                                                             <  2:4161621/150‑1 (MQ=255)
                                                                    ttGTCCAGAGCAGCCTATGGCAAAGCTTCAGCGATCCGCTGGTGGAGCTGCTCTCCGACCATCCTGAGTCACAGTATTACTTGCTCGAAACACGACACGCCCTGGAAGGTATCGCCGCTTATTACGCCGCGCTGCGTAGTACCGATGAAg                                                                            <  1:4584335/150‑1 (MQ=255)
                                                                       tCCAGAGCAGCCTATGGCAAAGCTTCAGCGATCCGCTGGTGGAGCTGCTCTCCGACCATCCTGAGTCACAGTATTACTTGCTCGAAACACGACACGCCCTGGAAGGTATCGCCGCTTATTACGCCGCGCTGCGTAGTACCGATGAAGACa                                                                         <  2:4076264/150‑1 (MQ=255)
                                                                          agagAAGCCTAAGGCAAAGCTTCAGCGATCCTCTGGTGGAGCTGCTCTCCGACCATCATGAGTCACAGTATTACTTGCTAGAAACACGACACGCCCTGGAAGGTATCGCCGCTTATTACGCCGCGCTTCGTAGTACAGATGAAGACAAgg                                                                      >  2:1173794/1‑150 (MQ=255)
                                                                           gagCAGCCTATGGCAAAGCTTCAGCGATCCGCTGGTGGAGCTGCTCTCCGACCATCCTGAGTCACAGTATTACTTGCTCGAAACACGACACGCCCTGGAAGGTATCGCCGCTTATTACGCCGCGCTGCGTAGTACCGATGAAGACAAGGa                                                                     <  1:4197585/150‑1 (MQ=255)
                                                                           gagCAGCCTATGGCAAAGCTTCAGCGATCCGCTGGTGGAGCTGCTCTCCGACCATCCTGAGTCACAGTATTACTTGCTCGAAACACGACACGCCCTGGAAGGTATCGCCGCTTATTACGCCGCGCTGCGTAGTACCGATGAAGACAAGGa                                                                     <  2:2151497/150‑1 (MQ=255)
                                                                            agcagcCTATGGCAAAGCTTCAGCGATCCGCTGGTGGAGCTGCTCTCCGACCATCCTGAGTCACAGTATTACTTGCTCGAAACACGACACGCCCTGGAAGGTATCGCCGCTTATTACGCCGCGCTGCGTAGTACCGATGAAGACAAGGaa                                                                    >  1:3112404/1‑150 (MQ=255)
                                                                             gcagcCTATGGCAAAGCTTCAGCGATCCGCTGGTGGAGCTGCTCTCCGACCATCCTGAGTCACAGTATTACTTGCTCGAAACACGACACGCCCTGGAAGGTATCGCCGCTTATTACGCCGCGCTGCGTAGTACCGATGAAGACAAGGAAc                                                                   <  1:3296147/150‑1 (MQ=255)
                                                                                 ccTATGGCAAAGCTTCAGCGATCCGCTGGTGGAGCTGCTCTCCGACCATCCTGAGTCACAGTATTACTTGCTCGAAACACGACACGCCCTGGAAGGTATCGCCGCTTATTACGCCGCGCTGCGTAGTACCGATGAAGACAAGGAACGCAt                                                               <  2:3568349/150‑1 (MQ=255)
                                                                                 ccTATGGCAAAGCTTCAGCGATCCGCTGGTGGAGCTGCTCTCCGACCATCCTGAGTCACAGTATTACTTGCTCGAAACACGACACGCCCTGGAAGGTATCGCCGCTTATTACGCCGCGCTGCGTAGTACCGATGAAGACAAGGAACGCAt                                                               <  1:2118321/150‑1 (MQ=255)
                                                                                  cTATGGCAAAGCTTCAGCGATCCGCTGGTGGAGCTGCTCTCCGACCATCCTGAGTCACAGTATTACTTGCTCGAAACACGACACGCCCTGGAAGGTATCGCCGCTTATTACGCCGCGCTGCGTAGTACCGATGAAGACAAGGAACGCATc                                                              <  2:487122/150‑1 (MQ=255)
                                                                                   tATGGCAAAGCTTCAGCGATCCGCTGGTGGAGCTGCTCTCCGACCATCCTGAGTCACAGTATTACTTGCTCGAAACACGACACGCCCTGGAAGGTATCGCCGCTTATTACGCCGCGCTGCGTAGTACCGATGAAGACAAGGAACGCATcc                                                             >  2:729312/1‑150 (MQ=255)
