BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	2,074,680	A→T	36.4%	W22R (TGG→AGG)	sbmC ←	DNA gyrase inhibitor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	2,074,680	0	A	T	36.4%	268.8 / 297.9	244	W22R (TGG→AGG)	sbmC	DNA gyrase inhibitor
Reads supporting (aligned to +/- strand): ref base A (74/81); new base T (39/50); total (113/131)
Fisher's exact test for biased strand distribution p-value = 5.95e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.65e-01

Only 100 of 254 total aligned reads displayed.

CACCGTCACGACGGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCACGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGCAAAATTAAAACCACAAGGAATGAGTGTTGATTATGCGAGCAGACT  >  CP009273/2074531‑2074829

cACCGTCACGACGGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCa                                                                                                                                                       >  1:5155972/1‑150 (MQ=255)

cACCGTCACGACGGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCa                                                                                                                                                       >  1:4314336/1‑150 (MQ=255)

 aCCGTCACGACGGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCAc                                                                                                                                                      <  1:2334517/150‑1 (MQ=255)

    gTCACGACGGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCggg                                                                                                                                                   >  2:486206/1‑150 (MQ=255)

     tCACGACGGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCACGGGc                                                                                                                                                  >  2:1675693/1‑150 (MQ=255)

     tCACGACGGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCACGGGc                                                                                                                                                  >  1:30270/1‑150 (MQ=255)

      cACGACGGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGcc                                                                                                                                                 <  2:1095480/150‑1 (MQ=255)

         gacgGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCACGGGCCAAc                                                                                                                                              <  2:2340578/150‑1 (MQ=255)

          acgGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACg                                                                                                                                             <  1:404243/150‑1 (MQ=255)

          acgGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACg                                                                                                                                             <  1:2758878/150‑1 (MQ=255)

              tgtCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCACGGGCCAACGAGAt                                                                                                                                         >  1:3441593/1‑150 (MQ=255)

               gtCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATg                                                                                                                                        >  2:3883552/1‑150 (MQ=255)

                   cAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGaaa                                                                                                                                    >  1:3994994/1‑150 (MQ=255)

                     gCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCACGGGCCAACGAGATGGAAAcc                                                                                                                                  <  2:2792479/150‑1 (MQ=255)

                      cGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCt                                                                                                                                 <  2:1282402/150‑1 (MQ=255)

                        tAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGc                                                                                                                               <  1:3276810/150‑1 (MQ=255)

                        tAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGc                                                                                                                               >  1:1972263/1‑150 (MQ=255)

                         aaTTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCa                                                                                                                              >  1:1552328/1‑150 (MQ=255)

                          aTTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCACGGGCCAACGAGATGGAAACCTGCaa                                                                                                                             >  1:1400172/1‑150 (MQ=255)

                          aTTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCACGGGCCAACGAGATGGAAACCTGCaa                                                                                                                             >  1:1653292/1‑150 (MQ=255)

                              ttCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCACGGGCCAACGAGATGGAAACCTGCAACGGt                                                                                                                         <  2:4493285/150‑1 (MQ=255)

                                cggcggGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCACGGGCCAACGAGATGGAAACCTGCAACGGTAc                                                                                                                       >  1:3507846/1‑150 (MQ=255)

                                  gcggGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCACGGGCCAACGAGATGGAAACCTGCAACGGTACGt                                                                                                                     <  1:2141061/150‑1 (MQ=255)

                                   cggGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGtt                                                                                                                    <  1:3050233/150‑1 (MQ=255)

                                   cggGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCACGGGCCAACGAGATGGAAACCTGCAACGGTACGtt                                                                                                                    >  2:1405303/1‑150 (MQ=255)

                                   cggGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCACGGGCCAACGAGATGGAAACCTGCAACGGTACGtt                                                                                                                    >  2:2860185/1‑150 (MQ=255)

                                     ggTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCATCTTCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTtc                                                                                                                  <  1:694666/150‑1 (MQ=255)

                                        gtgtCATCTGGATTGTCGTAATAGACAGCAACCCACTCCGTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCACGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTctct                                                                                                               <  2:3436839/147‑1 (MQ=255)

                                        gtTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCACGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTctct                                                                                                               >  2:2795017/1‑150 (MQ=255)

                                               cTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCACGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCt                                                                                                        >  1:4158943/1‑150 (MQ=255)

                                                  gATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTaa                                                                                                     <  2:720200/150‑1 (MQ=255)

                                                  gATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTaa                                                                                                     <  2:58772/150‑1 (MQ=255)

