BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	2,136,421	A→G	12.1%	D3D (GAT→GAC)	udk ←	uridine/cytidine kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	2,136,421	0	A	G	12.1%	674.2 / 65.5	239	D3D (GAT→GAC)	udk	uridine/cytidine kinase
Reads supporting (aligned to +/- strand): ref base A (117/93); new base G (14/15); total (131/108)
Fisher's exact test for biased strand distribution p-value = 5.51e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 243 total aligned reads displayed.

TGATCTTTGTAATAGCAGTCTTCGGGAATTACGCCGATGTGTTCATCACCGACTTGCTCACGCAATTCACGATAAAGGGTACTGGCAATAAGACTCTTGCCGGAAGCCGATGCGCCAGCGATACCGATAATGACGCACTGATGAGACTGATCAGTCATATATTTAGCGACCTGATTAACCTGGATGTTAGGAAGGGGGCGACGAAGCGCCAAACGCGGCAATTATAGGGATTTCATCCGTCTGATACCAGTCGAATAGCGTTGCCGCGCGCTCAGAGTTAATTGTTGACAAAGAATTCC  >  CP009273/2136272‑2136570

       tGTAATAGCAGTCTTCGGGAATTACGCCGATGTGTTCATCACCGACTTGCTCACGCAATTCACGATAAAGGGTACTGGCAATAAGACTCTTGCCGGAAGCCGATGCGCCAGCGATACCGATAATGACGCACTGATGAGACTGATCAGTCa                                                                                                                                                >  2:4219182/1‑150 (MQ=255)

        gTAATAGCAGTCTTCGGGAATTACGCCGATGTGTTCATCACCGACTTGCTCACGCAATTCACGATAAAGGGTACTGGCAATAAGACTCTTGCCGGAAGCCGATGCGCCAGCGATACCGATAATGACGCACTGATGAGACTGATCAGTCat                                                                                                                                               >  2:923583/1‑150 (MQ=255)

        gTAATAGCAGTCTTCGGGAATTACGCCGATGTGTTCATCACCGACTTGCTCACGCAATTCACGATAAAGGGTACTGGCAATAAGACTCTTGCCGGAAGCCGATGCGCCAGCGATACCGATAATGACGCACTGATGAGACTGATCAGTCat                                                                                                                                               >  2:1311357/1‑150 (MQ=255)

        gTAATAGCAGTCTTCGGGAATTACGCCGATGTGTTCATCACCGACTTGCTCACGCAATTCACGATAAAGGGTACTGGCAATAAGACTCTTGCCGGAAGCCGATGCGCCAGCGATACCGATAATGACGCACTGATGAGACTGATCAGTCat                                                                                                                                               <  2:3327949/150‑1 (MQ=255)

           aTAGCAGTCTTCGGGAATTACGCCGATGTGTTCATCACCGACTTGCTCACGCAATTCACGATAAAGGGTACTGGCAATAAGACTCTTGCCGGAAGCCGATGCGCCAGCGATACCGATAATGACGCACTGATGAGACTGATCAGTCatata                                                                                                                                            >  1:3644469/1‑150 (MQ=255)

              gCAGTCTTCGGGAATTACGCCGATGTGTTCATCACCGACTTGCTCACGCAATTCACGATAAAGGGTACTGGCAATAAGACTCTTGCCGGAAGCCGATGCGCCAGCGATACCGATAATGACGCACTGATGAGACTGATCAGTCATATAttt                                                                                                                                         <  2:1469326/150‑1 (MQ=255)

              gCAGTCTTCGGGAATTACGCCGATGTGTTCATCACCGACTTGCTCACGCAATTCACGATAAAGGGTACTGGCAATAAGACTCTTGCCGGAAGCCGATGCGCCAGCGATACCGATAATGACGCACTGATGAGACTGATCAGTCATATAttt                                                                                                                                         >  2:3492108/1‑150 (MQ=255)

               cAGTCTTCGGGAATTACGCCGATGTGTTCATCACCGACTTGCTCACGCAATTCACGATAAAGGGTACTGGCAATAAGACTCTTGCCGGAAGCCGATGCGCCAGCGATACCGATAATGACGCACTGATGAGACTGATCAGTCATATATTTa                                                                                                                                        >  1:3226871/1‑150 (MQ=255)

                 gTCTTCGGGAATTACGCCGATGTGTTCATCACCGACTTGCTCACGCAATTCACGATAAAGGGTACTGGCAATAAGACTCTTGCCGGAAGCCGATGCGCCAGCGATACCGATAATGACGCACTGATGAGACTGATCAGTCATATATTTAGc                                                                                                                                      >  2:5092805/1‑150 (MQ=255)

                    ttCGGGAATTACGCCGATGTGTTCATCACCGACTTGCTCACGCAATTCACGATAAAGGGTACTGGCAATAAGACTCTTGCCGGAAGCCGATGCGCCAGCGATACCGATAATGACGCACTGATGAGACTGATCAGTCATATATTTAGCGAc                                                                                                                                   <  1:1518741/150‑1 (MQ=255)