                                                                                   tATGGCAAAGCTTCAGCGATCCGCTGGTGGAGCTGCTCTCCGACCATCCTGAGTCACAGTATTACTTGCTCGAAACACGACACGCCCTGGAAGGTATCGCCGCTTATTACGCCGCGCTGCGTAGTACCGATGAAGACAAGGAACGCATcc                                                             <  2:2869709/150‑1 (MQ=255)
                                                                                    aTGGCAAAGCTTCAGCGATCCGCTGGTGGAGCTGCTCTCCGACCATCCTGAGTCACAGTATTACTTGCTCGAAACACGACACGCCCTGGAAGGTATCGCCGCTTATTACGCCGCGCTGCGTAGTACCGATGAAGACAAGGAACGCATCCg                                                            >  1:3592573/1‑150 (MQ=255)
                                                                                     tGGCAAAGCTTCAGCGATCCGCTGGTGGAGCTGCTCTCCGACCATCCTGAGTCACAGTATTACTTGCTCGAAACACGACACGCCCTGGAAGGTATCGCCGCTTATTACGCCGCGCTGCGTAGTACCGATGAAGACAAGGAACGCATCCGt                                                           >  1:515113/1‑150 (MQ=255)
                                                                                         aaaGCTTCAGCGATCCGCTGGTGGAGCTGCTCTCCGACCATCCTGAGTCACAGTATTACTTGCTCGAAACACGACACGCCCTGGAAGGTATCGCCGCTTATTACGCCGCGCTGCGTAGTACCGATGAAGACAAGGAACGCATCCGTGAAc                                                       >  2:4480535/1‑150 (MQ=255)
                                                                                         aaaGCTTCAGCGATCCGCTGGTGGAGCTGCTCTCCGACCATCCTGAGTCACAGTATTACTTGCTCGAAACACGACACGCCCTGGAAGGTATCGCCGCTTATTACGCCGCGCTGCGTAGTACCGATGAAGACAAGGAACGCATCCGTGAAc                                                       <  2:3027156/150‑1 (MQ=255)
                                                                                         aaaGCTTCAGCGATCCGCTGGTGGAGCTGCTCTCCGACCATCCTGAGTCACAGTATTACTTGCTCGAAACACGACACGCCCTGGAAGGTATCGCCGCTTATTACGCCGCGCTGCGTAGTACCGATGAAGACAAGGAACGCATCCGTGAAc                                                       >  2:2163335/1‑150 (MQ=255)
                                                                                         aaaGCTTCAGCGATCCGCTGGTGGAGCTGCTCTCCGACCATCCTGAGTCACAGTATTACTTGCTCGAAACACGACACGCCCTGGAAGGTATCGCCGCTTATTACGCCGCGCTGCGTAGTACCGATGAAGACAAGGAACGCATCCGTGAAc                                                       <  2:2107980/150‑1 (MQ=255)
                                                                                          aaGCTTCAGCGATCCGCTGGTGGAGCTGCTCTCCGACCATCCTGAGTCACAGTATTACTTGCTCGAAACACGACACGCCCTGGAAGGTATCGCCGCTTATTACGCCGCGCTGCGTAGTACCGATGAAGACAAGGAACGCATCCGTGAACt                                                      >  1:1238363/1‑150 (MQ=255)
                                                                                            gCTTCAGCGATCCGCTGGTGGAGCTGCTCTCCGACCATCCTGAGTCACAGTATTACTTGCTCGAAACACGACACGCCCTGGAAGGTATCGCCGCTTATTACGCCGCGCTGCGTAGTACCGATGAAGACAAGGAACGCATCCGTGAACTcc                                                    <  2:1693753/150‑1 (MQ=255)
                                                                                            gCTTCAGCGATCCGCTGGTGGAGCTGCTCTCCGACCATCCTGAGTCACAGTATTACTTGCTCGAAACACGACACGCCCTGGAAGGTATCGCCGCTTATTACGCCGCGCTGCGTAGTACCGATGAAGACAAGGAACGCATCCGTGAACTcc                                                    <  2:512035/150‑1 (MQ=255)
                                                                                              ttCAGCGATCCGCTGGTGGAGCTGCTCTCCGACCATCCTGAGTCACAGTATTACTTGCTCGAAACACGACACGCCCTGGAAGGTATCGCCGCTTATTACGCCGCGCTGCGTAGTACCGATGAAGACAAGGAACGCATCCGTGAACTccac                                                  <  2:3540854/150‑1 (MQ=255)