                                                     tGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAAtct                                                                                                  <  2:1943667/150‑1 (MQ=255)

                                                     tGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAAtct                                                                                                  <  2:5174226/150‑1 (MQ=255)

                                                      gTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCACGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAAtctc                                                                                                 >  1:5003348/1‑150 (MQ=255)

                                                      gTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCACGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAAtctc                                                                                                 <  2:2142453/150‑1 (MQ=255)

                                                        cGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCACGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGt                                                                                               <  1:855396/150‑1 (MQ=255)

                                                           aaTAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGt                                                                                            >  2:3934210/1‑150 (MQ=255)

                                                             tAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTc                                                                                          <  1:50476/150‑1 (MQ=255)

                                                             tAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTc                                                                                          <  1:50445/150‑1 (MQ=255)

                                                             tAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCACGGGCCAACGAGATGGACACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTc                                                                                          >  2:3312469/1‑150 (MQ=255)

                                                              aGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCa                                                                                         <  1:514821/150‑1 (MQ=255)

                                                                aCAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCACGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATg                                                                                       <  1:1278952/150‑1 (MQ=255)

                                                                  aGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGAc                                                                                     >  2:3714920/1‑150 (MQ=255)

                                                                    cAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGc                                                                                   >  2:266163/1‑150 (MQ=255)

                                                                        ccACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCACGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTcc                                                                               >  2:1107472/1‑150 (MQ=255)

                                                                         cACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCACGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCa                                                                              <  1:654693/150‑1 (MQ=255)

                                                                         cACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCACGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCa                                                                              >  2:1292918/1‑150 (MQ=255)

                                                                          aCTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCACGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCAt                                                                             >  2:5098198/1‑150 (MQ=255)

                                                                           cTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTGACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCAtt                                                                            <  1:3725497/150‑1 (MQ=255)

                                                                              cTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCACGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGAt                                                                         <  2:5082690/150‑1 (MQ=255)

                                                                                tCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAACCCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATAc                                                                       >  1:231618/1‑150 (MQ=255)

                                                                                tCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATAc                                                                       >  1:4912735/1‑150 (MQ=255)

                                                                                tCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATAc                                                                       <  1:3300305/150‑1 (MQ=255)

                                                                                 cGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACt                                                                      >  1:1945264/1‑150 (MQ=255)

                                                                                  ggCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTg                                                                     >  1:4593900/1‑150 (MQ=255)

                                                                                  ggCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTg                                                                     >  1:2911725/1‑150 (MQ=255)

                                                                                  ggCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCACGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTg                                                                     >  2:2685966/1‑150 (MQ=255)

                                                                                  ggCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCACGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTg                                                                     >  1:3578257/1‑150 (MQ=255)

                                                                                       aaTATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCACGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGttttt                                                                <  1:150821/150‑1 (MQ=255)

                                                                                         tatTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCACGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTtat                                                              >  2:3604347/1‑150 (MQ=255)

                                                                                            ttttGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTtatata                                                           <  1:2473500/150‑1 (MQ=255)

                                                                                              ttGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCACGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACa                                                         <  1:1829124/150‑1 (MQ=255)

                                                                                                gCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCACGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGt                                                       <  1:3835497/150‑1 (MQ=255)

                                                                                                 cTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCACGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGta                                                      >  1:1040336/1‑150 (MQ=255)

                                                                                                 cTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCACGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGta                                                      >  1:4691324/1‑150 (MQ=255)

                                                                                                 cTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCACGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGta                                                      >  1:4754921/1‑150 (MQ=255)

                                                                                                    tCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAg                                                   <  2:1549914/150‑1 (MQ=255)

                                                                                                      tACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCACGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGtt                                                 >  1:5167000/1‑150 (MQ=255)

                                                                                                      tACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCACGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGtt                                                 >  1:2035948/1‑150 (MQ=255)

                                                                                                      tACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCACGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGtt                                                 <  2:4740520/150‑1 (MQ=255)

                                                                                                      tACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCACGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATAAAGTATAGtt                                                 >  2:905310/1‑150 (MQ=255)

                                                                                                       aCCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTg                                                <  1:4485249/150‑1 (MQ=255)

                                                                                                        cccACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGc                                               >  2:3156046/1‑150 (MQ=255)

                                                                                                         ccACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCACGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGCa                                              <  2:1896570/150‑1 (MQ=255)