                     tCGGGAATTACGCGGATGTGTTCATCACCGACTTGCTCACGCAATTCACGATAAAGGGTACTGGCAATAAGACTCTTGCCGGAAGCCGATGCGCCAGCGATACCGATAATGACTCACTGATGAGACTGATCAGTCATATATTTAGCGAcc                                                                                                                                  <  2:4436572/150‑1 (MQ=255)

                      ngggAATTACGCCGATGTGTTCATCACCGACTTGCTCACGCAATTCACGATAAAGGGTACTGGCAATAAGACTCTTGCCGGAAGCCGATGCGCCAGCGATACCGATAATGACGCACTGATGAGACTGATCAGTCATATATTTAGCGACCt                                                                                                                                 >  1:3014133/2‑150 (MQ=255)

                      cGGGAATTACGCCGATGTGTTCATCACCGACTTGCTCACGCAATTCACGATAAAGGGTACTGGCAATAAGACTCTTGCCGGAAGCCGATGCGCCAGCGATACCGATAATGACGCACTGATGAGACTGGTCAGTCATATATTTAGCGACCt                                                                                                                                 >  2:1538394/1‑150 (MQ=255)

                       gggAATTACGCCGATGTGTTCATCACCGACTTGCTCACGCAATTCACGATAAAGGGTACTGGCAATAAGACTCTTGCCGGAAGCCGATGCGCCAGCGATACCGATAATGACGCACTGATGAGACTGGTCAGTCATATATTTAGCGACCTg                                                                                                                                >  1:2957296/1‑150 (MQ=255)

                        ggAATTACGCCGATGTGTTCATCACCGACTTGCTCACGCAATTCACGATAAAGGGTACTGGCAATAAGACTCTTGCCGGAAGCCGATGCGCCAGCGATACCGATAATGACGCACTGATGAGACTGATCAGTCATATATTTAGCGACCTGa                                                                                                                               <  1:2942454/150‑1 (MQ=255)

                         gAATTACGCCGATGTGTTCATCACCGACTTGCTCACGCAATTCACGATAAAGGGTACTGGCAATAAGACTCTTGCCGGAAGCCGATGCGCCAGCGATACCGATAATGACGCACTGATGAGACTGATCAGTCATATATTTAGCGACCTGAt                                                                                                                              >  2:4672949/1‑150 (MQ=255)

                          aaTTACGCCGATGTGTTCATCACCGACTTGCTCACGCAATTCACGATAAAGGGTACTGGCAATAAGACTCTTGCCGGAAGCCGATGCGCCAGCGATACCGATAATGACGCACTGATGAGACTGATCAGTCATATATTTAGCGACCTGAtt                                                                                                                             <  1:492835/150‑1 (MQ=255)

                             tACGCCGATGTGTTCATCACCGACTTGCTCACGCAATTCACGATAAAGGGTACTGGCAATAAGACTCTTGCCGGAAGCCGATGCGCCAGCGATACCGATAATGACGCACTGATGAGACTGGTCAGTCATATATTTAGCGACCTGATTAAc                                                                                                                          >  2:1024429/1‑150 (MQ=255)

                             tACGCCGATGTGTTCATCACCGACTTGCTCACGCAATTCACGATAAAGGGTACTGGCAATAAGACTCTTGCCGGAAGCCGATGCGCCAGCGATACCGATAATGACGCACTGATGAGACTGATCAGTCATATATTTAGCGACCTGATTAAc                                                                                                                          >  1:335130/1‑150 (MQ=255)

                               cGCCGATGTGTTCATCACCGACTTGCTCACGCAATTCACGATAAAGGGTACTGGCAATAAGACTCTTGCCGGAAGCCGATGCGCCAGCGATACCGATAATGACGCACTGATGAGACTGATCAGTCATATATTTAGCGACCTGATTAACCt                                                                                                                        >  2:4854368/1‑150 (MQ=255)

                                gCCGATGTGTTCATCACCGACTTGCTCACGCAATTCACGATAAAGGGTACTGGCAATAAGACTCTTGCCGGAAGCCGATGCGCCAGCGATACCGATAATGACGCACTGATGAGACTGATCAGTCATATATTTAGCGACCTGATTAACCTg                                                                                                                       >  2:4526173/1‑150 (MQ=255)

                                gCCGATGTGTTCATCACCGACTTGCTCACGCAATTCACGATAAAGGGTACTGGCAATAAGACTCTTGCCGGAAGCCGATGCGCCAGCGATACCGATAATGACGCACTGATGAGACTGATCAGTCATATATTTAGCGACCTGATTAACCTg                                                                                                                       >  2:2128558/1‑150 (MQ=255)

                                   gATGTGTTCATCACCGACTTGCTCACGCAATTCACGATAAAGGGTACTGGCAATAAGACTCTTGCCGGAAGCCGATGCGCCAGCGATACCGATAATGACGCACTGATGAGACTGATCAGTCATATATTTAGCGACCTGATTAACCTGGAt                                                                                                                    >  2:4418401/1‑150 (MQ=255)

                                    aTGTGTTCATCACCGACTTGCTCACGCAATTCACGATAAAGGGTACTGGCAATAAGACTCTTGCCGGAAGCCGATGCGCCAGCGATACCGATAATGACGCACTGATGAGACTGATCAGTCATATATTTAGCGACCTGATTAACCTGGATg                                                                                                                   >  2:1307651/1‑150 (MQ=255)