                                                                                                cAGCGATCCGCTGGTGGAGCTGCTCTCCGACCATCCTGAGTCACAGTATTACTTGCTCGAAACACGACACGCCCTGGAAGGTATCGCCGCTTATTACGCCGCGCTGCGTAGTACCGATGAAGACAAGGAACGCATCCGTGAACTccacca                                                <  2:5092894/150‑1 (MQ=255)
                                                                                                 aGCGATCCGCTGGTGGAGCTGCTCTCCGACCATCCTGAGTCACAGTATTACTTGCTAGAAACACGACACGCCCTGGAAGGTATCGCCGCTTATTACGCCCCGCAGCGTAGAAACCATGAAGACAAGGAACGCATCCGTGAACTCcaccac                                               >  1:2643963/1‑150 (MQ=255)
                                                                                                    gATCCGCTGGTGGAGCTGCTCTCCGACCATCCTGAGTCACAGTATTACTTGCTCGAAACACGACACGCCCTGGAAGGTATCGCCGCTTATTACGCCGCGCTGCGTAGTACCGATGAAGACAAGGAACGCATCCGTGAACTCCACCACGcc                                            >  1:684580/1‑150 (MQ=255)
                                                                                                     aTCCGCTGGTGGAGCTGCTCTCCGACCATCCTGAGTCACAGTATTACTTGCTCGAAACACGACACGCCCTGGAAGGTATCGCCGCTTATTACGCCGCGCTGCGTAGTACCGATGAAGACAAGGAACGCATCCGTGAACTCCACCACGCCa                                           >  1:1076032/1‑150 (MQ=255)
                                                                                                        cGCTGGTGGAGCTGCTCTCCGACCATCCTGAGTCACAGTATTACTTGCTCGAAACACGACACGCCCTGGAAGGTATCGCCGCTTATTACGCCGCGCTGCGTAGTACCGATGAAGACAAGGAACGCATCCGTGAACTCCACCACGCCATag                                        >  2:613818/1‑150 (MQ=255)
                                                                                                         gCTGGTGGAGCTGCTCTCCGACCATCCTGAGTCACAGTATTACTTGCTCGAAACACGACACGCCCTGGAAGGTATCGCCGCTTATTACGCCGCGCTGCGTAGTACCGATGAAGACAAGGAACGCATCCGTGAACTCCACCACGCCATaga                                       >  2:422906/1‑150 (MQ=255)
                                                                                                           tggtggAGCTGCTCTCCGACCATCCTGAGTCACAGTATTACTTGCTCGAAACACGACACGCCCTGGAAGGTATCGCCGCTTATTACGCCGCGCTGCGTAGTACCGATGAAGACCAGGAACGCATCCGTGAACTCAACCACGCCCTAGAGc                                     >  1:2859854/1‑150 (MQ=255)
                                                                                                             gtggAGCTGCTCTCCGACCATCCTGAGTCACAGTATTACTTGCTCGAAACACGACACGCCCTGGAAGGTATCGCCGCTTATTACGCCGCGCTGCGTAGTACCGATGAAGACAAGGAACGCATCCGTGAACTCCACCACGCCATAGAGCTg                                   >  1:239256/1‑150 (MQ=255)
                                                                                                             gtggAGCTGCTCTCCGACCATCCTGAGTCACAGTATGACTTGCTCGAAACACGACACGCCCTGGAAGGTATCGCCGCTTATTACGCCGCGCTGCGTAGTACCGATGAAGACAAGGAACGCATCCGTGAACTCCACCACGCCATAGAGCTg                                   <  2:1726956/150‑1 (MQ=255)
                                                                                                               ggAGCTGCTCTCCGACCATCCTGAGTCACAGTATTACTTGCTCGAAACACGACACGCCCTGGAAGGTATCGCCGCTTATTACGCCGCGCTGCGTAGTACCGATGAAGACAAGGAACGCATCCGTGAACTCCACCACGCCATAGAGCTGgc                                 <  2:4994833/150‑1 (MQ=255)
                                                                                                               ggAGCTGCTCTCCGACCATCCTGAGTCACAGTATTACTTGCTCGAAACACGACACGCCCTGGAAGGTATCGCCGCTTATTACGCCGCGCTGCGTAGTACCGATGAAGACAAGGAACGCATCCGTGAACTCCACCACGCCATAGAGCTGgc                                 <  1:3927199/150‑1 (MQ=255)