                                                                                                         ccACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCACGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGCa                                              <  2:1896425/150‑1 (MQ=255)

                                                                                                          cacaTCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGCaa                                             >  1:2850146/1‑150 (MQ=255)

                                                                                                           acaTCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGCaaa                                            >  1:2127274/1‑150 (MQ=255)

                                                                                                             atcatcAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGCAAAAt                                          >  2:4355493/1‑150 (MQ=255)

                                                                                                              tcatcaACTGCTCAATTCCTTTCTTTACCTTCTGTTCCCACGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGGTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGCAAAAtt                                         <  1:5104148/150‑1 (MQ=255)

                                                                                                                  caACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATATGTGCAAAATTaaaa                                     >  2:5015941/1‑150 (MQ=255)

                                                                                                                    aCTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCACGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGCAAAATTAAAAcc                                   >  1:3378828/1‑150 (MQ=255)

                                                                                                                    aCTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCACGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGCAAAATTAAAAcc                                   <  1:3204224/150‑1 (MQ=255)

                                                                                                                     cTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCACGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGCAAAATTAAAACca                                  <  1:1722422/150‑1 (MQ=255)

                                                                                                                      tGCTCAAAGCCTTTCTTTACCGTCTGTTCCCACGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGCAAAATTAAAACcac                                 >  2:1015993/1‑150 (MQ=255)

                                                                                                                       gCTCAAAGCCTTTCTTTACCGTCTGTTCCCACGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGCAAAATTAAAACcaca                                >  1:5098847/1‑150 (MQ=255)

                                                                                                                        cTCAAAGCCTTTCTTTACCGTCTGTTCCCACGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGCAAAATTAAAACCACaa                               >  2:4618285/1‑150 (MQ=255)

                                                                                                                         tCAAAGCCTTTCTTTACCGTCTGTTCCCACGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGCAAAATTAAAACCACAAg                              <  2:2434648/150‑1 (MQ=255)

                                                                                                                            aaGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGCAAAATTAAAACCACAAGGaa                           >  2:4589406/1‑150 (MQ=255)

                                                                                                                                ctttctttACCGTCTGTTCCCACGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTCCAAAATTAAAACCACAAGGAATGAg                       >  2:2882381/1‑150 (MQ=255)

                                                                                                                                 tttctttACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGCAAAATTAAAACCACAAGGAATGAgt                      >  1:2071989/1‑150 (MQ=255)

                                                                                                                                 tttctttACCGTCTGTTCCCACGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGCAAAATTAAAACCACAAGGAATGAgt                      <  2:3994457/150‑1 (MQ=255)

                                                                                                                                 atactttACCCTCTGTACCCACGGGCCAACGAGAAGGAAACCTGCAAAGCTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGAATAGATGCAAAATTAAAACCACAAGGAATGAga                      >  1:1122333/4‑149 (MQ=255)

                                                                                                                                  ttctttACCGTCTGTTCCCACGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGCAAAATTAAAACCACAAGGAATGAgtg                     <  2:5135476/150‑1 (MQ=255)

                                                                                                                                     tttACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGCAAAATTAAAACCACAAGGAATGAGTGTTg                  <  2:3104301/150‑1 (MQ=255)

                                                                                                                                     tttACCGTCTGGTCCCTCTGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGCAAAATTAAAACCACAAGGAATGAGTGTTg                  <  2:498899/150‑1 (MQ=255)

                                                                                                                                      ttACCGTCTGTTCCCTCGGTCAAACGAGATGGAAACCTGCAACGGTATGTTTCTCTTCCTGCTTAATCTCGTAGTTCTTGCCGACTCCATTGATACTGTTTTTTTATACAGTATTGTTGCAAATTTAAAACCACAAGGAATGAGTGTTGa                 <  1:484072/150‑1 (MQ=255)

                                                                                                                                      ttACCGTCTGTTCCCACGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGCAAAATTAAAACCACAAGGAATGAGTGTTGa                 <  1:4215658/150‑1 (MQ=255)

                                                                                                                                            tCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGCAAAATTAAAACCACAAGGAATGAGTGTTGATTATGc           >  2:77709/1‑150 (MQ=255)

                                                                                                                                                     aCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGCAAAATTAAAACCACAAGGAATGAGTGTTGATTATGCGAGCAGACt  >  1:4669693/1‑150 (MQ=255)

CACCGTCACGACGGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCACGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGCAAAATTAAAACCACAAGGAATGAGTGTTGATTATGCGAGCAGACT  >  CP009273/2074531‑2074829

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`