                                      gtgtTCATCACCGACTTGCTCACGCAATTCACGATAAAGGGTACTGGCAATAAGACTCTTGCCGGAAGCCGATGCGCCAGCGATACCGATAATGACGCACTGATGAGACTGATCAGTCATATATTTAGCGACCTGATTAACCTGGATGtt                                                                                                                 <  2:298568/150‑1 (MQ=255)

                                           catcaCCGACTTGCTCACGCAATTCACGATAAAGGGTACTGGCAATAAGACTCTTGCCGGAAGCCGATGCGCCAGCGATACCGATAATGACGCACTGATGAGACTGATCAGTCATATATTTAGCGACCTGATTAACCTGGATGTTAGGaa                                                                                                            <  1:3726355/150‑1 (MQ=255)

                                            atcaCCGACTTGCTCACGCAATTCACGATAAAGGGTACTGGCAATAAGACTCTTGCCGGAAGCCGATGCGCCAGCGATACCGATAATGACGCACTGATGAGACTGATCAGTCATATATTTAGCGACCTGATTAACCTGGATGTTAGGAAg                                                                                                           <  1:699950/150‑1 (MQ=255)

                                            atcaCCGAATTGCTCACGCAATTCACGATAAAGGGTACTGGCAATAAGACTCTTGCCGGAAGCCGATGCGCCAGCGATACCGATAATGACGCACTGATGAGACTGATCAGTCATATATTTAGCGACCTGATTAACCTGGATGTTAGGAAg                                                                                                           <  2:4270492/150‑1 (MQ=255)

                                             tcaCCGACTTGCTCACGCAATTCACGATAAAGGGTACTGGCAATAAGACTCTTGCCGGAAGCCGATGCGCCAGCGATACCGATAATGACGCACTGATGAGACTGATCAGTCATATATTTAGCGACCTGATTAACCTGGATGTTAGGAAgg                                                                                                          >  2:1805534/1‑150 (MQ=255)

                                             tcaCCGACTTGCTCACGCAATTCACGATAAAGGGTACTGGCAATAAGACTCTTGCCGGAAGCCGATGCGCCAGCGATACCGATAATGACGCACTGATGAGACTGATCAGTCATATATTTAGCGACCTGATTAACCTGGATGTTAGGAAgg                                                                                                          >  1:4311170/1‑150 (MQ=255)

                                              caCCGACTTGCTCACGCAATTCACGATAAAGGGTACTGGCAATAAGACTCTTGCCGGAAGCCGATGCGCCAGCGATACCGATAATGACGCACTGATGAGACTGATCAGTCATATATTTAGCGACCTGATTAACCTGGATGTTAGGAAggg                                                                                                         >  1:3946993/1‑150 (MQ=255)

                                              caCCGACTTGCTCACGCAATTCACGATAAAGGGTACTGGCAATAAGACTCTTGCCGGAAGCCGATGCGCCAGCGATACCGATAATGACGCACTGATGAGACTGATCAGTCATATATTTAGCGACCTGATTAACCTGGATGTTAGGAAggg                                                                                                         >  1:3947063/1‑150 (MQ=255)

                                               aCCGACTTGCTCACGCAATTCACGATAAAGGGTACTGGCAATAAGACTCTTGCCGGAAGCCGATGCGCCAGCGATACCGATAATGACGCACTGATGAGACTGATCAGTCATATATTTAGCGACCTGATTAACCTGGATGTTAGGAAgggg                                                                                                        <  1:4219182/150‑1 (MQ=255)

                                                 cGACTTGCTCACGCAATTCACGATAAAGGGTACTGGCAATAAGACTCTTGCCGGAAGCCGATGCGCCAGCGATACCGATAATGACGCACTGATGAGACTGATCAGTCATATATTTAGCGACCTGATTAACCTGGATGTTAGGAAGGGGGc                                                                                                      <  1:3059645/150‑1 (MQ=255)

                                                 cGACTTGCTCACGCAATTCACGATAAAGGGTACTGGCAATAAGACTCTTGCCGGAAGCCGATGCGCCAGCGATACCGATAATGACGCACTGATGAGACTGATCAGTCATATATTTAGCGACCTGATTAACCTGGATGTTAGGAAGGGGGc                                                                                                      <  1:4623108/150‑1 (MQ=255)

                                                  gACTTGCTCACGCAATTCACGATAAAGGGTACTGGCAATAAGACTCTTGCCGGAAGCCGATGCGCCAGCTATACCGATAATGACGCACTGTTGAGACTGATCAGTCATATATTTAGCGACCTGATTAACCTGGATGTTAGGAAGGGGGcg                                                                                                     <  2:2957866/150‑1 (MQ=255)

                                                     ttGCTCACGCAATTCACGATAAAGGGTACTGGCAATAAGACTCTTGCCGGAAGCCGATGCGCCAGCGATACCGATAATGACGCACTGATGAGACTGGACAGTCATATATTTAGCGACCTGATTAACCTGGATGTTAGGAAGGGGGcgacg                                                                                                  <  1:4539418/150‑1 (MQ=255)