                                                                                                               ggAGCTGCTCTCCGACCATCCTGAGTCACAGTATTACTTGCTCGAAACACGACACGCCCTGGAAGGTATCGCCGCTTATTACGCCGCGCTGCGTAGTACCGATGAAGACAAGGAACGCATCCGTGAACTCCACCACGCCATAGAGCTGgc                                 <  2:3402171/150‑1 (MQ=255)
                                                                                                                    tgctCTCCGACCATCCTGAGTCACAGTATTACTTGCTCGAAACACGACACGCCCTGGAAGGTATCGCCGCTTATTACGCCGCGCTGCGTAGTACCGATGAAGACAAGGAACGCATCCGTGAACTCCACCACGCCATAGAGCTGGCgcagc                            <  2:4277227/150‑1 (MQ=255)
                                                                                                                    tgctCTCCGACCATCCTGAGTCACAGTATTACTTGCTCGAAACACGACACGCCCTGGAAGGTATCGCCGCTTATTACGCCGCGCTGCGTAGTACCGATGAAGACAAGGAACGCATCCGTGAACTCCACCACGCCATAGAGCTGGCgcagc                            >  2:542534/1‑150 (MQ=255)
                                                                                                                    tgctCTCCGACCATCCTGAGTCACAGTATTACTTGCTCGAAACACGACACGCCCTGGAAGGTATCGCCGCTTATTACGCCGCGCTGCGTAGTACCGATGAAGACAAGGAACGCATCCGTGAACTCCACCACGCCATAGAGCTGGCgcagc                            >  2:2568386/1‑150 (MQ=255)
                                                                                                                      ctctCCGACCATCCTGAGTCACAGTATTACTTGCTCGAAACACGACACGCCCTGGAAGGTATCGCCGCTTATTACGCCGCGCTGCGTAGTACCGATGAAGACAAGGAACGCATCCGTGAACTCCACCACGCCATAGAGCTGGCGccgcag                          <  2:4772825/150‑1 (MQ=255)
                                                                                                                      ctctCCGACCATCCTGAGTCACAGTATTACTTGCTCGAAACACGACACGCCCTGGAAGGTATCGCCGCTTATTACGCCGCGCTGCGTAGTACCGATGAAGACAAGGAACGCATCCGTGAACTCCACCACGCCATAGAGCTGGCGcagcag                          >  2:4452302/1‑150 (MQ=255)
                                                                                                                        ctcCGACCATCCTGAGTCACAGTATTACTTGCTCGAAACACGACACGCCCTGGAAGGTATCGCCGCTTATTACGCCGCGCTGCGTAGTACCGATGAAGACAAGGAACGCATCCGTGAACTCCACCACGCCATAGAGCTGGCGCAGCAGTc                        <  1:3998284/150‑1 (MQ=255)
                                                                                                                         tcCGACCATCCTGAGTCACAGTATTACTTGCTCGAAACACGACACGCCCTGGAAGGTATCGCCGCTTATTACGCCGCGCTGCGTAGTACCGATGAAGACAAGGAACGCATCCGTGAACTCCACCACGCCATAGAGCTGGCGCAGCAGTCt                       <  2:1862425/150‑1 (MQ=255)
                                                                                                                         tcCGACCATCCTGAGTCACAGTATTACTTGCTCGAAACACGACACGCCCTGGAAGGTATCGCCGCTTATTACGCCGCGCTGCGTAGTACCGATGAAGACAAGGAACGCATCCGTGAACTCCACCACGCCATAGAGCTGGCGCAGCAGTCt                       >  1:3740363/1‑150 (MQ=255)
                                                                                                                          ccGACCATCCTGAGTCACAGTATTACTTGCTCGAAACACGACACGCCCTGGAAGGTATCGCCGCTTATTACGCCGCGCTGCGTAGTACCGATGAAGACAAGGAACGCATCCGTGAACTCCACCACGCCATAGAGCTGGCGCAGCAGTCTg                      >  1:4120914/1‑150 (MQ=255)
                                                                                                                              ccATCCTGAGTCACAGTATTACTTGCTCGAAACACGACACGCCCTGGAAGGTATCGCCGCTTATTACGCCGCGCTGCGTAGTACCGATGAAGACAAGGAACGCATCCGTGAACTCCACCACGCCATAGAGCTGGCGCAGCAGTCTGGCGa                  <  1:3641257/150‑1 (MQ=255)