                                                     ttGCTCACGCAATTCACGATAAAGGGTACTGGCAATAAGACTCTTGCCGGAAGCCGATGCGCCAGCGATACCGATAATGACGCACTGATGAGACTGATCAGTCATATATTTAGCGACCTGATTAACCTGGATGTTAGGAAGGGGGcgacg                                                                                                  >  2:3699949/1‑150 (MQ=255)

                                                     ttGCTCACGCAATTCACGATAAAGGGTACTGGCAATAAGACTCTTGCCGGAAGCCGATGCGCCAGCGATACCGATAATGACGCACTGATGAGACTGATCAGTCATATATTTAGCGACCTGATTAACCTGGATGTTAGGAAGGGGGcgacg                                                                                                  >  1:2651569/1‑150 (MQ=255)

                                                      tGCTCACGCAATTCACGATAAAGGGTACTGGCAATAAGACTCTTGCCGGAAGCCGATGCGCCAGCGATACCGATAATGACGCACTGATGAGACTGATCAGTCATATATTTAGCGACCTGATTAACCTGGATGTTAGGAAGGGGGcgacga                                                                                                 >  1:76538/1‑150 (MQ=255)

                                                          cACGCAATTCACGATAAAGGGTACTGGCAATAAGACTCTTGCCGGAAGCCGATGCGCCAGCGATACCGATAATGACGCACTGATGAGACTGATCAGTCATATATTTAGCGACCTGATTAACCTGGATGTTAGGAAGGGGGCGACGAAgcg                                                                                             <  1:2427506/150‑1 (MQ=255)

                                                               aaTTCACGATAAAGGGTACTGGCAATAAGACTCTTGCCGGAAGCCGATGCGCCAGCGATACCGATAATGACGCACTGATGAGACTGATCAGTCATATATTTAGCGACCTGATTAACCTGGATGTTAGGAAGGGGGCGACGAAGCGCCaaa                                                                                        >  2:1714034/1‑150 (MQ=255)

                                                               aaTTCACGATAAAGGGTACTGGCAATAAGACTCTTGCCGGAAGCCGATGCGCCAGCGATACCGATAATGACGCACTGATGAGACTGATCAGTCATATATTTAGCGACCTGATTAACCTGGATGTTAGGAAGGGGGCGACGAAGCGCCaaa                                                                                        >  1:2537784/1‑150 (MQ=255)

                                                               aaTTCACGATAAAGGGTACTGGCAATAAGACTCTTGCCGGAAGCCGATGCGCCAGCGATACCGATAATGACGCACTGATGAGACTGATCAGTCATATATTTAGCGACCTGATTAACCTGGATGTTAGGAAGGGGGCGACGAAGCGCCaaa                                                                                        >  1:3548286/1‑150 (MQ=255)

                                                                aTTCACGATAAAGGGTACTGGCAATAAGACTCTTGCCGGAAGCCGATGCGCCAGCGATACCGATAATGACGCACTGATGAGACTGATCAGTCATATATTTAGCGACCTGATTAACCTGGATGTTAGGAAGGGGGCGACGAAGCGCCAAAc                                                                                       >  1:4109928/1‑150 (MQ=255)

                                                                aTTCACGATAAAGGGTACTGGCAATAAGACTCTTGCCGGAAGCCGATGCGCCAGCGATACCGATAATGACGCACTGATGAGACTGATCAGTCATATATTTAGCGACCTGATTAACCTGGATGTTAGGAAGGGGGCGACGAAGCGCCAAAc                                                                                       >  1:4035393/1‑150 (MQ=255)

                                                                aTTCACGATAAAGGGTACTGGCAATAAGACTCGTGCCGGAAGCCGATGCGCCAGCGATACCGATAATGACGCACTGATGAGACTGATCAGTCATATATTTAGCGACCTGATTAACCTGGATGTTAGGAAGGGGGCGACGAAGCGCCAAAc                                                                                       <  2:3757807/150‑1 (MQ=255)

                                                                    aCGATAAAGGGTACTGGCAATAAGACTCTTGCCGGAAGCCGATGCGCCAGCGATACCGATAATGACGCACTGATGAGACTGATCAGTCATATATTTAGCGACCTGATTAACCTGGATGTTAGGAAGGGGGCGACGAAGCGCCAAACGCgg                                                                                   <  2:943848/150‑1 (MQ=255)

                                                                     cGATAAAGGGTACTGGCAATAAGACTCTTGCCGGAAGCCGCTGCGCCAGCGATACCGATAATGACGCACTGATGAGACTGATCAGTCATATATTTAGCGACCTGATTAACCTGGATGTTAGGAAGGGGGCGACGAAGCGCCAAACGCGGc                                                                                  <  2:2746593/150‑1 (MQ=255)

                                                                     cGATAAAGGGTACTGGCAATAAGACTCTTGCCGGAAGCCGATGCGCCAGCGATACCGATAATGACGCACTGATGAGACTGATCAGTCATATATTTAGCGACCTGATTAACCTGGATGTTAGGAAGGGGGCGACGAAGCGCCAAACGCGGc                                                                                  <  2:3341762/150‑1 (MQ=255)

                                                                      gATAAAGGGTACTGGCAATAAGACTCTTGCCGGAAGCCGATGCGCCAGCGATACCGATAATGACGCACTGATGAGACTGGTCAGTCATATATTTAGCGACCTGATTAACCTGGATGTTAGGAAGGGGGCGACGAAGCGCCAAACGCGGCa                                                                                 >  2:4077083/1‑150 (MQ=255)

                                                                      gATAAAGGGTACTGGCAATAAGACTCTTGCCGGAAGCCGATGCGCCAGCGATACCGATAATGACGCACTGATGAGACTGATCAGTCATATATTTAGCGACCTGATTAACCTGGATGTTAGGAAGGGGGCGACGAAGCGCCAAACGCGGCa                                                                                 <  1:1638279/150‑1 (MQ=255)

                                                                      gATAAAGGGTACTGGCAATAAGACTCTTGCCGGAAGCCGATGCGCCAGCGATACCGATAATGACGCACTGATGAGACTGATCAGTCATATATTTAGCGACCTGATTAACCTGGATGTTAGGAAGGGGGCGACGAAGCGCCAAACGCGGCa                                                                                 <  2:1725443/150‑1 (MQ=255)

                                                                        tAAAGGGTACTGGCAATAAGACTCTTGCCGGAAGCCGATGCGCCAGCGATACCGATATTGACGCACTGATGAGACTGATCAGTCATATATTTAGCGACCTGATTAACCTGGATGTTAGGAAGGGGGCGACGAAGCGCCAAACGCGGCAAt                                                                               <  1:2205152/150‑1 (MQ=255)

                                                                        tAAAGGGTACTGGCAATAAGACTCTTGCCGGAAGCCGATGCGCCAGCGATACCGATAATGACGCACTGATGAGACTGATCAGTCATATATTTAGCGACCTGATTAACCTGGATGTTAGGAAGGGGGCGACGAAGCGCCAAACGCGGCAAt                                                                               >  1:3582672/1‑150 (MQ=255)

                                                                         aaaGGGTACTGGCAATAAGACTCTTGCCGGAAGCCGATGCGCCAGCGATACCGATAATGACGCACTGATGAGACTGATCAGTCATATATTTAGCGACCTGATTAACCTGGATGTTAGGAAGGGGGCGACGAAGCGCCAAACGCGGCAAtt                                                                              >  1:2772640/1‑150 (MQ=255)

                                                                         aaaGGGTACTGGCAATAAGACTCTTGCCGGAAGCCGATGCGCCAGCGATACCGATAATGACGCACTGATGAGACTGATCAGTCATATATTTAGCGACCTGATTAACCTGGATGTTAGGAAGGGGGCGACGAAGCGCCAAACGCGGCAAtt                                                                              >  2:1665196/1‑150 (MQ=255)

                                                                           aGGGTACTGGCAATAAGACTCTTGCCGGAAGCCGATGCGCCAGCGATACCGATAATGACGCACTGATGAGACTGATCAGTCATATATTTAGCGACCTGATTAACCTGGATGTTAGGAAGGGGGCGACGAAGCGCCAAACGCGGCAATtat                                                                            <  2:3617426/150‑1 (MQ=255)

                                                                            gggTACTGGCAATAAGACTCTTGCCGGAAGCCGATGCGCCAGCGATACCGATAATGACGCACTGATGAGACTGGTCAGTCATATATTTAGCGACCTGATTAACCTGGATGTTAGGAAGGGGGCGACGAAGCGCCAAACGCGGCAATtata                                                                           <  1:512699/150‑1 (MQ=255)

                                                                            gggTACTGGCAATAAGACTCTTGCCGGAAGCCGATGCGCCAGCGACACCGATAATGACGCACTGATGAGACTGGTCAGTCATATATTTAGCGACCTGATTAACCTGGATGTTAGGAAGGGGGCGACGAAGCGCCAAACGCGGCAATtata                                                                           <  2:404042/150‑1 (MQ=255)

                                                                              gTACTGGCAATAAGACTCTTGCCGGAAGCCGATGCGCCAGCGATACCGATAATGACGCACTGATGAGACTGATCAGTCATATATTTAGCGACCTGATTAACCTGGATGTTAGGAAGGGGGCGACGAAGCGCCAAACGCGGCACTTATAgg                                                                         >  2:5094208/1‑150 (MQ=255)

                                                                                   ggCAATAAGACTCTTGCTGGAAGCCGAGGCGCCAGCGATACCGATAATGACGCACTGATGAGACTGATCAGTCATATATTTAGCGACCTGATTAACCTGGATGTTAGGAAGGGGGCGACGAAGCGCCAAACGCGGCAATTATAGGGAttt                                                                    <  1:3525183/150‑1 (MQ=255)

                                                                                    gCAATAAGACTCTTGCCGGAAGCCGATGCGCCAGCGATACCGATAATGACGCACTGATGAGACTGATCAGTCATATATTTAGCGACCTGATTAACCTGGATGTTAGGAAGGGGGCGACGAAGCGCCAAACGCGGCAATTATAGGGATTTc                                                                   <  1:1942991/150‑1 (MQ=255)