                                                                                                                                     gAGTCACAGTATTACTTGCTCGAAACACGACACGCCCTGGAAGGTATCGCCGCTTATTACGCCGCGCTGCGTAGTACCGATGAAGACAAGGAACGCATCCGTGAACTCCACCACGCCATAGAGCTGGCGCAGCAGTCTGGCGATCTGGAc           <  1:1534436/150‑1 (MQ=255)
                                                                                                                                     gAGTCACAGTATTACTTGCTCGAAACACGACACGCCCTGGAAGGTATCGCCGCTTATTACGCCGCGCTGCGTAGTACCGATGAAGACAAGGAACGCATCCGTGAACTCCACCACGCCATAGAGCTGGCGCAGCAGTCTGGCGATCTGGAc           <  2:4744546/150‑1 (MQ=255)
                                                                                                                                     gAGTCACAGTATTACGTGCTCGAAACACGACACTCCCTGGAAGGTATCGCCGCTTATTACGCCGCGCTGCGTAGTACCGATGAAGACAAGGAACGCATCCGTGAACTCCACCACGCCATAGAGCTGGCGCAGCAGTCTGGCGATCTGGAc           <  2:4744654/150‑1 (MQ=255)
                                                                                                                                          acaGTATTACTTGCTCGAAACACGACACGCCCTGGAAGGTATCGCCGCTTATTACGCCGCGCTGCGTAGTACCGATGAAGACAAGGAACGCATCCGTGAACTCCACCACGCCATAGAGCTGGCGCAGCAGTCTGGCGATCTGGACGCGGa      >  2:2305552/1‑150 (MQ=255)
                                                                                                                                            aGTATTACTTGCTCGAAACACGACACGCCCTGGAAGGTATCGCCGCTTATTACGCCGCGCTGCGTAGTACCGATGAAGACAAGGAACGCATCCGTGAACTCCACCACGCCATAGAGCTGGCGCAGCAGTCTGGCGATCTGGACGCGGAAt    >  2:3369769/1‑150 (MQ=255)
                                                                                                                                            aGTATTACTTGCTCGAAACACGACACGCCCTGGAAGGTATCGCCGCTTATTACGCCGCGCTGCGTAGTACCGATGAAGACAAGGAACGCATCCGTGAACTCCACCACGCCATAGAGCTGGCGCAGCAGTCTGGCGATCTGGACGCGGAAt    >  2:3826981/1‑150 (MQ=255)
                                                                                                                                             gTATTACTTGCTCGAAACACGACACGCCCTGGAAGGTATCGCCGCTTATTACGCCGCGCTGCGTAGTACCGATGAAGACAAGGAACGCATCCGTGAACTCCACCACGCCATAGAGCTGGCGCAGCAGTCTGGCGATCTGGACGCGGACTc   >  2:4662590/1‑150 (MQ=255)
                                                                                                                                             gTATTACTTGCTCGAAACACGACACGCCCTGGAAGGTATCGCCGCTTATTACGCCGCGCTGCGTAGTACCGATGAAGACAAGGAACGCATCCGTGAACTCCACCACGCCATAGAGCTGGCGCAGCAGTCTGGCGATCTGGACGCGGAATc   >  2:3450508/1‑150 (MQ=255)
                                                                                                                                             gTATTACTTGCTCGAAACACGACACGCCCTGGAAGGTATCGCCGCTTATTACGCCGCGCTGCGTAGTACCGATGAAGACAAGGAACGCATCCGTGAACTCCACCACGCCATAGAGCTGGCGCAGCAGTCTGGCGATCTGGACGCGGAATc   >  2:64951/1‑150 (MQ=255)
                                                                                                                                              tATTACTTGCTCGAAACACGACACGCCCTGGAAGGTATCGCCGCTTATTACGCCGCGCTGCGTAGTACCGATGAAGACAAGGAACGCATCCGTGAACTCCACCACGCCATAGAGCTGGCGCAGCAGTCTGGCGATCTGGACGCGGAATCa  <  2:764750/150‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                  
CTTGCGTGAGGCGATTCAACGTCTCGAAGCGAAGGGCTTGTTGCTTCGTCGCCAGGGTGGCGGCACTTTTGTCCAGAGCAGCCTATGGCAAAGCTTCAGCGATCCGCTGGTGGAGCTGCTCTCCGACCATCCTGAGTCACAGTATGACTTGCTCGAAACACGACACGCCCTGGAAGGTATCGCCGCTTATTACGCCGCGCTGCGTAGTACCGATGAAGACAAGGAACGCATCCGTGAACTCCACCACGCCATAGAGCTGGCGCAGCAGTCTGGCGATCTGGACGCGGAATCA  >  CP009273/118728‑119019

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