                                                                                     cAATAAGACTCGTGCCGGAAGCCGATGCGCCAGCGATACCGATAATGACGCACTGATGAGACTGATCAGTCATATATTTAGCGACCTGATTAACCTGGATGTTAGGAAGGGGGCGACGAAGCGCCAAACGCGGCAATTATAGGGATTTCa                                                                  <  2:2341762/150‑1 (MQ=255)

                                                                                       aTAAGACTCTTGCCGGAAGCCGATGCGCCAGCGATACCGATAATGACGCACTGATGAGACTGATCAGTCATATATTTAGCGACCTGATTAACCTGGATGTTAGGAAGGGGGCGACGAAGCGCCAAACGCGGCAATTATAGGGATTTCATc                                                                >  1:2376234/1‑150 (MQ=255)

                                                                                         aaGACTCTTGCCGGAAGCCGATGCGCCAGCGATACCGATAATGACGCACTGATGAGACTGGTCAGTCATATATTTAGCGACCTGATTAACCTGGATGTTAGGAAGGGGGCGACGAAGCGCCAAACGCGGCAATTATAGGGATATCATCCg                                                              >  2:651913/1‑150 (MQ=255)

                                                                                            aCTCTTGCCGGAAGCCGATGCGCCAGCGATACCGATAATGACGCACTGATGAGACTGATCAGTCATATATTTAGCGACCTGATTAACCTGGATGTTAGGAAGGGGGCGACGAAGCGCCAAACGCGGCAATTATAGGGATTTCATCCGTCt                                                           >  2:2215294/1‑150 (MQ=255)

                                                                                             ctctTGCCGGAAGCCGATGCGCCAGCGATACCGATAATGACGCACTGATGAGACTGATCAGTCATATATTTAGCGACCTGATTAACCTGGATGTTAGGAAGGGGGCGACGAAGCGCCAAACGCGGCAATTATAGGGATTTCATCCGTCTg                                                          <  2:1931730/150‑1 (MQ=255)

                                                                                               ctTGCCGGAAGCCGATGCGCCAGCGATACCGATAATGACGCACTGATGAGACTGATCAGTCATATATTTAGCGACCTGATTAACCTGGATGTTAGGAAGGGGGCGACGAAGCGCCAAACGCGGCAATTATAGGGATTTCATCCGTCTGAt                                                        >  2:3179693/1‑150 (MQ=255)

                                                                                                         gCCGATGCGCCAGCGATACCGATAATGACGCACTGATGAGACTGATCAGTCATATATTTAGCGACCTGATTAACCTGGATGTTAGGAAGGGGGCGACGAAGCGCCAAACGCGGCAATTATAGGGATTTCATCCGTCTGATACCAGTCGaa                                              <  1:9146/150‑1 (MQ=255)

                                                                                                           cGATGCGCCAGCGATACCGATAATGACGCACTGATGAGACTGATCAGTCATATATTTAGCGACCTGATTAACCTGGATGTTAGGAAGGGGGCGACGAAGCGCCAAACGCGGCAATTATAGGGATTTCATCCGTCTGATACCAGTCGAATa                                            <  2:2886307/150‑1 (MQ=255)

                                                                                                             aTGCGCCAGCGATACCGATAATGACGCACTGATGAGACTGATCAGTCATATATTTAGCGACCTGATTAACCTGGATGTTAGGAAGGGGGCGACGAAGCGCCAAACGCGGCAATTATAGGGATTTCATCCGTCTGATACCAGTCGAATAGc                                          >  1:4828376/1‑150 (MQ=255)

                                                                                                                    aGCGATACCGATAATGACGCACTGATGAGACTGATCAGTCATATATTTAGCGACCTGATTAACCTGGATGTTAGGAAGGGGGCGACGAAGCGCCAAACGCGGCAATTATAGGGATTTCATCCGTCTGATACCAGTCGAATAGCGTTGCcg                                   <  1:1247010/150‑1 (MQ=255)

                                                                                                                     gCGATACCGATAATGACGCACTGATGAGACTGATCAGTCATATATTTAGCGACCTGATTAACCTGGATGTTAGGAAGGGGGCGACGAAGCGCCAAACGCGGCAATTATAGGGATTTCATCCGTCTGATACCAGTCGAATAGCGTTGCcgc                                  <  1:2329083/150‑1 (MQ=255)

                                                                                                                     gCGATACCGATAATGACGCACTGATGAGACTGATCAGTCATATATTTAGCGACCTGATTAACCTGGATGTTAGGAAGGGGGCGACGAAGCGCCAAACGCGGCAATTATAGGGATTTCATCCGTCTGATACCAGTCGAATAGCGTTGCcgc                                  <  1:2035094/150‑1 (MQ=255)

                                                                                                                               tAATGACGCACTGATGAGACTGGTCAGTCATATATTTAGCGACCTGATTAACCTGGATGTTAGGAAGGGGCCGACGAAGCGCAAAACGCGGCAATTATAGGGATTTCATCCGTCTGATACCAGTCGAATAGCGTTGCCGCGCGCTCagag                        <  1:1753872/150‑1 (MQ=255)

                                                                                                                               tAATGACGCACTGATGAGACTGATCAGTCATATATTTAGCGACCTGATTAACCTGGATGTTAGGAAGGGGGCGACGAAGCGCCAAACGCGGCAATTATAGGGATTTCATCCGTCTGATACCAGTCGAATAGCGTTGCCGCGCGCTCagag                        <  2:2063175/150‑1 (MQ=255)

                                                                                                                                aaTGACGCACTGATGAGACTGATCAGTCATATATTTAGCGACCTGATTAACCTGGATGTTAGGAAGGGGGCGACGAAGCGCCAAACGCGGCAATTATAGGGATTTCATCCGTCTGATACCAGTCGAATAGCGTTGCCGCGCGCTCAGAGt                       >  1:1726831/1‑150 (MQ=255)

                                                                                                                                aaTGACGCACTGATGAGACTGATCAGTCATATATTTAGCGACCTGATTAACCTGGATGTTAGGAAGGGGGCGACGAAGCGCCAAACGCGGCAATTATAGGGATTTCATCCGTCTGATACCAGTCGAATAGCGTTGCCGCGCGCTCAGAGt                       >  1:1215975/1‑150 (MQ=255)

                                                                                                                                  tGACGCACTGATGAGACTGATCAGTCATATATTTAGCGACCTGATTAACCTGGATGTTAGGAAGGGGGCGACGAAGCGCCAAACGCGGCAATTATAGGGATTTCATCCGTCTGATACCAGTCGAATAGCGTTGCCGCGCGCTCAGAGTTa                     >  2:793280/1‑150 (MQ=255)

                                                                                                                                   gACGCACTGATGAGACTGATCAGTCATATATTTAGCGACCTGATTAACCTGGATGTTAGGAAGGGGGCGACGAAGCGCCAAACGCGGCAATTATAGGGATTTCATCCGTCTGATACCAGTCGAATAGCGTTGCCGCGCGCTCAGAGTTaa                    >  1:4007065/1‑150 (MQ=255)

                                                                                                                                    aCGCACTGATGAGACTGATCAGTCATATATTTAGCGACCTGATTAACCTGGATGTTAGGAAGGGGGCGACGAAGCGCCAAACGCGGCAATTATAGGGATTTCATCCGTCTGATACCAGTCGAATAGCGTTGCCGCGCGCTCAGAGTTAAt                   <  1:2846680/150‑1 (MQ=255)

                                                                                                                                    aCGCACTGATGAGACTGATCAGTCATATATTTAGCGACCTGATTAACCTGGATGTTAGGAAGGGGGCGACGAAGCGCCAAACGCGGCAATTATAGGGATTTCATCCGTCTGATACCAGTCGAATAGCGTTGCCGCGCGCTCAGAGTTAAt                   >  1:4482077/1‑150 (MQ=255)

                                                                                                                                    aCGCACTGATGAGACTGATCAGTCATATATTTAGCGACCTGATTAACCTGGATGTTAGGAAGGGGGCGACGAAGCGCCAAACGCGGCAATTATAGGGATTTCATCCGTCTGATACCAGTCGAATAGCGTTGCCGCGCGCTCAGAGTTAAt                   <  2:3823092/150‑1 (MQ=255)

                                                                                                                                     cGCACTGATGAGACTGATCAGTCATATATTTAGCGACCTGATTAACCTGGATGTTAGGAAGGGGGCGACGAAGCGCCAAACGCGGCAATTATAGGGATTTCATCCGTCTGATACCAGTCGAATAGCGTTGCCGCGCGCTCAGAGTTAAtt                  <  1:2778550/150‑1 (MQ=255)

                                                                                                                                     cGCACTGATGAGACTGATCAGTCATATATTTAGCGACCTGATTAACCTGGATGTTAGGAAGGGGGCGACGAAGCGCCAAACGCGGCAATTATAGGGATTTCATCCGTCTGATACCAGTCGAATAGCGTTGCCGCGCGCTCAGAGTTAAtt                  <  1:630684/150‑1 (MQ=255)

                                                                                                                                        aCTGATGAGACTGATCAGTCATATATTTAGCGACCTGATTAACCTGGATGTTAGGAAGGGGGCGACGAAGCGCCAAACGCGGCAATTATAGGGATTTCATCCGTCTGATACCAGTCGAATAGCGTTGCCGCGCGCTCAGAGTTAAttgtt               <  1:75523/150‑1 (MQ=255)

                                                                                                                                         cTGATGAGACTGATCAGTCATATATTTAGCGACCTGATTAACCTGGATGTTAGGAAGGGGGCGACGAAGCGCCAAACGCGGCAATTATAGGGATTTCATCCGTCTGATACCAGTCGAATAGCGTTGCCGCGCGCTCAGAGTTAAttgttg              >  1:1923337/1‑150 (MQ=255)

                                                                                                                                         cTGATGAGACTGATCAGTCATATATTTAGCGACCTGATTAACCTGGATGTTAGGAAGGGGGCGACGAAGCGCCAAACGCGGCAATTATAGGGATTTCATCCGTCTGATACCAGTCGAATAGCGTTGCCGCGCGCTCAGAGTTAAttgttg              >  2:494437/1‑150 (MQ=255)

                                                                                                                                          tgatgaGACTGATCAGTCATATATTTAGCGACCTGATTAACCTGGATGTTAGGAAGGGGGCGACGAAGCGCCAAACGCGGCAATTATAGGGATTTCATCCGTCTGATACCAGTCGAATAGCGTTGCCGCGCGCTCAGAGTTAATTGTTGa             >  2:2440521/1‑150 (MQ=255)

                                                                                                                                               agaCTGGTCAGTCATATATTTAGCGACCTGATTAACCTGGATGTTAGGAAGGGGGCGACGAAGCGCCAAACGCGGCAATTATAGGGATTTCATCCGTCTGATACCAGTCGAATAGCGTTGCCGCGCGCTCAGAGTTAATTGTTGACAAAg        <  2:4713984/150‑1 (MQ=255)

                                                                                                                                                gaCTGGTCAGTCATATATTTAGCGACCTGATTAACCTGGATGTTAGGAAGGGGGCGACGAAGCGCCAAACGCGGCAATTATAGGGATTTCATCCGTCTGATACCAGTCGAATAGCGTTGCCGCGCGCTCAGAGTTAATTGTTGACAAAGa       >  1:4636895/1‑150 (MQ=255)

                                                                                                                                                gaCTGATCAGTCATATATTTAGCGACCTGATTAACCTGGATGTTAGGAAGGGGGCGACGAAGCGCCAAACGCGGCAATTATAGGGATTTCATCCGTCTGATACCAGTCGAATAGCGTTGCCGCGCGCTCAGAGTTAATTGTTGACAAAGa       >  2:3807181/1‑150 (MQ=255)

                                                                                                                                                gaCTGATCAGTCATATATTTAGCGACCTGATTAACCTGGATGTTAGGAAGGGGGCGACGAAGCGCCAAACGCGGCAATTATAGGGATTTCATCCGTCTGATACCAGTCGAATAGCGTTGCCGCGCGCTCAGAGTTAATTGTTGACAAAGa       <  2:2682706/150‑1 (MQ=255)

                                                                                                                                                 aCTGATCAGTCATATATTTAGCGACCTGATTAACCTGGATGTTAGGAAGGGGGCGACGAAGCGCCAAACGCGGCAATTATAGGGATTTCATCCGTCTGATACCAGTCGAATAGCGTTGCCGCGCGCTCAGAGTTAATTGTTGACAAAGaa      <  2:735495/150‑1 (MQ=255)

                                                                                                                                                 aCTGATCAGTCATATATTTAGCGACCTGATTAACCTGGATGTTAGGAAGGGGGCGACGAAGCGCCAAACGCGGCAATTATAGGGATTTCATCCGTCTGATACCAGTCGAATAGCGTTGCCGCGCGCTCAGAGTTAATTGTTGACAAAGaa      >  2:1193532/1‑150 (MQ=255)

                                                                                                                                                  cTGATCAGTCATATATTTAGCGACCTGATTAACCTGGATGTTAGGAAGGGGGCGACGAAGCGCCAAACGCGGCAATTATAGGGATTTCATCCGTCTGATACCAGTCGAATAGCGTTGCCGCGCGCTCAGAGTTAATTGTTGACAAAGAAt     <  1:3812083/150‑1 (MQ=255)

                                                                                                                                                   tGATCAGTCATATATTTAGCGACCTGATTAACCTGGATGTTAGGAAGGGGGCGACGAAGCGCCAAACGCGGCAATTATAGGGATTTCATCCGTCTGATACCAGTCGAATAGCGTTGCCGCGCGCTCAGAGTTAATTGTTGACAAAGAAtt    >  1:2009956/1‑150 (MQ=255)

                                                                                                                                                   tGATCAGTCATATATTTAGCGACCTGATTAACCTGGATGTTAGGAAGGGGGCGACGAAGCGCCAAACGCGGCAATTATAGGGATTTCATCCGTCTGATACCAGTCGAATAGCGTTGCCGCGCGCTCAGAGTTAATTGTTGACAAAGAAtt    >  1:1026655/1‑150 (MQ=255)

                                                                                                                                                     aTCAGTCATATATTTAGCGACCTGATTAACCTGGATGTTAGGAAGGGGGCGACGAAGCGCCAAACGCGGCAATTATAGGGATTTCATCCGTCTGATACCAGTCGAATAGCGTTGCCGCGCGCTCAGAGTTAATTGTTGACAAAGAATTcc  <  1:3272631/150‑1 (MQ=255)

TGATCTTTGTAATAGCAGTCTTCGGGAATTACGCCGATGTGTTCATCACCGACTTGCTCACGCAATTCACGATAAAGGGTACTGGCAATAAGACTCTTGCCGGAAGCCGATGCGCCAGCGATACCGATAATGACGCACTGATGAGACTGATCAGTCATATATTTAGCGACCTGATTAACCTGGATGTTAGGAAGGGGGCGACGAAGCGCCAAACGCGGCAATTATAGGGATTTCATCCGTCTGATACCAGTCGAATAGCGTTGCCGCGCGCTCAGAGTTAATTGTTGACAAAGAATTCC  >  CP009273/2136272‑2136570

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`